FIELD OF THE INVENTION:
The present invention relates to oil-based agrochemical composition comprising bioactive amounts of (A) an insecticide Diafenthiuron; (B) an insecticide selected from Tolfenpyrad or Flonicamid. The present invention further relates to process of preparing the said oil-based agrochemical composition along with at least one inactive excipients and formulation thereof.
BACKGROUND OF THE INVENTION:
Combination of insecticides are used to broaden the spectrum of control of insect, to improve the insect-pest control with synergistc effect, reduce dosage, thereby reducing environmental impact, to broaden the spectrum of control, i.e. chewing and sucking insects at a time, decrease chances of resistance development and management of resistance and to enhance residual control so lesser the number of sprays for crop protections and minimizing the pesticidal load in ecosystem. The combination of insecticides at times demonstrate an additive or synergistic effect that results in an improved control on the insect-pests and mites.
Insecticide or pesticides are used widely and very frequently in commercial agriculture and have enabled an enormous increase in crop yields and product quality which ultimately increased the ease to farmers in term of economic advantage as well as ease of farming activities.
Insecticidal combination are used usually to broaden the spectrum of activities, controls insects-pest and mites. Further the insecticidal combination improves the residual control of target insects-pests and delay the onset of the resistance. In agriculture it controls and prevents the damage by insects-pests and mites.
There are many combinations of insecticide known in the art for the control of insect-pests. For example, CN103271051A patent relates a tolfenpyrad-diafenthiuron composite insecticide composition having synergism. The tolfenpyrad-diafenthiuron composite insecticide composition having synergism comprises two active ingredients such as tolfenpyrad and diafenthiuron and also comprises auxiliary agents or carriers. Further it discloses suspending agent and formulations like water dispersible granules (WDG) or wetting powder (WP).

CN106359421A patent relates to a diafenthiuron and tolfenpyrad compound suspension agent and a preparation method thereof. The invention relates to the technical field of prevention and treatment of tea lesser leafhoppers on tea trees. Further it discloses suspending agents for the formulation.
CN102630677A patent relates to tolfenpyrad-containing ultra-low volume liquid and tolfenpyrad and an active component are prepared into an active component, and auxiliaries and solvents are supplemented to 100% to obtain the ultra-low volume liquid. Further it contains active components which discloses diafenthiuron as one of the component.
CN103053569A patent relates to a tolfenpyrad-containing insecticidal composition. The tolfenpyrad-containing insecticidal composition comprises composition of tolfenpyrad and diafenthiuron in a specific ratio; and the balance of auxiliary ingredient of pesticide preparation. Further the formulation for the composition can be prepared as missible oil, aqueous emulsion, microemulsion, wetting powder, water dispersible granules or suspending agents.
CN103766363 A patent relates to an insecticide composition containing flonicamid and diafenthiuron. The insecticide composition comprises effective components including the flonicamid, the diafenthiuron and a frequently used auxiliary component. Further the formulation for the composition disclosed are suspending agent, aqueous emulsion, microemulsion, water dispersible granules, wetting powder and ultralow volume liquids.
There is however a need for improvement of these combinations. Single active combinations used over a long period of time has resulted in resistance. With the onset of resistance to certain pests, there is a need in the art for a combination of actives that decreases chances of resistance and improves the spectrum of insect-pest and mites control.
However still there is a need for a composition comprises an insecticide from acaricide thiourea group (diafenthiuron); and an insecticide selected from pyrazole group of insecticide (tolfenpyrad) or from pyridinecarboxamid (flonicamid) which overcomes the existing problems and can be prepared easily without much complex manufacturing process.

In general use, the insecticides actives are used in the form of a dilute aqueous composition because it can attain a good interaction with the target insects. However, most active pesticide compounds that are used as pesticides are only sparingly or even insoluble in water. The low solubility of such compounds present the challenges and difficulties to formulator in formulating insecticide compounds in stable formulations that can be easily stored for a long time and which still have a high stability and effective activity until end use. This problem especially occurs and may get worsen if more than one active compound is present in the composition.
Therefore, one object of the present invention is to provide improved combinations of insecticides for the control of insect-pests and mites. Another object of the present invention is to provide a method and a composition for controlling insect pests. Further objective is to provide the formulations that are stable, non- phytotoxic, non-skin irritant and environmentally safe.
Yet another object of the present invention is to provide improved combinations of insecticides that promote plant health.
Embodiment of the present invention can ameliorate one or more of the above mentioned problems.
Inventors of the present invention have surprisingly found that the novel synergistic insecticidal composition of diafenthiuron and tolfenpyrad; or diafenthiuron and flonicamid in oil dispersion formulation as described herein which can provide solution to the above mentioned problems.
SUMMARY OF THE INVENTION
In an embodiment of the present invention is to provide oil-based agrochemical composition comprising of two insecticides.
Another embodiment of the present invention discloses oil-based agrochemical composition comprising of bioactive amounts of a) an insecticide Diafenthiuron; and b) an insecticide selected from Tolfenpyrad or Folicamid.

Another embodiment of the present invention provides oil-baased agrochemical composition comprising of bioactive amounts of a) an insecticide Diafenthiuron; b) an insecticide selected from Tolfenpyrad or Folonicamid with one or more inactive excipients.
Still another embodiment of the present invention provides oil-based agrochemical composition comprising of bioactive amounts of a) Diafenthiuron b) an insecticide selected from Tolfenpyrad or Flonicamid which is stable, non-phytotoxic, non-skin irritant and environmental safe for use.
Still further embodiment of the present invention provides oil-based agrochemical composition comprising of a) Diafenthiuron b) an insecticide selected from Tolfenpyrad or Flonicamid wherein the said composition is in oil dispersion formulation.
Still another embodiment of the present invention provides oil-based agrochemical composition comprising of a) Diafenthiuron b) an insecticide selected from Tolfenpyrad or Flonicamid which provides broad spectrum of activity, controls insects-pests and mites. Further the present composition and formulation thereof improve residual control of target insect-pests and delay the onset of insecticidal resistance.
In an aspect of the present insecticidal composition Oil Dispersion (OD) formulation comprises of vegetables oil as carrier that increases penetration into leaf surfaces, especially plant, improves retention of active ingredients on leaf surfaces, increases spreading action, so faster spray coverage on leaf surface and easily dissolve the waxy layer of the leaf surface.
The Oil Dipsersion formulation of the present insecticidal composition increases the bioefficacy of targets insect-pests with reduced dose of active ingredients.
DETAILED DESCRIPTION OF THE INVENTION:
The term "synergistic", as used herein, refers the combined action of two or more active agents blended together and administered conjointly that is greater than the sum of their individual effects.

"Bioactive amounts" as mentioned herein means that amount which, when applied treatment of crops, is sufficient to effect such treatment.
In an aspect of the present invention provides an oil-based agrochemical composition comprising bioactive amounts of (A) an insecticide Diafenthiuron; (B) an insecticide selected from Tolfenpyrad or Flonicamid.
Diafenthiuron:
Diafenthiuron is an aromatic ether that is 1, 3-diisopropyl-5-phenoxybenzene in which the hydrogen atom at position 2 is substituted by a (tert-butylcarbamothioyl) nitrilo group. An agricultural proinsecticide which is used to control mites, aphids and whitefly in cotton. It has a role as an oxidative phosphorylation inhibitor and a proinsecticide. It is a thiourea acaricide, a thiourea insecticide and an aromatic ether. It derives from a diphenyl ether.
It has an IUPAC name as l-tert-butyl-3-[4-phenoxy-2,6-di(propan-2-yl)phenyl]thiourea and has a chemical structure as follows:
H3C
J^ H H CH3 H3C CH3
Diafenthiuron comes under category of insecticide, acaricide and miticide. Diafenthiuron is a new insecticide with novel mode of action of inhibiting mitochondrial ATPase and acaricidal action.Further it has a broad spectrum, contact and stomach action with some ovicidal activity, acts by inhibiting oxidative phosphorylation. Diafenthiuron, is a potent in vitro inhibitor of mitochondrial respiration in rat liver and Calliphora flight muscles. It selectively blocks the coupling site in a time-dependent manner which is different from the action of other pesticides. The resemblance in the chemical structure to the well-known mitochondrial inhibitor dicyclohexylcarbodiimide suggests that CGA 140408 inhibits mitochondrial ATPase.

Tolfenpyrad:
Tolfenpyrad is an aromatic amide obtained by formal condensation of the carboxy group of 4-chloro-3-ethyl-l-methylpyrazole-5-carboxylic acid with the amino group of l-[4-(4-methylphenoxy) phenyl] methylamine. It has a role as a mitochondrial NADH: ubiquinone reductase inhibitor, an agrochemical, an EC 1.3.5.1 [succinate dehydrogenase (quinone)] inhibitor and an antifungal agent. It is a pyrazole insecticide, an aromatic amide, an aromatic ether and an organochlorine compound.
It has an IUPAC name as 4-chloro-5-ethyl-2-methyl-N-[[4-(4-methylphenoxy) phenyl] methyl] pyrazole-3-carboxamide and has a chemical structure as follows:

Tolfenpyrad is a novel pyrazole heterocyclic efficient pesticides. Its mechanism of action is passed on the complex I of system for the electronics that hinders in the mitochondrial metabolic system, is hindered thereby electronics is passed on, and insect can not be provided and storing energy, is called as the mitochondria electronics and passes on complex Inhibitors (METI).This medicament insecticidal spectrum is wide, to insect and the equal special efficacy of mite class such as various Lepidopteras, thrips, coleoptera, Homoptera, diptera, Semipteras. Tolfenpyrad is a contact insecticide that inhibits an organism's energy metabolism and can also acts as a contact fungicide. This medicament lasting period is longer, to the insect whole growing, higher activity is arranged from the ovum to the adult, and the antagonism insect also produces effect. In addition, it is also big that this medicament is used the crop scope, and it can be applicable to the control of insect of various crop such as crops, vegetables, fruit tree, flowers, tealeaves.
Flonicamid:
Flonicamid is a pyridinecarboxamide that is nicotinamide substituted by a trifluoromethyl group at position 4 and a cyanomethyl group at the carbamoyl nitrogen

atom. It has a role as a xenobiotic, an environmental contaminant and an insecticide. It is a pyridinecarboxamide, a nitrile and an organofluorine compound. It derives from a nicotinamide.
It has an IUPAC name asN-(cyanomethyl)-4-(trifluoromethyl)pyridine-3-carboxamide having chemical structure as follows:
O CF3
N^ H
Flonicamid rapidly inhibits the feeding behavior of aphids, and has better action through ingestion than by contact. Its mode of action is different from other insecticides such as neonicotinoids, pymetrozine and pyri-uquinazon. Flonicamid belongs to IRAC Group 29 and is the only insecticide in this class. Flonicamid also has a good toxicological, environmental and ecotoxicological profile.
The present inventors believe that the oil-based agrochemical composition of the present invention surprisingly results in a synergistic action. The compsiotion of the present invention allow for a broad spectrum of pest insect-control and has surprisingly improved plant vigour and yield. The broad spectrum of the present composition also provides a solution for preventing the development of resistance.
The oil-based agrochemical composition has very advantageous curative, preventive insecticidal properties for protecting cultivated plants. As has been mentioned, said active ingredients composition can be used to inhibit or destroy the insect-pest and mites that occur on plants or parts of plants (fruit, blossoms, leaves, stems, tubers, roots) of different crops or useful plants, while at the same time those parts of plants which grow later are also protected from attack by such insect-pest and mites. Active ingredients composition has the special advantage of being highly active against insect-pests in the soil that mostly occur in the early stages of plant development.

The oil-based agrochemical composition comprising of bioactive amounts of a) Diafenthiuron; b) an insecticide selected from Tolfenpyrad or Flonicamid in the form of oil dispersion formulation is used to protect the crops and plants from insect and pests. The lists of the major crops includes but are not limited to GMO (Genetically Modified Organism) and Non GMO varieties of Cotton (Gossypium spp.), Paddy (Oryza sativa), Wheat (Triticum aestavum), Barley (Hordeum vulgare), Maize (Zea mays), Sorghum (Sorghum bicolor), Oat (Avena sativa), Pearl millet (Pennisetum glaucum), Sugarcane (Saccharum officinarum) , Sugarbeet (Beta vulgaris), Soybean (Glycin max), Peanut (Arachis hypogaea), Sunflower (Helianthus annuus) , Mustard (Brassica juncea), Rape seed (Brassica napus), Linseed (Linum usitatissimum), Sesame (Sesamum indicum), Green gram (Vigna radiata), Black gram (Vigna mungo), Chickpea (Cicer aritinum), Cowpea (Vigna unguiculata), Redgram (Cajanus cajan), Frenchbean (Phaseolus vulgaris), Indian bean (Lablab purpureus), Horse gram (Macrotyloma uniflorum), Field pea (Pisum sativum), Cluster bean (Cyamopsis tetragonoloba), Lentils (Lens culinaris), Brinjal (Solanum melongena), Cabbage (Brassica oleracea var. capitata), Cauliflower (Brassica oleracea var. botrytis), Okra (Abelmoschus esculentus) , Onion (Allium cepa L.), Tomato (Solanum lycopersicun) , Potato (Solanum tuberosum) , Sweet potato (Ipomoea batatas), Chilly (Capsicum annum), Garlic (Allium sativum), Cucumber (Cucumis sativus), Muskmelons (Cucumis melo), Watermelon (Citrullus lanatus), Bottle gourd (Lagenaria siceraria), Bitter gourd (Momordica charantia), Radish (Raphanus sativus), Carrot (Dacus carota subsp. sativus), Turnip (Brassica rapa subsp rapa), Apple (Melus domestica), Banana (Musa spp.), Citrus groups (Citrus spp.), Grape (Vitis vinifera), Guava (Psidium guajava), Litchi (Litchi chinensis), Mango (Mangifera indica), Papaya (Carica papaya), Pineapple (Ananas comosus), Pomegranate (Punica granatum) , Sapota (Manilkara zapota), Tea (Camellia sinensis), Coffea (Coffea Arabica), Turmeric (Curcuma longa), Ginger (Zingiber officinale), Cumin (Cuminum cyminum), Fenugreek (Trigonella foenum-graecum), Fennel (Foeniculum vulgare), Coriander (Coriandrum sativum), Ajwain (Trachyspermum ammi), Psyllium (Plantago ovate), Black Pepper (Piper nigrum), Stevia (Stevia rebaudiana), Safed musli (Chlorophytum tuberosum), Drum stick (Moringa oleifera), Coconut (Coco nucifera), Mentha (Mentha spp.), Rose (Rosa spp.), Jasmine (Jasminum spp.), Marigold ( Tagetes spp.), Common daisy (Bellis perennis), Dahlia (Dahlia hortnesis), Gerbera ( Gerbera jamesonii), Carnation (Dianthus caryophyllus), vegetables: solanaceous vegetables such as eggplant, tomato,

pimento, pepper, potato, etc., cucurbit vegetables such as cucumber, pumpkin, zucchini, water melon, melon, squash, etc., cruciferous vegetables such as radish, white turnip, horseradish, kohlrabi, Chinese cabbage, cabbage, leaf mustard, broccoli, cauliflower, etc., asteraceous vegetables such as burdock, crown daisy, artichoke, lettuce, etc, liliaceous vegetables such as green onion, onion, garlic, and asparagus, ammiaceous vegetables such as carrot, parsley, celery, parsnip, etc., chenopodiaceous vegetables such as spinach, Swiss chard, etc., lamiaceous vegetables such as Perilla frutescens, mint, basil, etc, strawberry, sweet potato, Dioscorea japonica, colocasia, etc., flowers, foliage plants, turf grasses, fruits: pome fruits such apple, pear, quince, etc, stone fleshy fruits such as peach, plum, nectarine, Prunus mume, cherry fruit, apricot, prune, etc., citrus fruits such as orange, lemon, rime, grapefruit, etc., nuts such as chestnuts, walnuts, hazelnuts, almond, pistachio, cashew nuts, macadamia nuts, etc. berries such as blueberry, cranberry, blackberry, raspberry, etc., grape, kaki fruit, olive, plum, banana, coffee, date palm, coconuts, etc. , trees other than fruit trees; tea, mulberry, flowering plant, trees such as ash, birch, dogwood, Eucalyptus, Ginkgo biloba, lilac, maple, Quercus, poplar, Judas tree, Liquidambar formosana, plane tree, zelkova, Japanese arborvitae, fir wood, hemlock, juniper, Pinus, Picea, and Taxus cuspidate, etc.
The oil-based agrochemical composition of the present invention is used to control the insects-pests. The major insects pests are belongs to the order Hemiptera, for example, rice leafhopper/green leaf hopper (GLH) Nephotettix nigropictus, rice brown plant hopper (BPH) Nilaparvata lugen, rice backed plant hopper (WBPH) Sogatella furcifera, Apple Mealy bug Phenococcus aceris, bean aphid Aphis fabae, black citrus aphid Toxoptera aurantii, citrus black scale Saissetia oleae, cabbage aphid Brevicoryne brassicae, Lipaphis erysimi, citrus red scale Aonidiella aurantii, yellow scale Aonidiella citrine, citrus mealybug Planococcus citri, corn leaf aphid Rhopalosiphum maidis, aphid Aphis gossypii, jassid Amrasca biguttula biguttla, mealy bug Planococcus spp. And Pseudococcus spp., cotton stainer Dysdercus suturellus, whitefly Bemisia tabaci, cowpea aphid Aphis crassivora, grain aphid Sitobion avenae, golden glow aphid Uroleucon spp., grape mealybug Pseudococcus maritimus, green peach aphid Myzus persicae, greenhouse whitefly Trialeurodes vaporariorum, papaya mealy bug Pracoccus marginatus, pea aphid Acyrthosiphon pi sum, sugarcane mealybug Saccharicoccus sacchari, potato aphid Myzus persicae, potato leaf hopper Empoasca fabae, cotton whitefly Bemisia tabaci, tarnished plant bug Lygus lineolaris, wooly apple aphid

Eriosoma lanigerum, mango hopper Amritodus atkinsoni, Idioscopus spp. ; order Lepidoptera, army worm Mythimna unipuncta, asiatic rice borer Chilo suppressalis, bean pod borer Maruca vitrata, beet armyworm Spodoptera exigua, black cutworm Agrotis ipsilon, bollworm Helicoverpa armigera , cabbage looper Trichoplusia ni, codling moth Cydia pomonella, croton caterpillar Achea Janata, diamond backmoth Plutella xylostella, cabbage worm Pieris rapae, pink bollworm Pectinophora gossypiella, sugarcane borer Diatraea saccharalis, sugarcane early shoot borer Chilo infuscatellus tobacco budworm Heliothis virescens, tomato fruitworm Helicoverpa zea, velvet bean caterpillar Anticarsia gemmatalis, yellow stem borer Scirpophaga incertulas, spotted bollworm Earias vittella, rice leaffolder Cnaphalocrocis medinalis, pink stem borer Sesamia spp., tobacco leafeating caterpillar Spodoptera litura; brinjal fruit and shoot borer Leucinodes orbonalis, bean pod borer Maruca vitrata, Maruca testulalis, armyworm Mythimna separata, cotton pinkbollworm Pectinophora gossypiella, citrus leafminer Phyllocnistis citrella, cabbage butterfly Pieris bras-sicae, diamond backmoth Plutella xylostella, paddy stem borer Scirpophaga excerptallis, Scirpophaga incertulas, Scirpophaga innotata, wheat stem borer Sesamia inferens, Sitotroga cerealella, Spilosoma obliqua, fall armyworm Spodoptera frugiperda, Spodoptera littoralis, Spodoptera litura, Trichoplusia ni, Tryporyza nivella, Tryporyza incertulas, Tuta absoluta.
from the order Coleoptera, for example, apple twig borer Amphicerus spp., corn root worm Diabrotica virgifera, cucumber beetle diabrotica balteata, boll weevil Anthonomus grandis, grape flea beetle Altica chalybea, grape root worm Fidia viticola, grape trunk borer Clytoleptus albofasciatus, radish flea beetle Phyllotreta armoraciae, maize weevil Sitophilus zeamais, northern corn rootworm Diabrotica barberi, rice water weevil Lissorhoptrus oryzophilus, Anthonomus grandis, Bruchus lentis, Diabrotica semipunctata, Diabrotica virgifera, Dicladispa armigera, Epila-chna varivestis, various species of white grubs are Holotrichia bicolor, Holotrichia consanguinea, Holotrichia serrata, Leptinotarsa decemlineata, Phyllotreta chrysocephala, Popillia japonica etc; from the order Orthoptera, for example, Gryllotalpa spp., Locusta spp., and Schistocerca is spp.; from the order Thysanoptera, for example, Thrips- Frankliniella spp., Thrips palmi, Thrips tabaci and Scirtothrips dorsalis; termites (Isoptera), e.g. Calotermes flavicollis, Coptotermes formosanus, Heterotermes aureus, Leucotermes flavipes, Microtermes obesi, Odontotermes obesus, Reticulitermes flavipes, Termes

natalensis; from the order Heteroptera, for example, Dysdercus spp., Leptocorisa spp., from the order Hymenoptera, for example, Solenopsis spp.; from the order Diptera, for example, Antherigona soccata, Dacus spp., Liriomyza spp., Melanagromyza spp., from the order Acarina, for example, Aceria mangiferae, Brevipalpus spp., Eriophyes spp., Oligonychus mangiferus, Oligonychus punicae, Panonychus citri, Panonychus ulmi, Polyphagotarsonemus latus, Tarsonemus spp., Tetranychus urticae, Tetranychus cinnabarinus.
The oil dispersion formulation according to the invention can be applied to any and all developmental stages of pests, such as egg, larva, pupa, and adult. The pests may be controlled by contacting the target pest, its food supply, habitat, breeding ground or its locus with a pestici dally effective amount of the inventive mixtures or of compositions comprising the mixtures.
The term "health of a plant" or "plant health" is defined as a condition of the plant and/or its products. As a result of the improved health, yield, plant vigor, quality and tolerance to abiotic or biotic stress are increased. Noteworthy, the health of a plant when applying the method according to the invention, is increased independently of the pesticidal properties of the active ingredients used because the increase in health is not based upon the reduced disease pressure but instead on complex physiological and metabolic reactions which result for example in an activation of the plant's own natural defense system. As a result, the health of a plant is increased even in the absence of diseases pressure. Accordingly, in an especially preferred embodiment of the method according to the invention, the health of a plant is increased both in the presence and absence of biotic or abiotic stress factors. The above identified indicators for the health condition of a plant may be interdependent or they may result from each other. An increase in plant vigor may for example result in an increased yield and/or tolerance to abiotic or biotic stress. One indicator for the condition of the plant is the yield. "Yield" is to be understood as any plant product of economic value that is produced by the plant such as grains, fruits in the proper sense, vegetables, nuts, grains, seeds, wood (e.g. in the case of silviculture plants) or even flowers (e.g. in the case of gardening plants, ornamentals). The plant products may in addition be further utilized and/or processed after harvesting.

In an especially preferred embodiment of the invention, the yield of the treated plant is increased.
In another preferred embodiment of the invention, the yield of the plants treated according to the method of the invention, is increased synergistically.
According to the present invention, "increased yield" of a plant, in particular of an agricultural, silvi cultural and/or horticultural plant means that the yield of a product of the respective plant is increased by a measurable amount over the yield of the same product of the plant produced under the same conditions, but without the application of the mixture according to the invention.
Increased yield can be characterized, among others, by the following improved proper¬ties of the plant: increased plant, weight, increased plant height, increased biomass such as higher overall fresh weight (FW), increased number of flowers per plant, higher grain yield, more tillers or side shoots (branches), larger leaves, increased shoot growth, increased protein content, increased oil content, increased starch content, increased pigment content, increased leaf are index.
According to the present invention, the yield is increased by at least 5 %, preferable by 5 to 10 %, more preferable by 10 to 20 %, or even 20 to 30 % compared to the untreated control plants or plants treated with pesticides in a way different from the method according to the present invention. In general, the yield increase may even be higher.
One or more active ingredients is formulated using vegetables oil for various purpose, such as to increase the biological activity by increasing penetration, spreading and retention, or to obtain a physical or chemically stable oil-based formulation.
Further in present Oil Dispersion formulation having vegetable oils as carrier increases penetration into leaf surfaces, especially plant, improves retention of active ingredients on leaf surfaces, increases spreading action, so faster spray coverage on leaf surface and easily dissolve the waxy layer of the leaf surface.
Oil dispersions already contain in their composition oil, such as a mineral or vegetable oil, and emulsifiers, which can act as penetration adjuvants when applied in the field.
Oil Dispersion formulations can be deemed "adjuvanted" formulations and do not require additional associated adjuvants to be applied in the field.

In comparison to other formulations oil content in the Oil Dispersion formulations gives a favorable eco-toxicological profile guarantying a very high biological efficacy. Further ODs are non-toxic and non-flammable formulations with very high biological efficacy.
Oil Dispersion formulations due to oil content give inherent technical solution over formulation containing water sensitive active ingredients.
Oil Dispersion formulations are having economic significance as well. Most of the formulations has more cost of preparation due to addition of adjuvants. OD, with its oil content and better particle size distribution, and no additional adjuvant required herey combine high efficacy with better cost.
Further the said oil-based agrochemical composition comprises of bioactive amounts of (A) an insecticide Diafenthiuron; (B) an insecticide selected from Tolfenpyrad or Flonicamid and are present in the said composition in specific fixed ratio. In further aspect the present invention relates to the oil-based agrochemical composition comprising bioactive amounts of (A) is 1.0 to 50% w/w of the composition; and (B) is 1.0 to 50% w/w of the composition.
More particularly in further aspect of the present invention provides the oil-based agrochemical composition comprising bioactive amounts of diafenthiuron (1 to 50%) and tolfenpyrad (1 to 50%); or diafenthiuron (1 to 50%) and flonicamid (1 to 50%).

Active Ingredients Compound A Compound B
Examples Diafenthiuron Tolfenpyrad or Flonicamid
% of Active Ingredient 1.0 to 50% 1.0 to 50%
The process for preparing the present synergistic mixture can be modified accordingly by any person skilled in the art based on the knowledge of manufacturing the formulation. However all such variations and modifications are covered by the scope of the present invention.

The composition of the present invention in addition to bioactive amounts of active ingredients further comprises inactive excipients including but not limited to wetting agent, dispersant or dispersing agent, anti-freezing agent, anti-foam agent, solvent, co-solvents, preservatives, stabilizers.
A wetting agent is a substance that when added to a liquid increases the spreading or penetration power of the liquid by reducing the interfacial tension between the liquid and the surface on which it is spreading. Wetting agents are used for two main functions in agrochemical formulations: during processing and manufacture to increase the rate of wetting of powders in water to make concentrates for soluble liquids or suspension concentrates; and during mixing of a product with water in a spray tank or other vessel to reduce the wetting time of wettable powders and to improve the penetration of water into water-dispersible granules.
Examples of wetting agent used herein for Oil dispersion (OD) formulation includes but not limited Organosilicone surfactants includes trisiloxane ethoxylate, polydimethylsiloxane, polyoxyethylene methyl polysiloxane, polyoxyalkylene methyl polysiloxane, polyether polymethyl siloxane copolymer, heptamethyl trisiloxane, Polyalkyleneoxide modified heptamethyl trisiloxane, polyether modified polysiloxane, may or may not be in modified form, may be liquid or powder form or mixture thereof etc.
A dispersant or a dispersing agent is a substance which adsorbs onto the surface of particles and helps to preserve the state of dispersion of the particles and prevents them from re-aggregating. Dispersants are added to agrochemical formulations to facilitate dispersion and suspension during manufacture, and to ensure the particles re-disperse into water in a spray tank. They are widely used in wettable powders, suspension concentrates and water-dispersible granules. Surfactants that are used as dispersants have the ability to adsorb strongly onto a particle surface and provide a charged or steric barrier to re-aggregation of particles. The most commonly used surfactants are anionic, non-ionic, or mixtures of the two types. For wettable powder formulations, the most common dispersants are sodium lingo sulphonates. In recent years, new types of very high molecular weight polymeric surfactants have been developed as dispersants. These have very long hydrophobic 'backbones' and a large number of ethylene oxide chains forming the 'teeth' of a 'comb' surfactant. These high molecular weight polymers can

give very good long-term stability to suspension concentrates because the hydrophobic backbones have many anchoring points onto the particle surfaces.
Examples of dispersants or dispersing agent used herein for Oil dispersion (OD) formulation includes but not limited to alkyl sulfonates, alkyl benzene sulfonates, alkyl aryl sulfonates, alkylphenolalkoxylates, tristyrylphenol ethoxylates, natural or synthetic fatty ethoxylate alcohols, natural or synthetic fatty acid alkoxylates, natural or synthetic fatty alcohols alkoxylates, alkoxylated alcohols (such as n-butyl alcohol poly glycol ether), block copolymers (such as ethylene oxide-propylene oxide block copolymers and ethylene oxide-butyl ene oxide block copolymers), fatty acid-polyalkylene glycol condensates, polyamine-fatty acid condensates, polyester condensates, salts of polyolefin condensates, sodium ligno sulfonate, sodium ploycarboxylate, EO/PO based copolymer, phenol sulfonate, sodium methyl oleoyl taurate, styrene acrylic acid copolymer, propyleneoxide-ethyleneoxide-copolymer, polyethylene glycol 2,4,6-tristyrylphenyl ether, tristyrylphenol-polyglycolether-phosphate, tristyrylphenole with 16 moles EO, tristyrylphenol-polyglycolether-phosphate, oleyl-polyglycolether with ethylene oxide, tallow fattyamine polyethylene oxide, nonylphenol polyglycolether with 9-10 moles ethylene oxide.
Emulsifying agent used herein for the Oil dispersion (OD) formulation includes but not limited to castor oil ethoxylates, alcohol ethoxylates, fatty acid ethoxylates, sorbitan ester ethoxylates, sulphosuccinate, calcium salts of dodecylbenzene sulphonate, alkylammonium salts of alkylbenzene sulphonate, alkyl sulphosuccinate salts, ethylene oxide-propylene oxide block copolymers, ethoxylated alkylamines, ethoxylated alkyl phenols, polyoxyethylene sorbitan monolaurate etc.
Stabilizers or stabilizing agent used herein for the Oil dispersion (OD) formulation includes but not limited to hectorite clay, aluminum magnesium silicate, bentonite clay, silica, attapulgite clay.
Antifoaming agent used herein for the Oil dispersion (OD) formulation includes but not limited to silicone oil, silicone compound, C10~C20 saturated fat acid compounds or C8~C10 aliphatic alcohols compound, silicone antifoam emulsion, dimethylsiloxane, polydimethyl siloxane, vegetable oil based antifoam, tallow based fatty acids, polyalkyleneoxide modified polydimethylsiloxane etc.

Anti-freezing agent used herein for the Oil dispersion (OD) formulation includes but not limited to ethylene glycol, propane diols, glycerine or the urea, glycol (Monoethylene glycol, Diethylene glycol, Polypropylene glycol, Polyethylene glycol), glycerine, urea, magnesium sulfate heptahydrate, sodium chloride etc;
Preservative used herein for the Oil dispersion (OD) formulation includes but not limited to l,2-benzisothiazolin-3(2H)-one, sodium salt, sodium benzoate, 2-bromo-2-nitropropane-l,3-diol, formaldehyde, sodium o-phenylphenate, 5-chloro-2-methyl-4-isothiazolin-3-one & 2-methyl-4-isothiazolin-3-one.
Solubilizer used herein for the Oil dispersion (OD) formulation includes but not limited to Polyvinyl pyrrolidone.
Solvent used herein for the Oil dispersion (OD) formulation includes but not limited to vegetable oil (plant, seed or tree) or its alkylated or ethoxylated or esterified. The alkylated vegetable oil may be methylated vegetable oil or ethylated vegetable oil. The vegetable oils include olive oil, kapok oil, castor oil, papaya oil, camellia oil, sesame oil, corn oil, rice bran oil, cotton seed oil, soybean oil, groundnut oil, rapeseed-mustard oil, linseed oil, tung oil, sunflower oil, safflower oil, coconut oil. The alkyl ester of vegetable oils includes methyl ester, ethyl ester, propyl ester or butyl ester of vegetable oils. Some of the examples are methylated seed oil, polyalkyleneoxide modified polydimethylsiloxane alkylphenol ethoxylate, rapeseed oil methyl ester, rapeseed oil ethyl ester, rapeseed oil propyl esters, rapeseed oil butyl esters, soybean oil methyl ester, soybean oil ethyl ester, soybean oil propyl ester, soybean oil butyl ester, castor oil methyl ester, castor oil ethyl ester, castor oil propyl ester, castor oil butyl ester, cotton seed oil methyl ester, cotton seed oil ethyl ester, cotton seed oil butyl ester, cotton seed oil propyl ester, tall oil fatty acids esters-tallow methyl ester, tallow ethyl ester, tallow propyl ester, bio-diesel, mineral oil (aromatic solvents, isoparaffin, base solvent), fatty acid amides (e.g. CI -C3 amines, alkylamines or alkanolamines with C6-C18 carboxylic acids), fatty acids, alkyl esters of fatty acids, methyl and ethyl oleate, methyl and ethyl soyate, alkyl benzenes and alkylnaphthalenes, polyalkylene glycol ethers, fatty acid diesters, fatty alkylamides and diamides, dialkylene carbonates, ketones and alcohols. The above oil based carrier/diluting agents may be used as solo or mixture of two or more if desired. All the said oils used as a carrier or diluent are procured from the vendor based in Gujarat State.

Cosolvent used herein for the Oil dispersion (OD) formulation in addition to formulation excipients includes but not limited to Cyclohexanone, Acetophenone, NMP, Dimethyl sulfoxide, Benzyl alcohol, Butanol, N-octanol, N-Propanol, 2-ethyl hexanol, Tetrahydro furfuryl alcohol, Isophorone, Fatty acid dimethyl amide, 2-hexylethyl lactate, Propylene carbonate.
While the foregoing written description of the invention enables one of ordinary skill to make and use what is considered presently to be the best mode thereof, those of ordinary skill will understand and appreciate the existence of variations, combinations, and equivalents of the specific embodiment, method, and examples herein. The invention should therefore not be limited by the above described embodiment, method, and examples, but by all embodiments and methods within the scope and spirit of the invention. The invention shall now be described with reference to the following specific examples. It should be noted that the example(s) appended below illustrate rather than limit the invention, and that those skilled in the art will be able to design many alternative embodiments without departing from the scope of the present invention.
These and other aspects of the invention may become more apparent from the examples set forth herein below. These examples are provided merely as illustrations of the invention and are not intended to be construed as a limitation thereof.
EXAMPLE 1:
OD (Oil Dispersion) formulation of Diafenthiuron 16%+Tolfenpyrad 6%

Composition content %
(w/w)
Diafenthiuron a.i. 16.00
Tolfenpyrad a.i. 6.00
Dispersing agent Tri styrylphenol -polyglycol ether-phosphate 4.50
Emulsifying agent Alcohol ethoxylate 8.00
Solubilizer Polyvinyl pyrrolidone 2.00
Stabilizer Bentonite clay 1.50
Antifoaming agent Polydimethyl siloxane 0.30
Preservative l,2-benzisothiazolin-3(2H)-one 0.20
Antifreezing agent Polypropylene glycol 5.00
Wetting agent Trisiloxane ethoxylate 5.00
Carrier as solvent Methylated seed oil 51.50
Total 100.00

Storage stability- Diafenthiuron 16%+Tolfenpyrad 6% OD (Oil Dispersion) formulation
Storage stability study in laboratory (at 54+2 C & At 0+2 C temp, for 14 days) and at room temperature (for 12 months) shows that Diafenthiuron 16%+Tolfenpyrad 6% OD (Oil Dispersion) formulation complies all the in-house parameters like active ingredients content, suspensibility, wettability, wet sieve percent by mass, pH range and moisture content.
Procedure: Manufacturing process of Oil dispersion (OD) formulation:

Preparation of Oil dispersion (OD) formulation:
Part A Preparation of the liquid premix
Step 1 Charge Vegetable oil or solvent or both into a vessel with anchor stirrer.
Step 2 Under stirring, add the emulsifier and dispersing agent and stir until all ingredients are dissolved completely.
PartB Preparation of the slurry
Step 1 Now, charge the liquid premix into a second vessel, equipped with a cooling and heating device and a high shear stirrer.
Step 2 Add the active ingredient and homogenize thoroughly. Pre-mill this mixture and finally mill it using a bead mill to achieve a particle size distribution as required by the specification.
PartC Preparation of the Thickener gel
Step 1 Charge the vegetable/plant/seed oil or solvent to the vessel, equipped with a high shear stirrer.
Step 2 Add gradually the thickener which is organophilic clay, maintaining high-shear mixing throughout. Stirring is continued until thoroughly mixed.
Step 3 Under stirring, the thickener activating agent propylene carbonate is added. Allow the gel to swell whilst maintaining mixing.
PartD Preparation of the final formulation
Step 1 Now add the thickener gel or silica and disperse the mixture by using a high shear stirrer.

Step 2 Finally add the recommended wetting and spreading agent or adjuvants (silicone or non-silicone based) to this formulation and disperse by using high shear stirrer.
Step 3 Check the finished formulation to specification.
Step 4 After approval, material is packed in required pack sizes.
EXAMPLE 2:
OD (Oil Dispersion) formulation of Diafenthiuron 16%+Flonicamid 4%

Composition content
%
(w/w)
Diafenthiuron a.i. 16.00
Flonicamid a.i. 4.00
Tristyrylphenol-polyglycolether-phosphate 4.50
Alcohol ethoxylate 8.00
Polyvinyl pyrrolidone 2.00
Bentonite clay 1.50
Polydimethyl siloxane 0.30
l,2-benzisothiazolin-3(2H)-one 0.20
Polypropylene glycol 5.00
Trisiloxane ethoxylate 5.00
Methylated seed oil 53.50
Total 100.00
Storage stability- Diafenthiuron 16%+Flonicamid 4% OD (Oil Dispersion) formulation
Storage stability study in laboratory (at 54+2 C & At 0+2 C temp, for 14 days) and at room temperature (for 12 months) shows that Diafenthiuron 16%+Flonicamid 4% OD (Oil Dispersion) formulation complies all the in-house parameters like active ingredients content, suspensibility, wettability, wet sieve percent by mass, pH range and moisture content.
Procedure: Manufacturing process of Oil dispersion (OD) formulation:
Manufacturing process for the Oil dispersion (OD) formulation is as per Example 1.

Prior art formulation
Diafenthiuron 16%+Tolfenpyrad 6% SC

Composition content %
(w/w)
Diafenthiuron a.i. 16.00
Tolfenpyrad a.i. 6.00
Dispersing agent Tristryphenole with 16 moles EO 4.50
Stabilizer Bentonite clay 1.50
Antifoaming agent Polydimethyl siloxane 0.30
Preservative 1,2-benzi sothi azolin-3 (2H)-one 0.20
Antifreezing agent Polypropylene glycol 5.00
Wetting agent EO/PO block copolymer 5.00
Thickner Xanthan gum 0.20
Carrier as solvent Water 57.30
Total 100.00
Prior art formulation
Diafenthiuron 16%+Flonicamid 4% SC

Composition content % (w/w)
Diafenthiuron a.i. 16.00
Flonicamid a.i. 4.00
Tristryphenole with 16 moles EO 4.50
Bentonite clay 1.50
Polydimethyl siloxane 0.30
1,2-benzi sothi azolin-3 (2H)-one 0.20
Polypropylene glycol 5.00
EO/PO block copolymer 5.00
Xanthan gum 0.20
Water 59.30
Total 100.00
Prior art formulation
Diafenthiuron 32%+Tolfenpyrad 12% WG

Composition content %
(w/w)
Diafenthiuron a.i. 32.00
Flonicamid a.i. 12.00
Alkylated naphthalene sulfonate, sodium salt 7.00

5.00
Polyacrylate polymer sodium salt

Sodium alkyl naphthalene sulfonate blend 3.50
Silicone antifoam 1.00
Sodium sulfate anhydrous 10.00
Corn starch 5.00
China Clay 24.50
Total 100.00

Prior art formulation
Diafenthiuron 32%+Flonicamid 8% WG

Composition content %
(w/w)
Diafenthiuron a.i. 32.00
Flonicamid a.i. 8.00
Alkylated naphthalene sulfonate, sodium salt 7.00
Polyacrylate polymer sodium salt 5.00
Sodium alkyl naphthalene sulfonate blend 3.50
Silicone antifoam 1.00
Sodium sulfate anhydrous 10.00
Corn starch 5.00
China Clay 28.50
Total 100.00
Prior art formulation
Diafenthiuron 16%+Tolfenpyrad 6% SE

Composition content %
(w/w)
Diafenthiuron a.i. 16.00
Tolfenpyrad a.i. 6.00
Dispersing agent Acrylic graft copolymer 5.00
Solvent C9-10 aromatic solvent 15.00
Emulsifying agent Alcohol ethoxylate 5.00
Stabilizer Bentonite clay 1.50
Antifoaming agent Polydimethyl siloxane 0.30
Preservative l,2-benzisothiazolin-3(2H)-one 0.20
Antifreezing agent Polypropylene glycol 5.00
Thickner Xanthan gum 0.20
Wetting agent EO/PO block copolymer 5.00
Carrier as solvent Water 36.80
Total 100.00

Biological Examples:
A synergistic effect exists wherever the action of a combination of active ingredient is greater than the sum of the action of each of the components alone. Therefore, a synergistically effective amount or an effective amount of a synergistic composition or combination is an amount that exhibits greater pesticidal activity than the sum of the pesticidal activities of the individual components.
In the field of agriculture, it is often understood that the term "synergy" is as defined by Colby S.R. in an article entitled " Calculation of the synergistic and antagonistic responses of herbicide combinations" published in the journal Weeds, 1967, 15, p.20-22, incorporated herein by reference in its entirety. The action expected for a given combination of two active components can be calculated as follows:
Colby's formula for calculating synergism between two active ingredients
XY
E = X + Y -
100
Where, E= Expected % control by mixture/combination of Compound A and Compound B in a defined dos
X= Observed % control by Compound A Y= Observed % control by Compound B
Observed value (% control)
Ratio =
Expected value (% control)
Ratio of O/E > 1, means synergism observed
FIELD BIO-EFFICACY STUDIES:
The field trials have been carried out on different crops to judge the synergism and benefits of OD (oil dispersion) formulation of diafenthiuron+tolfenpyrad and di afenthiuron+fl oni cami d. Experiment 1: Control of thirps, Scirtothirps dorsalis infesting chilli crop
Crop : Chilli
Location : Umreth, Gujarat
Plot size : 25 sq.mt.
Number of Treatments: 8 including untreated check.
Crop Stage : 73 DAS (Days after transplanting)
Spray volume : 480 liter/h
Method of Application: Foliar spray with battery operated knap sack sprayer fitted
with hollowcone nozzle.
Agronomic Practices : All agronomic practices followed as per the crop requirement
except insect control.

Observation Methods:
Thrips (Scirtothrips dorsalis) control : Count the number of live thrips by shaking the twigs gently on black color paper piece. Record the observations from 3 twigs per plant and 10 plants per plot on before spray, 1,3,7, 10 and 14 DAA (Days after application). Calculate % thrips control (as observed value) and apply Colby's formula (given above) to calculate expected control and thereby to judge the synergism.
Number of live Thrips in treated plot
% Thrips control = 100 x 100
Number of live Thrips in untreated (UTC) plot
Fruit count: Count the number of healthy fruits per plant. Record the observations from 10 plants per plot on 14th day after application.
Table l:Treatment details

Treatment Number Treatment details Use rate (ml/h) Use rate (g.a.i./h)
Tl Diafenthiuron 16%+Tolfenpyrad 6% OD 1250 ml 200+75
T2 Diafenthiuron 16%+Tolfenpyrad 6% SC 1250 ml 200+75
T3 Diafenthiuron 16%+Tolfenpyrad 6% SE 1250 ml 200+75
T4 Diafenthiuron 32%+Tolfenpyrad 12% WG 625 g 200+75
T5 Diafenthiuron 50% WP+Tolfenpyrad 15% EC (tank mix) 400 g+500 ml 200+75
T6 Diafenthiuron 50% WP 400 g 200
T7 Tolfenpyradl5%EC 500 ml 75
T8 UTC (Untreated Check) 0 0
g.a.i.- gram active ingredient, OD oil dispersion, SC suspension concentrate, SE suspo
emulsion, WG wettable granule, WP wettable powder, EC emulsifiable concentrate.
Table 2a: Residual control of chilli thrips

Treatment Number Chilli Thrips, Scirtot irips dorsalis control

Observed control (%) at Expected control (%) at

1 DAA 3 DAA 7 DAA 10 DAA 14 DAA 1 DAA 3 DAA 7 DAA 10 DAA 14 DAA
Tl 98.20 93.40 85.20 74.60 61.20 88.69 85.99 80.29 71.53 59.93
T2 97.20 90.80 80.60 68.40 54.40 88.69 85.99 80.29 71.53 59.93
T3 96.80 89.20 79.20 65.60 51.60 88.69 85.99 80.29 71.53 59.93
T4 96.40 88.40 77.80 62.80 47.40 88.69 85.99 80.29 71.53 59.93
T5 95.80 86.60 76.60 60.20 45.40 88.69 85.99 80.29 71.53 59.93
T6 68.40 64.80 58.60 50.40 42.60
T7 64.20 60.20 52.40 42.60 30.20
T8 0.00 0.00 0.00 0.00 0.00
DAA days after application
Table 2b :Control of chilli thrips

Treatment Number Colby's ratio Synergism observed (Yes / No)

1 DAA 3 DAA 7 DAA 10 DAA 14 DAA 1 DAA 3 DAA 7 DAA 10 DAA 14 DAA
Tl 1.11 1.09 1.06 1.04 1.02 Y Y Y Y Y
T2 1.10 1.06 1.00 0.96 0.91 Y Y N N N
T3 1.09 1.04 0.99 0.92 0.86 Y Y N N N
T4 1.09 1.03 0.97 0.88 0.79 Y Y N N N
T5 1.08 1.01 0.95 0.84 0.76 Y Y N N N
T6
T7
T8
The field trial results shows that Tl (Diafenthiuron 16%+Tolfenpyrad 6% OD) provides synergistic control of chilli thrips up to 14 days after application where as all other conventional treatments (T2, T3, T4 and T5) provides synergistic control of chilli thrips upto 3 DAA only. The level of control (means percentage) was also observed higher in Tl in comparison with all other conventional treatments (T2, T3, T4 and T5).
Table 3: Fruit count

Treatment Number Number of chilli fruits/plant at 14 DAA % Increase in number of fruits over T8 % Increase in number of fruits in Tl over

T2 T3 T4 T5
Tl 48.5 47.9 6.8 9.7 10.7 15.8
T2 45.4 38.4
T3 44.2 34.8
T4 43.8 33.5
T5 41.9 27.7
T6 35.7 8.8
T7 36.5 11.3
T8 32.8 0.0
Tl (Diafenthiuron 16%+Tolfenpyrad 6% OD) also produces higher number of green chilli fruits as compared to all conventional treatments (T2, T3, T4 and T5). Experiment 2: Control of whitefly, Bemisia tabaci in cotton
Crop : Cotton
Location : Karjan, Gujarat
Plot size : 40 sq. mt. (8m x 5m)
Number of Treatments: 7 including untreated check
Application Time : 58 DAS (Days after sowing)
Spray volume : 440 liter/h

Method of Application: Foliar spray with battery operated knap sack sprayer Agronomic Practices : All agronomic practices followed as per the crop requirement except insect control. Observation Methods:
Whitefly (Bemisia tabaci) control: Count the number of live whitefly per leaf. Record the observations from 3 leaves per plant and 10 plants per plot on before spray and 1, 3, 7, 10 and 14 DAA (days after application). Calculate % whitefly control (as observed control) by formula give below:
Number of live whitefly in treated plot
% Whitefly control = 100- x 100
Number of live whitefly in untreated (UTC) plot
Apply colbys formula (given above) to calculate expected control and to judge
synergism.
Fruiting bodies count: Count the number of fruiting bodies (squares, flowers and
bolls) per plant. Record the observations from 10 plants per plot. Table 4: Treatment details

Treatment Number Treatment details Use rate (ml/h) Use rate (g.a.i./h)
Tl Diafenthiuron 16%+Flonicamid 4% OD 1250 200+50
T2 Diafenthiuron 16%+Flonicamid 4% SC 1250 200+50
T3 Diafenthiuron 32%+Flonicamid 8% WG 625 200+50
T4 Diafenthiuron 47.8% SC+Flonicamid 50% WG (tank mix) 418.41 ml+ 100 ml 200+50
T5 Diafenthiuron 47.8% SC 418.41 200
T6 Flonicamid 50% WG 100 g 50
T7 UTC (Untreated Check) 0 0
Table 5a: Control of cotton whitefly

Treatment Number Cotton whitefly, Bemisia tabaci

Observed control (%) at Expected control (%) at

1 DAA 3 DAA 7 DAA 10 DAA 14 DAA 1 DAA 3 DAA 7 DAA 10 DAA 14 DAA
Tl 97.2 94.8 89.6 80.4 67.2 89.51 87.18 82.57 74.20 62.89
T2 95.6 90.2 83 72.8 56.8 89.51 87.18 82.57 74.20 62.89
T3 95.4 89.2 80.6 68.4 51.2 89.51 87.18 82.57 74.20 62.89
T4 93.2 88.6 80.4 68.6 50.4 89.51 87.18 82.57 74.20 62.89
T5 64.8 60.2 54.6 45.8 34.2
T6 70.2 67.8 61.6 52.4 43.6
T7 0.00 0.00 0.00 0.00 0.00

Table 5b: Control of cotton whitefly

Treatment number Colby's ratio Synergism (Yes / No)

1 DAA 3 DAA 7 DAA 10 DAA 14 DAA 1 DAA 3 DAA 7 DAA 10 DAA 14 DAA
Tl 1.09 1.09 1.09 1.08 1.07 Y Y Y Y Y
T2 1.07 1.03 1.01 0.98 0.90 Y Y Y N N
T3 1.07 1.02 0.98 0.92 0.81 Y Y N N N
T4 1.04 1.02 0.97 0.92 0.80 Y Y N N N
T5
T6
T7
The field trial results shows that Tl (Diafenthiuron 16%+Flonicamid 4% OD) provides synergistic control of cotton whitefly up to 14 days after application where as all other conventional treatments (T2, T3 and T4) provides synergistic control upto 3 to 7 days only. The level of control (means percentage) was also observed higher in Tl in comparison with all other conventional treatments (T2, T3 and T4).
Table 6: Fruiting bodies count

Treatment Number Number of fruiting
bodies/plant at 14
DAA % Increase in number of fruiting bodies over
T7 % Increase in number of fruiting bodies in Tl over

T2 T3 T4
Tl 27.6 43.8 7.4 10.8 16.0
T2 25.7 33.9
T3 24.9 29.7
T4 23.8 24.0
T5 20.1 4.7
T6 20.6 7.3
T7 19.2 0.0
Tl (Diafenthiuron 16%+Flonicamid 4% OD) also produces higher number of fruiting bodies as compared to all conventional treatments (T2, T3 and T4).
Overall summery of field trials:
The field trials results shows many benefits/advantages of OD formulation of diafenthiuron+tolfenpyrad and diafenthiuron+flonicamid.
• Synergism observed in terms of insect control
• Provides higher level of insect control (increase in % control)
• Provides residual control (longer duration of control)

• Increases number of fruits, flowers, branches and overall plant growth.
• Overall, OD formulation of Diafenthiuron+Tolfenpyrad and Diafenthiuron+Flonicamid provides better efficacy as compared to conventional formulations.

CLAIMS

We claim;

[CLAIM 1]. An oil-based agrochemical composition comprising:
a. an insecticide Diafenthiuron present in amount of 1.0 to 50%
w/w;
b. an insecticide selected from Tolfenpyrad or Flonicamid present
in amount of 1.0 to 50% w/w;
c. Wetting agent Trisiloxane ethoxylate present in an amount of 2
to 8 % w/w;
d. Carrier solvent Methylated seed oil present in an amount of 40
to 70% w/w;
e. one or more formulation excipients.
[CLAIM 2]. The oil-based agrochemical composition as claimed in claim 1, wherein the formulation for the said composition is Oil dispersion (OD) formulation.
[CLAIM 3]. The oil-based agrochemical composition as claimed in claim 1, wherein the formulation excipients are selected from wetting agent, dispersant or dispersing agent, anti-freezing agent, anti-foam agent, solvent, co-solvents, preservatives, stabilizers, solubilizer and in addition may selected from co-solvent.
[CLAIM 4]. The oil-based agrochemical composition as claimed in claim 1-claim 3, wherein, the Oil Dispersion (OD) formulation comprises:
i. an insecticide Diafenthiuron present in amount of 1.0 to 50%
w/w; ii. an insecticide selected from Tolfenpyrad or Flonicamid present
in amount of 1.0 to 50% w/w; iii. Wetting agent in an amount of 2 to 8 % w/w; iv. Dispersing agent in an amount of 2 to 8 % w/w; v. Emulsifying agent in an amount of 6 to 10 % w/w; vi. Solubilizer in an amount of 0.5 to 4 % w/w;

vii. Stabilizer in an amount of 0.5 to 4% w/w;
viii. Antifoaming agent in an amount of 0.1 to 1.5 % w/w;
ix. Preservative in an amount of 0.1 to 0.5 % w/w;
x. Anti-freezing agent in an amount of 2 to 6 % w/w;
xi. Carrier as solvent in an amount of 40 to 70% w/w.
[CLAIM 5]. The oil-based agrochemical composition as claimed in claim 4, wherein wetting agent is selected from Organosilicone surfactants includes trisiloxane ethoxylate, polydimethylsiloxane, polyoxyethylene methyl polysiloxane, polyoxyalkylene methyl polysiloxane, polyether polymethyl siloxane copolymer, heptamethyl trisiloxane, Polyalkyleneoxide modified heptamethyl trisiloxane, polyether modified polysiloxane, may or may not be in modified form, may be liquid or powder form or mixture thereof.
[CLAIM 6]. The oil-based agrochemical composition as claimed in claim 4, wherein emulsifying agent is selected from castor oil ethoxylates, alcohol ethoxylates, fatty acid ethoxylates, sorbitan ester ethoxylates, sulphosuccinate, calcium salts of dodecylbenzene sulphonate, alkylammonium salts of alkylbenzene sulphonate, alkylsulphosuccinate salts, ethylene oxide-propylene oxide block copolymers, ethoxylated alkylamines, ethoxylated alkyl phenols, polyoxyethylene sorbitan monolaurate.
[CLAIM 7]. The oil-based agrochemical composition as claimed in claim 4, wherein dispersing agent is selected from alkyl sulfonates, alkyl benzene sulfonates, alkyl aryl sulfonates, alkylphenolalkoxylates, tristyrylphenol ethoxylates, natural or synthetic fatty ethoxylate alcohols, natural or synthetic fatty acid alkoxylates, natural or synthetic fatty alcohols alkoxylates, alkoxylated alcohols (such as n-butyl alcohol poly glycol ether), block copolymers (such as ethylene oxide-propylene oxide block copolymers and ethylene oxide-butylene oxide block copolymers), fatty acid-polyalkylene glycol condensates, polyamine-fatty acid condensates, polyester condensates, salts of polyolefin condensates,

sodium ligno sulfonate, sodium ploycarboxylate, EO/PO based copolymer, phenol sulfonate, sodium methyl oleoyl taurate, styrene acrylic acid copolymer, propyleneoxide-ethyleneoxide-copolymer, polyethylene glycol 2,4,6-tristyrylphenyl ether, tristyrylphenol-polyglycol ether-phosphate, tristyrylphenole with 16 moles EO, tristyrylphenol-polyglycolether-phosphate, oleyl-polyglycol ether with ethylene oxide, tallow fattyamine polyethylene oxide, nonylphenol polyglycolether with 9-10 moles ethylene oxide.
[CLAIM 8]. The oil-based agrochemical composition as claimed in claim 4, wherein antifoaming agent is selected from silicone oil, silicone compound, CIO ~C20 saturated fat acid compounds or C8~C10 aliphatic alcohols compound, silicone antifoam emulsion, dimethylsiloxane, polydimethyl siloxane, vegetable oil based antifoam, tallow based fatty acids, polyalkyleneoxide modified polydimethyl siloxane.
[CLAIM 9]. The oil-based agrochemical composition as claimed in claim 4, wherein Carrier as solvent is selected from alkylated vegetable oil may be methylated vegetable oil or ethylated vegetable oil. The vegetable oils include olive oil, kapok oil, castor oil, papaya oil, camellia oil, sesame oil, corn oil, rice bran oil, cotton seed oil, soybean oil, groundnut oil, rapeseed-mustard oil, linseed oil, tung oil, sunflower oil, safflower oil, coconut oil. The alkyl ester of vegetable oils includes methyl ester, ethyl ester, propyl ester or butyl ester of vegetable oils. Some of the examples are methylated seed oil, polyalkyleneoxide modified polydimethylsiloxane alkylphenol ethoxylate, rapeseed oil methyl ester, rapeseed oil ethyl ester, rapeseed oil propyl esters, rapeseed oil butyl esters, soybean oil methyl ester, soybean oil ethyl ester, soybean oil propyl ester, soybean oil butyl ester, castor oil methyl ester, castor oil ethyl ester, castor oil propyl ester, castor oil butyl ester, cotton seed oil methyl ester, cotton seed oil ethyl ester, cotton seed oil butyl ester, cotton seed oil propyl ester, tall oil fatty acids esters-tallow methyl ester, tallow ethyl ester, tallow propyl ester, bio-diesel, mineral oil (aromatic solvents, isoparaffin, base solvent), fatty acid amides (e.g. CI -C3 amines, alkylamines or alkanolamines with C6-C18 carboxylic acids), fatty acids, alkyl esters of fatty acids, methyl and ethyl oleate, methyl and ethyl soyate, alkyl benzenes and alkylnaphthalenes, polyalkylene glycol ethers, fatty acid diesters, fatty alkylamides and diamides, dialkylene carbonates, ketones and alcohols; or misture thereof.
[CLAIM 10]. The oil-based agrochemical composition as claimed in claim 4, wherein preservative is selected from l,2-benzisothiazolin-3(2H)-one, sodium salt, sodium benzoate, 2-bromo-2-nitropropane-l,3-diol, formaldehyde, sodium o-phenylphenate, 5-chloro-2-methyl-4-isothiazolin-3-one & 2-methyl-4-isothiazolin-3-one.
[CLAIM 11]. The oil-based agrochemical composition as claimed in claim 4, wherein solubilizer is Polyvinyl pyrrolidone.
[CLAIM 12]. The oil-based agrochemical composition as claimed in claim 4, wherein stabilizer is selected from hectorite clay, aluminum magnesium silicate, bentonite clay, silica, attapulgite clay.
[CLAIM 13]. The oil-based agrochemical composition as claimed in claim 4, wherein anti-freezing agent is selected from ethylene glycol, propane diols, glycerine or the urea, glycol (Monoethylene glycol, Diethylene glycol, Polypropylene glycol, Polyethylene glycol), glycerine, urea, magnesium sulfate heptahydrate, sodium chloride.
[CLAIM 14]. The oil-based agrochemical composition as claimed in claim 3, wherein formulation excipient in addition comprises co-solvent selected from Cyclohexanone, Acetophenone, NMP, Dimethyl sulfoxide, Benzyl alcohol, Butanol, N-octanol, N-Propanol, 2-ethyl hexanol, Tetrahydro furfuryl alcohol, Isophorone, Fatty acid dimethyl amide, 2-hexylethyl lactate, Propylene carbonate.