The scope of the claims

[Requested item 1]

　Following one of the S1 group, second S7 group seven primer pairs obtained by selecting one primer pair for each group, for bacterial DNA amplification PCR, characterized in that it comprises at least one primer pair primer set.
　The S1
group: 1-1A: a forward primer consisting of the sequences SEQ ID NO: 1, comprising a combination of a reverse primer consisting of any one of the sequences of SEQ ID NO: 22 and 27 primer
pairs, 1-2A: sequence of SEQ ID NO: 10 a forward primer consisting of a combination of a reverse primer consisting of any one of the sequences of SEQ ID NO: 20 and 21 primer pairs,
1-3A: a forward primer consisting of the sequences SEQ ID NO: 11, SEQ ID NO: 16, 18 and 26 any one consisting sequence primers consisting of a combination of the reverse primer pairs,
1-4: a forward primer consisting of the sequences SEQ ID NO: 12, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 16-27 consisting of primer
pairs, 1- : A forward primer consisting of the sequences SEQ ID NO: 13, SEQ ID NO: 16-27 any one of a combination of a reverse primer consisting of the sequence primer pairs,
1-7: a forward primer consisting of the sequences SEQ ID NO: 15, SEQ primer pair comprising a combination of a reverse primer consisting of any one of the sequences of numbers 16 -
27, 1-8a: a forward primer consisting of the sequences SEQ ID NO: 6, SEQ ID NO: 16, 19 ~ 21,23,24,26 and 27 one of the primer pair comprising a combination of a reverse primer consisting of the
sequences, 1-9a: a forward primer consisting of the sequences SEQ ID NO: 7, or SEQ ID NO: 16 to 21,23,24,26, and 27 1 a combination of a reverse primer consisting One sequence Consisting primer pair or,
1-10a: a forward primer consisting of the sequences SEQ ID NO: 8, SEQ ID NO: 16 to 21,23,24,26, and 27 one of the primer pair comprising a combination of a reverse primer consisting of the sequences,
　the S2
group: 2-1A: a forward primer consisting of the sequences SEQ ID NO: 28, SEQ ID NO: 45 and 47-50 one of a combination of a reverse primer consisting of the sequence primer
pairs, 2-2: SEQ ID NO: 29 a forward primer consisting of the sequences, SEQ ID NO: 44,45,47,48 and 50 one of the primer pair comprising a combination of a reverse primer consisting of the
sequences, 2-3: a forward primer consisting of the sequences SEQ ID NO: 30 , any of SEQ ID NO: 45 and 47-50 1 Sequence primers comprising a combination of the reverse primer pair consisting of
2-4: a forward primer consisting of the sequences SEQ ID NO: 31, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 46 and 47 primer pairs,
2-5: a forward primer consisting of the sequences SEQ ID NO: 32, SEQ ID NO: 45 and 47-49 one of a combination of a reverse primer consisting of the sequence primer
pairs, 2-6a: consisting of the sequences SEQ ID NO: 33 and forward primer, SEQ ID NO: 44, 45 and 47-50 one of the primer pair comprising a combination of a reverse primer consisting of the
sequences, 2-7A: a forward primer consisting of the sequences SEQ ID NO: 34, SEQ ID NO: 45 and 47 and any 48 Primer pair comprising a combination of a reverse primer consisting of one of the sequences,
2-8A: a forward primer consisting of the sequences SEQ ID NO: 35, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 44,45,48 and 49 primer pair consisting
of, 2-9A: a forward primer consisting of the sequences SEQ ID NO: 36, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 44,45,48 and 49 primer
pairs, 2-10A: a forward primer consisting of the sequences SEQ ID NO: 37, SEQ ID NO: 45 and 47-50 one of a combination of a reverse primer consisting of the sequence primer pairs,
2-11: a forward primer consisting of the sequences SEQ ID NO: 38, SEQ ID NO: 44 Primer pair comprising a combination of a reverse primer consisting of any one of the sequences of 50,
2-12A: a forward primer consisting of the sequences SEQ ID NO: 39, a combination of any one of the reverse primer of SEQ ID NO: 44-48 and 50 becomes primer
pair 2-13: a forward primer consisting of the sequences SEQ ID NO: 40, SEQ ID NO: 44-50 any one of a combination of a reverse primer consisting of the sequence primer
pairs, 2-14A: sequence of SEQ ID NO: 41 a forward primer consisting of SEQ ID NO: 45 and 46 one of the primer pair comprising a combination of a reverse primer consisting of the sequences,
2-15A: a forward primer consisting of the sequences SEQ ID NO: 42, any of SEQ ID NO: 48 and 49 one Primer pair consisting of a combination of the reverse primer consisting of the sequences or,
2-16A: a forward primer consisting of the sequences SEQ ID NO: 43, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 44, 46 to 48 and 50 primer pair consisting of
　the S3 group:
　a forward primer consisting of the sequences SEQ ID NO: 51, a combination of reverse primer consisting of the sequences SEQ ID NO: 52 primer pair
　No. S4 group:
4-1: consisting of the sequences SEQ ID NO: 53 and forward primer, primer pair consisting of a combination of the reverse primer consisting of any one of the sequences of SEQ ID NO: 57-61,
4-2: a forward primer consisting of the sequences SEQ ID NO: 54, or SEQ ID NO: 59 and 61 1 Li consisting One of the array Primer pair consisting of a combination of Bath primers
4-3: a forward primer consisting of the sequences SEQ ID NO: 55, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 57-59 and No. 61 primer pairs or,
4-4: a forward primer consisting of the sequences SEQ ID NO: 56, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 57-59 and 61 primer pairs
　the S5 group
5-1A: sequence of SEQ ID NO: 62 a forward primer consisting of a combination of a reverse primer consisting of any one of the sequences of SEQ ID NO: 70, 73 to 75 primer
pairs, 5-2: a forward primer consisting of the sequences SEQ ID NO: 63, SEQ ID NO: 70 to 75 from any one of the sequences of Primer pair consisting of a combination of the reverse primer that,
5-3: a forward primer consisting of the sequences SEQ ID NO: 64, a primer pair consisting of a combination of the reverse primer consisting of any one of the sequences of SEQ ID NO: 70-75,
5-4 : a forward primer consisting of the sequences SEQ ID NO: 65, SEQ ID NO: 70-75 any one of a combination of a reverse primer consisting of the sequence primer pairs,
5-5a: a forward primer consisting of the sequences SEQ ID NO: 66, SEQ primer pair comprising a combination of a reverse primer consisting of any one of SEQ numbers 70,72,73 and
75, 5-6A: a forward primer consisting of the sequences SEQ ID NO: 67, SEQ ID NO: 70, 71 and 73-75 from any one of the array Primer consisting of a combination of the reverse primer pairs that,
5-7: a forward primer consisting of the sequences SEQ ID NO: 68, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 70-75 primer pair or,
5- 8: a forward primer consisting of the sequences SEQ ID NO: 69, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 70-75 primer pair
　No. S6 group
6-1A: forward primer consisting of the sequences SEQ ID NO: 76 When the primer pair consisting of a combination of the reverse primer consisting of any one of the sequences of SEQ ID NO: 93, 96 and
101-103, 6-2: a forward primer consisting of the sequences SEQ ID NO: 77, SEQ ID NO: 91, 96 and 101 one of the ~ 103 Primer pair comprising a combination of a reverse primer consisting of one of the sequences,
6-3: a forward primer consisting of the sequences SEQ ID NO: 78, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 91 and 101-103 primer
pair, 6-4a: a forward primer consisting of the sequences SEQ ID NO: 79, SEQ ID NO: 91 and 101-103 in any one of the primer pair comprising a combination of a reverse primer consisting of the
sequences, 6-5: SEQ ID NO: 80 a forward primer consisting of SEQ, SEQ ID NO: 91, 93 and primer pair consisting of a combination of the reverse primer consisting of 100-103 sequence of any one of,
6-6: a forward primer consisting of the sequences SEQ ID NO: 81, SEQ ID NO: 91, 3 and 101 - 103 or one primer pair comprising a combination of a reverse primer consisting of the
sequences, 6-7: a forward primer consisting of the sequences SEQ ID NO: 82, any of SEQ ID NO: 91,96,97 and 101-103 one of a combination of a reverse primer consisting of the sequence primer pair
6-8: a forward primer consisting of the sequences SEQ ID NO: 83, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 91 and 101 to 103 becomes primer
pair 6-9: a forward primer consisting of the sequences SEQ ID NO: 84, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 91 and 101-103 primer
pair 6-10: SEQ ID NO: 85 follower consisting of the sequence Over de primer, SEQ ID NO: 91 and 101-103 in any one of the primer pair comprising a combination of a reverse primer consisting of the
sequences, 6-11A: a forward primer consisting of the sequences SEQ ID NO: 86, SEQ ID NO: 91, 93 and 101 to 103 one of the primer pair comprising a combination of a reverse primer consisting of the
sequences, 6-12: a forward primer consisting of the sequences SEQ ID NO: 87, composed of any one sequence of SEQ ID NO: 91, 96 and 101-103 primer pair consisting of a combination of the reverse primer,
6-13: a forward primer consisting of the sequences SEQ ID NO: 88, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 91 and 101-103 primer pair
6 14: a forward primer consisting of the sequences SEQ ID NO: 89, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 91 and 101-103 primer pair or
6-15: Forward consisting of the sequences SEQ ID NO: 90 primer, SEQ ID NO: 91,92,96 and 101 to any one each consisting of a combination of a reverse primer consisting of the sequence primer pairs 103
　first S7 group
7-1A: a forward primer consisting of the sequences SEQ ID NO: 104, SEQ ID NO: 5 and 118 to 125,127,128,130 and 131 one of the primer pair comprising a combination of a reverse primer consisting of the
sequences, 7-2A: a forward primer consisting of the sequences SEQ ID NO: 105, SEQ ID NO: 5 and 118 to Primer pair comprising a combination of a reverse primer consisting of any one of the sequences of
25, 7-3a: a forward primer consisting of the sequences SEQ ID NO: 106, any one of the sequences of SEQ ID NO: 2, 5, and 116 and 118 to 131 primer pair comprising a combination of a reverse primer consisting of,
7-4a: a forward primer consisting of the sequences SEQ ID NO: 107, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 120 and 121 primer pairs,
7- 5A: a forward primer consisting of the sequences SEQ ID NO: 108, SEQ ID NO: 120 and 123 one of a combination of a reverse primer consisting of the sequence primer
pairs, 7-6a: forward ply consisting of the sequences SEQ ID NO: 109 Chromatography and, SEQ ID NO: 2,118 ~ 122, 124 and 126 one of the primer pair comprising a combination of a reverse primer consisting of the
sequences, 7-7A: a forward primer consisting of the sequences SEQ ID NO: 110, SEQ ID NO: 118, 120,121,124 and 126 to primer pairs consisting of a combination of a reverse primer consisting of any one sequence of
130, 7-8A: a forward primer consisting of the sequences SEQ ID NO: 111, SEQ ID NO: 2, 5, and 118 to 131 any one primer pair comprising a combination of a reverse primer consisting of the
sequences, 7-9A: a forward primer consisting of the sequences SEQ ID NO: 112, SEQ ID NO: 2, 5, and 116, and 118 to any one of the sequences of 125 reverse primer consisting of Primer pair consisting viewed
alignment, 7-10A: a forward primer consisting of the sequences SEQ ID NO: 113, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 2,5,118-126, 130 and 131 primers
pair, 7-11A: a forward primer consisting of the sequences SEQ ID NO: 114, SEQ ID NO: 5 primer pair consists of a combination of the reverse primer consisting of the sequences or,
7-12A: a forward primer consisting of the sequences SEQ ID NO: 115, SEQ primer pair comprising a combination of a reverse primer consisting of any one of the sequences of numbers 2 and 5.

[Requested item 2]

　Following 1) the seven primer pairs to 7), the primer set according to claim 1 comprising at least one primer pair.
1) a forward primer consisting of the sequences SEQ ID NO: 15, combined primer pair consisting of a reverse primer comprising a sequence of SEQ ID NO: 16,
2) a forward primer consisting of the sequences SEQ ID NO: 43, reverse consisting of the sequences SEQ ID NO: 50 primer pair consisting of primers,
3) a forward primer consisting of the sequences SEQ ID NO: 51, combined primer pair consisting of a reverse primer comprising a sequence of SEQ ID NO: 52,
4) a forward primer consisting of the sequences SEQ ID NO: 53, combinations primer pair consisting of a reverse primer comprising a sequence of SEQ ID NO: 57,
5) and a forward primer consisting of the sequences SEQ ID NO: 62, a combination of reverse primer consisting of the sequences SEQ ID NO: 70 primer Pair,
6) and a forward primer consisting of the sequences SEQ ID NO: 81, combined primer pair consists of the reverse primer comprising a sequence of SEQ ID NO: 103, and
7) and a forward primer consisting of the sequences SEQ ID NO: 115, the sequence of SEQ ID NO: 5 primer pair consisting of a combination of the reverse primer consisting of.

[Requested item 3]

　Wherein 1) the primer set according to claim 2, including any one of the primer pairs 1-7).

[Requested item 4]

　Primer set according to claim 3 comprising any one of the following combinations of A ~ G.
In case of containing a set of primers (A) wherein 1) further comprises at least three primer pairs selected from the 2) to 7).
(B) In the case of containing a primer set of the two), further comprising said 1) and 3) at least three primer pairs are selected from 1-7).
When containing primer pairs (C) the 3), 1), 2) and 4) further comprises at least three primer pairs are selected from 1-7).
When containing a primer pair of the (D) above 4), further comprising the 1) to 3) and 5) at least three primer pairs are selected from 1-7).
When containing primer pairs (E) the 5), further including the 1) to 4) and 6) at least three primer pairs are selected from 1-7).
(F) if it contains a primer pair of the 6), further including the 1) to 5) at least three primer pairs are selected from and 7).
When containing primer pairs (G) above 7), further comprising at least three primer pairs selected from the 1) to 6).

[Requested item 5]

　Primer set according to any one of claims 1 to 4, which is used for bacterial species detected.

[Requested item 6]

　Primer set according to claim 4 which is used for bacterial species detected and / or identified.

[Requested item 7]

　The seven of the primer pair, the primer set for bacterial species identification according to any one of claims 1 to 5, comprising at least four primer pairs.

[Requested item 8]

　Following one of the K1 group, second K7 group seven primer pairs obtained by selecting one primer pair for each group, the bacterial species detection kit, characterized in that it comprises at least one primer pair.
　The K1
Group: 1-1: a forward primer consisting of the sequences SEQ ID NO: 1, SEQ ID NO: 16-27 any one of a combination of a reverse primer consisting of the sequence primer
pairs, 1-2: sequence of SEQ ID NO: 10 a forward primer consisting of a primer pair consisting of a combination of the reverse primer consisting of any one of the sequences of SEQ ID NO: 16, 18, 20 and
21, 1-3: a forward primer consisting of the sequences SEQ ID NO: 11, SEQ ID NO: 16 to 21 and 23-26 one of the primer pair comprising a combination of a reverse primer consisting of the sequences,
1-4: a forward primer consisting of the sequences SEQ ID NO: 12, from any one of SEQ ID NO: 16-27 primer pair consisting of a combination of the reverse primer comprising ,
1-5: a forward primer consisting of the sequences SEQ ID NO: 13, SEQ ID NO: 16-27 any one of a combination of a reverse primer consisting of the sequence primer pairs,
1-6: Forward consisting of the sequences SEQ ID NO: 14 primer, a primer pair consisting of a combination of the reverse primer consisting of any one of the sequences of SEQ ID NO: 16-27,
1-7: a forward primer consisting of the sequences SEQ ID NO: 15, any one of SEQ ID NO: 16 to 27 primer pair comprising a combination of a reverse primer consisting of the sequence,
1-8: a forward primer consisting of the sequences SEQ ID NO: 6, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 16-27 primer pair
1 -9: I from the sequence of SEQ ID NO: 7 And forward primer, primer pair consisting of a combination of the reverse primer consisting of any one of the sequences of SEQ ID NO: 16-27,
1-10: a forward primer consisting of the sequences SEQ ID NO: 8, or SEQ ID NO: 16-27 1 one primer pair consists of a combination of the reverse primer consisting of the sequence or,
1-11: a forward primer consisting of the sequences SEQ ID NO: 9, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 16, 17, 19 and 26 primer pair consisting of
　the K2
group: 2-1: primer consisting of a reverse primer consisting of any one sequence of a forward primer consisting of the sequences SEQ ID NO: 28, SEQ ID NO: 44, 45 and SEQ ID NO: 47-50
pair, 2-2: SEQ ID NO A forward primer consisting of the sequence of issue 29, SEQ ID NO: 44,45,47,48 and 50 one of the primer pair comprising a combination of a reverse primer consisting of the
sequences, 2-3: Forward consisting of the sequences SEQ ID NO: 30 a primer, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 45 and 47-50 primer pair
2-4: a forward primer consisting of the sequences SEQ ID NO: 31, or SEQ ID NO: 46 and 47 primer pair comprising a combination of a reverse primer consisting of one of the sequences,
2-5: a forward primer consisting of the sequences SEQ ID NO: 32, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 45 and 47-49 primer
pair, 2-6: A forward primer consisting of the sequences SEQ ID NO: 33, SEQ ID NO: 44-50 or one primer pair comprising a combination of a reverse primer consisting of the sequences,
2-7: a forward primer consisting of the sequences SEQ ID NO: 34, SEQ ID NO: primer pair comprising a combination of a reverse primer consisting of 45 to one of sequences 50,
2-8: a forward primer consisting of the sequences SEQ ID NO: 35, composed of any one sequence of SEQ ID NO: 44-50 reverse primer primer pair consisting of a combination of
2-9: a forward primer consisting of the sequences SEQ ID NO: 36, a primer pair consisting of a combination of the reverse primer consisting of any one of the sequences of SEQ ID NO: 44-46 and 48-50,
2- 10: coordination of SEQ ID NO: 37 A forward primer consisting of columns, SEQ ID NO: 45-50 or one primer pair comprising a combination of a reverse primer consisting of the sequences,
2-11: a forward primer consisting of the sequences SEQ ID NO: 38, SEQ ID NO: 44-50 primer pair comprising a combination of a reverse primer consisting of any one sequence,
2-12: a forward primer consisting of the sequences SEQ ID NO: 39, a primer pair consisting of a combination of any one of the reverse primer of SEQ ID NO: 44-50,
2-13: a forward primer consisting of the sequences SEQ ID NO: 40, SEQ ID NO: 44-50 any one of a combination of a reverse primer consisting of the sequence primer pairs,
2-14: forward primer consisting of the sequences SEQ ID NO: 41 and, distribution Column numbers 44-50 any one primer pair comprising a combination of a reverse primer consisting of the sequences,
2-15: a forward primer consisting of the sequences SEQ ID NO: 42, composed of any one sequence of SEQ ID NO: 44-50 primer pair or a combination of the reverse primer
2-16: a forward primer consisting of the sequences SEQ ID NO: 43, SEQ ID NO: 44-48 and primer pair consisting of a combination of the reverse primer consisting of any one of the sequences of 50,
　the K3 groups:
　a forward primer consisting of the sequences SEQ ID NO: 51, SEQ ID NO: 52 SEQ combination primer pair consisting of a reverse primer consisting of
　the K4 group:
4-1: a forward primer consisting of the sequences SEQ ID NO: 53, SEQ ID NO: one of the 57-61 One consists of the sequence primer consisting of the combination of the reverse primer pairs
4-2: a forward primer consisting of the sequences SEQ ID NO: 54, a primer pair consisting of a combination of the reverse primer consisting of any one of the sequences of SEQ ID NO: 59 and 61 ,
4-3: a forward primer consisting of the sequences SEQ ID NO: 55, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 57-59 and 61 primer pairs or
4-4: sequence of SEQ ID NO: 56 a forward primer consisting of SEQ ID NO: 57-59 and any one of a combination of a reverse primer consisting of the sequence primer pairs 61
　the K5 group
5-1: the forward primer consisting of the sequences SEQ ID NO: 62, SEQ ID NO: 70 , 71 and 73-7 Any one consisting sequence primers consisting of a combination of the reverse primer pairs,
5-2: a forward primer consisting of the sequences SEQ ID NO: 63, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 70-75 becomes primer pair
5-3: a forward primer consisting of the sequences SEQ ID NO: 64, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 70-75 primer pair
5-4: sequence of SEQ ID NO: 65 a forward primer consisting of SEQ ID NO: 70-75 or one primer pair comprising a combination of a reverse primer consisting of the sequences,
5-5: a forward primer consisting of the sequences SEQ ID NO: 66, any of SEQ ID NO: 70-75 or Li consisting of one of the array Over scan primer primer pair consisting of
5-6: a forward primer consisting of the sequences SEQ ID NO: 67, SEQ ID NO: 70-75 or one primer pair comprising a combination of a reverse primer consisting of the sequences,
5-7: sequence a forward primer consisting of the sequences ID NO: 68, SEQ ID NO: 70-75 any one sequence comprising a combination of a reverse primer comprising a primer pair or
5-8: a forward primer consisting of the sequences SEQ ID NO: 69, SEQ ID NO: 70 to consist of a combination of a reverse primer consisting of any one of the sequences of 75 primer pair
　No. K6 group
6-1: the forward primer consisting of the sequences SEQ ID NO: 76, any of SEQ ID NO: 91,93,96 and 101-103 or consisting of one of the array Primer pair consisting of a combination of the reverse primer,
6-2: a forward primer consisting of the sequences SEQ ID NO: 77, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 91, 96 and 101-103 primer pair,
6-3: a forward primer consisting of the sequences SEQ ID NO: 78, SEQ ID NO: 91 and 101-103 in any one of a combination of a reverse primer consisting of the sequence primer
pairs, 6-4: consisting of the sequences SEQ ID NO: 79 and forward primer, SEQ ID NO: 91 and 97 and 101-103 in any one of the primer pair comprising a combination of a reverse primer consisting of the
sequences, 6-5: a forward primer consisting of the sequences SEQ ID NO: 80, SEQ ID NO: 91 and 93 and 100 to 103 one of the primer pair comprising a combination of a reverse primer consisting of the
sequences, 6-6: a forward primer consisting of the sequences SEQ ID NO: 81, composed of any one sequence of SEQ ID NO: 91, 93 and 101-103 primer pair consisting of a combination of the reverse
primer, 6-7: a forward primer consisting of the sequences SEQ ID NO: 82, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 91,96,97 and 101-103 primers
pair, 6-8: a forward primer consisting of the sequences SEQ ID NO: 83, SEQ ID NO: 91 and 101 to 103 of any one of a combination of a reverse primer consisting of the sequence primer
pairs, 6-9: sequence of SEQ ID NO: 84 forward plies consisting of And mer, SEQ ID NO: 91 and 101-103 in any one of the primer pair comprising a combination of a reverse primer consisting of the
sequences, 6-10: a forward primer consisting of the sequences SEQ ID NO: 85, SEQ ID NO: 91 and 101-103 primer pair comprising a combination of a reverse primer consisting of any one
sequence, 6-11: a forward primer consisting of the sequences SEQ ID NO: 86, composed of any one sequence of SEQ ID NO: 91,93,97 and 101-103 primer pair consisting of a combination of the reverse
primer, 6-12: a forward primer consisting of the sequences SEQ ID NO: 87, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 91, 96 and 101-103 primer pair,
6-13: A forward primer consisting of the sequences SEQ ID NO: 88, SEQ ID NO: 91 and 101-103 in any one of a combination of a reverse primer consisting of the sequence primer pairs,
6-14: a forward primer consisting of the sequences SEQ ID NO: 89, SEQ ID NO: 91 and 101-103 in any one of the primer pairs consisting of a combination of the reverse primer consisting of the sequences or
6-15: a forward primer consisting of the sequences SEQ ID NO: 90, SEQ ID NO: 91,92,96 and 101 to primer pairs each consisting of a combination of a reverse primer consisting of any one sequence of 103
　second K7 group
7-1: the forward primer consisting of the sequences SEQ ID NO: 104, or SEQ ID NO: 2, 5, and 116 to 131 1 River consisting One of the array Primer pair consisting of the scan primers
7-2: a forward primer consisting of the sequences SEQ ID NO: 105, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 2, 5, and 116 to 131 primer pairs,
7-3: a forward primer consisting of the sequences SEQ ID NO: 106, SEQ ID NO: 2, 5, and 116 to 131 of any one of a combination of a reverse primer consisting of the sequence primer
pairs, 7-4: sequence of SEQ ID NO: 107 forward primer and a primer pair consisting of a combination of the reverse primer consisting of any one of the sequences of SEQ ID NO: 2, 5, and 116 to 131 consisting of,
7-5: a forward primer consisting of the sequences SEQ ID NO: 108, SEQ ID NO: 2 , of 5 and 116-131 Primer pair comprising a combination of a reverse primer consisting of any one sequence,
7-6: a forward primer consisting of the sequences SEQ ID NO: 109, consisting of any one of the sequences of SEQ ID NO: 2, 5, and 116 to 131 reverse primer primer pair consisting of a combination
of 7-7: a forward primer consisting of the sequences SEQ ID NO: 110, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO: 2, 5, and 116 to 131 primer
pairs, 7- 8: a forward primer consisting of the sequences SEQ ID NO: 111, SEQ ID NO: 2, 5, and 116 to 131 of any one of a combination of a reverse primer consisting of the sequence primer
pairs, 7-9: consisting of the sequences SEQ ID NO: 112 and forward primer, distribution Primer pair comprising a combination of a reverse primer consisting of any one sequence of column numbers 2, 5, and
116-131, 7-10: a forward primer consisting of the sequences SEQ ID NO: 113, SEQ ID NO: 2, 5, and 116 to 131 of one of the primer pair comprising a combination of a reverse primer consisting of the sequence,
7-11: a forward primer consisting of the sequences SEQ ID NO: 114, consisting of any one of the sequences of SEQ ID NO: 2, 5 and 125 of the reverse primer comprising a combination primer pair or
7-12: a forward primer consisting of the sequences SEQ ID NO: 115, a combination of reverse primer consisting of any one of the sequences of SEQ ID NO 2,5,116,118,123 and 125-128 consisting of primer pairs.

[Requested item 9]

　Following 1) of the seven primers pairs 1-7) The kit according to claim 8 comprising at least one primer pair.
1) a forward primer consisting of the sequences SEQ ID NO: 15, combined primer pair consisting of a reverse primer comprising a sequence of SEQ ID NO: 16,
2) a forward primer consisting of the sequences SEQ ID NO: 43, reverse consisting of the sequences SEQ ID NO: 50 primer pair consisting of primers,
3) a forward primer consisting of the sequences SEQ ID NO: 51, combined primer pair consisting of a reverse primer comprising a sequence of SEQ ID NO: 52,
4) a forward primer consisting of the sequences SEQ ID NO: 53, combinations primer pair consisting of a reverse primer comprising a sequence of SEQ ID NO: 57,
5) and a forward primer consisting of the sequences SEQ ID NO: 62, a combination of reverse primer consisting of the sequences SEQ ID NO: 70 primer Pair,
6) and a forward primer consisting of the sequences SEQ ID NO: 81, combined primer pair consists of the reverse primer comprising a sequence of SEQ ID NO: 103, and
7) and a forward primer consisting of the sequences SEQ ID NO: 115, the sequence of SEQ ID NO: 5 primer pair consisting of a combination of the reverse primer consisting of.

[Requested item 10]

　Wherein 1) to 7) The kit according to claim 9 comprising any one of the primer pairs.

[Requested item 11]

　The kit of claim 10 comprising any one of the following combinations of A ~ G.
In case of containing a set of primers (A) wherein 1) further comprises at least three primer pairs selected from the 2) to 7).
(B) In the case of containing a primer set of the two), further comprising said 1) and 3) at least three primer pairs are selected from 1-7).
When containing primer pairs (C) the 3), 1), 2) and 4) further comprises at least three primer pairs are selected from 1-7).
When containing a primer pair of the (D) above 4), further comprising the 1) to 3) and 5) at least three primer pairs are selected from 1-7).
When containing primer pairs (E) the 5), further including the 1) to 4) and 6) at least three primer pairs are selected from 1-7).
(F) if it contains a primer pair of the 6), further including the 1) to 5) at least three primer pairs are selected from and 7).
When containing primer pairs (G) above 7), further comprising at least three primer pairs selected from the 1) to 6).

[Requested item 12]

　The kit of claim 11 which is used for bacterial species detected and / or identified.

[Requested item 13]

　The seven of the primer pairs, kits for bacterial species identification according to any one of claims 8-11 comprising at least 4 primer pairs.

[Requested item 14]

　A forward primer consisting of the sequences SEQ ID NO: 1, any one of claims 8 to 13, further comprising an additional primer pair and at least one selected from the reverse primer consisting of any one sequence of SEQ ID NO: 2-5 the kit according to (1).

[Requested item 15]

　It said additional and forward primer primer pair consisting of the sequences SEQ ID NO: 1, claim 14 The kit of wherein the primer pair of reverse primer consisting of the sequences SEQ ID NO: 5.

[Requested item 16]

　The kit according to any one of claims 8 to 15, further comprising a PCR enzyme.

[Requested item 17]

　Primers and the PCR enzymes individually or whole kit according to claim 16, the nucleic acid contamination of bacterial origin is less than 10 fg.

[Requested item 18]

　pH buffer, dNTPs, MgCl 2 kit according to claim 16, further comprising at least one of and measuring container.

[Requested item 19]

　Primers, PCR enzymes, pH buffering solution, dNTPs, MgCl 2 as individual or total and measuring container, and wherein the nucleic acid contamination of bacterial origin is less than 10 fg, kit of claim 18.

[Requested item 20]

　Primers, PCR enzymes, pH buffering solution, dNTPs, MgCl 2 and measuring container is packaged individually, kit of claim 19 nucleic mixed amount from bacteria in each packaging is less than 10 fg.

[Requested item 21]

　The kit of claim 16 or 17 further comprising a fluorescent dye.

[Requested item 22]

　Primers, PCR enzymes, pH buffering solution, dNTPs, fluorescent dyes, MgCl 2 as individual or total and measuring container, and wherein the bacterial contamination of bacterial origin is less than 10 fg, according to claim 21, wherein the bacteria species identification kit.

[Requested item 23]

　Primers, PCR enzymes, pH buffering solution, dNTPs, fluorescent dyes, MgCl 2 and measuring container is packaged individually, according to claim 22 nucleic mixed amount from bacteria within each package is less than 10fg kit.

[Requested item 24]

　Gamma ray treatment kit of claim 19, 20, 22 or 23 nucleic acid contamination of bacterial origin is less than or equal to 10fg by any one of the processes of ultrafiltration and EMA treatment is performed.

[Requested item 25]

　dNTP is chemically modified dNTP for hot start PCR, kit according to any one of claims 18-20 and 22-23.

[Requested item 26]

　PCR enzyme is a Taq DNA Polymerase under DNA content 10 fg / [mu] L of bacterial origin, kit according to any one of claims 16-25.

[Requested item 27]

　In the detection target bacterial species detection and / or identification method in a specimen,
　and the bacterial genomic DNA prepared from the sample, a primer for obtaining an amplification product containing the target gene specific to the detection target bacterial species, heat resistance and performing PCR using a DNA polymerase,
　a step of performing detection of the target gene in the amplification product in the PCR, or by analysis of the amplification products, the detection or identification of detected bacterial species during the sample If,
have,
　using primer set according to any one of claims 1 to 19 as the primers
bacterial species identification method, characterized in that.

[Requested item 28]

　Bacterial species identification method of claim 27 using the kit as described in any one of claims 8-26.