Field of the invention
The present invention relates to personal care compositions. More particularly the
invention relates to a personal care composition comprising Tecomella undulata extract.
Background and prior art of the invention
Tecomella undulata is a deciduous or nearly evergreen tree of arid and semi arid
regions. It occurs on flat and undulating areas including gentle hill slopes and
sometimes also in ravines.Tecomella undulata is mainly used as a source of timber. Its
wood is strong, tough and durable. It takes a fine finish. Heartwood and roots of the
plant contains quinoid. A perusual of literature reveals that quinonoid constituents like
radermachol, 2-isopropenylnaphtho[2,3-b]furan-4,9-quinone, a-lapachone,dehydro-a-
lapachone,P-lapachone,stigmasterol and P-sitosterol have been reported in the
heartwood of the plant.
Pharmacological and phytochemical review on Tecomella undulata- Nagpal N,
Arora M, Rahar S, Swami G and Kapoor R, teaches Tecomella undulata is a
monotypic genus and one of the most important deciduous, ornamental shrub or small
tree of the arid zone regions in India. Different chemical constituents such as
Radermachol, Undulatin, Lapachol, Tecomelloside, Stigmasterol, P-amyrin, p-sitosterol,
p-sitosteryl acetate, compesterol, stigmasterol and many others have been isolated from
the plant. Heartwood and bark of young branches is employed for the treatment of
various diseases of skin, liver, spleen, urinary tract and central nervous system. Leaves
shows significant antimicrobial activity and are used in treatment of AIDS. It is a
common agroforestry tree species in the Thar Desert of Rajasthan known for its higher
survival rates even in extreme drought conditions. The present article reviews the
phytochemical and pharmacological work done on the plant.
Mahendra Jain et.al., traditional uses, phytochemistry and pharmacology of
Tecomella undulata- A review; Asian Pacific Journal of Tropical Biomedicine

(2012) S1918-S1923j_relates to photochemistry and pharmacology of Tecomella
undulata (Family, Bignoniacea), and discusses its future scope of research. Authors of
this review disclose that phytochemical studies of plant parts have led to identification
of many pharmacologically relevant compounds such as Iridoid Glucoside,
naphthoquinone, phytosterols, flavonoid glycoside, flavonol, and triterpenoids. The
review also discusses the plant extract reputation and considerable medicinal value in
Ayurveda and traditional medicine as it is extensively used for treatment of several
diseases like liver, spleen, internal tumors, and diseases of abdomen, wound healing,
conjunctivitis, hepato splenomegaly, syphilis, gonorrhea and hepatitis.
In a TKDL citation AT/119 Tecomella undulata has been used along with other herbal
extracts only for the treatment of enlarged liver, abdominal lump, diseases of the
abdomen etc. So the property of Tecomella undulata in curing age related skin problems
and other skin related diseases was not known traditionally.
Highly pleasing skin appearance is one of the most desired expectations from personal
care products from most consumers around the world. In tropical countries, skin is
continuously subjected to deterioration through dermatological disorders, environmental
abuse (wind, air conditioning, central heating) or through the normal ageing process
(chrono-ageing) which may be accelerated by exposure of skin to sun (photo-ageing).
In recent years, the demand for cosmetic methods for improving the appearance and
condition and, in particular, for reversing, reducing or preventing the visible signs of
wrinkled, aged and/or photo damaged skin has grown enormously. Therefore consumers
are on continuous look out for cosmetic solutions to their skin appearance problems.
Smooth, soft and glowing, wrinkle free skin with even skin tone and color is thus
desired by all consumers who use personal care compositions for their skin.
To provide these benefits, manufacturers from around the world have tried many
approaches. One very commonly used approach is to control the appearance of the skin
by scavenging free radicals. Environmental insults to human skin by ultraviolet (UV)
radiation, as well as by cigarette smoke and air pollution, generates reactive oxygen

intermediates that contribute to both acute and chronic skin damage. For example,
immediately after overexposure to sunlight, an erythemal response is induced that is
associated with epidermal inflammatory oxidative reactions. Moreover, in terms of
chronic exposure, the involvement of oxygen free radicals has also been implicated in
actinic skin damage that manifests itself in elastosis, collagen disorganization and most
notably in the appearance of wrinkles.
Another approach is by adding chemical compounds of natural or synthetic origin to
cosmetic products that targets different stages of damage causing/ reversing
mechanisms such as increasing extracellular matrix (ECM) proteins, decreasing
proteolytic activity of enzymes responsible for ECM breakdown such as inhibiting
matrix metalloproteases, increasing growth factors, etc. These in turn reverse, treat or
delay the visible signs of chronoageing and photo-ageing skin such as wrinkles, lines,
sagging, hyper-pigmentation and age spots.
Aging of skin results from a combination of intrinsic factors, e.g., genetic factors, and
extrinsic factors, e.g., environmental factors such as sun damage and smoking. Aging of
skin affects cells and tissues at the microscopic level, and manifests outwardly as
various signs, e.g., wrinkles, dryness, roughness, dullness, brown/dark/age spots, and
sagging of the skin.
Although a number of anti-aging skin care products are commercially available,
synthetic ingredients in many of these products may be harsh, irritating or even harmful
to the skin with use over time. Consumers are seeking skin care products that are not
only natural but also effective with little or no side effects.
Thus there is a need to provide a novel personal care composition that exhibits both free
radical scavenging (anti-oxidant) activity and delays ageing by any of the processes
such as decreasing proteolytic activity, increasing ECM proteins or inhibiting matrix
metalloproteases.
Objects of invention

An object of the present invention is to overcome the drawbacks of the prior art.
Another object of the present invention is to provide a personal care composition
comprising the extract of Tecomella undulata.
Yet another object of the present invention is to provide a personal care composition
comprising the extract of Tecomella undulata with demonstrably better anti-oxidant and
anti-ageing activity.
Summary of the present invention
An aspect of the present invention is to provide a personal care composition comprising
extract of Tecomella undulata and cosmetically acceptable excipients wherein said
extract ranges from 0.00001 % to about 10 % by weight.
Brief Description of the Accompanying Drawings
Figure 1 illustrates that if the concentration of extract is below 0.01% (below 100 ppm)
the percent MMP inhibition (anti-aging effect) value is on the negative side.
Figure 2 illustrates top view the formulation with different concentration of T. undulata
extract (with coloring pigment) placed in a beaker.
Figure 3 illustrates determination of anti-oxidant activity of T. undulata through DPPH
assay. It was observed that the plant extract showed a potent free radical scavenging
activity from 90.4% to 3.86% when tested at concentration range of 10,000 ppm to 0.1
ppm.
Figure 4 illustrates determination of anti-aging activity of T. undulata through MMP
inhibition assay. It was observed that the plant extract showed a showed MMP
inhibition of 101.04% and 20.34% when tested at concentration range of 1000 ppm
to 100 ppm respectively.
Figure 5 illustrates comparative data illustrating the criticality of using stem bark
extract to obtain desired results.

Detailed Description of the Invention
The inventors have surprisingly found that stem-bark extract solution of Tecomella
undulata can reduce signs of skin ageing and exhibits anti-oxidant activity when applied
topically.
Accordingly the present invention provides a personal care composition comprising
Tecomella undulata extract as the active ingredient along with cosmetically acceptable
ingredients. The present invention employs the extract from the stem-bark region.
The term 'personal care composition' as used herein is meant to include a composition
for topical application to skin of humans. Such a composition is preferably a leave-on
composition. It includes any product applied to a human body for getting improved
appearance, cleansing, odour control, photo protection or general aesthetics and is
especially useful for providing improved appearance of human skin. The composition of
the present invention can be in any form such as a liquid, lotion, cream, or gel.
In the present composition, Tecomella undulata extract is present in an amount of about
0.00001% to about 10% by weight of the composition, preferably, from about 0.001%
to about 10% by wt., more preferably about 0.001% to about 7% by wt., and even more
preferably about 0.01% to about 5% by wt.
The cosmetically acceptable excipients can be selected from silicones, vitamin and/or
derivatives thereof, polyols, vehicles, structurants, emollient/s, gelling agent/s, a
thickening agent/s, a hydrophilic or hydrophobic polymer, an emulsifying agent/s,
alcohol/s etc. Examples of these ingredients include but are not limited to such
substances as binders, emollients, preservatives (such as methyl paraben), colorants,
perfumes, skin-lightening agents, antimicrobials and the like.
The polyols used are selected from glycerol, ethylene glycol, propylene glycol,
pentaerythritol, diglycerol, polyglycerol, their derivatives and combinations thereof. In a
preferred embodiment the invention relates to compositions wherein the concentrations
of polyol compounds varies from about 0.1% to 10.0% by wt; preferably between 0.5%
and 5.0 %by wt, most preferably between 1.0% by wt. and 4.0% by wt.

The silicone used in the invention is selected from linear, branched, cross linked,
silicone oils, volatile and non volatile silicones such as dimethicone copolyol,
dimethylpolysiloxane, diethylpolysiloxane, high molecular weight dimethicone, mixed
C1-C30 alkyl polysiloxane, phenyl dimethicone, dimethiconol, cyclopentasiloxane,
dimethicone, dimethiconol, mixed C1-C30 alkyl polysiloxane, and mixtures thereof.
The concentration of silicones is about 0.01% to 5.0%; preferably about 0.1% to 3.0%
by wt, more preferably about 0.5 to 2.0% by wt.
The vitamin used in the composition is selected from a group comprising vitamin A,
vitamin B (1-12), vitamin C, vitamin D (2-4), vitamin E, vitamin K, their derivatives,
such as acetates, propionates, palmitates, phosphates, alone on in combinations thereof.
The concentrations of the vitamin and/or its derivative(s) may be about 0.01 to 5.0% by
wt. preferably about 0.05% by wt. and 3.0% by wt., and more preferably between 0.5%
by wt and 2.0% by wt.
Emulsifying agents which may be optionally added to the compositions of the present
invention include but are not restricted to oxyalkylenated fatty acid esters of polyols, for
example polyethylene glycol stearates, for instance PEG-100 stearate, PEG-50 stearate
and PEG-40 stearate; and mixtures thereof, mixture of glyceryl monostearate and of
polyethylene glycol stearate (100 EO) (Simulsol 165), oxyalkylenated fatty acid esters
of sorbitan comprising, for example, from 20 to 100 EO such as Tween 20 or Tween 60,
oxyalkylenated (oxyethylenated and/or oxypropylenated) fatty alcohol ethers;
alkoxylated or non-alkoxylated sugar esters, such as sucrose stearate and PEG-20
methylglucose sesquistearate; sorbitan esters such as the sorbitan palmitate (Span 40),
esters of diacid and of fatty alcohol, such as dimyristyl tartrate; mixtures of these
emulsifiers, for instance the mixture of glyceryl stearate and of PEG-100 stearate
(Arlacel 165), and mixtures comprising these emulsifiers, such as the mixture of
dimyristyl tartrate, cetearyl alcohol, Pareth-7 and PEG-25 laureth-25, (Cosmacol PSE),
steareth - 2, steareth 21, PPG-15 stearyl ether, cetearyl alcohol, cetyl alcohol. The

concentrations of the emulsifying agents may be about 0.1% by wt. to about 8.0% by
wt; preferably about 0.4% by wt. to about 4.0%) by wt.
Thickeners which may be used in the instant invention include but are not restricted to
alkyloamides, carbomer 934,940,941,960,961, polyamide, polysaccharides, acrylates,
gelatin, gums, and magnesium aluminium silicates, ozocarite, paraffin, tragacanth,
sodium alginate, Tinovis ADM, ammonium Acryloyl - dimethyltaurate / VP
Copolymer/Carbomer, Hydroxyethyl Acrylate / Sodium Acryloyldimethyl Taurate
Copolymer (and) Isohexadecane (and) Polysorbate 60; Magnesium sulfate;
Acrylates/C 10-30 Alkyl Acrylate Cross-polymer; Potassium Cetyl Phosphate (and)
Hydrogenated Palm Glycerides and the like. The concentrations of the thickeners may
be about 0.1% to about 2%; preferably about 0.5% by wt to about 1% by wt.
Structurants which may be used in compositions of the present invention include those
materials which are well known in the art and include fatty acids, fatty alcohols, fatty
acid esters, and fatty acid amides, having fatty chains of from 8 to 30 carbons atoms.
Preferably the structurant used is stearic acid. The concentrations of the structurants
may be about 0.1 % by wt. to about 8.0% by wt.; preferably about 0.4% by wt. to about
4.0% by wt.
Various emollients as are known to a person skilled in the art and as are available in the
market can be employed as an optional constituent in the instant invention. These
include but are not restricted to the group consisting of lanolin, octyl dodecanol, hexyl
decanol, oleyl alcohol, decyl oleate, isopropyl stearate, isopropyl palmitate, isopropyl
myristate, hexyl laureate, dioctyl cyclohexane, PPG-15 stearyl ether, isohexadecane,
stearic acid, cetyl alcohol, mineral oil etc. The concentrations of the emollients may be
about 0.P/o by wt to 10% by wt; preferably about 1% by wt and 5% by wt.
Various chelating agents may be used as optional constituents of the instant invention.
These may be selected from a group consisting of but not limited to Dimercaptosuccinic
acid (DMSA), Dimercapto-propane sulfonate (DMPS), Alpha lipoic acid (ALA),
Calcium disodium versante (CaNa2-EDTA), Disodium EDTA, Dimercaprol (BAL). The

concentrations of the various chelating agents may be about 0.1% by wt. to 1% by wt;
preferably about 0.2% by wt to about 0.5% by wt.
Various cosmetically and dermatologically suitable preservatives may be added to the
instant composition. These may be selected from the group consisting of but not
restricted to 2-phenoxyethanol, para-hydroxybenzoic acid esters, also known as
parabens, for instance methyl para-hydroxybenzoate (methyl paraben), ethyl para-
hydroxybenzoate (ethyl paraben) and propyl para-hydroxybenzoate (propyl paraben)
and mixtures thereof; formaldehyde-releasing agents, for instance imidazolidinylurea or
diazolidinylurea; haloalkynyl carbamates, for instance 3-iodo-2-propynyl butyl
carbamate (IPBC); caprylyl glycol, also known as 1,2-octanediol; sodium benzoate; N-
(3-chloroallyl)-hexaminium chloride (or Quaternium-15); polyhexamethylene biguanide
hydrochloride (CTFA name: polyaminopropyl biguanide); alkyltrimethylammonium
bromides, for instance dodecyltrimethylammonium bromide,
myristyltrimethylammonium bromide and hexadecyltrimethylammonium bromide, and
mixtures thereof. The concentrations of the various cosmetically and dermatologically
suitable preservatives may be about 0.05 to 1.5% by wt; preferably about 0.1% and 1%
by wt.
Anti-ageing active agents which may optionally added to the compositions of the instant
invention may be chosen from free-radical scavengers, keratolytic agents, vitamins,
anti-elastase and anti-collagenase agents, proteins, fatty acid derivatives, steroids, trace
elements, bleaching agents, algal and plankton extracts, enzymes and coenzymes,
flavonoids, ceramides, tensioning agents and muscle relaxants, and mixtures thereof.
The concentrations of the Anti-ageing active agents may be about 0.001% to about 10
wt %, preferably from about 0.01% to about 5 wt %.
Free-radical scavengers and antioxidants which may be optionally added to the
compositions of the instant invention include but are not restricted to phosphonic acid
derivatives such as ethylenediaminetetra (methylenephosphonic acid), methylene
phosphonic acid, methylenephosphonic acid and salts thereof, in particular the sodium

salts thereof; ethylenediaminetetraacetic acid and its salts, such as the sodium salt;
guanosine; superoxide dismutase; tocopherol (vitamin E) and its derivatives (acetate);
ethoxyquine; lactoferrin; lactoperoxidase, and nitroxide derivatives; superoxide
dismutases; glutathione peroxidase; plant extracts with free-radical-scavenging activity,
such as the aqueous extract of wheatgerm (Detoxiline), green tea, and mixtures thereof.
The concentrations of the Free-radical scavengers and antioxidants may be about 0.001
wt% to about 2 wt %, preferably from about 0.01 wt% to about 1 wt %.
Hydrophillic /hydrophobic polymers may be classified under the broad class of
thickeners. These may also alternatively be called as stabilizers.
Skin-lightening agents can be chosen from a group comprising, but are not limited to,
tyrosinase inhibitors, melanin-inhibiting agents, melanosome transfer inhibiting agents
including PAR-2 antagonists, exfoliants, sunscreens, retinoids, antioxidants,
Tranexamic acid, skin bleaching agents, allantoin, opacifiers, talcs and silicas, zinc
salts, and the like, and other agents as described in Solano et al. Pigment Cell Res. 2006,
19 (550-571). Examples of suitable tyrosinase inhibitors include but, are not limited to,
Vitamin C and its derivatives, Vitamin E and its derivatives, Kojic Acid, Arbutin,
resorcinols, hydroquinone, Flavones e.g. Licorice flavanoids, Licorice root extract,
Mulberry root extract, Dioscorea Coposita root extract, Saxifraga extract and the like,
Ellagic acid, Salicylates and derivatives, Glucosamine and derivatives, Fullerene,
Hinokitiol, Dioic acid, Acetyl glucosamine, Magnolignane, combinations of two or
more thereof, and the like. Examples of vitamin C derivatives include, but are not
limited to, ascorbic acid and salts, Ascorbic Acid-2-Glucoside, sodium ascorbyl
phosphate, magnesium ascorbyl phosphate, and natural extract enriched in vitamin C.
Examples of vitamin E derivatives include, but are not limited to, alpha-tocopherol,
beta, tocopherol, gamma-tocopherol, delta-tocopherol, alpha-tocotrienol, beta-
tocotrienol, gamma-tocotrienol, delta-tocotrienol and mixtures thereof, tocopherol
acetate, tocopherol phosphate and natural extracts enriched in vitamin E derivatives.
Examples of resorcinol derivatives include, but are not limited to, resorcinol, 4-
substituted resorcinols like 4alkylresorcinols such as 4-butyresorcinol (rucinol), 4-

hexylresorcinol, phenylethyl resorcinol, l(2,4-dihydroxyphenyl)-3-(2,4-dimethoxy-3-
methylphenyl)-Propane and the like and natural extracts enriched in resorcinols.
Examples of salicylates include, but are not limited to, salicylic acid, acetylsalicylic
acid, 4-methoxysalicylic acid and their salts. In certain preferred embodiments, the
tyrosinase inhibitors include a 4-substituted resorcinol, a vitamin C derivative, or a
vitamin E derivative. In more preferred embodiments, the tyrosinase inhibitor comprises
Phenylethyl resorcinol, 4-hexyl resorcinol, or ascorbyl-2-glucoside.
Examples of suitable melanin-degradation agents include, but are not limited to,
peroxides and enzymes such as peroxidases and ligninases. In certain preferred
embodiments, the melanin-inhibiting agents include peroxide or ligninase.
Examples of suitable melanosome transfer inhibiting agents includes PAR-2 antagonists
such as soy trypsin inhibitor or Bowman-Birk Inhibitor, Vitamin B3 and derivatives
such as Niacinamide, Essential soy, Whole Soy, Soy extract.
In a preferred embodiment, the melanosome transfer inhibiting agents is a soy extract or
niacinamide.
Examples of exfoliants include, but are not limited to, alpha-hydroxy acids such as
lactic acid, glycolic acid, malic acid, tartaric acid, citric acid, or any combination of any
of the foregoing, beta-hydroxy acids such as salicylic acid, polyhydroxy acids such as
lactobionic acid and gluconic acid, and mechanical exfoliation such as
microdermabrasion. In certain preferred embodiments, the exfoliant include glycolic
acid or salicylic acid.
Examples of sunscreens include, but are not limited to, avobenzone (Parsol 1789),
bisdisulizole disodium (Neo Heliopan AP), diethylamino hydroxybenzoyl hexyl
benzoate (Uvinul A Plus), ecamsule (Mexoryl SX), methyl anthranilate, 4-
aminobenzoic acid (PABA), cinoxate, ethylhexyl triazone (Uvinul T 150), homosalate,
4-methylbenzylidene camphor (Parsol 5000), octyl methoxycinnamate (Octinoxate),
octyl salicylate (Octisalate), padimate 0 (Escalol 507), phenylbenzimidazole sulfonic

acid (Ensulizole), polysilicone-15 (Parsol SLX), trolamine salicylate, Bemotrizinol
(Tinosorb S), benzophenones 1-12, dioxybenzone, drometrizole trisiloxane (Mexoryl
XL), iscotrizinol (Uvasorb HEB), octocrylene, oxybenzone (Eusolex 4360),
sulisobenzone, bisoctrizole (Tinosorb M), titanium dioxide, zinc oxide, and the like.
Examples of retinoids include, but are not limited to, retinol, retinaldehyde, retinoic
acid, retinyl palmitate, isotretinoin, tazarotene, bexarotene and Adapalene. In certain
preferred embodiments, the retinoid is retinol.
The additional cosmetically active agent may be present in a composition in any
suitable amount, for example, in an amount of from about 0.0001% by wt to about 20%
by weight of the composition, e.g., about 0.001% by wt to about 10% by wt such as
about 0.01% by wt to about 5% by wt. In certain preferred embodiments, in an amount
of 0.1% by wt to 5% by wt and in other preferred embodiments from 1% by wt to 2%
by wt.
The composition of the invention may utilize a fragrance composition comprising a
blend of essential oils and synthetic aroma compounds. The blend is often diluted with a
carrier like propylene glycol, vegetable oil, or mineral oil. Some examples of synthetic
aroma compound which may be optionally added to the compositions of the instant
invention include but are not restricted to benzaldehyde, citral, vanillin, ethyl acetate,
fructone, octyl acetate, pentyl butanoate, pentyl pentanoate, methyl salicylate, isoamyl
acetate, limonene, citronellol, and mixtures thereof. The amount of fragrance ranges
from 0.2 to 2 wt / wt%.
Emotives that may be used in the present invention include but are not limited to
mentha, jasmine, camphor, white cedar, bitter orange peel, ryu, turpentine, cinnamon,
bergamot, citrus unshiu, calamus, pine, lavender, bay, clove, hiba, eucalyptus, lemon,
starflower, thyme, peppermint, rose, sage, sesame, ginger, basil, juniper, lemon grass,
rosemary, rosewood, avocado, grape, grapeseed, myrrh, cucumber, watercress,
calendula, elder flower, geranium, linden blossom, amaranth, seaweed, ginko, ginseng,
carrot, guarana, tea tree, jojoba, comfrey, oatmeal, cocoa, neroli, vanilla, green tea,

penny royal, aloe vera, menthol, cineole, eugenol, citral, citronelle, borneol, linalool,
geraniol, evening primrose, camphor, thymol, spirantol, penene, limonene, rose, lemon
grass, peach, honey, almond oil, olive oil, shea butter, olive butter, bearberry, black
currant, rosemary, blue lotus, white lily, and terpenoid oils.
The amount of emotives present in the personal care composition ranges from 0.0001%
by wt to 0.5% by wt.
Colourants that may be included in the composition of the present invention including,
but not limited to, inorganic, nitroso, monoazo, disazo, carotenoid, triphenyl methane,
triaryl methane, xanthene, quinoline, oxazine, azine, anthraquinone, indigoid,
thionindigoid, quinacridone, phthalocianine, botanical and natural colors, and
combinations thereof.
The amount of colorants ranges from 0.0001 to 0.0005 wt/wt%.
Various cosmetically acceptable vehicles may be used for the preparation of
compositions as per the instant invention. These may be selected from the group
consisting of but not restricted to water, alcohols, oils and combinations thereof.
Accordingly, when the surface contemplated is skin, the composition of this invention
may contain ingredients, which are added to creams, lotions, ointments, gels or
medicaments, which are physiologically acceptable to skin and may not contain
ingredients, which will reverse or retard the action of anti-oxidants/ anti-ageing
agent(s).
The inventors have surprisingly found out that personal care compositions comprising
100 ppm to 1000 ppm of stem-bark extract of Tecomella undulata as the active exhibits
anti-ageing and antioxidant activity.
The composition may be in the form of conventional skin-care products such as a skin
creams, lotions, gels, serums, etc.

In another aspect of the present invention there is provided a process of preparation of
the powdered form of Tecomella undulata extract.
• Firstly the preparation of the plant extract is done by the addition of stem bark
powder to distilled water.
• The amount of stem bark powder of Tecomella undulata used in the process for
preparation of the personal care composition is 10gms. This powder is added to
100 mL distilled water.
• The step is followed by heating the plant extract for a specified temperature. The
concentrated plant extract is then distilled and then filtered.
• The plant extract disclosed in the second step in the process listed above is
steamed at a temperature ranging from 80°C-100°C followed by percolation or
extraction for a minute, for four cycles, each cycle for 4 hours.
• The process of distilling takes about 10-12 hours and the filtering is done using a
40-60 mesh.
• The liquid paste is then dried under vacuum at a high temperature, obtaining the
dry powder of Tecomella undulata extract. The temperature at which the
vacuum drying takes place ranges from 80°C to 100°C for a time ranging from 2
hrs to 4 hours.
In another embodiment of the present invention there is provided a process for the
preparation of the personal care composition. The process comprises steps of:
a) weighing water soluble ingredients along with thickeners and making a
neutralized premix of the same in water;
b) oil soluble ingredients are then weighed and melted to obtain a molten oil phase;
c) the neutralized premix and the molten oil phase are homogenized together and
an emulsion formulation is formed; and
d) natural extract of Tecomella undulata along with emotives, colorants and
fragrance are added to the emulsion and the desired personal care formulation of
the present invention is obtained.

The term "water phase" is defined as and includes those cosmetically suitable additives
as are hydrophilic and/or soluble in water. These additives may be selected from a
group comprising Di sodium EDTA, magnesium sulphate, acrylates/C 10-30 Alkyl
Acrylate Crosspolymer and ammonium Acryloyl - dimethyltaurate / VP Copolymer/
Carbomer or any combination of mentioned component(s) may also be used.
Other agents may also be added to the cosmetic composition of the present invention
have been stated above please. Polymers / thickeners (acrylates/C 10-30 Alkyl Acrylate
Crosspolymer and ammonium Acryloyl - dimethyltaurate / VP Copolymer/Carbomer),
Chelating agents / chelatants (Di sodium EDTA) as specified in the example are merely
illustrative. These may be suitably replaced with other agents, as stated above, by a
person skilled in the art and may therefore please not be considered limiting to the scope
of the invention.
Thickeners which may be used in the instant invention include but are not restricted to
alkyloamides, carbomer 934,940,941,960,961, polyamide, polysaccharides, acrylates,
gelatin, gums, and magnesium aluminium silicates, ozocarite, paraffin, tragacanth,
sodium alginate, Tinovis ADM, ammonium Acryloyl - dimethyltaurate / VP
Copolymer/Carbomer, Hydroxyethyl Acrylate / Sodium Acryloyldimethyl Taurate
Copolymer (and) Isohexadecane (and) Polysorbate 60; Magnesium sulfate;
Acrylates/C 10-30 Alkyl Acrylate Cross-polymer; Potassium Cetyl Phosphate (and)
Hydrogenated Palm Glycerides and the like.
The concentrations of the thickeners may be about 0.1% to about 2%; preferably about
0.5% by wt to about 1% by wt.
The composition of the present invention may further include one or more neutralizers,
such as, for example, strong and weak bases. Any suitable neutralizer can be selected, as
will be appreciated by one of ordinary skill in the art. Exemplary neutralizers suitable
for use in the compositions of the present invention included sodium hydroxide,
potassium hydroxide, ammonium hydroxide, diethanolamine, triethanolamine, 2-
dimethylamino-2-methyl-l-propanol (DAMP), 2-aminomethyl-lpropanol (aminomethyl

propanol) (AMP), and the like, or combinations thereof. The neutralizer, if present, may
be provided in any amount, e.g., an amount sufficient to achieve a desired pH for the
composition. In this respect, the composition preferably has a pH of from about 4-9,
more preferably, from about 5-8, and still more preferably from about 5.5-7. Typically,
the neutralizer may be present in an amount of from about 0.01% -10% by weight of the
composition.
The term "oil phase" is defined as and includes those cosmetically suitable additives as
are soluble in oil. The oil phase of the skin care composition is selected from a group
comprising of C12-15 Alkyl Benzoate, Potassium Cetyl Phosphate (and) Hydrogenated
Palm Glycerides, Cetostearyl alcohol and Glyceryl Stearate and PEG-100 Stearate,
Hydroxyethyl Acrylate / Sodium AcryloyldimethylTaurate Copolymer (and)
Isohexadecane (and) Polysorbate 60. or any combination of mentioned component(s)
may also be used.
Emotives that may be used in the present invention include but are not limited to
mentha, jasmine, camphor, white cedar, bitter orange peel, ryu, turpentine, cinnamon,
bergamot, citrus unshiu, calamus, pine, lavender, bay, clove, hiba, eucalyptus, lemon,
starflower, thyme, peppermint, rose, sage, sesame, ginger, basil, juniper, lemon grass,
rosemary, rosewood, avocado, grape, grapeseed, myrrh, cucumber, watercress,
calendula, elder flower, geranium, linden blossom, amaranth, seaweed, ginko, ginseng,
carrot, guarana, tea tree, jojoba, comfrey, oatmeal, cocoa, neroli, vanilla, green tea,
penny royal, aloe vera, menthol, cineole, eugenol, citral, citronelle, borneol, linalool,
geraniol, evening primrose, camphor, thymol, spirantol, penene, limonene, rose, lemon
grass, peach, honey, almond oil, olive oil, shea butter, olive butter, bearberry, black
currant, rosemary, blue lotus, white lily, and terpenoid oils.
The amount of emotives present in the personal care composition ranges from 0.0001 to
0.5.
Colourants that may be included in the composition of the present invention including,
but not limited to, inorganic, nitroso, monoazo, disazo, carotenoid, triphenyl methane,

triaryl methane, xanthene, quinoline, oxazine, azine, anthraquinone, indigoid,
thionindigoid, quinacridone, phthalocianine, botanical and natural colors, and
combinations thereof.
The amount of colorants ranges from 0.0001 to 0.0005wt/wt%.
The composition of the invention may utilize a fragrance composition comprising a
blend of essential oils and synthetic aroma compounds. The blend is often diluted with a
carrier like propylene glycol, vegetable oil, or mineral oil. Some examples of synthetic
aroma compound which may be optionally added to the compositions of the instant
invention include but are not restricted to benzaldehyde, citral, vanillin, ethyl acetate,
fructone, octyl acetate, pentyl butanoate, pentyl pentanoate, methyl salicylate, isoamyl
acetate, limonene, citronellol, and mixtures thereof. The amount of fragrance ranges
from 0.2 to 2 wt / wt%.
Yet another embodiment of the present invention provides a cosmetic method of
providing at least one skin care benefit selected from: skin ageing, antioxidant effect;
the method comprising applying to the skin the personal care composition prepared
according to the present invention.
The present invention is now illustrated by way of non limiting examples.
Example 1: Skin cream composition comprising Tecomella undulata stem-bark
extract

Example3: Preparation of Tecomella undulata stem-bark extracts (10% aqueous
extract)
One gram of the dried stem bark powder was added to 10 ml of distilled water to
prepare 10% extract. It was then heated at 80°C - 100°C for 20 minutes. The
concentrated extract was distilled for 12 hours and filtered (using filter of 40-60 mesh).
The liquid paste was dried under vacuum dry at 80°C for 4 hours to obtain extract of
Tecomella undulata in dry powder format.
Example 4: Determination of anti-oxidant activity of T. undulata through DPPH
Assay
DPPH is one of the reliable methods for estimating antioxidant activity. The molecule
of 2, 2, diphenyl-1-picryl-hydrazyl (DPPH) is characterized as a stable free radical by
virtue of the derealization of the spare electron over the molecule as a whole so that the
molecules do not dimerise. The derealization also gives rise to deep violet color;
characterized by an absorption band at around 520 nm.lO ul of T. undulata extract of
different concentrations was added to 190 uL of DPPH solution. L-Ascorbic acid was
used as a positive control. Absorbance was read against a suitable blank at 520 nm. The
percent free radical scavenging activity was determined. It was found that T. undulata
extract possesses potent anti-oxidant activity (Fig 3).
Example 5: Determination of MMP inhibition activity of T. undulata through
MMP inhibition assay.
MMP (matrix metalloproteinase) inhibition assay aims to measure the protease activity
of MMP-1 using thiopeptide as a chromogenic substrate. The MMP cleavage site
peptide bond is replaced by a thioester bond in thiopeptide. Hydrolysis of this bond by
an MMP produces a sulfhydryl group, which reacts with Ellman's reagent to form 2-
nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm. 20 uL of

T. undulata extract of different concentrations was incubated with 20 uL of MMP-1
enzyme, 10 uL of substrate and 50 uL of assay buffer. The reaction kinetics was read at
412 A against a suitable blank for 30 minutes. It was found that T. undulata possesses a
higher MMP inhibition activity when compared to standard inhibitor N-Isobutyl-N-(4-
methoxyphenylsulfonyl) glycyl hydroxamic acid (NNGH) (Fig 4).
Example 6: comparative data illustrating the criticality of using stem bark extract
to obtain desired results (Figure 5)

The anti-oxidant activity of the stem bark extract of Tecomella undulata was compared
with the seed extract of the same plant. It was observed that the seed extract showed
significantly lower levels of anti-oxidant activity as compared to the stem bark extract
when tested at concentrations between 10, 000 ppm to 1 ppm as illustrated in the table
above.
Example 7: Personal care formulations (skin cream) comprising Tecomella
undulata extract:



Process for preparation of cream:
• Weighing Xanthan gum, Disodium EDTA, Magnesium sulfate, Phenoxyethanol,
Ethylhexyl Glycerin, Ammonium Acryloyl - dimethyltaurate / VP Copolymer
Acrylates/C 10-30 Alkyl Acrylate Cross-polymer, and made a premix of the same in
water by heating them to 75 - 85° C for about 15-20 minutes;
• Adding Triethanolamine to the premix to neutralize the same to obtain a neutralized
premix;
• Weighing C12-15 Alkyl Benzoate, Potassium Cetyl Phosphate (and) Hydrogenated
Palm Glycerides, Cetostearyl alcohol, Glyceryl Stearate (and) PEG-100 Stearate,
Hydroxyethyl Acrylate / Sodium AcryloyldimethylTaurate Copolymer (and)
Isohexadecane (and) Polysorbate 60 and melted the same at 75 - 80 °C for 15 - 20
minutes to obtain a molten oil phase;
• Homogenizing neutralized premix with molten oil phase of step 3 at 55- 60 °C for
15 minutes to obtain an emulsion formulation;
• Adding natural extract of Tecomella undulata and fragrance to the emulsion to
obtain desired cream formulation of the present invention.


Process for preparation of serum:
• Weighing EDTA, Glycerin, butylene glycol and mixed it with water to form a
premix;
• Solubilising Galaxy 2932, DL-a-Tocopherol Acetate and Allantoin in Croduret 40
by heating at 40 ° C for 10 mins. The mixture was brought to room temperature
before addition to the premix of step 1 to form solubilized premix;
• Adding Amaze XT , d-Panthenol, Hydramol TM TGL ester to solubilized premix
and mixed for about 15-20 minutes; and
• Dow Corning 2501 cosmetic wax was added to mixture followed by phenoxetol,
natural extract and fragrance to obtain serum formulation of the present formulation.
Example 9: Personal care formulations (lotion) comprising Tecomella undulata
extract:



Process for the preparation of lotion:
• Weighing Glycerine, Propylene Glycol, Disodium EDTA, Phenoxyethanol,
Hydrovance, PEG/PPG-8/3 Di-isostearate, silicones, Acrylates/ C10-30 alkyl
acrylate crosspolymer, Carbomer, Acrylates/ Acrylamide copolymer (and) mineral
oil (and) polysorbate 85 (and) aqua and made a premix of the same in water by
heating them to 75 - 85° C for about 15-20 minutes;
• Adding Triethanolamine to the premix to neutralize the same to obtain a neutralized
premix;

• Weighing Octylmethoxycinnamate , Steareth-21, Steareth-2, Stearic acid, Cetyl
Alcohol, Tocopheryl Acetate, C12-15 Alkyl Benzoate, Potassium Cetyl Phosphate
(and) Hydrogenated Palm Glycerides, Cetostearyl alcohol, Glyceryl Stearate (and)
PEG-100 Stearate, Hydroxy ethyl Acrylate / Sodium AcryloyldimethylTaurate
Copolymer (and) Isohexadecane (and) Polysorbate 60 and melted the same at 75 -
80 °C for 15 - 20 minutes to obtain a molten oil phase;
• Homogenizing neutralized premix with molten oil phase at 55- 60 °C for 15 minutes
to obtain an emulsion formulation;
• Adding natural extract of Tecomella undulata and fragrance to the emulsion to
obtain desired cream formulation of the present invention.

WE CLAIM:
1. A personal care composition comprising extract of Tecomella undulata and
cosmetically acceptable excipients.
2. The personal care composition as claimed in claim 1, wherein said extract is
stem bark extract of Tecomella undulata.
3. The personal care composition as claimed in claim 1, wherein said extract
ranges from 0.001% to 10% by wt.
4. The personal care composition as claimed in claim 1, wherein said extract
ranges from 0.001% to 7% by wt.
5. The personal care composition as claimed in claim 1, wherein said cosmetically
acceptable excipients are selected from silicones, vitamin and/or derivatives
thereof, polyols, vehicles, structurants, emollient/s, gelling agent/s, thickeners,
an emulsifying agent/s, chelating agent, preservative, skin lightening agents,
antimicrobials, neutralizers, anti ageing active agent and free radical scavenging
agents and mixtures thereof.
6. The personal care composition as claimed in claim 1, wherein said composition
is selected from lotion, serum and cream.

ABSTRACT

A personal care composition comprising the extracts of Tecomella undulata and
cosmetically acceptable excipients is provided. The composition of the present
invention aims at providing skin care benefits like skin ageing, antioxidant effect etc.