CLIAMS:1. An anti-aging personal care composition comprising: 0.001% to 3.0 % by weight naringenin and 0.001% to 3.0 % by weight naringin, wherein said naringenin and naringin are present in a weight ratio from 1: 3 to 3:1.
2. The anti-aging personal care composition as claimed in claim 1, said composition comprising further cosmetically acceptable excipients selected from structurants, emulsifiers, humectants, actives, neutralizers, emollients, feel enhancers, rheology modifiers, bioactives, preservatives, fragrances and mixtures thereof.
3. The anti-aging personal care composition as claimed in claim 1, wherein said composition is prepared in the form of lotions, serums, oils, sprays, milks, gels and gel-creams.
,TagSPECI:FILED OF INVENTION
The present invention relates to personal care compositions. More specifically it relates to anti-aging composition comprising natural product.

BACKGROUND OF INVENTION
Skin is subject to deterioration through dermatological disorders, environmental abuse (wind, air conditioning, and central heating) or through the normal aging process (chronoageing) which may be accelerated by exposure of skin to sun (photoageing).
Since thousands of years people have been conscious about need of skin care for various reasons, including cleanliness, comfort, beauty, and prevention of wrinkles, aging and protection against the sun. Research has confirmed the benefits of proper skin care through the use of products that contain anti-aging ingredients, primarily actives, anti-oxidants and sunscreens.
Skin care formulations using various extracts and actives for curing skin conditions are known in various prior art. For example, US4297348 discloses about therapeutic composition comprising naringin and naringenin as major components. The therapeutically effective composition comprises a) from about 0.1% to about 5% by weight naringin; b) from about 0.1% to about 10% by weight naringenin; c) from about 0.05% to about 5% by weight of an antimicrobially active partially hydrolyzed naringin; d) and few other components. However the composition of US4297348 is for preventing acne and not for curing age related skin conditions.
US6596761 provides a cosmetic and dermatological composition comprising one or more compounds selected from the group consisting of the various flavonoids including naringenin, naringin for the prophylactic treatment of stinging or the non-specific itching of the skin.
US8530426 discloses about cosmetic or dermopharmaceutical composition comprising a UGT inducer and dermatologically acceptable carrier to protect or enhance the state of the skin and to treat imperfections of the skin. The UGT inducer is selected from naringenin and naringin amongst others.
Thus the prior art compositions which comprises of flavonoids such as naringenin, naringin relate to therapeutic anti acne compositions which essentially effective for anti-microbial activity. Further the prior art discloses high amounts of actives such as from 0.1 to 10% by weight and also does not teach use of specific ratio of two actives Naringenin and naringin. There is always need for more efficient easily available actives for skin care compositions that enhance appearance and provide anti ageing effect. The demand for cosmetic compositions and cosmetic methods for anti-aging skin products has grown enormously. Accordingly the present invention provides anti-aging products with combination of naringin and naringenin in a specific ratio where the actives are present in low amount.

OBJECTIVES OF THE PRESENT INVENTION
It is an object of the present invention to overcome the drawbacks of the prior art.
It is another object of the present invention to provide a cosmetic composition containing naringenin and naringin to provide good anti-aging effect.

SUMMARY OF THE PRESENT INVENTION
According to the present invention there is provided an anti-aging personal care composition comprising: 0.001% to 3.0 % by weight Naringenin and 0.001% to 3 % by weight Naringin, wherein said Naringenin and Naringin are present in a weight ratio from 1:3 to 3:1.

DETAILED DESCRIPTION OF PRESENT INVENTION
The present invention provides a personal care composition containing naringenin and naringin which possesses anti-aging property. The present inventors have surprisingly found that when naringenin and naringin are combined at ratios from 1:3 to 3:1 it provides a synergistic anti-aging effect.
The present invention provides a holistic approach to address aging rather than conventional approaches wherein multiple pathways contributing to aging are studied, targets identified and molecules working towards particular targets are combined to obtain an anti-aging product.
The present inventors have used Caenorhabditis elegans (C. elegans), which is a simple multicellular organism, as a model system for studying the aging process. It is known that actives/ molecules that improve the life span of Caenorhabditis elegans is used to develop anti-aging products.
The present inventors have found that the combination of naringenin and naringin at a certain weight ratio synergistically improves the life span of Caenorhabditis elegans.
The present invention thus provides an anti aging composition comprising 0.001% to 3.0 % by weight naringenin and 0.001% to 3.0 % by weight naringin, wherein said naringenin : said naringin is in a weight ratio from 1:3 to 3:1.
The personal care compositions of the present invention may further comprise cosmetically acceptable excipients selected from structurants, chelants, emollients, diluents, humectants, actives, neutralizers, pH modifiers, preservatives and fragrances. The list of structurants employed may include but not be limited to starch, modified starch, guar gum, carbomers, silicone based polymers, acrylates and cellulose based polymers. Emollients that may be employed include silicone oils and modifications thereof such as linear and cyclic polydimethylsiloxanes, volatile silicones, esters such as cetyloctanoate, myristyl lactate, cetyl lactate, isopropyl myristate, myristylmyristate, isopropyl palmitate, isopropyl adipate, isopropy stearate, butyl stearate, decyloleate, cholesterol isostearate, glycerol monostearate, glycerol distearate, glycerol tristearate, alkyl lactate, alkyl citrate and alkyl tartrate, higher alcohols such as lauryl, cetyl, stearyl, oleyl, behenyl, cholesterol and 2-hexydecanol alcohol, essential oils and extracts thereof and mixtures of any of the foregoing components. Diluents may be selected from water or alcohols such as ethanol. Humectants could be chosen from consisting of polyols such as glycerin, sorbitol, propylene glycol, butylene glycol, hexylene glycol, ethoxylated glucose, 1, 2-hexane diol, hexanetriol, dipropylene glycol, erythritol, trehalose, diglycerin, xylitol, maltitol, maltose, glucose, fructose, sodium chondroitin sultate, sodium hyaluronate, sodium adenosin phosphate, sodium lactate, pyrrolidone carbonate, glucosamine, cyclodextrin, and mixtures thereof. Chelants may be chosen from ethylenediaminetetraacetate or ethane 1-hydroxy-1, 1-diphosphonate. Preservatives may be selected from the group consisting of methyl paraben, propyl paraben, phenoxyethanol, methylisothiazolinone, sodiumhydroxymethylglycinate, diazolidinyl urea, benzyl alcohol, caprylyl glycol, ethyl hexyl glycerin, sodium benzoate, potassium benzoate, sorbic acid, potassium sorbate and mixtures thereof.
The levels of structurants in the formulation may range from 0.001% to 5%, chelants from 0.01% to 0.5%, emollients may be used in the range of 0.01% to 30%, diluents to the range of 1% to 90%, humectants may be present from 0.01% to 10%, actives may vary from 0.0001% to 15%, neutralizers, pH modifiers, preservatives and fragrances may be added as per the requirement.
The personal care compositions of the present invention can be prepared by conventional process. Typically such process comprises steps of:
a) heating the water phase ingredients to the desired temperature,
b) heating the oil phase ingredients to the desired temperature (75 °C to 80 °C),
c) mixing the oil and water phase ingredients at a temperature of 75°C to 80°C under constant stirring for 15 to 25 min and said mixing speed,
d) homogenizing the formulation,
e) addition of bioactives, preservatives and fragrances and
f) completion of the batch by stirring at said speed until a homogenous emulsion/ suspension/ gel is obtained.
The examples, which are intended to be purely exemplary of the invention, should therefore not be considered to limit the invention in any way.

Example 1
An anti-aging facial hydrogel formulation in accordance with the present invention can have the composition as mentioned in Table 1 below

Table 1: An anti-aging facial hydrogel comprising naringenin and naringin
Phase Name of ingredient % wt Functionality
A Water 91.90
A Disodium EDTA 0.05 Chelant
A Carbomer 0.50 Structurant
A Glycerine 3.00 Humectant
A Propylene glycol 2.00 Humectant
B Dimethicone 1.00 Feel enhancer
C Triethanol amine 0.75 Neutralizer
D Naringenin 0.10 Active
D Naringin 0.30 Active
E Phenoxyethanol 0.40 Preservative

Process for preparing the anti-aging facial hydrogel
Step 1 : All ingredients in Phase A are weighed and dispersed and stirred for 15 min at 300 rpm.
Step 2 : Ingredients of Phase B are weighed and mixed and added to above mix and stirred for 10 min at 300 rpm.
Step 3 : The above mixture was neutralized using ingredient C and stirred for 10 min at 400 rpm.
Step 4 : Phase D ingredients were weighed and added and stirred for 5 min at 300 rpm.
Step 5 : The batch was completed by adding phase E and stirring for 5 min at 300 rpm.

Example 2
An anti-aging serum formulation in accordance with the present invention can have the composition as mentioned in Table 2 below

Table 2: An anti-aging serum comprising naringenin and naringin
Phase Name of ingredient % wt Functionality
A Water 85.93
A Disodium EDTA 0.05 Chelant
A Carbomer 0.05 Structurant
A Xanthum gum 0.10 Structurant
A Glycerine 3.00 Humectant
A Butylene glycol 2.00 Humectant
B Caprylic/Capric triglyceride 5.00 Emollient
B Isopropyl myristate 2.00 Emollient
B Dimethicone 1.00 Feel enhancer
C Triethanol amine 0.08 Neutralizer
D Naringenin 0.20 Active
D Naringin 0.20 Active
E Phenoxyethanol 0.40 Preservative

Process for preparing the anti-aging serum
Step 1 : All ingredients in Phase A are weighed and dispersed and stirred for 15 min at 300 rpm.
Step 2 : Ingredients of Phase B are weighed and mixed and added to above mix and stirred for 10 min at 300 rpm.
Step 3 : The above mixture was neutralized using ingredient C and stirred for 10 min at 400 rpm.
Step 4 : Phase D ingredients were weighed and added and stirred for 5 min at 300 rpm.
Step 5 : The batch was completed by adding phase E and stirring for 5 min at 300 rpm.

Example 3
Experiments to show the synergistic activity of combination of naringenin and naringin in accordance with the present invention
Sample composition
Step 1: Naringenin was weighed in a beaker and appropriate amount of water (100 ml) was added to make the final concentration as 1%;
Step 2: Naringin was weighed into another beaker and appropriate amount of water (100 ml) was added to make the final concentration as 1%;
Step 3: The contents were allowed to dissolve completely in room temperature or vortexed if needed;
Step 4: All the contents were transferred to a container and stored at 2-8°C.
Different samples were prepared in accordance with the above method by varying the combining ratio of naringenin and naringin as mentioned in Table 3.

Experimental details
Countable number of C. elegans (~20) was dispensed inside individual wells of microtiter plate containing M9 buffer or distilled water. The total reaction volume was 500µl. The individual active compounds were added in such a way that the final concentration of the compound inside the mix will be 0.01%.
In the case of synergistic activity, the total volume of the active compound inside a well was made as 0.01%. In other words, the combined concentration of both the compounds inside the well was 0.01%.
The worms were counted manually through a Steriomicroscope at every 8 hour interval. Worms were considered dead when they did not respond to a gentle tap of the plate or to a touch with a platinum wire pick. Each experimental condition was done in triplicates and the rate of survival was plotted graphically.

Table.3 depicts the activity of combination of naringenin and naringin.
No. of days survived by C elegans
Control Naringenin Naringin Combination ratio (GT: Naringin) Longevity Synergistic activity
21 23 23 1:1 25 Yes
2:1 25 Yes
3:1 27 Yes
4:1 20 No
5:1 18 No
1:2 26 Yes
1:3 28 Yes
1:4 21 No
1:5 19 No

From the Table.3, it is observed that the combination of Naringenin and Naringin synergistically enhances the longevity of C. elegans as compared to Naringenin and Naringin when provided alone. Further the synergism is obtained only in the ratio of 1:3 to 3:1.

Example 4
Experiments to show the synergistic activity of combination of naringenin and naringin with UV exposure in accordance with the present invention
C. elegans and the active compounds were dispensed to the microtitre plate as mentioned in Example 3. Then the plate was exposed to UV at 365 nm constantly for 3 hours.

Table.4 depicts the synergistic activity of Naringenin and Naringin combination with UV exposure.
No. of days survived by C elegans when exposed to UV rays
Control Naringenin Naringin Combination ratio (GT: Naringin) Longevity Synergistic activity
17 19 20 1:1 22 Yes
2:1 22 Yes
3:1 24 Yes
4:1 20 No
5:1 16 No
1:2 23 Yes
1:3 25 Yes
1:4 20 No
1:5 17 No

From Table.4, it is observed that the combination of Naringenin and Naringin synergistically enhances the longevity of C. elegans when exposed to UV light as compared to Naringenin and Naringin when taken alone. Further the synergism is obtained only in the ratio of 1:3 to 3:1.

EXAMPLE 5
The combination of two molecules always do not extend the lifespan or work synergistically, each known to extend the lifespan of C. elegans organisms. This phenomenon is illustrated with the example of Folic acid and Quercetin combination in ratio of 1:1 (Total concentration of actives maintained at 0.01%).
Experiments were performed by the same process as mentioned in example 3 and the results obtained are mentioned in Table 5 below.

Table.5 depicts the effect of combination of Folic acid and Quercetin in ratio of 1:1 on lifespan of C.elegans.
Life-span of C. elegans (in days)
Control Folic acid Quercetin 1:1 Folic acid and Quercetin
20 22 24 < 2 days (around 36 hrs)

From table.5, it is observed that the combination of Folic acid and Quercetin in a ratio of 1:1 drastically shorten the Longevity of C. elegans organisms to less than 2 days (36 hours) compare with the Control, Folic acid and Quercetin alone. In fact the combination of Folic acid and Quercetin are antagonistic to each other.