CLIAMS:1. An anti-aging personal care composition comprising: 0.001 to 5% by weight green tea extract and 0.001 to 3 % by weight Kappaphycus extract, wherein said green tea extract and said Kappaphycus extract are present in a weight ratio from 4:1 to 1:3.
2. The anti-aging personal care composition as claimed in claim 1, wherein said composition comprises 0.01% w/v of said green tea extract and 0.01% w/v of said Kappaphycus extract.
3. The anti-aging personal care composition as claimed in any one of preceding claim, wherein said composition comprises 0.01% w/v of said green tea extract and 0.01% w/v of said Kappaphycus extract; and said green tea extract and said Kappaphycus extract being present in a weight ratio from 4:1 to 2:1.
4. The anti-aging personal care composition as claimed in any one of preceding claim, said composition comprising further cosmetically acceptable excipients selected from a group of structurants, chelants, diluents, humectants, emollients, actives, neutralizers, pH modifiers, preservatives and fragrances.
5. The anti-aging personal care composition as claimed in any one of preceding claim, wherein said composition is prepared in the form of lotions, creams, serums, oils, milks, sprays, gels and gel-creams.
,TagSPECI:FILED OF INVENTION
The present invention relates to personal care compositions. More specifically it relates to anti-aging composition comprising natural extracts.

BACKGROUND OF INVENTION
Skin is subject to deterioration through dermatological disorders, environmental abuse (wind, air conditioning, and central heating) or through the normal aging process (chronoageing) which may be accelerated by exposure of skin to sun (photoageing).
Since thousands of years people have been conscious about need of skin care for various reasons, including cleanliness, comfort, beauty, and prevention of wrinkles, aging and protection against the sun. Research has confirmed the benefits of proper skin care through the use of products that contain anti-aging ingredients, primarily actives, anti-oxidants and sunscreens.
Skin care formulations using various extracts and actives for curing age related skin conditions are known in various prior art. For example, US7820177 exemplifies and claims a self-adhesive polymer matrix which comprises (a) at least one polymer which forms a gel in water, (b) water, (c) a sea algae extract, and (d) at least one alcohol which is a monohydric or polyhydric alcohol and (e) at least one dermatological or cosmetic active substance for skin improvement. Further, the prior art suggest that adding green tea at a proportion from 0.02% to 35% may give some beneficial effect.
US6572868 provides a cosmetic composition which enhances the retexturization of the skin, producing significantly improved smoothness, as well as significantly increasing the firmness and moisture content of the skin and regulating wrinkles of the skin. The restructuring composition comprises safe and effective amounts of carrageenans, borage seed oil, squalane, ceramide 3, ceramide 6, red algae extract, dipalmitoylhydroxyproline and oleuropein. The prior art added green tea as a general antioxidant additive. Further, it does not disclose about ratios on which the extracts of green tea and kappaphycus are need to be combined to get desired anti-aging effect.
There are various products in the market which comprises of natural extracts such as Green Tea Facial Mask from the Tea House Pattaya (http://teahousepattaya.com/index.php?page=shop.product_details&category_id=3&flypage=flypage.tpl&product_id=53&vmcchk=1&option=com_virtuemart&Itemid=2) which contains Green tea Extract Tocopherol, Glycerine, Sodium Lactate, Sucrose, Sodium Citrate, Malic Acid, Tartaric Acid, Kaolin, Euphorbia Cerifera (Candelilla) Wax, Methylisothiazolinone, Kappaphycus alverazii Extract. It is a facial mask for freshening or instant rejuvenation benefits. These are not leave-on products and are not meant for every-day use. Normally, anti-aging benefits cannot be provided/ achieved by such products and neither does the product seek to achieve any anti ageing effect.
There is always need for more efficient easily available actives for skin care compositions that enhance appearance. The demand for cosmetic compositions and cosmetic methods for anti-aging skin products has grown enormously.

OBJECTIVES OF THE PRESENT INVENTION
It is an object of the present invention to overcome the drawbacks of the prior art.
It is another object of the present invention to provide a cosmetic composition containing extracts of green tea, and Kappaphycus extract to provide good anti-aging effect.

SUMMARY OF THE PRESENT INVENTION
According to the present invention there is provided an anti-aging personal care composition comprising 0.001% to 5% by weight green tea extract and 0.001% to 3% by weight Kappaphycus extract, wherein the green tea extract and the Kappaphycus extract are present in a weight ratio from 4:1 to 1:3.

BRIEF DESCRIPTION OF ACCOMPANYING DRAWINGS
Fig. 1 illustrates the representative graph showing the longevity given by both Green tea extract and Kappaphycus extract at 0.01% concentration.
Fig. 2 illustrates the representative graph showing the synergistic activity of Green tea extract and Kappaphycus extract in different ratios.

DETAILED DESCRIPTION OF PRESENT INVENTION
The present invention provides a personal care composition containing extracts of green tea and a red algae, kappaphycus which provides anti-aging effect. The inventors have surprisingly found that when green tea extract and kappaphycus extract are combined at ratios anywhere from 4:1 to 1:3 exhibit a synergistic anti-aging effect.

The present invention provides a holistic approach to address aging rather than conventional approaches wherein multiple pathways contributing to aging are studied, targets identified and molecules working towards particular targets are combined to obtain an anti-aging product.

The present inventors have used Caenorhabditis elegans (C. elegans), which is a simple multicellular organism, as a model system for studying the aging process. It is known that actives/ molecules that improve the life span of Caenorhabditis elegans is used to develop anti-aging products.
The present inventors have found that the combination of green tea extract and Kappaphycus extract at a certain weight ratio synergistically improves the life span of C. elegans.
Thus according to a preferred embodiment of the present invention there is provided an antiaging composition comprising 0.001% to 5% by weight green tea extract and 0.001% to 3% by weight Kappaphycus extract, wherein the green tea extract and the Kappaphycus extract are present in a weight ratio from 4:1 to 1:3.
According to a preferred embodiment of the present invention the antiaging composition comprises 0.01% w/v of green tea extract and 0.01% w/v of Kappaphycus extract in varying ratios from 4:1 to 2:1 or 1:3.
The anti-aging personal care composition as claimed in claim 1, wherein said composition is prepared in the form of lotions, creams, serums, oils, milks, sprays, gels and gel-creams.
The personal care compositions of the present invention may further comprise cosmetically acceptable excipients selected from a list of structurants, chelants, emollients, diluents, humectants, actives, neutralizers, pH modifiers, preservatives and fragrances. The list of structurants employed may include but not be limited to starch, modified starch, guar gum, carbomers, silicone based polymers, acrylates and cellulose based polymers. Emollients that may be employed include silicone oils and modifications thereof such as linear and cyclic polydimethylsiloxanes, volatile silicones, esters such as cetyloctanoate, myristyl lactate, cetyl lactate, isopropyl myristate, myristylmyristate, isopropyl palmitate, isopropyl adipate, isopropy stearate, butyl stearate, decyloleate, cholesterol isostearate, glycerol monostearate, glycerol distearate, glycerol tristearate, alkyl lactate, alkyl citrate and alkyl tartrate, higher alcohols such as lauryl, cetyl, stearyl, oleyl, behenyl, cholesterol and 2-hexydecanol alcohol, essential oils and extracts thereof and mixtures of any of the foregoing components. Diluents may be selected from water or alcohols such as ethanol. Humectants could be chosen from consisting of polyols such as glycerin, sorbitol, propylene glycol, butylene glycol, hexylene glycol, ethoxylated glucose, 1, 2-hexane diol, hexanetriol, dipropylene glycol, erythritol, trehalose, diglycerin, xylitol, maltitol, maltose, glucose, fructose, sodium chondroitin sultate, sodium hyaluronate, sodium adenosin phosphate, sodium lactate, pyrrolidone carbonate, glucosamine, cyclodextrin, and mixtures thereof. Chelants may be chosen from ethylenediaminetetraacetate or ethane 1-hydroxy-1, 1-diphosphonate. Preservatives may be selected from the group consisting of methyl paraben, propyl paraben, phenoxyethanol, methylisothiazolinone, sodiumhydroxymethylglycinate, diazolidinyl urea, benzyl alcohol, caprylyl glycol, ethyl hexyl glycerin, sodium benzoate, potassium benzoate, sorbic acid, potassium sorbate and mixtures thereof.
The levels of structurants in the formulation may range from 0.001% to 5%, chelants from 0.01% to 0.5%, emollients may be used in the range of 0.01% to 30%, diluents to the tune of 1% to 90%, humectants may be present from 0.01% to 10%, actives may vary from 0.0001% to 15%, neutralizers, pH modifiers, preservatives and fragrances may be added as per the requirement.
The personal care compositions of the present invention can be prepared by conventional process. Typically such process comprises steps of : a) heating the water phase ingredients to the desired temperature, b) heating the oil phase ingredients to the desired temperature (75°C to 80°C), c) mixing the oil and water phase ingredients at a temperature of 75°C to 80°C under constant stirring for 15 to 25 min and said mixing speed, d) homogenizing the formulation, e) addition of bioactives, preservatives and fragrances and f) completion of the batch by stirring at said speed until a homogenous emulsion/ suspension/ gel is obtained.

The examples, which are intended to be purely exemplary of the invention, should therefore not be considered to limit the invention in any way.

Example 1
An anti-aging night cream formulation in accordance with the present invention can have the composition as mentioned in Table 1 below
Table 1: An anti-aging night cream with green tea and Kappaphycus extracts.
S. No. Phase INCI Weight %
1 A Water 82.02
2 Disodium EDTA 0.05
3 Glycerine 2.86
4 Propylene Glycol 2.00
5 Carbomer 0.25
6 B STEARETH-21 1.20
7 Steareth-2 0.60
8 Isopropyl myristate 1.00
9 Isohexadecane 1.00
10 Glyceryl Stearate 0.50
11 Mineral Oil 4.00
12 Cetyl alcohol 1.00
13 Glycol Distearate 1.00
14 Methyl Paraben 0.16
15 Propyl Paraben 0.04
16 C Triethanolamine 0.35
17 D Cyclopentasiloxane (and) Phenyl Trimethicone (and) Dimethiconol (and) C12-15 Alkyl Benzoate (and) DimethiconeCrosspolymer 1.42
18 E Kappaphycus extract 0.50
19 Green tea extract 0.50
20 F Phenoxetol 0.20
21 G Fragrance 0.35

The above anti-aging night cream was prepared by the following process.
Step 1: Phase A ingredients are weighed in a beaker and heated to 80°C for 15 minutes;
Step 2: Phase B ingredients are weighed in a beaker and heat to 80°C for 15 minutes;
Step 3: Phase B ingredients of step 2 are added to phase A ingredients of step 1 and the mixture is homogenized at 5000 RPM for 15-20 minutes;
Step 4: Phase C ingredients are mixed with 1% water and added to the mixture of step 3 and the resultant mixture is stirred for 5 minutes at 500 RPM;
Step 5: Phase D ingredients are added to the mixture of step 4 and the resultant mixture is stirred for 5 min at 500 RPM;
Step 6: Phase E ingredients are added to the mixture of step 5 and mixed for 5 min at 300 RPM;
Step 7: Phase F ingredients are added to the mixture of step 6 and mixed for 5 min at 300 RPM;
Step 8: Phase G ingredients are added to the mixture of step 7 and mixed for 5 min at 300 rpm to prepare the final product.

Example 2
An anti-aging facial hydrogel formulation in accordance with the present invention can have the composition as mentioned in Table 2 below

Table 2: An anti-aging facial hydrogel with green tea and Kappaphycus extract.
Name of ingredient % wt Functionality
Water 85.13 Diluent
Disodium EDTA 0.05 Chelant
Carbomer 0.05 Structurant
Xanthum gum 0.10 Structurant
Glycerine 3.00 Humectant
Butylene glycol 2.00 Humectant
Green tea extract 0.90 Active
Kappaphycus 0.30 Active
Caprylic/Capric triglyceride 5.00 Emollient
Isopropyl myristate 2.00 Emollient
Triethanol amine 0.08 Neutralizer
Dimethicone 1.00 Emollient
Phenoxyethanol 0.40 Preservative

The above anti-aging facial hydrogel formulation was prepared by the following process.
Step 1: Carbomer was dispersed in water and stirred for 7 min at 300 rpm;
Step 2: To the above, chelant, structurant, humectants were added and stirred for 5 min at 500 rpm until the mixture is homogenous;
Step 3: The emollients are added and stirred for 5 min at 300 rpm;
Step 4: A dilute solution of the actives was prepared in water and added to the mixture obtained in step 3 under constant stirring at 400 rpm over 5 min;
Step 5: The formulation obtained in step 4 was neutralized with TEA so as to attain the desired pH and viscosity under constant stirring at 300 rpm.
Step 6: Adding preservatives and mix at 500 rpm for 5 min until a homogenous hydrogel is obtained.

Example 3
Experiments to show the synergistic activity of combination of green tea extract and Kappaphycus extract in accordance with the present invention
Sample composition
Step 1: Green tea extract was weighed in a beaker and appropriate amount of water (100 ml) was added to make the final concentration as 1%;
Step 2: Kappaphycus was weighed into another beaker and appropriate amount of water (100 ml) was added to make the final concentration as 1%;
Step 3: The contents were allowed to dissolve completely in room temperature or vortexed if needed;
Step 4: All the contents were transferred to a container and stored at 2-8°C.
Different samples were prepared in accordance with the above method by varying the combining ratio of Green tea and Kappaphycus as mentioned in Table 3.

Experimental details
Countable number of C. elegans (~20) was dispensed inside individual wells of microtiter plate containing M9 buffer or distilled water. The total reaction volume was 500µl. The individual active compounds were added in such a way that the final concentration of the compound inside the mix will be 0.01%.
In the case of synergistic activity, the total volume of the active compound inside a well was made as 0.01%. In other words, the combined concentration of both the compounds inside the well was 0.01%.
The worms were counted manually through a Steriomicroscope at every 8 hour interval. Worms were considered dead when they did not respond to a gentle tap of the plate or to a touch with a platinum wire pick. Each experimental condition was done in triplicates and the rate of survival was plotted graphically. Fig. 1 shows the longevity given by both Green tea extract and Kappaphycus extract at 0.01% concentration. Fig. 2 shows the synergistic activity of Green tea extract and Kappaphycus extract in different ratios. The total percentage of the actives inside the reaction was maintained at 0.01%.
Table.3 depicts the synergistic activity of combination of green tea extract and Kappaphycus extract.
No. of days survived by C. elegans
Control Green tea Kappaphycus Combination ratio (Green tea: Kappaphycus) Longevity Synergistic activity
21 24 23 1:1 26 Yes
2:1 28 Yes
3:1 30 Yes
4:1 26 Yes
1:2 27 Yes
1:3 29 Yes
From the Table.3, it is observed that the combination of green tea and Kappaphycus extracts are synergistically enhances the longevity of C. elegans compare with the green tea and Kappaphycus alone.

Example 4
Experiments to show the synergistic activity of combination of green tea extract and Kappaphycus extract with UV exposure in accordance with the present invention
C. elegans and the active compounds were dispensed to the microtitre plate as mentioned in Example 3. Then the plate was exposed to UV at 365 nm constantly for 3 hours.
Table.4 depicts the synergistic activity of green tea and kappaphycus extract combination with UV exposure.
No. of days survived by C. elegans(with UV exposure)
Control Green tea Kappaphycus Combination ratio (Green tea: Kappaphycus) Longevity Synergistic activity
17 21 18 1:1 23 Yes
2:1 24 Yes
3:1 27 Yes
4:1 23 Yes
1:2 23 Yes
1:3 25 Yes
1:4 22 Yes

From the Table.4, it is observed that the combination of green tea and Kappaphycus extracts synergistically enhance the longevity of C. elegans in presence of UV light as compared to green tea and Kappaphycus extracts when used alone.

Example 5
Comparative experiments where green tea extract and the Kappaphycus extract are mixed in weight ratio beyond 4:1 to 1:3.
Experiments were performed with varying combination of green tea extract and the Kappaphycus extract in accordance with the method described in examples 3 and 4. The results obtained as mentioned in Tables 5 and 6 below.

Table 5
No. of days survived by C. elegans
Control Green tea Kappaphycus Combination ratio (Green tea: Kappaphycus) Longevity Synergistic activity
21 24 23 5:1 22 No
1:4 24 No
1:5 21 No

Table 6
No. of days survived by C. elegans (with UV exposure)
Control Green tea Kappaphycus Combination ratio (Green tea: Kappaphycus) Longevity Synergistic activity
17 21 18 5:1 20 No
1:5 18 No

EXAMPLE 6

The combination of any two molecules always do not extend the lifespan or work synergistically, though each may be known to extend the lifespan of C. elegans organisms. This phenomenon was illustrated with the example of Folic acid and Quercetin combination in ratio of 1:1 (Total concentration of actives maintained at 0.01%).
Experiments were performed by the same process as mentioned in example 3 and the results obtained are mentioned in Table 7 below.

Table.7 depicts the effect of combination of Folic acid and Quercetin in ratio of 1:1 on lifespan of C. elegans.
Life-span of C. elegans(in days)
Control Folic acid Quercetin 1:1 Folic acid and Quercetin
20 22 24 < 2 days (around 36 hrs)

From table.7, it is observed that the combination of Folic acid and Quercetin even in a ratio of 1:1 drastically shorten the longevity of C. elegans organisms to less than 2 days (36 hours) compare with the control, Folic acid and Quercetin alone. In fact the combination of Folic acid and Quercetin are antagonistic to each other unlike the specific combination of green tea extract and Kappaphycus extract as in the present invention.