CLIAMS:
1. An anti-aging personal care composition comprising: 0.001% to 2.0% by weight Green tea and 0.001% to 3.0% by weight naringin, wherein said Green tea extract and naringin are present in a weight ratio from 1:3 to 4:1.

2. The anti-aging personal care composition as claimed in claim 1, said composition comprising further cosmetically acceptable excipients selected from structurants, emollients, emulsifiers, diluents, humectants, actives, preservatives and fragrances.

3. The anti-aging personal care composition as claimed in claim 1, wherein said composition is prepared in the form of lotions, serums, creams, oils, sprays, gels and gel-creams .

,TagSPECI:FILED OF INVENTION
The present invention relates to personal care compositions. More specifically it relates to anti-aging composition comprising natural products.

BACKGROUND OF INVENTION
Skin is subjected to deterioration through dermatological disorders, environmental abuse (wind, air conditioning, and central heating) or through the normal aging process (chronoageing) which may be accelerated by exposure of skin to sun (photoageing).
Since thousands of years people have been conscious about need of skin care for various reasons, including cleanliness, comfort, beauty, and prevention of wrinkles, aging and protection against the sun. Research has confirmed the benefits of proper skin care through the use of products that contain anti-aging ingredients, primarily actives, anti-oxidants and sunscreens.
Skin care formulations using various extracts and actives for curing skin conditions are known in various prior art. For example, US2010/0166897 discloses a composition, especially a cosmetic or dermatological composition containing, in a physiologically acceptable medium, a combination of at least one monosaccharide chosen from mannose, rhamnose and a mixture thereof, and of at least one antioxidant.The antioxidant is capable of increasing the number of keratinocytes and the synthesis of type 1 procollagen thus controlling the signs of ageing of the skin. Polyphenols are also one type of antioxidant used in US‘897. The polyphenol compounds have been derived from various plant extracts including green tea extracts. Most popular anti-aging actives in prior art include peptides and small molecules for collagen boost. They help in the maintenance of the integrity of the extra-cellular matrix (ECM). Also equally popular are enzyme inhibitors that target matrix-metallo proteases (MMPs) and anti-oxidants that protect the cellular components from the assault of free radicals. Some patents also talk of polysaccharide moieties and herbal compounds.
One of the drawbacks of usage of high levels of herbal components in cosmetic formulations is that these extracts at high concentrations in cosmetic formulations causes color change in the formula, gives rise to redox issues and affects the overall stability and aesthetics of the formulation.
For instance, JP2000319154 discloses use of flavonoids, coumarins, phenylpropanoids and tannins to prevent photo-toxicity. The cosmetic composition disclosed contains the photo-toxicity inhibitor which has a photo-toxicity inhibiting action against air pollutants and is useful for depressing various adverse effects on the human body, such as skin cancer, skin roughness, skin aging, sunburn (melanization) and inflammation.
Addressing anti-aging has also been attempted through combining many ingredients targeting several pathways. As on date, there has been no single method to address aging holistically.
At the same time, there is always need for more efficient, easily available actives for skin care compositions that enhance appearance and provide anti-ageing effect. The demand for cosmetic compositions and cosmetic methods for anti-aging skin products has grown enormously. Accordingly the present invention provides anti-aging products with combination of green tea extract and a molecule naringin in a specific ratio where the actives are present in low amount.

OBJECTIVES OF THE PRESENT INVENTION
It is an object of the present invention to overcome the drawbacks of the prior art.
It is another object of the present invention to provide a cosmetic composition comprising Green tea extract and Naringin.

SUMMARY OF THE PRESENT INVENTION
According to the present invention there is provided an anti-aging personal care composition comprising: 0.001% to 2.0% by weight Green tea and 0.001% to 3.0% by weight Naringin, wherein said Green tea and Naringin are present in a weight ratio from 1:3 to 4:1.

DETAILED DESCRIPTION OF PRESENT INVENTION
The present invention provides a personal care composition containing green tea extract and a flavonoid, Naringin which improves anti-aging. The inventors have surprisingly found that when green tea extract and naringin are combined at ratios anywhere from 1:3 to 4:1 exhibit a synergistic effect.
The present invention provides a holistic approach to address aging rather than conventional approaches wherein multiple pathways contributing to aging are studied, targets identified and molecules working towards particular targets are combined to obtain an anti-aging product.
The present inventors have used Caenorhabditis elegans (C. elegans), which is a simple multicellular organism, as a model system for studying the aging process. It is known that actives/ molecules that improve the life span of C. elegans is used to develop anti-aging products.
The present inventors have found that the combination of green tea extract and naringin at a certain weight ratio synergistically improves the life span of C. elegans.
The present invention thus provides an anti-aging composition comprising 0.001% to 2.0% by weight green tea extract and 0.001% to 3.0% by weight naringin, wherein said green tea extract: said naringin is in a weight ratio from 1:3 to 4:1.
The personal care compositions of the present invention may further comprise cosmetically acceptable excipients selected from list of structurants, emollients, emulsifiers, diluents, humectants, actives, preservatives and fragrances. The list of structurants employed may include but not be limited to starch, modified starch, guar gum, carbomers, silicone based polymers, acrylates and cellulose based polymers. Emollients that may be employed include silicone oils and modifications thereof such as linear and cyclic polydimethylsiloxanes, volatile silicones, esters such as cetyloctanoate, myristyl lactate, cetyl lactate, isopropyl myristate, myristyl myristate, isopropyl palmitate, isopropyl adipate, isopropyl stearate, butyl stearate, decyloleate, cholesterol isostearate, glycerol monostearate, glycerol distearate, glycerol tristearate, alkyl lactate, alkyl citrate and alkyl tartrate, higher alcohols such as lauryl, cetyl, stearyl, oleyl, behenyl, cholesterol and 2-hexydecanol alcohol, essential oils and extracts thereof and mixtures of any of the foregoing components. Diluents may be selected from water or alcohols such as ethanol. Humectants could be chosen from consisting of polyols such as glycerin, sorbitol, propylene glycol, butylene glycol, hexylene glycol, ethoxylated glucose, 1, 2-hexane diol, hexanetriol, dipropylene glycol, erythritol, trehalose, diglycerin, xylitol, maltitol, maltose, glucose, fructose, sodium chondroitin sultate, sodium hyaluronate, sodium adenosin phosphate, sodium lactate, pyrrolidone carbonate, glucosamine, cyclodextrin, and mixtures thereof. Chelants may be chosen from ethylenediaminetetraacetate or ethane-1-hydroxy-1, 1-diphosphonate. Preservatives may be selected from the group consisting of methyl paraben, propyl paraben, phenoxyethanol, methylisothiazolinone, sodium hydroxymethylglycinate, diazolidinyl urea, benzyl alcohol, caprylyl glycol, ethyl hexyl glycerin, sodium benzoate, potassium benzoate, sorbic acid, potassium sorbate and mixtures thereof. The levels of structurants in the formulation may range from 0.001% to 5%, chelants from 0.01% to 0.5%, emollients may be used in the range of 0.01% to 30%, emulsifiers may be used in the range of 0.01% to 8%, diluents to the tune of 1% to 90%, humectants may be present from 0.01% to 10%, actives may vary from 0.0001% to 15%, neutralizers, pH modifiers, preservatives and fragrances may be added as per the requirement.
The personal care compositions of the present invention can be prepared by conventional process. Typically such process comprises steps of: a) Heating up the water phase ingredients to the desired temperature, b) Heat up the oil phase ingredients to the desired temperature, c) Mixing the oil and water phase ingredients at a said temperature under constant stirring for a said time and said mixing speed, d) Homogenizing the formulation under said conditions, e) Addition of bioactives, preservatives and fragrances and f) Completion of the batch by stirring at said speed until a homogenous emulsion/ suspension/ gel is obtained.
The examples, which are intended to be purely exemplary of the invention, should therefore not be considered to limit the invention in any way.

Example 1
An anti-aging cream formulation in accordance with the present invention can have the composition as mentioned in Table 1 below

Table 1: An anti-aging cream comprising green tea and naringin
S.No Phase INCI Weight %
1 A Water 86.93
2 Disodium EDTA 0.10
3 Glycerine 2.00
4 Propylene Glycol 2.00
5 Butylene Glycol 1.00
6 D-Panthenol 0.20
7 Acrylates/C 10-30 Alkyl Acrylate Crosspolymer 0.25
8 B Steareth-21
1.20
9 Steareth-2 0.60
10 Isopropyl palmitate 2.00
11 Glyceryl Stearate 0.50
12 Methyl Paraben 0.16
13 Propyl Paraben 0.04
14 C Triethanolamine 0.35
15 D Cyclopentasiloxane (and) Phenyl Trimethicone (and) Dimethiconol (and) C12-15 Alkyl Benzoate (and) DimethiconeCrosspolymer 1.42
16 E Green Tea extract 0.50
17 Naringin 0.20
18 F Phenoxyethanol 0.20
19 G Fragrance 0.35

Process:
Step 1: Phase A ingredients are weighed in a beaker and heated to 80°C for 15 minutes.
Step 2: Phase B ingredients are weighed in a beaker and heated to 80°C for 15 minutes.
Step 3: Phase B ingredients are added to phase A ingredients and the mixture was homogenized at 5000 RPM for 15-20 minutes.
Step 4: Phase C ingredients was mixed with 1% water and added to step 3 mixture and stirred for 5 min at 500 RPM.
Step 5: Phase D ingredients were added to step 4 mixture and stirred for 5 min at 500 RPM
Step 6: Phase E ingredients were added to the above mixture of step 5 and mixed for 5 min at 300 RPM.
Step 7: Phase F ingredients were added to the above mixture of step 6 and mixed for 5 min at 300 RPM.
Step 8: Phase G ingredients were added to step 7 mixture and mixed for 5 min at 300 rpm to complete the batch.

Example 2
An anti-aging serum formulation in accordance with the present invention can have the composition as mentioned in Table 2 below.
Table 2: An anti-aging serum comprising Green tea extract and naringin
Phase Name of ingredient % wt
A Water 85.53
A Disodium EDTA 0.05
A Carbomer 0.05
A Xanthum gum 0.10
A Glycerine 3.00
A Butylene glycol 2.00
B Caprylic/Capric triglyceride 5.00
B Isopropyl myristate 2.00
B Dimethicone 1.00
C Triethanol amine 0.08
D Green Tea Extract 0.60
D Naringin 0.20
E Phenoxyethanol 0.40

Method of preparation:

Step 1: All ingredients of Phase A are weighed and dispersed and stirred for 15 min at 300 rpm.

Step 2: Ingredients of Phase B are weighed and mixed and added to above mixture and stirred for 10 min at 300 rpm.

Step 3: The above mixture was neutralized using ingredient in C and stirred for 10 min at 400 rpm.
Step 4: Phase D ingredients are weighed and added and stirred for 5 min at 300 rpm.

Step 5: The batch was completed by adding phase E and stirring for 5 min at 300 rpm.

Example 3

Experiments to show the synergistic activity of combination of Green Tea extract and naringin in accordance with the present invention

To evaluate the effect of green tea extract and naringin on longevity of C. elegans, the organisms were treated with 0.01% by weight green tea extract alone and 0.01% by weight naringin alone along with one control set (without any treatment). C. elegans were also treated with various combination ratio of green tea extract and naringin as provided in Table 3. The total concentration of 0.01% is maintained whether green tea extract and naringin are provided alone or in combination. The experimental details are given below:

Sample composition

Step 1: Green Tea extract (Naturex) was weighed in a beaker and appropriate amount of water (100 ml) was added to make the final concentration as 1%;

Step 2: Naringin (Sigma) was weighed into another beaker and appropriate amount of water (100 ml) was added to make the final concentration as 1%;

Step 3: The contents were allowed to dissolve completely in room temperature or vortexed if needed;

Step 4: All the contents were transferred to a container and stored at 2-8°C.

Different samples were prepared in accordance with the above method by varying the combining ratio of Green Tea extract and naringin as mentioned in Table 3.

Longevity assessment

Countable number of C. elegans (~20) was dispensed inside individual wells of microtiter plate containing M9 buffer or distilled water. The total reaction volume was 500µl. The individual active compounds were added in such a way that the final concentration of the compound inside the mix will be 0.01%.
In the case of synergistic activity, the total volume of the active compound inside a well was made as 0.01%. In other words, the combined concentration of both the compounds inside the well was 0.01%.
The worms were counted manually through a Steriomicroscope at every 8 hour interval. Worms were considered dead when they did not respond to a gentle tap of the plate or to a touch with a platinum wire pick. Each experimental condition was done in triplicates and the rate of survival was plotted graphically.

Table.3 depicts the synergistic activity of combination of green tea and naringin.

No. of days survived by C. elegans
Control Green tea Naringin Combination ratio (Green tea: Naringin) Longevity Synergistic activity
21 24 23 1:1 25 Yes
2:1 27 Yes
3:1 29 Yes
4:1 25 Yes
5:1 21 No
1:2 26 Yes
1:3 28 Yes
1:4 22 No
1:5 19 No

From Table.3, it is observed that C. elegans survive for upto 21 days on an average. The lifespan increases by 3 – 4 days when treated with either Green Tea extract or naringin. The combination of Green tea extract and Naringin at specific ratio from 4:1 to 1:3 synergistically enhances the longevity of C. elegans as compared to Green tea extract and Naringin when taken alone.

Example 4

Table.4 depicts the synergistic activity of Green tea extract and Naringin combination with UV exposure.
C. elegans and the active compounds were dispensed to the microtitre plate as mentioned in Example 3. Then the plate was exposed to UV at 365 nm constantly for 3 hours.

No. of days survived by C. elegans when exposed to UV rays
Control
Green ea Naringin Combination ratio (Green tea: Naringin) Longevity Synergistic activity
17 21 20 1:1 22 Yes
2:1 26 Yes
3:1 27 Yes
4:1 24 Yes
5:1 20 No
1:2 24 Yes
1:3 26 Yes
1:4 23 Yes
1:5 18 No

From Table. 4, it is observed that the organisms survive for just 17 days when exposed to UV light. Addition of either Green Tea extract or Naringin increases the lifespan by 3-4 days. However, the combination of Green tea extract and Naringin at specific ratio from 4:1 to 1:4 synergistically enhances the longevity of C. elegans in presence of UV light (for even upto 9 days longer) as compared to treatment with Green tea extract and Naringin taken alone.

Example 5
The combination of two molecules always do not extend the lifespan or work synergistically, although each of them might be known to extend the lifespan of C. elegans organisms. This phenomenon is illustrated with the example of Folic acid and Quercetin combination in ratio of 1:1 (Total concentration of actives maintained at 0.01%).
Experimental details were the same as adopted in example 3. The active molecules used were dilute solutions (1% aqueous) of folic acid and quercetin.

Table.5 depicts the effect of combination of Folic acid and Quercetin in ratio of 1:1 on lifespan of C.elegans.

Life-span of C. elegans (in days)
Control Folic acid Quercetin 1:1 Folic acid and Quercetin
20 22 24 < 2 days (around 36 hrs)

From Table 5, it is observed that the combination of Folic acid and Quercetin in a ratio of 1:1 drastically shorten the longevity of C. elegans to less than 2 days (36 hours) as compared to Control, Folic acid and Quercetin when treated individually -. In fact the combination of Folic acid and Quercetin are antagonistic to each other.