CLIAMS:1. An anti-aging personal care composition comprising: 0.001% to 2.0 % by weight Kappaphycus extract and 0.001% to 3.0 % by weight naringenin, wherein said Kappaphycus extract and naringenin are present in a weight ratio from 1: 3 to 4:1.
2. The anti-aging personal care composition as claimed in claim 1, said composition comprising further cosmetically acceptable excipients selected from structurants, emollients, diluents, emulsifiers, surfactants, humectants, actives, preservatives and fragrances.
3. The anti-aging personal care composition as claimed in claim 1, wherein said composition is prepared in the form of lotions, serums, creams, milks, emulsions, suspensions, oils, sprays, gels and gel-creams.
,TagSPECI:FILED OF INVENTION
The present invention relates to personal care compositions. More specifically it relates to anti-aging composition comprising natural products.

BACKGROUND OF INVENTION
Skin is subject to deterioration through dermatological disorders, environmental abuse (wind, air conditioning, and central heating) or through the normal aging process (chronoageing) which may be accelerated by exposure of skin to sun (photoageing).
Since thousands of years people have been conscious about need of skin care for various reasons, including cleanliness, comfort, beauty, and prevention of wrinkles, aging and protection against the sun. Research has confirmed the benefits of proper skin care through the use of products that contain anti-aging ingredients, primarily actives, anti-oxidants and sunscreens.
Skin care formulations using various extracts and actives for curing skin conditions are known in various prior art. For example, KR20120058737 discloses a cosmetic composition containing red algae to regenerate epidermis and dermis on the skin and to improve anti-wrinkling with high safety. The cosmetic composition for anti-aging contains 0.0001-25 wt% of red algae extract as an active ingredient. The red algae include Gelidium amansii, Gracilaria, gigartina, laver, Acanthopeltis japonica, gracilaria, Ceramium kondoi graphite or Grateloupia Filicina. The cosmetic composition is manufactured in the form of an emulsion, suspension, microemulsion, microcapsule, microgranule, or vesicle dispersion agent of ion type (liposome) and non-ion type.
However the prior art compositions comprising red algal extract are used to fight aging by inducing collagen boost.
An article titled “Antioxidant potential of solvent extracts of Kappaphycus alvarezii (Doty) Doty-An edible seaweed” by K. Suresh Kumar et al., discloses various solvent extracts of Kappaphycus alvarezii, an edible red seaweed (family Solieriaceae) were screened for total phenol content and antioxidant activity using 1, 1-diphenyl-2-picrylhydrazyl (DPPH), ferrous ion chelating activity, reducing power and antioxidant activity assays in a linoleic acid system with ferrothiocyanate reagent (FTC). But, in the linoleic acid system, the ethanol extract proved superior to the synthetic antioxidants butylated hydroxytoluene (BHT).
Further WO 2007136428 discloses a composition comprising homogenate of red microalgae cells, the composition may further comprise a flavonoid which can be selected from the group consisting of hesperidin, naringin, naringenin, hesperitin, nobiletin and tangeretin. Further WO’428 also provided a method of reducing reactive oxygen species (ROS) in a mammal. The method is used to prevent or treat a disease or unwanted conditions associated with ROS or oxidative stress. Further WO’428 provides a cosmeceutical composition may contain one or more microalgal polysaccharides, or a microalgal cell homogenate, and a topical carrier. In some embodiments, the carrier may be any carrier suitable for topical application, such as, but not limited to, use on human skin or human mucosal tissue. However the prior art teaches about methods of utilizing polysaccharides from microalgal extracts. Further the prior art does not teaches about synergistic combination of Kappaphycus extract with Naringenin.
US7820177 relates to a cosmetic or dermatological formulation which is suitable in particular for supplying active cosmetic or dermatological substances to the skin in a particularly effective way and, moreover, for doing so in a form which is particularly cosmetic and pleasant for the user. Further US‘177 discloses a self-adhesive polymer matrix which comprises (a) at least one polymer which forms a gel in water, (b) water, (c) a sea algae extract, and (d) at least one alcohol which is a monohydric or polyhydric alcohol and (e) at least one dermatological or cosmetic active substance for skin improvement. The sea algae extract may comprise agar-agar and /or carrageenan. Further the polymer matrix of US‘177 may comprise at least one active substance selected from creatine, creatinine, alpha-glucosylrutin, taurine, serinol, isoserinol, silymarin, silyphos, lipoic acid, liponamide, green tea extract, vitamin C, 8-hexadecene-1,16-dicarboxylic acid, isoflavone, isoflavonoid-containing plant extracts such as soya and clover extracts, ubiquinone Q10, sericosides, tyrosine sulfate, jojoba oil and aloe vera more preferably, naringin (aurantiin, naringenin 7-rhamnoglucoside). However the prior art does not disclose any property of composition which may be obtained by combining Kappaphycus extract with any of the listed flavones. The prior art teaches about gel formation wherein sea algae extract is one of the components. Further the prior art teaches that addition of anti-oxidants at proportions between 0 to 35% would give some benefit. However, the prior art does not discloses a way in which high efficacy can be obtained by using kappaphycus in lower amounts, especially in a particular ratio at which naringenin needs to be combined with Kappaphycus so that maximum anti-aging activity is achieved.
There is always need for more efficient easily available actives for skin care compositions that enhance appearance and provide anti ageing effect. The demand for cosmetic compositions and cosmetic methods for anti-aging skin products has grown enormously. Accordingly the present invention provides anti-aging products with combination of aqueous extract of Kappaphycus, a red algae and a molecule naringenin in a specific ratio where the actives are present in low amount.

OBJECTIVES OF THE PRESENT INVENTION
It is an object of the present invention to overcome the drawbacks of the prior art.
It is another object of the present invention to provide an anti-aging personal care composition.
It is a further object of the present invention to provide cosmetic composition comprising aqueous extract of Kappaphycus, a red algae and Naringenin.

SUMMARY OF THE PRESENT INVENTION
According to the present invention there is provided an anti-aging personal care composition comprising: 0.001% to 2.0 % by weight Kappaphycus extract and 0.001 to 3.0 % by weight Naringenin wherein said Kappaphycus extract and said Naringenin are present in a weight ratio from 1:3 to 4:1

DETAILED DESCRIPTION OF PRESENT INVENTION
The present invention provides a personal care composition comprising aqueous extract of kappaphycus, a red algae and naringenin molecule which possesses anti-aging property. The present inventors have surprisingly found that when aqueous extract of kappaphycus and naringenin are combined at ratios from 1:3 to 4:1, it provides a synergistic anti-aging effect.
The present invention provides a holistic approach to address aging rather than conventional approaches wherein multiple pathways contributing to aging are studied, targets identified and molecules working towards particular targets are combined to obtain an anti-aging product.
The present inventors have used Caenorhabditis elegans (C. elegans), which is a simple multicellular organism, as a model system for studying the aging process. It is known that actives/ molecules that improve the life span of Caenorhabditis elegans is used to develop anti-aging products.
The present inventors have found that the combination of aqueous extract of kappaphycus and naringenin at a certain weight ratio synergistically improves the life span of Caenorhabditis elegans.
The present invention thus provides an anti aging composition comprising 0.001% to 2.0 % by weight aqueous extract of kappaphycus and 0.001% to 3 % by weight naringenin, wherein said aqueous extract of kappaphycus: said naringenin is in a weight ratio from 1:3 to 4:1.
The personal care compositions of the present invention may further comprise cosmetically acceptable excipients selected from structurants, emollients, diluents, humectants, actives, preservatives and fragrances. The list of structurants employed may include but not be limited to starch, modified starch, guar gum, carbomers, silicone based polymers, acrylates and cellulose based polymers. Emollients that may be employed include silicone oils and modifications thereof such as linear and cyclic polydimethylsiloxanes, volatile silicones, esters such as cetyloctanoate, myristyl lactate, cetyl lactate, isopropyl myristate, myristylmyristate, isopropyl palmitate, isopropyl adipate, isopropy stearate, butyl stearate, decyloleate, cholesterol isostearate, glycerol monostearate, glycerol distearate, glycerol tristearate, alkyl lactate, alkyl citrate and alkyl tartrate, higher alcohols such as lauryl, cetyl, stearyl, oleyl, behenyl, cholesterol and 2-hexydecanol alcohol, essential oils and extracts thereof and mixtures of any of the foregoing components. Diluents may be selected from water or alcohols such as ethanol. Humectants could be chosen from consisting of polyols such as glycerin, sorbitol, propylene glycol, butylene glycol, hexylene glycol, ethoxylated glucose, 1, 2-hexane diol, hexanetriol, dipropylene glycol, erythritol, trehalose, diglycerin, xylitol, maltitol, maltose, glucose, fructose, sodium chondroitin sultate, sodium hyaluronate, sodium adenosin phosphate, sodium lactate, pyrrolidone carbonate, glucosamine, cyclodextrin, and mixtures thereof. Chelants may be chosen fromethylenediaminetetraacetate or ethane 1-hydroxy-1, 1-diphosphonate. Preservatives may beselected from the group consisting of methyl paraben, propyl paraben, phenoxyethanol, methylisothiazolinone, sodiumhydroxymethylglycinate, diazolidinyl urea, benzyl alcohol, caprylyl glycol, ethyl hexyl glycerin, sodium benzoate, potassium benzoate, sorbic acid, potassium sorbate and mixtures thereof. The levels of structurants in the formulation may range from 0.001% to 5%, chelants from 0.01% to 0.5%, emollients may be used in the range of 0.01% to 30%, diluents to the range of 1% to 90%, humectants may be present from 0.01% to 10%, actives may vary from 0.0001% to 15%, neutralizers, pH modifiers, preservatives and fragrances may be added as per the requirement.
The personal care compositions of the present invention may be in the form of a gel, cream, lotion, spray, milk, emulsion, suspension, hydrogel, oil and serum.
The personal care compositions of the present invention can be prepared by conventional process. Typically such process comprises steps of:
a) heating up the water phase ingredients to the desired temperature,
b) heating up the oil phase ingredients to the desired temperature,
c) mixing the oil and water phase ingredients at a said temperature under constant stirring for a said time and said mixing speed,
d) homogenizing the formulation under said conditions,
e) addition of bioactives, preservatives and fragrances and
f) completion of the batch by stirring at said speed until a homogenous emulsion/ suspension/ gel is obtained.
The examples, which are intended to be purely exemplary of the invention, should therefore not be considered to limit the invention in any way.

Example 1
An anti-aging cream formulation in accordance with the present invention can have the composition as mentioned in Table 1 below
Table 1: An anti-aging cream comprising aqueous extract of Kappaphycus and naringenin
S.No Phase INCI Weight %
1 A Water 81.62
2 Disodium EDTA 0.05
3 Glycerine 2.86
4 Propylene Glycol 2.00
5 Carbomer 0.25
6 B STEARETH-21 1.20
7 Steareth-2 0.60
8 Isopropyl myristate 1.00
9 Isohexadecane 1.00
10 Glyceryl Stearate 0.50
11 Mineral Oil 4.00
12 Cetyl alcohol 1.00
13 Glycol Distearate 1.00
14 Methyl Paraben 0.16
15 Propyl Paraben 0.04
16 C Triethanolamine 0.35
17 D Cyclopentasiloxane (and) Phenyl Trimethicone (and) Dimethiconol (and) C12-15 Alkyl Benzoate (and) Dimethicone Crosspolymer 1.42
18 E Kappaphycus extract 0.20
19 Naringenin 0.20
20 F Phenoxetol 0.20
21 G Fragrance 0.35

The above cream was prepared by the process as mentioned below
Step 1: Phase A ingredients are weighed in a beaker and heated to 80°C for 15 minutes.
Step 2: Phase B ingredients are weighed in a beaker and heated to 80°C for 15 minutes.
Step 3: Phase B is added to phase A and the mixture was homogenized at 5000 RPM for 15-20 minutes.
Step 4: Phase C ingredients was mixed with 1% water and added to step 3 mixture. The mixture was stirred for 5 min at 500 RPM.
Step 5: Phase D ingredients was added to step 4 mixture and stirred for 5 min at 500 RPM
Step 6: Phase E ingredient was added to the above and mixed for 5 min at 300 RPM.
Step 6: Phase F ingredient was added to the above and mixed for 5 min at 300 RPM.
Step 7: Phase G ingredient was added to step 6 mixture and mixed for 5 min at 300 rpm to complete the batch.

Example 2
An anti-aging facial hydrogel formulation in accordance with the present invention can have the composition as mentioned in Table 2 below
Table 2: An anti-aging facial hydrogel comprising aqueous extract of Kappaphycus and naringenin
Phase Name of ingredient % wt Functionality
A Water 85.73
A Disodium EDTA 0.05 Chelant
A Carbomer 0.05 Structurant
A Xanthum gum 0.10 Structurant
A Glycerine 3.00 Humectant
A Butylene glycol 2.00 Humectant
B Caprylic/Capric triglyceride 5.00 Emollient
B Isopropyl myristate 2.00 Emollient
B Dimethicone 1.00 Feel enhancer
C Triethanol amine 0.08 Neutralizer
D Kappaphycus extract 0.45 Active
D Naringenin 0.15 Active
E Phenoxyethanol 0.40 Preservative

The above hydrogel was prepared by the process as mentioned below
Step 1 all ingredients in Phase A were weighed and dispersed and stirred for 15 min at 300 rpm.
Step 2 ingredients of Phase B are weighed and mixed and added to above mix and stirred for 10 min at 300 rpm.
Step 3 the above mixture was neutralized using phase C and stirred for 10 min at 400 rpm.
Step 4 Phase D ingredients were weighed and added to the above and stirred for 5 min at 300 rpm.
Step 5 the batch was completed by adding phase E and stirred for 5 min at 300 rpm.

Example 3
Experiments to show the synergistic activity of combination of Kappaphycus extract and naringenin in accordance with the present invention
Sample composition
Step 1: Kappapycus extract was weighed in a beaker and appropriate amount of water (100 ml) was added to make the final concentration as 1%;
Step 2: Naringenin (Sigma) was weighed into another beaker and appropriate amount of water (100 ml) was added to make the final concentration as 1%;
Step 3: The contents were allowed to dissolve completely in room temperature or vortexed if needed;
Step 4: All the contents were transferred to a container and stored at 2-8°C.
Different samples were prepared in accordance with the above method by varying the combining ratio of Kappaphycus and naringenin as mentioned in Table 3.

Experimental details
Countable number of C. elegans (~20) was dispensed inside individual wells of microtiter plate containing M9 buffer or distilled water. The total reaction volume was 500µl. The individual active compounds were added in such a way that the final concentration of the compound inside the mix will be 0.01%.
In the case of synergistic activity, the total volume of the active compound inside a well was made as 0.01%. In other words, the combined concentration of both the compounds inside the well was 0.01%.
The C. elegans were counted manually through a Steriomicroscope at every 8 hour interval. C. elegans were considered dead when they did not respond to a gentle tap of the plate or to a touch with a platinum wire pick. Each experimental condition was done in triplicates and the rate of survival was plotted graphically.

Table.3 depicts the synergistic activity of combination of Kappaphycus and naringenin.
No. of days survived by C. Elegans
Control Kappaphycus Naringenin Combination ratio (Kappaphycus: Naringenin) Longevity Synergistic activity
21 23 23 1:1 25 Yes
2:1 27 Yes
3:1 25 Yes
4:1 24 Yes
5:1 21 No
1:2 29 Yes
1:3 27 Yes
1:4 21 No
1:5 18 No

From Table.3, it is observed that the combination of Kappaphycus extract and Naringenin at specific ratio of 1:3 to 4:1 synergistically enhances the longevity of C. elegans as compared to the Kappaphycus extract and naringenin when taken alone or in other combination such as 5:1, 1:4, and 1:5.

Example 4
Experiments to show the synergistic activity of combination of kappaphycus extract and naringenin with UV exposure in accordance with the present invention
C. elegans and the active compounds were dispensed to the microtitre plate as mentioned in Example 3. Then the plate was exposed to UV at 365 nm constantly for 3 hours.

Table.4 depicts the synergistic activity of Kappaphycus and Naringenin combination with UV exposure.
No. of days survived by C. elegans when exposed to UV rays
Control Kappaphycus Naringenin Combination ratio (Kappaphycus: Naringenin) Longevity Synergistic activity
17 18 29 1:1 20 Yes
2:1 23 Yes
3:1 25 Yes
4:1 21 Yes
5:1 17 No
1:2 21 Yes
1:3 23 Yes
1:4 19 No
1:5 16 No

From Table.4, it is observed that the combination of Kappaphycus and Naringenin at specific ratio of 1:3 to 4:1 synergistically enhances the longevity of C. elegans in presence of UV light compared with the Kappaphycus and Naringenin taken alone or in other combination such as 5:1, 1:4, and 1:5.

Example 5
The combination of two molecules always do not extend the lifespan or work synergistically, each known to extend the lifespan of C. elegans organisms. This phenomenon is illustrated with the example of Folic acid and Quercetin combination in ratio of 1:1 (Total concentration of actives maintained at 0.01%).

Experimental details were the same as adopted in example 3. The active molecules used were dilute solutions (1% aqueous) of querectin and green tea.

Table.5 depicts the effect of combination of Folic acid and Quercetin in ratio of 1:1 on lifespan of C.elegans.
Life-span of C. elegans (in days)
Control Folic acid Quercetin 1:1 Folic acid and Quercetin
20 22 24 < 2 days (around 36 hrs)

From Table.5, it is observed that the combination of Folic acid and Quercetin in a ratio of 1:1 drastically shorten the longevity of C. elegans organisms to less than 2 days (36 hours) compared with the Control, Folic acid and Quercetin alone. In fact the combination of Folic acid and Quercetin are antagonistic to each other.