Description:Title of The Invention
Plant derived antifeedant agents against polyphagous pest spodoptera litura
Field of the Invention
This invention relates to plant derived antifeedant agents.
.Background of the Invention
US2015/0196036A1: The investigation provides use of plant extract namely A. vulgaris, C. maculatum, H. lupulus, L.temulentum, S. nigra, R. tinctoria, U.dioica, S. officinalis, X.strumarium, R. lutea, C. album, S.nigrum, for insecticidal activity against Leptinotarsa decemlineata.
Research Gap: The plants extract used for study are S. scandens and H. suveolens against Spodoptera litura a polyphagous pest.the study was not conducted earlier
WO2004/049808A1: This invention relates to the field of microbial pest control. Provided is a biopesticide composition comprising a bacterium of the genus Serratia, wherein this bacterium is associated with a plant or endophytic; does not cause a systemically acquired resistance in a plant; and does not excrete a toxic compound in the medium when cultured. A method is provided to control or kill an undesired organism, comprising contacting an undesired organism, a plant and / or soil with an amount of the biopesticide composition according to the invention that is sufficient to control or kill said organism. Use of such a method can control or prevent a disease caused by undesired organisms, such as thrips and mites.
Research Gap: The plants extract used for study are S. scandens and H. suveolens against Spodoptera litura a polyphagous pest study was not conducted earlier.
WO2009/093257A2: The present invention relates to a biopesticide based on or more entomopathogenic fungi which is formulated as a novel highly compressed tablet and combinations thereof. The formulation exhibits longer shelf life and lesser contamination by saprophytic fungi. It also relates to a process of preparing the biopesticide formulation and modes of application of the same.
Research Gap: Plant extracts of S. scandens and H. suveolens tested against study against Spodoptera litura. The study was not conducted earlier.
None of the prior art indicate above either alone or in combination with one another disclose what the present invention has disclosed. Present invention is two plants Scutellaria scandens (Lamiaceae) and Hyptis suveolens (Lamiaceae) were collected in flowring season from Uttarakhand Himalaya. The collected plant material was dried in shade and then extracted with 80% ethanol using soxhlet apparatus. The obtained extract is partitioned using n-hexane, ethyl acetate, methanol and water.
SUMMARY OF THE INVENTION
This summary is provided to introduce a selection of concepts, in a simplified format, that are further described in the detailed description of the invention.
This summary is neither intended to identify key or essential inventive concepts of the invention and nor is it intended for determining the scope of the invention.
To further clarify advantages and features of the present invention, a more particular description of the invention will be rendered by reference to specific embodiments thereof.
Spodoptera lituraL (Lepidoptera), a polyphagous pest of cotton rice, tomato, tobacco, groundnut, castor and legumes. In larval stage it causes several damage to different crops from ancient times, pesticides have been usd by humans to prevent damages of the crops. Sulphur was the first known pesticides. Toxic chemicals present in pesticides like Hg, As & Pb are responsible for killing crop pests. In today’s scenario crop protection technologies depends mainly on synthetic pesticides. Uninterrupted and massive use, however has led to several surprising side effects such as pest resistance removal of bio control agents of natural origin, harmful effect on non-target organism and contamination of environment which include soil contamination, ground water contamination, soil contamination and also effect food chain. It also leads to cancer, endocrine disruption, obesity and many other diseases in human. Plant based pesticides are a lot more secure to higher creatures, including human beings. A few weight have quickened the quest for all the more ecologically and toxicologically safe and selective and efficacious pesticides.
A method of preparation of Plant derived antifeedant agents of Scutellaria scandens (Lamiaceae) and Hyptis suveolens (Lamiaceae) against polyphagous pest spodoptera litura comprising the steps of collecting plant material is dried in shade and then extracted with 80% ethanol using soxhlet apparatus; obtaining extract is partitioned using n-hexane, ethyl acetate, methanol and water; fractions were analysed for antifeedant activity Spodoptera lituraL (Lepidoptera), a polyphagous pest of cotton rice, tomato, tobacco, groundnut, castor and legumes are used as test insect for antifeedant studies.
The Larvae of the pest are collected from field and cultured on caster leaves in lab; third instar larvae are used for the activity; the different extract alone and in combination are tested against third instar larvae of Spodoptera litura by leaf dip method.
The collected plant material was dried in shade and then extracted with 80% ethanol using soxhlet apparatus; obtained extract is partitioned using n-hexane, ethyl acetate, methanol and water; fractions were analysed for antifeedant activity Spodoptera lituraL (Lepidoptera), a polyphagous pest of cotton rice, tomato, tobacco, groundnut, castor and legumes] were used as test insect for antifeedant studies.
The method as claimed in claim 1, wherein said Larvae of the pest were collected from field and cultured on caster leaves in lab.
The method as claimed in claim 1, wherein said third instar larvae were used for the activity; the different extract alone and in combination were tested against third instar larvae of Spodoptera litura by leaf dip method.
A composition is prepared, wherein composition comprises Scutellaria scandens (Methanol: water extract) (2:1) 10.1±2.6 (Average larval wt. mg).
DETAILED DESCRIPTION OF THE INVENTION
The detailed description of various exemplary embodiments of the disclosure is described herein with reference to the accompanying drawings. It should be noted that the embodiments are described herein in such details as to clearly communicate the disclosure. However, the amount of details provided herein is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the scope of the present disclosure as defined by the appended claims.
It is also to be understood that various arrangements may be devised that, although not explicitly described or shown herein, embody the principles of the present disclosure. Moreover, all statements herein reciting principles, aspects, and embodiments of the present disclosure, as well as specific examples, are intended to encompass equivalents thereof.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments. As used herein, the singular forms “a",” “an” and “the” are intended to include the plural forms as well, unless the context clearly indicates otherwise. It will be further understood that the terms “comprises,” “comprising,” “includes” and/or “including,” when used herein, specify the presence of stated features, integers, steps, operations, elements and/or components, but do not preclude the presence or addition of one or more other features, integers, steps, operations, elements, components and/or groups thereof.
It should also be noted that in some alternative implementations, the functions/acts noted may occur out of the order noted in the figures. For example, two figures shown in succession may, in fact, be executed concurrently or may sometimes be executed in the reverse order, depending upon the functionality/acts involved.
In addition, the descriptions of "first", "second", “third”, and the like in the present invention are used for the purpose of description only, and are not to be construed as indicating or implying their relative importance or implicitly indicating the number of technical features indicated. Thus, features defining "first" and "second" may include at least one of the features, either explicitly or implicitly.
Unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which example embodiments belong. It will be further understood that terms, e.g., those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
These and other advantages of the present subject matter would be described in greater detail with reference to the following figures. It should be noted that the description merely illustrates the principles of the present subject matter. It will thus be appreciated that those skilled in the art will be able to devise various arrangements that, although not explicitly described herein, embody the principles of the present subject matter and are included within its scope.
Plant Material: Two plants Scutellaria scandens (Lamiaceae) and Hyptis suveolens (Lamiaceae) were collected in flowring season from Uttarakhand Himalaya.
Extraction and Isolation: The collected plant material was dried in shade and then extracted with 80% ethanol using soxhlet apparatus. The obtained extract is partitioned using n-hexane, ethyl acetate, methanol and water. The fractions were analysed for antifeedant activity.
a) Determination of antifeedant activity
Testing material: Spodoptera lituraL (Lepidoptera), a polyphagous pest of cotton rice, tomato, tobacco, groundnut, castor and legumes] were used as test insect for antifeedant studies. Larvae of the pest were collected from field and cultured on caster leaves in lab.Third instar larvae were used for the activity.
The different extract alone and in combination were tested against third instar larvae of Spodoptera litura by leaf dip method. Extracts were dissolved in small quantity of ethanol and diluted with water containing 0.05% Triton X100.small leaf disc of castor leaves were prepared and dipped in each extract for around 20 sec. the leaf discs dipped only in water is taken as control. On dried leaf discs 5 larvae of pest were released. Three replicates were maintained. Larval weight was taken after 5 days of treatment.
Percent Growth Reduction and Mortality was calculated in comparison to control
Hexane, ethyl acetate, methanol and aqeous fractions in different ratio of Scutellaria scandens and Hyptis suveolens were tested against Spodoptera litura (Lepidoptera) a polyphagous pest of cotton, rice, tomato, groundnut, and caster etc by Leaf Dip Method and results are given in table 1.
S.No. Particular Average larval wt. mg Growth reduction (%)
1. Scutellaria scandens (hexane:ethyl acetate ) (1:1) 14.5±1.1 35.74
2. Scutellaria scandens (Methanol: water extract)(2:1) 10.1±2.6 54.33
3. Hyptis suveolens (Hexane:ethyl acetate ) (1:1) 15.9±0.62 32.74
4. Hyptis suveolens(Methanol:water extract)(2:1) 9.2±2.4 44.32

Examples 1:
A method of preparation of Plant derived antifeedant agents of Scutellaria scandens (Lamiaceae) and Hyptis suveolens (Lamiaceae) against polyphagous pest spodoptera litura comprising the steps of collecting plant material is dried in shade and then extracted with 80% ethanol using soxhlet apparatus; obtaining extract is partitioned using n-hexane, ethyl acetate, methanol and water; fractions were analysed for antifeedant activity Spodoptera lituraL (Lepidoptera), a polyphagous pest of cotton rice, tomato, tobacco, groundnut, castor and legumes are used as test insect for antifeedant studies.
The Larvae of the pest are collected from field and cultured on caster leaves in lab; third instar larvae are used for the activity; the different extract alone and in combination are tested against third instar larvae of Spodoptera litura by leaf dip method.
The collected plant material was dried in shade and then extracted with 80% ethanol using soxhlet apparatus; obtained extract is partitioned using n-hexane, ethyl acetate, methanol and water; fractions were analysed for antifeedant activity Spodoptera lituraL (Lepidoptera), a polyphagous pest of cotton rice, tomato, tobacco, groundnut, castor and legumes] were used as test insect for antifeedant studies.
The method as claimed in claim 1, wherein said Larvae of the pest were collected from field and cultured on caster leaves in lab.
The method as claimed in claim 1, wherein said third instar larvae were used for the activity; the different extract alone and in combination were tested against third instar larvae of Spodoptera litura by leaf dip method.
A composition is prepared, wherein composition comprises Scutellaria scandens (Methanol: water extract) (2:1) 10.1±2.6 (Average larval wt. mg).

Claims:1. A method of preparation of Plant derived antifeedant agents of Scutellaria scandens (Lamiaceae) and Hyptis suveolens (Lamiaceae) against polyphagous pest spodoptera litura comprising the steps of collecting plant material is dried in shade and then extracted with 80% ethanol using soxhlet apparatus; obtaining extract is partitioned using n-hexane, ethyl acetate, methanol and water; fractions were analysed for antifeedant activity Spodoptera lituraL (Lepidoptera), a polyphagous pest of cotton rice, tomato, tobacco, groundnut, castor and legumes are used as test insect for antifeedant studies.
2. The method as claimed in claim 1, wherein Larvae of the pest are collected from field and cultured on caster leaves in lab; third instar larvae are used for the activity; the different extract alone and in combination are tested against third instar larvae of Spodoptera litura by leaf dip method.
3. The method as claimed in claim 1, wherein said collected plant material was dried in shade and then extracted with 80% ethanol using soxhlet apparatus; obtained extract is partitioned using n-hexane, ethyl acetate, methanol and water; fractions were analysed for antifeedant activity Spodoptera lituraL (Lepidoptera), a polyphagous pest of cotton rice, tomato, tobacco, groundnut, castor and legumes] were used as test insect for antifeedant studies.
4. The method as claimed in claim 1, wherein said Larvae of the pest were collected from field and cultured on caster leaves in lab.
5. The method as claimed in claim 1, wherein said third instar larvae were used for the activity; the different extract alone and in combination were tested against third instar larvae of Spodoptera litura by leaf dip method.
6. A composition prepared by the method as claimed in claim 1-5, wherein composition comprises Scutellaria scandens (Methanol: water extract) (2:1) 10.1±2.6 (Average larval wt. mg).