CLIAMS:1] A plant growth promoter composition comprising synergistically effective amounts of
 0.05 to 0.20 ml/L fermented seaweed extract as source of proteins, phyto-harmones, nutrients and trace elements beneficial for plant growth, said extract further comprising ethanol for maintenance of bacteriostatic conditions in the plant growth promoter composition, nutrient broth to act as carbon and nitrogen source for the fermentative bacteria and distilled water for solubilization of ingredients in the plant growth promoter composition;
 0.01 to 0.03 ml/L of emulsifier for promoting homogenous mixing of the plant growth promoter composition;
 0.0005 to 0.002 g/L of formalin as an anti-contamination and stabilizing agent;
 0.0005 to 0.002 g/L of Folic Acid from sources selected among beetroot, spinach and synthetic Folate as a source of multivitamins, nutrients, participation in plant metabolic and anabolic processes.
2] The plant growth promoter composition according to claim 1, wherein said emulsifier is Nonyl Phenol Ethylene Oxide Condenset
3] The plant growth promoter composition according to claim 1 which results in accelerating growth rate of plants when applied via foliar spray in concentration of 15 ml in 15 lit. water
4] The plant growth promoter composition according to claim 1, which is applied to plants in their growth and development stage.
5] The plant growth promoter composition according to claim 1, which promotes the growth of the leaves, stems, roots, tubers, rhizomes or fruits of a plant, or the differentiation of the flower buds of a plant
6] The plant growth promoter composition according to claim 1, wherein the seaweed extract is obtained by fermenting sea weed substrate using a pure culture of Bacillus Megaterium.
7] A method for preparing the plant growth promoter composition of claim 1, comprising
 adding 200kg of brown seaweed in 100 liters of water in an agitator vessel and stirring the admixture for 24 hours at 50 to 60oC temperature and subsequently cooling the contents to room temperature to obtain an aqueous seaweed extract base;
 inoculating 0.5ml of Bacillus Megaterium suspension in 250ml volume flask containing 50ml growth media and subjecting the same to incubation for 48 hours at 120 RPM on rotary shaker to obtain a first pure culture suspension;
 inoculating 2ml of the first pure culture suspension in 1000ml volume flask containing 200ml media and subjecting the same to incubation on rotary shaker for 24 hours to obtain a seed culture;
 inoculating 5 L of the seed culture to the aqueous seaweed extract base and allowing fermentation over 48 hours to obtain a first fermentation product;
 Admixing ethanol to the first fermentation product while continuous stirring over 30 minutes to introduce bacteriostatic conditions and obtain a stabilized fermentation product;
 Clarifying the stabilized fermentation product through an agitated Nustche filter to separate the residue and obtain a clarified filtrate base; and
 Admixing 0.05 to 0.20 ml/L of clarified filtrate base, 0.01 to 0.03 g/L of Nonyl Phenol Ethylene Oxide Condenset, 0.0005 to 0.002 g/L of formalin and 0.0005 to 0.002 g/L of Folic Acid and stirring well for homogeneity to constitute the final plant growth promoting composition ,TagSPECI:[001] FIELD OF THE INVENTION
[002] The present invention relates to a plant growth-promoter and more specifically to an herbal plant growth promoting agent comprising concentrate prepared from fermentation of marine algae additionally contains cystine precursors and other amino acids.

[003] BACKGROUND OF THE PRESENT INVENTION.
[004] In the utilization of plants, including in agricultural production, it is always a concern for users of plants how efficiently a plant is grown. Promotion of ordinary growth and development of crops for their increased production has been a big task in the agricultural technology. This task is becoming more important because of food shortage on global scale which is anticipated to increase in the future.

[005] A method for achieving the task includes a method by a technique employing an agricultural facility capable of controlling the temperature, light and other factors determined to be necessary for optimum growth of plants. This method, however, has had problems in that costly facilities and equipment therefor are required and that the method has a limitation as to area of implementation and control.

[006] Growth promotion of plant bodies in an aerial part means to promote the formation of stems which are resistant to winds and rains and can accelerate development of leaves to promote photosynthesis. The growth promotion of roots can spread out the roots to support the aerial part of the plant such as stems, leaves, flowers and so on and allow the roots to absorb nutritional components in a wide range. In particular, growth promotion is extremely important in an early phase of growth.

[007] Efforts to effectively grow plants have been made conventionally. Eswaran, et al in US 6,893,479 disclose integrated method is developed to utilize to a maximum extent the fresh biomass of seaweeds such as Kappaphycus alvarezii that can be crushed to release sap and where the sap is useful as a potent liquid fertilizer after suitable treatment with additives. In methods of using substances having the action of promoting plant growth, e.g., plant hormone preparations such as ethylene, gibberellins, auxins and activators of particular enzymes in the plant body, many problems remain, e.g., the concentration disorder for plants, safety for the environment, especially for animals or plants other than the target plants, safety for the human body, soil pollution, change in soil pH, simplicity of procedure, or utilization and costs. Thus, the development of a plant growth-promoting agent in which those problems have been solved is required.

[008] There have recently been made attempts of isolating a physiologically active substance from plant tissue samples and studying its effect on the life cycle (germination, growth, blooming, bearing and aging) of plants in order to promote their growth and development for increased production. However, the number of cases is very small yet in which a physiologically active substance having a plus effect on the growth and development of plants has been put into practical use. Moreover, the number of substances is also very small which can exhibit a growth promotion action on plants in their actual outdoor cultivation.

[009] These, along with other encumbrances of the art have been focus of research of the present inventor, who has come up with novel solutions to address the same. The following detailed description presents one way of performing the present invention.

[010] OBJECTS OF THE PRESENT INVENTION
[011] The principle object of the present invention is to provide for a natural plant growth promoting agent that owes its constitution to natural organic ingredients and avoids incorporation of synthetic chemicals.

[012] Yet another object of the present invention is to provide for a plant-growth promoting agent which can be stored in a stable state.

[013] Yet another object of the present invention is to provide for a plant growth-promoting agent which can be dispersed and stably present in a medium such as a soil or an aqueous liquid which allows mass transport of nutrition to the plant.

[014] Yet another object of the present invention is to provide for a plant growth-promoting agent which is effective also at early stages in plant growth.

[015] Yet another object of the present invention is to provide for a plant growth-promoting agent which is environmentally safe and are substantially non-toxic to large animals

[016] Yet another object of the present invention is to provide for a plant growth-promoting agent which is characterized in low costs of raw materials, method of formulation and use.

[017] Yet another object of the present invention is to provide for a plant growth-promoting agent which is safe for both the user and environment of use and does not result in any toxic effects or risk of bio-magnification.

[018] These objects, together with other objects and advantages which will become subsequently apparent, reside in the details of preparation and application as more fully hereinafter described and claimed, reference being had to the accompanying drawings and detailed description set forth hereinafter

[019] SUMMARY OF THE PRESENT INVENTION
[020] The present invention is directed to a plant growth promoting composition comprising plant macronutrients, micronutrients, a pest inhibitor and at least one growth regulator in addition to a penetrant and an energy source. The proposed plant growth promoting composition comprises sea weed, formalin, methanol, emulsifier, folic acid and nutrient broth and at least in one aspect is applied to the plant via foliar spray.

[021] BRIEF DESCRIPTION OF THE DRAWINGS
[022] Table 1a illustrates a preferred composition of plant growth promoting agent of the present invention
[023] Table 1b illustrates composition of broth used for inoculums development.
[024] Table 2 illustrates test design for bio-efficacy studies in Okra crop
[025] Table 3 illustrates test statistics for promotion of plant height in Okra upon application of plant growth promoting agent of the present invention
[026] Table 4 illustrates test statistics for promotion of branching in Okra upon application of plant growth promoting agent of the present invention
[027] Table 5 illustrates test statistics for promotion of stem diameter bulk in Okra upon application of plant growth promoting agent of the present invention
[028] Table 6 illustrates test statistics for acceleration of initiation and flowering in Okra upon application of plant growth promoting agent of the present invention
[029] Table 7 illustrates test statistics for promotion of biomass in Okra upon application of plant growth promoting agent of the present invention
[030] Table 8 illustrates test statistics for promotion of absolute growth rate in Okra upon application of plant growth promoting agent of the present invention
[031] Table 9 illustrates test statistics for promotion of crop growth rate in Okra upon application of plant growth promoting agent of the present invention
[032] Table 10 illustrates test statistics for increase in leaf area index in Okra upon application of plant growth promoting agent of the present invention
[033] Table 11 illustrates test statistics for increase in chlorophyll content in Okra upon application of plant growth promoting agent of the present invention
[034] Table 13a and 13 b illustrate test statistics for analysis of soil samples in test bed of Okra before and after experimental application of plant growth promoting agent of the present invention respectively
[035] Table 12 illustrates test statistics for yield and yield components for Okra crop upon application of plant growth promoting agent of the present invention
[036] Table 14 illustrates test statistics for appraisal of qualitative parameters in Okra crop upon application of plant growth promoting agent of the present invention
[037] Table 15 illustrates spraying / application schedules of the plant growth promoting agent of the present invention for different plant and crop varieties

[038] DETAILED DESCRIPTION OF THE INVENTION
[039] In view of the foregoing disadvantages inherent in the state of art, the present invention is directed towards provision of a plant growth-promoting agent which is highly safe and exhibits an excellent plant growth-promoting effect under wide atmospheric conditions.

[040] In this respect, before explaining at least one embodiment of the invention in detail, it is to be understood that the invention is not limited in its application to the details of construction and to the arrangements of the components set forth in the following description or illustrated in the drawings. The invention is capable of other embodiments and of being practiced and carried out in various ways. Also, it is to be understood that the phraseology and terminology employed herein are for the purpose of description and should not be regarded as limiting. As such, the general purpose of the present invention is to incorporate all the advantages of the prior art and none of the disadvantages.

[041] Compositions according to the present invention can comprise one or more macronutrients. For purposes of the present description, the term “macronutrient” can refer to an element for plant growth which is utilized by plants in proportionally larger amounts relative to micronutrients. For most plant species and for purposes of the present description, macronutrients include nitrogen, potassium, phosphorus, calcium, magnesium and sulfur. Compositions of the present invention can include various combinations and relative amounts of individual macronutrients. Preferably, compositions include both phosphorous and potassium. In particular embodiments, compositions of the present invention include each of the listed macronutrients. Table 1 lists one exemplary formulation of the nutrient composition of the present invention:

[042] In a broad sense, the products within the scope of the present invention may take a variety of forms. For instance, the product may be manufactured and sold as a ready-for-use premix or concentrate for use post due dilution. Alternatively, the product may be in the form of desiccated formulations for use upon reconstitution and the like administrables. Accordingly, auxiliary agents such as carriers, emulsifiers, dispersing agents, spreading agents, wetting and spreading agents, sticking agents, disintegrating agents and the like are integrated without departing from essence of the present invention. There is no particular restriction as to the form in which the present growth promoter is used. The form includes emulsion, suspension, powder, water-dispersible powder, water-soluble powder, granules, paste, aerosol and so on.

[043] According to another aspect of the present invention, the plant growth promoting composition proposed herein may be formulated in the stages as under:
a. Extraction: Here, 100 liters of water is taken in an agitator vessel. 200kg of seaweed is added to an extraction vessel and stirred for one day at 50 to 60oC temperature and subsequently cooled to room temperature.
b. Fermentation: Here, 0.5ml suspension of Bacillus Megaterium is inoculated in 250ml volume flask containing 50ml growth media and incubated for 48 hours at 120 RPM on rotary shaker. After checking growth for purity and biomass yield, 2ml of the resulting suspension is inoculated in 1000ml volume flask containing 200ml media and incubated on rotary shaker for 24 hours Growth is checked periodically. After checking growth for purity and biomass yield, 5 liter inoculants of Bacillus Megaterium broth are added to extraction vessel and fermentation undertaken for 48 hours. Protein concentration is monitored. Ethanol is added for stopping bacterial growth stirred continuously for 30 minutes.
c. Filtration: The total mass of fermentation is poured into Agitated Nustche filter. Residue is separated filtrate collected is stored in stainless steel storage tank/HDPE drum.
d. Mixing: 0.1 folic acid, Emulsifiers, Formalin are mixed in the filtrate and stirred well for homogeneity to constitute the final plant growth promoting composition of the present invention.

[044] Accordingly, the inventive plant growth promoting composition is finally formulated in the stoichiometry presented in Table 1a. Table 1.b illustrates growth medium needed for inoculum development and growth of Bacillus Megaterium.

[045] According to principles of the present invention, the plant growth promoting composition above described may be formulated as water soluble concentrate and used at all stages of plant growth. Heat or water stress, growth stages of crop, reduction of flower and fruit drop, spraying other products for synergistic effects are events in which the said novel product finds efficient applicability. When sprayed on leaves, it is readily absorbed and transmitted to all parts of the plant. Simple proteins and other nutrients contained in said composition are easily utilized by the plants and thus, physiology of the plant system is positively influenced with marked tonic / growth promoter effect

[046] BEST MODE FOR CARRYING OUT THE INVENTION
[047] It shall be evident to the reader that during application of composition proposed herein, the relative concentrations of various ingredients are utmost critical and if outside optimized ranges, their inclusion is not economical, the growth promotion effect does not manifest and in some rare cases, even growth inhibition effect may be seen. Thus, the relative concentrations of the ingredients comprising the plant growth promoter proposed herein are not devised by mere trial and error but with complete understanding of plant physiology and chemistry. The present invention is hereinafter described more specifically by way of an example in which quantities in parts and % are each by weight unless otherwise specified.

[048] Example 1: Growth promotion in Abelmoschus esculentus
[049] The growth promoter of the present invention exhibits a growth promotion action to Okra (Abelmoschus esculentus). The timing and method at and in which the growth promoter of the present invention is used, are more of less definite by spraying or coating said growth promoter on a plant in the growth and development stage or by dipping seeds of a plant in said growth promoter. In the present example, a synergistic plant growth promotion effect is obtained on Abelmoschus esculentus plant applied with the proposed plant growth promoter via foliar spray method. Table 2 showcases an exemplary trial design for testing bio-efficacy of the plant growth promoting composition proposed herein. Tables 3 to 14 showcase proof of working of the plant growth promoting composition proposed herein as admeasured from appraisal of various parameters such as plant height, branching, stem diameter bulk, acceleration of initiation and flowering, biomass, absolute growth rate, crop growth rate, leaf area index, chlorophyll content, soil content, yield and yield components, other qualitative/ aesthetic parameters respectively which each showcase a significantly alleviated and desirable appreciation.

[050] From the results conveyed above, it can be seen that the site of plant at which the effect of the present growth promoter is expressed differs depending upon the kind of plant, for example, leaf, stem, root, tuber, rhizome, fruit and flower bud. Thus, the present invention can promote plant growth. Table 15 illustrates spraying / application schedules of the plant growth promoting agent of the present invention for different plant and crop varieties which may be followed during their cultivation.

[051] The invention described and claimed herein is not to be limited in scope by the specific embodiments herein disclosed, since these embodiments are intended as illustrations of several aspects of the invention. Any equivalent embodiments are intended to be within the scope of this invention. Indeed, various modifications of the invention in addition to those shown and described herein will become apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims