Plasmid Having Three Complete Transcriptional Units And Immunogenic Compositions For Inducing An Immune Response To Hiv

Abstract: The invention describes an immunogenic composition for inducing an immune response to human immunodeficiency virus (HIV) in a vertebrate host, said immunogenic composition comprising: a) a first DNA plasmid comprising a single transcriptional unit comprising a nucleotide sequence that encodes an HIV gag-pol fusion polypeptide, wherein said single transcriptional unit is operably linked to regulatory elements including a promoter and a polyadenylation signal;(b) a second DNA plasmid comprising (i) a first transcriptional unit comprising a nucleotide sequence that encodes an HIV nef-tat-vif fusion polypeptide operably linked to regulatory elements including a first promoter and a first polyadenylation signal; (ii) a second transcriptional unit comprising a nucleotide sequence that encodes an HIV envelope polypeptide operably linked to regulatory elements including a second promoter and a second polyadenylation signal; wherein said first and second promoters are each derived from different transcriptional units; and wherein said first and second polyadenylation signals are each derived from different transcriptional units; and wherein the direction of transcription for said first transcriptional unit is in the opposite direction from the direction of transcription of said second transcriptional unit; or wherein the direction of transcription for said first transcriptional unit is in the same direction from the direction of transcription of said second transcriptional unit and said first and second transcriptional units are separated by a spacer region of at least one kilobase pairs; and (c) at least one of a pharmaceutically acceptable diluent, carrier or transfection facilitating agent.

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