DESC:FORM-2
THE PATENTS ACT, 1970
(39 of 1970)
&
THE PATENTS RULES, 2003
COMPLETE SPECIFICATION
(See Section 10 and rule 13)
Title: PROCESS FOR ENRICHMENT OF CALCIUM IN ARJUNA BARK POWDER
APPLICANT DETAILS:
(a) NAME: Zywie Ventures Pvt Ltd.
(b) NATIONALITY: IN
(c) ADDRESS: 5, Dashrath Singh Estate, Caves Road, Near Jogeshwari Caves,
Jogeshwari East, Mumbai 400060 India.
PREAMBLE TO THE DESCRIPTION:
The following specification particularly describes the nature of this invention and the manner in which it is to be performed.
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PROCESS FOR ENRICHMENT OF CALCIUM IN ARJUNA BARK POWDER
FIELD OF INVENTION
The present invention provides an efficient process for enrichment of calcium in Arjuna bark powder. The process is environment friendly, waste-free and highly efficient in increasing the calcium content in Arjuna bark powder.
BACKGROUND OF THE INVENTION
The following background discussion includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art.
Plant sources that are naturally rich in bio-available calcium are limited. Commonly recommended plant sources of calcium include kale, legumes, figs, bok choy, and broccoli. According to published literature, the quantity and bio-availability of calcium within these foods is far lower than dairy products or calcium fortified foods.
Factors in the human body can also influence calcium bio-availability. Calcium is absorbed across the gut by vitamin D dependent active transport and facilitated diffusion. Therefore, an individual’s vitamin D levels can affect calcium absorption. Factors such as sex, age, and individual calcium stores affect the rate of facilitated diffusion.
It is commonly suggested that people who do not consume animal products, particularly dairy, should eat foods fortified with calcium or take a calcium and vitamin D supplement to meet the recommended daily allowance (RDA) for this mineral. Thus, plant derived calcium is a more sustainable way as compared to dairy to get the daily calcium fix.
Hence, there is a need of plant derived calcium enriched dietary composition and an efficient method of enriching calcium content in a plant material/ parts. Particularly, there is a need of plant derived calcium enriched dietary composition and an efficient method of enriching calcium content in arjuna bark or powder thereof.
OBJECTIVE OF THE PRESENT INVENTION
An objective of the present invention is to provide an efficient process for enrichment of calcium in arjuna bark powder.
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An objective of the present invention is to provide an environment friendly, waste-free process for enrichment of calcium in arjuna bark powder.
An objective of the present invention is to provide a dietary composition comprising calcium enriched extract of arjuna bark powder.
An objective of the present invention is to provide a plant derived calcium enriched dietary composition.
Summary of the Invention:
In an aspect, the Invention provides a method of processing arjuna bark to obtain calcium enriched arjuna bark powder comprising the steps of:
a) Extraction of Arjuna bark powder with aqueous alcohol wherein the aqueous alcohol comprises water and alcohol in 50:50 ratio;
wherein extraction temperature is 70 degrees and the extraction is performed for approx. 6Hr. each wash;
b) Drying the exhausted material obtained from step (a) in a tray dryer to obtain the calcium enriched arjuna bark powder.
In an embodiment, the extraction is performed for 3 cycles.
In an embodiment, the extraction solvent comprises aqueous Methanol in 50:50 ratio.
In another aspect, the invention provides a plant derived calcium enriched dietary composition comprising calcium enriched arjuna bark powder.
DETAILED DESCRIPTION OF DRAWING:
The accompanying drawings, which are incorporated in and constitute a part of this disclosure, illustrate exemplary embodiments and, together with the description, explain the disclosed principles. The reference numbers are used throughout the figures to describe the features and components. Some embodiments of system and/or methods in accordance with embodiments of the present subject matter are now described, by way of example only, and regarding the accompanying figures, in which:
Figure 1: illustrates an overview of the process provided by the present invention
DESCRIPTION OF THE INVENTION:
In the present document, the word "exemplary" is used herein to mean "serving as an example, instance, or illustration." Any embodiment or implementation of the present subject
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matter described herein as "exemplary" is not necessarily to be construed as preferred or advantageous over other embodiments.
While the disclosure is susceptible to various modifications and alternative forms, specific embodiment thereof has been shown by way of example in the drawings and will be described in detail below. It should be understood, however that it is not intended to limit the disclosure to the specific forms disclosed, but on the contrary, the disclosure is to cover all modifications, equivalents, and alternative falling within the spirit and the scope of the disclosure.
The present invention provides an efficient process for the enrichment of calcium in arjuna bark powder. The process is environment friendly, waste-free and highly efficient in increasing the calcium content in arjuna bark powder.
The process is efficient to remove 50% Hydro-methanolic impurities from the arjuna bark powder at optimum temperature.
In an embodiment, the process comprises treatment of Arjuna Bark/Chal with organic solvents comprising combination of plurality of alcohol, water and other solvents in a specific ratio.
In an embodiment, the alcohol comprises ethanol, methanol etc.
In an embodiment, the process comprises charging approx. 30kg of Arjuna bark charge in Vertical extractor followed by extraction with aqueous Methanol at extraction temp. of 70 degree Celsius and extraction time of 6Hr. each wash.
In an embodiment, approx. 1200 lit of aqueous Methanol is used for washing the bark material. The exhausted Material is further dried in tray dryer. The output of the exhausted material was obtained as 22 kg.
The process is highly efficient and uses environment friendly solvents e.g. aqueous methanol. The test studies and experiments shows that the calcium content gets almost 1.5 times enriched in the Arjuna bark powder by the process provided by the present invention.
In an embodiment, the organic solvent comprises combination of water and methanol in specific ratio. Preferably, water and methanol are present in a ratio of 50:50.
The experiments were performed with various solvents/ combinations- Water 100%, 70:30 water: methanol, 50:50 water: methanol, 10:90 water : methanol for assessing its
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ability for calcium enrichment in Arjuna Bark. It was found that highest concentration of calcium was obtained in 50:50 aqueous methanol.
In another aspect of the Invention, there is provided a plant derived calcium enriched dietary composition and an efficient method of enriching calcium content in a plant material/parts.
In another aspect of the Invention, there is provided a plant derived calcium enriched dietary composition and an efficient method of enriching calcium content in arjuna bark or powder thereof.
The various factors which are involved in obtaining calcium enriched Arjuna bark powder is the specific combination of the extraction solvent, temperature and the extraction time that helps in enriching the calcium content.
Through various experiments, following parameters was found to play crucial role in the enrichment of calcium in the Arjuna bark powder.
Type of Solvent: Water: Methanol- 50:50- % Calcium14.95%. Water : Methanol (50:50) shows highest extraction capacity
Selection of temperature:
Temperature at 70 degree celsius shows the highest extraction rate.
Selection of extraction time:
The suitable extraction time is 6 hours and the calcium content after 6 hours of extraction process does not show significant increase in the calcium content. It becomes stable.
The details of the experiments are provided in the example section.
The Invention is further described with the help of non-limiting examples:
The following experiments were performed to evaluate the efficiency of the method for processing Arjuna bark to obtain calcium enriched arjuna bark powder.
Example 1:
Selection of solvent:
Various experiments have been performed with various solvents to determine the enrichment of the calcium. The following experiments shows that 50:50 water: methanol demonstrates maximum calcium enrichment.
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Reagents
Percentage of Enriched calcium content
Water 100%
12.95%
70:30 water : methanol
13.35%
50:50 water : methanol
14.95%
10:90 water : methanol
14.20%
Methanol 100%
13.50%
Water: Ethanol
12.35%
Ethanol 100%
12.05%
Example 2:
Effect of Temperature:
Various experiments have been performed to determine the effect of varying temperature. The following experiments shows that extraction temp. at 70 degrees demonstrates maximum calcium enrichment.
Reagents
Percentage of Enriched calcium content
Extraction temp. at 70 degrees
14.95%
Extraction temp. at 65 degrees
13.70%
Extraction temp. at 75 degrees
14.90%
Example 3:
Effect of Extraction time at 6 hours each wash:
The following experiments have been performed to determine the effect of extraction time. The results of various experiments shows that the most effective extraction time is 6 hours which yields maximum calcium enrichment. The calcium content after 6 hours of extraction process does not show significant increase in the calcium content. It becomes stable.
Reagents
Percentage of Enriched calcium content
Extraction time at 6 hours each wash
14.95%
Extraction time at 5 hours each wash
13.35%
Extraction time at 7 hours each wash
14.95%
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Example 4:
The method of processing Arjuna bark to obtain calcium enriched arjuna bark powder comprises following steps:
• Take 30 kg of Arjuna bark powder & place it in the vertical extractor for performing the extraction;
• The extraction solvent is water: methanol present in the ratio of 50:50 )
• The extraction is performed at 70 degree celsius for 6 hrs.
Approx.-1200 lit methanol and 1200 lit water is used for the extraction. The exhausted material is further dried in tray dryer. ,CLAIMS:We Claim:
1. A method of processing Arjuna bark to obtain calcium enriched arjuna bark powder comprising the steps of:
a) Extraction of Arjuna bark powder with aqueous alcohol wherein the aqueous alcohol comprises water and alcohol in 50:50 ratio;
wherein extraction temperature is 70 degrees and the extraction is performed for approx. 6Hr. each wash;
b) Drying the exhausted material obtained from step (a) in a tray dryer to obtain the calcium enriched arjuna bark powder.
2. The process as claimed in claim 1, wherein the extraction is performed for 3 cycles.
3. The process as claimed in claim 1, wherein the alcohol comprises methanol, ethanol.
4. The process as claimed in claim 1, wherein the aqueous alcohol comprises aqueous Methanol.
5. The process as claimed in claim 1, wherein the aqueous alcohol comprises 50:50 aqueous Methanol.
6. The process as claimed in claim 1, wherein arjuna bark is present in an amount of 30 kg.
7. The process as claimed in claim 1, wherein the aqueous methanol is present in an amount of 2400 litre.
8. A plant derived calcium enriched dietary composition comprising calcium enriched arjuna bark powder.