The present invention relates to a novel methylation process useful in the preparation of compounds which are intermediates in the synthesis of Raltegravir. The present invention also relates to an improved method for the effective acylation of an amine in the presence of a silylating agent and a catalyst and its application in the preparation of Raltegravir.

BACKGROUND OF THE INVENTION

Raltegravir is an HIV-integrase inhibitor and finds its application as an antiretroviral drug, marketed in the form of its potassium salt under the brand name Isentress® by Merck & Co. (or Merck Sharp & Dome). The chemical name for Raltegravir potassium is 4-[N-(4-fluoro-benzyl)-carbamoyl]-l-methyl-2-{ 1 -methyl- l-(5-methyl-1, 3, 4 oxadiazol-2-yl-carboxamido)ethyl}-6-oxo-l,6-dihydropyrimidin-5-olate potassium salt (Formula I). Raltegravir is disclosed in patent application WO2003035077.

WO2006060730 discloses a method for the preparation of Raltegravir (Scheme 1).

The methylation reaction onto a key intermediate is performed in the presence of magnesium methoxide, which allegedly favors the methylation on the ring nitrogen atom over the neighboring oxygen. The reaction however is not selective and treatment for the removal of the O-methylated isomer is required. According to the patent application, a mixed solvent system of methanol and t-butyl dimethyl ether is used for this purpose and still the product of the reaction contains "less than 0.5%" of the O-methylated product.

The synthesis is completed by deprotecting the amine group and subjecting the free amine to reaction with 5-methyl-l,3,4-oxadiazole-2-carbonyl chloride. Notably the

reaction with the oxadiazole requires 2.2 equivalents of the latter for full consumption of the amine (WO2006060730, p.60 line 12). The oxadiazole reagent is, however, rather expensive and it is imperative for an industrially viable process to lower the cost as much as possible.

Scheme 1

Scheme 2

WO2009088729 discloses another method for the preparation of Raltegravir, shown above (Scheme 2).

According to the disclosure of this patent application, the methylation method of WO2006060730 is accompanied by the loss of CBZ group, the demethylation of the ester and the formation of O-methylated by-products, which affects the yield of the desired N-methyl product. Allegedly, the process disclosed in WO' 729 does not include those drawbacks. However the process employs the use of N-methylpyrrolidone as a solvent and trimethylsulfoxonium iodide as the methylating agent. Additionally, it performs the reaction in high temperatures (about 100 °C) for more than 6 hours. The solvent is far from being desirable for industrial purposes and the use of methylating reagents always requires careful handling and disposal because of their very nature. Prolonged reaction time in highly elevated temperatures is also not desirable, because it renders industrial processes more energy-costing and thus less attractive from an economic aspect.

The methylation product' s free hydroxyl group is converted to its respective pivaloyl ester. Allegedly, the formation of the pivaloyl ester overcomes the problems associated with the non-protected phenol, which is difficult to dry. The required equivalents of the oxadiazole salt are reduced to about 1.15 equivalents and this allows again for full consumption of the amine (WO2009088729 lines 9-13). This modification, however, introduces a protection and a deprotection step and further requires the use of pivaloyl chloride which inevitably adds up to the cost.

Indian patent application 736/CHE/2012 suggests the use of a dehydrating agent and a base for the performance of this reaction. Allegedly this leads to lower demand for the potassium oxadiazole salt, particularly 1.35 and 1.60 equivalents according to the examples. The application, however, does not disclose the complete consumption of the starting material, which might explain the 90% molar yield obtained according to its teaching.

Therefore there exists a need for the provision of an improved process for the preparation of Raltegravir and key intermediates thereof via an effective methylation process, which can be employed in a chemo selective manner, uses less dangerous reagents and solvents and can be applied in an industrial scale with low cost. Moreover, a cost-efficient and straightforward acylation reaction of amine intermediate (compound of formula lib) is also desirable.

SUMMARY OF THE INVENTION

The present invention discloses a novel processes for preparing compounds useful as intermediates in the synthesis of Raltegravir.

There is disclosed a process for the preparation of compounds of formulae II and IV, wherein a methyl group is introduced selectively on the -NH- moiety of the heterocyclic ring, by a method which comprises: i) reaction with a halomethylhalosilane reagent S and ii) reaction with a fluoride ion source, optionally comprising further steps between them.

There is further provided a process for the preparation of Raltegravir (I) wherein a methyl group is introduced selectively on the -NH- moiety of the heterocyclic ring, according to the previous method.

There is further provided a process for the preparation of Raltegravir or a pharmaceutically acceptable salt thereof, comprising acylation of amine intermediate compound of formula lib with oxadiazole intermediate compound of formula V in the presence of a silylating agent and an acid.

DEFINITIONS

The following terms shall have, for the purposes of this application, including the claims appended hereto, the respective meanings set forth below. It should be understood that when reference herein is made to a general term, such as acid, base, oxidizing agent, etc. one skilled in the field may make appropriate selections for such reagents from those given in the definitions below, as well as from additional reagents recited in the specification that follows, or from those found in literature references in the field.

An "alkyl" group refers to any saturated aliphatic hydrocarbon, including straight-chain, and branched-chain. In one embodiment, the alkyl group has 1- 12 carbons designated here as Ci -Ci2-alkyl. In another embodiment, the alkyl group has 1-6 carbons designated here as C i-C6-alkyl. In another embodiment, the alkyl group has 1-4 carbons designated here as C1 -C4 alkyl. The alkyl group may be unsubstituted or substituted by one or more groups selected from halogen, hydroxyl, alkoxy carbonyl,

amido, alkylamido, dialkylamido, nitro, amino, alkylamino, dialkylamino, carboxyl, thio and thioalkyl. Each possibility represents a separate embodiment of the present invention.

An "aryl" group refers to an aromatic ring system containing from 6-14 ring carbon atoms. The aryl ring can be a monocyclic, bicyclic, tricyclic and the like. Non-limiting examples of aryl groups are phenyl, naphthyl including 1 -naphthyl and 2-naphthyl, and the like. The aryl group can be unsubstituted or substituted through available carbon atoms with one or more groups defined hereinabove for alkyl. Each possibility represents a separate embodiment of the present invention.

An "alkylaryl" group is an alkyl group as defined herein bonded to an aryl group as defined herein. The aryl group can be unsubstituted or substituted through available carbon atoms with one or more groups defined hereinabove for alkyl.

"Halide" or "halo" refers to F, CI, Br, or I.

The term "hydroxyl protecting groups" refers to protecting groups suitable for the protection of the hydroxyl moiety, which may also be referred to as "protected hydroxyl group". Such groups are known and exemplified in the art, such as in Greene's Protective Groups on Organic Synthesis 4th Edition, John Wiley & Son, Peter G. M. Wuts, Theodora W. Greene, Print ISBN: 9780471697541. Preferred protecting groups are trityl, benzyl, naphthyl, p-methoxybenzyl, p-nitrobenzyl, benzoyl, a substituted benzoyl, acetyl, a substituted acetyl, pivaloyl, trimethylsilyl, triethylsilyl, isopropyldimethylsilyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl, thexyldimethylsilyl, allyl, methoxymethyl, (2-methoxyethoxy)methyl, tetrahydropyranyl.

"Silyl protecting group" is a protecting group wherein the oxygen is linked with a silicon atom. Silyl protecting groups are disclosed in the textbook mentioned above. Preferred silyl protecting groups are trimethylsilyl, triethylsilyl, isopropyldimethylsilyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl, thexyldimethylsilyl.

A "silylating agent" may be any reagent which is able to insert a silyl protecting group to a free hydroxyl. Silyl protecting groups are defined above.

The term "amine protecting group" refers to protecting groups suitable for the protection of the amine moiety, which may also be referred to as "protected amine group". Such groups are known and exemplified in the art, such as in Greene's Protective Groups on Organic Synthesis 4th Edition, John Wiley & Son, Peter G. M. Wuts, Theodora W. Greene, Print ISBN: 9780471697541. Preferred amine protecting groups are carbamates, amides, N-alkyl, N-aryl, N-silyl amino and N-sulfonyl derivatives. More preferred amine protecting groups are tert-butoxycarbonyl (Boc), benzyloxycarbonyl (Cbz), allyloxycarbonyl (Alloc), ethyloxycarbonyl, 9-fluorenylmethoxycarbonyl (Fmoc), 2,2,2-trichloroethyl carbonate (Troc), formyl, acetyl, trifluoroacetyl, benzoyl (Bz), benzyl (Bn), allyl (All), trityl (Tr), trimethoxyphenylmethylene, trimethylsilyl (TMS), tert-butyldimethylsilyl, (TBS), p-methoxybenzyl (PMB), p-halo-benzyl, diphenylmethyl, naphthylmethyl, benzenesulfonate, tosylate.

"Base" when used herein includes hydroxides or alkoxides, hydrides, or compounds such as amine and its derivatives, that accept protons in water or solvent. Thus, exemplary bases include, but are not limited to, alkali or alkaline earth metal hydroxides and alkoxides (i.e., MOR, wherein M is an alkali metal such as potassium, lithium, or sodium, and R is hydrogen or alkyl, as defined above, more preferably where R is straight or branched chain CI -5 alkyl, thus including, without limitation, potassium hydroxide, potassium tert-butoxide, potassium tert-pentoxide, sodium hydroxide, sodium, potassium or magnesium methoxide, sodium tert-butoxide, lithium hydroxide, magnesium hydroxide, calcium hydroxide, barium hydroxide); alkali metal hydrides (i.e., MH, wherein M is as defined above, thus including, without limitation, sodium, potassium, and lithium hydrides); alkylated disilazanes, such as, for example, potassium hexamethyldisilazane and lithium hexamethyldisilazane; carbonates such as potassium carbonate (K2CO3), sodium carbonate (Na2C03), potassium bicarbonate (KHCO3), and sodium bicarbonate (NaHC03), alkyl ammonium hydroxides such as tetrabutyl ammonium hydroxide (TBAH) and so forth. The term "base" may also refer amines and their derivatives, for example, trialkyl amine (e.g., Et3N, diisopropylethyl amine, etc.), aromatic amine (e.g., Ph-NH2, PhN(Me)H, etc.) or other heterocyclic amines (morpholine, N-methyl-morpholine, etc); alkali metal alkoxides; alkali metal hydrides; alkylated disilazides; and non-aqueous carbonates.

The term "acid" might refer to the following: strong acids such as mineral acids, e. g. sulphuric acid, phosphoric acid, boric acid, nitric acid or hydrohalic acids; strong organic carboxylic acids, such as alkanecarboxylic acids of 1 to 4 carbon atoms which are unsubstituted or substituted (e. g., by halogen), such as acetic acid and trifluoroacetic acid; saturated or unsaturated dicarboxylic acids, for example oxalic, malonic, succinic, maleic, fumaric, phthalic or tetraphthalic; hydroxycarboxylic acids, for example ascorbic, glycolic, lactic, malic, tartaric or citric acid; aminoacids, for example aspartic or glutamic acid; benzoic acid; organic sulfonic acids, such as (Cl-4)-alkyl- or aryl-sulfonic acids which are substituted or unsubstituted (for example, by a halogen) such as methane-, trifluoromethane or p-toluene sulfonic acid.

The term "acid" might further refer to heteropolyacids. Heteropolyacids are the acidic forms of polyoxometalates. They usually contain a metal, such as tungsten, molybdenum or vanadium, oxygen, a hetero atom (an element of the p-block of the periodic table, such as silicon phosphorus or arsenic) and acidic hydrogen atoms. A more comprehensive definition is provided in "Enviromentally Bening Catalysts: For Clean Organic Reactions" , Part 3 (Transition Metal- Substituted Salt of Tungsten-Based Polyoxometalate-Supported Mesoporous Silica as a Catalyst for Organic Transformation Reactions, Chapter I , Springer Publications, ISBN 978-94-007-6709-6. Examples of heteropolyacids are phosphotungstic acid (H3PW12O40), phoshpomolybdic acid (H3PM012O40) and silicotungstic acid (H4S1W12O40), silicomolybdic acid (H4S1M012O40).

Acceptable salts of the compounds prepared herein include suitable acid addition salts thereof. Salts are formed, for example, with strong acids such as mineral acids, e. g. sulphuric acid, phosphoric acid, nitric acid, boric acid or hydrohalic acids; with strong organic carboxylic acids, such as alkanecarboxylic acids of 1 to 4 carbon atoms which are unsubstituted or substituted (e. g., by halogen), such as acetic acid and trifluoroacetic acid; with saturated or unsaturated dicarboxylic acids, for example oxalic, malonic, succinic, maleic, fumaric, phthalic or tetraphthalic; with hydroxycarboxylic acids, for example ascorbic, glycolic, lactic, malic, tartaric or citric acid; with aminoacids, for example aspartic or glutamic acid; with benzoic acid; or with organic sulfonic acids, such as (Cl-4)-alkyl- or aryl-sulfonic acids which are substituted or unsubstituted (for example, by a halogen) such as methane- or p-toluene sulfonic acid.

Pharmaceutically acceptable salts are salts that retain the desired biological activity of the parent compound and do not impart undesired toxicological effects. Examples of such salts are (a) acid addition salts formed with inorganic acids, for example hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid and the like; and salts formed with organic acids such as, for example, acetic acid, oxalic acid, tartaric acid, succinic acid, maleic acid, fumaric acid, gluconic acid, citric acid, malic acid, ascorbic acid, benzoic acid, tannic acid, palmitic acid, alginic acid, polyglutamic acid, naphthalenesulfonic acid, methanesulfonic acid, p-toluenesulfonic acid, naphthalenedisul fonic acid, polygalacturonic acid, and the like; (b) salts formed from elemental anions such as chlorine, bromine, and iodine, and (c) salts derived from bases, such as ammonium salts, alkali metal salts such as those of sodium and potassium, alkaline earth metal salts such as those of calcium and magnesium, and salts with organic bases such as dicyclohexylamine and N-methyl-D-glucamine. A review of suitable pharmaceutical salts may be found in Berge et al., J. Pharm. Sci., 66, 1, 19 (1977).

Additionally, it should be understood in the methods of preparation and claims herein, that the pronoun "a", when used to refer to a reagent, such as "a base", "a solvent" and so forth, is intended to mean "at least one" and thus, include, where suitable, single reagents as well as mixtures of reagents.

DETAILED DESCRIPTION OF THE INVENTION

The present invention discloses novel methods for the production of intermediates used in the preparation of Raltegravir.

According to a first embodiment of the present invention, there is provided a process for the preparation of compound of formula II,

II

wherein R represents hydrogen or an amine protecting group and R' represents hydrogen or a hydroxyl protecting group, comprising:

a) treating compound of formula III, wherein R" represents hydrogen, alkyl, aryl or aralkyl group, with a halomethylhalosilane reagent S, of general formula HalCH2Si(Hal)A2, wherein each Hal, independent of one another, represents a halogen atom and A represents alkyl, aryl or aralkyl;

III

b) treating the resulting intermediate product 1 with p-fluorobenzylamine;

c) treating the resulting intermediate product 2 with a fluoride ion source to provide compound of formula II.

Step a comprises the formation of intermediate product 1, upon reaction of compound of formula III with a halomethylhalosilane reagent (S). It has been found that the use of such reagents, instead of more conventional methylating agents used in prior art, results in the selective preparation of compound of formula II, against O-methylated isomers, with high overall yield.

In a preferred embodiment both halogens of the S reagent are chloro atoms. In a more preferred embodiment reagent S is (chloromethyl)dimethylchlorosilane ClCH2SiMe2Cl (S-l).

Typically 1.0-3.0 equivalents of the S reagent are used. Preferable are 1.0-2.0 equivalents. More preferable are 1.0-1.5 equivalents.

The reaction may also comprise the use of an activating agent. This agent activates the nitrogen atom of the - NH - moiety of the heterocyclic ring, to facilitate the reaction with the S reagent. Preferable activating agents are silylating agents. Preferable silylating agents are trialkylsilyl halides, bis(trimethylsilyl)acetamide, tetrachlorosilane, hexamethyldisilazane, diphenylmethylchlorosilane, t-

butyldimethylchlorosilane. Even more preferable is hexamethyldisilazane. More preferable are trimethylsilylchloride, hexamethyldisilazane.

The reaction is typically performed in polar organic solvents. Preferable are aprotic polar organic solvents.

The temperature of the reaction may vary between room temperature and 200 °C. Preferable is a range between room temperature and 100 °C. More preferable is a range between room temperature and 80 °C.

Step b comprises the formation of the amide bond with p-fluorobenzylamine, to provide intermediate product 2. The reaction may be performed with 1.0-3.0 equivalents of p-fluorobenzylamine. In a preferred embodiment the equivalents used are 1.0-1.5. In a more preferred embodiment the equivalents used are 1.2-1.3.

The reaction may be performed in a polar organic solvent. Preferable are polar protic organic solvents. More preferable are alcohols.

The temperature may vary between room temperature and 200 °C. Preferable is a range between room temperature and 150 °C.

The reaction is performed preferably in the presence of a base. The base may be an alkali metal or alkaline earth hydroxide or alkoxide, carbonates, alkyl ammonium hydroxides, amines and their derivatives. Preferable are amines and their derivatives.

Step c comprises the cleavage of a silyl moiety from intermediate product 2, thereby providing compound II. Reagents suitable for this conversion are those which can behave as fluoride ion sources. Preferable are fluoride salts of metals or of tertiary amines. More preferable are fluoride salts of alkali metals and of tertiary amines. Even more preferable is potassium fluoride.

The reaction may be performed in a polar organic solvent. More preferable are alcohlols, ethers and esters. Even more preferable are methanol, ethanol, ethyl acetate and tetrahydrofuran.

In preferred embodiments, any of steps a, b, c or all of the steps a, b, c may be performed without isolation of intermediates. In a more preferred embodiment steps a, b, c are all performed without isolation of intermediates. This is beneficial,

particularly for industrial purposes, because the elimination of isolation of intermediates contributes to a higher overall yield and at the same time provides for a faster and simpler process.

According to a second embodiment of the present invention, there is provided a process for the preparation of Raltegravir or a pharmaceutically acceptable salt thereof, comprising steps a-c as described above and further comprising converting compound of formula II to Raltegravir or a pharmaceutically acceptable salt thereof. The conversion of compound II to Raltegravir is disclosed in prior art. By way of example, compound of formula II can be converted to Raltegravir by the process disclosed in WO2006060730 or WO2009088729.

According to a third embodiment of the present invention, there is provided a process for the preparation of compound of formula IV,

IV

wherein R' and R" are defined as above, comprising:

a) treating compound of formula III, as defined above, with a halomethylhalosilane reagent S, as defined above;

d) when R is other than hydrogen, removing protecting group R from resulting intermediate product 1;

e) treating resulting intermediate product 3 with compound of formula V, wherein Y represents halogen atom, or hydroxyl group, or alkoxide moiety - 0-X+, X being an alkali metal or alkaline earth metal;

f) treating resulting intermediate product 4 with a fluoride ion source to provide compound of formula IV.

Step a may be performed as described above.

Step d is a deprotection reaction which provides intermediate product 3 and may be performed in accordance with the nature of the protecting group. Standard deprotection reagents and conditions are illustrated in Greene's Protective Groups on Organic Synthesis 4th Edition, John Wiley & Son, Peter G. M. Wuts, Theodora W. Greene, Print ISBN: 9780471697541.

Step e comprises the formation of the amide bond with the oxadiazole moiety, to provide intermediate product 4. The reaction may be performed with 1.0-3.0 equivalents of compound of formula V.

The reaction may be performed in a polar organic solvent. Preferable are polar aprotic organic solvents.

The temperature may vary between room temperature and 200 °C. Preferable is a range between room temperature and 150 °C.

The reaction is performed preferably in the presence of a base. The base may be an an alkali metal or alkaline earth hydroxide or alkoxide, carbonates, alkyl ammonium hydroxides, amines and their derivatives. Preferable are amines and their derivatives.

Step f comprises the cleavage of a silyl moiety from intermediate product 4, thereby providing compound IV. It may be performed as disclosed for step c.

In preferred embodiments, any of steps a, d,e,f or all of the steps a, d, e, f may be performed without isolation of intermediates. In a more preferred embodiment steps a, d, e, f are all performed without isolation of intermediates. As disclosed above, this is beneficial.

According to a fourth embodiment of the present invention, there is provided a process for the preparation of Raltegravir or a pharmaceutically acceptable salt thereof, comprising steps a and d-f as described above and further comprising converting compound of formula IV to Raltegravir. The conversion of compound IV to Raltegravir is disclosed in prior art. By way of example, compound of formula IV can be converted to Raltegravir by the method disclosed in WO2013098854.

According to a fifth embodiment of the present invention, there is provided a process for the preparation of compounds of formulae II, IV, or salts thereof, wherein a methyl group is introduced selectively on the -NH- moiety of the heterocyclic ring, by a method which comprises: 1) reaction with a halomethylhalosilane reagent S, as defined above and 2) reaction with a fluoride ion source, optionally comprising further steps between them. According to this embodiment, step 1 comprises a reaction with the halomethylhalosilane reagent S, which is defined as above. In a preferred embodiment both halogens of the S reagent are chloro atoms. In a more preferred embodiment reagent S is CICHiSiMeiCl (S-l).

The reaction may also comprise the use of an activating agent. This agent activates the nitrogen atom of the - NH - moiety of the heterocyclic ring, to facilitate the reaction with the S reagent. Preferable activating agents are silylating agents. Preferable silylating agents are trialkylsilyl halides, bis(trimethylsilyl)acetamide, tetrachlorosilane, hexamethyldisilazane, diphenylmethylchlorosilane, t-butyldimethylchlorosilane. Even more preferable is hexamethyldisilazane. More preferable are trimethylsilylchloride, hexamethyldisilazane.

The reaction of step 2 comprises the cleavage of a silyl moiety. Reagents suitable for this conversion are those which can behave as fluoride ion sources. Preferable are fluoride salts of metals or of tertiary amines. More preferable are fluoride salts of alkali metals and of tertiary amines. Even more preferable is potassium fluoride.

The reaction may be performed in a polar organic solvent. More preferable are alcohlols, ethers and esters. Even more preferable are methanol, ethanol, ethyl acetate and tetrahydrofuran.

Advantageously, this method provides selective methylation on the -NH- moiety of the heterocyclic ring against the neighboring oxygen atom. The method may be performed irrespectively of any further steps interposed between steps 1 and 2. Hence the present method may be performed with or without further steps between steps 1 and 2.

According to a sixth embodiment of the present invention, there is provided a process for the preparation of Raltegravir or pharmaceutically acceptable salts thereof, wherein a methyl group is introduced selectively on the -NH- moiety of the

heterocyclic ring, by a method which comprises: 1) reaction with a halomethylhalosilane reagent S and 2) reaction with a fluoride ion source, optionally comprising further steps between them. Reactions of steps 1 and 2 may be performed as described above. The method may be performed irrespectively of any further steps interposed between steps 1 and 2. Hence the present method may be performed with or without further steps between steps 1 and 2.

According to a seventh embodiment of the present invention, there is provided a process for the preparation of compound of formula VI, wherein R, R' and R" are defined as above, comprising: a) treating compound of formula III, wherein R, R' and R" are defined as above, with a halomethylhalosilane reagent S, of general formula HalCH2Si(Hal)A2, wherein Hal and A are defined as above and g) treating the resulting intermediate product 1 with a fluoride ion source to provide compound of formula VI.

III VI

Step a) may be performed as described above. Step g) may be performed in accordance with step c).

According to an eighth embodiment of the present invention, there is provided the use of a halomethylhalosilane reagent S for the preparation of compounds of formulae II, IV, VI or salts thereof, or Raltegravir or pharmaceutically acceptable salts thereof, through a method of selective introduction of a methyl group on the -NH- moiety of the heterocyclic ring. The halomethylhalosilane reagent S may be used as set forth above.

Within the scope of the present invention, each of the intermediate products 1-4 may comprise a single compound or mixtures of compounds. The chemical profile of those intermediate products depends on the choice of reagent S, the exact structure of the compounds used as starting materials, compounds of formulae III and IV, and the reaction conditions of steps a-f. These factors may influence the multifunctional character of reagent S, resulting in more or less complex chemical structures. This, however, does not jeopardize the selective character of the methylation method of the present invention.

In a preferred embodiment, intermediate product 1 comprises a compound of formula 1A, wherein R, R' and R" are as defined above. In a more preferred embodiment, intermediate product 1 is represented by compound of formula 1A .

1 A

In a preferred embodiment, intermediate product 1 comprises a compound of formula 1B, wherein R, R' and R" are as defined above. In a more preferred embodiment, intermediate product 1 is represented by compound of formula 1B .

CI

1 B

In a preferred embodiment, intermediate product 2 comprises a compound of formula 2A, wherein R and R' are as defined above. In a more preferred embodiment, intermediate product 2 is represented by compound of formula 2A .

2 A

In a preferred embodiment, intermediate product 2 comprises a compound of formula 2B, wherein R and R' are as defined above. In a more preferred embodiment, intermediate product 2 is represented by compound of formula 2B .

In a preferred embodiment, intermediate product 3 comprises a compound of formula 3A, wherein R' and R" are as defined above. In a more preferred embodiment, intermediate product 3 is represented by compound of formula 3A .

In a preferred embodiment, intermediate product 3 comprises a compound of formula 3B, wherein R' and R" are as defined above. In a more preferred embodiment, intermediate product 3 is represented by compound of formula 3B -

In a preferred embodiment, intermediate product 4 comprises a compound of formula 4A, wherein R' and R" are as defined above. In a more preferred embodiment, intermediate product 4 is represented by compound of formula 4A .

In a preferred embodiment, intermediate product 4 comprises a compound of formula 4B, wherein R' and R" are as defined above. In a more preferred embodiment, intermediate product 4 is represented by compound of formula 4B .

CI

4 B

According to a ninth embodiment of the present invention, there is provided a process for the preparation of intermediate product 1, comprising:

a) treating compound of formula III, as defined above, with a halomethylsilyl reagent S, as defined above;

g) isolating intermediate product 1.

According to a tenth embodiment of the present invention, there is provided intermediate product 1, obtainable by a process as described above.

According to a eleventh embodiment of the present invention, there are provided compounds of formulae 1A and 1B .

According to an twelfth embodiment of the present invention, there is provided a process for the preparation of intermediate product 2, comprising:

a) treating compound of formula III, as defined above, with a halomethylsilyl reagent S, as defined above;

b) treating the resulting intermediate product 1 with p-fluorobenzylamine;

h) isolating intermediate product 2.

According to a thirteenth embodiment of the present invention, there is provided intermediate product 2, obtainable by a process as described above.

According to a fourteenth embodiment of the present invention, there are provided compounds of formulae 2A and 2B .

According to a fifteenth embodiment of the present invention, there is provided the use of intermediate products 1, 2 or compounds of formulae 1A, 1B , 2A or 2B for the preparation of compound of formula II.

According to a sixteenth embodiment of the present invention, there is provided a process for the preparation of intermediate product 3, comprising:

a) treating compound of formula III, as defined above, with a halomethylsilyl reagent S, as defined above;

d) when R is other than hydrogen, removing protecting group R from resulting intermediate product 1;

i) isolating intermediate product 3.

According to a seventeenth embodiment of the present invention, there is provided intermediate product 3, obtainable by a process as described above.

According to a eighteenth embodiment of the present invention, there are provided compounds of formulae 3A and 3B .

According to an nineteenth embodiment of the present invention, there is provided a process for the preparation of intermediate product 4, comprising:

a) treating compound of formula III, as defined above, with a halomethylsilyl reagent S, as defined above;

d) when R is other than hydrogen, removing protecting group R from resulting intermediate product 1;

e) treating resulting intermediate product 3 with compound of formula V, as defined above;

j) isolating intermediate product 4.

According to a twentieth embodiment of the present invention, there is provided intermediate product 4, obtainable by a process as described above.

According to a twenty-first embodiment of the present invention, there are provided compounds of formulae 4A and 4B .

According to a twenty-second embodiment of the present invention, there is provided the use of intermediate products 3, 4 or compounds of formulae 3A , 3B , 4A or 4B for the preparation of compound of formula IV.

According to a twenty-third embodiment of the present invention, there is provided the use of intermediate products 1, 2, 3 or 4 or compounds of formulae 1A , 1B , 2A , 2B , 3A, 3B , A or 4B for the preparation of Raltegravir or pharmaceutically acceptable salts thereof.

According to a twenty-fourth embodiment of the present invention, there is provided the use of intermediate product 1 or compounds of formulae 1A , 1B for the preparation of compound of formula VI.

The present invention further provides an improved process for the effective acylation of amine intermediate compound of formula lib with oxadiazole intermediate compound of formula V.

According to a twenty-fifth embodiment of the present invention, there is provided a process for the preparation of Raltegravir or a pharmaceutically acceptable salt thereof, comprising acylation of amine intermediate compound of formula lib with oxadiazole intermediate compound of formula V in the presence of a silylating agent and an acid.

v

Oxadiazole intermediate compound of formula V may be a carboxylic acid, a carboxylic acid halide, or a carboxylic acid salt of alkali metal or alkaline metal earth, as defined above.

The silylating agent may be used as a dehydrating agent and/or as temporary protection of the phenolic group to facilitate the acylation reaction to the amine. The inventors have found that the silylating agent might actually hinder the reaction, rather than enhancing it, by participating in a side reaction with oxadiazole intermediate compound of formula V.

The present invention solves this problem by performing the acylation reaction in the presence of an acid, along with the silylating agent.

The acid may be a mineral acid, a strong organic carboxylic acid, saturated or unsaturated dicarboxylic acid, hydroxycarboxylic acid, benzoic acid or an organic sulfonic acid as defined above. The acid may also be a heteropolyacid, a zeolite or an ion-exchange resin.

Preferable acids are mineral acids, strong organic carboxylic acids, organic sulfonic acids and heteropolyacids. More preferable are sulphuric acid, nitric acid, phosphoric acid, hydrohalic acids, boric acid, formic acid, acetic acid and its halo-substituted derivatives, propionic acid, butanoic acid, methanesulfonic acid, trifluoromethanesulfonic acid, p-toluenesulfonic acid, phosphotungstic acid (H3PW12O40), phoshpomolybdic acid (H3PM012O40), silicotungstic acid (H4S1W12O40) and silicomolybdic acid (H4S1M012O40). Even more preferable are sulphuric acid, nitric acid, phosphoric acid, hydrohalic acids, boric acid, formic acid, acetic acid, trifluoroacetic acid, methanesulfonic acid, trifluoromethanesulfonic acid and p-toluenesulfonic acid.

The acid may be used in a catalytic amount. Preferably the equivalents are 0.2-0.005 per mole of compound of formula lib. More preferably the equivalents are 0.1-0.01 per mole of compound of formula lib.

The silylating agent may be any reagent which is able to insert a silyl protecting group to a free hydroxyl and has been defined above. Preferable silylating agents are trimethylsilyl, triethylsilyl, isopropyldimethylsilyl, tert-butyldimethylsilyl, tert-butyldiphenylsilyl and thexyldimethylsilyl halides, bis(trimethylsilyl)acetamide, tetrachlorosilane, hexamethyldisilazane. More preferable are trimethylsilyl chloride and hexamethyldisilazane. Even more preferable is hexamethyldisilazane.

The equivalents of the silylating agent depend on the nature of the silylating agent, as some of them are able to silylate two hydroxyl groups per molecule of silylating agent. The equivalents of the silylating agent may therefore be 0.4-2.0 per mole of compound of formula lib. More preferably the equivalents are 0.6-1.4 per mole of compound of formula lib.

The reaction may be performed in an organic solvent or water or mixtures thereof. Preferable organic solvents are ethers, nitriles, halogenated or non-halogenated aliphatic and aromatic hydrocarbons and mixtures thereof.

The equivalents of oxadiazole intermediate compound of formula V may be 1.0 to 2.0 per mole of compound of formula lib. Preferably the equivalents are 1.2 to 1.7 per mole of compound of formula lib. More preferably the equivalents are 1.3 to 1.6 per mole of compound of formula lib.

The oxadiazole intermediate compound of formula V may be used in the reaction in the form of a carboxylic acid halide, which may be prepared shortly before the acylation reaction. Standard methods for this transformation are well known to the skilled person and include reaction with a reagent which converts the carboxylic acid or its salt to its respective halide. Such reagents are oxalyl chloride, thionyl chloride, phosphorous (III) and (V) chloride, phosphorus oxychloride. Preferable is oxalyl chloride.

The acylation reaction may also comprise the presence of a base. Prefarable bases are non-aqueous bases. More preferable are amines and their derivatives. Even more preferable are morpholine, N-methyl morpholine, trimethylamine, diisopropylethylamine.

Compound of formula lib may be prepared by deprotection of a suitably protected precursor (lie), as described for example in WO2006060730.

lie Mb

EXAMPLES

EXAMPLE 1

Via

A 500 ml 3-necked round bottom flask, equipped with a reflux condenser and a thermometer is charged with 15.0 g (41.5 mmol) methyl 2-(2(((benzyloxy)carbonyl)amino)propan-2-yl)-5,6-dihydroxy-l,6dihydropyrimidine-4-carboxylate Ilia and 150 ml acetonitrile under inert atmosphere at 20-30 °C. To the resulting suspension is added under stirring 8.7 ml hexamethyldisilazane (41.5 mmol; 1.0 equiv) and mixture is heated up at 80 °C for 1 h. While still hot 6.0 ml (chloromethyl)-dimethylchlorosilane (45.7 mmol, 1.10 equivs) is added and heating is maintained for 2 h. Reaction progress is monitored by TLC. Upon completion the volatiles are evaporated off and the residue is further dried under high vacuum for at least 30 min. To the residue is added 75 ml ethyl acetate and mixture is heated up and stirred until a clear solution arises. 3.0 g potassium fluoride (51.9 mmol; 1.25 equivs) is added and stirring is maintained for approximately lh. Upon completion, 50 ml demineralized water is added. After stirring for 10 min, the mixture is left to settle. The upper organic phase is collected and washed with 50 ml water, then dried over anhydrous sodium sulphate, filtered and evaporated down to afford Via as off-white solid (12.1 g; 78% yield; >98% purity).

EXAMPLE 2

I said Ilia

In a round-bottomed flask 1.0 g of methyl 2-(2-(benzyloxycarbonylamino)propan-2-yl)-5-hydroxy-6-oxo-l,6-dihydropyrimidine-4-carboxylate (compound of formula Ilia) and 16mL Acetonitrile are charged at 20-25 °C and the mixture is stirred for 5 min. To the suspension 0.58 mL Hexamethyldisilazane is added. The solution is heated to reflux temperature (80 °C) and it is stirred at that temperature for lhour. Then, 0.4 mL of Chloro(chloromethyl)dimethylsilane is added over 5min. at reflux temperature. The mixture is stirred for another 4 hours at reflux temperature (80°C). Then the mixture is cooled to 20-25 °C and filtered under vacuum. The filtered solid is rinsed with 5mL Acetonitrile. The filtrates are evaporated to give 1.4 g of crude intermediate product 1.

To the above mentioned product, 5mL of Methanol are added till complete dissolution. To this solution, 0.46mL of Triethylamine and 0.38mL of 4-Fluorobenzylamine are added. The solution is heated to reflux temperature (65 °C) and stirred at that temperature for 2 hours. Then, the mixture is cooled to 20-25°C. The solution is concentrated to give 1.96 g of crude intermediate product 2.

To the above mentioned product 8mL of Tetrahydrofuran and 0.5 g of Cesium Fluoride are added and the mixture is heated to reflux temperature (66°C). The mixture is stirred at that temperature for 45min. and then cooled to 20-25°C. Another lOmL Tetrahydrofuran is added and the mixture is stirred at 20-25°C for 30min. The mixture is filtered under vacuum and the solid is rinsed with 5mL Tetrahydrofuran and suck-dried to give 1.38g off-white solid (compound of formula Ila).

EXAMPLE 3

A 2 L 3-necked round bottom flask, equipped with a mechanical stirrer, a reflux condenser and a thermometer is charged with 150.0 g (0.42 mol) methyl 2-(2(((benzyloxy)carbonyl)amino)propan-2-yl)-5,6-dihydroxy-l,6dihydropyrimidine-4- carboxylate Ilia and 0.75 L acetonitrile under inert atmosphere at 20-30 °C. To the resulting suspension is added under stirring 87 ml hexamethyldisilazane (0.42 mol; 1.0 equiv) and mixture is heated up at 80 °C for 1 h. While still hot 60.4 ml (chloromethyl)- dimethylchlorosilane is added over 10-15 min and heating is maintained for 2 h. Reaction progress is monitored by TLC. Upon completion the reaction is evaporated down and the residue is further dried under high vacuum for at least 30 min. To the residue is added 0.75 L methanol and mixture is stirred until a clear solution arises. 69.7 ml (0.50 mol; 1.20 equivs) triethylamine is added followed by 57.2 ml (0.50 mol; 1.20 equivs) 4-fluoro-benzylamine and the resulting mixture is heated up to 65 °C for 1 h. While still hot potassium fluoride (0.53 mol; 1.25 equivs) is added in one portion and heating maintained for 1-2 h. Upon completion of the desilylation, a colorless solid is separated from the reaction mixture. Reaction is cooled down to ambient temperature and 1.12 L demineralized water is added and slurry is stirred for 1 h. Crude Benzyl (2-(4-((4-fluorobenzyl)carbamoyl)-5-hydroxy-l-methyl-6-oxo-l,6-dihydropyrimidin-2-yl)propan-2-yl)carbamate Ila is afforded by filtration under vacuum. The wet cake is washed with 0.45 L methanol/water (1:2) and suck dried for 1 h. The colorless solid is collected and dried further under vacuum at 30 °C for 10 h. After recrystallization with methanol/water = 3: 1, pure Ila is obtained as colorless solid (126.8 g, 82%).

EXAMPLE 4

Ila Mb

A 1L r.b. flask equipped with a magnetic bar is charged with 3.6 g (7.6 mmol) compound of formula Ila, followed by 60 ml methanol. Add 0.5 ml (8.4 mmol) glycolic acid in one portion under argon atmosphere. Add 0.72 g 5% Pd/C (50% w/w) and degas reaction mass under Argon atmosphere. Reaction mass is hydrogenated in atmospheric pressure for 10 h. The reaction mass is filtered and catalyst is washed with 30 ml methanol. The combined filtrate is concentrated to a total volume of 35 ml. Add 1.27 ml (9.1 mmol) triethylamine dropwise over a period of 30 min. Stir the resulting slurry at 0-5 °C for lh. Filter solid and wash with 5 ml methanol. Solid is dried under vacuum to afford 1.95 g of compound of formula lib.

EXAMPLE 5

Mb

A r.b. flask (flask A) is charged with 5.0 g (14.95 mmol) of compound of formula lib followed by 40 ml dichloromethane. To the resulting suspension is added 11.6 ml trimethylsilyl chloride (91.64 mmol, 6.13 eq.) and the mixture is heated up to 45 °C for lh. The reaction mass is cooled down to -10 °C and 4.8 ml N-methylmorpholine (43.8 mmol, 2.93 eq.) is added dropwise over 10 minutes and the resulting mixture is stirred further for lh at the same temperature. Another r.b. flask (flask B) is charged with 3.73 g (22.43 mmol, 1.50 eq.) potassium 5-methyl-l,3,4-oxadiazole-2-carboxylate Va (potassium salt of compound of formula V) followed by 40 ml dichloromethane and mixture is cooled down to -10 °C. 0.06 ml dimethylformamide (0.75 mmol, 0.05 eq.) is added followed by 1.93 ml oxalyl chloride (22.43 mmol, 1.50 eq.). Stirring is maintained for 45-60 minutes before the slurry of flask A is transferred to flask B over 15 minutes at -10 °C. The resulting mixture is stirred further for lh. 100 ml demineralized water is added slowly to the reaction mass and the latter is left to reach ambient temperature and stirred there for 15 minutes. The resulting mixture is filtered through a celite bed and the wet cake is washed with 20 ml dichloromethane. The filtrate is left to settle and the organic phase is collected and evaporated down under vacuum at 40 °C. 5.16 g of compound of formula I is obtained.

EXAMPLE 6

A slurry suspension of 100 g (0.213 mol) Benzyl (2-(4-((4-fluorobenzyl)carbamoyl)-5-hydroxy-l-methyl-6-oxo-l,6-dihydropyrimidin-2-yl)propan-2yl)carbamate IVa, 38 ml (0.426 mol) glycolic acid (67% w/w) and 5g 5% Pd/C (50% w/w) in 3L methanol was hydrogenated at 130 psi for 3-4 h (>99.6% purity). The reaction mixture was filtered through celite bed and washed with 100 ml methanol. The combined filtrate was concentrated to a total volume of 1.1L at 25-28 °C and neutralized by 65 ml triethylamine to pH= 9-10 at 25-28 °C. The formed crystalline solid is collected by filtration, rinsed with 100ml methanol and dried under vacuum to afford 65 g (0.194 mol) of free amine (lib).

1H-NMR (500 MHz, DMSO-d6) δ 10.16 (br, 1 H), 7.33-7.30 (m, 2H), 7.14-7.10 (m, 2 H), 4.45 (d, J = 6.0 Hz, 2 H), 3.68 (s, 3 H), 1.56 (s, 6 H).

13C-NMR (125 MHz, DMSO-d6) δ 168.1, 162.1, 160.2, 135.6, 129.3, 123.2, 115.0, 55.9, 48.6, 41.3, 33.1, 28.2.

EXAMPLE 7

A mixture of oxadiazole K-salt Va (79.5 g, 0.478 mol), acetonitrile (400 ml), and DMF (2.47 ml) was cooled to -5 °C and oxalyl chloride (38.4 ml, 0.448 mol) is added over a course of 30 min. The resulting slurry was aged at 0-5 °C for lh, then cooled to -10 °C.

A slurry of amine lib (100 g, 0.299 mol) and THF (1L) was stirred at room temperature and HMDS (49.5 ml, 0.239 mol) is added dropwise over a period of 5 min. To the resulting suspension 1.71g p-TsOH (8.9 mmol) were added in one portion. The reaction mixture was heated at 60-65 °C for 2h, then cooled down at -5- 0 °C and N-methylmorpholine (98.8 ml, 0.898 mol) was added. The resulting solution was aged at -5 °C for 20 min. The oxadiazole acid chloride Va was added dropwise to the amine containing slurry reaction mixture at -5 °C over a course of 45 min. Reaction is monitored with HPLC and after completion 20% aq. potassium hydroxide (500 ml) was added and the reaction mixture is stirred at 5 °C for lh. HC1 2N (370 ml) was added over 10 min to adjust pH at 3-4 and reaction mass is warmed at 15 °C. 3.75 L ml water were added slowly over a course of lh. The resulting white suspension was aged at 15 °C for 1 h and filtered. The cake was washed with 300 ml water/acetonitrile (2.5: 1) and 300 ml water. White solid is dried to afford Raltegravir

1 (115 g, 0.258 mol).

1H-NMR (500 MHz, DMSO-d6) δ 12.21 (s, 1 H), 9.86 (s, 1H), 9.08 (t, J = 6.4 Hz, 1 H), 7.41-7.38 (m, 2 H), 7.19-7.15 (m, 2 H), 4.52 (d, J = 6.4 Hz, 2 H), 3.49 (s, 3 H), 2.56 (s, 3 H), 1.74 (s, 6 H).

13C-NMR (125 MHz, DMSO-d6) δ 168.4, 165.7, 162.2, 160.3, 158.5, 158.1, 152.6, 151.8, 145.7, 134.9, 129.5, 124.4, 115.2, 57.6, 41.6, 32.9, 27.0, 10.7.

EXAMPLE 8

Raltegravir potassium salt

the

To a suspension of Raltegravir I (100 gr, 0.225 mol) in ethanol (1L) a solution of KOH (17.7 g) in 750 ml water was added slowly over a period of 30 min. To the slurry reaction mixture 1 L ethanol was added over a course of 30 min. The reaction mixture was stirred at 25 °C for 90 min. white solid was obtained by filtration and rinsed with 500 ml ethanol. The white solid was dried to afford Raltegravir K-salt la (95 g, 0.196 mol).

mp 267.2-267.6 ° C

1H-NMR (500 MHz, DMSO-d6) δ 11.68 (s, 1 H), 9.74 (s, 1H), 7.34-7.31 (m, 2 H), 7.13-7.10 (m, 2 H), 4.45 (d, J = 6.0 Hz, 2 H), 3.40 (s, 3 H), 2.56 (s, 3 H), 1.70 (s, 6 H).

13C-NMR (125 MHz, DMSO-d6) 5167.3, 165.9, 165.4, 161.9, 159.9, 158.6, 157.4, 151.9, 137.9, 137.1, 129.0, 122.4, 114.9, 57.2, 40.9, 32.1, 26.9, 10.7.

CLAIMS

1. A process for the preparation of compound of formula II, wherein R represents hydrogen or an amine protecting group and R' represents hydrogen or a hydroxyl protecting group, comprising:

II

a) treating compound of formula III, wherein R" represents hydrogen, alkyl, aryl or aralkyl group, with a halomethylhalosilane reagent S, of general formula HalCH2Si(Hal)A2, wherein each Hal, independent of one another, represents a halogen atom and A represents alkyl, aryl or aralkyl;

III

b) treating the resulting intermediate product 1 with p-fluorobenzylamine;

c) treating the resulting intermediate product 2 with a fluoride ion source to provide compound of formula II.

2. A process for the preparation of Raltegravir or a pharmaceutically acceptable salt thereof, comprising steps a-c as described in claim 1 and further comprising converting compound of formula II to Raltegravir or a pharmaceutically acceptable salt thereof.

A process for the preparation of compound of formula IV, wherein R' and R" defined in claim 1 , comprising:

IV

a) treating compound of formula III, as defined in claim 1 , with a halomethylhalosilane reagent S, as defined in claim 1 ;

d) when R is other than hydrogen, removing protecting group R from resulting intermediate product 1;

e) treating resulting intermediate product 3 with compound of formula V, wherein Y represents halogen atom, or hydroxyl group, or alkoxide moiety - 0"X+, X being an tal or alkaline earth metal;

f) treating resulting intermediate product 4 with a fluoride ion source to provide compound of formula IV.

4. A process for the preparation of Raltegravir or a pharmaceutically acceptable salt thereof, comprising steps a and d-f as described in claim 3 and further comprising converting compound of formula IV to Raltegravir or a pharmaceutically acceptable salt thereof.

5. A process for the preparation of compound of formula VI, wherein R, R' and R" are defined in claim 1, comprising: a) treating compound of formula III, wherein R, R' and R" are defined as in claim 1, with a halomethylhalosilane reagent S, of general formula HalCH2Si(Hal)A2, wherein Hal and A are defined as in claim 1 and g) treating the resulting intermediate product 1 with a fluoride ion source to provide compound of formula VI.

6. A process for the preparation of compounds of formulae II, IV, or salts thereof, wherein a methyl group is introduced selectively on the -NH- moiety of the heterocyclic ring, by a method which comprises: i) reaction with a halomethylhalosilane reagent S, as defined in claim 1 and ii) reaction with a fluoride ion source, optionally comprising further steps between them.

7. A process for the preparation of Raltegravir or pharmaceutically acceptable salts thereof, wherein a methyl group is introduced selectively on the -NH- moiety of the heterocyclic ring, by a method which comprises: i) reaction with a

halomethylhalosilane reagent S, as defined in claim 1 and ii) reaction with a fluoride ion source, optionally comprising further steps between them.

Use of a halomethylhalosilane reagent S for the preparation of compounds of formulae II, IV, VI or salts thereof or Raltegravir or pharmaceutically acceptable salts thereof, through a method of selective introduction of a methyl group on the - NH - moiety of the heterocyclic ring.

A process for the preparation of Raltegravir or a pharmaceutically acceptable salt thereof, comprising acylation of intermediate lib with oxadiazole intermediate compound of formula V in the presence of a silylating agent and an acid.

v

10. A process according to claim 9 wherein the silylating agent is selected from trimethylsilyl, triethylsilyl, isopropyldimethylsilyl, tert-butyldimethylsilyl, tert- butyldiphenylsilyl and thexyldimethylsilyl halides, bis(trimethylsilyl)acetamide, tetrachlorosilane , hexamethyldisilazane .

11. A process according to claim 10, wherein the silylating agent is hexamethyldisilazane.

12. A process according to any of the preceding claims, wherein the acid is selected from mineral acids, strong organic carboxylic acids, saturated or unsaturated dicarboxylic acids, hydroxycarboxylic acids, benzoic acid, organic sulfonic acids, heteropoly acids, zeolites or ion-exchange resin.

13. A process according to claim 12, wherein the acid is selected from mineral acids, strong organic carboxylic acids, organic sulfonic acids and heteropoly acids.

14. A process according to claim 13, wherein the acid is selected from sulphuric acid, nitric acid, phosphoric acid, hydrohalic acids, formic acid, acetic acid and its halo- substituted derivatives, propionic acid, butanoic acid, methanesulfonic acid, trifluoromethanesulfonic acid, p-toluenesulfonic acid, phosphotungstic acid, phoshpomolybdic acid, silicotungstic acid and silicomolybdic acid.

15. A process, according to any preceding claim, wherein the acid is present in a catalytic amount.

16. A process, according to claim 15, wherein the acid is present at 0.005-0.2 equivalents per mole of compound of formula lib.