PROCESS FOR THE PREPARATION OF 2-(CYCLOHEXYLMETHYL)-/V-{2-[(2S)-1-
METHYLPYRROLIDIN-2-YL]ETHYL}-1,2,3,4-TETRAHYDROISOQUINOLINE-7-
SULFONAMIDE
FIELD OF THE INVENTION
The present invention relates to processes for preparing 2-(cyclohexylmethyl)-/V-{2-
[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide,
various intermediates thereto and pharmaceutically acceptable salts thereof.
BACKGROUND OF THE INVENTION
The histamine H3 receptors are found in the central and peripheral nervous systems.
The administration of histamine H3 receptor ligands may influence the secretion of
neurotransmitters in the brain and the periphery and thus can be useful in the
treatment of several disorders, including Alzheimer's disease and other dementias,
obesity, central nervous system disorders such as vigilance and sleep disorders,
narcolepsy, Parkinson's disease, attention-deficit hyperactivity disorder, memory and
learning disorders, epilepsy, schizophrenia, moderate cognitive disorders,
depression, anxiety, cardiovascular disorders, and gastrointestinal disorders.
To illustrate, a number of studies in the literature have demonstrated the cognitive
enhancing properties of histamine H3 receptors antagonists in rodent models (See,
e.g., Giovanni et al., Behav. Brain Res., 1999, 104, 147-155). These reports further
suggest that antagonists and/or inverse agonists could be useful for the treatment of
cognitive impairments in neurological diseases such as Alzheimer's disease and
related neurodegenerative disorders. Alzheimer's disease is the most common
cause of dementia in the elderly, and is often characterized with one or more
symptoms such as memory loss, confusion, irritability and aggression, mood swings,
language breakdown, long-term memory loss, withdrawal of the sufferer, and loss of
motor control.
2-(Cyclohexylmethyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide, which has the structure of Formula (I):
is a potent histamine H3 receptor antagonist with inverse agonist properties. A
preparation and the physical properties and beneficial pharmacological properties of
2-(cyclohexylmethyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide are described in, for example, WO2005/1 8547
(also US2007/01 05834).
WO2005/1 18547 describes a general method of synthesis which is difficult to
transpose to the industrial scale for production in large quantities. This method of
synthesis entails reacting 2-(2,2,2-trifluoroacetyl)-1 ,2,3,4-tetrahydro-isoquinoline-7-
sulfonyl chloride with (+/-)-2-(2-aminoethyl)-1-methylpyrrolidine, which product is
deprotected in methanol and hydrochloric acid. The enantiomers are next separated
by chiral chromatography. The resulting W-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-
1,2,3,4-tetrahydroisoquinoline-7-sulfonamide undergoes reductive amination with
cyclohexanecarboxaldehyde in the presence of a palladium catalyst. 2-
(Cyclohexylmethyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide is isolated as the free base and converted to a
salt.
The present invention makes it possible to optimize the synthesis of 2-
(cyclohexylmethyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1, 2,3,4-
tetrahydroisoquinoline-7-sulfonamide for industrial use by avoiding the chiral
chromatographic separation of the enantiomers of (+/-)-A/-[2-(1-methylpyrrolidin-2-
yl)ethyl]-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide. The present invention deals
with the chirality issues first, allowing the coupling of 2-(2,2,2-trifluoroacetyl)-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonyl chloride with enantiomerically pure (>99% ee) (-)-2-
(2-aminoethyl)-1-methylpyrrolidine. In so doing, more of the 1,2,3,4-
tetrahydroisquinoline moiety in the starting sulfonyl chloride can be incorporated into
product. Using the above-described synthesis, half of this material would have been
discarded with the unwanted enantiomer of A/-[2-(1-methylpyrrolidin-2-yl)ethyl]-
1,2,3,4-tetrahydroisoquinoline-7-sulfonamide.
BRIEF SUMMARY OF THE INVENTION
Accordingly, the present invention provides a process for producing 2-
(cyclohexylmethyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide and salts thereof, of high purity and in a
relatively high yield suitable for use on an industrial scale.
The present invention is also directed to synthetic intermediates, for example 2-
(2,2,2-trifluoroacetyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1, 2,3,4-
tetrahydroisoquinoline-7-sulfonamide, a compound of Formula (III), wherein Pg =
COCF3, that are useful in the preparation of the 2-(cyclohexylmethyl)-/V-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide and salts
thereof.
DETAILED DESCRIPTION OF THE INVENTION
Definitions and Abbreviations
As used above, and throughout the description of the invention, the following
abbreviations, unless otherwise indicated, shall be understood to have the following
meanings:
HPLC high performance liquid chromatography
kg kilogram
L liter
mL milliliter
MTBE methyl f-butyl ether
The term "pharmaceutically acceptable" refers to those compounds, materials,
compositions, carrier agents, bulking agents, solvents, diluents and other
excipients which are, within the scope of sound medicinal judgment, suitable for
contact with humans or other mammals without undue toxicity, irritation, allergic
response and the like, commensurate with a reasonable benefit/risk ratio.
A process of the invention for preparing 2-(cyclohexylmethyl)-/V-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide, or a
pharmaceutically acceptable salt thereof, or a solvate or hydrate of a
pharmaceutically acceptable salt comprises:
a) coupling (-)-2-(2-aminoethyl)-1-methylpyrrolidine with an amine-protected
tetrahydroquinoline-7-sulfonyl chloride to give an amine-protected A/-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide;
b) deprotecting the amine-protected /V-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide to give A/-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide or a salt
thereof;
c) reductively aminating /V-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1, 2,3,4-
tetrahydroisoquinoline-7-sulfonamide or a salt thereof with
cyclohexanecarboxaldehyde to form 2-(cyclohexylmethyl)-/V-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide;
d) optionally reacting 2-(cyclohexylmethyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide, with a stoichiometric amount or
an excess of a salt-forming acid in a solvent to form a salt or a hydrate or solvate
thereof; and
e) optionally recrystallizing the product of step d).
In one aspect of the invention, processes for preparing the 2-(cyclohexylmethyl)-/V-{2-
[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide or a
pharmaceutically acceptable salt, solvate, or hydrate thereof, or a solvate or hydrate
of a pharmaceutically acceptable salt and the intermediates that are useful for
preparing such compounds are outlined in Scheme 1:
Scheme 1:
(la)
(salt)
The processes for preparing 2-(cyclohexylmethyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide, or a pharmaceutically
acceptable salt thereof, or a solvate or hydrate of a pharmaceutically acceptable salt,
as outlined in Scheme 1, comprise:
a) coupling (-)-2-(2-aminoethyl)-1-methylpyrrolidine with amine-protected
tetrahydroquinoline-7-sulfonyl chloride to give a compound of Formula (III), amineprotected
A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-
sulfonamide, wherein Pg represents an amine protecting group;
b) deprotecting the compound of Formula (III) to give the compound of
Formula (II), A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-
sulfonamide or a salt thereof;
c) reductively aminating the compound of Formula (II) with
cyclohexanecarboxaldehyde to give a compound of Formula (I), 2-(cyclohexylmethyl)-
A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide;
d) optionally reacting the compound of Formula (I) with a stoichiometric
amount or an excess of a salt-forming acid in a solvent to form a salt, or a hydrate or
solvate thereof, of Formula (la), 2-(cyclohexylmethyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide addition salt; and
e) optionally recrystallizing the product of step d).
A particular process of the invention for preparing 2-(cyclohexylmethyl)-A/-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide, or a
pharmaceutically acceptable salt thereof, or a solvate or hydrate of a
pharmaceutically acceptable salt, comprises:
a) coupling (-)-2-(2-aminoethyl)-1-methylpyrrolidine with an amine-protected
tetrahydroquinoline-7-sulfonyl chloride in the presence of a base to give an amineprotected
A/-{2-[(2S)-1 -methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-
sulfonamide;
b) deprotecting the amine-protected A/-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide under basic conditions and in a
solvent selected from an alcohol and a combination of water with an ethereal solvent,
to give /V-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-
sulfonamide or a salt thereof;
c) reductively aminating /V-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide or a salt thereof with
cyclohexanecarboxaldehyde in the presence of a reducing agent and in a solvent
selected from an alcohol and a combination of water with an ethereal solvent, to form
2-(cyclohexylmethyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide;
d) optionally reacting 2-(cyclohexylmethyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide, or a hydrate or solvate thereof,
with a stoichiometric amount or an excess of a salt-forming acid in a solvent to form a
salt or a hydrate or solvate thereof; and
e) optionally recrystallizing the product of step d).
Another particular process of the invention for preparing 2-(cyclohexylmethyl)-/V-{2-
[(2S)-1 -methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide, or a
pharmaceutically acceptable salt thereof, or a solvate or hydrate of a
pharmaceutically acceptable salt, comprises:
a) coupling (-)-2-(2-aminoethyl)-1-methylpyrrolidine with 2-(2,2,2-
trifluoroacetyl)-1,2,3,4-tetrahydroquinoline-7-sulfonyl chloride to give 2-(2,2,2-
trifluoroacetyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-
7-sulfonamide;
b) deprotecting 2-(2,2,2-thfluoroacetyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide to give A/-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide, or a salt
thereof;
c) reductively aminating A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide, or a salt thereof with
cyclohexanecarboxaldehyde to form 2-(cyclohexylmethyl)-/V-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide;
d) optionally reacting 2-(cyclohexylmethyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide, with a stoichiometric amount or
an excess of a salt-forming acid in a solvent to form a salt, or a hydrate or solvate
thereof; and
e) optionally recrystallizing the product of step d).
For Scheme 1:
Amine-protected N-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide is prepared in step a) from a coupling between
(-)-2-(2-aminoethyl)-1-methylpyrrolidine and an amine-protected 1,2,3,4-
tetrahydoisoquinoline-7-sulfonyl chloride, such as 2-(2,2,2-trifluoroacetyl)-1, 2,3,4-
tetrahydroisoquinoline-7-sulfonyl chloride or 1,2,3,4-tetrahydroisoquinoline-7-sulfonyl
chloride protected with other suitable amine-protecting groups (i.e., amine-protecting
groups that are stable in acid and removable under conditions that would not cleave
the sulfonamide bond). In one aspect, 2-(2,2,2-trifluoroacetyl)-A/-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide is prepared
from a coupling between (-)-2-(2-aminoethyl)-1-methylpyrrolidine and 2-(2,2,2-
trifluoroacetyl)-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonyl chloride in the presence of an
inorganic base, such as sodium hydroxide, potassium hydroxide, lithium hydroxide,
and the like, or an organic base, such as triethylamine and the like; in an inert
solvent, for example a halogenated solvent such as dichloromethane, chloroform,
,2-dichloroethane, and the like, or an ethereal solvent such as f-butyl methyl ether or
2-methyltetrahydrofuran and the like; at temperatures preferably between about 0°C
and ambient temperature, for example about 25°C.
Accordingly, one embodiment of the invention is a process for preparing 2-(2,2,2-
trifluoroacetyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-
7-sulfonamide comprising coupling 2-(2,2,2,-trifluoroacetyl)-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonyl chloride and (-)-2-(2-aminoethyl)-1-
methylpyrrolidine in the presence of a base and in an inert solvent. Another
embodiment of the invention is a process for preparing 2-(cyclohexylmethyl)-A/-{2-
[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide
comprising the step of coupling 2-(2,2,2-trifluoroacetyl)-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonyl chloride and (-)-2-(2-aminoethyl)-1-
methylpyrrolidine in the presence of a base and in an inert solvent.
Amine-protected 1,2,3,4-tetrahydoisoquinoline-7-sulfonyl chloride, such as 2-(2,2,2-
trifluoroacetyl)-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonyl chloride, may be
commercially available or otherwise may be prepared according to processes known
to those skilled in the art (see, for example, Blank, B.; Krog, A.J.; Weiner, G.;
Pendleton, R.G. J. Med. Chem. 1980, 23, 837-840). For example, amine-protected
1,2,3,4-tetrahydoisoquinoline-7-sulfonyl chlorides can be prepared by reacting the
amine-protected 1,2,3,4-tetrahydroisoquinoline with an excess of chlorosulfonic acid
in a halogenated solvent such as dichloromethane, chloroform or 1.2-dichloroethane.
In particular, 2-(2,2,2-trifluoroacetyl)-1,2,3,4-tetrahydroisoquinoline-7-sulfonyl chloride
can prepared by reacting 2-(2,2,2-trifluoroacetyl)-1 ,2,3,4-tetrahydroisoquinoline with
excess chlorosulfonic acid in either chloroform or dichloromethane at about 0°C to
about 5°C for several hours and then at room temperature for several days.
Quenching of the reaction into a mixture of chloroform or dichloromethane and
crushed ice affords a solution of the desired sulfonyl chloride, which following removal
of the solvent, can be purified by crystallization from, for example, -buty methyl
ether.
The deprotection of amine-protected A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1, 2,3,4-
tetrahydroisoquinoline-7-sulfonamide, such as 2-(2,2,2-trifluoroacetyl)-A/-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide, in step b)
may be carried out using deprotection techniques known in the art. Preferably, the
protecting group may be removed under basic conditions, for example in the
presence of an inorganic base such as potassium hydroxide, sodium hydroxide,
lithium hydroxide, and potassium carbonate. The reaction is preferably carried out in
an alcohol, such as isopropanol, methanol, and the like, or combinations of water with
an ethereal solvent, such as tetrahydrofuran and dioxane, and at a temperature
between about 0°C and about the reflux temperature of the mixture, and, more
preferably, below about 100°C.
Therefore, one embodiment of the invention is the process for preparing A/-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide comprising
deprotecting 2-(2,2,2-trifluoroacetyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1, 2,3,4-
tetrahydroisoquinoline-7-sulfonamide in the presence of a base. Another
embodiment of the invention is the process for preparing 2-(cyclohexylmethyl)-/V-{2-
[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide
comprising the step of deprotecting 2-(2,2,2-trifluoroacetyl)-A/-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide in the
presence of a base.
Step c) involves the formation of 2-(cyclohexylmethyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide by reacting /V-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide and
cyclohexanecarboxaldehyde, in the presence of a reducing agent, such as formic
acid (produced by the addition of sodium or potassium formate, and the like, for
example) in an organic solvent, for example an alcohol, such as ethanol, methanol,
isopropanol, and the like, or a combination of water with an ethereal solvent, such as
tetrahydrofuran, dioxane, and the like, or a combination of acetonitrile with water or
an alcohol with water. This reaction is preferably performed at temperatures between
about 0°C and about the reflux temperature of the mixture, and, more preferably,
below about 100°C.
Pharmaceutically acceptable salts of 2-(cyclohexylmethyl)-A/-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide, and
hydrates and solvates thereof, include conventional, non-toxic salts of 2-
(cyclohexylmethyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide, which can be formed with either inorganic
acids such as hydrochloric acid or organic acids such as benzoic acid, fumaric acid,
oxalic acid and L-tartaric acid. A pharmaceutically acceptable salt can be obtained
using standard procedures well known in the art, such as by reacting the compound
of Formula (I) with stoichiometric amounts or with an excess of the desired saltforming
acid in a suitable solvent or various combinations of solvents. For example,
an oxalate salt can be made by dissolving the compound of Formula (I) in ethanol
and adding about 1.1 equivalents of oxalic acid, and allowing the salt to form. In one
aspect of the invention, a fumarate salt is obtained. In a preferred aspect, the
fumarate salt is a difumarate monohydrate salt.
The pharmaceutically acceptable salt of 2-(cyclohexylmethyl)-/V-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide, or hydrate
or solvate thereof, is optionally recrystallized. Suitable recrystallization solvents
include, for example, isopropanol and ethanol in the presence of an antisolvent such
as toluene or acetone.
Another aspect of the invention are the processes described above further
comprising the step of formulating 2-(cyclohexylmethyl)-A/-{2-[(2S)-1-methylpyrrolidin-
2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide or a pharmaceutically
acceptable salt thereof, or a solvate or hydrate of a pharmaceutically acceptable salt,
with one or more pharmaceutically acceptable carrier agents, bulking agents,
solvents, diluents and other excipients. In one aspect, the process comprises
the step of formulating 2-(cyclohexylmethyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide difumarate monohydrate with
one or more pharmaceutically acceptable carrier agents, bulking agents, solvents,
diluents and other excipients.
The compound of Formula (V), (-)-2-(2-aminoethyl)-1-methylpyrrolidine, may be
prepared as outlined in Scheme 2.
Scheme 2:
For Scheme 2:
Step 1) entails combining 2-(2-aminoethyl)-1-methylpyrrolidine with a chiral resolving
agent, such as di-p-toluoyl-D-tartaric acid, in an alcohol, such as ethanol, methanol,
isopropanol, and the like, and combinations thereof including combinations with
water. Preferably, the solvent is a combination of ethanol and water. The reaction is
preferably performed at temperatures between about 0°C and about the reflux
temperature of the mixture, and more preferably, below about 100°C.
Racemic 2-(2-aminoethyl)-1-methylpyrrolidine starting material is commercially
available (for example, from Anichem LLC, American Custom Chemicals Inc., Acros,
Aldrich) or otherwise may be prepared according to procedures well known to those
skilled in the art. (See Turner, S.C.; Esbenshade, T.A.; Bennani, Y.L.; Hancock,
A.A. Bioorg. Med. Chem. Lett. 2003, 3, 2131-2135).
Step 2) involves removing the resolving agent by dissolving the product of step 1), for
example, (-)-2-(2-aminoethyl)-1-methylpyrrolidine, 0,0'di-p-toluoyl-D-tartaric acid salt,
in a two-phase mixture of a strong acid, such as concentrated HCI, HBr, H2S0 4, or
H3PO4, and a non-polar solvent, such as f-butyl methyl ether, isopropyl acetate, and
the like, at temperatures between about room temperature and about 100°C. In one
aspect, (-)-2-(2-aminoethyl)-1-methylpyrrolidine is isolated in aqueous solution as a
salt. In another aspect, the acidic solution of (-)-2-(2-aminoethyl)-1-methylpyrrolidine
salt is basified by the addition of a base, such as sodium hydroxide, allowing the
isolation of (-)-2-(2-aminoethyl)-1-methylpyrrolidine as the distillable free base.
The following examples present typical syntheses as described in Schemes 1 and 2.
These examples are understood to be illustrative only and are not intended to limit
the scope of the present invention in any way.
Example 1
Preparation of compound of Formula (VI): (-)-2-(2-Aminoethyl)-1-methylpyrrolidine,
0,0 -Di-p-toluoyl-D-tartaric acid salt
A stock solution of aqueous ethanol was prepared by mixing ethanol (10370 mL) and
water (2080 mL). A mixture of ,'-di-p-tolouyl-D-tartaric acid (1624 g, 4.203 mol)
and a portion of the above-described stock solution of aqueous ethanol (9050 mL)
was stirred at around 65°C under a nitrogen atmosphere. Separately, racemic 2-(2-
aminoethyl)-1-methylpyrrolidine (700 g, 5.35 mol) was dissolved in a portion of the
aqueous ethanol stock solution (3400 mL). The amine solution was then added dropwise
to the tartaric acid solution so that the temperature was maintained at about
65°C and no solids formed during the addition. The reaction was held at about 65°C
for no less than 30 min before being cooled to about 0°C. The precipitate was
collected by filtration. A stream of nitrogen was pulled through the collected solid until
no longer wet. The solid was recrystallized from ethanol (15950 mL)/water (2457 mL)
affording the desired product as a colorless solid: 1322.4 g (44%), >99.5% ee.
Example 2
Preparation of compound of Formula (V): (-)-2-(2-Aminoethyl)-1-methylpyrrolidine
A solution of HCI (296 mL, 3.55 mol) and water (517 mL) was added to a mixture of (-
)-2-(2-aminoethyl)-1-methylpyrrolidine, 0,0 -di-p-toluoyl-D-tartaric acid salt (900 g,
1.75 mol) and MTBE (3.2 L). After stirring for 45 minutes, the layers were separated.
Additional MTBE (1.6 L) was added to the aqueous layer. After stirring for about 10
minutes, the layers were separated. With stirring, 50% aqueous NaOH (476 mL, 9.19
mol) was added to the aqueous acid layer over about 35 minutes. The mixture was
stirred for about 35 minutes, then cooled to 10°C. The organic layer was separated
and distilled at reduced pressure to provided 206 g (92%) of the desired product.
Example 3
Preparation of compound of Formula (III): 2-(2,2,2-Trifluoroacetyl)-/V-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide
A solution of (-)-2-(2-aminoethyl)-1-methylpyrrolidine (172 g, 1.345 mol) in water (400
mL) was prepared and added to a solution of 2-(2,2,2-trifluoroacetyl)-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonyl chloride (400 g, 1.22 mol) in 2-
methyltetrahydrofuran (1600 mL) at a controlled rate to maintain the reaction
temperature between -5°C and 5°C. Upon completion of the coupling, the reaction
mixture was washed once with an aqueous potassium carbonate solution prepared
from potassium carbonate (220 g, 1.59 mol) and water ( 1 L) to liberate the free base
of the product. Most of the 2-methyltetrahydrofuran was removed by vacuum
distillation to the minimum stirred volume and replaced with ethanol (2 L). The
distillation was resumed and continued to the minimum stirred volume. The ethanolic
solution of 2-(2,2,2-trifluoroacetyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide was diluted with ethanol ( 1 132 mL) to obtain a
30 wt % solution of product, assuming a 97% yield, and carried forward into the next
step.
Example 4
Preparation of a compound of Formula (II): A/-{2-[(2S)-1-Methylpyrrolidin-2-yl]ethyl}-
1,2,3,4-tetrahydroisoquinoline-7-sulfonamide Dihydrochloride Sesquihydrate
A 45.7% solution of potassium hydroxide (105 g , 0.855 mol) was added to a 30.66%
(w/w) ethanolic solution of 2-(2,2,2-trifluoroacetyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide (1023.6, 0.748 mol) at a rate
that limited the temperature to no more than 45°C. After cooling to room
temperature, the reaction was monitored by HPLC. Upon completion of the
deprotection, the reaction was acidified by the dropwise addition of concentrated
hydrochloric acid (194 mL, 2.35 mol). The precipitated KCI was removed by filtration
at between 39-51 °C. The reactor was rinsed with ethanol (300 mL) which in turn was
used to rinse the collected KCI. The ethanol filtrates were combined and returned to
the reactor. The reactor was heated to approximately 55°C and 2-
methyltetrahydrofuran (375 mL) was added. Seed crystals of A/-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide
dihydrochloride sesquihydrate ( 1 g) were added. After cooling to approximately -5°C,
the product was collected by filtration. The product was rinsed with a solution
prepared from 2-methyltetrahydrofuran (200 mL), ethanol (200 mL) and water (5 mL).
Following drying in a vacuum oven at 40°C, 277 g (87%) of W-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide
dihydrochloride sesquihydrate was isolated as a colorless solid.
Seed crystals of W-{2-[(2S)-1-Methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide dihydrochloride sesquihydrate can be obtained
following general procedures known to those skilled in the art. Alternatively, N-{2-
[(2S)-1-Methylpyrrolidin-2-yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide
dihydrochloride sesquihydrate can readily be prepared as described above without
the use of seed crystals.
Example 5
Preparation of a compound of Formula (I): 2-(Cyclohexylmethyl)-/V-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide
A/-{2-[(2S)-1-Methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide
dihydrochloride sesquihydrate (92 g, 0.217 mol) was reacted with potassium formate
(33.6 g, 0.399 mol) and cyclohexanecarboxaldehyde (39 mL, 0.322 mol) in hot SDA
3C ethanol (460 mL). The reaction vessel was heated to about 72°C, with release of
C0 2. The reaction mixture was monitored for completion by HPLC. Upon
completion, the reaction was cooled to about 25°C, and ethanol was removed by
vacuum distillation and azeotropic removal with added water (280 mL). The
hydrochloride salt of the product was formed by the addition of concentrated
hydrochloric acid (37.3 mL, 0.451 mol), and the aqueous solution was washed with
isopropyl acetate (280 mL). The aqueous layer was basified with a solution of
potassium carbonate (92 g, 0.666 mol) in water (100 mL). The aqueous alkaline
solution was extracted with isopropyl acetate (460 mL). The isopropyl acetate layer
was washed with water (3 x 460 mL), and the isopropyl acetate was removed by
vacuum distillation and replaced with SDA 3C ethanol (460 mL). The resulting clear
yellow-tinged solution contained 85.7 g (91%) of 2-(cyclohexylmethyl)-/V-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide in 120.4 g of
solution.
Example 6
Preparation of a Compound of Formula (la): 2-(Cyclohexylmethyl)-/V-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide difumarate
monohydrate
A solution of 2-(cyclohexylmethyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1, 2,3,4-
tetrahydroisoquinoline-7-sulfonamide (532 g, 1.27 mol) in SDA 3C ethanol (1056 mL)
was added to a suspension of fumaric acid (302 g, 2.60 mol) in water (624 mL). The
resulting solution was diluted with acetone (4 L) then cooled and seeded with milled
2-(cyclohexylmethyl)-W-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide difumarate monohydrate (4.2 g). After seeding,
the mixture was stirred to allow crystal growth then further diluted with acetone (1990
mL). After cooling, the product was collected by filtration and washed with acetone
( 1.500 L). Filtration was conducted by portionwise loading of acetone into the filterdryer.
After loading of each portion of acetone (1.5L), stirring was turned on at 2.6
rpm to ensure good contact between product and acetone. The product was dried in
a vacuum oven at 40°C with nitrogen purge and vacuum (residual pressure 400
mBar), then allowed to re-hydrate at room temperature in the air to yield 684.7 g (85.8
%) of 2-(cyclohexylmethyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide difumarate monohydrate.
Seed crystals of 2-(cyclohexylmethyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide difumarate monohydrate can be obtained
following general procedures known to those skilled in the art in view of the abovedescribed
procedure. Alternatively, 2-(cyclohexylmethyl)-/V-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide difumarate
monohydrate can readily be prepared as described above without the use of seed
crystals.
CLAIMS
What is claimed is:
1. A process for preparing 2-(cyclohexylmethyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide, or a pharmaceutically
acceptable salt thereof, or a solvate or hydrate of a pharmaceutically acceptable salt
comprising:
a) coupling (-)-2-(2-aminoethyl)-1-methylpyrrolidine with an amine-protected
tetrahydroquinoline-7-sulfonyl chloride to give an amine-protected A/-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide;
b) deprotecting the amine-protected A/-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide to give /V-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide or a salt
thereof;
c) reductively aminating A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide or a salt thereof with
cyclohexanecarboxaldehyde to form 2-(cyclohexylmethyl)-A/-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide;
d) optionally reacting 2-(cyclohexylmethyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide, or a hydrate or solvate thereof,
with a stoichiometric amount or an excess of a salt-forming acid in a solvent to form a
salt or a hydrate or solvate thereof; and
e) optionally recrystallizing the product of step d).
2. The process according to claim 1, wherein the amine-protected
tetrahydroquinoline-7-sulfonyl chloride is 2-(2,2,2-trifluoroacetyl)-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonyl chloride and the amine-protected /V-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide is 2-(2,2,2-
trifluoroacetyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-
7-sulfonamide.
3. The process according to claim 1, wherein the coupling in step a) is performed
in the presence of an inorganic or organic base and in an inert solvent.
4. The process according to claim 3 , wherein the base is selected from the group
consisting of sodium hydroxide, potassium hydroxide, lithium hydroxide and
triethylamine.
5 . The process according to claim , wherein deprotecting in step b) is performed
under basic conditions and in an alcohol.
6 . The process according to claim 1, wherein the reductive amination of step c) is
performed in an organic solvent in the presence of a reducing agent.
7. The process according to claim 6 , wherein the reducing agent is formic acid.
8. The process according to claim 1, wherein the salt formed in step d) is a
pharmaceutically acceptable salt.
9. The process according to claim 1, further comprising formulating 2-
(cyclohexylmethyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1, 2,3,4-
tetrahydroisoquinoline-7-sulfonamide or a pharmaceutically acceptable salt thereof,
or a solvate or hydrate of a pharmaceutically acceptable salt, with one or more
pharmaceutically acceptable carrier agents, bulking agents, solvents, diluents
and other excipients.
10. The process according to claim 1, wherein the salt-forming acid in step d)
is fumaric acid to provide 2-(cyclohexylmethyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide difumarate monohydrate.
1. The process according to claim 10, further comprising formulating 2-
(cyclohexylmethyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1, 2,3,4-
tetrahydroisoquinoline-7-sulfonamide difumarate monohydrate with one or more
pharmaceutically acceptable carrier agents, bulking agents, solvents, diluents
and other excipients.
12. The process according to claim 1, comprising:
a) coupling (-)-2-(2-aminoethyl)-1-methylpyrrolidine with 2-(2,2,2-
trifluoroacetyl)-1,2,3,4-tetrahydroquinoline-7-sulfonyl chloride to give 2-(2,2,2-
trifluoroacetyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-
7-sulfonamide;
b) deprotecting 2-(2,2,2-trifluoroacetyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide to give A/-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide, or a salt
thereof;
c) reductively aminating A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1, 2,3,4-
tetrahydroisoquinoline-7-sulfonamide, or a salt thereof with
cyclohexanecarboxaldehyde to form 2-(cyclohexylmethyl)-A/-{2-[(2S)-1-
methylpyrrolidin-2-yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide;
d) optionally reacting 2-(cyclohexylmethyl )-N -{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide with a stoichiometric amount or
an excess of a salt-forming acid in a solvent to form a salt, or a hydrate or solvate
thereof; and
e) optionally recrystallizing the product of step d).
13. The process according to claim 12, further comprising formulating 2-
(cyclohexylmethyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide or a pharmaceutically acceptable salt, or a
solvate or hydrate of a pharmaceutically acceptable salt, with one or more
pharmaceutically acceptable carrier agents, bulking agents, solvents, diluents
and other excipients.
14. The process according to claim 12, wherein the salt-forming acid in step
d) is fumaric acid to provide 2-(cyclohexylmethyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide difumarate monohydrate.
15. The process according to claim 14, further comprising formulating 2-
(cyclohexylmethyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide difumarate monohydrate with one or more
pharmaceutically acceptable carrier agents, bulking agents, solvents, diluents
and other excipients.
16. The process according to claim 1, comprising:
a) coupling (-)-2-(2-aminoethyl)-1-methylpyrrolidine with an amine-protected
tetrahydroquinoline-7-sulfonyl chloride in the presence of a base to give an amineprotected
A/-{2-[(2S)-1 -methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-
sulfonamide;
b) deprotecting the amine-protected A/-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfona under basic conditions and in a
solvent selected from an alcohol and a combination of water with an ethereal solvent,
to give A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-
sulfonamide or a salt thereof;
c) reductively aminating A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide or a salt thereof with
cyclohexanecarboxaldehyde in the presence of a reducing agent and in a solvent
selected from an alcohol and a combination of water with an ethereal solvent, to form
2-(cyclohexylmethyl)-/V-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1, 2,3,4-
tetrahydroisoquinoline-7-sulfonamide;
d) optionally reacting 2-(cyclohexylmethyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide with a stoichiometric amount or
an excess of a salt-forming acid in a solvent to form a salt or a hydrate or solvate
thereof; and
e) optionally recrystallizing the product of step d).
17. The compound 2-(2,2,2-trifluoroacetyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide.
18. A process for preparing 2-(2,2,2-trifluoroacetyl)-A/-{2-[(2S)-1-methylpyrrolidin-
2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide comprising coupling 2-(2,2,2-
trifluoroacetyl)-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonyl chloride and (-)-2-(2-
aminoethyl)-1-methylpyrrolidine in the presence of a base and in an inert solvent.
19. A process for preparing 2-(cyclohexylmethyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1,2,3,4-tetrahydroisoquinoline-7-sulfonamide, comprising the step of
coupling 2-(2,2,2-trifluoroacetyl)-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonyl chloride and
(-)-2-(2-aminoethyl)-1-methylpyrrolidine in the presence of a base and in an inert
solvent.
20. A process for preparing W-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide comprising deprotecting 2-(2,2,2-
trifluoroacetyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-
7-sulfonamide in the presence of a base.
2 1. A process for preparing 2-(cyclohexylmethyl)-A/-{2-[(2S)-1-methylpyrrolidin-2-
yl]ethyl}-1 ,2,3,4-tetrahydroisoquinoline-7-sulfonamide, comprising the step of
deprotecting 2-(2,2,2-trifluoroacetyl)-A/-{2-[(2S)-1 -methylpyrrolidin-2-yl]ethyl}-1 ,2,3,4-
tetrahydroisoquinoline-7-sulfonamide in the presence of a base.