FORM 2
THE PATENTS ACT, 1970
(39 of 1970)
&
THE PATENTS RULES, 2003
Complete Specification
[See Sections 10 and rule 13]
Title: Process for the Preparation of Cabazitaxel And Its Intermediates
Applicant: (a) INTAS Pharmaceuticals Limited
(b) Company Registered under Indian Company ACT
(c) 2nd Floor, Chinubhai Centre, Ashram Road, Ahmedabad 380009 Gujarat. India
The following specification particularly describes the invention and the manner in which this is to be performed:

FIELD OF THE INVENTION
The present invention relates to a novel process for the preparation of Cabazitaxel and its intermediates.
BACKGROUND OF THE INVENTION
Cabazitaxel exhibits notable anticancer and antileukaemic properties. Cabazitaxel. chemically known as 4-acetoxy-2a-benzoyioxy-5β,20-epoxy-l-hydroxy-7β 10β-dimer.hoxy-9-oxo-tax-l l-en-13a-yl (2R,3S)-3-tert-butoxycarbonylarnino-2-hydroxy-3-phenylpropionate and is represented by the following structural formula:

The compound was disclosed in US 5,847.170 (hereinafter referred as US''170). It is sold under brand name Jevtana as its acetone solvate. Cabazitaxel is prepared according to the method which is described more particularly in US' 170.
Although Cabazitaxel is a very important second line treatment for the metastatic CRPC, there are still limited reports on the synthesis of Cabazitaxel. Aventis reported the first synthetic route of Cabazitaxel in US' 170 starting from 10-deacetylbaccatin III (10-DAB). The synthesis consisted of more than five steps with a very low reported yield.

US 5,962,705 disclosed a process for the taxoid derivatives using alkylating agents such as alkyl halide, alkyl sulfate, oxonium in the presence of an anionization agent.
CM 102060815 provided a method for the conversion of Docetaxel to Cabazitaxel by using dimethylsulfate as an alkylating agent in a weakly alkaline organic solvent (pyridine).
CN 102285947 reported the synthesis of Cabazitaxel by imethylating the 7 and 10-O.H in 10-DAB simultaneously to furnish 7.10-dimethyl-10-DAB. which was then coupled with a protected (3R,4S)-(3-lactam followed by deprotection of the 2;-OH. the total yield is approximately 18.0% for 3 steps.
Thus, there is a need for developing a process for preparation of cabazitaxel and its key intermediates which is not only feasible at industrial scale but also meets economics of scale in terms of yield.
OBJECTS OF THE INVENTION
It is an object of the present invention is to provide a novel process for the preparation of Cabazitaxel and its key intermediate.
Another object of the present invention is to provide a process for preparing
Cabazitaxel using chiral auxiliaries.
Another object of the present invention is to provide a process for preparing Cabazitaxel from Docetaxel.

SUMMAJRY OF THE INVENTION

in which X represents H or side chain of formula (III)

In one aspect the present invention provides a process for preparation of cabazitaxel (T) comprising methylation of compound of formula (II)
Z represents a hydroxy protecting group, R 1 is C(O)OC(CH3)3,
Using a methylating agent, methyl trifluoromethansulfonate to get compound of

converting,compound of formula (IV) to cabazitaxel (I).
formula (IV)

Yet another aspect of the present invention provides process to prepare cabazitaxel
(I) comprising
reacting a compound of formula (II) wherein Z= triethyl silyl
with methyl trifluoromethansulfonate to obtain compound of formula (XII)

converting compound of formula (XII) to cabazitaxel
In another aspect the present invention provides a process to prepare cabazitaxel (I) comprising selective 2' deprotection of compound of formula (XIII.)

In accordance with another aspect of the present invention there is provided a process for preparation of cabazitaxel where novel and chiral bis lactam of formula (V)


Rl is defined above. Ar is a phenyl group and L is a cleavable linker is reacted with a suitable taxane precursor of formula (VI)



to give a compound of formula (VII)
cleaving the linker from compound of formula (VII) to get cabazitaxe! (I).
In a further aspect the present invention provides a process to prepare cabazitaxel which comprises reacting compound of formula (VIII)


with methyl trifluoromethansulfonate to obtain compound of formula (VI)

reacting compound of formula (VI) with compound of formula (IX)

BOC=CO(0)C(CH3)3, W=alkyl of 1-30 carbon atom to get compound of formula (X)


converting compound of formula (X) to cabazitaxel (I) DETAILED DESCRIPTION
The present invention provides process for preparing cabazitaxel.
Accordingly, the present invention provides a process for methylation of the two hydroxyl groups at 7 and 10 position of 10-deacetylbaccatin or derivatives thereof of formula (II)

In which. X represents H or side chain of formula (III)
wherein Z represents hydroxy protecting group and Rl is C(0)OC(CH3)3. using methyl trifuoromethanesulfonate as methylating agent. This process includes direct conversion of docetaxel to cabazitaxel or methylation of 10-deacetyl baccatin and further conversion of 7.10 dimethyl baccatin to cabazitaxel.
More specifically, according to one of the aspects of the present invention, processes for preparation of cabazitaxel using docetaxel are described.

is reacted with methyl trifluoromethansulfonate to get compound of formula (XH)

Accordingly, in an embodiment the present invention provides a process to prepare cabazitaxel wherein compound of structural formula (XI)

The compound of formula (XII) can be converted to cabazitaxel The process can be further exemplified as following scheme

In another aspect the present invention provides a process for preparing cabazitaxel by selective deprotection of compound of formula (XI !I)

The process can be further exemplified as following scheme


In the process of present invention protecting group can be selected from any suitable hydroxy protecting group preferably a silyl protecting group such as triethyl silyl is used for the purpose of present invention.
The protection reaction or can be carried out in presence of a suitable solvent and base. Solvent can be selected from any suitable solvent such from the group comprising of nitrile, chlorinated hydrocarbon, polar aprotic solvent, ethers and mixture thereof. Base can be selected from inorganic such as alkali metal or alkali earth-metal carbonate or bicarbonates, metal hydroxide, organic base can be selected from group consisting of alkyl amine like triethyl amine, morpholine. pyridine like dimethyl amino pyridine, piperidine or like.
Protection of 2' -OH is followed by methylation of 7 and 10 hydroxyl group. The methylation is carried out by using methyl trifluoromethansulfonate (methyl triflate). The reaction can be carried out in presence of solvent and base. The solvent used in methylation reaction can be selected from any suitable organic solvent such as

solvent selected from the class of ester, ketone, ether, cyclic ether or like. The base can be selected from any base suitably used in methylation preferably salts hexamethyldisilazide are used in present process. 2'7.10 methylation can also be carried out in presence of suitable solvent and base.
The protected cabazitaxel thus prepared can be subjected to deprotection or selective deprotection in presence of base. Preferably a mild base such as tetrabutyl ammonium fluoride is used.
The above process of protection of -OH group, methylation and deprotection or 2',7,10 methylation and 2'selective deprotection to get cabazitaxel can be carried out in a single step i.e. without isolating the intermediate stages or in multiple steps.
Another aspect of the present invention is to provide a process for the preparation of Cabazitaxel, where novel, chiral bis-lactams of formula (V)

Rl is defined above, Ar is a phenyl group and Lisa cleavable linker is reacted with a suitable taxane precursor having a free C-13 hydroxy group.
Accordingly, cabazitaxel can be prepared by reacting taxane precursor of formula (VI):



with a compound of formula (V) to give a compound of formula (VII)
Cabazitaxe! is released from the compound of formula (VII) by cleaving the linker.
The cleavable linker L can be chiral or non-chiral, preferably selected from the group consisting of hydrolysable ketals. acetals, silyt, esters, diesters and hydrogenolysable benzyl group.
Further L can be selected form compound of structural formula

wherein Rc and R'c, identical or different are alkyl, aryl or hydrogen. Rd and R'd, identical or different are alkyl, aryl or hydrogen, Rf and R'f, identical or different are alkyl. aryl or hydrogen, Rg and R'g. identical or different are alkyl, aryl or hydrogen; W is an alkyl. Further W can be an alkyl of I -30 carbon atoms.
In an embodiment the taxane precursor can be prepared by reacting 10-deacetyl baccatin III of formula (VIII)

with methylating agent, preferably methyl trifiuoromethansulfonate. The methylation reaction is carried out in presence of a base preferably the base used herein is salt of hexamethyl disilazide like sodium, potassium, lithium hexamethyl disilazide. The 7,10-dimethoxy-lO-deacetyl baccatin- III (VI) thus prepared

is reacted with N-boc-bis lactam of formula (fX)


BOC=CO(0)C-(CH3), W=alkyl of 1-30 carbon atoms to give compound of formula (X)

The reaction is carried out in presence of a suitable solvent and base. The process comprises reacting in presence of solvent selected from class of ether, cyclic ether, ester, halogenated solvent, hydrocarbon, protic or aprotic solvent. The base can be selected from any suitable base for such reaction preferably the present invention uses salt of hexa methyl disilazide such as sodium potassium or lithium hexamethyl disilazide.

Compound of formula (X) is subjected to a reaction for cleavage of linker to get cabazitaxel. The reaction can be carried out in presence of solvent and base. Solvent can be selected from any suitable solvent like ether such as tetrahydrofuran, ketone such as acetone, ester such as ethyl acetate, alkane such as heptane, alcohol such as, isopropyl alcohol, nitrile such acetonitrile or like. The solvent can be used as single solvent, as mixture or as a solvent antisolvent combination thereof.
Cabazitaxel thus obtained can be further purified by treating with solvents, such as acetonitrile. diethyl ether, benzytoxy methyl ether, benzyl ether, petroleum ether. ester such as ethyl acetate, alcohol such as ethanol. methanol, isopropanol either as a single solvent or a mixture of solvents in different ratios, preferably in acetonitrile and an alcohol preferably methanol. Cabazitaxel could also be purified by column chromatography but yields may be at lower side.
Following are the specific examples describing the invention. These examples are not intended to limit the scope of the invention in any way.
EXAMPLES:
Example 1: Preparation of (2'-Tes-docetaxel) (XI)
To a mixture of docetaxel (807 mg) in 25 ml of dichloromethane at 0°C was added dimethylaminopyridine, (122 mg) and triethylamine (0.278 ml) followed by triethyl silyl chloride (150mg). The product was isolated by extraction followed by evaporation of solvent, purified over silica gel using hexane/acetone as eluent to obtain approximately 800 mg of 2'-Tes-docetaxel, in approximately 90% yield.
Example 2: Preparation of 2'-Tes-7,10 dimethoxy-docetaxel (XII)
To 2'-Tes-docetaxel (500 mg) in 10 ml of THF at -30 to -50°C was added LiHMDS, (1ml) and methyl trifluoromethansulfonate (0.120 ml). The product was isolated by

extraction followed by evaporation of solvent, purified over silica gel using dichloromethane/methanol as eluent to obtain approximately 464 mg of 2'-Tes-7.10-dimethoxy-docetaxel. in approximately 90% yield
Example 3: Preparation of cabazitaxel (I)
To 2'-Tes-7.10-dimethoxy-Tes-docetaxel (380mg) in 10 ml of tetrahydrofuran at room temperature was added tetrabutylammoniurnfluoride. (800ul). The product is isolated by extraction and evaporation of solvent, purified over silica gel using dichloromethane/methanol as eluent to obtain approximately 275 mg of Cabazitaxel, in approximately 80% yield.
Example 4: Preparation of 2',7,10-trimethoxy-docetaxel (XIII)
To Docetaxel (2 g) in 25 ml of tetrahydrofuran at -30 to -50°C was added LiHMDS, (7.4 ml) and methyl trifluoromethansulfonate (0.815 mi). Followed by extraction and evaporation of solvent, purified over silica gel using dichloromethane/methanol as eluent to obtain approx 1.7 g of 2'7,10-trimethoxy-docetaxel. in approximately 80% yield
Example 5: Preparation of Cabazitaxel (I)
To trimethoxy-Docetaxel (850 mg) in 25 ml of dichloromethane at 0°C was added aqueous solution of HBr (2 ml) and allowed the reaction to complete, product was isolated by extraction and evaporation of solvent, purified over silica gel using dichloromethane/methanol as eluent to obtain approximately 600 mg of cabazitaxel. in approximately 72% yield
Example 6: Preparation of 7,10-dimethoxy-10-deacety! baccatin III (VI)
Under Argon, 2.43 g of deacetyl baccatin in 50 ml of tetrahydrofuran was cooled to -30 to -50C followed by the addition of 1.23 ml of Methyl triflate and 9.8 ml of

1M LiHMDS. Product was isolated by extraction followed by evaporation of solvent, purified over silica gel using dichloromethane/methanol as eluent to obtain approximately 2.2 g of 7,10-dimethoxy-deacety] baccatin. in approximately 87% yield
Example 7: Preparation of compound of formula (X)
To 8.2 g. of 7,10-dimethoxy-deacetyl baccatin in a mixture of tetrahydrofuran and dimethyl form amide was added 7.363 g N-Boc-bis-lactam and 15 ml of I M LiHMDS at -20°C to -30C under argon product was isolated by extraction, followed by evaporation of the solvent to afford approximately 17 g of dimer compound of formula (XI).
Example 8: Preparation of cabazitaxel (I)
To 3 g of the dimer (XI), in 20 ml of tetrahydrofuran, at 0°C was added 3.8 ml of tetrabutyl ammonium fluoride and left stirring under argon. Product was isolated by extraction followed by evaporation of the solvent to afford approximately 3.17 g of Cabazitaxel.

We Claim:

in which X represents H or side chain of formula (III)

1. A process to prepare cabazitaxel (I.) comprising a step of: reacting a compound of formula II
Z represents a hydroxy protecting group. Rl is C(O)OC(CH3)3
with methyl trifluoromethansulfonate to get compound of formula (IV)

2. A process to prepare cabazitaxel comprising a step of: reacting compound of formula II


wherein X represents side chain of formula (III). Z= triethyl silyl

with methyl trifluoromethansulfonate to get a compound of formula (XII.)

3. A process to prepare cabazitaxel comprising a step of selective 2' deprotection of compound of formula (XIII)


4. A process to prepare cabazitaxel (I) comprising a step of reacting compound of formula (V)

to get compound of formula (VII)

wherein Rl as defined, Ar is a phenyl group and L is a cleavable linker with a suitable taxane precursor of formula


5. A process to prepare cabazitaxel comprising steps of:

to get compound of formula (VI)

a. reacting a compound of formula (VIII) with methyl trifluoromethansulfonate

to get compound of formula (X)
b, reacting compound of formula (VI) with compound of formula (JX)


wherein w= alkyl having C1-C30
c. treating compound of formula (X) in presence of solvent and base to obtain cabazitaxel (I)
6. A process according to any of the preceding claim wherein solvent selected for protection is selected form the group consisting of nitrile. chlorinated hydrocarbon, polar aprotic solvent, ester, ether, cyclic ether and mixture thereof.
7. A process according to any of preceding claim wherein methylation is carried in presence of base.
8. A process according to claim 7, wherein base is selected from lithium hexamethyl disilazide, sodium hexamethyldisilazide and potassium hexamethyl disilazide.