FORM 2
THE PATENTS ACT 1970
(Act 39 of 1970)
COMPLETE SPECIFICATION
(SECTION 10, rule 13)
"PROCESS FOR THE PREPARATION OF (E)-7-[4-(4-FLUOROPHENYL)-6-
ISOPROPYL-2-[METHYL(METHYLSULFONYL)AMINO]PYRIMIDIN-5-YLl(3R,5S)-
3,5-DIHYDROXYHEPT-6-ENOlC ACID CALCIUM SALT."
Glenmark Pharmaceuticals Limited, an Indian Company,
registered under the Indian company's Act 1957 and
having its registered office at
B/2, Mahalaxmi Chambers, 22, Bhulabhai Desai Road
Post Box No. 26511 Mumbai- 400 026, India
THE FOLLOWING SPECIFICATION DESCRIBES THE NATURE OF THE INVENTION AND THE MANNER IN WHICH IT IS TO BE PERFORMED


PRIORITY
This application claims the benefit under 35 U.S.C. §119 to Provisional Application No. 60/561,732,.filed April 13, 2004 and entitled "PROCESS FOR THE PREPARATION OF RUSUVASTATIN", and from Indian Provisional Application 1244/Mum/2003, filed on December 4, 2003, the contents of which are incorporated by reference herein. BACKGROUND OF THE INVENTION
1. Technical Field
The present invention generally relates to an improved process for the preparation of pyrimidine derivatives such as 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) inhibitors and their intermediates. More specifically, the present invention relates to an improved process for the preparation of rosuvastatin and its intermediates using Wittig reagents.
2. Description of the Related Art
The present invention is directed to an improved process for the preparation of pyrimidine derivatives such as rosuvastatin (also known as bis[(E)-7-[4-(4-fluorophenyl)-6-isopropyl-2-[methyl(methylsulfonyl)amino]pyrimidin-5-yl](3R)5S)-3,5-dihydroxyhept-6-enoic acid]). Rosuvastatin has the following structural formula I:

Generally, rosuvastatin is a synthetic lipid-lowering agent that acts as an inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase (HMG-CoA Reductase inhibitor). This enzyme catalyzes the conversion of HMG-CoA to mevalonate, an early and rate-limiting step in cholesterol biosynthesis. HMG-CoA reductase inhibitors are commonly referred to as "statins." Statins are therapeutically effective drugs used for reducing low density lipoprotein (LDL) particle concentration in the blood stream of patients at risk for cardiovascular disease. Rosuvastatin is used in the treatment of hypercholesterolemia (heterozygous familial and
2

nonfamilial) and mixed dyslipidemia (Fredrickson Type IIa and IIb). Rosuvastatin calcium is sold under the brand name CRESTOR .
U.S. Patent No. 5,260,440 to Hirai et al, discloses pyrimidine derivatives such as rosuvastatin, its calcium salt (2:1) and its lactone form. The '440 patent also discloses a process for the preparation of pyrimidine derivatives in a four step reaction scheme.
The disadvantages of the prior art include multiple steps resulting in difficult preparations, the use of expensive reagents, reagents that are difficult to use on a commercial scale, the use of low temperatures that make the process more difficult to perform, and time consuming and expensive techniques for purification.
Accordingly, there remains a need for an improved process for the preparation of rosuvastatin that eliminates and reduces the problems of the prior art on a commercial scale in a convenient and cost efficient manner.
SUMMARY OF THE INVENTION
In one embodiment of the present invention, a process for the preparation of pyrimidine derivatives is provided comprising reacting a Wittig reagent of the general formula

wherein R is an alkyl of from 1 to 10 carbon atoms, aryl or arylalkyl, R1 is a substituted or
3
unsubstituted hydrocarbon group, R and R are the same or different and are hydrogen or a substituted or unsubstituted hydrocarbon group; Z is sulfur, oxygen, sulfonyl, or imino which may be substituted by formyl, acetyl, propionyl, butyryl, isobutyryl, valeryl, isovaleryl, amino substituted by sulfonyl or alkylsulfonyl, and sulfonyl substituted by alkyl, amino or alkylamino and X is a halogen; with an aldehyde of the general formula


wherein R4 is hydrogen, a lower alkyl or a cation capable of forming a non-toxic pharmaceutically acceptable salt and each R5 are the same or different and are hydrogen or a hydrolyzable protecting group, or each R5, together with the oxygen atom to which each is bonded, form a hydrolyzable cyclic protecting group, or each R5 is bonded to the same substituent which is bonded to each oxygen atom to form a hydrolyzable protecting group; in the presence of a base to provide a pyrimidine derivative of the general formula

wherein R, R1, R2, R3, R4, R5, X and Z have the aforestated meanings or a racemic mixture, an enantiomer, a diastereoisomer or a mixture thereof.
In a second embodiment of the present invention, a process for the preparation of pyrimidine derivatives comprising reacting a Wittig reagent of the general formula

wherein R, R1, R2. R3 Z and X have the aforestated meanings; with an aldehyde of the general formula

wherein R has the aforestated meaning and R5 is a hydrolyzable protecting group; in the presence of a base to provide a pyrimidine derivative of the general formula
4

wherein R3 R1, R2, R3, R4, R5, and Z have the aforestated meanings or a racemic mixture, an enantiomer, a diastereoisomer or a mixture thereof.
In a third embodiment of the present invention, a process for the preparation of an intermediate of rosuvastatin is provided. The intermediate tert-butyl[E]-(6-{2-[4-(4-fluorophenyl]-6-isopropyl-2-[methyl(methylsulfonyl)amino]pyrimidin-5-yl]vinyl}-(4R,6S)-232-dimethyl[l,3]dioxan-4-yl)acetate (BEM) can be prepared by the process comprising reacting a Wittig reagent of the general formula:

wherein R and X have the aforestated meanings; with tert-butyl-2-[(4R,6S)-6-formyI-2,2-dimethyl-l,3-dioxan-4-yl]acetate (BFA) of the following formula:

in the presence of a base to provide BEM of the following formula:
5


In another embodiment of the present invention, a process ior the preparation or a calcium salt of rosuvastatin is provided comprising:
(a) providing BEM;
(b) contacting the BEM with an amine of the formula R5R6NH2; wherein R5 and
R6 are the same or different and can be an alkyl of from 1 to 6 carbon atoms in the presence of a
dilute acid to form the alkylammonium salt of rosuvastatin; and
(c) contacting the alkylammonium salt of rosuvastatin to with a sufficient amount
of a source of calcium to provide a calcium salt of rosuvastatin.
The advantages of the present invention include:
(1) More economical production on a commercial scale because of the use of inexpensive
raw materials, such as triphenyl phosphine and alkali/alkaline earth metal carbonates.
(2) Easier and more economical production on a commercial scale because the reaction
conditions are simple, avoid low temperatures, and use a Wirtig reagent instead of a Wittig-
Horner type reagent.
(3) Higher yields and purity of rosuvastatin and its intermediates.
DETAILED DESCRIPTION OF THE INVENTION
One aspect of the present invention provides an improved process for the preparation of pyrimidine derivatives, e.g., intermediates of rosuvastatin, of the general Formula I
6


wherein R1 is a substituted or unsubstituted hydrocarbon group of 1 to about 10 carbon atoms including, by way of example, lower alkyl, aryl or arylalkyl, each of which may have one or more substituents; R2 and R3 are the same or different and are hydrogen or a substituted or unsubstituted hydrocarbon group of 1 to about 10 carbon atoms including by way of example, lower alkyl or aryl, each of which may be substituted with one or more substituents; R is hydrogen, a lower alkyl or a cation capable of forming a non-toxic pharmaceutically acceptable salt; each R5 are the same or different and are hydrogen or a hydrolyzable protecting group, or each R3, together with the oxygen atom to which each is bonded, form a hydrolyzable cyclic protecting group, or each R5 is bonded to the same substituent which is bonded to each oxygen atom to form a hydrolyzable protecting group; and Z is sulfur, oxygen,, sulfonyl, or imino which may be substituted by formyl, acetyl, propionyl, butyryl, isobutyryl, valeryl, isovaleryl, amino substituted by sulfonyl or alkyl sulfonyl, and sulfonyl substituted by alkyl. amino or alkylamino.
Representative lower alkyls include straight, branched, or cyclic C1 to C6 alkyls such as, for example, methyl, ethyl, n-propyl, isopropyl, cyclopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, cyclobutyl, n-pentyl, isopentyl, neopentyl, tert-pentyl, cyclopentyl, n-hexyl, and isohexyl and the like. The lower alkyls may be substituted with 1 to 3 substituents such as halogens, amino, and cyano. Suitable halogens include, but are not limited to, fluorine, chlorine, bromine, iodine and the like.
Representative aryl groups include Cg to Cn aromatic group such as, for example, phenyl, tolyl, xylyl, biphenyl, naphthyl, and the like. The aryl groups may be substituted with 1 to 3 substituents such as lower alkyls, halogens, amino, and cyano. A preferred aryl group is phenyl substituted with 1 to 3 halogens.
Representative aralkyl groups include C, to C6 lower alkyls substituted with C6 to C(2 aromatic aryl groups as defined above. Examples include benzyl, phenethyl, phenylpropyl and the like, each of which may be substituted with 1 to 3 substituents such as lower alkyls, halogens, amino, cyano, and the like.
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The term "a cation capable of forming a non-toxic pharmaceutically acceptable salt" refers to alkali metal ions, alkaline earth metal ions, ammonium ions and the like. Examples of alkali metals include lithium, sodium, potassium, and cesium. Examples of alkaline earth metals include beryllium, magnesium, and calcium. Preferred cations are sodium and calcium.
The hydrolyzable protecting groups used is preferably hydrolyzable under acidic or basic conditions. Examples of hydrolyzable protecting groups include, for example, silyl groups such as trialkylsilyl, e.g., t-butyl-dimethyl-silyl, and alkyldiarylsilyl and cyclic protecting groups such that each RD form, for example, a dioxane. In one embodiment, the hydrolyzable protecting group is wherein each R5 is bonded to the same substituent which is bonded to each oxygen atom to form a hydrolyzable protecting group, e.g.,

wherein each R is a lower alkyl.
The imino may be substituted by formyl, acetyl, propionyl, butyryl, isobutyryl, valeryl, isovaleryl, amino substituted by sulfonyl or alkylsulfonyl wherein the alkyl group has 1 to about 6 carbon atoms, and sulfonyl substituted by alkyl, amino or alkylamino. Examples of aminos substituted with alkylsulfonyls include, but are not limited to, methanesulfonyl amino, ethanesulfonyl amino, propanesulfonyl amino and the like. Examples of substituted sulfonyls as substituent include, but are not limited to, methanesulfonyl, sulfamoyl, methylsulfamoyl, N-dimethylsulfamoyl and the like.
The foregoing pyrimidine derivative can be obtained by reacting a Wittig reagent of the general Formula II;

wherein R. R , R , R , Z and X have the aforestated meanings with an aldehyde of the general Formula III
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wherein R4 and R5 have the aforestated meanings; in the presence of a base.
Suitable bases for use herein include, but are not limited to, of alkali metal carbonates, e.g., sodium carbonate, potassium carbonate, or magnesium carbonate, alkali metal hydroxides and the like and mixtures thereof. Generally, the base is present in the range of about 2.5 to about 4.5 equivalents per equivalent of the Wittig reagent. In another embodiment of the present invention, the base is present in the about 3.5 equivalents per equivalent of the Wittig reagent.
9
In a preferred embodiment, the process includes at least reacting the Wittig reagent of Formula IV:



a key intermediate in the production of rosuvastatin.
Generally, Wittig reactions are used to convert ketones or aldehydes into alkenes. In these reactions, a phosphorous ylide, R.2C=P(C6H5)3 (also called a phosphorane), is added to a ketone or aldehyde, yielding a dipolar intermediate called a betaine. The betaine intermediate of a Wittig reaction decomposes to yield an alkene and a triphenylphosphine oxide. The result is the replacement of carbonyl oxygen by the organic fragment originally bonded to the phosphorous.
In the process of the present invention, the Wittig reagent of Formula 4 acts as the ylide and reacts with the aldehyde of formula 5 (e.g., BFA) to yield a pyrimidine derivative (e.g., BEM). Examples of Wittig reagents for use in the process of the present invention include, but are not limited to, triphenyl [4-(4-fluorophenyl)-6-isopropyl-2-[methyl (methylsulfonyl)amino]pyrirnidin-5-ylmethyl] phosphonium bromide (TPPBr), tributyl [4-(4-fluorophenyl)-6-isopropyl-2-[methyl(methylsulfonyl)amino]pyrimidin-5-ylmethyl] phosphonium bromide (TBPBr) and the like.
Generally, TPPBr may be prepared by reacting an alcohol of Formula VII:

10


The bromide intermediate is then reacted with triphenyl phosphine to yield TPPBr. The quality and yield of TPPBr depends on how the reagents are added. To obtain the best yields, the bromide intermediate of Formula 7 was added to preheated triphenyl phosphine (P(Ph)3) preheated to a temperature in the range of from about 80°C to about 100°C. This results in high quality production of TPPBr.
Generally, TBPBr may be prepared by reacting an alcohol of Formula VII:

with phosphorous tribromide (PBr3) to form a bromide intermediate of Formula VIII:
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The bromide intermediate is then reacted with tributylphosphine to yield TBPBr. A preferred aldehyde for use herein is of the general formula

wherein R4 has the aforestated meaning and R5 is a hydrolysable protecting group such as a lower alkyl as defined hereinabove.
The reaction of the Wittig reagent and aldehyde may be carried out at a temperature ordinarily ranging from about 40°C to about 90°C, and more preferably at a temperature ranging from about 60°C to about 80°C. In one embodiment, the aldehyde can be added to the Wittig reagent in an excess amount. In another embodiment, the aldehyde is present in the range of about 1.0 to about 1.3 equivalents per equivalent of the Wittig reagent. The time period for the reaction can range from about 30 minutes to about 3 hours.
If desired, the reaction of the Wittig reagent and aldehyde can be carried out in an aprotic solvent. Suitable aprotic solvents for use in the processes of the present invention include, but are not limited to, those selected from dimethyl sulfoxide (DMSO). dimethylformamide (DMF), dimethylacetamide (DMA) and the like and mixtures thereof. The solvent can be present in a ratio of Wittig reagent to solvent ordinarily ranging from about 1:3 w/v to about 1:5 w/v.
Following the formation of the Wittig reaction product, the Wittig reaction product can be hydrolyzed by techniques known in the art to provide a pyrimidine derivative such as rosuvastatin as set forth below in Formula IX:
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wherein R, R1, R2, R3, R4 and Z have the aforestated meanings or a racemic mixture, an enantiomer, a diastereoisomer or a mixture thereof. For example, the Wittig reaction product can be hydrolyzed with a suitable hydrolyzable acid such as, for example, a dilute (about 0.02N to about 1.25N) aqueous mineral acid such as hydrochloric acid at a temperature ranging from about 20°C to about 60°C, and preferably at a temperature ranging from about 40°C to about 45°C, for a time period ranging from about 3 to about 6 hours.
As one skilled in the art will readily appreciate, the hydrolyzed pyrimidine derivative of Formula IX can then be converted to a salt such as sodium or calcium salt using techniques known in the art. For example, the hydrolyzed pyrimidine derivative can be converted to a salt using IN sodium hydroxide solution at room temperature for about 4 to about 5 hours in a suitable solvent medium such as an alcohol solvent.
In another embodiment of the present invention, the pyrimidine derivative of Formula I can be converted to a pharmaceutically acceptable salt. In one embodiment, the pyrimidine derivative of Formula I can be converted to a pharmaceutically acceptable salt by contacting the pyrimidine derivative with a sufficient amount of a source of calcium, e.g., calcium chloride, calcium acetate and the like,
A "sufficient amount" as used herein refers to the amount of the source of calcium that substantially converts the pyrimidine derivative to the corresponding calcium salt. By "substantially converts" as used herein is meant an amount such that greater than about 50% (molar basis), preferably greater than about 70%, more preferably greater than about 90% and most preferably greater than about 95% of the pyrimidine derivative is converted to the corresponding calcium salt.
In another embodiment of the present invention, the pyrimidine derivative of Formula I can be converted to a pharmaceutically acceptable salt by first contacting the pyrimidine derivative with an amine source such as an amine of the formula R6R7NH2, wherein
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R6 and R7 are the same or different and can be an alkyl of from 1 to 6 carbon atoms in the presence of at least a suitable hydrolyzable acid such as a dilute (e.g.. about 0.02N to about 1.25N) aqueous mineral acid such as hydrochloric acid to produce an alkylammonium salt of the pyrimidine derivative of the general Formula X:

wherein R. R1, R2, R3, and Z have the aforestated meanings. The reaction can be carried out in at a temperature ranging from about -5°C to about 30 °C for a time period ranging from about 3 hours to about 5 hours. Generally, the amine source will be present in an amount ranging from about 1.6 to about 1.8 equivalents (relative to the pyrimidine derivative). Next, the alkylammonium salt of the pyrimidine derivative of Formula X will be converted to a pharmaceutically acceptable salt by contacting the contacting the alkylammonium salt of the pyrimidine derivative with a sufficient amount of a source of calcium, e.g., calcium chloride.
In another embodiment of the present invention, an improved process for the preparation of rosuvastatin is provided including the steps of (a) reacting a Wittig reagent (Formula 1) with BFA (Formula 2) to form BEM (Formula 3); (b) treating the BEM (Formula 3) with hydrochloric acid, sodium hydroxide, sodium chloride, and methylamine to form the methyl ammonium salt of rosuvastatin (4); and (c) reacting the methylammonium salt of rosuvastatin (4) with calcium chloride to form the calcium salt of rosuvastatin (5). This process is generally depicted in Scheme 1:
14


15

This process includes the steps of (a) reacting an alcohol of Formula 6 with PBr3 to form a bromide intermediate (7); (b) reacting the bromide intermediate (7) with P(Ph)3 to form the Wittig reagent TPPBr (1a); (c) reacting TPPBr (la) with BFA (2) to form BEM (3); (d) treating the BEM (3) with hydrochloric acid, sodium hydroxide, sodium chloride, and dimethylamine to form the methylammonium salt of rosuvastatin (4); and (e) reacting the methylammonium salt of rosuvastatin (4) with calcium chloride to form the calcium salt of rosuvastatin (5).
In still yet another embodiment of present invention, rosuvastatin can be prepared as generally depicted in Scheme 3:

This process comprises the steps of: (a) reacting an alcohol of Formula 6 with PBr3 to form a bromide intermediate (7); (b) reacting the bromide intermediate (7) with P(n-Bu)3 to form the Wittig reagent TBPBr (Ib); (c) reacting TBPBr (Ib) and BFA (2) to form BEM (3); and (d) treating the BEM (3) with hydrochloric acid, sodium hydroxide, and calcium acetate to form the calcium salt of rosuvastatin (5).
The following examples are provided to enable one skilled in the art to practice the invention and are merely illustrative of the invention. The examples should not be read as limiting the scope of the invention as defined in the claims.
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EXAMPLE 1 Preparation of TPPBr
Into a 2L 4-necked round bottom flask, [4-(4-fluorophenyl)-6-isopropyl-2-(N-methyl-N-methyl sulfonylarnino)pyrimidin-5-yl]methanol (lOOg), toluene (800ml) and acetonitrile (400ml) were added at a temperature in the range of from about 25°C to about 30°C under stirring. The reaction mixture was cooled to a temperature of about 15°C. Phosphorous tribromide (35g) was slowly added over about 30 minutes at a temperature in the range of from about 15°C to about 20°C. After about 30 minutes, the reaction mixture was added to water (1000ml) while maintaining the temperature in the range of from about 15°C to about 20°C. The reaction mixture was stirred for about 5 minutes and the organic layer was separated.
The organic layer was washed with a 10% solution of sodium bicarbonate (500ml), followed by water (500ml). The organic layer was concentrated at a temperature in the range of from about 60°C to about 70°C under reduced pressure until a distillate (about 800ml) was collected. The residue was cooled to a temperature of about 60°C. A preheated (ranging from about 80°C to about 100°C) mixture of triphenyl phosphine (72gm) in toluene (500ml) was added to the residue. The reaction mixture was heated to a temperature of about 110°C and maintained for about 3 to about 4 hours. After completion of the reaction as determined by TLC, the reaction mixture was cooled to a temperature in the range of from about 20°C to about 25°C. The precipitated product was filtered, washed with toluene (250ml), and dried in a vacuum oven at a temperature of below about 60°C until the moisture content was about 1%. The dried product appears as an off-white crystalline solid weighing about 170g to about 180g. The yield was about 88% to about 94%, having a melting point (m.p.) of 238-242°C, and a purity of -99.5% as determined by HPLC.
The IR (KBr) spectrum shows the absorption bands at 3100 cm"1 (C-H str), 2900 (C-H sir), 1606 (-C=C-str), 1150 (SO2 str). The LH-NMR (DMSO-d6) shows 57.89 [m,2H,Ar-H], 7.6-7.4 [m,15H,P(C6H5)3], 7.12 [m,2H,Ar-H], 5.05 [dslH,CH2P], 3.55-3.75 [2xs,6H,NCH3,S02CH33, 2.95 [hept,lH,CH(CH3)2], 0.89 [d,6H,CH(CH3)2]. The CI mass shows m/z 600 (base peak). The elemental analysis calculated %C 60.18; %H 5.05; %N 6.19. Observed %C 60.05; %H 5.0, %N 6.0.
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EXAMPLE 2 Preparation of TBPBr
Into a 2L 4-necked round bottom flask, [4-(4-fluorophenyl)-6-isopropyl-2-(N-methyl-N-methyl sulfonyl amino) pyrimidin-5-yI]methanol (lOOg), toluene (800ml) and acetonitrile (400ml) were added at a temperature in the range of about 25°C to about 30°C under stirring. The reaction mixture was cooled to a temperature of about 15°C, and phosphorous tribromide (35g) was added at a temperature in the range of about 15°C to about 20°C slowly over about 30 minutes. Next, the reaction mixture was added to water (1000ml) over about 30 minutes, while maintaining the temperature in a range of from about 15°C to about 20°C. The reaction mixture was stirred for 5 minutes at a temperature in the range of from about 15°C to about 20°C. The organic layer was separated and washed with 10% solution of sodium bicarbonate (500ml) followed by water (500ml). The organic layer was concentrated at a temperature in the range of from about 60°C to about 70°C under reduced pressure. The white solid obtained was then added to toluene (300ml) at a temperature of about 25°C. Tri-n-butyl phosphine was then slowly added. After about 20 to about 25 minutes, a bright white solid falls out and is filtered and washed with toluene (lOOmL). The solid was then dried in an oven at a temperature below about 50°C until the moisture content was about 1%. The dried solid appeared as a white crystalline solid weighing about 29g to about 29.5g. The yield was about 97% to about 99%, having a mp 180-182°C, and a purity greater than 99% as determined by HPLC.
The IR (KBr) spectrum shows the absorption bands at cm"1 2962 (C-H str), 1604 (-OC-str), 1155 (S02 str). The ]H-NMR (DMSO-d6) shows 87.8-7.9[dd,2H,Ar-H], 7.4-7.5[t,2H,Ar-H], 4.0[d;2H,CH2P]33.4-3.5[2xs,6H,NCH3)S02CH3], 3.41[m,lH,CH(CH3)2], 1.9-2.0[t,6H,P-(CH2)])1.10-1.29[m;18H,-(CH2)3-]0.81[t,9H,(CH2-CH3)3]. The CI mass shows m/z 538.4 (base peak). The elemental analysis calculated %C 54.36; %H 7.49; %N 6.79; Observed %C 54.30; %H 7.415%N 6.72.
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EXAMPLE 3 Preparation of BEM
Into a 2L 4-necked round bottom flask, DMSO (600ml), potassium carbonate (104g), TPPBr (170g) of Example 1 and BFA (65g) were added at a temperature in the range of from about 25°C to about 35°C under stirring. The reaction mixture was heated to a temperature in the range of from about 70°C to about 75°C for about 2 hours. After completion of the reaction as determined by TLC, the reaction mixture was cooled to a temperature in the range of from about 25°C to about 35°C. Toluene (1000ml) was added for dilution of the reaction mixture under stirring for about 30 minutes at a temperature in the range of from about 25°C to about 35°C. The reaction mixture was filtered, and the insoluble cake was separated. The organic layer was added to water (1000ml) under stirring at a temperature in the range of from about 25°C to about 35°C and maintained for about 30 minutes.
The organic layer was separated and washed twice with water (2x1000ml). The organic layer was distilled in rotavapor bath at a temperature in the range of from about 50°C to about 70°C under in a vacuum. After distillation, methanol (750ml) was added to the residue at a temperature in the range of from about 55°C to about 60°C and maintained for about 30 minutes. The reaction mixture was brought to a temperature in the range of from about 25°C to about 30°C by slowly circulating water. During this time, a product precipitated out. The precipitated mass was further cooled to a temperature of about 10°C for about 30 minutes and then filtered. The cake was washed with prechilled methanol (100ml at a temperature of about 10°C). The product was dried in an oven at a temperature of about 55°C at a temperature of about 10°C until the moisture content was about 1%. The dried product appeared as an off-white crystalline solid weighing about 108g to about 110g. The yield was about 75% to about 76%, having a m.p. of 150-154°C.
The 1R (KBr) spectrum shows the absorption bands at 3100 cm"1 (C-H str), 2900 (C-H str), 1720 (-COO str), 1605 (-OC-str), 1150 (S02str). The HNMR (CDC13) TMS as internal standard shows the following signals 5 7.65 [m,2H,Ar-H], 7,09 [m,2H,Ar-H], 6.52 [dd,lH,ArCH=CH], 5.47 [dd,!H,ArCH=CH], 3.57, 3.50 [2xs,6HsNCH3sSO2CH3], 3.38 [hept,lH,Ar-CHMe2], 2.45-2.30 [2xdd,2H,CH2C02 t-Bu], 1.55, 7.3 [dt,dd,2H,acetonide;CH2], 1,50, 1.40 [2xs,6H,acetonide C(CH3)2], 1.45 [s,9H,C02C(CH3)3], 1.27 [dd,6H,ArCH(CH3)2].
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The CI mass shows m/z 578 (base peak). The elemental analysis shows calculated %C 60.29; %H 6.98; %N 7.27; observed %C 60.12; %H 6.89; %N 7.17.
EXAMPLE 4 Preparation of BEM
In a 250mL 4-necked round bottom flask, DMSO (150ml), potassium carbonate (33.54g): TBPBr (50g) of Example 2 and BFA (20.87g) were added at a temperature in the range of about 25°C to about 35°C under stirring. The reaction mixture was heated to a temperature of about 70°C to about 75°C for about 5 to about 7 hours. After the completion of the reaction as determined by TLC, the reaction mixture was cooled to a temperature in the range of about 25°C to about 35°C. Toluene (250ml) was added for dilution of the reaction mixture and stirred for about 30 minutes. The organic layer was added to water (100ml) under stirring and maintained for 30 minutes. The organic layer was separated and washed with water (2x100 ml) in the same manner as described above. The organic layer was distilled in Rota vapor bath at temperature in the range of from about 50°C to about 60°C under vacuum. After distillation. IPA (100ml) was added to the residue at a temperature of about 55°C to about 60°C and the mixture was maintained for about 30 minutes. The reaction mixture was brought to a temperature in the range of from about 25°C to about 30°C by circulating room temperature water slowly. In this period, the product precipitates and-the precipitated mass was further cooled to a temperature of about 10°C for about 30 minutes, and then filtered. The cake was washed with prechilled (at a temperature of about 10°C) IPA (50 ml). The product was dried in an oven at a temperature of about 55°C until the moisture content was about 1%. The dried product appeared as an off-white crystalline solid weighing about 44g to about 45g. The yield was about 88% to about 90%. and having a m.p. of 150-154°C.
The IR (KBr) spectrum shows the absorption bands at 3100 cm"1 (C-H str), 2900 (C-H str), 1720 (-COO str), 1605 (-OC-str); 1150 (S02str). The 'HNMR (CDC13) TMS as internal standard shows the following signals 6 7.65 [m,2H,Ar-H], 7.09 [m,2H,Ar-H], 6.52 [dd,lH,ArCH-CH], 5.47 [dd,lH,ArCH=CH], 3.57, 3.50 [2xsf6H,NCH3;SO2CH3], 3.38 [hept,lH,Ar-CHMe2], 2.45-2.30 [2xdd,2H,CH2CO2t-Bu], 1.55, 7.3 [dt,dd,2H,acetonide,CH2], 1.50, 1.40 [2xs,6H,acetonideC(CH3)2], 1.45 [s,9H,C02C(CH3)3], 1.27 [dd,6H;ArCH(CH3)2]. The
20

CI mass shows m/z 578 (base peak). The elemental analysis shows calculated %C 60.29; %H 6.98; %N 7.26; observed %C 60.17; %H 6.93; %N 7.21.
EXAMPLE 5 Preparation of Rosuvastatin Methylammonium salt
Into a 2L 4-necked round bottom flask, BEM (lOOg) of Example 4 and acetonitrile (700ml) were added and stirred under an inert atmosphere at about 40°C. Next, 0.02M hydrochloric acid (190ml) was added to the reaction mixture over about 30 minutes while maintaining the temperature in the range of from about 35°C to about 40°C. The mixture was stirred at a temperature of about 40°C for about 3 hours. After completion of the reaction as determined by TLC. the reaction mixture was cooled to a temperature of about 25°C. 1.0 M sodium hydroxide solution (190ml) was added to the reaction mixture under stirring and the mixture was stirred for an additional one hour. After completion of the reaction as determined by TLC, sodium chloride (94g) was added and the mixture was cooled to a temperature of about -5°C for about 1 hour, A solution of 1M HC1 (190ml) and sodium chloride (48g) were added at a temperature of about -5°C to achieve a pH in the range of from about 3.4 to about 4. The mixture was stirred for about 5 to about 10 minutes. The mixture was allowed to settle for about 10 minutes at a temperature of about -5°C, resulting in two layers. The bottom layer was separated and discarded. Acetonitrile (1.3L) was added to the remaining solution at a temperature of about -5°C. The mixture was filtered through a hyflow bed. A 40% methylamine solution (24ml) was added at a temperature of about -5°C. The mixture was then warmed to a temperature of about 30°C over about 40 minutes and maintained for about 90 minutes. The mixture was then cooled to a temperature of about 0°C over about 40 minutes and maintained for about 90 minutes. The resulting solid was filtered and washed twice with acetonitrile (2x200ml). The isolated solid is the methylamine salt of the rosuvastatin. It was dried at a temperature in the range of from about 35°C to about 45°C under a vacuum. The dried product appeared as an off-white crystalline solid weighing about 65g to about 75g. The yield was about 71% to about 84%, having a purity of 99% (HPLC) and a m.p. of 154-160°C.
The 1R (KBr) spectrum shows the absorption bands at 3540-3400 cm"' (O-H, N-H str), 3100 (C-H str), 2900 (C-H str), 1660(-COO- str), 1605 (-C=C- str), 1150 (SO2str). The !H-
21

NMR (CDCI3) TMS as internal standard shows the following signals 6 7.7 (2H,t)s 7.3 (2H,t), 6.5 (lH,d), 5.5 (lH,dd), 4.2(lH,m)s 3.8(lH,m), 3.4(6H,s), 3.5 (3H,s), 1.9-2.1(2H,dd), 1.3-1.5 (2H,m), 1.2 (6H,d). The CI Mass shows m/z 482 (base peak). The elemental analysis shows calculated %C 53.89; %H 6.49; %N 10.93, and observed %C 53.72; %H 6.38; %N 10.80.
EXAMPLE 6 Preparation of Rosuvastatin Calcium Salt
Into a 2L 4-necked round bottom flask, rosuvastatin methyl ammonium salt (lOOg) of Example 5 and demineralized water (500m) were added at a temperature of about 20°C under stirring. Aqueous sodium hydroxide solution (8% w/w, 90.6ml) was added at a temperature of about 20°C under stirring for about 1 hour. The mixture was filtered and concentrated at a temperature below about 40°C under reduced pressure. A distillate (about 400ml) was collected. Demineralized water (400ml) was added and the mixture was again concentrated under reduced pressure at a temperature of about 40°C. A distillate (about 400ml) was collected, Demineralized water (500ml) was added to the residue, followed by dropwise addition of a solution of calcium chloride dihydrate (17.16g) in demineralized water (103ml) at a temperature of about 20°C. The mixture was stirred for about 45 minutes at and a solid was precipitated. The solid was filtered, washed with water (600ml), and dried under vacuum until the moisture content was about 3.5% to about 5% at a temperature of about 40°C. The dried product was the calcium salt of rosuvastatin and appeared as an off-white crystalline solid weighing about 80g to about 85g. The yield was about 79% to about 84%, having a purity of 99% (HPLC).
The specific optical rotation a D 20 = +15 -16° (C=0.5% in MeOH). The DSC shows two broad endotherms. The first endotherm shows a peak temperature of 85.76°C (onset temp 63.6°C corresponding to the water). The second endotherm shows a peak temperature of 225 °C (onset temperature 198°C). The IR (KBr) spectrum shows the absorption bands at 3500-3400 cm'1 (0-H str), 3100 (C-H str), 2900 (C-H str), 1704 (-COO - str), 1605 (-OC-str), 1150 (SO2str). The 'HNMR (CDC13) TMS as internal standard shows the following signals 6 7.7 (2H,t), 7.3 (2H,t), 6.5 (lH,d), 5.5 (!H,dd), 4.2(lH,m), 3.8(lH,m), 3.5(3H,s), 1.9-2.1(2H,dd), 1.3-1.5 (2H,m), 1.2 (6H,d). The CI Mass shows m/z 482 (base peak). The elemental analysis shows calculated %C 50.76; %H 5.23; %N 8.07; and observed %C50.58; %H 5.15; %N 7.8.
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It will be understood that various modifications may be made to the embodiments disclosed herein. Therefore the above description should not be construed as limiting, but merely as exemplifications of preferred embodiments. For example, the functions described above and implemented as the best mode for operating the present invention are for illustration purposes only. Other arrangements and methods may be implemented by those skilled in the art without departing from the scope and spirit of this invention. Moreover, those skilled in the art will envision other modifications within the scope and spirit of the claims appended hereto.
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WHAT IS CLAIMED IS:
1. A process for the preparation of pyrimidine derivatives comprising reacting a Wittig reagent of the general formula
wherein R is an alkyl of from 1 to 10 carbon atoms, aryl or arylalkyl, Rl is a substituted or
unsubstituted hydrocarbon group, R and R are the same or different and are hydrogen or a substituted or unsubstituted hydrocarbon group; Z is sulfur, oxygen, , sulfonyl, or imino which may be substituted by formyl, acetyl, propionyl, butyryl. isobutyryl, valeryl, isovaleryl, amino substituted by sulfonyl or alkyl sulfonyl. and sulfonyl substituted by alkyl, amino or alkylamino and X is a halogen; with an aldehyde of the general formula

wherein R4 is hydrogen, a lower alkyl or a cation capable of forming a non-toxic pharmaceutically acceptable salt and each R5 are the same or different and are hydrogen or a hydrolyzable protecting group, or each R5, together with the oxygen atom to which each is bonded, form a hydrolyzable cyclic protecting group, or each R5 is bonded to the same substituent which is bonded to each oxygen atom to form a hydrolizable protecting group; in the presence of a base to provide a pyrimidine derivative of the general formula

24

wherein R, R!, R2, R3, R4, R5, X and Z have the aforestated meanings or a racemic mixture, an enantiomer, a diastereoisomer or a mixture thereof.
2. The process of Claim 1, wherein R1 is a substituted or unsubstituted hydrocarbon
group of 1 to about 10 carbon atoms, R2 and R3 are the same or different and are a substituted or
unsubstituted hydrocarbon group of 1 to about 10 carbon atoms and R4 is a lower alkyl of 1 to 6
carbon atoms.
3. The process of Claim 1, wherein R1 is selected from the group consisting of lower
alkyl which may have 1 to 3 substitutents independently selected from the group consisting of
halogen, amino, and cyano, a C& to Cn aromatic group which may have 1 to 3 substituents
independently selected from the group consisting of lower alkyl, halogen, amino, and cyano, and
Ci to Cf, lower alkyl substituted by C& to Ci2 aromatic group which may have 1 to 3 substituents
-i
independently selected from the group consisting of lower alkyl, halogen, amino, and cyano; R is a lower alkyl which may have 1 to 3 substituents independently selected from the group consisting of halogen, amino, and cyano, or a Cg to C\2 aromatic group which may have 1 to 3 substituents independently selected from the group consisting of lower alkyl, halogen, amino, and cyano; R3 is a lower alkyl which may have 1 to 3 substituents independently selected from the group consisting of halogen, amino, and cyano, R4 is hydrogen or a lower alkyl which may have 1 to 3 substituents independently selected from the group consisting of halogen, amino, and cyano.
4. The process of Claim 1, wherein R1 is a lower alkyl of 1 to 6 carbon atoms; R2 is a Ce
to Ci2 aromatic group which may have 1 to 3 substituents independently selected from the group
consisting of lower alkyl, halogen, amino, and cyano; R3 is a lower alkyl of 1 to 6 carbon atoms,
R is a lower alkyl of 1 to 6 carbon atoms.
5. The process of Claim 1, wherein Z is an amino substituted by sulfonyl or alkylsulfonyl
wherein the alkyl group contains from 1 to 6 carbon atoms.
6. The process of Claim 1, wherein Z is methanesulfonyl amino.
25

7. The process of Claim 1, wherein the Wittig reagent is triphenyl [4-(4-fluorophenyl)-6-isopropyl-2-[methyl (methylsulfonyl)amino]pyrimidin-5-ylrnethyl] phosphonium bromide or tributyl [4-(4-fluorophenyl)-6-isopropyl-2-[methyl(methyIsulfonyl)amino]pyrimidin-5-ylmethyl] phosphonium bromide and the aldehyde is of the general formula

wherein R has the aforestated meaning and R is a hydrolysable protecting group.
8. The process of Claim I, wherein the base is selected from the group consisting of
alkali metal carbonates, alkali metal hydroxides and mixtures thereof.
9. The process of Claim 1, wherein the base is an alkali metal carbonate is selected from
the group consisting of sodium carbonate, potassium carbonate, magnesium carbonate and
mixtures thereof.
10. The process of Claim 1, wherein the reaction is carried out at a temperature of about
40°C to about 90°C.
11. The process of Claim 1, wherein the reaction is carried out at a temperature of about
60°C to about 80°C.
12. The process of Claim 1, wherein the base is present from about 2.5 to about 4.5
equivalents per equivalent of the Wittig reagent.
13. The process of Claim I, wherein the base is present from about 3.5 equivalents per
equivalent of the Wittig reagent.
26

14. The process of Claim 1, wherein the aldehyde is added to the Wittig reagent in an
excess amount.
15. The process of Claim I, wherein the aldehyde is present from about 1 to about 1.3
equivalents per equivalent of the Wittig reagent.
16. The process of Claim 1, further comprising an aprotic solvent.
17. The process of Claim 16, wherein the aprotic solvent is selected from the group
consisting of dimethyl sulfoxide, dimethylformamide, dimethylacetamide and mixtures thereof.
18. The process of Claim 16 wherein the solvent is present in a ratio of Wittig reagent to
solvent of from about 1:3 w/v to about 1:5 w/v.
19. The process of Claim 1, further comprising hydrolyzing the pyrimidine derivative to
produce the hydrolyzed pyrimidine derivative of the general formula:

wherein R, R , R , R , R4 and Z have the aforestated meanings or a racemic mixture, an enantiomer, a diastereoisomer or a mixture thereof.
20. The process of Claim 19. wherein the step of hydrolyzing the pyrimidine derivative
comprises contacting the pyrimidine derivative with a dilute acid.
21. The process of Claim 20. wherein the dilute acid is hydrochloric acid.
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22. The process of Claim 19, wherein the hydrolyzed pyrimidine derivative is converted
to a salt of the pyrimidine derivative or a pharmaceutically acceptable salt of the pyrimidine
derivative.
23. The process of Claim 19, wherein the hydrolyzed pyrimidine derivative is converted
to a calcium salt of the pyrimidine derivative.
24. The process of Claim 19. wherein the hydrolyzed pyrimidine derivative thus
obtained is purified.
25. The process of Claim 1, wherein the product pyrimidine derivative is converted to a
salt of the pyrimidine derivative or a pharmaceutically acceptable salt of the pyrimidine
derivative.
26. The process of Claim 1. wherein the product pyrimidine derivative is converted to a
calcium salt of the pyrimidine derivative.
27. The process of Claim 1, wherein the product pyrimidine derivative is contacted with
a sufficient amount of a source of calcium in the presence of a dilute acid to provide a calcium
salt of the pyrimidine derivative.
28. The process of Claim 1, wherein the product pyrimidine derivative is contacted with
a sufficient amount of a calcium acetate in the presence of a dilute acid to provide a calcium salt
of the pyrimidine derivative.
28

29. The process of Claim 1, further comprising contacting the product pyrimidine derivative with an amine of the general formula R6R7NH2 wherein R6 and R7 are the same or different and can be an alkyl of from 1 to 6 carbon atoms in the presence of a dilute acid to produce an alkylammonium salt of the pyrimidine derivative of the general formula;

wherein R, R1, R2, R3, and Z have the aforestated meanings.
30. The process of Claim 30, wherein the dilute acid is hydrochloric acid.
31. The process of Claim 29, wherein the alkyl ammonium salt of the pyrimidine
derivative is converted to a pharmaceutically acceptable salt.
32. The process of Claim 31, wherein the pharmaceutical ly acceptable salt is a calcium
salt of the pyrimidine derivative.
33. The process of Claim 29, further comprising contacting the alkyl ammonium salt of
the pyrimidine derivative with a sufficient amount of a source of calcium to provide a calcium
salt of the pyrimidine derivative.
34. The process of Claim 29, further comprising contacting the alkyl ammonium salt of
the pyrimidine derivative with a sufficient amount of calcium chloride to provide a calcium salt
of the pyrimidine derivative.
35. The process of Claim ], further comprising contacting the product pyrimidine
derivative with an amine of the general formula R6R7NH2 wherein R6 and R7 are the same or
29

different and can be an alkyl of from 1 to 6 carbon atoms in the presence of a dilute acid to produce an alkylammonium salt of the pyrimidine derivative of the general formula:

wherein R, R , R , R , and Z have the aforestated meanings; and contacting the alkyf ammonium salt of the pyrimidine derivative with a sufficient amount of calcium chloride to provide a calcium salt of the pyrimidine derivative.
36. A process for the preparation of BEM comprising the reacting a Wittig reagent of Formula 1:

wherein R is the same or different and can be an alkyl of from 1 to 10 carbon atoms, aryl or an arylalkyl and X is a halogen, with tert-butyl-2-[(4R,6S)-6-formyl-2,2-dimethyl-l,3-dioxan-4-yl]acetate (BFA) of the general formula

in the presence of a base.
30

37. The process of Claim 36, wherein the Wittig reagent is triphenyl [4-(4-fluorophenyl)-
6-isopropyl-2-[methyl (methylsulfonyl)amino]pyrimidin-5-ylrnethyl] phosphonium bromide.
38. The process of Claim 36, wherein the Wittig reagent is tributyl [4-(4-fluorophenyl)-
6-isopropyl-2-[methyl(methylsulfonyI)amino]pyrimidin-5-ylmethyl] phosphonium bromide.
39. The process of Claim 36, wherein the reaction is carried out at a temperature of about
40°C to about 90°C.
40. The process of Claim 36, wherein the reaction is carried out at a temperature of about
60°C to about 80°C.

41. The process of Claim 36, wherein the base is present from about 2.5 to about 4.5
equivalents per equivalent of the Wittig reagent,
42. The process of Claim 36, wherein the base is present in about 3.5 equivalents per
equivalent of the Wittig reagent.
43. The process of Claim 36, wherein BFA is present from about 1.0 to about 1.3
equivalents per equivalent of the Wittig reagent.
49. The process of Claim 36, wherein the base is selected from the group consisting
of sodium carbonate, potassium carbonate, magnesium carbonate and mixtures thereof.
50. The process of Claim 36, wherein the reaction takes place in the presence of a
solvent.
51. The process of Claim 50, wherein the solvent is selected from the group consisting of
dimethyl sulfoxide, dimethylformamide, dimethylacetamide and mixtures thereof.
52. The process of Claim 36, further comprising converting BEM to a calcium salt of
rosuvastatin.
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53. The process of Claim 52, wherein the step of converting comprises contacting BEM
with a sufficient amount of a source of calcium in the presence of a dilute acid to provide the
calcium salt of rosuvastatin.
54. The process of Claim 52, wherein the step of converting comprises contacting BEM
with a sufficient amount of calcium acetate in the presence of a dilute acid to provide the calcium
salt of rosuvastatin.
55. A process for the preparation of a rosuvastatin salt comprising the steps of:
(a) reacting a Wittig reagent of Formula 1:

wherein R is an alkyl of from 1 to 10 carbon atoms, aryl or arylalkyl and X is a halogen, with BFA in the presence of a base to form BEM; and
(b) converting BEM to a salt of rosuvastatin.
56. The process of Claim 55, wherein the salt is the calcium salt of rosuvastatin.
32


57. A compound of the general formula
wherein R is an alkyl of from 1 to 10 carbon atoms, aryl or arylalkyl, R' is a substituted or
unsubstituted hydrocarbon group, R and R are the same or different and are hydrogen or a substituted or unsubstituted hydrocarbon group; Z is sulfur, oxygen, sulfonyl, or imino which may be substituted by formyl, acetyl, propionyl, butyryl, isobutyryl, valeryl, isovaleryl, amino substituted by sulfonyl or alkylsulfonyl, and sulfonyl substituted by alkyl, amino or alkylamino and X is a halogen.

wherein R and X have the aforestated meanings.
Dated this third (03rd) day of December, 2004
33
58. The compound of Claim 57, which is of the formula (1):