PROCESSES FOR REDUCING IMPURITIES IN LACOSAMIDE
Field of the Invention
The present invention relates to processes for reducing impurities in lacosamide
during preparation of lacosamide. The invention provides processes for minimizing or
removing impurities such as (2R)-2-(acetylamino)-3-(benzylamino)-3-oxopropyl acetate
of Formula II or (2R)-2-propanoylamino-N-benzyl-3-methoxypropionamide of Formula
III in lacosamide.
Background of the Invention
Lacosamide (SPM 927, also referred to as harkoseride or ADD 234037), is
chemically (R)-2-acetamido-N-benzyl-3-methoxypropionamide and represented by
Formula I . It has been reported to be effective for the treatment of pain, epilepsy,
fibromyalgia syndrome, osteoarthritis and migraine. It is also known to be useful for the
treatment of CNS disorders in humans.
FORMULA I
Lacosamide is available in the United States market as solution and tablet dosage
forms with proprietary name of Vimpat®. The tablets are indicated as adjunctive therapy
in the treatment of partial-onset seizures in patients with epilepsy aged 17 years and older.
The solution (injection) dosage form is useful when oral administration is temporarily not
feasible.
Lacosamide and its methods of preparation are disclosed in U.S. Reissue Patent
No. 38,551 (hereinafter referred to as the '551 patent). This provides three general
methods for the preparation of lacosamide. The first two methods do not involve
protection of active groups in intermediate compounds (such as amino, hydroxy and
carboxylic acid groups). The third method involves protection of amino group present in
D-serine with carbobenzoxy chloride (Cbz-Cl), subsequent O-methylation at hydroxy
group followed by benzylamination at carboxylic (-COOH) group and finally removal of
the 'Cbz' group followed by acetylation produces lacosamide.
An alternative method for the preparation of lacosamide is disclosed in WO
2006/037574 (hereinafter referred to as the '574 application) that comprises Omethylation
of N-Boc-protected-D-serine ('Boc' refers to i-butoxycarbonyl) directly in
one step by avoiding simultaneous formation of methyl ester moiety.
U.S. Publication No. 2009/0143472 (hereinafter referred to as U.S. '472
publication) and IP.com publication (IPCOM000181080D) describes processes for the
preparation of lacosamide using trityl or pthalamide protected intermediates, respectively.
General methods known to date for the preparation of lacosamide can be
represented by following synthetic scheme (Figure 1, wherein R is nitrogen protecting
group, e.g., Cbz, Boc, trityl, pthalamide etc.).
The inventors of the present invention observed that when protecting the amino
group of D-serine, some percentage of hydroxyl groups (of D-serine) were also protected,
thereby forming the protected or blocked intermediate compound(s). During the
deprotection step (i.e., penultimate stage-Figure I), the blocked hydroxyl groups became
deprotected and therefore in the last step, (2R)-2-(acetylamino)-3-(benzylamino)-3-
oxopropyl acetate [hereinafter, referred as "Impurity-A"] of Formula II is obtained as an
impurity along with lacosamide.
FORMULA II
The present inventors also observed that commercially available acetic anhydride
contained some percentage of propanoic anhydride. As a result, during the last acetylation
step (figure I), (2R)-2-propanoylamino-N-benzyl-3-methoxypropionamide of Formula III
(hereinafter referred as "Impurity-B") is obtained as an impurity along with lacosamide.
FORMULA III
Therefore, lacosamide produced using known methods contains Impurity-A and/or
Impurity-B. Thus, there is a need for simple and cost effective process for the preparation
of lacosamide that eliminates or reduces the chances of having said impurities in
lacosamide.
Summary of the Invention
The present invention provides a process for reducing or eliminating the impurities
in lacosamide.
The present invention provides a process for reducing the content of "Impurity-A"
in lacosamide during the preparation of lacosamide, therein comprising a step for treating
D-serine with the protecting reagent, wherein, number of moles for the protecting reagent
are less then the number of moles of D-serine.
The present invention also provides a process for reducing the content of
"Impurity-B" in lacosamide during the preparation of lacosamide, therein comprises a step
of acetylating the compound of Formula IV in aqueous medium.
NH2
FORMULA IV
The present invention also provides lacosamide with an improved impurity profile.
The present invention also provides lacosamide substantially free of Impurity-A.
The present invention also provides lacosamide substantially free of Impurity-B.
The present invention also provides processes for preparation of lacosamide
wherein lacosamide is substantially free of "Impurity-A" and "Impurity-B".
Brief Description of the Figure
1: General methods for preparation of lacosamide.
Description of Terms
The term "lacosamide", as used herein, refers to R-enantiomeric form of 2-
acetamido-N-benzyl- 3-methoxypropionamide .
The term "0-methylation", as used herein, refers to attachment of methyl group to
the main chain through an oxygen bridge. Alternatively the "0-methylation" is a process
of converting -OH group into -OMe group in a given chemical compound.
The term "benzylamination", as used herein, refers to attaching -NH-CH2-C6H5
group in a given compound in such a way that the terminal -NH moiety can form amide
group.
The term "protecting reagent", as used herein, can be selected from the group
comprising carbobenzyloxy chloride, tert.-butoxycarbonyl chloride, trityl chloride,
pthaloyl chloride, and like reagents. Therefore, the protecting groups are carbobenzyloxy,
tert.-butoxycarbonyl, trityl, pthaloyl, and the like.
The term "deprotection", as used herein, refers to removal of protecting group
from the given compound.
The term "acetylation", as used herein, refers to the attachment of -COMe group to
the N -amino group of a given compound. The term 'N -amino' refers to the amino group
located at the second position in the main carbon chain of a given compound.
The term "trityl", as used herein, refers to triphenylmethyl group and "Me" refers
to methyl group.
The term "alkoxy", as used herein, refers to -O-alkyl group, wherein the alkyl
group is selected from the group having Ci-C 6 carbon atom such as methyl, ethyl, propyl,
butyl, isobutyl, isopropyl, t-butyl, etc.
The term "Ph", as used herein, refers to phenyl group.
The term "about", as used herein, when used along with values assigned to certain
measurements and parameters means a variation of 10% from such values, or in case of a
range of values, means a 10% variation from both the lower and upper limits of such
ranges.
The phrase "lacosamide with improved impurity profile", as used herein, is
lacosamide wherein the Impurity-A is not detectable and Impurity-B is not more than
0.01% by HPLC. This also refers to the phrase "lacosamide substantially free of Impurity
A and Impurity B".
The phrase "lacosamide substantially free of Impurity A", as used herein, is meant
for lacosamide wherein the Impurity-A is not detectable by HPLC.
The phrase "lacosamide substantially free of Impurity B", as used herein, is meant
for lacosamide wherein the impurity-B is not more than 0.01% by HPLC.
The term "ambient temperature", as used herein, refers to temperature of the
surroundings wherein reaction is performed. Specifically "ambient temperature" is meant
as temperature or range that lies in between about 20°C to about 35°C.
Detailed Description of the Invention
The present invention is described in detail in the following aspects.
The first aspect of the present invention provides a process for preparation of
lacosamide comprising the following steps:
a) treating D-serine with a protecting reagent, wherein the number of moles of
protecting reagent is less than the number of moles of D-serine, to obtain a
compound of Formula V;
HN— R
FORMULA V
O-methylating the compound of Formula V obtained in step a) to produce
compound of Formula VI;
HN R
FORMULA VI
benzylaminating the compound of Formula VI to produce a compound of
Formula VII;
HN R
FORMULA VII
d) deprotecting the compound of Formula VII to produce a compound of
Formula IV; and
H2N
FORMULA IV
e) acetylating the compound of Formula IV in aqueous medium,
wherein R is the protecting group.
In an embodiment of this aspect of the invention, the lacosamide prepared is
substantially free of Impurity-A.
In another embodiment of this aspect of the invention, the lacosamide prepared is
substantially free of Impurity-B.
In another embodiment of this aspect of the invention, a process for the preparation
of lacosamide with improved impurity profile is provided.
In another embodiment of this aspect, about 0.8 to about 0.9 moles of protecting
reagent per mole of D-serine is used to prepare lacosamide.
The second aspect of the present invention provides a process for the preparation
amide comprising the following steps:
a) treating D-serine with protecting reagent, wherein the number of moles of the
protecting reagent is less then number of moles of D-serine, to obtain a
compound of Formula V;
HN—R
FORMULA V
benzylaminating the compound of Formula V to produce a compound of
Formula VIII;
HN R
FORMULA VIII
c) O-methylating the compound of Formula VIII to produce a compound of
Formula VII;
HN R
FORMULA VII
deprotecting compound of Formula VII to produce a compound of
IV; and
H2N
FORMULA IV
e) acetylating the compound of Formula IV in aqueous medium,
wherein R is protecting group.
In an embodiment of this aspect of the invention, the lacosamide prepared is
substantially free of Impurity-A.
In another embodiment of this aspect of the invention, the lacosamide prepared is
substantially free of Impurity-B.
In another embodiment of this aspect of the invention, lacosamide with improved
impurity profile is prepared.
In another embodiment of this aspect of the invention, about 0.8 moles to about 0.9
moles of the protecting reagent per mole of D-serine is used to prepare lacosamide.
The compound of Formula V of the present invention can be prepared by reacting
D-serine with a protecting reagent wherein the number of moles of the protecting reagent
is less than the number of moles of D-serine. Alternatively, the hydroxy and/or carboxylic
group of D-serine can be protected by a silyl protecting group like trimethylsilyl,
hexamethyldisilazane, etc., and then the free amino group in D-serine can be selectively
protected using a protecting reagent wherein the number of moles of the protecting reagent
is less than the number of moles of D-serine. Subsequently, the silyl protecting group can
be removed by hydrolysis providing a high yield of the compound of Formula V.
The O-methylation method of the compound of Formula V or Formula VIII, the
benzylamination method of compound of Formula V or Formula VI and the deprotection
method of compound of Formula VII are described in detail in the U.S. '472 publication,
the contents of which are incorporated herein by reference. These methods can also be
implemented in the processes of preparation of lacosamide of the present invention. These
methods can be applied for preparing lacosamide using any of the protecting reagents of
the present invention.
The deprotected compound of Formula IV is then acetylated to provide
lacosamide. Acetylation is performed in aqueous medium (i.e., comprises of water). For
this purpose, acetic anhydride, acetyl chloride, acetic acid, or the like may be used as the
acetylating agent. Acetylation can be performed optionally in the presence of a base. The
base can be a nitrogen containing base, i.e., pyridine, dimethylaminopyridine, etc.
Accordingly, the base is added to the aqueous solution of compound of Formula IV
and the acetylating agent is then slowly added to the mixture. The reaction is allowed to
proceed for about 2 hour at temperature ranging from 5°C to 40°C. The lacosamide
prepared is then isolated from the reaction mixture and purified.
The present inventors preferably use acetic anhydride as acetylating agent and
dimethylaminopyridine as base for the said acetylation purpose. Lacosamide so formed is
purified with the help of suitable organic solvents such as dichloromethane, toluene,
ethanol, ethyl acetate, etc.
A third aspect of the present invention provides a process for reducing the content
of "Impurity-A" in lacosamide during preparation of lacosamide that preparation
comprises a step of treating D-serine with the protecting reagent wherein number of moles
of the protecting reagent is less then number of moles of D-serine.
In an embodiment of this aspect of the present invention, the lacosamide prepared
is substantially free of Impurity-A.
In another embodiment of this aspect of the present invention, D-serine is treated
with the protecting reagent wherein about 0.8 moles to about 0.9 moles of the protecting
reagent per mole of D-serine is used.
D-serine is treated with the protecting reagent wherein number of moles of
protecting reagent is less than number of moles of D-serine. Alternatively, the hydroxy
and/or carboxylic group of the D-serine can be protected by silyl protecting group like
trimethylsilyl, hexamethyldisilazane, etc., and then the free amino group in the D-serine
can be selectively protected using protecting reagent wherein number of moles of
protecting reagent is less than number of moles of D-serine. Subsequently, the silyl
protecting group can be removed by hydrolysis reaction.
A fourth aspect of the present invention provides process for reducing content of
"Impurity-B" in lacosamide during the preparation of lacosamide, such preparation
comprises the step of acetylating the compound of Formula IV in aqueous medium.
NH2
FORMULA IV
In an embodiment of this aspect of the present invention, the lacosamide prepared
is substantially free of Impurity-B.
The compound of Formula IV is acetylated in aqueous medium (i.e., the medium
comprises water) to provide lacosamide having reduced content of "Impurity-B". For this
purpose, acetic anhydride, acetyl chloride, acetic acid, or the like may be used as the
acetylating agent. Acetylation can be optionally performed in the presence a base. The
base can be a nitrogen containing base, e.g., pyridine, dimethylaminopyridine etc.
Accordingly, base is added to the aqueous solution of compound of Formula IV
and acetylating agent is then slowly added to the mixture. The reaction is allowed to
proceed for about 2 hours at a temperature ranging from 5°C to 40°C. The lacosamide
prepared is then isolated from the reaction mixture and purified.
The present inventors preferably use acetic anhydride as the acetylating agent and
dimethylaminopyridine as the base for the said acetylation. Lacosamide so formed is
purified with the help of suitable organic solvents such as dichloromethane, toluene,
ethanol, ethyl acetate, etc.
A fifth aspect of the present invention provides lacosamide with an improved
impurity profile.
A sixth aspect of the present invention provides lacosamide substantially free of
Impurity-A.
A seventh aspect of the present invention provides lacosamide substantially free of
Impurity-B.
An eighth aspect of the present invention provides a composition comprising
pharmaceutically effective amount of lacosamide with improved impurity profile and
other pharmaceutically acceptable carrier, diluent and/or excipient.
While the present invention has been described in terms of its specific aspects,
certain modifications and equivalents will be apparent to those skilled in the art and are
intended to be included within the scope of the present invention.
In the following section aspects are described by way of example to illustrate the
processes of the invention. However, this is not intended in any way to limit the scope of
the present invention. Several variants of this example would be evident to persons
ordinarily skilled in the art.
Example 1: Preparation of Lacosamide
Step 1: Preparation of N-trityl-D-serine
HN C(C6H5 )3
All operations until reaction quenching were carried out under anhydrous
condition.
To the solution of D-Serine (100 g; 0.95 moles) in dichloromethane (1.0 L),
trimethylsilyl chloride (361.79 g) was added in 15 minutes to 30 minutes. It was refluxed
at 40°C for 20 minutes and then cooled to ambient temperature. To the resultant solution,
triethyl amine (433.27 g) dissolved in dichloromethane (200 ml) was added in 60 minutes
to 90 minutes. It was refluxed at 40°C to 45°C for 2 hours and then cooled to 0°C to -2°C.
To the cooled solution, methanol (45.72 g) diluted with dichloromethane (200 ml) was
added in 60 minutes to 75 minutes (exothermic reaction was observed). It was stirred for
15 minutes at 0°C to 5°C and then further raised to ambient temperature. At this
temperature, triethyl amine (96.28 g) was added in 15 minutes to 20 minutes and stirred
for 10 minutes. To the resultant reaction mixture, trityl chloride (238.78 g; 0.85 moles)
was added in 6 lots at an interval of 10 minutes at ambient temperature and stirred for 3
hours. The reaction mixture was cooled to 5°C and de-ionized water (500 ml) was added
to it at 5°C to 10°C. The reaction mixture was stirred for 15 minutes at the same
temperature and allowed to settle for 15 minutes. The organic layer (dichloromethane
layer) was separated and 5% citric acid monohydrate solution (500 ml) was added for 5
minutes to 10 minutes, stirred for 15 minutes at 5°C to 10°C and allowed to settle for 15
minutes. The organic layer (dichloromethane layer) was separated and de-ionized water
(500 ml) was added at 5°C to 10°C for 10 minutes to 15 minutes, stirred for 15 minutes
and allowed to settle. The organic layer obtained was separated and solvent was recovered
at atmospheric pressure up to 45°C. Traces of solvent (if any) were recovered under
vacuum at 35°C to 40°C. To the remaining residue, toluene (100 ml) was added and
heated to 50°C under stirring for 15 minutes. The solvent was recovered under vacuum at
45°C to 50 °C. Toluene (250 ml) was again added to the residue at 50°C and it was stirred
for 15 minutes. This was cooled to ambient temperature and then hexanes (500 ml) were
added. The mixture was stirred for 30 minutes and cooled further to 0°C to 5°C. It was
stirred at this temperature for 30 minutes. The solid obtained was filtered, washed with
hexanes (200 ml) and suck dried. It was further dried in a vacuum tray dryer at 50°C to
55°C until moisture content was NMT 0.5%.
Dry weight: 290 g.
Step 2: Preparation of lacosamide
Step 2A : In-situ Preparation of 0-methyl -N-trityl-D-serine
HN C(C6H5)3
All the operations until reaction quenching are carried out under anhydrous
condition.
To the stirred and cooled (at -15°C to -10°C) suspension of tetrahydrofuran (250
ml), imidazole (1.95 g) and sodium hydride (12.66 g), solid N-trityl-D- serine (50 g) was
added in lots at an interval of 10 minutes. It was stirred for 45 minutes at -15°C to -10°C
and then methyl iodide (40.85 g) was added to it at the same temperature (mild exothermic
reaction was observed). The temperature of the reaction mass was raised to -5°C to 0°C
and then it was stirred for 3 hours at the same temperature. De-ionized water (100 ml)
was added to it and the reaction mass was cooled to 0°C to 5°C. Acetic acid (9.8 ml) was
added to the reaction mass and pH of the mass was adjusted to 6.5 to 7.0. Tetrahydrofuran
was recovered completely under vacuum at 30°C to 35°C and dichloromethane (100 ml)
and de-ionized water (100 ml) were added to the reaction mass at ambient temperature.
This was stirred and allowed to settle for 15 minutes. The layers were separated.
Dichloromethane (50 ml x 2) was added to the aqueous layer, stirred, allowed to settle and
layers were separated. The obtained organic layers were combined and de-ionized water
(100 ml) was added to it. This was stirred, allowed to settle and then organic layer was
separated. Anhydrous sodium sulphate (5 g) was added to the organic layer. It was stirred
for 20 minutes and filtered through hyflo bed.
Step 2B: In-situ Preparation of N-benzyl-0-methyl -N2-trityl-D-serinamide
HN C(C6H5)3
All the operations until reaction quenching are carried out under anhydrous
condition.
Preparation of Solution A
The filtered organic layer (dichloromethane layer) obtained as above was cooled to
15°C to 20°C and N-methyl morpholine (NMM; 15.62 g) was added to it at 15°C to 20°C
in 10 minutes to15 minutes. The temperature of the solution was raised to 20°C to 25°C
and it was stirred for 15 minutes at the same temperature.
Preparation of Solution B
Ethyl chloroformate (16.46 ml) was added to dichloromethane (104 ml) at ambient
temperature. It was cooled to -10°C to -15°C and stirred for 15 minutes.
The Solution A was added to the Solution B in 60 minutes to 90 minutes at -15°C
to -10°C and stirred for 30 minutes at the same temperature.
Preparation of solution of benzyl amine
A solution of benzyl amine (18.38 g) in dichloromethane (52 ml) at ambient
temperature was added to the above prepared solution in 60 minutes to 90 minutes at
-15°C to -10°C and stirred for 60 minutes at -15°C to 0°C. The obtained reaction mass
was cooled to -10°C to -15°C and then N-methyl morpholine (NMM; 5.20 g) was added in
10 minutes to 15 minutes. It was stirred for 15 minutes at the same temperature, and then
ethyl chloroformate (5.48 g) was added and again stirred for 15 minutes. A solution of
benzyl amine (6.12 g) in dichloromethane (26 ml) was slowly added at -15°C to -10°C.
The reaction mass, so obtained, was heated to 15°C to 20°C and stirred for 60 minutes at
20°C to 25°C. De-ionized water (104 ml) was added to it, stirred and allowed to settle for
15 minutes. The organic layer was separated and a precooled solution of citric acid (6 g in
of 104 ml water) was added at 20°C to 25°C. It was stirred, allowed to settle for 15
minutes and then organic layer was separated. To the organic layer, de-ionized water (104
ml) was added at 25°C to 20°C, stirred, allowed to settle for 15 minutes and then organic
layer was separated.
Step 2C: In-situ preparation of N-benzyl-0-methyl-D-serinamide
H2N
To the organic layer (the dichloromethane layer) as obtained above, concentrated
hydrochloric acid (23.22 g dissolved in 65 ml de-ionized water) was added in 15 minutes
to 20 minutes at 25°C to 30°C. It was stirred for 60 minutes at the same temperature and
allowed to settle for 15 minutes. The layers were separated. The organic and aqueous
layers were separately collected. To the organic layer, de-ionized water (65 ml) was
added, stirred and allowed to settle for 15 minutes. Again, the layers were separated and
the organic and aqueous layers were separately collected. Both aqueous layers obtained
were combined and dichloromethane (32.5 ml) was added at 25°C to 30°C, stirred and
allowed to settle for 15 minutes. The layers were separated and collected separately.
From the aqueous layer, traces of dichloromethane were recovered under vacuum at 30°C
to 35°C. The aqueous layer was cooled to 25°C to 30°C and then filtered through a 0.45
micron filter. To the filtered layer, hexanes (65 ml) were added at ambient temperature,
stirred and allowed to settle for 15 minutes. These layers were separated. The aqueous
layer was again separated and cooled to 20°C to 25°C. The pH of the aqueous layer was
adjusted to 11 to 11.5 by using 10% sodium hydroxide solution (88 ml) at 20°C to 25°C.
Step 2D: Preparation of lacosamide
To the aqueous solution obtained as above, dimethyl amino pyridine (DMAP; 0.34
g) was added at ambient temperature. Acetic anhydride (14.68 g) was slowly added to the
solution at 25°C to 30°C. It was stirred for 2 hours at the same temperature and then 10%
sodium hydroxide (120 ml) was added into it to adjust pH 6.5-7.5 at 25-30°C.
Dichloromethane (325 ml) was added to it and the reaction mass was allowed to settle for
15 minutes. The layers were separately collected. To the aqueous layer, dichloromethane
(260 ml) was added, stirred and allowed to settle for 15 minutes. The layers were
separated. The organic layers were combined and washed with de-ionized water (65 ml).
It was stirred for 10 minutes and allowed to settle for 15 minutes. The layers were
separately collected. To the organic layer, activated carbon (1.5 g) was added at ambient
temperature, stirred and then filtered through a hyflo bed at 25°C to 30°C. The hyflo bed
was washed with dichloromethane (30 ml) at 25°C to 30°C. Solvent was recovered from
the filtered and washed organic layer at atmospheric pressure and at 35°C to 40°C. Traces
of solvent, if any, were recovered under vacuum at 35°C to 40°C to get a solid. Ethyl
acetate (30 ml) was added to the solid at 25°C to 30°C. It was heated to 45°C to 50°C and
stirred for 15 minutes. Solvent was recovered at atmospheric pressure and at 45°C to
50°C. Ethyl acetate (210 ml) was again added at ambient temperature. It was stirred for
60 minutes at ambient temperature. The product obtained was filtered and suck dried for
60 minutes. Ethyl acetate (540 ml) was added to the wet product obtained and heated to
reflux to obtain clear solution. The solution was stirred for 5 minutes at reflux
temperature and then cooled to 30°C in 1 hour. It was again stirred for 30 minutes at 25°C
to 30°C and then cooled to 0°C to 5°C. Toluene (90 ml) was added to it at the same
temperature and stirred for 30 minutes. The solid obtained was filtered and washed with
toluene (60 ml) at ambient temperature. The wet solid obtained was dried under vacuum
at 60°C to 65°C.
Dried weight: 17.5 g (loss on drying was 0.1%)
Impurity content of Formula II: Not detectable by HPLC
Impurity content of Formula III: 0.01% by HPLC
Impurity content of Formula II and/or Formula III in lacosamide were measured by
HPLC (Agilent 1100 series) having photodiode array detectors. The analysis was
performed at wavelength of 210 nm.
We claim:
1. A process for the preparation of lacosamide comprising the steps of:
a) treating D-serine with a protecting reagent, wherein number of moles of the
protecting reagent is less than the number of moles of D-serine, to obtain a
compound of Formula V;
HN— R
FORMULA V
b) O-methylating the compound of Formula V obtained in step a) to produce a
compound of Formula VI;
O
MeO' OH
HN R
FORMULA VI
c) benzylaminating the compound of Formula VI to produce a compound of
Formula VII;
HN R
FORMULA VII
d) deprotecting the compound of Formula VII to produce a compound of
Formula IV; and
FORMULA IV
e) acetylating the compound of Formula IV in aqueous medium
wherein R is protecting group.
2. The process of claim 1 wherein the lacosamide prepared is substantially free of
Impurity-A and/or Impurity-B.
3. A process for the preparation of lacosamide comprising the steps of:
a) treating D-serine with a protecting reagent, wherein number of moles of the
protecting reagent is less than the number of moles of D-serine, to obtain a
compound of Formula V;
HN— R
FORMULA V
b) benzylaminating the compound of Formula V to produce a compound of
Formula VIII;
HN R
FORMULA VIII
c) O-methylating the compound of Formula VIII to produce a compound of
Formula VII;
HN R
FORMULA VII
d) deprotecting compound of Formula VII to produce a compound of Formula
IV; and
H2N
FORMULA IV
e) acetylating the compound of Formula IV in aqueous medium
wherein R is protecting group.
4. The process of claim 3 wherein the lacosamide prepared is substantially free of
Impurity-A and/or Impurity-B.
5. A process for reducing the content of "Impurity-A" in lacosamide during the
preparation of lacosamide that comprises the step of treating D-serine with a protecting
reagent wherein the number of moles of the protecting reagent is less than the number of
moles of D-serine.
6. The process of claim 5 wherein the lacosamide prepared is substantially free of
Impurity-A.
7. A process for reducing the content of "Impurity-B" in lacosamide during the
preparation of lacosamide that comprises the step of acetylating the compound of Formula
IV in aqueous medium.
O
MeQ \ NHCHgPh
NH2
FORMULA IV
8. The process of claim 7 wherein lacosamide prepared is substantially free of
Impurity-B.
9. Lacosamide substantially free of Impurity-A and Impurity-B.
10. Lacosamide substantially free of Impurity-A.
11. Lacosamide substantially free of Impurity-B.