PRODUCT COMPRISING STEVIA

TECHNICAL FIELD OF THE INVENTION
The present invention relates to products comprising sweetening agents derived from
Stevia rebaudiana. In particular the invention relates to the use of solids from Aspalathus
linearis for providing improved products comprising sweetening agents derived from Stevia
rebaudiana.

BACKGROUND OF THE INVENTION
Stevia rebaudiana is a small perennial shrub that is native to South America. Both the
plant Stevia rebaudiana and the sweet compounds derived from it are often referred to
simply as "stevia". Stevia is used in several parts of the world as a non-caloric sweetener.
For example, the leaves of S. rebaudiana have been used for centuries by the natives of
Paraguay to sweeten beverages, and stevia sweeteners have been used in Japan since
the 1970s.
Several diterpene glycosides have been extracted and purified from Stevia rebaudiana,
including a number of intensely sweet steviol glycosides. These glycosides are found in
the stems, seeds and leaves of the S. rebaudiana plant, and are present at the highest
concentration in the leaves (where they may comprise from 10 to 20% of the leaves by dry
weight). The predominant steviol glycosides found in the leaves of S. rebaudiana are
stevioside (typically 9.1 % by dry weight) and rebaudioside A (around 3.8% by dry weight).
Although stevia in its raw form is incredibly sweet, it also has a subtle liquorice essence
and bitter notes are perceived at the back end of its taste profile. Both stevioside and
rebaudioside A have been reported to contribute to the bitter aftertaste, particularly when
used at concentrations corresponding to high sucrose equivalency (SE) levels.
Stevia has enormous commercial potential as a natural non-caloric sweetener. However,
it is necessary to achieve an improved taste profile in order for this potential to be realised.
Several approaches have been reported. For example, international patent application
WO 2008/1 12979 (The Concentrate Manufacturing Company of Ireland) discloses the use
of tea extracts for reducing the off-taste of non-nutritive sweeteners such as stevia.
However, the beverages disclosed therein do not comprise solids from Aspalathus linearis.
We have found that the use of solids from Aspalathus linearis (rooibos) unexpectedly
allows for the formulation of products with excellent taste characteristics when natural
sweetening agents derived from Stevia rebaudiana (Bertoni) are incorporated therein.

TESTS AND DEFINITIONS
As used herein the term "comprising" encompasses the terms "consisting essentially of"
and "consisting of". All percentages and ratios contained herein are calculated by weight
unless otherwise indicated. It should be noted that in specifying any range of values or
amount, any particular upper value or amount can be associated with any particular lower
value or amount.
The disclosure of the invention as found herein is to be considered to cover all
embodiments as found in the claims as being multiply dependent upon each other
irrespective of the fact that claims may be found with multiple dependency or redundancy.

Stevia
The term "stevia-derived" is used herein to refer to material that can be obtained from the
stems, leaves and or seeds of the Stevia rebaudiana (Bertoni) plant.
A number of intensely sweet steviol glycosides are known to occur naturally in the Stevia
rebaudiana plant. The steviol glycosides include stevioside, steviobioside, rebaudioside A,
rebaudioside B, rebaudioside C, rebaudioside D, rebaudioside E, dulcoside A and
dulcoside B. As used herein the terms "sweetening agent derived from Stevia rebaudiana"
and "stevia-derived sweetening agent" are used interchangeably and refer to a sweetening
agent comprising one or more steviol glycoside.
The term "stevia-sweetened" is used herein to refer to a product which comprises a steviaderived
sweetening agent. Stevia-sweetened products may additionally comprise one or
more nutritive and or non-nutritive sweetener that is not derived from S. rebaudiana. For
example in certain embodiments stevia-sweetened products may additionally comprise
one or more sweetener selected from sucrose, glucose, fructose, lactose, trehalose,
maltose, aspartame, saccharin, acesulfame K, erythritol, xylitol, sorbitol, mannitol, glycerol,
maltitol, lactitol, isomalt, alitame, thaumatin, cyclamate, glycyrrhizin, neohesperidine
dihydrochalcone, sucralose, monellin, neotame, mogroside V, and mixtures thereof.
Rooibos
Aspalathus linearis (also known as rooibos or redbush) is a shrub-like leguminous bush
native to the Western Cape region of South Africa. As used herein the terms "solids from
Aspalathus linearis" and "rooibos solids" are used interchangeably and refer to dry material
that can be extracted with a solvent from the leaves and or stems of the plant Aspalathus
linearis. The term "rooibos extract" refers to an extract that comprises solids from
Aspalathus linearis.
Beverage
As used herein the term "beverage" refers to a substantially aqueous drinkable
composition suitable for human consumption. Preferably the beverage comprises at least
85% water by weight of the beverage, more preferably at least 90% and most preferably
from 95 to 99.9%.
As used herein the term "packaged" means that the beverage is contained within a sealed
package. Non-limiting examples of suitable packages include bottles, cans, cartons,
pouches and sachets.
Tea
As used herein "tea" refers to material from Camellia sinensis var. sinensis and/or Camellia
sinensis var. assamica. "Black tea" refers to substantially fermented tea, "oolong tea"
refers to partially fermented tea and "green tea" refers to substantially unfermented tea.
"Fermentation" refers to the oxidative and hydrolytic process that tea undergoes when
certain endogenous enzymes and substrates are brought together. Fermentation may be
supplemented by the action of exogenous enzymes such as oxidases, laccases and
peroxidises.
Tea-based beverage
As used herein the term "tea-based beverage" refers to a beverage comprising at least
0.01% tea solids by weight of the beverage. Preferably the tea-based beverage
comprises from 0.04 to 3% tea solids, more preferably from 0.06 to 2%, most preferably
from 0.1 to 1%. The term "tea solids" refers to dry material extractable from the leaves of
the plant Camellia sinensis var. sinensis and/or Camellia sinensis var. assamica.
SUMMARY OF THE INVENTION
In a first aspect, the present invention provides a product comprising a sweetening agent
derived from Stevia rebaudiana that comprises rebaudioside A, characterised in that the
product comprises solids from Aspalathus linearis. We have found that the use of solids
from Aspalathus linearis allows the formulation of stevia-sweetened products with excellent
taste characteristics. In particular, we have found that use of solids from Aspalathus
linearis can be used to alleviate and/or eliminate the bitterness aftertaste and/or off-notes
of stevia-derived sweetening agents that comprise rebaudioside A.
In another aspect, the present invention provides use of solids from Aspalathus linearis for
reducing or eliminating bitterness aftertaste of a sweetening agent derived from Stevia
rebaudiana. In a preferred embodiment, solids from Aspalathus linearis are used to
improve the taste profile of sweetening agents that comprise rebaudioside A.
In a further aspect, the present invention provides a method of manufacturing a product
comprising rebaudioside A and solids from Aspalathus linearis, wherein a substance
comprising the rebaudioside A is combined with a substance comprising the solids from
Aspalathus linearis. It is preferred that the product is a beverage product.
DETAILED DESCRIPTION
The product of the present invention may be a food or a beverage product, or a precursor
for preparing such a product. Preferably the product is a beverage, more preferably it is a
packaged beverage.
The mass of the product will depend on the format of the product. For convenience of
transport and handling it is preferred that the product has a total mass of 0.5 g to 2100 g,
more preferably 1 g to 1100 g and most preferably 2 g to 550 g.
The development of products employing non-nutritive sweeteners presents challenges in
addressing associated bitterness and/or off-tastes. The stevia-derived sweetening agent
of the present invention comprises rebaudioside A, which is the sweetest and most stable
of the steviol glycosides. Rebaudioside A has been reported to have less bitterness
aftertaste and/or off-notes than other sweet compounds derived from the S. rebaudiana
plant. Therefore it is desirable that at least 15% by weight of the stevia-derived sweetening
agent comprises rebaudioside A. In a preferred embodiment at least 50% by weight of the
stevia-derived sweetening agent comprises rebaudioside A, more preferably at least 75%,
still more preferably at least 95% and most preferably from 97% to 100%.
In order produce a product with an initial sweetness that lingers in a pleasing manner it is
preferred that the product comprises at least 0.001% by weight of the stevia-derived
sweetening agent. More preferably the product comprises from 0.005 to 5.0% by weight
of the sweetening agent, still more preferably from 0.01 to 3.5% and most preferably from
0.01 5 to 2.5%.
We have found that solids from Aspalathus linearis bring a desirable complexity and/or
smoothness to the taste profile of stevia-sweetened products. In order to achieve a
product with a desirable taste profile it is preferred that the product comprises at least
0.001 % solids from Aspalathus linearis by weight, more preferably from 0.005 to 5.0%, still
more preferably from 0.01 to 3.5% and most preferably from 0.01 5 to 2.5%.
e have found that the use of solids from Aspalathus linearis is well-suited to delivering
stevia-sweetened beverages with desirable taste characteristics. Such beverages are
found to lack the bitterness aftertaste and or off-notes typically associated with steviaderived
sweeteners. Furthermore, it appears that the use of rooibos solids in steviasweetened
beverage products results in a smooth taste profile and/or adds a pleasing
complexity to the flavour of the beverage. Therefore, in a preferred embodiment the
product is a beverage, preferably a packaged beverage. It is also envisaged that the
product of the invention may be a beverage precursor.
It is often desirable to sweeten tea-based beverages, especially if they contain high levels
of bitter-tasting polyphenols (e.g. catechins, theaflavins). Therefore, in one particular
embodiment, it is preferred that the product of the present invention is a tea-based
beverage.
Where the product is a beverage product, it is preferred that it comprises solids from
Aspalathus linearis in an amount from 10 to 1000 mg/L, more preferably from 70 to 500
mg/L, still more preferably from 100 to 450 mg/L and most preferably from 150 to 400
mg/L.
Where the product is a beverage product, it is preferred that it comprises from 10 to 600
mg/L of the stevia-derived sweetening agent, more preferably from 100 to 500 mg/L and
most preferably from 150 to 400 mg/L.
From a standpoint of microbial stability and taste, it is preferred that the product has an
acidic pH. In particular, the pH (at 20°C) may be from 2 to 7, more preferably from 2 to 5,
most preferably from 2.5 to 4.
The product of the present invention may be manufactured by any suitable means. The
product is preferably manufactured by a method wherein a substance comprising a
sweetening agent derived from Stevia rebaudiana that comprises rebaudioside A is
combined with a substance comprising solids from Aspalathus linearis. Preferably at least
15% by weight of the sweetening agent derived from Stevia rebaudiana comprises
rebaudioside A, more preferably at least 50% by weight, still more preferably at least 75%
by weight, more preferably still at least 95% by weight and most preferably from 97% to
100% by weight.
EXAMPLES
The present invention will now be illustrated by reference to the following non-limiting
examples.
Example 1
Solids from Aspalathus linearis ("rooibos solids") were assessed for their effect on the taste
profile of stevia-sweetened beverage products. Stevia-sweetened beverage products
comprising different types of tea solids from Camellia sinensis (green tea, black tea) were
also assessed. The products contained 175 mg/L of either rooibos solids or tea solids.
The products were qualitatively assessed in a tasting session. The products were tasted
blind (i.e. the individuals did not know the identity of the products presented). The steviasweetened
product comprising rooibos solids was perceived as having a more favourable
taste profile than the equivalent products comprising either black tea solids or green tea
solids.
Example 2
Three mid-calorie beverage products were prepared according to an established
formulation comprising flavourings and preservatives. Each beverage product was
sweetened in a uniform manner using a combination of sucrose and rebaudioside A.
Rooibos solids (provided by rooibos extract I) were present in products 2 and 3, but not in
product 1 (see Table 1).
TABLE 1
* From PureCircle (97% rebaudioside A)
†From Plantextrakt GmbH & Co. KG (35% rooibos solids).
The beverage products were filled into 330 ml cans and sealed. The cans were then
pasteurised at 70°C for 10 minutes in a batch pasteuriser.
The beverage products were qualitatively assessed in a tasting session. The individuals
who participated in this session did not know the composition of the products presented.
Product 1 was perceived to have bitter notes at the back end of the taste profile. However,
the bitter aftertaste was noticeable reduced in product 2 (175 mg/L rooibos solids) and
product 3 (350 mg/L rooibos solids) compared to product 1. Furthermore, incorporating
rooibos solids was judged to bring a desirable complexity and smoothness to the taste
profile of these products. An initial sweetness that lingered on in a pleasing manner was
also perceived on drinking products 2 and 3.
Example 3
Three diet beverage products were prepared according to an established formulation
comprising flavourings and preservatives. Each beverage product was sweetened in a
uniform manner using a combination of erythritol and rebaudioside A. Rooibos solids
(provided by rooibos extract I) were present in products 5 and 6, but not in the product 4
(see Table 2).
TABLE 2
* From PureCircle (97% rebaudioside A)
†From Plantextrakt GmbH & Co. KG (35% rooibos solids).
The beverage products were filled into 330 ml cans and sealed. The cans were then
pasteurised at 70°C for 10 minutes in a batch pasteuriser.
The beverage products were qualitatively assessed in a tasting session. The individuals
who participated in this session did not know the composition of the products presented.
Product 4 was found to have an undesirable taste profile. The taste panel reported that
this product lacked sweetness, and that the flavour was bitter and quite sour. Both product
5 (175 mg/L rooibos solids) and product 6 (350 mg/L rooibos solids) were assessed as
having a more desirable taste profile than product 4. The addition of rooibos solids was
judged to add complexity to the flavour and to provide smoothness to the taste profile.
Example 4
Five mid-calorie beverage products were prepared according to an established formulation
comprising flavourings and preservatives. Each beverage product was sweetened in a
uniform manner using a combination of sucrose and rebaudioside A. Product A was a
control product that did not contain rooibos solids. Products B, C, D and E (collectively
referred to as "rooibos products") all contained 175 mg/L rooibos solids. As can be seen
from Table 3, the rooibos solids in products B, C, D and E were provided by rooibos
extracts from different sources.
TABLE 3
* From PureCircle (97% rebaudioside A)
+From Plantextrakt GmbH & Co. KG (35% rooibos solids).
*From Plantextrakt GmbH & Co. KG (36% rooibos solids).
†From Afriplex (Ply) Ltd. ( 100% rooibos solids).
* * From Afriplex (Ply) Ltd. (30% rooibos solids).
The beverage products were filled into 330 ml cans and sealed. The cans were then
pasteurised at 70°C for 10 minutes in a batch pasteuriser.
The beverage products were qualitatively assessed in a tasting session. The individuals
who participated in this session did not know the composition of the products presented.
Product A was perceived to have bitter notes at the back end of the taste profile. The taste
panel judged each of products B, C, D and E to have an improved aftertaste and a
smoother taste profile than product A. No marked difference between the four rooibos
products were reported, although product D (formulated with a rooibos extract comprising
100% rooibos solids) was felt to have a slightly cleaner flavour.
The data from the tasting session clearly indicated that the four rooibos products are not
significantly different to each other in taste, flavour or mouthfeel. This implies that the
presence of rooibos solids (regardless of their source) is important when it comes to
formulating stevia-sweetened products with excellent taste characteristics.
A product comprising a sweetening agent derived from Stevia rebaudiana that
comprises rebaudioside A, characterised in that the product comprises solids from
Aspalathus linearis.
A product as claimed in claim 1 wherein at least 50% by weight of the sweetening
agent comprises rebaudioside A.
A product as claimed in claim 1 or claim 2 wherein the product comprises at least
0.001 % by weight of the sweetening agent.
A product as claimed in any one of the preceding claims wherein the product
comprises from 0.005% to 5.0% by weight of the sweetening agent.
A product as claimed in any one of the preceding claims wherein the product
comprises at least 0.001 % by weight solids from Aspalathus linearis.
A product as claimed in any one of the preceding claims wherein the product
comprises solids from Aspalathus linearis in an amount from 0.005% to 5.0% by
weight.
A product as claimed in any one of the preceding claims wherein the product is a
beverage, preferably a tea-based beverage.
A product as claimed in claim 7 wherein the product comprises solids from
Aspalathus linearis in an amount from 10 to 1000 mg/L.
A product as claimed in claim 7 or claim 8 wherein the product comprises the
sweetening agent in an amount from 10 to 600 mg/L.
10. A product as claimed in any one of claims 7 to 9 wherein the product has a pH of
from 2 to 7.
11. A product as claimed in any one of the preceding claims wherein the product
additionally comprises one or more nutritive and/or non-nutritive sweetener that is
not derived from Stevia rebaudiana.
12. Use of solids from Aspalathus linearis for reducing or eliminating bitterness aftertaste
of a sweetening agent derived from Stevia rebaudiana.
13. A use according to claim 11 wherein the sweetening agent comprises rebaudioside
A.
14. A method of manufacturing a product comprising rebaudioside A and solids from
Aspalathus linearis, wherein a substance comprising the rebaudioside A is combined
with a substance comprising the solids from Aspalathus linearis.
15. A method according to claim 13 wherein the product is a beverage.