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Production Method For Biofuel

Abstract: The present invention provides a production method for biofuel based on a technology to convert carbon-dioxide as a carbon source through photosynthesis by photosynthetic microorganisms to biomass and produce biofuel of the biomass. The production method for biofuel of the present invention comprises a culturing process (S1) of culturing in a culture solution photosynthetic microorganisms which store oils  fats and carbohydrates in cells of the photosynthetic microorganisms  an oil and fat conversion process (S2) of converting the carbohydrates stored in the cells of the photosynthetic microorganisms cultured in the culture apparatus to oils and fats  an extraction process (S3) of extracting the oils and fats out of the cells of the photosynthetic microorganisms  and a reforming process (S4) to reform the extracted oils and fats.

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Patent Information

Application #
Filing Date
23 November 2012
Publication Number
12/2016
Publication Type
INA
Invention Field
BIOTECHNOLOGY
Status
Email
Parent Application
Patent Number
Legal Status
Grant Date
2019-02-19
Renewal Date

Applicants

JX Nippon Oil & Energy Corporation
6-3  Otemachi 2-chome  Chiyoda-ku  Tokyo 100-8162  Japan
euglena Co.  Ltd.
University of Tokyo  Entrepreneur Plaza 7F  University of Tokyo Hongo Campus  7-3-1  Hongo  Bunkyo-ku  Tokyo 113-0033  Japan

Inventors

1. KATO Hiroaki
c/o Hitachi Plant Technologies  Ltd.  5-2  Higashi-Ikebukuro 4-chome  Toshima-ku  Tokyo 170-8466  Japan
2. YAMASHITA Ko
c/o Hitachi Plant Technologies  Ltd.  5-2  Higashi-Ikebukuro 4-chome  Toshima-ku  Tokyo 170-8466  Japan
3. FUKUSHIMA Yukio
c/o Hitachi Plant Technologies  Ltd.  5-2  Higashi-Ikebukuro 4-chome  Toshima-ku  Tokyo 170-8466  Japan
4. AMANO Ken
c/o Hitachi Plant Technologies  Ltd.  5-2  Higashi-Ikebukuro 4-chome  Toshima-ku  Tokyo 170-8466  Japan
5. KANEKO Takashi
c/o JX Nippon Oil & Energy Corporation  6-3  Otemachi 2-chome  Chiyoda-ku  Tokyo 100-8162  Japan
6. UEDA Iwao
c/o JX Nippon Oil & Energy Corporation  6-3  Otemachi 2-chome  Chiyoda-ku  Tokyo 100-8162  Japan
7. AOKI Nobuo
c/o JX Nippon Oil & Energy Corporation  6-3  Otemachi 2-chome  Chiyoda-ku  Tokyo 100-8162  Japan
8. SUZUKI Kengo
c/o euglena Co.  Ltd.  University of Tokyo  Entrepreneur Plaza 7F  University of Tokyo Hongo Campus  7-3-1  Hongo  Bunkyo-ku  Tokyo 113-0033  Japan
9. ARASHIDA Ryo
c/o euglena Co.  Ltd.  University of Tokyo  Entrepreneur Plaza 7F  University of Tokyo Hongo Campus  7-3-1  Hongo  Bunkyo-ku  Tokyo 113-0033  Japan
10. NAKANO Ryohei
c/o euglena Co.  Ltd.  University of Tokyo  Entrepreneur Plaza 7F  University of Tokyo Hongo Campus  7-3-1  Hongo  Bunkyo-ku  Tokyo 113-0033  Japan

Specification

Production Method For Biofuel . BACKGROUND OF THE INVENTION FIELD OF THE INVENTION l000ll The present invention relates to a production method for a biofuel to produce the biofuel. Coo021 " The biofuel is a fuel produced from a raw material derived from biomass. If the biomass has a photosynthesis capability as ordinary plants have, it is possible to produce a fuel that has a smaller effect on the environment, because oils, fats and carbohydrates produced through the biomass from light energy, carbon-dioxide arc? raw materials for the biofuel. There are biofuels such as a bioethanol produced through alcohol fermentation of saccharified carbohydrate and a biodiesel oil and a biojetfuel which are produced from neutral lipids such as ,waxesters anti triglycerides COO031 Although soy beans, corns and palms have been well known to be used for raw materials for the biofuel, there is an issuethat using edible crops such as these crops leads to shortage of foods. Alternatively, non-edible crops such as jatropha and cknelina are produced for the biofuel and there is a problem with these crops having a low yield for a unit area. 100041 On tho other haid, it is lrnown that photosynthetic microorga~lismsa nd. prot;ozoa Iivirig in many lakes and marshes have the same photosynthesis capability as plants have and synthesize oils, fats and carbohydrates from water and carbon-dioxide and store in their cells an amount of oils, fats and carbohydrates, which corresponds to several tens percents of a dry weight of the photosynthetic microorganisms and protozoa. The photosynth.et,ic microorganisms and protozoa are capable of producirlg a larger amount of oils, fats and carbohydrates than plants do and produce a more than t.e. n times larger ' 1 . . amount of oils, fats and carbohydrates for unit area than palm,which is said to be capable of producing a large amount of palm oils. [00051 A patent document 1 describes fixing carbon-dioxide by culturing algae which are capable of photosynthesis by radiating the artificial light whose wave lengLh and Light intensity are artificially adjusted. The patent document 1 further describes a means, a method and a cultivation device to produce a target substance by fixing carbon-dioxide. Moreover the patent document lgroposes Gxing carbon-dioxide with photosynthetic microorganisms and makinguse of the fixed carbon-dioxide as a basic fuel fa; the biofuel as an example. PRIOR ART DOCUMENT PATENT DOCUMENT [0006] Patent Document 1 : JP2010-057485A OBJECTIVE TOBE ACIlIEVED BY THE INVENTION [00071 . . However the pat,ent document; 1 does not disclose anything about how a biofuel is produced from the fixed carbon-dioxide. When the carbon-dioxide gas is reduced by making use of photosynthesis capability of microorganisms, the weight of ihe biomass increases in proportion to -the amount of the fixed. carbondioxide and it is difficult to make an industrial use of this invention if the biomass with the increased weight is effectively used. lb008l The present invenkion is intended to provide a biofuel production method i~iilusiveo f a series of technologies to convert carbon-dioxide which is a carbonsource to the biomass through photosyni;hcsis by photosynthetic microorganisms and subsequently produce a biofuel. . > MEANS TO ACHIEVE THE OBJECTIVE Eo0091 In order to achieve the objective above mentioned, the production method for biofuel of the present invention comprises a culturing process of culturing in a culture soiution photosynthetic microorganisms which store oils, fats and carbohydrates in cells of the photosynthetic microorganisms, an oil and fat conversion process of converting the carbohydrates stored in the cells of the photosynthetic microorganisms cultured in the culture apparatus to oils and fats, an extraction process of extracting the oils and fats.out of the cells of the photosynthetic microorganisms, and a reforming process to reform the extracted oils and fats. [oolol According to the production method for biofuel of the p~esenitn vention, the photosynthetic microorganisms are cultured in. the culturing process, carbon. dioxide wkrch is a carbon source is converted through photosynthesis by the photosynthetic microorganisms cnltured in the culture solution to oils, fats and carbohydrates which are stored in cells of the photosynthetic microorganisms and the stored carbohydrates are converted to oils and fats. As a result, a larger amount of oils and fats than produced i.n a. ny of the current produdion methods is efficiently produced. lo addition, according to the production method for biofuel of the present invention, oils and fats are subsequently extracted in an extraction process and the extracted oils, fats are reformed in a reforming process that follows the extraction process and biofuel is produced. EFFECT OF THE INVENTION I O O l l l The present invention is intended to provide a biofuel production method inclusive of a series of technologies to convert carbon-dioxide whicb. is a carbon source to the biomass th~oughp hotosynthesis by photosynthetic microorganisms ", and subsequently produce a biofuel. Moreover the present invention e&ables producing a larger amount of a ' biofuel than any of the current biofuel production methods, because it has a production process inclusive of an oil and fat conversion process. BRIEF DESCRIPTION OF DRAWINGS [oo121 Fig.1 is a flowchart explaining an embodiment for a biofuel production method of the present invention. Fig.2 is a block diagram explaining an embodiment for the biofuel production apparatus. DETAILED DESCRIPTION OF THE EMBODIMENTS CO013l Hereinafter an embodiment of a biofuel production method of the present invention is explained with reference to Fig.1. [00141 As shown in Pig.1, the biofuel production mc:thod of the present invention inchides a culturing process Sl, an oil and fa.t conversion process S2, an extraction process S3 and a reforming process S4. loo l rjl The culturing process S1 to be performed it first; is a process to culture photosynthetic microorganisms which store oils, fats and carbohydrates in their cells in a culture solution. Euglena is considered to be photosynthetic microorganisms to be used lor the present invention. Euglena includes Euglena gracilis which belongs to a group of flagellates and is well known as a motile alga. Most of the Euglena have chlorophylls and live autotrophically through photosynthesis and there are some of theEuglena which are of a predator type or absorb nourishment from other organisms. The Euglena is classified in both the zoology and the botany. In the zoology, a Euglenida, which is one of orders belonging to a Mastigophora and a Phytomastigophora in Protozoa, consists of three &borders, Eulenoidina, Peranemoidina and Petalomonadoidina. The Eulenoidina includes Genera, Euglena, Trachelemonas, Strombonas, ~ h a c u sL, epocinelis and Colacium. In the botany, a Euglenales, which belongs to a Euglenophyceae in a Euglenophyta, includes genera such as the Euglena, as is the same way as in the zoology. Resides the Euglena used for the present invention may be one or more of Cyanobacteria, Green algae and Trebouxiophyceae, Bangiophyceae, Prasinophyceae, Bacillasiophyceae, Coccolithophorid, Dinophyceae, Eustigmatophyceae and Chrysophyceae. [00161 Examples of the Cyanobacteria for the present invention are Chroococcacae, Stigonematacae, Mastigocladacae and Oscillatroriacae and other examples are Synechococcus such as Synechococcus lividus and Synechococcus elongatus, Synechocystis such as Synechocystis minervae, Mastigocladus such as Mastigocladus laminosus, Phormidium such as Phormidium laminosus, Symploca such as Symploca thermalis, Aphanocapsa such as .A.phanocapsa thermalis and Fisherella. Furthermore Anabanena variabilis ATCC 29413 which belongs. to genus Anabaena, Cyanothece sp. ATCC 51.142 which belongs to genus Cyanothece, Synechoeoccus sp. PCC 794.2 which belongs to genus ~~nechococ&a~nsd; Anacystis nidulans and Thermosynechococc~e~lso ngatus which belong to Axlacystis may be used for the present invention. 100171 'Examples of green algae and Trebouxiophyceae .for the present invention are Cephaleuros such as Chlorella inclusive of Parachlorella separated from Chlorella according to phylogenesis, Chlamydomonas, Dunaliella, Scenedesmus, Botlyococcus, Stichococcus, Nannochloris and Desmodesmus. To be specific, examples of green algae and Trebouxiophyceae for the present invention are Chlorella vulgaris and ChloreUa saccharop'hila included in Chlorella, Dunaliella such as Dunaliella salina. Dunaliella tertiolecta and Parachlorella kessleri (Chlorella kessleri) whose basic properties Like photosynthesis are the same as Chlorella and Dunaliella and which are - classiGed in Trebouxiophyceae according to the molecular phylogenic analysis, for example. In addition, there are such other examples for the present invention as Chlamydomonas reinhardtii, Chlamydomonas moewusii, Chlamydomonas eugametos and Chlamydomonas segnis which belong to Chlamydomonas, Scenedesmus obliquus which belongs to Scenedesmus, Stichococcus ampliformis which belongs to Stichococcus, Nannochloris bacillaris which belongs to Nannochloris and Desmodesmus subspicatus which belongs to Desmodesmus. [OOl8l Furthermore, an example of Prasinophyceae for the present invention is l'etraselmis and examples of Bacillasiophyceae for the present invention are Cyanidioschyzon, Cyanidium, Galdieria and Porphyridium. It should be noted that any microorganism may be used. for the present invention as long as it is capable of producing oils, fats and carbohydrates, storing them in ils cells and converting the stored carbohydrates to oils and fats and that the microorganisms to be used for the present invention should not be restri.ct,ed to those above mentioned. Lo0191 Though culturing photosynthetic microorganisms in the culturing process Sl may be carried out in the atmosphere, it is preferable to intentiondlly introduce the carbon-dioxide gas in the culture solution to increase the con.centration ofthe carbon-dioxide dissolved in the culture solution to a higher one than the one for the condition in which the culture solution is just exposed to the atmosphere and increase the amount of oils, fats and carbohydrates that a+e produced through photosynthesis. For example, the carbon-dioxide gas discharged from factories and burning facilities may be .used to intentionally introduce the carbon-dioxide gas in the culture solutjon. If the discharged carbon-dioxide gas is used, it is better to remove dust particles, NOx and SOX included in the exhaust gas through a dust collector, a denitration apparatus and ' 1 a desulfuration apparatus in advance. 100201 A liquid depth of the culture solution in the culturing process Sl is preferably equal to or less than 50 cm. More preferably the liquid depth of the culture solution in the culturing process S1 is equal to or less than 30 cm. If the liquid depth is kept as above mentioned, the culture solution is well stirred in the up-down direction (the upper liquid is frequently replaced with the lower liquid) when the photosynthetic microorganisms proliferate as the - culturing progresses and the photosynthesis is being performed efficiently. tooz1l When Euglena are used as the photosynthetic microorganism, a culture medium to which a nitrogen source, a phosphorus source and minerals are added, such as a modified Cramer-Myers culture medium (including (NHd32HP04 l.Og/L, KF12P04 l.Og/L, MgS04-7Hz0 0.2glL, CaC12-2H20 0.02gL, Fez(S02)s-7HzO 3 mgL, MnClz.4HzO 1.8 mg/L, CoS04-7H20 1.5 mg/L, ZnS04-7820 0.4 mg/L, Na~Mo04 21120 0.2 u g L , CuS04-5H20 0.02 g/L, thiamin chloride(vitamin 131) 0.1 mg/L, and cya11ocobalamin(vitami~12)a nd with pU13.5 ) may be used. Any of (NHd32flS04. and NRyaq may be used instead of (NIId32flPO.t Any culture medium may be used for the present invention if it is fit for the photosynthetic microorganism tobe used. The culture medium is not limited to the above mentioned one. to0221 A pII of the culture solution is preferably between 2 and 6 and more preferably bel;ween 2 and 4.5. If the culture solution is acidic with. its ph value kept as indicated above, the photosynthetic microorganism is capable of proliferating so well as to prevail over the other organisms, which enable suppressing contamination. As a result, a batch culture method as well as a continuous culture method may be used for the present invention. LO0231 The pH of the culture solution is appropriately adjusted with powder reagents and reagent solutions. One of the power reagents used for this pH adjustment may be sodium bicarbonate, and such acidic solutions as a sulfuric acid solution and an acetic acid solution and such a basic solution as a sodium hydroxide solution are examples of the reagent solutions. [OOZd The oil and fat conversion step 52 that follows the step S1 is a step to convert the carbohydrates stored in cells of the photosynthetic microorganisms which have been cultured in the culture solution to oils and fats. The photosynthetic microorganisms stores oils and fats in their cells for self-defense when kept under anaerobic condition. Therefore, one of the methods for converting the carbohydrates stored in the photosynthetic microorganisms is, for example, to keep the culture solution in which the photosynthetic microorganisms have been cultured under anaerobic condition. In the present invention the anaerobic atmosphere refers to an atmosphere where there is no oxygen or less oxygen than in the atmosphere. The anaerobic co:ndition is created according the following process. After the photosynthetic ~.hicroorganis~anrse condensed in a condensation process SX2 (sedimentation condensation and centrifugal separation), the photosynthetic microorganisms are I;.ept in a closed space such as a closed container and a pipe, into which neither light nor oxygen comes, to prevent the photosynthetic microorganisms Gom producing oxygen through photosynthesis. 'Then oxygen in the closed space is consumed by the photosynthetic microorganisms respiration. As a result, the anaerobic condition is created. There may be other processes to create the anaerobic condition and the process to create the anaerobic condition is not .ii.:lnitedt. o the above mentioned one. For example, the anaerobic condition is ,-... .* .~. ,. a t e dby purging a space in which the photosynthetic microorganisms are kept 1.3 ith an inert gas like argon t;o rernove oxygen. i00251 In the present invention the condensation process S12 is preferably (xirried out before the oil and fat conversion process S2, that is, between the oil :;nd f:lt conversion process S2 and the culturing process S1. During the condensation process S12, the photosynthetic microorganism, in whose cells oils, fats and carbohydrates are stored, are condensed and part of the culture solution that is not needed is removed. As a result, the subsequent process is easily carried out and this condensation process S12 enables creating the anaerobic atmosphere earlier because the oxygen dissolved in the condensed culture solution is completely consumed earlier through the photosynthesis by the photosynthetic microorganisms owing to the less culture solution left after the part of the culture solution is removed. Furthermore, the oil and fat conversion process progresses -f aster. LO0261 In the condensation process 512, the culture solution is condensed preferably through the sedimentation condensation followed by the centrifugal separation process. Here the sedimentation condensation refers to having photosynthetic microorganism condensed through a natural sedimentation process such as leaving a solution inclusive of the photosynthetic microorganisms for 6 to 36 hours. Condensation through the centrifugal separation process is to have photosynthetic microorganisms condensed by a centrifugal force on a centrifugal machine. Performing the centrifugal separation process on a solution inclusive of photosynthetic microorganisms, the solution is separated into a heavy solution with a higher specific gravity inclusive of the photosynthetic microorganisms and a light so1ut;ion of a supernatant liquid with a lower specific gravity. Then by removing the light solution, the photosynthetic microorganisms co*itained in the heavy soluLion are appropriately condensed. Preferably as large an amount of the light solution as pc>ssibl.e is removed. The centrifugal separa1;ion process may be performed with a centrifugal acceleration between 5.000g and 20.000g. [0027] Both the supernatant liquid obtained after the sedimentation condensation and the light solution obtained after the centrifugal separation process may be put back into the culture solutio~tio be used in the culturing step 7 S1 and mixed with the culture solution. Thus the culture solution is more efficientIy used. In addition the photosynthetic microorganisms which are contained in the supernatant Liquid and the light solution and removed without being condensed are cultured again. E00281 The photosynthetic microorganisms are kept at 25 to 40 degree centigrade with no light radiated on the photosynthetic microorganisms in the oil and fat conversion process S2. When the culture solution is kept under the conditioned above mentioned, carbohydrates stored in the cells are - to be conve&d to oils and fats. [00291 The extraction process S3 to be subsequently performed is to extract oils and fats from inside the cells of the photosynthetic microorganisms. Oils and fats in the cells are to be extracted, for example, by a solvent extraction method with an organic solvent or a supercritical COz extraction method. An organic solvent which may be used for the solvent extraction method. is, for example, hexane. Since the hexane used for extracting oils and fats is reused for the solvent extraction afler being distilled, this process has an advantage wibh respect to the environment and the production cost. Coosol Such oils and fats as triglyceride and ester compo11nd.s (wax ester) made of a long chain fatty acid and a higher alcohol with one or two hydroxyl groups are extracted in this process. I00311 It is preferable to perform a drying process 522 between the oil and fat con.versiou process 52 and the extraction process S3. In this drying process S22, the culture solution that contains the cultured photosmthetic lnicroorganisms is dried. Since water which blocks the extraction is removed, oils and fats are more efficiently extracted after the drying process is performed. 100321 Nthough the culture solution may be drled in the drying process through 1 -- - ", sun-dry, heated air dry or freeze dqing method, it is preferable to make use of waste heat in exfiaust gas or vapor discharged &om factories or incineration plants. The culture solution is sufficiently dried without a need of other energies which work more rapidly and securely. Coo331 It is preferable to perform a collection process S32 after the extraction process 53. In the collection process S32 the photosynthetic microorganisms out of which oils and fats have been extracted in the extraction process S3 and which are defatted. The 'defatted photosynthetic microorganisms contain - proteins that constitute cells and pigment components and are utilized for biomass raw materials such as feed, fertilizer, solid fuel and raw materials for chemical products. COO341 A reforming process S4, in which the extracted oils and fats are modified, is subsequently performed. Reforming oils and fats corresponds to a reduction process such as a hydrogenation reaction. For example, a biofuel such as gas oil and jet fuel is produced by removing oxygen in wax esters through the hydrogenation reaction. [0035] Next, an embodiment of a biofiiel production apparatus of the present invention is explained with reference to Pig.2. The biofuel production apparatus shown in Fig.2 cor:responds to an example of a preferable embodiment of the production method for biofuel of the present invention. [00361 As shown in Fig.2, a biof~iepl roduct;ion apparatus A i~lcludesa culturing means I., a. condensing means 12 to condense the culture solution through sedimentation condensation and centrifugal condensation, an oil and fat converting means 2, a drying means 22 and an extraction means 4. Each of these means corresponds to a process of the similar name. 'I'herefore an explanation is skipped on significance, an act and an effect of each of these means which have been already explained m the description on the corresponding process. . . Lo0371 . The culturing means 1 may be, for example, a culture apparatus. The culture solution which is prepared for culturing photosynthetic microorganisms is put in the culture apparatus. In this embodiment, the culturing means 1 may be equipped with a carbon-dioxide gas supply means (not showd to intentionally introduce into the culture apparatus carbon-dioxide gas which is a carbon source, a pH keeping means 14 to keep the pH of the culture solution between 2 and 6 in the acid region and a nutritious salt supply means (not shown) show-n ) to supply nutritious salts to the culturing solution. 100381 The culturing process may be performed with the culture apparatus exposed to the atmosphere without any cover to cover an upper side of the culture apparatus. If the cover is attached over the culture apparatus, it is preferable to have a light transmission means (not showd through which sun light or illumination light transmits. The photosynthesis reaction progresses with the illurnillation light being radiated. Making use of the light transmission means and/or an illumination device, it is possible to have the photosynt;hesis of the photosynthetic microorganisms progress. 100391 When a carbon-dioxide gas supply means is used, it is preferable to use an airtight culture apparatus in order not to have the carbon-dioxide gas leak. I11 addition, the culture apparatus may be eq~ppedw ith a temperature serisor to measure a t,emperature of the culture solution or an inside of the cu1t;ure apparatus, a light intensity sensor, a gas concentration sensor to 11.reasure a oxygen concentration or a carbon-dioxide concentration, a temperature co~trol device to keep the temperature of the culture solution at a constant temperature and a stirring device to stir the culture solution (none of them showd. 100401 A condensation means 12 to perform the sedimentation condensation is, for example, a sedimentation tank. When the sedimentation tank is cLisposed . - 9 with an upper solution level in the sedimentation tank being higher than an q p e r solu6on level in the culture apparatus, the supernatant liquid in the sedimentation tank is transferred back into the culture apparatus due to a difference in the solution level between the sedimentation tank and the culture apparatus. The transferred supernatant liquid contains nutritious components and these nutritious components are reused and not wasted by transferring back the supernatant Liquid. [00411 A condensation means for the centrifugal separation process may - be, for example, a cent

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1 10228-DELNP-2012-RELEVANT DOCUMENTS [16-03-2020(online)].pdf 2020-03-16
1 Priority Document.pdf 2013-01-12
2 10228-DELNP-2012-IntimationOfGrant19-02-2019.pdf 2019-02-19
2 Form-5.pdf 2013-01-12
3 Form-3.pdf 2013-01-12
3 10228-DELNP-2012-PatentCertificate19-02-2019.pdf 2019-02-19
4 Form-1.pdf 2013-01-12
4 10228-DELNP-2012-Written submissions and relevant documents (MANDATORY) [30-11-2018(online)].pdf 2018-11-30
5 10228-delnp-2012-GPA-(22-01-2013).pdf 2013-01-22
5 10228-delnp-2012-ExtendedHearingNoticeLetter_20Nov2018.pdf 2018-11-02
6 10228-delnp-2012-Correspondence-Others-(22-01-2013).pdf 2013-01-22
6 10228-DELNP-2012-Correspondence to notify the Controller (Mandatory) [03-10-2018(online)].pdf 2018-10-03
7 10228-delnp-2012-Form-3-(17-05-2013).pdf 2013-05-17
7 10228-DELNP-2012-FORM 3 [20-09-2018(online)].pdf 2018-09-20
8 10228-DELNP-2012-HearingNoticeLetter.pdf 2018-09-03
8 10228-delnp-2012-Correspondence Others-(17-05-2013).pdf 2013-05-17
9 10228-DELNP-2012-ABSTRACT [31-10-2017(online)].pdf 2017-10-31
9 10228-delnp-2012-Correspondence-Others-(28-05-2013).pdf 2013-05-28
10 10228-DELNP-2012-CLAIMS [31-10-2017(online)].pdf 2017-10-31
10 10228-DELNP-2012.pdf 2016-03-13
11 10228-DELNP-2012-FER.pdf 2017-08-28
11 10228-DELNP-2012-FER_SER_REPLY [31-10-2017(online)].pdf 2017-10-31
12 10228-DELNP-2012-OTHERS [31-10-2017(online)].pdf 2017-10-31
12 10228-DELNP-2012-PETITION UNDER RULE 137 [27-10-2017(online)].pdf 2017-10-27
13 10228-DELNP-2012-FORM 3 [27-10-2017(online)].pdf 2017-10-27
14 10228-DELNP-2012-OTHERS [31-10-2017(online)].pdf 2017-10-31
14 10228-DELNP-2012-PETITION UNDER RULE 137 [27-10-2017(online)].pdf 2017-10-27
15 10228-DELNP-2012-FER.pdf 2017-08-28
15 10228-DELNP-2012-FER_SER_REPLY [31-10-2017(online)].pdf 2017-10-31
16 10228-DELNP-2012-CLAIMS [31-10-2017(online)].pdf 2017-10-31
16 10228-DELNP-2012.pdf 2016-03-13
17 10228-delnp-2012-Correspondence-Others-(28-05-2013).pdf 2013-05-28
17 10228-DELNP-2012-ABSTRACT [31-10-2017(online)].pdf 2017-10-31
18 10228-delnp-2012-Correspondence Others-(17-05-2013).pdf 2013-05-17
18 10228-DELNP-2012-HearingNoticeLetter.pdf 2018-09-03
19 10228-delnp-2012-Form-3-(17-05-2013).pdf 2013-05-17
19 10228-DELNP-2012-FORM 3 [20-09-2018(online)].pdf 2018-09-20
20 10228-delnp-2012-Correspondence-Others-(22-01-2013).pdf 2013-01-22
20 10228-DELNP-2012-Correspondence to notify the Controller (Mandatory) [03-10-2018(online)].pdf 2018-10-03
21 10228-delnp-2012-GPA-(22-01-2013).pdf 2013-01-22
21 10228-delnp-2012-ExtendedHearingNoticeLetter_20Nov2018.pdf 2018-11-02
22 Form-1.pdf 2013-01-12
22 10228-DELNP-2012-Written submissions and relevant documents (MANDATORY) [30-11-2018(online)].pdf 2018-11-30
23 Form-3.pdf 2013-01-12
23 10228-DELNP-2012-PatentCertificate19-02-2019.pdf 2019-02-19
24 Form-5.pdf 2013-01-12
24 10228-DELNP-2012-IntimationOfGrant19-02-2019.pdf 2019-02-19
25 10228-DELNP-2012-RELEVANT DOCUMENTS [16-03-2020(online)].pdf 2020-03-16
25 Priority Document.pdf 2013-01-12

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