Abstract: The present invention provides a protein-immobilized electrode which is stably used for long time and a method of manufacturing the same in which cytochrome c552 having high stability is immobilized to a chemically-stable gold electrode while maintaining electron transfer capability of the cytochrome c552. A self-assembled monolayer 12 is formed on a gold electrode 11 by using hydrophobic thiol and hydrophilic thiol. By dipping the gold electrode 11 on which the self-assembled monolayer 12 is formed in a cytochrome c552 solution, a protein-immobilized electrode in which a cytochrome c552 13 is immobilized to the gold electrode 11 with the self-assembled monolayer 12 in between is produced.
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