. The method of claim 2, wherein the exoglycosidase is Sialidase which has the ability to cleave terminal sialic acid from both N- and O-linked glycans of glycoproteins.
The method of claim 14, wherein the enzymatic activity is completed in 30 minutes.
The method of claim 1, wherein the released glycans are separated by liquid chromatography.
The method of claim 1, wherein the glycoprotein is an antibody, fragment thereof or fusion protein with multiple glycan sites.
A method of partial de-glycosylation of a glycoprotein for comparing tertiary structure, wherein, the method comprising steps of:
(a) providing a glycoprotein;
(b) combining the glycoprotein with endoglycosidase to partially cleave N-linked
glycans in an amount from 1 unit to 10 units per 1 mg of glycoprotein;
(c) incubating components of step (b) at a temperature from about 37°C for about
45 mins to 8 hrs to provide for a partially de-glycosylated protein.
The method of claim 18 wherein the endoglycosidase is PNGaseF which has the ability to cleave N-linked glycans from glycoproteins when the innermost residue is GlcNAc.
The method of claim 18, wherein the partially de-glycosylated glycoprotein is analysed using capillary electrophoresis.
The method of claim 18, wherein the glycoprotein is an antibody, fragment thereof or fusion protein with multiple glycan sites.