DESC:FIELD OF THE INVENTION
The present invention relates to the field of reaction biology product for agriculture
and horticulture. More particularly, it relates to a formulation comprising of one or more plants. Particularly plants to provide insecticide, pesticide, growth regulator and thus help agriculture, horticulture crops and plants.

BACKGROUND OF THE INVENTION
Fertilizer’s usage in the recent times has come down with the raise of dead zones and increasing pest population. Many herbs are used as a fertilizer, plants requirement are met by unique products in plant kingdom which enrich growth, protect plants from pest and disease by indole acetic acid, indole butyric acid and also provide unique bio enzyme products.

“Double-blinded randomized controlled trial for immunomodulatory effects of Tulsi (Ocimum sanctum linn) leaf extract on healthy volunteers” Tulsi (Ocimum sanctum linn.) is considered as a sacred herb and traditionally it is believed that consumption of Tulsi leaf on empty stomach increases immunity. Experimental studies have shown that alcoholic extract of Tulsi modulates immunity. The present study was designed to evaluate the immunomodulatory effects of ethanolic extract of Tulsi leaves through a double-blinded randomized controlled cross-over trial on healthy volunteers. Three hundred milligrams capsules of ethanolic extracts of leaves of Tulsi or Placebo were administered to 24 healthy volunteers on empty stomach and the results of 22 subjects who completed the study were analyzed. The primary objective was to study the levels of Th1 and Th2 cytokines (interferon-? and interleukin-4) during both pre and post intervention period in blood culture supernatants following stimulation with lipopolysaccharide and phytohaemagglutinin. Other immunological parameters such as T-helper and T-cytotoxic cells, B-cells and NK-cells also were analyzed using flowcytometry. Statistically significant increase in the levels of IFN-? (p=0.039), IL-4 (p=0.001) and percentages of T-helper cells (p=0.001) and NK-cells (p=0.017) were observed after 4 weeks in the tulsi extract intervention group in contrast to the placebo group. These observations clearly ascertain the immunomodulatory role of Tulsi leaves extract on healthy volunteers.

“In vitro antimicrobial activity of flavanoids of Ocimum sanctum with synergistic effect of their combined form” objective to evaluate the antibacterial activity of flavanoids, orientin and vicenin, obtained from leaves of Ocimum sanctum, have also been compared by their combine sample. Methods aqueous extract of fresh leaves of ocimum sanctum was assessed for the isolation and purification of different flavanoids. The antibacterial activity of orientin, vicenin and combine sample of both these flavanoids was evaluated according to well diffusion method against some bacteria causing urinary tract infection (UTI) in human results. The result indicated that the combined sample or synergistic activity of both individual flavanoids showed positive result against escherichia coli, proteus, staphylococcus aureus, staphylococcus cohni and klebsialla pneumonia with zone of inhibition 20.12, 20.75, 20.95, 19.55 and 20.1 mm at concentration of 400 mg/ml respectively. But the individual flavanoids showed the positive result against only limited microorganism. Conclusions the finding of the present study provides the evidence that this flavanoid sample is used as an antibacterial agent. This is also beneficial to use this combine sample of different flavanoids of Ocimum sanctum for medication and other purposes.

“Enhancing chloramphenicol and trimethoprim in vitro activity by Ocimum sanctum Linn. (Lamiaceae) leaf extract against Salmonella enterica serovar Typhi” To evaluate the antibacterial activity of Ocimum sanctum (O. sanctum) leaf extract, alone, and in combination with chloramphenicol (C) and trimethoprim (Tm) against Salmonella enterica serovar Typhi (S. typhi).
“Anti-herpes simplex virus activity of extracts from the culinary herbs Ocimum sanctum l., Ocimum basilicum l. and Ocimum americanum l” his study demonstrates anti-herpes simplex virus activity of dichloromethane and methanol extracts of Ocimum sanctum l., Ocimum basilicum l. and Ocimum americanum l. Green monkey kidney cells were protected from HSV-2 infection by the dichloromethane extract of o. americanum l. and the methanol extract of o. sanctum, with therapeutic indexes (TI) of 1.865 and 1.644, respectively, when the cells were treated before viral infection. Herpes simplex virus-2 (HSV-2) infection was inhibited during viral adsorption when the cells were treated with methanol extracts of o. americanum l., o. sanctum l. and o. basilicum l. with ti of 2.345, 2.473 and 1.563, respectively, whereas dichloromethane extracts of o. americanum l. and o. basilicum l. resulted in TI of 2.623 and 1.835, respectively. The methanol extract of o. americanum l. and the dichloromethane extract of o. basilicum l. inhibited HSV-1F with TI of 1.63 and 2.215, respectively, after viral adsorption. The inhibitory effects of extracts on HSV-2G, after viral adsorption, were quite high, for the dichloromethane extract of o. sanctum l. and the methanol extract of o. sanctum l. with ti of 10.003 and 29.395, respectively. the inhibitory effect of the o. americanum l. extract on hsv-1f and hsv-2 yield, after viral replication, was highest 30 h after treatment. the reduction of viral titers by 8.0 and 10.8 folds was observed when cells were treated with dichloromethane and methanol extracts of o. americanum l. Moreover, time-dependent virucidal effects of the extract on viral particles were demonstrated, since direct inhibition of both HSV-1F and HSV-2G was shown by a reduction in the amount of plaques by 100%, after treatment with the dichloromethane and methanol extracts of o. americanum l. therefore, dichloromethane and methanol extracts of o. sanctum l., o. basilicum l. and o. americanum l. showed anti-hsv activities at various steps of the viral multiplication cycle.

OBJECTS OF INVENTION
The primary object of the present invention is to provide emulsifiable formulation for agriculture and horticulture using some plants.

it is another object of the present invention to comprising the following plants namely, Ocimum sanctum, Cynodon dactylon, Solanum trilobatum, Ficus religiosa and Thevetia neriifolia leaves.

It is another object of the present invention, wherein emulsifier is selected from ionic emulsifier.

It is another aspect of the present invention to provide a method of preparation of an emulsifiable concentrate formulation for agriculture and horticulture, comprising some of the steps.

it is another object of the present invention, wherein the herb parts are selected from whole plant of Ocimum sanctum, whole plant of Cynodon dactylon, fruits of Solanum trilobatum, leaves of Ficus religiosa, and leaves of Thevetia neriifolia.

It is another aspect of the present invention, wherein all the herbal ingredients are powered, mixed well and then ionic emulsifier is added to get a uniform emulsion concentrate.
It is another aspect of the present invention, wherein neither the ingredients of the formulation float or settle down.

SUMMARY OF THE INVENTION
One or more of the problems of the conventional prior art may be overcome by various embodiments of the present invention.

In one aspect of the invention, an emulsifiable formulation for agriculture and horticulture, comprising the following plants namely, Ocimum sanctum (25%), Cynodon dactylon (25%), Solanum trilobatum (10%), Ficus religiosa (15%) and Thevetia neriifolia (25%) leaves.

It is another object of the present invention to provide an emulsifiable concentrate formulation for agriculture and horticulture, wherein the ionic emulsifier is selected from polymeric base ionic surfactants, phosphonate base ionic surfactants and silicone base ionic surfactants.

It is another aspect of the present invention to provide a method of preparation of an emulsifiable concentrate formulation for agriculture and horticulture, comprising the steps: cleaning and using the whole plant, grinding the plant and obtain a wet product, mixing the grinded part and blend in an emulsifier to yield an emulsified concentrate, wherein the emulsified concentrate comprises by weight: Ocimum sanctum (25%), Cynodon dactylon (25%), Solanum trilobatum (10%), Ficus religiosa (15%), Thevetia neriifolia (25%) and ionic emulsifier- concentration.

it is another object of the present invention to provide and an emulsifiable concentrate formulation for agriculture and horticulture, wherein the herb parts are selected from whole plant of Ocimum sanctum, whole plant of Cynodon dactylon, seed of Saraca asoca, seed of Cassia fistula, fruits of Solanum trilobatum, leaves of Ficus religiosa, leaves of Thevetia neriifolia.

It is another aspect of the present invention to provide a method of preparation of an emulsifiable concentrate formulation for agriculture and horticulture, wherein all the herbal ingredients are powered, mixed well and then ionic emulsifier is added to get a uniform emulsion concentrate.

It is another aspect of the present invention to provide a method of preparation of an emulsifiable concentrate formulation for agriculture and horticulture, wherein neither the ingredients of the formulation float or settle down.

BRIEF DESCRIPTION OF DRAWINGS
In which the features, advantages and objects of the invention, as well as others which will become apparent, may be understood in more detail, more particular description of the invention briefly summarized above may be had by reference to the embodiment thereof which is illustrated in the appended drawings, which form a part of this specification. It is to be noted, however, that the drawings illustrate only a preferred embodiment of the invention and is therefore not to be considered limiting of the invention's scope as it may admit to other equally effective embodiments.

Figure 1 illustrates the flow chart, in accordance with an embodiment of the present invention.

BRIEF DESCRIPTION OF THE INVENTION
At the outset of the description that follows, it is to be understood that the ensuing description only illustrates a particular form of this invention. However, such a particular form is only an exemplary embodiment and is not intended to be taken restrictively to imply any limitation on the scope of the present invention.

The following specification particularly describes the invention and the manner in which it is to be performed, Ocimum sanctum (holy basil), Cynodon dactylon (scotch grass), Solanum trilobatum (purple fruited pea egg plant), Ficus religiosa (sacred fig), Thevetia neriifolia (yellow oleander), to feed plants and survive also- unique reaction biology product.

Two herbs that are Ocimum sanction and Bermuda grass are unique for host plant where we use will get enough enzymes and essential oils which uniquely help plants in multi ways, both will protect from pest and disease. Another important point is to get indole acetic acid for growth.

Another point they will get indole butyric acid for growth of roots. Bermuda grass (Arugam grass) will keep flumes free from pest and disease. It will boost photo synthesis since travelling through phloem and in chlorophyll a & b there by photosynthesis increased.
Thevesia nurifolia will keep away any pest and disease and there by triple help other three plants will strength the host.

The leaves and seeds of Ocimum sanctum possess several hormones and essential oils ant it absorbs indole acetic acid to help plants for growth and prevents pest and disease, in short it saves the plant when applied at 2ml per litre emulsifiable concentrate. Uniquely helps adjacent crops also.

Bermuda grasses love to travel in phloem and reach chlorophyll compound a & b and it increase photosynthesis. By travelling the phloem keeps the plant healthy and free from pest and disease and also strengthens the plants. Thevetia neerifolia leaves also control the pests and disease.

Solanum trilobatum(Thoothuvalai and Saraca etc) will give abnormal strength to plants where we apply the product.

“REACTION BIOLOGY”
Important plants and product for reaction biology based patent.
The present invention relates to plant of various genuses to help agriculture and horticulture crops and plants. This unique invention is to help plants from pest & disease and also feed immunity through phloem. This dual function is through Reaction Biology.
This product can be used in all crops including poly house cultivation. In poly house this will do both the job that is pest control and feeding.

The modern world & science is typically coming round to biology in respect of medicine, agriculture & horticulture. This product is useful for plants to synthesize their requirements of plant products like enzymes, protein & other vital needs for production and helps for photosynthesis.

The Patent with heterocyclic products helps plants to synthesize their requirements.
Now let us see the plants selected for REACTION BIOLOGY

1. Ocimum sanctum (HOLY BASIL)
This herb is largely cultivated and supplied to hanuman temples. We cannot see any pest in Tulasi supplied to temples. In a way, have seen where they are cultivated, it is able to help nearby crop also from Pest. In the patent we see biology impact as informed by “Nature” published on Jan 14th. Our product with all combination at 2.5 to 3 gms emulsifiable concentrate at one litre of water will eliminate or prophylactically safeguard plants from pest attack. It is really a wonder how these plants are free from pest when we see in temple shops.

No insect pest or disease is seen in the plants. When it is nearer to a cultivar pest damage is minimum or even nil. It contains vitamin C, A and minerals.
Ocimum sanctum contains essential oils and that will help attract fertilizer and secure fertilizer from soil as well as atmosphere. The essential oils and hormones, indole butyric acid and indole acetic acid help to absorb fertilizer from soil and atmosphere.

2. Cynodon dactylon (SCOTCH GRASS)
In the same way Cynodon dactylon never allow any pest near and in fact drive away if any comes in touch. This grass also sold in all ganesha temples and you can see pest free in every temple shop. This grass is collected and supplied for temples in a clean way without any insect.

No pest seen in this plant. Naturally free from pest & diseases. It is used as drug to clean food duct. When it flows in phloem no pest will occur. It is a function of root biomass, morphology, ability to explore regions with abundant nutrients and physiological capacity for nutrient uptake.

For the above reasons, this invention uses two plant extracts Ocimum and Cynodon to do the job by protecting plants against pest and diseases.

3. Saraca asoca (ASHOKA TREE)
The seeds available in plants are free from pest and disease and its use helps plants to live strongly when in phloem. Ashoka bark is rich in tannins, haematoxylin, flavonoids, steroids, saponins, volatile oil, glucosides, cacethol, carbonic, calcium, iron, ketosterol and various steroidal glycosides.

4. Cassia fistula (GOLDEN SHOWER TREE)
This plant mostly free from pest & disease. It contains ascorbic acid (15.3%), glutenic (13%) and lysine (3.3%).

5. Solanum trilobatum (PURPLE FRUITED PEA EGGPLANT)
It is full of thorn and prevents pest. It is used by Vallalar (Saint) and lived healthily without any other food. Here we should use its extraction to create immunity in plants. It contains proteins (5.5), carbohydrates (25%), ash (28.6%) and fibre (3.3 %) and all in 100 kg.

6. Ficus religiosa (SACRED FIG)
Mostly free from pests. This seed with germinate anywhere even in concrete surface. It is used mainly to strengthen the end product. It contains phyto-constituents, like sitosterol-D-glucoside, vitamin K.

7. Thevetia neriifolia:
Thevetia neriifolia leaves will enhance contact stomach poison an insects will disappear on feeling the smell itself. Thevetia neriifolia is high poisonous and control pest as contact and stomach poison. It imparts pesticidal activity.
The composition comprises predominantly:
1. Five herbals (1 to 5) does the desired result i.e. pest management, growth & yield.
2. Herbals are unique reaction biology product to help plant kingdom.
3. A unique product to help plant kingdom for bio feed and bio protect. ,CLAIMS:WE CLAIM:
1. An emulsifiable concentrate formulation for agriculture and horticulture, comprising:
Ocimum sanctum– 25%;
Cynodon dactylon – 25%;
Solanum trilobatum – 10%;
Ficus religiosa – 15%;
Thevetia neriifolia –25%; and
emulsifier (ionic) – q.s (quantum sufficient according to total product).

2. An emulsifiable concentrate formulation for agriculture and horticulture as claimed in claim 1, wherein the ionic emulsifier is selected from polymeric base ionic surfactants, phosphonate base ionic surfactants, silicone base ionic surfactants.

3. A method of preparation of an emulsifiable concentrate formulation for agriculture and horticulture, comprising the steps of:
cleaning and using the whole plant;
grinding the plant and obtain a wet product; and
mixing the grinded part and blend in an emulsifier to yield an emulsified
concentrate;
wherein the emulsified concentrate comprises by weight:
Ocimum sanctum – 25%;
Cynodon dactylon – 25%;
Solanum trilobatum – 10%;
Ficus religiosa – 15%;
Thevetia neriifolia – 25%; and
ionic emulsifier- concentration.

4. An emulsifiable concentrate formulation for agriculture and horticulture as claimed in claim 1, wherein the herb parts are selected from whole plant of ocimum sanctum, whole plant of Cynodon dactylon, fruits of Solanum trilobatum, leaves of Ficus religiosa, and leaves of Thevetia neriifolia.

5. An emulsifiable concentrate formulation for agriculture and horticulture as claimed in claim 1, wherein all the herbal ingredients are, grinded in mixer grinder (Mixi) and emulsifiable concentrate well and then ionic emulsifier is added to get a uniform emulsion concentrate.

6. An emulsifiable concentrate formulation for agriculture and horticulture as claimed claim 1, wherein neither the ingredients of the formulation float or settle down.