FORM 2
THE PATENTS ACT 1970
(39 of 1970)
&
The Patent Rules 2003
COMPLETE SPECIFICATION
(sec sections 10 & rule 13)
1. TITLE OF THE INVENTION
"SAFE, BREATHABLE ANTI-VIRAL AND ANTI-BACTERIAL BARRIER"

2. APPLICANT (S)
NAME NATIONALITY ADDRESS
1PSUM
LIFESCIENCES
LLP INDIAN 217, CHAMPAKLAL INDUSTRIAL ESTATE, NR. SION TELEPHONE EXCHANGE, SION -EAST, MUMBAI - 400 022, MAHARSHATRA, INDIA.
3. PREAMBLE TO THE DESCRIPTION
COMPLETE SPECIFICATION The following specification particularly describes the invention and the manner
in which it is to be performed

Field of invention
The present invention relates to a safe, breathable anti-viral and anti-bacterial barrier in form of face mask or respirator, which acts as the first line of defence against fatal viruses and bacteria. The barrier designed to protect individuals from viral and bacterial infections. Furthermore, it relates to killing/inactivating agent that kills the adsorbed pathogens, so after use upon discard of barrier it prevents the spread of the viruses and bacteria.
Background of the invention
Respiratory infection is the major cause of death among all infectious diseases caused by various infectious viruses and bacteria. For a few diseases there is a treatment available, however, in case of disease that are difficult to treat, prevention is the only solution.
In the case of respiratory infectious diseases, the prevention of contact of disease carriers is a challenge. For major of identified diseases; vaccines and/or drugs are available to stop the infection and/or to.treat infected personnel and life can be saved. However, this is a post-infection option, and it is costly. If the population is aware of the means of prevention of spreading infectious disease then more life can be saved and protected in the easiest way.
In case of respiratory infections, means of preventions are: 1) isolation area, 2) quarantine of the infected material, 3) use of special masks or other personal protective equipment (PPE) by the medical practitioner. However, the use of special masks or PPE is very rare and limited by the public, which makes disease outbreak more serious. The use of PPE, respirators are very limited because of its own limitations like affordability, easy to handle, breathability thus wide acceptance.
For non-professional public use the masks or respirator should have/be (1) affordable; (2) easy to use-breathable; (3) give broad-spectrum protection; (4) prevent the spread either by just avoiding contact or avoiding the contact followed by killing; (5) should have broad spectrum activity (to cater other infectious carriers also); (6) non-contaminant post usage if some accidental release happens after

usage; (7) not the reason for potential biomedical waste (the PPE, which just prevent the infectious agents and do not kill it are the potential biomedical waste and source for next spread); (8) non-toxic/non-harmful to user, and (9) allowed for prolonged exposure and usage.
There are anti-viral masks available, which works on the principle of "filtration" alone or "filtration" followed by "killing" using a potential anti-viral agent. However, it has the limitations as given below:
Masks working on the principle of Virus "filtration" are generally; of low pore size i.e. 0.2-μm, which do not allow the virus to penetrate the barrier and thus the protection. However, these masks have limitations in terms of ease of usage -breathability, prolonged usage. It just prevents the infectious viruses and/or bacteria but do not kill it. Due to non-killing nature, it also become the potential biomedical waste, enriched in infectious viruses and/or bacteria.
According to researchers, a virus can live on a mask for up to a week. However, incorrect use moreover, disposal may actually increase the risk of pathogen transmission, rather than reduce it, especially when non-professionals (public) uses masks. If a mask is not handled carefully - say one touch the mask himself, wear it for way too long, put it in his pocket, or don't wash hands after - the virus can be passed on to someone else. Most of the low-pore respirators like N95/N90 are having issues on breathability, and to solve it one need to add different category of the exhalation valves that again make it non-affordable for public use and their usages are limited to medical practitioner.
However, masks working on the principle of virus "filtration" as well as "killing" are again low pore size i.e. 0.2 μm, which again do not allow the virus to penetrate the barrier as well as it comprises the potential virus killing agent. These kind of masks solve the problem of potential biomedical waste and further spreading of the virus. However, it has the same limitations on ease of use as mentioned above for low-pore respirators like N95/90 leading to issues on breathability. Additionally, these kind of masks have limitations for selectivity associated with the kind of (virucidal/bactericidal and/or narrow - spectrum) viral killing agent. Toxicity
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associated with the prolonged usage of these kind of masks again depend upon the type of killing agent.
If porosity is increase in these masks then, it achieves the breathability, but due to bigger pore structure, the filtration efficiency is lost, and since the pores are bigger, anti-viral and/or anti-bacterial agents cannot come in contact to the infectious agents surface, and killing timing and efficiency is also hampered. So achieving breathability and maintaining filtration + killing efficiency is a challenge.
Following are the prior art that describes different type of facemask available in public domain.
Choi et al (https://www.huffingtonpost.ca/entry/coronavirus-face-mask-alberta-Scientists_ca_5e3cl544c5b6bb0ffc0c2ecd) discloses use of coating of higher concentration of sodium chloride salts, which works on the principle of "filtration followed by killing" using the hyperosmotic solution of salt (i.e. sodium chloride). However, this requires virus to be exposed to the salt crystals that poke into virus structure and kills it. This mechanism have limitations in terms of specificity of virus killing, contact timing, as well as contact points of virus on the masks.
WO2016125173A1, WO2008.145175A1 and US10201198B2 describes use of antimicrobial fabric materials for use in safety masks as well as personnel protection clothing, which relates to a polymeric material incorporated therein a synergistic combination of at least two metal oxide powder, wherein powder comprises zinc oxide, and staffnic oxide, zirconium dioxide as a killing agents. However, these chemical killing agents do not show compatibility with the wearer and their toxic effects prevents prolonged usage.
CN106388081B describes use of zinc oxide quantum dots to be used as a killing agent in the mask. However, as mentioned above these masks have toxicity due to the killing agent and do not allow the prolonged usage.
CN1799437A describes a health-increasing mask containing the ingredients from the natural flower, which slowly goes into the body of the wearer and helps in improving the immunity of wearer that ultimately lead to protection by infectious

diseases. However, this method is indirect method, and have limitations in terms of the immunity development by the wearer, as its effect varies from person to person.
CN201758804U describes use of antimicrobial fabric where vinegar is used as a killing agent. However, there are limitations in this mechanism as vinegar is narrow-spectrum to kill viruses and bacteria. This disclosure also do not describe about the use the killing mechanism, as the weak effect of the acid cannot kill the viruses very quickly upon the contact.
JPH1119238A describes masks having the antimicrobial effects where silver is used as the killing agent. However, the use of silver as a killing agent makes the product costly and non-affordable for general public use, and contacting the infectious organisms with the killing agents is the challenge when the breathable mask is to be made.
CN103726224A describes the masks. The mask is made up of zein, a functional protein, where this protein is claimed to be antimicrobial, and is used as important polymer for making non-woven fabric. This makes a safe, breathable mask, but the antimicrobial activity is limited and narrow spectrum, in addition to speed of killing the organisms. There is need of improvement in this product for better and general public usage.
While considering the all the problem associated with prior art, there is a need to develop safe, affordable protection devices which is breathable, works on the principle of Filtration + Killing, with broad spectrum anti-bacterial and anti-viral activities available to public to be used for not only to prevent the spread of the viral and/or bacterial infections but also kills the pathogens.
Objects of present invention
The main object of the present invention is to provide the better, affordable and safe product, for first line of defence against harmful enveloped virus and bacterial
infections.
Another object of the present invention is to provide inactivating/killing agent which kills the micro-organisms very quickly, so that upon removal of the barrier,
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there is no chances of further spreading/propagation of attached viruses and bacteria.
Another object of the present invention is to provide breathable wide-pore barrier, which is as efficient for maintenance of containment from infectious/non-infectious micro-organisms as low-pore filters does.
Summary of the present invention
One of the aspects of the present invention is to provide a safe, breathable anti-viral and anti-bacterial barrier for preventing micro-organisms to pass through, the barrier comprising:
a) a solid breathable porous support material/s; and
b) a complex of lipophilic, anti-oxidant, anti-bacterial and anti-viral agent(s) responsible for adsorption of microorganisms and bringing it in next to killing agent present in the complex.
wherein the solid breathable porous support material/s is any natural and/or non-natural breathable porous matrix having pore size in the range of 0.4 urn to 0.7 urn;
wherein the complex of lipophilic, anti-oxidant, anti-viral and anti¬bacterial agent is a Tocopherol-Curcumin complex, which is responsible for adsorption of microorganisms and bringing it next to killing agent present in the complex.
Brief description of the Figures
Figure 1 shows Fabric without any treatment, wherein 0.4 μm Raw fabric when taken, it shows definite porosity, which acts as a solid support for the barrier.
Figure 2 illustrates Barrier with 0.4 μm fabric -Tocopherol depleted - Curcumin Coating, wherein 0.4 μm raw fabric when coated with Tocopherol depleted -curcumin. As indicated, curcumin mixture, being in smaller structure, when coated onto the porous fabric, it just remains with the fibres, and the microbes being larger in size do not come in contact with the killing agent.

Figure 3 depicts Barrier with 0.4 urn fabric -Curcumin depleted - Tocopherol Coating, wherein 0.4 μm raw fabric when coated with Curcumin depleted -Tocopherol. As indicated, Tocopherols, which is oily in nature and bulky, when coated onto the porous fabric, it decreases the pores of the fabric, and the microbes being larger in size come in contact with Tocopherol. Most of the infectious microorganisms contains lipid layer at the outer-side, which is lipophilic. Tocopherol being lipophilic in nature, adsorb these microorganisms and creates the trap, via hydrophobic-hydrophobic interactions. However these decreased porosity will not have impact on the breathability (differential pressure) as indicated in example 5.
Figure 4 shows Barrier with 0.4 μm fabric - Tocopherol - Curcumin Complex Coating, wherein 0.4 μm raw fabric when coated with Tocopherol-Curcumin Complex. As indicated, Tocopherols, which is oily in nature and bulky, when coated onto the porous fabric, it decreases the pores of the fabric, and the microbes being larger in size come in contact with Tocopherol. Most of the infectious microorganisms contains lipid layer at the outer-side, which is lipophilic. Tocopherol being lipophilic in nature, adsorb these microorganisms and creates the trap, via hydrophobic-hydrophobic interactions. Now Curcumin present into Tocopherol-curcumin complex, being oil soluble will reach to the adsorbed micro¬organism, and will inactivate and/or kill the microorganism. However, this will not have any impact on the decrement of breathability (differential pressure).
Detailed description of the invention
In the present invention, numbers of terms are used for description of invention. The definitions of terms are as follows:
Definitions:
The term "facemask" OR "barrier" used herein refers to a device that creates a physical barrier between the mouth and nose of the wearer and potential contaminants in the immediate environment.
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Facemask protects the nose and mouth of wearer vapour drops carrying viruses or other infectious pathogens.
The term "respirator" used herein refers to a respiratory protective device designed to achieve a very close facial fit and very efficient filtration of airborne particles.
The terms "facemask", "respirator" and "barrier" can be used interchangeably in the specification.
The term "micro-organism" used herein refers to "a microscopic organism, especially a bacterium, virus and/or fungus.
The term "infectious micro-organism" OR "pathogens" used herein refers to a disease-causing micro-organism liable to the people, organisms etc. through the environment.
The terms "infectious micro-organism", "pathogens" can be used interchangeably in the specification.
The term "Anti-viral" used herein refers to an agent that kills a virus or that suppress its ability to replicate and hence, inhibits its capability to multiply and reproduce.
The term "Anti-bacterial" used herein refers to an agent that kills a bacterial or that suppress its ability to replicate and hence, inhibits its capability to multiply and reproduce.
The term "Tocopherol" used herein refer, to Tocopherol is obtained from alpha-Tocopherol and/or beta-Tocopherol and/or gamma-Tocopherol and/or delta-Tocopherol, its mixtures with or without the means,of extended shelf life either in native form or in encapsulated form.
The term "Curcumin" used herein refer to Curcumin and/or turmeric extract containing curcumin and/or curcuminoids mixtures comprising Curcumin, Demethoxy Curcumin, Bis-demethoxy Curcumin and/or turmeric oleoresin containing Turmeric and/or defatted turmeric oleoresin and/or mixtures there of used as a sources of curcumin, with or without the means of extended shelf life either in native form or encapsulated form.

The term "Tocopherol-Curcumin complex" OR "killing/deactivating compound/agent" used herein refer to a mixture which is combination of tocopherol and curcumin obtained either from natural sources or synthetic sources including derivatives like thereof. The ratio of tocopherol to curcumin in the mixture can be obtained including but not limited to 50:50, 60:40, 70:30, 80:20, 90:10, 40:60, 30:70,20:80, 10:90 etc.
The present invention relates to new product i.e. breathable anti-viral and anti¬bacterial barrier between the mouth and nose of the wearer and potential contaminants in the environment in form of facemask or respirator, which acts as the first line of defence against fatal viruses and bacteria. The fatal effects of viruses and bacteria are flu but not limited to other respiratory illness.
The present invention discloses a product which is breathable anti-viral and anti¬bacterial barrier as it works on the principle of adsorption followed by killing and inactivation of enveloped viruses and bacteria, and it need not require use of low pore barriers, making it easy, breathable barrier.
One of the embodiment of present invention provides a safe, breathable anti-viral and anti-bacterial barrier for preventing micro-organisms to pass through, the barrier comprising:
c) a solid breathable porous support material/s; and
d) a complex of lipophilic, anti-oxidant, anti-bacterial and anti-viral agent(s) which is responsible for adsorption of microorganisms and bringing it in next to inactivating/killing agent present in the complex.
Another embodiment of present invention provides a safe, breathable anti-viral and anti-bacterial barrier for preventing micro-organisms to pass through, wherein the solid porous support material is any natural and/or non-natural breathable porous matrix.
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Another embodiment of present invention provides a safe, breathable anti-viral and anti-bacterial barrier for preventing micro-organisms to pass through, wherein the solid porous support material is having pore size in the range of 0.4 urn to 0.7 urn.
Another embodiment of present invention provides a safe, breathable anti-viral and anti-bactefial barrier for preventing micro-organisms to pass through, wherein the complex of lipophilic, anti-oxidant, anti-viral and/or anti-bacterial agent is a tocopherol-curcumin complex.
Another embodiment of present invention provides a safe, breathable anti-viral and anti-bacterial barrier for preventing micro-organisms to pass through, wherein primary lipophilic agent acting as adsorbent is Tocopherol;
Another embodiment of present invention provides a safe, breathable anti-viral and anti-bacterial barrier for preventing micro-organisms to pass through, wherein antioxidant agents are Tocopherol (Vitamin E); and Curcumin.
Another embodiment of present invention provides a safe, breathable anti-viral and anti-bacterial barrier for preventing micro-organisms to pass through, wherein anti¬viral and anti-bacterial agents are Tocopherol and Curcumin.
Another embodiment of present invention provides a safe, breathable anti-viral and anti-bacterial barrier for preventing micro-organisms to pass through, wherein the curcumin for tocopherol-curcumin complex is obtained from curcuminoids mixtures with or without the means of extended shelf life either in native form or encapsulated form.
Another embodiment of present invention provides a safe, breathable anti-viral and anti-bacterial barrier for preventing micro-organisms to pass through, wherein the curcuminoids mixtures contains curcumin, demethoxy curcumin, bis-demethoxy curcumin and/or turmeric oleoresin and/or defatted turmeric oleoresin and/or mixtures thereof.
Another embodiment of present invention provides a safe, breathable anti-viral and anti-bacterial barrier for preventing micro-organisms to pass through, wherein the curcumin content in tocopherol-curcumin complex is 0.001% to 95.0% (w/w).

Another embodiment of present invention provides a safe, breathable anti-viral and anti-bacterial barrier for preventing micro-organisms to pass through, wherein the tocopherol content in tocopherol-curcumin complex is 0.001 % to 95.0% (w/w).
Another embodiment of present invention provides a safe, breathable anti-viral and anti-bacterial barrier for preventing micro-organisms to pass through, wherein the tocopherol for tocopherol-curcumin complex is obtained from alpha-tocopherol and/or beta-tocopherol and/or gamma-tocopherol and/or delta-tocopherol with or without the means of extended shelf life either in native form or in encapsulated form.
Another embodiment of present invention provides a safe, breathable anti-viral and anti-bacterial barrier for preventing micro-organisms to pass through, wherein the solid porous support material is ranging from 0.25% to 10% of total product.
Another embodiment of present invention provides a safe, breathable anti-viral and anti-bacterial barrier for preventing micro-organisms to pass through, wherein the anti-viral and/or antibacterial agent kills the pathogens in 1 sec to 15 minutes of contact of infectious microorganism.
Another embodiment of present invention provides a respirator for preventing infectious diseases, wherein the anti-bacterial and/or anti-viral agent includes such as but not limited to curcumin and/or turmeric extract and/or curcuminoids mixtures, wherein curcuminoids mixtures contains curcumin, demethoxy curcumin, bis-demethoxy curcumin and/or turmeric oleoresin and/or defatted turmeric oleoresin and/or mixtures thereof, wherein it can be used as a source of curcumin for tocopherol-curcumin complex, with or without the means of extended shelf life either in native form or encapsulated form.
Another embodiment of present invention provides a respirator for preventing infectious diseases, wherein curcumin content in tocopherol-curcumin complex is 0.001% to 95.0% (w/w).
Another embodiment of present invention provides a facemask for preventing infectious diseases, wherein alpha-tocopherol and/or beta-tocopherol and/or

gamma-tocopherol and/or delta-tocopherol alone or mixtures thereof can be used as a source of tocopherol-curcumin complex, with or without the means of extended shelf life either in native form or in encapsulated form.
Another embodiment of present invention provides a respirator for preventing infectious diseases, wherein tocopherol content in tocopherol-curcumin complex is 0.001% to 95.0% (w/w).
Another embodiment of present invention provides a facemask for preventing infectious diseases, wherein the tocopherol-curcumin complex is applied on solid porous matrix that can be any natural and/or non-natural matrix that can further act as a barrier.
Another embodiment of present invention provides a facemask for preventing infectious diseases, wherein solid porous matrix onto which the content of tocopherol-curcumin complex on solid support is ranging from 0.001 % to 95% of total product.
Another embodiment of present invention provides a respirator for preventing infectious diseases, wherein tocopherol-curcumin complex kills the pathogens in 1 sec to 15 minutes of contact of infectious micro-organism.
Another embodiment of present invention provides a facemask for preventing infectious diseases, wherein anti-viral and anti-bacterial barrier works on the principle of adsorption followed by killing and inactivation of enveloped viruses and bacteria, and it need not require use of low pore barriers, making it easy, breathable barrier.
Another embodiment of present invention provides a respirator for preventing infectious diseases, wherein deliver breathable affordable facemask works on principles of adsorption of enveloped viruses and bacteria followed by its killing, wherein inventive step is selective adsorption of enveloped viruses and bacteria followed by bringing it in contact to killing compound.
Another embodiment of present invention provides a facemask for preventing infectious diseases, wherein the killing is done by novel tocopherol-curcumin

complex. After adsorption, pathogens are killed and inactivated by a non-toxic, non-metallic, non-carcinogenic killing agent i.e. novel tocopherol-curcumin complex.
Another embodiment of present invention provides a respirator for preventing infectious diseases, wherein killing agent (tocopherol-curcumin complex) being in bulky nature creates the trap that adsorbs and prevents the low sized enveloped viruses/bacteria to penetrate through the mask. After adsorption and contact, the migration happens immediately for the killing and inactivation by tocopherol-curcumin complex. Curcumin is having broad-spectrum antiviral as well as antibacterial activity, which kills and inactivate the infectious micro-organisms very quickly.
Another embodiment of present invention provides a respirator for preventing infectious diseases, wherein mechanism may be carried out by adsorption followed by killing via tocopherol-curcumin complex and when applied on solid porous support must not require use of any low-pore filters, leading to ease of usage of this barrier and breathing as well as non-toxic nature helps for prolonged usage by the wearer.
Another embodiment of present invention provides a facemask for preventing infectious diseases, wherein the device is better, affordable and safe to the professionals and non-professional peoples. The said device act as first line of defence against harmful enveloped virus and bacterial infections. It kills the micro¬organisms/pathogen very quickly and avoid spreading/propagation of attached viruses and bacteria.
Another embodiment of present invention provides a novel breathable barrier (facemask) for preventing infectious diseases, wherein the breathable barrier kills and not allow enveloped virus and bacteria to pass through the device and protects the wearer from fatal effects of those enveloped viruses and bacterial like flu but not limited to other respiratory illness.

Another embodiment of present invention provides a barrier for preventing infectious diseases, wherein the barrier must not require use of low pores for viruses/bacteria filtration. It is more affordable as compare to other low pore respirators like N95/N90 and have superior specifications as this breathable barrier kills enveloped viruses and bacteria in less than 5 minute of contact.
Additionally, it is non-metallic, having no side effects, no toxicity, even after prolonged use of at least 24 hours. This novel product is meant especially for general public use, where the barriers are required for very large population and affordability is a challenge against fatal disease outbreak.
Another embodiment of present invention provides a barrier for preventing infectious diseases, wherein the barrier is non-toxic in nature and has no harmful effects while it in direct contact with user skin. This can be either by inner side or upper side of the barrier. Upper side of the barrier will have added advantages in terms of spreading of the viruses/bacteria once it come in contact hand, or other means. Killing is so fast that it will not able to spread further.
The following examples are given by way of illustration of the present invention and should not be construed to limit the scope of present disclosure.
Example 1
The barrier is prepared using the non-woven fabric of 0.4 μm porosity acting as a solid support and filtering device, where the Tocopherol-Curcumin complex is used as adsorption and killing components incorporated into it. This barrier was used to protect the wearer from the micro-organism. The concentration of mixed Tocopherol in Tocopherol-curcumin complex is 30% w/w, balance 30%w/w is curcumin, 20%w/w is demethoxy curcumin and 20%w/w is bis-demethoxy curcumin. Total content of Tocopherol-curcumin complex with respect to fabric is 2%.
In first experiment, a section of 50 cm2 of this barrier was put into petridish and 1 mL of maturely grown culture of organism E. coli (250 CFU/mL) was poured onto.

These plates were incubated at 37 degC, for 24 hours. As a result it was found that only 2 CFU/mL (i.e. 1 %) of the micro-organism could survive with this treatment.
Example 2
Additionally, 50 cm2 of this barrier as prepared in Example 1, another barrier was 5 prepared using classical N95 Fabric instead of 0.4 u porosity, and used for the studies. Test fabric was kept on a suitable support, and air containing bio-burden (Staphylococcus) of 108 CFU/m3 was passed through this barrier. Empty - sterile petridish was kept behind the barrier in aseptic condition to check any survival after passing through this barrier. The results after passing this air for 3 hours at the 10 velocity of (0.00025m3/sec ~ typical breathing velocity). Anti-microbial activity was compared with other traditional means, i.e. Classical N95 Fabric (Just filtration), Classical N95 fabric + Killing agent (Filtration + Killing) and no barrier was kept as a control. The results are as shown in table 1.
Table 1: Results of anti-microbial activity with the present invention

Type CFU / petri-dish % Reduction
Without any barrier (Control) 255 ±10 NA
Barrier made up of Classical N95 fabric (Filtration) 13 ±3 94.90
Barrier made up of Classical N95 fabric with killing agent (Filtration + Killing) 7±2 97.25
Novel Barrier - Barrier with 0.4 μm fabric -Tocopherol-Curcumin complex coating 2±1 99.21
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Example 3
The barrier is prepared using the non-woven fabric of 0.4 μm porosity acting as a solid support and filtering device, where instead of Tocopherol-Curcumin complex, Tocopherol depleted curcuminoids mixture was incorporated into it. Instead of
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tocopherol non-reactive small size fatty acid was used to prepare the complex. (Thus composition of complex is 0%w/w is Tocopherol, 30%w/w is Curcumin, 20%w/w is demethoxy curcumin and 20%w/w is bis-demethoxy curcumin , and balance 30% is non-reactive small size fatty acid) This Tocopherol depleted barrier was tested for protection of the wearer from the micro-organism. The Total Content of Tocopherol depleted Curcuminoids mixture with respect to fabric was same as example 2 i.e. 2%.
Additionally, 50 cm2 of this barrier as prepared as above was kept on a suitable support, and air containing bio-burden (Staphylococcus) of 108 CFU/m3 was passed through this barrier. Empty - sterile petridish was kept behind the barrier in aseptic condition to check any survival after passing through this barrier. The results after passing this air for 3 hours at the velocity of (0.00025m3/sec ~ typical breathing velocity). Anti-microbial activity was compared with other traditional means, i.e. Classical N95 Fabric (Just filtration), Classical N95 fabric + Killing agent (Filtration + Killing) and no barrier was kept as a control, as done in example 2. The results are as shown in table 2.
Table 2: Results of anti-microbial activity with the present invention (Tocopherol depleted Curcumin Coating)

Type CFU / petri-dish % Reduction
Without any barrier (Control) 255 ±10 NA
0.4 micron fabric - uncoated 18±3 92.94
Barrier made up of Classical N95 fabric (Filtration) 13 ±3 94.90
Barrier made up of Classical N95 fabric with killing agent (Filtration + Killing) 7±2 97.25
Present Study - Barrier with 0.4 urn fabric -Tocopherol depleted - Curcumin Coating 17 ±1 93.33

Example 4
The barrier is prepared using the non-woven fabric of 0.4 um porosity acting as a solid support and filtering device, where instead of Tocopherol-Curcumin complex, 5 curcumin depleted Tocopherol was incorporated into it. Instead of curcumin non-reactive small size fatty acid was used to prepare the complex. (Thus composition of complex is 30 %w/w is Tocopherol, and balance 70% is non-reactive small size fatty acid) This curcumin depleted barrier was tested for protection of the wearer from the micro-organism. The Total Content of curcumin depleted Tocopherol 10 mixture with respect to fabric was same as example 2 i.e. 2%.
Additionally, 50 cm2 of this barrier as prepared as above was kept on a suitable support, and air containing bio-burden (Staphylococcus) of 108 CFU/m3 was passed through this barrier. Empty - sterile petridish was kept behind the barrier in aseptic condition to check any survival after passing through this barrier. The results after 15 passing this air for 3 hours at the velocity of (0.00025m3/sec -typical breathing . velocity). Anti-microbial activity was compared with other traditional means, i.e. Classical N95 Fabric (Just filtration), Classical N95 fabric + Killing agent (Filtration + Killing) and no barrier was kept as a control, as done in example 2. The results are as shown in table 3.
20 Table 3: Results of anti-microbial activity with the present invention (Curcumin depleted Tocopherol Coating)

Type CFU / petri-dish % Reduction
Without any barrier (Control) 255 ±10 NA
0.4 micron fabric - uncoated 18±3 92.94
Barrier made up of Classical N95 fabric (Filtration) 13 ±3 94.90

Type CFU / petri-dish % Reduction
Barrier made up of Classical N95 fabric with killing agent (Filtration + Killing) ■7 ±2 97.25
Present Study - Barrier with 0.4 urn fabric -Curcumin depleted -Tocopherol Coating 14 ±1 94.50
Example 5
The barriers prepared in example 1 (barrier coated with Tocopherol-Curcumin Complex), 3 (barrier coated with Tocopherol depleted Curcumin Complex), and 4 (barrier coated with Curcumin depleted Tocopherol) were tested for the breathability. By passing the air the flow of 8 Lit/min (Method of Bureau of Indian Standards Method - IS 16289) form the barrier sample of 4.9 cm2 (2.5 cm diameter). The differential pressure reported as breathability was measured. The test was compared with the Surgical masks (3 Ply), Barrier made up of Classical N95 fabric (Filtration), Barrier made up of Classical N95 fabric with Killing agent (Filtration + Killing).
Table 4: Results of breathability (Differential pressure) comparison with different coating

Type Differential Pressure (Pa)
0.4 micron fabric - uncoated 20.0 ±0.2
BIS 16289 Class 2, 3 ply Surgical mask 29.4 ±0.3
Barrier made up of Classical N95 fabric (Filtration) 49.0 ±0.5
Barrier made up of Classical N95 fabric with killing agent (Filtration + Killing) 49.0 ±0.3

Present Study - Barrier with 0.4 μm fabric -Tocopherol depleted - Curcumin Coating 20.0 ± 0.2
Present Study - Barrier with 0.4 μm fabric - Curcumin depleted - Tocopherol Coating 28.0 ± 0.3
Novel Barrier - Barrier with 0.4 μm fabric - Tocopherol-Curcumin complex coating 28.2 ±0.3
Example 6
The Tocopherol-Curcumin Complex prepared in example 1, was coated on the fabric in such a way that the final Total content of Tocopherol-curcumin complex with respect to fabric is 10 %. This barrier was tested for breathability and filtration+killing as per protocols mentioned into example 2 and 5.

Type Differential Pressure (Pa) CFU / petri-dish
0.4 micron fabric - uncoated 20.0 ±0.2 255 ±10
Novel Barrier - Barrier with 0.4 urn fabric -Tocopherol-Curcumin complex coating (2% Coating) 28.2 ± 0.3 2±1
Novel Barrier - Barrier with 0.4 μm fabric -Tocopherol-Curcumin complex coating (10% Coating) - This Example 33.4 ±0.3 1±1
Example 7
The barrier is prepared using the non-woven fabric of 0.65 μm porosity acting as a solid support and filtering device, where the Tocopherol-Curcumin complex is used as adsorption and killing components incorporated into it. This barrier was used to protect the wearer from the micro-organism. The concentration of mixed Tocopherol in Tocopherol-curcumin complex is 40% w/w, balance 27%w/w is

curcumin, 18%w/w is demethoxy curcumin and 18%w/w is bis-demethoxy curcumin. Total content of Tocopherol-curcumin complex with respect to fabric is 10% This barrier was tested for breathability and filtration + killing as per protocols mentioned into example 2 and 5.

Type Differential Pressure (Pa) CFU / petri-dish
0.4 micron fabric - uncoated 20.0 ± 0.2 .255 ±10
Novel Barrier - Barrier with 0.4 μm fabric -Tocopherol-Curcumin complex (30% Tocopherol 70% curcumin) coating (2% Coating) 28.2 ±0.3 2±1
Novel Barrier - Barrier with 0.65 μm fabric -Tocopherol-Curcumin complex (40% Tocopherol 60% curcumin) coating (10% Coating) - This Example 25.2 ± 0.3 3±1
Example 8
The barrier is prepared using the non-woven fabric of 0.4 um porosity acting as a solid support and filtering device, where the Tocopherol-Curcumin complex is used as adsorption and killing components incorporated into it. This barrier was used to protect the wearer from the micro-organism. The concentration of mixed Tocopherol in Tocopherol-curcumin complex is 10% w/w, balance 37%w/w is curcumin, 27%w/w is demethoxy curcumin and 26%w/w is bis-demethoxy curcumin. Total content of Tocopherol-curcumin complex with respect to fabric is 2%. This barrier was tested for breathability and filtration + killing as per protocols mentioned into example 2 and 5.

Type Differential Pressure (Pa) CFU / petri-dish
0.4 micron fabric - uncoated 20.0 ± 0.2 255 ±10

Novel Barrier - Barrier with 0.4 μm fabric -Tocopherol-Curcumin complex (30% Tocopherol 70% curcumin) coating (2% Coating) 28.2 ±0.3 2±1
Novel Barrier - Barrier with 0.4 μm fabric -Tocopherol-Curcumin complex (10% Tocopherol 90% curcumin) coating (2% Coating) - This Example 22,2 ±0.3 4±1
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We Claim:
1. A safe, breathable anti-viral and/or anti-bacterial barrier for preventing micro-
organisms to pass through, the barrier comprising:
a) a solid breathable porous support material/s; and
b) a complex of lipophilic, anti-oxidant, anti-bacterial and anti-viral agent(s) which is responsible for adsorption of micro-organisms and bringing it in next to inactivating/killing agent present in the complex.
2. The barrier as claimed in claim 1, wherein the solid breathable porous support
material is any natural and/or non-natural breathable porous matrix.
,3. The barrier as claimed in claim 1, wherein the solid breathable porous support material is having pore size in the range of 0.4 um to 0.7 um.
4. The barrier as claimed in claim 1, wherein the complex of lipophilic, anti-oxidant, anti-viral and anti-bacterial agent is a tocopherol-curcumin complex.
5. The barrier as claimed in claim 1, wherein the lipophilic agent is Tocopherol.
6. The barrier as claimed in claim 1, wherein the anti-oxidant is Tocopherol (Vitamin E) and Curcumin.
7. The barrier as claimed in claim 1, wherein the anti-viral and anti-bacterial agent is Tocopherol.
8. The barrier as claimed in claim 1, wherein the anti-viral and anti-bacterial agent is Curcumin.
9. The barrier as claimed in claim 8, wherein the curcumin for tocopherol-eurcumin complex is obtained from curcuminoids mixtures with or without the means of extended shelf life either in native form or encapsulated form.
10. The barrier as claimed in claim 9, wherein the curcuminoids mixtures contains curcumin, demethoxy curcumin, bis-demethoxy curcumin and/or turmeric oleoresin and/or defatted turmeric oleoresin and/or mixtures thereof.
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11. The barrier as claimed in claim 7, wherein the tocopherol for tocopherol-curcumin complex is obtained from alpha-tocopherol and/or beta-tocopherol and/or gamma-tocopherol and/or delta-tocopherol with or without the means of extended shelf life either in native form or in encapsulated form.
12. The barrier as claimed in claim 1 and 4, wherein the tocopherol-curcumin complex is applied on solid breathable porous support material(s).
13. The barrier as claimed in claim 1 and 4, wherein the Tocopherol-Curcumin complex is ranging from 0.5% to 10% of total product.
14. The barrier as claimed in claim 1, wherein the anti-viral and/or antibacterial agent kills the pathogens in 1 sec to 15 minutes of contact of infectious micro-organism.
15. The barrier as claimed in claim 1, wherein ratio of tocopherol to curcumin in the complex is 40:60, 30:70, 20:80, 10:90.