Claims:1. A screening method for the identification of molecules which promote hair growth, said method comprising:
a. obtaining at least one molecule; and
b. carrying out in silico docking simulation studies between said molecule and at least one polypeptide selected from the group consisting of ADP ribosylation factor like protein 1, transient receptor potential cation channel subfamily V member 2, synaptobrevin YKT6, and combinations thereof,
wherein docking of said molecule with said polypeptide is indicative of said molecule being able to promote hair growth.
2. A screening method for the identification of molecules which promote hair growth, said method comprising:
a. obtaining at least one molecule; and
b. contacting said molecule with at least one polypeptide selected from the group consisting of ADP ribosylation factor like protein 1, transient receptor potential cation channel subfamily V member 2, synaptobrevin YKT6, and combinations thereof,
wherein binding of said molecule with said polypeptide is indicative of said molecule being able to promote hair growth.
3. A screening method for the identification of molecules which promote hair growth, said method comprising:
a. obtaining at least one molecule;
b. carrying out in silico docking simulation studies between said molecule and at least one polypeptide selected from the group consisting of ADP ribosylation factor like protein 1, transient receptor potential cation channel subfamily V member 2, synaptobrevin YKT6, and combinations thereof;
c. identifying polypeptide(s) that bind to said molecule; and
d. obtaining polypeptide(s) as identified in step (c), and contacting said molecule with said polypeptide,
wherein binding of said molecule with said polypeptide is indicative of said molecule being able to promote hair growth.
4. The method as claimed in claims 1-3, wherein said molecule can be a biological molecule, a chemical molecule, or a complex comprising biological or chemical molecules.
5. The method as claimed in claims 2-3, wherein said polypeptide is purified polypeptide or present in dermal papillae cell lysate.
6. The method as claimed in claims 2-3, wherein said contacting of molecule with polypeptide is carried out by immobilizing said molecule on a polymer matrix.
7. The method as claimed in claim 6, wherein said polymer matrix is able to retain at least 40% of said immobilized molecule upon repeated washing.
8. The method as claimed in claims 1-3, wherein said polypeptide is ADP ribosylation factor like protein 1.
9. The method as claimed in claims 1-3, wherein said polypeptide is a combination of ADP ribosylation factor like protein 1, and transient receptor potential cation channel subfamily V member 2.
The method as claimed in claims 1, and 3, wherein said molecule docks with ADP ribosylation factor like protein 1 in a particular configuration at least 10% of the time, or with transient receptor potential cation channel subfamily V member 2 at least 10% of the time.
10. The method as claimed in any of the claims 1-3, wherein said molecule is climbazole.
, Description:As Attached