Form 2
Patents Act, 1970,
(39 of 1970)
&
The Patent rule 2003,
Complete Specification
"seaweed liquid fertilizers"
Dr. Annasaheb S. Khemnar,
Indian National
Director, The Institute of Science, Madam cama road, Mumbai 32;
The following specification particularly describes the invention and manner in which it is to be performed;

The field of invention;
The field of invention relates to liquid fertilizers, more particularly the field of invention relates to seaweed liquid fertilizers; Background of the invention and prior art;
Seaweed refers to several species of macroscopic, multicellular, marine algae. The term includes some types of red, brown and green algae.
Seaweed belongs to one of several groups of multicellular algae: the red algae, green algae, and brown algae. As these three groups do not have a common multicellular ancestor, the seaweed is in a polyphyletic group.
Seaweed has been used in milled or finely divided form and in the form of a liquid extract. Seaweed extracts have been shown to increase crop yields, resistance of plants to frost, uptake of inorganic soil nutrients, resistance to environmental stress conditions, resistance to some pests such as red spider mite and aphids and to reduce fruit losses during storage.
Application of seaweed extracts improve the root system of plants and increase their reproductive capacity, increasing the number of flowers per plant as well as the number of flowers per flowering season.
Seaweed species are grouped into red, green and brown seaweeds. The species used to produce extracts varies throughout the world. Most commercially available seaweed fertilisers, also called bio-stimulants, are made from brown seaweeds. Brown seaweeds are more prevalent in cold water regions of the world.
With regard to plant nutrition concerns it is essential that adequate nitrogen in the contents of soil is necessary for a healthy lawn, plants, and crops. Fertilizers containing nitrogen are used to enhance the nitrogen levels in the soil to produce greener, lusher, faster-growing plants, lawns, and crops. Nitrogen must be available for the plant to use it, meaning that the nitrogen must be converted to NH4+ or NH3. The Nitrogen may be readily available or organisms in the soil must covert the Nitrogen into a usable form. From the prior art documents it is revealed that seaweed is extensively exploited as fertilizers, for instance,

Indian Patent application number: 201621006923 teachhighly Soluble N-P-K Fertilizer with Seaweed extract and Spirulina for use in Agriculture. A water soluble N-P-K fertilizer composition that combines the benefits of Seaweed extract containing naturally occurring trace and microelements and Spirulina containing amino acids with a neutral pH and free from chlorides is prepared. The inventive product is highly water soluble and therefore offers the benefit of easy absorption as compared to conventional water soluble fertilizers.
Another * Indian Patent application number 5794/CHE/2014 discloses pesticides and specifically to botanical formulation for crop pest and phytopathogens management comprising one or more emulsifiable concentrations of seaweed extracts. Particularly, the present invention relates to non-toxic, biodegradable and environmentally friendly seaweed botanical formulation that reduces pest infestation along with antimicrobial capabilities.
Indian Patent application Number: 3711/DEL/2013 discloses antibacterial property of green Seaweed Chaetomorpha sp. against Helicobacter pylori f 9 ~f C. The present invention relates to the use of bioactive extract isolated from the algae as potent anti- Helicobacter pylori agent.
Indian application number 4133/CHE/2013 a seaweed farming system (100) for farming of seaweed in a water body is described herein. In an embodiment, the seaweed farming system (100) includes a seaweed farm operating assembly (102) which further includes a harvesting unit (106) to separate vegetative portion of seaweed from mature seaweed.
Indian application number 183/CAL/1974 teaches a process for the preparation of agar from Indian Red Seaweed, namely Felidiells mcerces collected from Deoki, Dhamlej region of Veraval coast in Saurashtra which consists in i) pretreatment such as shredding, soaking and wet grinding of the seaweed, ii) prospers extraction of the pulp at 10 p.s.i.c for 2 hours in an autoclave after adjusting the pH to 5.8 - 6.0 with 1 H sulphuric acid, iii) filtration of the hot extract using diatomaceous earth in a pressure filter, iv) freezing-thawing of agar gel, and purification of the crude agar by re-dissolving in distilled water and repeated freeze-thawing.
Moreover, it is known that Inorganic fertilizers, while inexpensive and easy to apply, tend to wash the nutrients out of the soil, requiring reapplication on a regular basis. Additionally,

since inorganic chemical fertilizers are concentrated, they tend to burn plant roots more than organic materials.
Therefore, in the present invention it is provided that local seaweed is used for making the fertilizer composition in the water miscible solvent or in aqueous base;
Object of the invention;
It is an object of the invention to provide a method for preparation of an extract of seaweeds for preparation of liquid fertilizers;
It is also an object of the invention to provide combination of an effective amount of extract of seaweed for preparation of a liquid fertilizer;
It is also an object of the invention to provide optimum concentration exhibiting maximum potential as seaweed liquid fertilizer.
It is also an object of the invention to provide suitable carriers/ solvents or combination of solvents for extraction of seaweeds.
It is also an object of the invention to assess of selected seaweeds individually and in mixture as seaweed liquid fertilizers.
Most importantly, it is an object of the invention to provide a liquid organic fertilizers which overcomes the ill effect of the chemical fertilizers which is also an efficient and cost effective;
It is also an object of the present invention to provide a environmentally friendly fertilizer;
It is also an object of the invention to provide a comparative testing of seaweed liquid fertilizer developed for its potency with commonly used commercial seaweed liquid fertilizers in Maharashtra.
Summary of the invention;
In one of the important aspect of the invention it is provided that a Seaweed extract in a solvent or in combination of solvent is extracted and effective amount of the extract an excess solvent is used as liquid fertilizer, the Seaweed includes from various families induces Chlorophyceae, Rhodophyceae and Phaeophyceae families or combination of thereof;

The seaweed of the present invention seaweeds selected for liquid fertilizer composition is Ulva fasciata Delite, Sargassum tennerimum J. Ag. and Hypnea valentiae (Turn) Mont or mixture thereof, the mixture of seaweed liquid extract in a solvent is collectively named as 'Sagarsanjivani';
Preparation of composition of the seaweed extract as 'Sagarsanjivani';
The aqueous extract prepared from the sea weeds Ulva fasciata Delite, Sargassum tennerimum J. Ag. and Hypnea valentiae (Turn) Mont in the concentration of 100% mixed in the ratio of 1:1:1 v/v to obtain composition of seaweed extract which is then compared as fertilizer with branded known variant to obtain morphological and biochemical results which as depicted in table 2-4;
In the other aspect of the invention a method for preparation of an extract of the seaweed is also provided after drying and extraction in solvent includes water or water miscible alcohol, further providing concentration in an effective amount;
Detailed Description of invention;
In one of the important aspect of the invention provides method for preparation of extract in a solvent for which of seaweeds were collected from Malvan, Sindhudurg-fort and Kunkeshwar, of Sindhudurg district, Burondi and Harihareshwar of Raigad district and Colaba from Mumbai along the coast of Maharashtra.
Fourteen common and abundant seaweeds among sixty belonging to chlorophyceae, rhodophyceae and phaeophyceae were identified for screening their potential as seaweed liquid fertilizer. The collections of these seaweeds were made during low tides from the said locations. The seaweeds collected were washed thoroughly first with seawater and then with fresh water to remove sand particles and epiphytes. These were shade dried for four days, followed by oven drying at 60°C and was used for preparation of extracts used; In one of the most important aspect of the invention the seaweed is selected from the following table 1, which includes seaweeds from different groups green seaweeds, brown seaweeds, red seaweeds;

Table 1.
Sr. No. Name of algae Place of collection
Green seaweeds
1. Ulva faciata Delile. Harihareshwar
2. Ulva faciata Delile. Harihareshwar
3. Enteromorpha compressa L. Harihareshwar
4. Enteromorpha tubulosa Kuetz. Burondi
5. Cauler paracemosa (Forsk) Weber Sindhudurg fort
Brown seaweeds
6. Padina trastomatica Hauck. Colaba (Mumbai)
7. Dictyota dichotoma (Huds.) Lamour Colaba (Mumbai)
8. Laurencia intermedia Yamada Colaba (Mumbai)
9. Sargassum cinerum J. Ag. Sindhudurg fort
10. Sargassum tennerimum J.Ag. Malvan
Redseaweed Hypnea valentiae (Turn) Mont. Kunkeshwar
Ii: Hypnea musciformis (Gulf.) Lamour Colaba (Mumbai)
13. Garacillaria corticata J. Ag. Kunkeshwar
14. Spathoglossum asperum J. Ag. Malvan
In an another aspect of the invention a method for preparation of liquid extract of seaweed is provided as, whereas the liquid for extraction includes water, and or water soluble alcohols; The method for extraction is as described below, Aqueous seaweed liquid extract:
Seaweeds are collected from places listed in table-A were washed with seawater to remove epiphytes and other sedimentary particles and ash, These seaweeds were washed repeatedly with tap water to remove salts, then dried in the shade and kept finally in the oven at 60 ± 5°C temperatures for 24 hrs to ensure complete removal of moisture. The fully dried material was used for preparation of liquid extracts.

Cold aqueous seaweed liquid extract:
The fully-dried seaweed materials were chopped/powdered and used for preparation of cold extract by immersing them in demineralised water for an hour in 1:5 proportion(One part seaweed: Five part water). Thereafter, it was filtered through muslin cloth under pressure and centrifuged at 5000 rpm for 15-20 minutes to remove sedimentary particles. The supernatant, thus obtained, was designated as cold extract.
Boiled aqueous seaweed liquid extract:
In the preparation of boiled extract, the chopped seaweeds were boiled with demineralised
water in the ratio of 1:5 for an hour at 100°C. After an hour, the mixture was squeezed under
pressure and filtered through muslin cloth and centrifuged at 5000 rpm. The supernatant was
used in preparation of various dilutions of boiled aqueous extract.
The volume of filtrate in both cold and boiled extracts was adjusted to the corresponding
value in terms of weight of seaweeds. This was arrived at either by evaporation in the case of
excess volume or by further dilution in at the ratio of 100 gm.
Seaweed: 100 ml final volume of extract or 500 gm of seaweed: 500 ml (final volume of
extract).
The supernatant, thus obtained, after centrifugation in both cold and boiled extracts was
considered as 100 % seaweed extract. From this extract different concentrations were
prepared with demineralised water for their use as seaweed liquid extract. The various
concentrations tried were 5, 10, 15, 20, 25, 50, 75 & 100%.
Preparation of alcoholic extract:
The alcoholic extracts were prepared by boiling each seaweed sample separately for an hour
with 80% alcohol in the ratio of 1:5 (one part seaweed and five parts alcohol).
The alcoholic mixture, was then, filtered through muslin cloth. Each filtrate, thus obtained,
was condensed to 2-3 ml by boiling it on water bath at 100° C. The final volume of the filtrate
was adjusted to the weight of the material taken, using demineralised water. The filtrate was
considered to be 100% alcoholic seaweed liquid extract. Different concentrations (5, 10, 15,
20 & 25%) were prepared from this using demineralised water for experimental purpose. All
the extracts were stored in the refrigerator;

In an another aspect of the invention seaweeds selected for liquid fertilizer composition is Ulva fasciata Delite, Sargassum tennerimum J. Ag., and Hypnea valentiae (Turn) Mont or mixture thereof, the mixture of seaweed liquid extract in a solvent 4s collectively named as 'Sagarsanjivani';
Further, the effectiveness of the 'Sagarsanjivani' is compared with commercially available liquid fertilizer composition having brand name as 'Biozyme';
Experiments were also conducted to determine the relationship between the nutrient status of test plant (as influenced by aforesaid treatments) and the vegetative growth, accumulation of biomass and biochemical constituents etc. High enhancement in elements like N, P, K, Ca, S, Na, Fe, Zn and Cu has been attributed to the treatment of 'Sagarsanjivani' more than to the treatment with the commercial seaweed liquid-fertilizer 'Biozyme'.
However, a great enhancement in Mg content has been noticed in plants treated with individual SLE of U. fasciata Delite, S. tennerimum J. Ag., as well as 'Sagarsanjivani' that of plants treated with 'Biozyme'.
Among the 14 seaweeds Ulva fasciata Delite, Sargassum tennerimum J. Ag. and Hypnea
valentiae (Turn) Mont exhibited better potential as raw material for production of the
seaweed liquid fertilizers.
Intensive studies indicated that, the composite mixture of aforesaid three seaweeds liquid
extracts collectively called as 'Sagarsanjivani' yielded better results in terms of all the
parameters as compared to their individual applications carried out on the vegetable plant
fenugreek (Trigonella foenum graecum L., of Family Leguminoceae) which is commonly
known as Methi, is cultivated throughout India for culinary and medicinal purposes as well as
for fodder.
Application of seaweed liquid extract at seed germination in fenugreek;
Preparation of germination bed:
The certified seeds were procured from the Seed Testing Department, Government of
Maharashtra, Krishibhavan, Pune. The seeds were sterilised with 0.1% mercuric chloride for

one or two minutes, then rinsed repeatedly with sterilised distilled water. The control set was maintained by using distilled water.
Application of seaweed extracts:
In the first phase of preliminary experiments, concentrations of 10,25, 50, 75 & 100 % of Seaweed liquid extract (SLE) were used. However, in the second phase of the preliminary experiments, the concentrations of 5,10,15,20 & 25 % were applied as better results were obtained in the treatments at lower concentrations of SLE.
However, culture studies the seaweed liquid extracts were used in the form of foliar application. Foliar sprays were given 10 days after emergence of seedlings. Two more applications were given at an interval of 10 days. Both the phases of the experiments were undertaken in triplicates.
Measurement of growth of seedling:
The percentage of germination for the first five days was recorded. The seedling-growth in terms of length of main root, shoot, total height and number of lateral roots were measured after a period of days. The data were also used to determine the ratio of root to shoot.
Measurement of fresh and dry weight:
After a period of ten days the seedlings from each treatment were weighed using Anamade balance to record their fresh weight. They were, then kept in an oven at 60± 5oC for 48 hours. They were weighed again to record their dry weights.
Biochemical analysis includes, Estimation of chlorophylls , carbohydrates, Reducing and non-reducing sugars, Estimation of starch, Estimation of proteins, , Estimation of total phenols, Ascorbic acid, , Extraction and assay of enzyme Peroxidase ,Extraction and assay for Polyphenol oxidase
Elemental analysis of seaweeds used and test plant, Triple acid digestion for P, K, Ca, Mg, S, and micronutrients:

Biochemical analysis includes, Estimation of chlorophylls, carbohydrates, Reducing and non-reducing sugars, Estimation of starch, Estimation of proteins, Estimation of total phenols, Ascorbic acid, Extraction and assay of enzyme Peroxidase, Extraction and assay for Polyphenol oxidase.
Elemental analysis of seaweeds used and test plant, triple acid digestion for P, K, Ca, Mg, S, and micronutrients:
Analyses of data generated through the experiments using 'Sagarsanjivani' and commercially available seaweed liquid fertilizer 'Biozyme crop+' clearly indicated, the positive results of 'Sagarsanjivani' with reference to the morpho-physiological growth functions as well as the biochemical and elemental constituents.
'Sagarsanjivani' contains all the macronutrients and necessary trace elements required for improving the overall health of plants. Moreover, it, presumably, contains the growth regulators, enzymes and biostimulants, which would certainly be beneficial in increasing the productivity-status of crop plants. These compounds have been suggested as the active ingredients, which are partially responsible for the improvement in growth observed with respect to seaweed products. Moreover, many of the beneficial effects of the seaweed treatments on plants, resemble those obtained following the application of plant regulators.
Table 2; Effect of S. tennerimum J. Ag., U. fasciata Delite, H. valentiae (Turn) Mont individual SLE, Sagarsanjivani, and Biozyme on the growth rate of Fenugreek.

Treatments Absolute Growth Rate (AGR) Mean Relative Growth Rate (RGR) Mean Leaf area Duration (LAD)
Control 0.072 0.03925 7.92
S .tennerimum J. Ag. 0.087 0.03446 10.78
U. fasciata Delite 0.073 0003134 12.98
H.valentiae (Turn) Mont 0.105 0.04217 22.98
Sagarsanjivani 0.119 0.03743 15.80
Biozyme (RD)* 0.089 0.03563 12.18

Table 3: Effect of S. tennerimum J. Ag., U. fasciata Delite, H. valentiae (Turn) Mont individual LEs, Sagarsanjivani, and Biozyme onthedrymatter of Root, Stemand Leaf (gm.) per 5 plants in fenugreek.

Treatment Mean dry matter of roots Mean dry matter of stem Mean dry matter of leaves Mean total dry matter
(After35Days)
Control 0.03 0.15 0.30 0.48
S. tennerimum J. Ag. 0.04 0.23 0.44 0.71
U. fasciata Delite 0.04 0.21 0.44 0.69
H. valentiae (Turn) Mont 0.04 0.19 0.42 0.65
Sagarsanjivani 0.06 0.28 0.53 0.87
Biozyme* (RD) 0.04 0.20 0.46 0.70
S. E. 0.00578 0.01048 0.01176 0.01746
C. D. at5% 0.01685 0.03061 0.03433 0.05097
(After 35
Days) control 0.08 0.53 0.60 1.21
S. tennerimum J. Ag. 0.12 0.61 0.84 1.57
U. fasciata Delite 0.11 0.57 0.74 1.42
H. valentiae (Turn) Mont 0.12 0.70 0.87 1.69
Sagarsanjivani 0.14 0.94 0.98 2.06
Biozyme*(RD) 0.1 0.69 0.79 1.59
S.E. 0.00796 0.00775 0.01033 0.01638
C. D. at 5% 0.02322 0.02261 0.03014 0.04781

Table 4: Effect of S. tennerimum J. Ag., U. fasciata Delite, H. valentiae (T Biozyme on biochemical constituents in Fenugreek. urn) Mont individual SLEs, Sagarsanjivani and
Parameters Control U. fasciata Delite S. tennerimum
J. Ag. H. valentiae
(Turn) Mont Sagarsanjivani Biozyme (RD)* S.E. C.D. at 5%
Chlorophyll-a mg/gm 0.3840 0.4399 0.3887 0.4309 0.4578 0.3923 0.00013 0.00041
Chlorophyll-b mg/gm 0.5939 0.5969 0.6158 0.6542 0.6489 0.618 0.00014 0.00044
Total chlorophylls mg/gm 0.977 1.020 1.004 1.084 1.106 0.996 0.00012 0.00370
Proteins mg/gm 09.50 10.83 13.39 15.29 17.50 12.00 0.01478 0.04552
Polyphenols mg/gm 1.316 1.873 1.950 3.281 4.875 2.340 0.00129 0.00394
Non reducing sugar mg/gm 14.472 19.028 15.378 16.492 21.492 14.190 0.00390 0.01203
Reducing sugar mg/gm 0.798 0.931 0.972 1.057 1.180 0.896 0.00137 0.00421
Total sugar mg/gm 15.270 19.959 16.350 17.549 22.672 15.086 0.00490 0.01514
Starch mg/gm 1.716 2.224 2.420 2.244 3.300 1.980 0.00175 0.00536
Ascorbic acid mg/100gm 137.08 220.48 231.90 239.99 259.31 212.48 0.02329 0.07177
*RD=Recomm endedDose

Table 5: Elemental composition in U. fasciata Delite, S. tennerimum J. Ag. and H. valentiae (Turn) Mont
Name of Element U. fasciata S. tennerimum J. H. valentiae S.E. C.D.at5%
Delite Ag. (Turn) Mont
Total Nitrogen% 3.8 5.4 4.3 0.0577 0.1998
Total Phosphorus% 0.2 0.3 0.2 0.0120 0.0416
Total Potassium% 1.1 1.9 0.7 0.1459 0.5049
Calcium% 1.90 4.93 5.60 0.0176 0.0609
Magnesium% 2.16 0.73 0.67 0.0120 0.0415
Sulphur% 2.33 1.03 1.91 0.0091 0.0314
Iron % 0.2 0.4 1.0 0.0100 0.0346
Manganese (ppm) 80 200 320 1.0001 3.4605
Zinc (ppm) 30 14 28 0.5774 1.9978
Copper (ppm) 30 30 20 0.8166 2.8254
Boron (ppm) 13 91 198 1.2020 4.1589

Table 6: Effect of U. fasciata Delite, S. tennerimum J. Ag., H. valentiae (Turn) Mont individual SLE, Sagarsanjiv Biozyme on
Elemental composition in Fenugreek. ani and
Element Control U. fasciata
Delite S. tennerimun
J.Ag. H.valentiae
(Turn) Mont Sagarsanjivani Biozyme (RD)* S.E. CD. at 5%
Total Nitrogen % 2.52 4.20 3.64 3.64 4.20 3.08 0.0076 0.0234
Total Phosphorus
% 0.43 0.43 0.48 0.40 0.48 0.45 0.0094 0.0291
Total Potassium % 3.00 3.05 3.40 3.00 3.60 3.00 0.0127 0.0392
Calcium % 2.47 2.48 2.39 2.60 2.60 2.64 0.0108 0.0332
Magnesium % 0.50 0.58 0.52 0.48 0.56 0.51 0.0094 0.0291
Sulphur % 0.34 0.42 0.35 0.37 0.44 0.40 0.0094 0.0290
Sodium % 0.30 0.31 0.30 0.32 0.31 0.25 0.0071 0.0217
Iron (ppm) 271 261 273 272 326 217 1.0001 3.0812
Manganese (ppm) 34 41 44 41 54 40 0.8166 2.5158
Zinc (ppm) 32 36 36 31 38 32 0.5774 1.7789
Copper (ppm) 14 19 15 13 25 13 0.5846 1.8011
*RD= Recommended Dose

Examples;
Aqueous seaweed liquid extract:
100 grams of Seaweed, Ulva fasciata Delite, was washed with seawater to remove epiphytes and other sedimentary particles and wash, These seaweeds were washed repeatedly with tap water to remove salts, then dried in the shade and kept finally in the oven at 60 ± 5°C temperatures for 24 hrs to ensure complete removal of moisture. The fully dried material was used for preparation of liquid extracts.
Cold aqueous seaweed liquid extract:
The fully-dried seaweed Ulva fasciata Delite, were chopped/powdered and used for preparation of cold extract by immersing them in demineralised water for an hour in 1:5 proportion (One part seaweed: Five part water). Filtered through muslin cloth under pressure and centrifuged at 5000 rpm for 15-20 minutes to remove sedimentary particles. The supernatant, thus obtained, was designated as cold extract.
Boiled aqueous seaweed liquid extract:
In the preparation of boiled extract, the chopped Ulva fasciata Delite were boiled with demineralised water in the ratio of 1:5 for an hour at 100°C. After an hour, the mixture was squeezed under pressure and filtered through muslin cloth and centrifuged at 5000 rpm. The supernatant was used in preparation of various dilutions of boiled aqueous extract.
The volume of filtrate in both cold and boiled extracts was adjusted to the corresponding value in terms of weight of seaweeds. This was arrived at either by evaporation in the case of excess volume or by further dilution in at the ratio of 100 gms.
Seaweed: 100 ml. final volume, of extract or 500 gms of seaweed: 500 ml. (final volume of extract).
The supernatant, thus obtained, after centrifugation in both cold and boiled extracts was used as 100 % seaweed extract. From this extract different concentrations were prepared with demineralised water for their use as seaweed liquid extract. The various concentrations tried were 5, 10, 15, 20, 25, 50, 75 &100%.
Preparation of alcoholic extract:
The alcoholic extracts were prepared by seaweed Ulva fasciata Delite boiling for an hour with 80% alcohol in the ratio of 1:5 (one part seaweed and five parts alcohol).
The alcoholic mixture, was then, filtered through muslin cloth. Each filtrate, thus obtained, was condensed to 2-3 ml by boiling it on water bath at 100 ° C. The final volume of the filtrate was

adjusted to the weight of the sea weed taken, using demineralised water. The filtrate was considered to be 100% alcoholic seaweed liquid extract. Different concentrations (5, 10, 15, 20 & 25%) were prepared from this using demineralised water for experimental purpose. All the extracts were stored in the refrigerator;
Similar procedure repeated for preparation of an extract of the other seaweeds including Sargassum tennerimum J. Ag., Hypnea valentiae (Turn) Mont;
In an another aspect of the invention seaweeds selected for liquid fertilizer composition is Ulva fasciata Delite, Sargassum tennerimum J. Ag., and Hypnea valentiae (Turn) Mont or mixture thereof, the mixture of seaweed liquid extract in a solvent is collectively called as 'Sagarsanjivani';
Preparation of composition of the seaweed extract as 'Sagarsanjivani';
The aqueous extract prepared from the sea weeds Ulva fasciata Delite, Sargassum tennerimum J. Ag., and Hypnea valentiae (Turn) Mont in the concentration of 100% mixed in the ratio of 1:1:1 v/v to obtain composition of seaweed extract which is then compared as fertilizer with branded known variant to obtain morphological and biochemical results which as depicted in table 2-4;
Claims
1. Seaweed liquid fertilizer comprising an effective amount of an extract from seaweed in a solvent or mixture of solvent wherein seaweed is selected from Chlorophyceae, Rhodophyceae and Phaeophyceae families or combination of thereof;
2. Seaweed liquid fertilizer according to claim 1 wherein seaweed is green seaweed includes Ulva faciata Delile. Ulva faciata Defile, Enteromorpha compressa L., Enteromorpha tubulosa Kuetz., Caulerparacemosa (Forsk), brown seaweed includes Padina tetrastomatica Hauck, Dictyotadichotoma (Huds.) Lamour, Laurencia intermedia Yamada, Sargassum tennerium J. Ag., Sargassum tennerimum J. Ag, Hypnea valentiae (Turn) Mont, Hypnea musciformis (Gulf.) Lamou, Garacillaria corticata J.Ag, Spathoglossuma sperum J. Ag. or combination there of;
Seaweed liquid fertilizer according to claim 1 or 2 seaweed is selected from Ulva fasciata Delite, Sargassum tennerimum J. Ag., and Hypnea valentiae (Turn) Mont, or combination thereof; Seaweed liquid fertilizer according to any of the preceding claim wherein solvent is water or water soluble alcohol;

Seaweed liquid fertilizer according to any of the preceding claim an effective amount of an extract of seaweed is 5, 10, 15, 20,25,50,75 & 100%.
5. Seaweed liquid fertilizer according to any of the preceding claim an extract of seaweed Ulva fasciata Delite, Sargassum tennerimum J. Ag. and Hypnea valentiae or combination is in the ratio of 1:1:1 v/v
7. A method for preparation of Seaweed liquid fertilizer comprising
(i) providing cleaned and dried seaweed for making an extract;
(ii) extraction of dried and chopped or powdered Seaweed in a solvent or combination of solvent;
wherein extraction is (iii) cold aqueous seaweed liquid extract:
(iv) boiled aqueous seaweed liquid extract:
(v) mixing of cold and boiled extract in an effective amount to form liquid fertilizer;
8. A method for preparation of Seaweed liquid fertilizer according to claim 7 wherein drying of
Seaweed is 60 to 65 ° C for 24 hrs;
9. A method for preparation of Seaweed liquid fertilizer according to claim 7 wherein of cold
extract is prepared in water for an hour in 1:5 proportion (One part seaweed: Five part water)
further filtered through muslin cloth under pressure and centrifuged at 5000 rpm for 15-20
minutes;
10. A method for preparation of Seaweed liquid fertilizer according to claim 7 wherein boiled
extract is prepared in water in the ratio of 1:5 for an hour at 100°C. for an hour further mixture
was squeezed under pressure and filtered through muslin cloth and centrifuged at 5000 rpm.