Claims:We claim:

1. A seed treatment composition comprising :

a) Pyraclostrobin in an amount ranging from 5-25% w/w
b) Thiamethoxam in an amount ranging from 15-30% w/w
c) Soy protein in an amount ranging from 0.1 to 10% w/w of the composition.
d) At least one agriculturally acceptable excipient

2. The seed treatment composition as claimed in claim 1, wherein said at least one agriculturally acceptable excipient is selected from the group comprising an anti-freeze agent, a dispersing agent, a deformer or anti-foaming agent, a thickener, antibacterial agent, and a dye.
3. The seed treatment composition as claimed in claim 2, wherein said anti-freeze agent may be selected from the group comprising ethylene glycol, propylene glycol or glycerol.
4. The seed treatment composition as claimed in claim 2, wherein said anti-freeze agent is present in an amount ranging from 5-8% w/w of the composition.
5. The seed treatment composition as claimed in claim 2, wherein said dispersing agent may be selected from the group comprising sodium lignosulphonates, sodium naphthalene sulphonate- formaldehyde condensates, aliphatic alcohol ethoxylates, tristyrylphenol ethoxylates and esters, ethylene oxide/propylene oxide block copolymers
6. The seed treatment composition as claimed in claim 2, wherein said dispersing agent is present in an amount ranging from 3-6% w/w of the composition.
7. The seed treatment composition as claimed in claim 2, wherein said deformer or anti-foaming agent may be silicon based.
8. The seed treatment composition as claimed in claim 2, wherein said deformer or antifoaming agent is present in an amount ranging from 0.1 to 0.5% w/w of the composition.
9. The seed treatment composition as claimed in claim 2, wherein said thickener may be selected from the group comprising alginin, guar gum, locust bean gum, and xanthan gum.
10. The seed treatment composition as claimed in claim 2, wherein said thickener is present in an amount ranging from 0.1 to 0.3% w/w/of the composition.
11. The seed treatment composition as claimed in claim 2, wherein said dye is a pigmented dye present in an amount ranging from 2 to 5% w/w of the composition.
12. The seed treatment composition as claimed in claim 1, wherein said composition is formulated in a form selected from the group comprising suspension concentrates, highly concentrated aqueous suspensions, wet dispersible powders, water dispersible granules, flowable concentrates , dispersions and aqueous solutions.
13. The seed treatment composition as claimed in claim 1, wherein said composition is formulated as a Flowable concentrate (FS).
14. The seed treatment composition as claimed in preceding claims, wherein said composition is effective in controlling soil-borne fungal and insect pests.
15. A seed treatment method wherein said method comprises applying the claimed composition to the seeds in an agriculturally effective amount.

Description:FIELD OF INVENTION:
The present invention relates to a novel pesticidal combination for the control of plant pests. More particularly, the present invention relates to novel, synergistic seed treatment composition comprising said pesticidal combination for effective control of insect and fungal pests in various agricultural crops.
BACKGROUND OF THE INVENTION
Combination of insecticides and fungicides are applied to broaden the spectrum of control of insect and fungal pests, reduce dosage, thereby reducing environmental impact, and decrease chances of development of resistance. The combination of insecticides and fungicides at times demonstrate an additive or synergistic effect that results in an improved control on the pests.
Damage to seeds from insect and fungal pests is a major concern for agriculturalists. There are various fungal diseases and insect pests which reduce germination rates and cause considerable decrease in the crop yield. Treating the seed with a pesticidal ie. insecticidal and fungicidal combination helps reduce damage from such soil-borne pests. Another advantage of treating seeds or other plant propagation material is the improvement in germination rates, increased yield, and improved plant health.
There are many combinations of insecticide and fungicide known in the art for the control of soil borne pests.
US5877194A discloses combinations for protecting plants comprising both a fungicidal active material and an insecticidal active material and to a process for treating plants using these combinations.
US7732374B2 discloses combinations of Prothioconazole and insecticides for the control of pests in seeds, soils etc.
There is however an ever increasing need for novel broad spectrum pesticidal combinations that not only provide protection against the pests ie. insect or fungi but also lead to increased vigor and strength in the germinated crops. Further, historic use of single active ingredient compositions has resulted in development of resistance. With the onset of resistance in certain pests, there is a need in the art for novel combination of actives that decreases chances of resistance and improves the spectrum of disease and pest control while also providing increased crop vigor. The present invention aims at providing such a seed treatment composition comprising selected insecticidal-fungicidal combination for use in variety of agricultural crops particularly in Gram (Chick pea) crop.
OBJECTS OF THE INVENTION:
An object of the present invention is to provide a novel and synergistic seed treatment composition that provides effective control of soil borne insects and fungal diseases while also improving the germination and enhancing the crop vigor.
It is another object of the present invention to provide a novel seed treatment composition comprising a combination of Pyraclostrobin, Thiamethoxam and Soy-protein which provides effective control of soil borne insects and fungal diseases while also improving and enhancing the germination and crop vigor, particularly in Gram (Chick pea)
It is yet another object of the present invention to provide a chemically stable synergistic seed treatment composition which is preferably formulated as a Flowable concentrate (FS).
It is yet another object of the present invention to provide a novel, synergistic seed treatment composition which has a broad spectrum of activity, especially against insect and fungi.
It is yet another object of the present invention to provide a novel, synergistic seed treatment composition that is non-phytotoxic.
SUMMARY OF THE INVENTION
The present invention relates to a novel and synergistic seed treatment composition that provides effective and simultaneous control of soil borne insects and fungal diseases in variety of agricultural crops, particularly gram (Chickpea).
Specifically, the present invention relates to seed treatment composition comprising Pyraclostrobin as the first component, Thiamethoxam as the second component and Soy protein as the third component in specific weight percentages along with at least one agricultural excipient. The present invention further relates to a process for the preparation of said novel and synergistic seed treatment composition.
In one aspect, the present invention relates to a novel, synergistic seed treatment composition comprising a) Pyraclostrobin in an amount ranging from 5-25 % of the pesticidal composition b) Thiamethoxam in an amount ranging from 15-30% of the pesticidal composition c) Soy -protein in an amount ranging from 0.1-10% of the pesticidal composition d) at least one agriculturally acceptable excipient.
Another aspect of the present invention relates to a process for preparation of said novel, synergistic seed treatment composition comprising the specified steps.
Yet another aspect of the present invention relates to a novel, synergistic pesticidal composition, wherein said pesticidal composition is preferably in the form of a liquid formulation.
Yet another aspect of the present invention relates to a novel, synergistic pesticidal composition, wherein said pesticidal composition is preferably formulated as a Flowable concentrate (FS)
DETAILED DESCRIPTION OF THE INVENTION
Definitions:
The following terms have the indicated meanings when used herein:
As used herein, the term "plant propagation material" refers to the parts of the plant, such as seeds, which can be used for the propagation of the plant and vegetative plant material such as cuttings and tubers (for example, potatoes). There may be mentioned, e.g., the seeds (in the strict sense), roots, fruits, tubers, bulbs, rhizomes, parts of plants.
As used herein, germinated plants or young plants, which may be transplanted after germination or after emergence from the soil.
As used herein, the term “Insecticidal" refers to the ability of a substance to increase mortality or inhibit, growth rate of insects. “Fungicidal” refers to the ability of a substance to decrease or inhibit growth of fungi.
As used herein, the term “agriculturally acceptable amount of active” refers to an amount of an active that kills or inhibits the plant disease for which control is desired, in an amount not significantly toxic to the plant being treated.
As used herein, “ to control” or “controlling” insects means to inhibit, through a toxic effect, the ability of insect pests to survive, grow, feed, and/or reproduce, or to limit insect-related damage or loss in crop plants or denotes control and prevention of a disease.
As used herein, the term “agriculturally acceptable amount of active” refers to an amount of an active that kills or inhibits the plant disease for which control is desired, in an amount not significantly toxic to the plant being treated. As used herein, the terms “comprise”, “comprising”, “include”, “includes”, and “including” are meant to be non-limiting i.e. other steps and other ingredients which do not affect the end of result can be added. The above terms encompass the terms “consisting of” and “consisting essentially of”.
Pyraclostrobin is a broad-spectrum fungicide belonging to a group which is collectively known as “Strobilurins”. It controls major plant pathogens such as belonging to ascomycetes, basidiomycetes, deuteromycetes and oomycetes classes of fungi. It is mainly used in crops like Bananas, barley, berries, potatoes, carrots, grape fruits, lemons, lime etc.
Thiamethoxam is a systemic insecticide belonging to the class of neonicotinoids. It has a broad spectrum of activity against many types of insects. It is a contact and stomach poison. It is particularly effective as a seed treatment against soil-dwelling and early season pests. As a seed treatment, the product can be used on a greater number of crops (including cereals) against a broader range of pests.
Soy protein basically serve as plant-derived biostimulants which are readily available in the markets. Traditionally, it is made from soybean meal that has been dehulled and defatted. Soyprotein extract contains various amino acids – Aspartic, Threonine, Serine, Glutamic acid, Proline, Glycine, Valine, Cystine, Isoleucine, Methionine, Tyrosine, Phenylalanine, Lysine, Histidine, Arginine, and the percentage of each amino acid may vary in the mixture. Soy protein has a bio-stimulating effect on the health of the crops.
In an embodiment, the present invention relates to a novel, synergistic seed treatment composition for control of soil borne insect (Termites) and fungal disease (Wilt disease) in variety of agricultural crops, particularly Gram (Chick pea)
In a specific embodiment, the present invention relates to a novel, synergistic seed treatment composition comprising Pyraclostrobin as its first component, Thiamethoxam as its second component and Soy protein as its third component, wherein the first component, Pyraclostrobin is present in an amount ranging from 5-25% w/w of the combination, wherein the second component Thiamethoxam is present in an amount ranging from 15-30% w/w of the combination and the third component, Soy protein is present in an amount ranging from 0.1 to 10% w/w of the combination. In a specific embodiment, the invention further relates to a process for preparing said composition.
Surprisingly it was observed by the inventors that the present composition provides a superior and a broad spectrum of control of soil borne and fungal disease at much lower levels of active ingredient combination as compared to what is achieved when the active ingredients are applied alone. Further, it was observed that the application of the presently disclosed seed treatment composition not only achieves effective control of soil borne insects and fungal diseases but also results in substantial improvement in germination and crop vigor.
In an embodiment, the present invention discloses a seed treatment composition which may be formulated in a form selected from the group comprising suspension concentrates, highly concentrated aqueous suspensions, wet dispersible powders, water dispersible granules, flowable concentrates, dispersions and aqueous solutions.
In an embodiment, the present invention discloses a seed treatment composition which may be formulated into any suitable formulation selected from the group comprising wet dispersible granules, flowable concentrate, suspensions, emulsions and Flowable concentrate (FS).
In a preferred embodiment, the present invention discloses a seed treatment composition which is formulated as a liquid formulation.

In a more preferred embodiment, the present invention discloses a seed treatment composition which may be formulated as a Flowable concentrate (FS). Flowable concentrates (FS) are suspensions of solid active ingredients in water which are applied to seeds prior to planting. This formulation is basically a modification of suspension concentrate (SC) and is currently most popular type of seed treatment as it is water based and safer to apply. Also, since it is a liquid formulation, there are no dust problems and seed treatment machinery is easy to clean up post- treatment and hence confers many advantages over traditional formulations.
In an embodiment, the Flowable concentrates of the present invention comprise an anti-freeze agent, a dispersing agent, a defoaming agent, a thickener, an antibacterial agent, and a suitable carrier.
In an embodiment, the present composition comprises one or more dispersing agents, wherein said dispersing agent is selected from the group comprising sodium lignosulphonates, sodium naphthalene sulphonate- formaldehyde condensates, aliphatic alcohol ethoxylates, tristyrylphenol ethoxylates and esters, ethylene oxide/propylene oxide block copolymers. Preferably the dispersing agent is tri steryl phenol ethoxylate potassium salt.
In an embodiment, the present composition comprises one or more wetting agents, wherein said wetting agent is selected from the group comprising blend of alkyl naphthalene sulfonate, sodium salt, Sodium laurel sulphate. In a preferred embodiment, the wetting agent is amine salt of poly aryl phenyl ether phosphate.
In an embodiment, said one or more dispersing or wetting agent may be present in an amount ranging from 3 to 6% w/w of the composition.

In an embodiment, the present composition comprises an anti-freeze agent which may be selected from the group comprising ethylene glycol, propylene glycol or glycerol.

In an embodiment, said anti-freeze agent is present in an amount ranging from 5 to 8% w/w of the composition.
In an embodiment, the present composition comprises a deformer or anti-foaming agent which may be silicon based. In an embodiment, said deformer or antifoaming agent is present in an amount ranging from 0.1 to 0.5% w/w of the composition.
In an embodiment, the present composition comprises one or more anti-bacterial or biocides. In an embodiment, said biocide or anti-bacterial agent is formaldehyde which may be present in an amount ranging from 0.1 to 0.5% w/w/of the composition.

In an embodiment, the present composition comprises one or more thickeners, wherein said thickeners is selected from the group comprising alginin, guar gum, locust bean gum, and xanthan gum.
In an embodiment, said one or more thickener may be present in an amount ranging from 0.1 to 0.3% w/w/of the composition.
In an embodiment, the Flowable concentrate (FS) composition of the present invention comprises supplemental additives for adhesion to the seed surface and colorants as safety markers to indicate that a seed has been treated with a product.
In an embodiment, the Flowable concentrate (FS) composition of the present invention comprises a sticker wherein said sticker is preferably PEG 400 wherein said sticker is present in an amount ranging from 2-6% w/w of the composition.
In an embodiment, the present composition comprises a red pigmented dye. In an embodiment, said red dye is present in an amount 2 to 5% w/w of the composition.

Flow Diagram representing the steps in preparation of CS 2015 FS

Check for particle size Sample sent for Quality check

EXAMPLES:
The examples below are given solely for the purpose of illustration and are not to be construed as limitations of the present invention, as many variations thereof are possible without departing from the spirit and scope of the invention.
EXAMPLE 1: Preparation of combination of the present invention:
In an embodiment, the seed treatment composition of the present invention was prepared by following the steps as provided below:
STEP 1: The mixture of surfactants and anti-freezing agent were first diluted in demineralized water and solubilized by high speed mixer and then deformer or anti foaming agent was added. This was followed by addition of Thiamethoxam technical. This was mixed properly to result in a homogeneous mixture. Following this, Pyraclostrobin technical and rest of the adjuvants were added in the required proportion.
STEP 2: The above mixed mass was subjected to grinding in a sand mill. Grinding was carried out until a mean particle size of below 5 micron was obtained.
STEP 4. The preparation of 2% gum solution was carried out by taking 10 gm of Demineralized water and adding 0.5 gm formaldehyde to it followed by slow stirring for 30 minutes. The solution was stored overnight.
STEP 5. After the grinding step, the desired amount of 2% water solution of xanthan gum was added into the homogenous mixture under slow stirring.
STEP 5: Finally, quality parameters were checked.
Table 1 (a): Chemical composition of the present invention (in wt. percentage range)
S. No. Chemical constituent % combination (w/w)
1 Thiamethoxam technical (Basis of 100.0%) 15-30%
2 Pyraclostrobin technical (Basis of 100.0%) 5-25%
3 Soya Protein 0.1-10%
4 Sticker (PEG 400) 2-6%
5 Anti-freeze agent (propylene glycol) 5-8%
6 Dispersing/ Wetting agent 3-6%
7 Deformer (silicon based) 0.1-5%
8 Anti-bacterial or Biocide 0.1-5%
9 Red dye 2-5%
10 Thickener (Xanthan gum) 0.1-0.3 %
11 D. M. Water Q. S.
Total quantity 100 % w/w

Table 1 (b): Chemical composition of the present invention (in wt. percentage)
S. No. Chemical constituent % combination (w/w)
1 Thiamethoxam technical (Basis of 100.0%) 20.00%
2 Pyraclostrobin technical (Basis of 100.0%) 15.00%
3 Soya Protein 00.50%
4 Sticker (PEG 400) 03.00%
5 Anti-freeze agent (propylene glycol) 06.00%
6 Dispersing/ Wetting agent 05.00%
7 Deformer (silicon based) 00.50%
8 Anti-bacterial or Biocide 00.20%
9 Red dye 03.00%
10 Thickener (Xanthan gum) 00.20%
11 D. M. Water Q. S.
Total quantity 100 % w/w

Table 2: Quality parameter of the formulation
S.no. Quality Parameter Desired quality
1 Appearance Material shall be in the form of free-flowing suspension
2 Pourability Residue shall not be more than 5% w/w.

3. Spontaneity of dispersion Shall not be less than 95% w/w
4. Wet sieve test 98% min (passes through 45-micron sieve).
5. Persistent foam Persistent of foam shall not be more than 60 ml in 1 min.
6. Suspensibility Not less than 95% w/w
7. pH of 1% aq. Solution 6.0-8.0
8. HST Take 100ml sample & store it in 54°C±2 for 14 days
9. Cold Test Take 100ml sample & store it in 5°C±2 for 14 days
10. Active ingredient content Thiamethoxam 20%
Pyraclostrobin 15%
Soy protein 0.5%

EXAMPLE 2: Evaluation of bioefficacy of the present composition
The presently disclosed seed treatment composition coded as 2015 FS was tested for its bioefficacy against Wilt disease and termite disease on gram (Chickpea) crop during the Rabi season 2019-20. The field trial was conducted on a local variety at Indore, India. The plants were aligned in a plot size of 100 sq. m., having a spacing of 15 cm between the individual crop plants and a spacing of 20 cm between the rows. The experiment was laid out in a random block design consisting of a total of 10 treatments in three replications. Further, the trial was conducted at a temperature of 27.5? and at a relative humidity of 75% and no winds and with optimum soil moisture. Measured quantity of the formulations was added to the required volume of water for seed treatment application. The solution was properly mixed with required quantity of seeds and thorough coverage of seeds was ensured.
TREATMENT DETAILS:
Table 3 below provides the treatment details of the Gram crop under the present experiment. For purposes of data presentation, the present composition has been represented or coded as CS- 2015 FS.
Table 3: Treatment details Gram- Seed rate- 80 Kg/ha
Treatments Treatment details Dose (gm) a.i./ha Dose (gm/ml)/ha Dose/Kg of seed
T-1 Untreated - - -
T-2 CCP-2015 FS (Sample-1) 43.4 + 56 280 ml 3.5 ml
T-3 CCP-2015 FS 49.6 + 64 320 ml 4 ml
T-4 CCP-2015 FS 55.8 +72 360 ml 4.5 ml
T-5 CCP-2015 FS 62 + 80 400 ml 5.0 ml
T-6 CCP-2015 FS (Sample-2) 43.4 + 56 + 1.4 280 ml 3.5 ml
T-7 CCP-2015 FS 49.6 + 64 + 1.6 320 ml 4 ml
T-8 CCP-2015 FS 55.8 +72 +1.8 360 ml 4.5 ml
T-9 CCP-2015 FS 62 +80 +2.0 400 ml 5.0 ml
T-10 Thiamethoxam 30 FS 72 240 ml 3 ml
T-11 Pyraclostrobin 20 FS 96 480 ml 6 ml
T2 -T5 represents Sample-1 CCP-2015 FS Pyraclostrobin 15.5% + Thiamethoxam 20%
T6 - T9 represents Sample-2 CCP-2015 FS Pyraclostrobin 15.5% + Thiamethoxam 20% + Soy Protein 0.5% FS
Note: DAA – Days after application, a.i – Active ingredient, ha-hectare,

EVALUATION OF BIO EFFICACY IN GRAM CROP:
For Disease incidence and termite incidence

a. Count the number of healthy and diseased plants from 0.25 sqm at 30 and 60 days after application

Percent control: Per-cent control of the disease incidence was calculated based on no. of damage plants. Formula are given below.

C – T
Percent control = …………. × 100
C

Where C = No. of diseased plants in control plot
T = No. of total plants in treated plot.

Observations:
Table 3(a): Bioefficacy of CS-2015 FS in Germination and root length of the Gram Crop
Treat. Treatment details Dose
gm a.i./ha Germination/Meter
(20 DAA) %
Increase Root length
(cm) %
Increase
T-1 Untreated - 9.00 - 6.70 -
T-2 CCP-2015 FS (Sample-1) 43.4 + 56 9.67 7.41 7.07 5.47
T-3 CCP-2015 FS 49.6 + 64 9.67 7.41 7.13 6.47
T-4 CCP-2015 FS 55.8 +72 9.67 7.41 7.17 6.97
T-5 CCP-2015 FS 62 + 80 9.82 9.11 7.19 7.31
T-6 CCP-2015 FS (Sample-2) 43.4 + 56 + 1.4 10.00 11.11 7.30 8.96
T-7 CCP-2015 FS 49.6 + 64 + 1.6 10.33 14.81 7.47 11.44
T-8 CCP-2015 FS 55.8 +72 +1.8 10.33 14.81 7.47 11.44
T-9 CCP-2015 FS 62 +80 +2.0 10.33 14.81 7.53 12.44
T-10 Thiamethoxam 30 FS 72 9.67 7.41 7.07 5.47
T-11 Pyraclostrobin 20 FS 96 9.67 7.41 7.23 7.96
Sample-1 CCP-2015 FS Pyraclostrobin 15.5% + Thiamethoxam 20%
Sample-2 CCP-2015 FS Pyraclostrobin 15.5% + Thiamethoxam 20% + Soy Protein 0.5% FS

Table 3(b): Bioefficacy of CS-2015 WG in test weight and Plot yield of the Gram Crop
Treat. Treatment details Dose gm a.i./ha Test weight (gm) % Increase Yield/ha (q) % Increase
T-1 Untreated - 26.30 - 9.90 -
T-2 CCP-2015 FS (Sample-1) 43.4 + 56 26.53 0.87 10.40 5.05
T-3 CCP-2015 FS 49.6 + 64 26.50 0.76 10.46 5.66
T-4 CCP-2015 FS 55.8 +72 26.64 1.29 10.62 7.27
T-5 CCP-2015 FS 62 + 80 26.59 1.10 10.80 9.09
T-6 CCP-2015 FS (Sample-2) 43.4 + 56 + 1.4 26.65 1.33 10.84 9.49
T-7 CCP-2015 FS 49.6 + 64 + 1.6 26.69 1.48 10.88 9.90
T-8 CCP-2015 FS 55.8 +72 +1.8 26.72 1.60 10.80 9.09
T-9 CCP-2015 FS 62 +80 +2.0 26.69 1.48 10.88 9.90
T-10 Thiamethoxam 30 FS 72 26.54 0.91 10.70 8.08
T-11 Pyraclostrobin 20 FS 96 26.51 0.80 10.36 4.65

Table 3(c): Bioefficacy of Seed treatment CS-2015 FS on Gram against Wilt (No. of damaged plants)
Treat. Treatment details Dose gm a.i. /ha 30 DAA % Control 60 DAA % Control
T-1 Untreated - 30.67 - 38.67 -
T-2 CCP-2015 FS (Sample-1) 43.4 + 56 11.00 64.13 12.00 68.97
T-3 CCP-2015 FS 49.6 + 64 9.33 69.57 9.90 74.39
T-4 CCP-2015 FS 55.8 +72 8.76 71.43 8.98 76.77
T-5 CCP-2015 FS 62 + 80 8.46 72.41 9.45 75.87
T-6 CCP-2015 FS (Sample-2) 43.4 + 56 + 1.4 8.67 71.74 9.33 75.86
T-7 CCP-2015 FS 49.6 + 64 + 1.6 7.67 75.00 7.33 81.03
T-8 CCP-2015 FS 55.8 +72 +1.8 6.67 78.26 7.00 81.90
T-9 CCP-2015 FS 62 +80 +2.0 6.67 78.26 7.00 81.90
T-10 Thiamethoxam 30 FS 72 11.00 64.13 12.00 68.97
T-11 Pyraclostrobin 20 FS 96 23.67 22.83 27.67 28.45

Table 3 (d): Bioefficacy of Seed treatment CS-2015 FS on Gram against Termites (No. of damaged plants)
Treat. Treatment details Dose gm a.i./ha 30 DAA % Control 60 DAA % Control
T-1 Untreated - 10 - 19 -
T-2 CCP-2015 FS (Sample-1) 43.4 + 56 4 60.00 8 57.89
T-3 CCP-2015 FS 49.6 + 64 3 70.00 5 73.68
T-4 CCP-2015 FS 55.8 +72 2 80.00 3 84.21
T-5 CCP-2015 FS 62 + 80 0 100.00 2 89.47
T-6 CCP-2015 FS (Sample-2) 43.4 + 56 + 1.4 3 70.00 4 79.00
T-7 CCP-2015 FS 49.6 + 64 + 1.6 0 100.00 1 94.73
T-8 CCP-2015 FS 55.8 + 72 + 1.8 0 100.00 0 100.00
T-9 CCP-2015 FS 62 + 80 + 2.0 0 100.00 0 100.00
T-10 Thiamethoxam 30 FS 72 3 70.00 4 79.00
T-11 Pyraclostrobin 20 FS 96 7 30.00 14 26.31

EXAMPLE 3: Evaluation of phytotoxicity of Seed treatment CS-2015 FS on Gram
Visual observations were recorded at 10, 20, 30 and 50 days after application (DAA) of testing the presently disclosed composition and certain other reference products. The parameters observed were leaf injury on tip/surface, necrosis, vein clearing, epinasty, hyponasty and wilting. The score scale (1-10) followed for leaf injury on tips/surface is depicted in the table below:
Table 4: Phytotoxicity symptoms scoring and rating for leaf injury on tip/surface
Leaf injury on tips/surface Rating
0% 0
1-10% 1
11-20% 2
21-30% 3
31-40% 4
41-50% 5
51-60% 6
61-70% 7
71-80% 8
81-90% 9
91-100% 10

Table 5. The Observation were recorded as given below:
Treatment Treatment details Dose gm a.i./ha 10 DAA 20 DAA 30 DAA 50 DAA
T-1 Untreated - 0 0 0 0
T-2 CCP-2015 FS (Sample-1) 43.4 + 56 0 0 0 0
T-3 CCP-2015 FS 49.6 + 64 0 0 0 0
T-4 CCP-2015 FS 55.8 +72 0 0 0 0
T-5 CCP-2015 FS 62 + 80 0 0 0 0
T-6 CCP-2015 FS (Sample-2) 43.4 + 56 + 1.4 0 0 0 0
T-7 CCP-2015 FS 49.6 + 64 + 1.6 0 0 0 0
T-8 CCP-2015 FS 55.8 +72 +1.8 0 0 0 0
T-9 CCP-2015 FS 62 +80 +2.0 0 0 0 0
T-10 Thiamethoxam 30 FS 72 0 0 0 0
T-11 Pyraclostrobin 20 FS 96 0 0 0 0
Note: DAA – Days after application L- Leaf injury on tips/surface, N-Necrosis, V- Vein
clearing, E- Epinasty, H- Hyponasty, W-wilting.
Result and observations
Data in Tables 3(a)(b)(c) & (d) as provided above indicated that maximum % increase in terms of parameters like root length (cm) and germination of Chick pea (in 1 sq.m. area) was observed by application of CCP-2015 FS (Sample-2- Pyraclostrobin 15.5% + Thiamethoxam 20% + Soy protein 0.5% ) at a dosage of 49.6 + 64 + 1.6 (4 ml per kg of seed). The formulation showed high percent control of Fusarium wilt and termite pest in Chick pea crop accompanied with increased crop vigor and no or negligible phytotoxicity as evident from data in Table 5.
While certain embodiments have been described, these embodiments have been presented by way of example only, and are not intended to limit the scope of the present disclosure. Indeed, the novel methods, devices, and systems described herein may be embodied in a variety of other forms. Furthermore, various omissions, substitutions, and changes in the form of the methods, devices, and systems described herein may be made without departing from the spirit of the present disclosure.