FIELD OF THE INVENTION
[0001] The present invention relates to a self-disinfecting composition. Specifically, the present invention relates to a harsh chemical-free (i.e. alcohol, hypochlorite, acid, and toxic chemical free) self-disinfecting composition having antimicrobial, antibacterial, antifungal, antiviral properties. The present invention further relates to a harsh chemical-free self-disinfecting composition comprising a combination of metal and nanoparticles of a herbal organic compound; and a method for producing the same.
BACKGROUND OF THE INVENTION
[0002] Background description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art. [0003] Sanitizing or disinfecting is a critical component in limiting or preventing the transmission of many diseases. Sanitizer compositions have become a global phenomenon and have developed into larger market, especially after the surge of COVID-19 pandemic. These sanitizing compositions rely on thousands of different synthetic chemicals employed in personal care products purported for use on human skin. Most of these chemicals are actually found to cause adverse health risks but are still being used.
[0004] Conventional disinfectants used as antimicrobial agents have harmful ingredients like chemicals and solvents. With the advent of Nanotechnology, the goal of chemical-free disinfectants can be achieved. Nanoparticles prepared from biopolymers or certain metals having antimicrobial properties have several advantages over conventional chemical disinfectants like negligible toxicity, lacking harmful solvents or chemicals components.
[0005] The prior arts available in this field are generally composed of alcohol with one or two active ingredients and lacking cleansing action. Alcohol itself causes skin irritation and rashes making it a secondary choice. Additionally, the effectiveness of the prior arts has been compromised due to short-term activity.

[0006] People nowadays use alcohol-based hand sanitizers and disinfectants in a variety of ways, but they are no long lasting. Since they are toxic chemical based, they cause skin problems such as lesions, rashes, rough skin, and irritation. Furthermore, long-term use of these alcohol, acids, and hypochlorite-based products can cause internal health concerns in children.
[0007] Thus, there is a great need in the art for pharmaceutical composition that is free of alcohol and hypochlorite, and comprises natural and bio-safe ingredients for effective and long-lasting action against a broad spectrum of microorganisms in comparison to prior arts.
[0008] The present disclosure overcomes the deficiencies of the art by providing a non-toxic eco-friendly compositions which are effective against a broad spectrum of microorganisms.
[0009] The compositions of the present invention provide an effective anti-microbial composition that is active against a host of bacteria, including MRSA, and viruses. Said composition also provides a soothing effect and is suitable for all skin types. The present invention additionally provides the advantage of being capable of use for skin disinfection.
[0010] The present invention satisfies the existing needs, as well as others, and generally overcomes the deficiencies found in the prior art.
OBJECTS OF THE INVENTION
[0011] An object of the present invention is to provide a self-disinfecting
composition comprising a combination of metal nanoparticles and nanoparticles of
a herbal organic compound.
[0012] An object of the present invention is to provide a harsh chemical-free
self-disinfecting composition comprising a combination of metal nanoparticles and
nanoparticles of a herbal organic compound.
[0013] An object of the present invention is to provide a harsh chemical-free
hard surface self-disinfecting composition comprising a combination of metal
nanoparticles and nanoparticles of a herbal organic compound.

[0014] An object of the present invention is to provide a harsh chemical-free self-disinfecting composition a combination of metallic nanoparticles and biopolymer encapsulated nanoparticles of a herbal organic compound. [0015] An object of the present invention is to provide a harsh chemical-free self-disinfecting composition comprising excellent antimicrobial, antibacterial, antifungal, antiviral activities.
[0016] An object of the present invention is to provide a harsh chemical-free self-disinfecting composition that is effective even on Methicillin-Resistant Staphylococcus Aureus (MRS A).
[0017] An object of the present invention is to provide a harsh chemical-free self-disinfecting composition that is effective against all types of viruses. [0018] An object of the present invention is to provide a harsh chemical-free self-disinfecting composition comprising nanoparticles suspended in pharmaceutical grade water or double distilled water comprising non-ionic surfactant, wherein the nanoparticles comprise a synergistic combination of metal nanoparticles and biopolymer encapsulated nanoparticles of a herbal organic compound. [0019] An object of the present invention is to provide a harsh chemical-free self-disinfecting composition comprising a synergistic combination of titanium nanoparticles, silver nanoparticles, and PLA encapsulated curcumin nanoparticles suspended in pharmaceutical grade water or double distilled water comprising poloxamer.
[0020] An object of the present invention is to provide a harsh chemical-free self-disinfecting composition comprising a synergistic combination of Ti02 nanoparticles, silver nanoparticles and PLA encapsulated curcumin nanoparticles suspended in pharmaceutical grade water or double distilled water comprising poloxamer.
[0021] An object of the present invention is to provide a lyophilized harsh chemical-free self-disinfecting composition comprising a synergistic combination of Ti02 nanoparticles, silver nanoparticles and PLA encapsulated curcumin nanoparticles suspended in pharmaceutical grade water or double distilled water comprising poloxamer.

[0022] Another object of the present invention is to provide a method for preparing a harsh chemical-free self-disinfecting composition comprises the steps of:
a. preparing a solution of non-ionic surfactant in glass beaker using
pharmaceutical grade water on a magnetic stirrer;
b. preparing a solution of biopolymer in acetonitrile in a separate glass
vial;
c. adding nanoparticles of a herbal organic compound to biopolymer
solution from step b), followed by brief sonication;
d. adding the mixture from step c) to aqueous phase from step a) under
continuous overnight stirring using magnetic stirrer to evaporate the
organic solution;
e. adding metallic nanoparticles to final solution from step d), followed
by mixing with magnetic stirrer.
[0023] Another object of the present invention is to provide a method for preparing a harsh chemical-free self-disinfecting composition comprises the steps of:
a. preparing a solution of poloxamer in glass beaker using
pharmaceutical grade water on a magnetic stirrer;
b. preparing a solution of polylactic acid (PLA) in acetonitrile in a
separate glass vial;
c. adding curcumin to PLA solution from step b), followed by brief
sonication for 15 seconds;
d. adding the mixture from step c) to aqueous phase from step a) under
continuous overnight stirring using magnetic stirrer to evaporate the
organic solution;
e. adding Ti02 and silver nanoparticles to final solution from step d),
followed by mixing with magnetic stirrer.
[0024] Yet another object of the present invention is to provide a harsh chemical-free self-disinfecting composition that is suitable for all skin types.

[0025] Yet another object of the present invention is to provide a harsh chemical-free self-disinfecting composition that has no irritation on the skin and eye of the individual.
[0026] Yet another object of the present invention is to provide a harsh chemical-free self-disinfecting composition that is suitable for sanitizing and disinfecting hard surfaces instantly.
[0027] Yet another object of the present invention is to provide a harsh chemical-free self-disinfecting composition that can be used in home as well as office, hospitality, transport, and hospital settings.
[0028] Yet another object of the present invention is to provide a harsh chemical-free self-disinfecting composition that can be used for sanitizing and disinfecting all type of surfaces including, metal, wooden, marble, granite sun-mica, glass, leather, rexine, plastic, iron, and the like.
[0029] Yet another object of the present invention is to a harsh chemical-free self-disinfecting composition that is biodegradable and environment-friendly. [0030] Yet another object of the present invention is to a harsh chemical-free self-disinfecting composition that can disinfect the surface within 30 seconds of application and can retain disinfectant property even after 96 hrs after application. [0031] Yet another object of the present invention is to a harsh chemical-free self-disinfecting composition that has disinfecting activity even after 9 days after application.
[0032] Yet another object of the present invention is to provide a harsh chemical-free self-disinfecting composition that is Non-irritant/dermatologically safe and dermatologically tested: safe for human skin for long-term use.
SUMMARY OF THE INVENTION
[0033] This summary is provided to introduce a selection of concepts in a simplified form that is further described below in the detailed description section. This summary is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used as an aid in determining the scope of the claimed subject matter.

[0034] The present invention relates to a self-disinfecting composition. Specifically, the present invention relates to a harsh chemical-free self-disinfecting composition having antimicrobial, antibacterial, antifungal, antiviral properties. [0035] In an aspect, the present invention relates to a self-disinfecting composition comprising a combination of metal nanoparticles and nanoparticles of a herbal organic compound.
[0036] In an aspect, the present invention relates to a harsh chemical-free self-disinfecting composition comprising a combination of metal nanoparticles and nanoparticles of a herbal organic compound.
[0037] In an aspect, the present invention relates to a harsh chemical-free hard surface self-disinfecting composition comprising a combination of metal nanoparticles and nanoparticles of a herbal organic compound. [0038] In an aspect, the present invention relates to a harsh chemical-free self-disinfecting composition a combination of metallic nanoparticles and biopolymer encapsulated nanoparticles of a herbal organic compound.
[0039] In an aspect, the present invention relates to a harsh chemical-free self-disinfecting composition comprising excellent antimicrobial, antibacterial, antifungal, antiviral activities.
[0040] In an aspect, the present invention relates to a harsh chemical-free self-disinfecting composition that is effective even on Methicillin-Resistant Staphylococcus Aureus (MRS A).
[0041] In an aspect, the present invention relates to a harsh chemical-free self-disinfecting composition that is effective against all types of viruses. [0042] In an aspect, the present invention relates to a harsh chemical-free self-disinfecting composition comprising nanoparticles suspended in pharmaceutical grade water or double distilled water comprising non-ionic surfactant, wherein the nanoparticles comprise a synergistic combination of metal nanoparticles and biopolymer encapsulated nanoparticles of a herbal organic compound. [0043] In an aspect, the present invention relates to a harsh chemical-free self-disinfecting composition comprising a synergistic combination of titanium nanoparticles, silver nanoparticles, and PLA encapsulated curcumin nanoparticles

suspended in pharmaceutical grade water or double distilled water comprising poloxamer.
[0044] In an aspect, the present invention relates to a harsh chemical-free self-disinfecting composition comprising a synergistic combination of Ti02 nanoparticles, silver nanoparticles and PLA encapsulated curcumin nanoparticles suspended in pharmaceutical grade water or double distilled water comprising poloxamer.
[0045] In an aspect, the present invention relates to a lyophilized harsh chemical-free self-disinfecting composition comprising a synergistic combination of Ti02 nanoparticles, silver nanoparticles and PLA encapsulated curcumin nanoparticles suspended in pharmaceutical grade water or double distilled water comprising poloxamer.
[0046] In another aspect, the present invention relates to a method for preparing a harsh chemical-free self-disinfecting composition comprises the steps of:
a. preparing a solution of non-ionic surfactant in glass beaker using
pharmaceutical grade water on a magnetic stirrer;
b. preparing a solution of biopolymer in acetonitrile in a separate glass
vial;
c. adding nanoparticles of a herbal organic compound to biopolymer
solution from step b), followed by brief sonication;
d. adding the mixture from step c) to aqueous phase from step a) under
continuous overnight stirring using magnetic stirrer to evaporate the
organic solution;
e. adding metallic nanoparticles to final solution from step d), followed
by mixing with magnetic stirrer.
[0047] In another aspect, the present invention relates to a method for preparing a harsh chemical-free self-disinfecting composition comprises the steps of:
a. preparing a solution of poloxamer in glass beaker using pharmaceutical grade water on a magnetic stirrer;

b. preparing a solution of polylactic acid (PLA) in acetonitrile in a
separate glass vial;
c. adding curcumin to PLA solution from step b), followed by brief
sonication for 15 seconds;
d. adding the mixture from step c) to aqueous phase from step a) under
continuous overnight stirring using magnetic stirrer to evaporate the
organic solution;
e. adding Ti02 and silver nanoparticles to final solution from step d),
followed by mixing with magnetic stirrer.
[0048] In yet another aspect, the present invention relates to a harsh chemical-free self-disinfecting composition that is suitable for all skin types. [0049] In yet another aspect, the present invention relates to a harsh chemical-free self-disinfecting composition that has no irritation on the skin and eye of the individual.
[0050] In yet another aspect, the present invention relates to a harsh chemical-free self-disinfecting composition that is suitable for sanitizing and disinfecting hard surfaces instantly.
[0051] In yet another aspect, the present invention relates to a harsh chemical-free self-disinfecting composition that can be used in home as well as office, hospitality, transport, and hospital settings.
[0052] In yet another aspect, the present invention relates to a harsh chemical-free self-disinfecting composition that can be used for sanitizing and disinfecting all type of surfaces including, metal, wooden, marble, granite sun-mica, glass, leather, rexine, plastic, iron, and the like.
[0053] In yet another aspect, the present invention relates to a harsh chemical-free self-disinfecting composition that is biodegradable and environment-friendly. [0054] In yet another aspect, the present invention relates to a harsh chemical-free self-disinfecting composition that can disinfect the surface within 30 seconds of application and can retain disinfectant property even after 96 hrs after application.

[0055] In yet another aspect, the present invention relates to a harsh chemical-free self-disinfecting composition that has disinfecting activity even after 9 days after application.
[0056] In yet another aspect, the present invention relates to a harsh chemical-free self-disinfecting composition that is Non-irritant/dermatologically safe and dermatologically tested: safe for human skin for long-term use. [0057] Various objects, features, aspects, and advantages of the inventive subject matter will become more apparent from the following detailed description of preferred embodiments
BRIEF DESCRIPTION OF THE DRAWINGS
[0058] FIG. 1 represents the particle size distribution of PLA encapsulated
curcumin nanoparticles.
[0059] FIG. 2 represents the correlation function of PLA encapsulated
curcumin nanoparticles.
[0060] FIG. 3 represents the image of PLA encapsulated curcumin
nanoparticles.
[0061] FIG. 4A represents the images of microbial colony before treatment
with harsh chemical-free self-disinfecting composition.
[0062] FIG. 4B represents the images of antimicrobial activity of harsh
chemical-free self-disinfecting composition 15 minutes after treatment.
[0063] FIG. 4C represents the images of antimicrobial activity of harsh
chemical-free self-disinfecting composition after 48 hours after treatment.
[0064] FIG. 4D represents the images of antimicrobial activity of harsh
chemical-free self-disinfecting composition after 96 hours after treatment.
DETAILED DESCRIPTION OF THE INVENTION
[0065] The following is a detailed description of embodiments of the disclosure. The embodiments are in such detail as to clearly communicate the disclosure. However, the amount of detail offered is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all

modifications, equivalents, and alternatives falling within the spirit and scope of the present disclosure.
[0066] All publications herein are incorporated by reference to the same extent as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference. Where a definition or use of a term in an incorporated reference is inconsistent or contrary to the definition of that term provided herein, the definition of that term provided herein applies and the definition of that term in the reference does not apply.
[0067] Reference throughout this specification to "one embodiment" or "an embodiment" means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrases "in one embodiment" or "in an embodiment" in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
[0068] In some embodiments, numbers have been used for quantifying weight percentages, ratios, and so forth, to describe and claim certain embodiments of the invention and are to be understood as being modified in some instances by the term "about." Accordingly, in some embodiments, the numerical parameters set forth in the written description and attached claims are approximations that can vary depending upon the desired properties sought to be obtained by a particular embodiment. In some embodiments, the numerical parameters should be construed in light of the number of reported significant digits and by applying ordinary rounding techniques. Notwithstanding that the numerical ranges and parameters setting forth the broad scope of some embodiments of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as practicable. The numerical values presented in some embodiments of the invention may contain certain errors necessarily resulting from the standard deviation found in their respective testing measurements.

[0069] Various terms as used herein are shown below. To the extent a term used in a claim is not defined below, it should be given the broadest definition persons in the pertinent art have given that term as reflected in printed publications and issued patents at the time of filing.
[0070] As used in the description herein and throughout the claims that follow, the meaning of "a," "an," and "the" includes plural reference unless the context clearly dictates otherwise. Also, as used in the description herein, the meaning of "in" includes "in" and "on" unless the context clearly dictates otherwise. [0071] Unless the context requires otherwise, throughout the specification which follow, the word "comprise" and variations thereof, such as, "comprises" and "comprising" are to be construed in an open, inclusive sense that is as "including, but not limited to."
[0072] The recitation of ranges of values herein is merely intended to serve as a shorthand method of referring individually to each separate value falling within the range. Unless otherwise indicated herein, each individual value is incorporated into the specification as if it were individually recited herein. [0073] All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g. "such as") provided with respect to certain embodiments herein is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention otherwise claimed. No language in the specification should be construed as indicating any non-claimed element essential to the practice of the invention. [0074] Groupings of alternative elements or embodiments of the invention disclosed herein are not to be construed as limitations. Each group member can be referred to and claimed individually or in any combination with other members of the group or other elements found herein. One or more members of a group can be included in, or deleted from, a group for reasons of convenience and/or patentability. When any such inclusion or deletion occurs, the specification is herein deemed to contain the group as modified.

[0075] The description that follows, and the embodiments described therein, is provided by way of illustration of an example, or examples, of particular embodiments of the principles and aspects of the present disclosure. These examples are provided for the purposes of explanation, and not of limitation, of those principles and of the disclosure.
[0076] It should also be appreciated that the present disclosure can be implemented in numerous ways, including as a system, a method or a device. In this specification, these implementations, or any other form that the invention may take, may be referred to as processes. In general, the order of the steps of the disclosed processes may be altered within the scope of the invention. [0077] The headings and abstract of the invention provided herein are for convenience only and do not interpret the scope or meaning of the embodiments. [0078] The following discussion provides many example embodiments of the inventive subject matter. Although each embodiment represents a single combination of inventive elements, the inventive subject matter is considered to include all possible combinations of the disclosed elements. Thus if one embodiment comprises elements a, b, and c, and a second embodiment comprises elements b and d, then the inventive subject matter is also considered to include other remaining combinations of a, b, c, or d, even if not explicitly disclosed. [0079] While a particular form of the invention has been illustrated and described, it will be apparent that various modifications can be made without departing from the spirit and scope of the invention.
[0080] Unless the context requires otherwise, throughout the specification which follow, the expression "nano sized particles" and variations thereof, such as, "nanoparticles" and "nano shaped particles" relate mainly to the "nanoparticles". [0081] As described herein, the term "microorganism" as used herein refers to bacteria (gram positive and gram negative), anaerobes, yeasts, viruses, fungi, mold, prions and the like.
[0082] As described herein, the term "self-disinfecting" refers to a composition capable of inhibiting the growth or killing microorganisms such as bacteria (gram

positive and gram negative), anaerobes, yeasts, viruses, fungi, mold, prions and the like.
[0083] As described herein, the term "synergistic" as used herein refers to the effect where the antimicrobial activity of the combination of two compounds is more than the addition of the antimicrobial activity of the two compounds when tested alone.
[0084] The present invention relates to a self-disinfecting composition. Specifically, the present invention relates to a harsh chemical-free hybrid self-disinfecting composition comprising a combination of metal and nanoparticles of a herbal organic compound, and a method for producing the same. [0085] In an embodiment of the present disclosure, the harsh chemical-free self-disinfecting composition comprising a combination of metal and nanoparticles of a herbal organic compound, wherein the composition is synergistic composition. [0086] In an embodiment of the present disclosure, the harsh chemical-free self-disinfecting composition comprising a combination of metallic nanoparticles and nanoparticles of a herbal organic compound, wherein the composition is synergistic composition.
[0087] In an embodiment of the present disclosure, the harsh chemical-free self-disinfecting composition is biodegradable and environment-friendly. [0088] In an embodiment of the present disclosure, the harsh chemical-free self-disinfecting composition that is suitable for all skin types. [0089] In an embodiment of the present disclosure, the harsh chemical-free self-disinfecting composition that has a mild and gentle action on the skin and eye of the individual.
[0090] In an embodiment of the present disclosure, the harsh chemical-free self-disinfecting composition that is suitable for sanitizing and disinfecting hard surfaces instantly.
[0091] In an embodiment of the present disclosure, the harsh chemical-free self-disinfecting composition that can be used in home as well as office, hospitality, transport, and hospital settings.

[0092] In an embodiment of the present disclosure, the harsh chemical-free
self-disinfecting composition that can be used for sanitizing and disinfecting all
type of surfaces including, metal, wooden, marble, granite sun-mica, glass, leather,
rexine, plastic, iron, and the like.
[0093] In an embodiment of the present disclosure, the harsh chemical-free
self-disinfecting composition that can disinfect the surface within 30 seconds of
application and can retain disinfectant property even after 96 hrs after application.
[0094] In an embodiment of the present disclosure, the harsh chemical-free
self-disinfecting composition that has disinfecting activity even after 9 days after
application.
[0095] In an embodiment of the present disclosure, the harsh chemical-free
self-disinfecting composition forms a disinfecting layer on the surface on which it
has been applied.
[0096] In an embodiment of the present invention, the disinfection efficacy is at
least about 99.99% w/v, more usually at least about 99.00%, at least about 98.00%,
at least about 97.00%>, at least about 96.00%>, and not less than about 95.00%> or
about 90.00%o, but usually not less than about 90.00%>, or not more than about
85.00%. Most preferably, the disinfection efficacy is 90.00% to 99.99% based on
the time post-application.
[0097] In an embodiment of the present invention, the disinfection efficacy is at
least about 99.99%> during first half-an hour after application, and usually not less
than about 90.00%> at 96 hrs after application.
[0098] In an embodiment of the present disclosure, the harsh chemical-free
self-disinfecting composition that is Non-irritant/dermatologically safe and
dermatologically tested: safe for human skin for long-term use.
[0099] In an embodiment of the present disclosure, the composition reduces or
inhibits the growth of or kills microbes, wherein the microbes include but not
limited to green and blue-green algae, gram negative and gram positive bacteria,
enveloped and non-enveloped viruses, as well as fungi, including molds and yeasts.
[00100] In an embodiment of the present disclosure, the harsh chemical-free
self-disinfecting composition is effective against various microorganisms that are

effectively inhibited or killed by the composition of the invention include but are not limited to Aspergillus genus, Alcaligenes faecalis, Aureobasidium pullulans, Acremonium butryi, Bacillus cereus, Cephalosporium, Candida genus including Candida albicans, Chlorella spp, Chlorella vulgaris, Chaetomium globosum, Citrobacter freundii, Escherichia spp, Escherichia coli, Fusarim spp, Klebsiella pneumonia spp., Listeria spp., Malassezia spp, Malassezia furfur, Malassezia sympodialis, Malassezia globosa, Mycobacterium chelonae, Oscillitoria spp, Penicillium citrinum, Propionibacterium acne, Proteus mirabilis, Pseudomonas aeruginosa, Pseudomonas fluorescens, Pityrosporum ovale, Pseudomonas oleovorans, Pseudomonas rubescens, Pseudomonas stutzeri, Staphylococcus aureus including MRSA, Salmonella enteric, Shewanella putrefaciens, Streptococcus pyogenes, Staphylococcus epidermidis; those of the genus Trichophyton such as Trichophyton rubrum, Trichophyton mentagrophytes, Trichophyton verrucosum, Trichophyton tonsurans, Trichophyton equinum, Trichophyton kanei, Trichophyton raubitschekii, and Trichophyton violaceum; those of the genus Microsporum such as Microsporum gypseum, Microsporum audouinii, Microsporum nanum, Microsporum versicolor, Microsporum equinum, and Microsporum canis; Streptococcus species including Streptococcus oralis, Streptococcus mitis, Streptococcus pyogenes; Haemophilus species including Haemophilus influenzae, Haemophilus somnus; Actinobacillus species including Actinobacillus actinomycetemcomitans; Pseudomonas species including Pseudomonas aeruginosa; Fusobacterium species including Fusobacterium nucleatum; Actinomyces species; Neisseria species including Neisseria meningitides; Mycoplasma species including Mycoplasma fermentans and Mycoplasma pneumonia; viruses including but not limited to viruses causing infection at the respiratory tract, human papilloma virus (HPV), Respiratory Syncytial Virus (RSV), human metapneumovirus (hMPV), influenza A, influenza B, parainfluenza viruses, adenovirus, rhinoviruses, SARS virus including SARS-CoV and SARS-CoV-2, Coronaviruses including nCOVID-19, and the like.

[00101] In some embodiments, the present invention relates to a harsh chemical-free self-disinfecting composition wherein the composition further comprises an aromatic or fragrance generating agent.
[00102] In some embodiments, the present invention provides a harsh chemical-free self-disinfecting composition optionally including a pH regulator. [00103] In an embodiment of the present invention, the pH regulator can be selected from sodium hydroxide, citric acid, lactic acid, triethanolamine, glacial acetic acid, sodium carbonate, acetic acid, and the like.
[00104] In an embodiment of the present invention, the solvent is purified water, double distilled water or pharmaceutical grade water.
[00105] In an embodiment of the present invention, the fragrance is natural fragrance selected from lemon, jasmine, lavender, mint ice, peach, red berry, apple, vanilla, rose, rosemary, mogra, sandalwood, orange, and the like. [00106] In an embodiment of the present invention, the harsh chemical-free self-disinfecting composition may further comprise a biosurfactant in an amount ranging from about 0.001% w/v to about 10.0% w/v, by weight with respect to the overall composition.
[00107] In an embodiment of the present invention, the harsh chemical-free self-disinfecting composition may further comprise a pH regulator in an amount ranging from about 0.01% w/v to about 4.0% w/v, preferably in the amount ranging from about 0.01%) w/v to about 2.0% w/v by weight with respect to the overall composition.
[00108] In an embodiment of the present invention, the harsh chemical-free self-disinfecting composition may further comprise a natural colour in the amount ranging from about 0.01% w/v to about 1.0% w/v by weight with respect to the overall composition.
[00109] In an embodiment of the present invention, the harsh chemical-free self-disinfecting composition may further comprise a natural fragrance in the amount ranging from about 0.01% w/v to about 1.0% w/v by weight with respect to the overall composition.

[00110] In an embodiment of the present invention, the harsh chemical-free self-disinfecting composition may further comprise a solvent in a quantity sufficient to make up the weight percentage to 100% w/v.
[00111] In an embodiment of the present invention, the harsh chemical-free
self-disinfecting composition does not comprise acid, hypochlorite, ethanol, isopropyl alcohol, or any other toxic chemical purported to be used in conventional sanitizing or disinfecting compositions.
[00112] In another embodiment, the present invention provides a harsh chemical-free self-disinfecting composition comprising a synergistic combination of metallic nanoparticles and biopolymer encapsulated nanoparticles of a herbal organic compound.
[00113] In an embodiment of the present invention, metal nanoparticles include the nanoparticles of silicon, tin, titanium, aluminum, zinc, iron, nickel, manganese, magnesium, calcium, strontium, barium, copper, silver, tungsten, niobium, molybdenum, vanadium, zirconium, tantalum, boron, thorium, uranium, and combinations thereof. The form of the metal-containing nanoparticle can vary, but is typically selected from borides, carbides, nitrides, oxides, carbonitrides, silicides, sulfides, and combinations thereof. Preferably, the metal oxides include but not limited to oxide of transition metals, earth metals, metalloids, post-transition metals, and combinations thereof. Most preferably titanium dioxide (Ti02) and silver nanoparticles.
[00114] In an embodiment of the present invention, the silver nanoparticle is selected from but not limited to elemental silver or a silver salt. Suitable silver salts include silver dioxide, silver acetate, silver benzoate, silver carbonate, silver iodate, silver iodide, silver lactate, silver laurate, silver nitrate, silver oxide, silver palmitate, silver protein, silver sulfadiazine, and the like.
[00115] In an embodiment of the present invention, the harsh chemical-free
self-disinfecting composition comprises metallic nanoparticle at a concentration of at least about 0.01% w/v, more usually at least about 0.015% w/v, at least about 0.02%) w/v, at least about 0.025%> w/v, at least about 0.03%> w/v, and not more than about 0.035%) w/v or about 0.04% w/v, but usually not more than about 0.045%)

w/v, or not more than about 0.05% w/v. Most preferably, the percentage of metallic
nanoparticle is 0.025%) w/v with respect to the overall composition.
[00116] In an embodiment of the present invention, the harsh chemical-free
self-disinfecting composition comprises Ti02 nanoparticle at a concentration of at
least about 0.01%> w/v, more usually at least about 0.015%> w/v, at least about 0.02%
w/v, at least about 0.025%> w/v, at least about 0.03%> w/v, and not more than about
0.035%) w/v or about 0.04% w/v, but usually not more than about 0.045%) w/v, or
not more than about 0.05% w/v. Most preferably, the percentage of Ti02
nanoparticle is 0.025%) w/v with respect to the overall composition.
[00117] In an embodiment of the present invention, the harsh chemical-free
self-disinfecting composition comprises silver nanoparticle at a concentration of at
least about 0.01% w/v, more usually at least about 0.015% w/v, at least about 0.02%
w/v, at least about 0.025%> w/v, at least about 0.03% w/v, and not more than about
0.035%) w/v or about 0.04% w/v, but usually not more than about 0.045%) w/v, or
not more than about 0.05% w/v. Most preferably, the percentage of silver
nanoparticle is 0.025%) w/v with respect to the overall composition.
[00118] In an embodiment of the present invention, the harsh chemical-free
self-disinfecting composition comprises poloxamer at a concentration of at least about 0.1%) w/v, more usually at least about 0.2% w/v, at least about 0.5% w/v, at least about 1% w/v, at least about 1.5% w/v, and not more than about 2.0% w/v or about 2.5%o w/v, but usually not more than about 3.0%> w/v, or not more than about 5%> w/v. Most preferably, the percentage of poloxamer is 1% w/v with respect to the overall composition.
[00119] In an embodiment of the present invention, the harsh chemical-free
self-disinfecting composition comprises polylactic acid (PLA) at a concentration of at least about 0.1% w/v, more usually at least about 0.2% w/v, at least about 0.5% w/v, at least about 1% w/v, at least about 1.5% w/v, and not more than about 2.0% w/v or about 2.5% w/v, but usually not more than about 3.0%> w/v, or not more than about 5%> w/v. Most preferably, the percentage of PLA is 1% w/v with respect to the overall composition.

[00120] In an embodiment of the present invention, the "nanoparticles" have
sizes ranging in the nanometer scale. However, many modified nanoparticles have
wider ranges of sizes. In some embodiments, the nanoparticles may have a diameter
of at least about 1 nm, 10 nm, 50 nm, 100 nm, 200 nm, 300 nm, 400 nm, 500 nm,
600 nm, 700 nm, 800 nm, 900 nm, 1000 nm, 1100 nm, 1200 nm, 1300 nm, 1400
nm, 1500 nm, 1600 nm, 1700 nm, 1800 nm, 1900 nm, 2000 nm, 2500 nm, 3000
nm, 4000 nm, 5000 nm, 6000 nm, 7000 nm, 8000 nm, or at least 9000 nm. In some
embodiments, the nanoparticles may have a diameter of less than 10,000 nm, 9000
nm, 8000 nm, 7000 nm, 6000 nm, 5000 nm, 4500 nm, 4000 nm, 3500 nm, 3000
nm, 2500 nm, 2000 nm, 1900 nm, 1800 nm, 1700 nm, 1600 nm, 1500 nm, 1400
nm, 1300 nm, 1200 nm, 1100 nm, 1000 nm, 900 nm, 800 nm, 700 nm, 600 nm, 500
nm, 250 nm, or less than 100 nm. The diameter of nanoparticles can range from any
of the minimum values described above to any of the maximum values described
above, for example from 1 nm to 10,000 nm, 50 nm to 5,000 nm, 100 nm to 2500
nm, 200 nm to 2000 nm, or 500 nm to 1000 nm. Most preferably, the size of the
nano sized particles range from about 50 nm to about 200 nm.
[00121] In an embodiment of the present invention, the harsh chemical-free
self-disinfecting composition comprises nanoparticles of a herbal organic compound at a concentration of at least about 0.01% w/v, more usually at least about 0.015%) w/v, at least about 0.02% w/v, at least about 0.025%> w/v, at least about 0.03%) w/v, and not more than about 0.035%> w/v or about 0.04% w/v, but usually not more than about 0.045%) w/v, or not more than about 0.05% w/v. Most preferably, the percentage of nanoparticles of a herbal organic compound is 0.025%> w/v with respect to the overall composition.
[00122] In an embodiment of the present invention, the harsh chemical-free
self-disinfecting composition comprises curcumin at a concentration of at least about 0.01%) w/v, more usually at least about 0.015%> w/v, at least about 0.02% w/v, at least about 0.025%> w/v, at least about 0.03% w/v, and not more than about 0.035%) w/v or about 0.04% w/v, but usually not more than about 0.045%) w/v, or not more than about 0.05% w/v. Most preferably, the percentage of curcumin is 0.025%) w/v with respect to the overall composition.

[00123] In an embodiment of the present invention, the herbal organic nanoparticle is selected from extracts such as grapefruit seed extract, grapefruit peel extract, pomegranate seed extract, citrus extract, oregano extract, thyme extract, green tea extract, white tea extract, soy extract, fermented soy protein, aloe vera extract, and aloe vera juice and combinations thereof; or individual components such as resveratrol, thyme, oregano, curcumin, 1-citronellol, a-amylcinnamaldehyde, lyral, geraniol, farnesol, hydroxycitronellal, isoeugenol, eugenol, camphor, eucalyptol, linalool, citral, thymol, limonene and menthol, and the like. Most preferably curcumin.
[00124] In an embodiment of the present disclosure, the biopolymer is selected from but not limited to PLGA, Poly (methylmethacrylate-comethacrylic acid), polysorbates, 3-(trimethoxysilyl) propyl methacrylate (TPM), polycaprolactone, polystyrene, Polyhydroxybutyrate, poly(vinyl alcohol), polylactatic acid (PLA), and the like. More preferably PLA.
[00125] In another embodiment, the present invention provides a harsh chemical-free self-disinfecting composition comprising nanoparticles suspended in pharmaceutical grade water or double distilled water comprising non-ionic surfactant, wherein the nanoparticles comprise a synergistic combination of Ti02 nanoparticles, silver nanoparticles, and nanoparticles of a herbal organic compound.
[00126] In another embodiment, the present invention provides a harsh chemical-free self-disinfecting composition comprising nanoparticles suspended in pharmaceutical grade water or double distilled water comprising non-ionic surfactant, wherein the nanoparticles comprise a synergistic combination of metal nanoparticles and biopolymer encapsulated nanoparticles of a herbal organic compound.
[00127] In preferred embodiment, the present invention provides a harsh chemical-free self-disinfecting composition comprising nanoparticles suspended in pharmaceutical grade water or double distilled water comprising poloxamer, wherein the nanoparticles comprise a synergistic combination of Ti02 nanoparticles, silver nanoparticles, and PLA encapsulated curcumin nanoparticles.

[00128] In preferred embodiment, the present invention provides a lyophilized harsh chemical-free self-disinfecting composition comprising nanoparticles suspended in pharmaceutical grade water or double distilled water comprising poloxamer, wherein the nanoparticles comprise a synergistic combination of Ti02 nanoparticles, silver nanoparticles, and PLA encapsulated curcumin nanoparticles. [00129] In an embodiment of the present disclosure, the lyophilized harsh chemical-free self-disinfecting composition has a storage stability for more than one year.
[00130] In preferred embodiment, the present invention provides a method for preparing a harsh chemical-free self-disinfecting composition comprises the steps of:
a. preparing a solution of non-ionic surfactant in glass beaker using
pharmaceutical grade water on a magnetic stirrer;
b. preparing a solution of biopolymer in acetonitrile in a separate glass
vial;
c. adding herbal organic nanoparticle to biopolymer solution from step
b), followed by brief sonication;
d. adding the mixture from step c) to aqueous phase from step a) under
continuous overnight stirring using magnetic stirrer to evaporate the
organic solution;
e. adding metallic nanoparticles to final solution from step d), followed
by mixing with magnetic stirrer.
[00131] In an embodiment of the present disclosure, the non-ionic surfactant is selected from but not limited to poloxamers, Span 20 (Sorbitan monolaurate), Span 40 (Sorbitan monopalmitate), Span 60 (Sorbitan monostearate), Tween 20 (Polyoxyethylene sorbitan monolaurate), Tween 60 (Polyoxyethylene sorbitan monostearate), Tween 80 (Polyoxyethylene sorbitan monooleate), and Span 85 (Sorbitan trioleate). More preferably poloxamer.
[00132] In preferred embodiment, the present invention provides a method for preparing a harsh chemical-free self-disinfecting composition comprises the steps of:

a. preparing a solution of poloxamer in a glass beaker using
pharmaceutical grade water on a magnetic stirrer;
b. preparing a solution of polylactic acid (PLA) in acetonitrile in a
separate glass vial;
c. adding curcumin to PLA solution from step b), followed by brief
sonication for 15 seconds;
d. adding the mixture from step c) to aqueous phase from step a) under
continuous overnight stirring using magnetic stirrer to evaporate the
organic solution;
e. adding Ti02 or Ag-doped Ti02 nanoparticles to final solution from
step d), followed by mixing with magnetic stirrer.
[00133] In an embodiment, the prepared chemical-free self-disinfecting
composition is has a zeta potential of-20.2 mV.
[00134] In an embodiment, the harsh chemical-free self-disinfecting
composition is provided in the form of a spray, liquid, or gel. Most preferably, the harsh chemical-free self-disinfecting composition realized in accordance with embodiments of the present disclosure is prepared in sprayable liquid form. [00135] In preferred embodiment of the present disclosure, the composition can be used to sanitize or disinfect the surfaces using a variety of methods and tools, including spraying with a trigger sprayer, pump sprayer, aerosol, or with an onboard spray device.
[00136] In an embodiment, the present invention relates to a harsh chemical-free hybrid self-disinfecting composition which is useful as a harsh chemical-free surface sanitizer composition by medical care-givers. The term 'harsh chemical-free surface sanitizer composition' as used herein throughout the present disclosure denotes the disinfectant composition, which needs to be sprayed on any surface and to dry to form a coating for killing all microbes.
[00137] In an embodiment, the harsh chemical-free self-disinfecting composition is provided in the lyophilized powder form. Most preferably, the lyophilized powder can be prepared in sprayable liquid form by mixing with water.

[00138] In preferred embodiment of the present invention, the harsh chemical-free self-disinfecting composition can be used for hard surface selected from the group consisting of stone, cement, wood, ceramic, fibrous material, glass, metal, polymer, and the like.
[00139] In an embodiment of the present disclosure, the harsh chemical-free self-disinfecting composition can be used for all kinds of floors selected from sheet floors (e.g., vinyl flooring, linoleum or rubber sheeting), vinyl composite tiles, luxury vinyl tiles, vinyl asbestos tiles, rubber tiles, cork and synthetic sports floors, and non-resilient substrates such as concrete, stone, marble, wood, bamboo, ceramic tile, grout, Terrazzo and other poured or "dry shake" floors, epoxy, polyvinyl chloride (PVC), and methyl methacrylates (MMA), cement, linoleum, rubber, and the like.
[00140] In an embodiment of the present disclosure, the harsh chemical-free self-disinfecting composition can be used as a hard surface cleaner, a kitchen cleaner, a bathroom cleaner, a toilet bowl cleaner, a glass cleaner, a floor cleaner, a carpet cleaner, a biocidal cleaner, an all-purpose general cleaner, and the like. [00141] In another embodiment of the present disclosure, the "hard surface" as used herein are, for example and in some cases preferable, sanitizing or disinfecting of hard-surfaces typically found in and around residential dwellings like bathrooms, kitchens, basements and garages, e.g., floors, walls, tiles, windows, sinks, showers, shower plasticized curtains, wash basins, drains, dishes, fixtures, and fittings and the like made of different materials like fiberglass and other car materials, leather, ceramic, painted and un-painted wood or concrete, varnished or sealed, plaster, bricks, vinyl, no-wax vinyl, linoleum, marble, melamine, glass, any plastics, metals, chromed surfaces and the like.
[00142] In further embodiment of the present disclosure, the "hard surface" also includes household appliances including, but not limited to, washing machines, automatic dryers, refrigerators, freezers, ovens, microwave ovens, dishwashers, and the like.
[00143] In further embodiment of the present disclosure, "hard surface" also includes the surfaces associated with medical facilities, e.g., hospitals, clinics as

well as laboratories among other industrial and/or commercial settings including, but not limited to restaurants, full service and fast food, sports facilities and other facilities using janitorial staff, and those found in cleaning industrial parts such as automobile and airplane industry, attached thereto, on, in or the like. [00144] In an embodiment, the harsh chemical-free self-disinfecting composition of the present invention maybe used on the body part in any manner known to a person skilled in the art. EXAMPLES
[00145] The present disclosure is further explained in the form of following
examples. However, it is to be understood that the foregoing examples are merely illustrative and are not to be taken as limitations upon the scope of the invention. Various changes and modifications to the disclosed embodiments will be apparent to those skilled in the art. Such changes and modifications may be made without departing from the scope of the invention.
[00146] Example 1: PLA encapsulated Curcumin nanoparticles preparation:
The dialysis tubing was activated by keeping under running water for 4 hours. The tubing was then immersed in a beaker containing 100 mM sodium bicarbonate (NaHC03) and 10 mM EDTA-Na salt (pH 7.0) for 2 h at 60 °C. Mild agitation was given using a magnetic bead. The tubes were then washed several times in MilliQ water and stored in MilliQ water containing 0.02 % sodium azide as preservative under refrigeration.
Briefly, 150mg PLA dissolved in 10ml Acetone and kept inside a dialysis tubing (cut off range lOkDa). The tubing was suspended in a beaker with 100ml Milli Q .The assembly was kept on a magnetic stirrer for overnight. The organic phase diffuses out through the dialysis tube / membrane into the aqueous phase which decrease the interfacial tension between them. A suspension of nanoparticles is formed by the displacement of solvent inside the membrane followed by progressive aggregation of polymer due to the loss of solubility. The resulting NPs suspension (in 2ml Deionized water) was transferred in to Amicon filter (lOkDa) and centrifuged at 2500 rpm for 15 mins. The process was repeated 3 to 4 time to remove any solvent left in the suspension.

[00147] Example 2: Characterization of PLA encapsulated Curcumin
nanoparticles:
Dynamic Light Scattering (DLS) and Zeta potential (Q
Particle size and zeta potential of DOX loaded PHB72K-PEG4K NPs were analyzed using Dynamic Light Scattering instrument (Malvern, UK) as shown in FIG. 1 and 2. The sample were diluted 103 times in mili-Q than 1 ml of the diluted samples were transferred to plastic cuvette. A scan rate of 20 scans per samples was used to acquire size and zeta potential of NPs.
[00148] Example 3: Antimicrobial activity of Curcumin nanoparticles
(CUR NPs):
Antimicrobial activity of CUR NPs and free CUR was carried by tube dilution method. CUR NPs in a series of concentration. The tubes are than inoculated with approximately log 5.7 CFU/ml of the E. coli strain. After incubation at 35+2 °C, the optical density of test strain was measured in a spectrophotometer at 600nm in a time dependent manner ranging from 0 to 20 hrs. The suspensions were appropriately diluted. The dilutions were plated on Luria agar and finally incubated for 12hrs at 37 °C in an incubator.
The results of antimicrobial activity of free CUR and CUR NPs. Both free CUR and CUR NPS were irradiated with a LED (420 nm) for 2mins at each time point before carrying antimicrobial activity. Free CUR showed significant antimicrobial action up to two hours in comparison to CUR NPs. Antimicrobial action of free CUR has been widely reported. Although OD for free CUR indicated a higher antimicrobial action of free CUR in comparison to CUR NPs at 3.5hrs. However a slight higher antimicrobial action was observed for CUR NPs at 3.5 hrs as confirmed by CFU/ml at this point of time.
CUR NPs showed higher antimicrobial action against E.coli after 18hrs in comparison to free CUR and as confirmed from CFU count. A higher antimicrobial action of 41% for CUR NPs at 18hrs is due to sustained release of active CUR from biodegradable polylactic acid polymer used to encapsulate Curcumin. Biodegradable NPs shows sustained release of active compounds in comparison to

free active compounds thereby decreasing the frequency multiple usage or
requirement of the active compound.
[00149] Example 4: Antimicrobial activity of PLA encapsulated Curcumin
nanoparticles (CUR NPs):
100 uL of each microbial suspension (107-108 CFU/mL) were individually
transferred to 96-well flat-bottom microtiter plates. To each well 100 uL free CUR
or anionic or cationic CUR-NPs were added to each microbial suspension resulting
in a final concentration (parameter to be optimized).
The plate was then kept in the dark for 40 minutes (pre-irradiation time) and, after
this period, it was illuminated from above by LED light for 20 minutes,
corresponding to a fluence of 43.2 J/cm2 (C+L+ groups). The toxicity of the NPs
were evaluated by incubating each microbial suspension with anionic or cationic
NPs without CUR in the dark for 60 minutes. The effect of light alone was verified
by adding 100 uL of PBS to each microbial suspension, incubating it for 40 minutes
and illuminating it for 20 minutes (43.2 J/cm2, C-L+ group). The untreated control
group did not receive any PS nor light (C-L-). Afterwards, each sample was
submitted to tenfold serial dilutions that were spread on specific agar media for
each microorganism as described previously. All agar plates were incubated at 37°C
for 48 hours for colony quantification (CFU/mL).
[00150] Example 5: Antimicrobial activity of Titanium Dioxide (Ti02)
nanoparticles (Ti02 NPs):
Preparation of Ti02 NPs stock
Ti02 NPs were procured from nano research element having average particle size
of 20nm. A stock of 5mg/ml (w/v) of Ti02 NPs was prepared in ultrapure water
and dilutions were prepared ranging from 10 to 1000 times from the initial
concentration in a 15ml test tube.
Activation of Microbial strain for antimicrobial activity
A strain of E. coli was activated from glycerol stock in to Luria broth. The test strain
was propagated in Luria broth for 12 hrs at 37 °C at 200 rpm in an incubator cum
shaker.
Antimicrobial Activity

Antimicrobial activity of Ti02 NPs was carried by tube dilution methodl. Ti02 NPs in a series of concentration (as mentioned in para 1). The tubes are than inoculated with approximately log 5.7 CFU/ml of the E. coli strain. After incubation at 35+2 °C, the optical density of test strain was measured in a spectrophotometer at 600nm in a time dependent manner ranging from 0 to 24 hrs. The suspensions were appropriately diluted and are plated on non-selective medium like Luria agar. The plates were finally incubated for 12hrs at 37 °C in an incubator. The result of antimicrobial activity of Ti02 NPs. Ti02 NPs at a concentration of lOOug/ml showed 23% killing after 2.0 hrs and showed maximum antimicrobial activity with 60% killing of test strain after 3.5 hrs. The percent killing reduced to 38 after 22 hrs.
The colony forming unit (CFU/ml) at four time points i.e 0, 2, 3.5 and 22 hrs (labeled as in the inset as l&l', 2&2', 3 &3', 2&2' hrs) also showed maximum antimicrobial action at 3.5hrs further supported the antimicrobial action of Ti02 NPs against the test strain.
In a parallel study to observe the enhanced antimicrobial action of Ti02 NPs in the presence of light was also carried. However, no significant increase in antimicrobial action against the test strain was observed.
[00151] Example 6: Composition of the harsh chemical-free hybrid self-
disinfecting spray composition
Table 1: For a standard batch of 20 ml the quantity

Ingredients Amount Vendors
Polylactic Acid (40K) 0.1 gm Sigma Aldrich
F-127Pluronic 0.2 gm Sigma Aldrich
Curcumin powder 0.005 gm Himedia
Ti02 NPs 0.005 gm Nano Research Elements
Acetonitrile 5 ml Fisher Scientific
Silver nanoparticles 0.005 gm Nano Research Elements

Mili-Q (De ionized water) q.s to make 20% w/v -
Table 2: For a standard batch of 100 ml the quantity

Ingredients Amount Vendors
Polylactic Acid (40K) 0.5 gm Sigma Aldrich
F-127Pluronic 1 gm Sigma Aldrich
Curcumin powder 0.025 gm Himedia
Ti02 NPs 0.025 gm Nano Research Elements
Acetonitrile 25 ml Fisher Scientific
Silver nanoparticles 0.025 gm Nano Research Elements
Mili-Q (De ionized water) q.s to make 100% w/v -
[00152] Example 7: Preparation of the harsh chemical-free hybrid self-
disinfecting spray composition
Solution A: 20ml DI water was added in a small beaker and then 200mg F127 mixed at room temperature and stirred with magnetic bead until completely mixed. Solution B: In a vial for solution B, 6 ml acetonitrile added, followed by a 35mg PLA bead. The vial was kept at 40-55°C and stirred using a magnetic stirrer with 1200rpm without using a magnetic bead to allow the PLA bead to dissolve completely.
3.5mg curcumin was added in the vial (solution B) and stirred properly. An injection syringe was filled with 6ml of the solution B.
Solution B was added into Solution A, drop wise with continuous stirring for 24 hours while keeping the solution covered properly in dark till the organic solvent was evaporated completely to form a beaker solution of around 19-20 ml. 3.5 mg titanium dioxide and 3.5 mg silver nanoparticles were added and kept on stirrer for 30 minutes to one hour, followed by lyophilization to make longer shelf-life but with same potency.
[00153] Example 8: In-vitro Antimicrobial Activity of the harsh chemical-
free hybrid self-disinfecting spray composition

Mixed culture of Staphylococcus aureus, Bacillus cereus, Pseudomonas aeruginosa, Eschericla coli and Salmonella was used to check the Antibacterial Efficacy of composition from Example 7 at different time intervals and different surfaces. Swab test- Swabs are plated out and incubated at 37°C for 72 hrs. Colonies are enumerated after incubation and results are calculated in percentage by reduction
of bacterial load. (FIG. 4A-4D)
Table 3: Marble

s.
No. Test Parameters Unit of Measurement Time of contact (Post-Treatment) Results Method Reference
1 Antibacterial Efficacy % 15 min 99.99 In-House Method
2 Antibacterial Efficacy % 24 Hrs 99.85 In-House Method
3 Antibacterial Efficacy % 48 Hrs 99.21 In-House Method
4 Antibacterial Efficacy % 72 Hrs 95.05 In-House Method
5 Antibacterial Efficacy % 96 Hrs 90.07 In-House Method
Table 4: Metal

s.
No. Test Parameters Unit of Measurement Time of contact (Post-Treatment) Results Method Reference
1 Antibacterial Efficacy % 15 min 99.99 In-House Method
2 Antibacterial Efficacy % 24 Hrs 99.90 In-House Method
3 Antibacterial Efficacy % 48 Hrs 99.87 In-House Method
4 Antibacterial Efficacy % 72 Hrs 95.21 In-House Method
5 Antibacterial Efficacy % 96 Hrs 95.06 In-House Method

Table 5: Wood

s.
No. Test Parameters Unit of Measurement Time of contact (Post-Treatment) Results Method Reference
1 Antibacterial Efficacy % 15 min 99.99 In-House Method
2 Antibacterial Efficacy % 24Hrs 99.67 In-House Method
3 Antibacterial Efficacy % 48Hrs 99.60 In-House Method
4 Antibacterial Efficacy % 72Hrs 95.09 In-House Method
5 Antibacterial Efficacy % 96Hrs 90.38 In-House Method
[00154] Example 9: Skin irritation testing of the harsh chemical-free self-
disinfecting composition:
The harsh chemical-free self-disinfecting composition from Example 1 is made into 150 ml pockets each and anonymously handed over to 5 volunteers to test the adverse effects or skin irritancy of the composition. Each of the subjects were instructed to use 5 ml of formulated spray on an area of 2 square inch to the back of subjects' hands continuously for 30 days. The subjects were observed for heat, swelling, rashes, redness and irritation. None of the subjects reported of adverse effects or irritation while using or after using the composition. The acceptability study on 5 human volunteers showed that the harsh chemical-free self-disinfecting composition did not produce any signs of skin irritation.
Table 6: Volunteer Responses

S.No Response (Feedback) No. of Volunteers (5)
1. Skin irritancy No Irritation
2. Rashes No Rashes
3. Redness No Redness
4. Swelling No Swelling

5. Heat No Heat
[00155] While the foregoing describes various embodiments of the disclosure, other and further embodiments of the disclosure may be devised without departing from the basic scope thereof. The scope of the invention is determined by the claims that follow. The invention is not limited to the described embodiments, versions or examples, which are included to enable a person having ordinary skill in the art to make and use the invention when combined with information and knowledge available to the person having ordinary skill in the art.

We Claim:
1. A self-disinfecting composition comprising nanoparticles suspended in
pharmaceutical grade water or double distilled water comprising non-ionic
surfactant for disinfecting or sanitizing surfaces, wherein the nanoparticles
comprise
a. a synergistic combination of metallic nanoparticles and biopolymer encapsulated nanoparticles of a herbal organic compound; characterized in that:
- the composition forms a disinfectant layer on the surface and is effective even after 96 hrs after application;
- the composition is environment-friendly and biodegradable;
- the composition does not comprise alcohol, hypochlorite, acid, or toxic chemicals; and
- the composition has no side effect on eye and skin.

2. The composition as claimed in claim 1, wherein the composition is a lyophilized composition comprising a storage stability of at least one year.
3. The composition as claimed in claim 1, wherein the metal nanoparticle is Ti02 nanoparticle or silver nanoparticle or combinations thereof, each in an amount ranging from about 0.001 to about 0.025% w/v of the total composition.
4. The composition as claimed in claim 1, wherein the herbal compound is curcumin in an amount ranging from about 0.001 to about 0.025% w/v of the total composition.
5. The composition as claimed in claim 1, wherein the biopolymer is polycaprolactone or polylactic acid (PLA) in an amount ranging from about 0.1 to about 0.5%) w/v of the total composition.

6. The composition as claimed in claim 5, wherein polycaprolactone or polylactic acid (PLA) is dissolved in acetonitrile in an amount ranging from about 10 to about 25% w/v of the total composition.
7. The composition as claimed in claim 1, wherein the non-ionic surfactant is poloxamer in an amount ranging from about 0.1 to about 1.0% w/v of the total composition.
8. A self-disinfecting composition comprising nanoparticles suspended in pharmaceutical grade water or double distilled water comprising poloxamer for disinfecting or sanitizing surfaces, wherein the nanoparticles comprise
a. a synergistic combination of Ti02 nanoparticles, silver nanoparticles and PLA encapsulated curcumin nanoparticles; characterized in that:
- the composition forms a disinfectant layer on the surface and is effective even after 96 hrs after application;
- the composition is environment-friendly and biodegradable;
- the composition does not comprise alcohol, hypochlorite, acid, or toxic chemicals; and
- the composition has no side effect on eye and skin.
9. A method for preparing a harsh chemical-free self-disinfecting composition
comprises the steps of:
a. preparing a solution of poloxamer in glass beaker using
pharmaceutical grade water on a magnetic stirrer;
b. preparing a solution of polylactic acid (PLA) in acetonitrile in a
separate glass vial;
c. adding curcumin to PLA solution from step b), followed by brief
sonication for 15 seconds;

d. adding the mixture from step c) to aqueous phase from step a) under
continuous overnight stirring using magnetic stirrer to evaporate the
organic solution;
e. adding Ti02 nanoparticles to final solution from step d), followed
by mixing with magnetic stirrer.
10. A disinfecting composition comprising the composition as claimed in any one of claim 1, 2 or 8.