TECHNICAL FIELD
[001] The subject matter described herein in general relates to the field of skin-beneficial cosmetics, and in particular relates to skin anti-aging compositions.
BACKGROUND OF INVENTION
[002] Skin aging is influenced by several factors, such as, exposure to ultraviolet radiation, environmental pollution, alcohol and tobacco abuse, heredity, lifestyle, body weight, etc., to name a few. Skin aging is associated with degradation of extracellular matrix (ECM), decreased rate of cellular renewal, loss of cellular hydration due to impaired skin barrier function, and loss of subcutaneous fat, all of which result in appearance of fine lines and wrinkles on the skin, which is one the hallmarks of aging. In the recent years, collagen and α-smooth muscle actin (SMA) have been a target of investigation to reduce/ slow down aging. [003] Numerous literature is directed to improve collagen production in mammals through use of plant extracts. For example, WO2014115137 discloses a cosmetic skin anti-aging composition comprising niacinamide or derivative thereof, coenzyme Q10 or derivative thereof, a composition of caprylic/capric triglyceride and geranylgeranylpropanol, Lepidium sativum sprout extract and gold. FR2934779A1 describes the use of at least one extract from Ceratonia siliqua as an active ingredient in a cosmetic composition for skin tissue repair. WO2016133775A1 discloses a topical composition and a method for treating skin to boost collagen production are provided. The composition comprises a collagen-boosting effective amount of a mixture of acetyl hexapeptide-3, Siegesbeckia orientalis extract, Laminaria digitata, whey protein, and magnesium ascorbyl phosphate.
[004] Although a lot of research has been done in the past to develop effective skin compositions, there still exists a need to develop compositions that could enhance the inherent ability of fibroblast cells to synthesis collagen, and also upregulate α-SMA, to retain the tensile strength of skin and thereby delay/reverse the formation of wrinkles.

SUMMARY OF THE INVENTION
[005] In an aspect of the present invention, there is provided a skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan; b) 6-furfurylaminopurine; c) Cyprus rotundus root extract (and) glycerine (and) water; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; e) niacinamide; f) sodium hyaluronate; and g) african pepper extract, wherein the composition is transparent. [006] In another aspect of the present invention, there is provided a process for preparing the skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan; b) 6-furfurylaminopurine; c) Cyprus rotundus root extract (and) glycerine (and) water; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; e) niacinamide; f) sodium hyaluronate; and g) african pepper extract, wherein the composition is transparent; said process comprising: i) obtaining aqua (and) glycerine (and) beta-glucan; ii) obtaining 6-furfurylaminopurine; iii) obtaining Cyprus rotundus root extract (and) glycerine (and) water; iv) obtaining Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; v) obtaining niacinamide; vi) obtaining sodium hyaluronate; vii) obtaining african pepper extract; and viii) contacting aqua (and) glycerine (and) beta-glucan, 6-furfurylaminopurine, Cyprus rotundus root extract (and) glycerine (and) water, Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water, niacinamide, sodium hyaluronate, and african pepper extract, to obtain the composition.
[007] These and other features, aspects, and advantages of the present subject matter will be better understood with reference to the following description and appended claims. This summary is provided to introduce a selection of concepts in a simplified form. This summary is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used to limit the scope of the claimed subject matter.
BRIEF DESCRIPTION OF THE DRAWINGS

[008] The detailed description is described with reference to the accompanying
figures. The same numbers are used throughout the drawings to reference like
features and components.
[009] Figure 1 depicts the effect of the compositions SAA-HCC, SAA-P, retinoic
acid (RA), and control, on the effect of collagen protein production, in accordance
with an embodiment of the present subject matter.
[0010] Figure 2 depicts a graph comparing a fold change in collagen gene
expression in human dermal fibroblasts (HDF) upon treatment with the
compositions SAA-HCC, SAA-P, RA, and control, in accordance with an
embodiment of the present subject matter.
[0011] Figure 3 depicts the graph comparing a fold change in alpha smooth muscle
actin (SMA) expression in human dermal fibroblasts (HDF) upon treatment with
the compositions SAA-HCC, SAA-P, RA, and control, in accordance with an
embodiment of the present subject matter.
[0012] Figure 4a depicts the effect of time on the colour change of the composition
at time (T) = 0, in accordance with an embodiment of the present subject matter.
[0013] Figure 4b depicts the effect of time on the colour change of the composition
at time (T) = 24h, in accordance with an embodiment of the present subject matter.
[0014] Figure 4c depicts the effect of time on the colour change of the composition
at time (T) = 120h, in accordance with an embodiment of the present subject
matter.
DETAILED DESCRIPTION OF THE INVENTION
[0015] Those skilled in the art will be aware that the present disclosure is subject to
variations and modifications other than those specifically described. It is to be
understood that the present disclosure includes all such variations and
modifications. The disclosure also includes all such steps, features, compositions,
and compounds referred to or indicated in this specification, individually or
collectively, and all combinations of any or more of such steps or features.
Definitions
[0016] For convenience, before further description of the present disclosure,
certain terms employed in the specification, and examples are delineated here.

These definitions should be read in the light of the remainder of the disclosure and
understood as by a person of skill in the art. The terms used herein have the
meanings recognized and known to those of skill in the art, however, for
convenience and completeness, particular terms and their meanings are set forth
below.
[0017] The articles “a”, “an” and “the” are used to refer to one or to more than one
(i.e., to at least one) of the grammatical object of the article.
[0018] The terms “comprise” and “comprising” are used in the inclusive, open
sense, meaning that additional elements may be included. It is not intended to be
construed as “consists of only”.
[0019] Throughout this specification, unless the context requires otherwise the
word “comprise”, and variations such as “comprises” and “comprising”, will be
understood to imply the inclusion of a stated element or step or group of element or
steps but not the exclusion of any other element or step or group of element or
steps.
[0020] The term “including” is used to mean “including but not limited to”.
“Including” and “including but not limited to” are used interchangeably.
[0021] Ratios, concentrations, amounts, and other numerical data may be presented
herein in a range format. It is to be understood that such range format is used
merely for convenience and brevity and should be interpreted flexibly to include
not only the numerical values explicitly recited as the limits of the range, but also
to include all the individual numerical values or sub-ranges encompassed within
that range as if each numerical value and sub-range is explicitly recited.
[0022] The term “at least one” is used to mean one or more and thus includes
individual components as well as mixtures/combinations.
[0023] For the purposes of the present disclosure, the term “transparent” refers to
allowing light to pass through so that objects behind can be distinctly seen.
[0024] The term “anti-aging” refers to compositions designed to prevent the
appearance of getting older.
[0025] The term “skin-beneficial” refers to compositions that are beneficial or
improve the appearance or lustre to the skin.

[0026] For the purposes of the present disclosure, the terms “emollients”, “humectants”, “preservatives”, “silicones”, “chelators”, “moisturization agents”, and “sunscreen agents” refer to the generally known components used in the art. The specific components of the above-described categories have been mentioned in the present disclosure wherever applicable. Wherever not mentioned, it can be construed that any component falling under the categories can be used as part of the composition.
[0027] Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs. Although any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the disclosure, the preferred methods, and materials are now described. All publications mentioned herein are incorporated herein by reference.
[0028] The present disclosure is not to be limited in scope by the specific embodiments described herein, which are intended for the purposes of exemplification only. Functionally-equivalent products, compositions, and methods are clearly within the scope of the disclosure, as described herein.
[0029] Conventionally various treatments which involve the use of vitamin A-derived substances, such as topical retinoic acid or retin-A; natural products, such as alpha hydroxy acids and anti- oxidants; exposure to light treatments, such as near infra-red (NIR) light; and more aggressive treatments such as dermabrasion, injections with botulinum toxin, chemical peels, laser resurfacing, face lifts, and so forth have been adopted for persons showing signs of aging (WO201613377). However, most of these treatments have been associated with significant side effects. Therefore, there still exists a need to develop novel compositions that overcome the drawbacks associated with prior art. Although numerous compositions, derived from plant sources, have been described in the past, there still is a continuing interest in providing novel skin beneficial compositions which demonstrate efficacy in preventing, slowing and /or reversing the visible signs of aging on the skin. Accordingly, the present disclosure provides for a transparent skin beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan;

b) 6-furfurylaminopurine; c) Cyprus rotundus root extract (and) glycerine (and) water; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; e) niacinamide; f) sodium hyaluronate; and g) african pepper extract. The compositions of the present disclosure can be effectively used in the cosmetic industry to combat skin-aging. [0030] African Pepper (Afromomum Melegueta) belongs to the Zingiberacea family. It is a spice growing around the Guinea gulf with a zesty flavour reminiscent of ginger and cardamom. During the 15th century, these rare seeds were hightly prized as an exotic flavour by Europeans, who used these “grains of paradise” in food and beverages. It was also believed to act as an aphrodisiac. In Africa, the seeds were traditionally chewed as sedatives against toothache and to warm the body. Its active spectrum contains 6-gingerol, 6-paradol and 6-shoagol, the spicy substances of the ginger family which perform energising properties. Its essential oil, rich in humulene and caryophyllene, supports the antibacterial effect of the spice. The 6-ginegerol content in in the range of 1200 – 5000 ppm. [0031] In an embodiment of the present disclosure, there is provided a skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan; b) 6-furfurylaminopurine; c) Cyprus rotundus root extract (and) glycerine (and) water; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; e) niacinamide; f) sodium hyaluronate; and g) african pepper extract, wherein the composition is transparent. [0032] In an embodiment of the present disclosure, there is provided a skin-beneficial composition as described herein, wherein aqua (and) glycerine (and) beta-glucan has a weight percentage in a range of 1-5% with respect to the composition, 6-furfurylaminopurine having a weight percentage in a range of 0.01-0.1% with respect to the composition, Cyprus rotundus root extract (and) glycerine (and) water has a weight percentage in a range of 0.1-2% with respect to the composition, Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water has a weight percentage in a range of 0.05-1% with respect to the composition, niacinamide has a weight percentage in a range of 0.05-5% with respect to the composition, sodium

hyaluronate has a weight percentage in a range of 0.01-0.5% with respect to the composition, african pepper extract has a weight percentage in a range of 0.01-3% with respect to the composition. In another embodiment of the present disclosure, the aqua (and) glycerine (and) beta-glucan has a weight percentage in a range of 1-2.5% with respect to the composition, 6-furfurylaminopurine having a weight percentage in a range of 0.01-0.03% with respect to the composition, Cyprus rotundus root extract (and) glycerine (and) water has a weight percentage in a range of 0.1-0.5% with respect to the composition, Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water has a weight percentage in a range of 0.05-0.1% with respect to the composition, niacinamide has a weight percentage in a range of 0.05-2% with respect to the composition, sodium hyaluronate has a weight percentage in a range of 0.01-0.05% with respect to the composition, african pepper extract has a weight percentage in a range of 0.01-1% with respect to the composition. [0033] In an embodiment of the present disclosure, there is provided a skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan having a weight percentage in a rage of 1-5% with respect to the composition; b) 6-furfurylaminopurine having a weight percentage in a range of 0.01-0.1% with respect to the composition; c) Cyprus rotundus root extract (and) glycerine (and) water having a weight percentage in a range of 0.1-2% with respect to the composition; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water having a weight percentage in a range of 0.05-1% with respect to the composition; e) niacinamide having a weight percentage in a range of 0.05-5% with respect to the composition; f) sodium hyaluronate having a weight percentage in a range of 0.01-0.5% with respect to the composition; and g) african pepper extract having a weight percentage in a range of 0.01-3% with respect to the composition, wherein the composition is transparent.
[0034] In an embodiment of the present disclosure, there is provided a skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan having a weight percentage in a range of 1-2.5% with respect to the composition;

b) 6-furfurylaminopurine having a weight percentage in a range of 0.01-0.03% with respect to the composition; c) Cyprus rotundus root extract (and) glycerine (and) water having a weight percentage in a range of 0.1-0.5% with respect to the composition; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water having a weight percentage in a range of 0.05-0.1% with respect to the composition; e) niacinamide having a weight percentage in a range of 0.05-2% with respect to the composition; f) sodium hyaluronate having a weight percentage in a range of 0.01-0.05% with respect to the composition; and g) african pepper extract having a weight percentage in a range of 0.01-1% with respect to the composition, wherein the composition is transparent.
[0035] In an embodiment of the present disclosure, there is provided a skin-beneficial composition as described herein, wherein the composition further comprises at least one excipient selected from a group consisting of emollient, humectants, polymers, silicones, preservatives, fragrance, vitamins, chelators, sunscreen agents, moisturization agents, and combinations thereof. In an embodiment, the humectant is glycerin; the silicones include dimethicone; the preservative is phenoxyethanol; the vitamin is tocopherol acetate; the chelator is disodium ethylenediaminetetraacetic (EDTA); the sunscreen agent is ethylhexyl methoxycinnamate; and the moisturization agent is glycerin.
[0036] In an embodiment of the present disclosure, there is provided a skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan; b) 6-furfurylaminopurine; c) Cyprus rotundus root extract (and) glycerine (and) water; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; e) niacinamide; f) sodium hyaluronate; g) african pepper extract, and h) at least one excipient selected from a group consisting of emollient, humectants, polymers, silicones, preservatives, fragrance, vitamins, chelators, sunscreen agents, moisturization agents, and combinations thereof. In another embodiment of the present disclosure, the humectant is glycerin; the silicones include dimethicone; the preservative is phenoxyethanol; the vitamin is tocopherol acetate; the chelator is disodium

ethylenediaminetetraacetic (EDTA); the sunscreen agent is ethylhexyl methoxycinnamate; and the moisturization agent is glycerin.
[0037] In an embodiment of the present disclosure, there is provided a process for preparing the skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan; b) 6-furfurylaminopurine; c) Cyprus rotundus root extract (and) glycerine (and) water; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; e) niacinamide; f) sodium hyaluronate; and g) african pepper extract, wherein the composition is transparent; said process comprising: i) obtaining aqua (and) glycerine (and) beta-glucan; ii) obtaining 6-furfurylaminopurine; iii) obtaining Cyprus rotundus root extract (and) glycerine (and) water; iv) obtaining Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; v) obtaining niacinamide; vi) obtaining sodium hyaluronate; vii) obtaining african pepper extract; and viii) contacting aqua (and) glycerine (and) beta-glucan, 6-furfurylaminopurine, Cyprus rotundus root extract (and) glycerine (and) water, Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water, niacinamide, sodium hyaluronate, and african pepper extract, to obtain the composition.
[0038] In an embodiment of the present disclosure, there is provided a process for preparing the skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan having a weight percentage in a rage of 1-5% with respect to the composition; b) 6-furfurylaminopurine having a weight percentage in a range of 0.01-0.1% with respect to the composition; c) Cyprus rotundus root extract (and) glycerine (and) water having a weight percentage in a range of 0.1-2% with respect to the composition; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water having a weight percentage in a range of 0.05-1% with respect to the composition; e) niacinamide having a weight percentage in a range of 0.05-5% with respect to the composition; f) sodium hyaluronate having a weight percentage in a range of 0.01-0.5% with

respect to the composition; and g) african pepper extract having a weight percentage in a range of 0.01-3% with respect to the composition, wherein the composition is transparent; said process comprising: i) obtaining aqua (and) glycerine (and) beta-glucan; ii) obtaining 6-furfurylaminopurine; iii) obtaining Cyprus rotundus root extract (and) glycerine (and) water; iv) obtaining Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; v) obtaining niacinamide; vi) obtaining sodium hyaluronate; vii) obtaining african pepper extract; and viii) contacting aqua (and) glycerine (and) beta-glucan, 6-furfurylaminopurine, Cyprus rotundus root extract (and) glycerine (and) water, Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water, niacinamide, sodium hyaluronate, and african pepper extract, to obtain the composition.
[0039] In an embodiment of the present disclosure, there is provided a process for preparing the skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan having a weight percentage in a range of 1-2.5% with respect to the composition; b) 6-furfurylaminopurine having a weight percentage in a range of 0.01-0.03% with respect to the composition; c) Cyprus rotundus root extract (and) glycerine (and) water having a weight percentage in a range of 0.1-0.5% with respect to the composition; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water having a weight percentage in a range of 0.05-0.1% with respect to the composition; e) niacinamide having a weight percentage in a range of 0.05-2% with respect to the composition; f) sodium hyaluronate having a weight percentage in a range of 0.01-0.05% with respect to the composition; and g) african pepper extract having a weight percentage in a range of 0.01-1% with respect to the composition, wherein the composition is transparent; said process comprising: i) obtaining aqua (and) glycerine (and) beta-glucan; ii) obtaining 6-furfurylaminopurine; iii) obtaining Cyprus rotundus root extract (and) glycerine (and) water; iv) obtaining Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; v) obtaining

niacinamide; vi) obtaining sodium hyaluronate; vii) obtaining african pepper extract; and viii) contacting aqua (and) glycerine (and) beta-glucan, 6-furfurylaminopurine, Cyprus rotundus root extract (and) glycerine (and) water, Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water, niacinamide, sodium hyaluronate, and african pepper extract, to obtain the composition.
[0040] In an embodiment of the present disclosure, there is provided a process for preparing the skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan; b) 6-furfurylaminopurine; c) Cyprus rotundus root extract (and) glycerine (and) water; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; e) niacinamide; f) sodium hyaluronate; g) african pepper extract, and h) at least one excipient selected from a group consisting of emollient, humectants, polymers, silicones, preservatives, fragrance, vitamins, chelators, sunscreen agents, moisturization agents, and combinations thereof; wherein the composition is transparent; said process comprising: i) obtaining aqua (and) glycerine (and) beta-glucan; ii) obtaining 6-furfurylaminopurine; iii) obtaining Cyprus rotundus root extract (and) glycerine (and) water; iv) obtaining Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; v) obtaining niacinamide; vi) obtaining sodium hyaluronate; vii) obtaining african pepper extract; viii) obtaining at least one excipient; and ix) contacting aqua (and) glycerine (and) beta-glucan, 6-furfurylaminopurine, Cyprus rotundus root extract (and) glycerine (and) water, Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water, niacinamide, sodium hyaluronate, african pepper extract, and at least one excipient to obtain the composition. [0041] In an embodiment of the present disclosure, there is provided a skin-beneficial composition as described herein, wherein the composition increases α-smooth muscle actin (α-SMA) gene expression in a range of 2-5 folds. In another embodiment, the composition increases α-SMA gene expression in a range of 3-4 folds.

[0042] In an embodiment of the present disclosure, there is provided a skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan; b) 6-furfurylaminopurine; c) Cyprus rotundus root extract (and) glycerine (and) water; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; e) niacinamide; f) sodium hyaluronate; and g) african pepper extract, wherein the composition is transparent; and wherein the composition increases α-smooth muscle actin (α-SMA) gene expression in a range of 2-5 folds.
[0043] In an embodiment of the present disclosure, there is provided a skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan having a weight percentage in a rage of 1-5% with respect to the composition; b) 6-furfurylaminopurine having a weight percentage in a range of 0.01-0.1% with respect to the composition; c) Cyprus rotundus root extract (and) glycerine (and) water having a weight percentage in a range of 0.1-2% with respect to the composition; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water having a weight percentage in a range of 0.05-1% with respect to the composition; e) niacinamide having a weight percentage in a range of 0.05-5% with respect to the composition; f) sodium hyaluronate having a weight percentage in a range of 0.01-0.5% with respect to the composition; and g) african pepper extract having a weight percentage in a range of 0.01-3% with respect to the composition, wherein the composition is transparent; and wherein the composition increases α-smooth muscle actin (α-SMA) gene expression in a range of 2-5 folds. [0044] In an embodiment of the present disclosure, there is provided a skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan; b) 6-furfurylaminopurine; c) Cyprus rotundus root extract (and) glycerine (and) water; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; e) niacinamide; f) sodium hyaluronate; g) african pepper extract, and h) at least one excipient selected from a group consisting of emollient, humectants, polymers, silicones, preservatives, fragrance, vitamins, chelators, sunscreen agents, moisturization agents, and

combinations thereof, wherein the composition is transparent; and wherein the composition increases α-smooth muscle actin (α-SMA) gene expression in a range of 2-5 folds.
[0045] In an embodiment of the present disclosure, there is provided a skin-beneficial composition as described herein, wherein the composition increases collagen gene expression in a range of 4-6 folds. In another embodiment of the present disclosure, the composition increases collagen gene expression in the range of 4-5 folds.
[0046] In an embodiment of the present disclosure, there is provided a skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan; b) 6-furfurylaminopurine; c) Cyprus rotundus root extract (and) glycerine (and) water; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; e) niacinamide; f) sodium hyaluronate; and g) african pepper extract, wherein the composition is transparent; and wherein the composition increases collagen gene expression in a range of 4-6 folds.
[0047] In an embodiment of the present disclosure, there is provided a skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan; b) 6-furfurylaminopurine; c) Cyprus rotundus root extract (and) glycerine (and) water; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; e) niacinamide; f) sodium hyaluronate; and g) african pepper extract, wherein the composition is transparent, and wherein the composition increases α-smooth muscle actin (α-SMA) gene expression in a range of 2-5 folds, and wherein the composition increases collagen gene expression in a range of 4-6 folds.
[0048] In an embodiment of the present disclosure, there is provided a skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan having a weight percentage in a rage of 1-5% with respect to the composition; b) 6-furfurylaminopurine having a weight percentage in a range of 0.01-0.1% with respect to the composition; c) Cyprus rotundus root extract (and) glycerine (and) water having a weight percentage in a range of 0.1-2% with respect to the

composition; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water having a weight percentage in a range of 0.05-1% with respect to the composition; e) niacinamide having a weight percentage in a range of 0.05-5% with respect to the composition; f) sodium hyaluronate having a weight percentage in a range of 0.01-0.5% with respect to the composition; and g) african pepper extract having a weight percentage in a range of 0.01-3% with respect to the composition, wherein the composition is transparent, and wherein the composition increases collagen gene expression in a range of 4-6 folds.
[0049] In an embodiment of the present disclosure, there is provided a skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan having a weight percentage in a rage of 1-5% with respect to the composition; b) 6-furfurylaminopurine having a weight percentage in a range of 0.01-0.1% with respect to the composition; c) Cyprus rotundus root extract (and) glycerine (and) water having a weight percentage in a range of 0.1-2% with respect to the composition; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water having a weight percentage in a range of 0.05-1% with respect to the composition; e) niacinamide having a weight percentage in a range of 0.05-5% with respect to the composition; f) sodium hyaluronate having a weight percentage in a range of 0.01-0.5% with respect to the composition; and g) african pepper extract having a weight percentage in a range of 0.01-3% with respect to the composition, wherein the composition is transparent, and wherein the composition increases α-smooth muscle actin (α-SMA) gene expression in a range of 2-5 folds; and wherein the composition increases collagen gene expression in a range of 4-6 folds. [0050] In an embodiment of the present disclosure, there is provided a skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan; b) 6-furfurylaminopurine; c) Cyprus rotundus root extract (and) glycerine (and) water; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; e) niacinamide; f) sodium hyaluronate; g) african pepper extract, and h) at least one excipient selected from a

group consisting of emollient, humectants, polymers, silicones, preservatives, fragrance, vitamins, chelators, sunscreen agents, moisturization agents, and combinations thereof, wherein the composition is transparent, and wherein the composition increases collagen gene expression in a range of 4-6 folds. [0051] In an embodiment of the present disclosure, there is provided a skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan; b) 6-furfurylaminopurine; c) Cyprus rotundus root extract (and) glycerine (and) water; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; e) niacinamide; f) sodium hyaluronate; g) african pepper extract, and h) at least one excipient selected from a group consisting of emollient, humectants, polymers, silicones, preservatives, fragrance, vitamins, chelators, sunscreen agents, moisturization agents, and combinations thereof, wherein the composition is transparent, and wherein the composition increases α-smooth muscle actin (α-SMA) gene expression in a range of 2-5 folds, and wherein the composition increases collagen gene expression in a range of 4-6 folds.
[0052] In an embodiment of the present disclosure, there is provided a skin-beneficial composition as described herein, wherein the composition is an anti-ageing composition.
[0053] In an embodiment of the present disclosure, there is provided a skin-beneficial composition as described herein, wherein the composition is used in the form of ointments, creams, lotions, or any other unit dosage forms such as tablets, capsules, sachets, and the like.
[0054] Although the subject matter has been described with reference to specific embodiments, this description is not meant to be construed in a limiting sense. Various modifications of the disclosed embodiments, as well as alternate embodiments of the subject matter, will become apparent to persons skilled in the art upon reference to the description of the subject matter. It is therefore contemplated that such modifications can be made without departing from the spirit or scope of the present subject matter as defined.
EXAMPLES

[0055] The disclosure will now be illustrated with working examples, which is intended to illustrate the working of disclosure and not intended to take restrictively to imply any limitations on the scope of the present disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods and compositions, the exemplary methods, devices and materials are described herein. It is to be understood that this disclosure is not limited to particular methods, and experimental conditions described, as such methods and conditions may apply. [0056] The working examples described herein clearly depict the effect of the composition of the present disclosure on collagen α-smooth muscle actin (α-SMA) gene expression. The present disclosure is directed to a relative colourless aqueous concoction of actives for skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan; b) 6-furfurylaminopurine; c) Cyprus rotundus root extract (and) glycerine (and) water; d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; e) niacinamide; f) sodium hyaluronate; and g) african pepper extract.
Composition
[0057] Two compositions, namely test composition ‘SAA-HCC 3’, and composition of the present disclosure ‘SAA-P’ were prepared in which all the constituents, were combined in different fractions, as provided below in Table 1. [0058] The component Cyprus rotundus root extract (and) glycerine (and) water was commercially procured (Actipone® Nutgrass (Motha) Root GW). The component Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water was commercially procured (PhytoCellTecTM Malus Domestica). The component Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water is a cosmetic active ingredient containing apple stem cell compounds encapsulated in liposomes prepared from soy phospholipids. African pepper extract was commercially procured, and all the other components

used for preparing the composition of the present disclosure were procured commercially. African pepper extract comprises 6-gingerol, shogaol, paradol, and essential oil rich in humulene and carophyllene as the actives. The African pepper extract used in the present disclosure is a seed extract along with butylene alcohol as a carrier. The African pepper extract used in the present disclosure is characterized by 6-gingerol having a concentration in a range of 1200 ppm to 5000 ppm. T

[0059] The Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water comprises weight percentages as listed in Table 2. Table 2


Process for preparation of the composition
[0060] The compositions as provided in Table 1 was prepared by obtaining aqua (and) glycerine (and) beta-glucan; obtaining 6-furfurylaminopurine; obtaining Cyprus rotundus root extract (and) glycerine (and) water; obtaining Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; obtaining niacinamide; obtaining sodium hyaluronate; obtaining african pepper extract; optionally obtaining L-histidine, L-carnosine, and L-citrulline, and contacting aqua (and) glycerine (and) beta-glucan, 6-furfurylaminopurine, Cyprus rotundus root extract (and) glycerine (and) water, Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water, niacinamide, sodium hyaluronate, african pepper extract, and optionally L-histidine, L-carnosine, and L-citrulline, to obtain the composition. This is a general process for preparing the above-mentioned compositions. As per the requirements, the respective components were added to arrive at the composition. The mixing was done to achieve a homogenous composition, and the mixing was done at room temperature (25-30℃).
MATERIALS AND METHODS
Sircol collagen assay
[0061] Human dermal fibroblasts (HDF) were seeded in 12 well plates (from Costar) in Dulbecco's Modified Eagle Medium (DMEM) high glucose containing 2% fetal bovine serum (Gibco). The culture was incubated in 5% CO2 incubator

and was allowed to reach confluency, to form a fibroblast bed. HaCaT cells at a seeding density of 0.11 million cells per well were seeded on the fibroblast bed. Keratinocyte basal medium with supplements (Gibco) containing 0.03mM calcium chloride was used to seed the HaCaTs and the culture was maintained for 24 hrs for cell attachment and acclimatization. Post 24 hrs, treatments were initiated. After 48hrs of treatment, spent medium was harvested to determine total collagen based on the manufacturer’s protocol (Biocolor life science assays). Gene Expression Study:
[0062] To investigate the expression pattern of collagen, real time PCR (RT-PCR) was performed. To this end, the cells were cultured in a similar fashion as described in the sircol collagen assay. After 48 hrs of treatment, the cells were trypsinized and pelleted down prior to RNA isolation. Total mRNA from cells was extracted using a RNA mini kit (Ambion, Life Technologies). Following RNA isolation, hexamer-primed reverse transcription was performed using the High Capacity cDNA Reverse transcription kit (Applied Biosystems). The cDNA was taken forward for RT-PCR against GAPDH and α-SMA (alpha smooth muscle actin), and collagen 1A.
The primer used for α-SMA:

The results of the gene expression study are described below in the following examples.
Example 1: Collagen synthesis assay

[0063] Collagen is an important protein present in the extracellular matrix (ECM) of the skin, produced by dermal fibroblasts, to impart strength and integrity to the skin. As one ages, the synthesis of collagen is reduced and the enzymatic degradation of collagen by matrix metalloproteinase is enhanced, thereby leading to appearance of fine lines and wrinkles which is one of the hallmarks of aging. Enhancing the inherent ability of fibroblast cells to synthesis collagen to retain the tensile strength of skin and consequently delay/reverse the formation of wrinkles, using composition as described herein, is a key objective of the compositions of the present disclosure. To observe the effect of the composition for their ability to enhance the collagen synthesis in skin fibroblast cells, human dermal fibroblasts (HDF’s) were treated with control (water), SAA-HCC 3, SAA-P, and retinoic acid (RA), positive control, in keratinocyte basal medium supplemented with 0.03mM CaCl2 for 48h. SAA-HCC 3 and SAA-P were diluted 1:100 in culture medium for the treatment. Further, the spent medium was collected and the Sircoll assay was performed to detect the collagen secreted by the fibroblast cells. The results of the assay are presented in Figure 1. From the Figure 1 it can be observed that the percentage increase in the amount of collagen synthesized by SAA-P was about 40% higher than the control; and showed an improved tendency in comparison to SAA-HCC 3. The results were found to be almost comparable with the positive control.
[0064] Further, a fold change in collagen gene expression was determined, and the results are presented in Figure 2. For this purpose, HDF were treated with control (water), SAA-HCC 3, SAA-P or retinoic acid (RA) in keratinocyte basal medium supplemented with 0.03mM CaCl2 for 48h and thereafter trypsinized, pelleted down and processed for RT-PCR. From the Figure 2 it can be observed that a 4.5-fold increase in collagen expression was observed with SAA-P, which is much higher than the control, and the test composition, SAA-HCC 3. The results from Figure 1, and Figure 2 substantiate that SAA-P stimulates the synthesis of collagen.
Example 2: α-smooth muscle actin (α-SMA) synthesis assay
[0065] α-SMA regulates the natural lifting properties of the skin by ensuring the
constant equilibrium between skin contraction and relaxation. Goffin et al. (2006)

JCB. 172,2,259-268; and Silver et al. (2003) J Appl Physiol., 95, 2134-2141 suggests that the mechanoreceptors with fibers of α-SMA were under regulated in aged fibroblast, suggesting their weakened response to mechanical stresses, and the subsequent alteration in the regulation of the skins natural lifting properties. Upregulating the expression of α-SMA using composition as described herein, is a key objective of the compositions of the present disclosure. To observe the effect of the composition for their ability to enhance the expression of α-SMA, HDF were treated with control (water), SAA-HCC 3, SAA-P or retinoic acid (RA) in keratinocyte basal medium supplemented with 0.03mM CaCl2 for 48h and thereafter trypsinized, pelleted down and processed for RT-PCR. The results are presented in Figure 3. From the Figure 3 it can be observed that a 3.25-fold increase in α-SMA or stress fibers expression was observed with SAA-P, which is much higher than the test, control, and positive control. The results suggest that SAA-P stimulates the synthesis of α-SMA.
Example 3: Assessment of transparency of the composition of the present
disclosure
[0066] Further, the transparency of the composition over a 48h period was
evaluated with 3 compositions, as provided in Table 3, the results of which are
presented in Figure 4a - 4c.
Table 3

[0067] As can be observed from the Figure 4a - 4c, the composition 3 lost its colourless property after 24 hours, the composition 2 at 120 hours gave a slight yellowish appearance. It can be appreciated that the composition 2 had histidine and the composition 3 had carnosine as part of their composition. It was hypothesized that the african pepper extract may interact with l-histidine, and/or l-carnosine present within the composition, thereby imparting a light yellowish/brown coloration, the color of which intensified with time. The combined observation from composition 2 and 3 led to the composition of the present disclosure (composition 1) which had african pepper extract but devoid of carnosine and histidine. Further, it can be appreciated that the composition 1 remained colourless even after 120 hours (Figure 4c). The enhanced efficacy of the composition of the present disclosure was previously described in Examples 1 and 2.
[0068] From a combined reading of Figure 1, 2 and 3 it can be inferred that the compositions of the present disclosure increase the collagen synthesis and enhances the gene expression of α-SMA in skin fibroblast cells. It can be understood that the composition of the present disclosure can be used in the cosmetic industry to effectively combat skin aging.
Advantages of the present disclosure:
[0069] The present disclosure reveals a transparent skin-beneficial composition comprising: a) aqua (and) glycerine (and) beta-glucan; b) 6-furfurylaminopurine; c) Cyprus rotundus root extract (and) glycerine (and) water; d) Malus domestica fruit

cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water; e) niacinamide; f) sodium hyaluronate; and g) african pepper extract. The composition of the present disclosure can be effectively deployed in formulations catering to the anti-aging market, as it boosts the collagen synthesis - thereby reducing wrinkle formation; and through its enhanced expression of α-smooth muscle actin (SMA) - it can improve skin suppleness. The composition as disclosed in the present disclosure also exhibits transparency and this property can be exploited as a sensorial appeal in arriving at various skin-care formulations comprising the composition of the present disclosure.

I/We Claim:
1. A skin-beneficial composition comprising:
a) aqua (and) glycerine (and) beta-glucan;
b) 6-furfurylaminopurine;
c) Cyprus rotundus root extract (and) glycerine (and) water; and
d) Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water;
e) niacinamide;
f) sodium hyaluronate; and
g) african pepper extract,
wherein the composition is transparent.
2. The skin-beneficial composition as claimed in claim 1, wherein aqua (and) glycerine (and) beta-glucan has a weight percentage in a range of 1-5% with respect to the composition, 6-furfurylaminopurine having a weight percentage in a range of 0.01-0.1% with respect to the composition, Cyprus rotundus root extract (and) glycerine (and) water has a weight percentage in a range of 0.1-2% with respect to the composition, Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water has a weight percentage in a range of 0.05-1% with respect to the composition, niacinamide has a weight percentage in a range of 0.05-5% with respect to the composition, sodium hyaluronate has a weight percentage in a range of 0.01-0.5% with respect to the composition, , african pepper extract has a weight percentage in a range of 0.01-3% with respect to the composition.
3. The skin-beneficial composition as claimed in claim 1, wherein the composition further comprises at least one excipient selected from a group consisting of emollient, humectants, polymers, silicones, preservatives, fragrance, vitamins, chelators, sunscreen agents, moisturization agents, and combinations thereof.
4. A process for preparing the composition as claimed in claim 1, said process comprising:

a) obtaining aqua (and) glycerine (and) beta-glucan;
b) obtaining 6-furfurylaminopurine;
c) obtaining Cyprus rotundus root extract (and) glycerine (and) water; and
d) obtaining Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water;
e) obtaining niacinamide;
f) obtaining sodium hyaluronate;
g) obtaining African pepper extract; and
h) contacting aqua (and) glycerine (and) beta-glucan, 6-
furfurylaminopurine, Cyprus rotundus root extract (and) glycerine (and) water, Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water, niacinamide, sodium hyaluronate, and African pepper extract, to obtain the composition. 5. The process for preparing the composition as claimed in claim 4, said process comprising:
a) obtaining aqua (and) glycerine (and) beta-glucan;
b) obtaining 6-furfurylaminopurine;
c) obtaining Cyprus rotundus root extract (and) glycerine (and) water; and
d) obtaining Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water;
e) obtaining niacinamide;
f) obtaining sodium hyaluronate;
g) obtaining African pepper extract;
h) obtaining at least one excipient; and
i) contacting aqua (and) glycerine (and) beta-glucan, 6-
furfurylaminopurine, Cyprus rotundus root extract (and) glycerine

(and) water, Malus domestica fruit cell culture extract (and) xanthan gum (and) glycerine (and) lecithin (and) phenoxyethanol (and) water, niacinamide, sodium hyaluronate, African pepper extract, and the at least one excipient, to obtain the composition.
6. The composition as claimed in any one of the claims 1-3, wherein the composition increases α-smooth muscle actin (α-SMA) gene expression in a range of 2-5 folds.
7. The composition as claimed in any one of the claims 1-3, wherein the composition increases collagen gene expression in a range of 4-6 folds.
8. The composition as claimed in any one of the claims 1-3, wherein the composition is an anti-ageing composition.