DESC:FORM 2
THE PATENT ACT, 1970
(39 of 1970)
&
The Patent Rules, 2003
COMPLETE SPECIFICATION
(See section 10 and rule 13)

1. TITLE OF THE INVENTION: SPECIALIZED NUTRITION FORMULATION FOR STRESS MANAGEMENT AND SLEEP SUPPORT
2. APPLICANT:
Applicant Name: M/S. AMITOJAS WELLNESS PRIVATE LIMITED
Nationality: INDIAN
Address: A-810, Shweta Aryan, Pipeline road, Jeedimetla, Ranga Reddy Dist, Hyderabad - 500067, Telangana
3. PREAMBLE TO THE DESCRIPTION:
The following specification particularly describes the invention and the manner in which it is to be performed:

4. DESCRIPTION:
Field of the invention:
[0001] The present disclosure generally relates to the technical field of nutritional compositions, and in specific relates a nutritional composition to reduce stress disorders and sleep disorders in individuals suffering from work related stress and insomnia.
Background of the invention:
[0002] Sleep takes up around one-third of a person's life and has an impact on both mental and physical health. It also has an impact on emotion, behaviour, and physiology. Sleep and its regulation are a complicated process that involves a variety of chemicals and brain structures. It is a dynamic process that incorporates both positive and negative signals and involves diverse physiological changes. Due to stress, anxiety, insufficient sleep, and unpredictable sleep patterns linked with a fast-paced and overworked lifestyle, over half of all adults and students suffer from sleep disorders or insomnia. When a regular biological clock, also known as a circadian rhythm or sleep-wake cycle, based on a 24-hour, day/night cycle, cannot be maintained, the above-mentioned problems arise.

[0003] Insomnia is the most frequent and under-reported sleep disorder that is often neglected because of lack of awareness of the impact of such condition in our daily lifestyle. Sleep is one of the essential and basic physiological processes, which is vital for neural development, learning, memory, cardiovascular and metabolic function as well as cellular toxin removal. Insomnia is described as difficulty in initiating or maintaining sleep, or early morning awakening, associated with impaired daytime functioning such as reduced cognitive performance, fatigue or mood disturbances. Insomnia is a widely prevalent condition that can affect anyone, including children, adults and the elderly. Approximately one-third of the adult population occasionally report sleep problems and 6–10% report symptoms that meet the diagnostic criteria for insomnia disorder. Globally, various studies have shown the prevalence of insomnia in 10%–30% of the population, some even as high as 50%–60%.

[0004] Stress isn't a medical diagnosis, so there's no specific treatment for it. However, there are various treatments available for stress management. These treatments include, talking treatments, medication, ecotherapy, complementary and alternative therapies. To access most treatments, the first step is usually to talk with a trained professional. Talking with a trained professional can help an individual to learn to deal with stress and become more aware of their own thoughts and feelings. Common types of talking treatments which can help with stress are Cognitive behavioural therapy and Mindfulness-based stress reduction. The cognitive behavioural therapy (CBT) helps a person to understand their thought patterns, recognise their trigger points and identify positive actions the person can take. The mindfulness-based stress reduction (MBSR), combines mindfulness, meditation and yoga with a particular focus on reducing stress.

[0005] Common approach to the management of insomnia is a combination of cognitive behavioral therapy and pharmacological treatment. Cognitive behavioral therapy (CBT) has a number of advantages. It tends to have a long-acting effect and does not produce the metabolic and adverse-effect burden of medications. On the other hand, the disadvantages of CBT are that it tends to not work quite as quickly as pharmacotherapy. CBT may be the less appropriate approach. In case of pharmacological treatment, most of the hypnotic medications are effective in the management of insomnia. They have a quick onset of action, often producing significant therapeutic benefits upon the very first night of usage. Their main drawbacks include adverse effects such as dependence on the medication, daytime drowsiness, etc., and the tolerance that develops with most agents when used regularly.

[0006] Numerous studies have demonstrated the use of complementary and alternative medicine (CAM) for psychiatric disorders, especially anxiety and sleep disorders. Many studies indicated the importance and efficacy of alternative medicine for treatment of sleep disorders. Meanwhile, nutraceutical therapy is the most commonly used form of complementary and alternative medicine therapies for sleep disorders particularly insomnia. Currently in the market, the products available for sleep support mainly include melatonin supplements which are hormone and hence have additive. Also, long term use of melatonin supplements can cause some side effects including headache, short-term feelings of depression, daytime sleepiness, dizziness, stomach cramps, and irritability.

[0007] Therefore, there is a need for a nutrition composition that aids individuals suffering from stress disorders and insomnia. A nutrition composition is needed that can be added on to food, without altering any taste or smell of the food. There is a need for a nutrition composition that is stable even after adding it to hot foods. There is a need for a nutrition composition that can also be consumed orally or mixed with any other drinks such as milk, fruit juice. There is a need for a nutritional supplement with good efficacy in improving sleep, contains rich nutrition and can meet the nutritional requirement of a human body.
Objectives of the invention:
[0008] The primary objective of the invention is to provide a nutrition composition that aids individuals suffering from stress disorders and insomnia.

[0009] Another objective of the invention is to provide a nutrition composition in the form of a food sprinkle that can be added on to the food, preferably to dinner, and consumed along with the food without altering any taste or smell of the food.

[0010] The other objective of the invention is to provide a process for the preparation of microencapsulated granules of the nutrition composition which are stable even after adding it to hot foods up to a temperature of 80°C.

[0011] Further objective of the invention is to provide food sprinkles of nutritional composition that can also be consumed orally or mixed with any other drinks such as milk, fruit juice.

[0012] Another objective of the invention is to provide a nutritional supplement with good efficacy in improving sleep, contains rich nutrition and can meet the nutritional requirement of a human body.

Summary of the invention:
[0013] The present disclosure proposes a specialized nutrition formulation for stress management and sleep support. The following presents a simplified summary in order to provide a basic understanding of some aspects of the claimed subject matter. This summary is not an extensive overview. It is not intended to identify key/critical elements or to delineate the scope of the claimed subject matter. Its sole purpose is to present some concepts in a simplified form as a prelude to the more detailed description that is presented later.

[0014] In order to overcome the above deficiencies of the prior art, the present disclosure is to solve the technical problem to provide a nutritional composition to reduce stress disorders and sleep disorders in individuals suffering from work related stress and insomnia.

[0015] According to an aspect, the invention provides a nutrition composition for stress management and sleep support. The nutrition composition comprises 4 to 8 weight percentage of hydrolyzed milk proteins, 2 to 6 weight percentage of a blend of amino acids, 0.6 to 1 weight percentage of a neurotransmitter, 0.1 to 0.5 weight percentage of a mixture of neurovitamins, 1 to 5 weight percentage of a mixture of herbal extracts, 1.1 to 3.2 weight percentage of a mineral, 19 to 24 weight percentage of natural bitter masking and stabilizing agents, 0.2 to 0.6 weight percentage of anti-caking agent, and 62 to 66 weight percentage of buffering agent. The proposed composition provides relief from stress disorders and insomnia in individuals.

[0016] In specific, the hydrolyzed milk proteins include Casein Peptides. The blend of amino acids includes a blend of L-Tryptophan, Glycine, and L-Thiamine, thereof. The neurotransmitter includes either Gamma Amino Butyric Acid (GABA) or Di hydroxy Phenyl Alanine (DOPA) thereof. The mixture of neurovitamins includes Vitamin A, Vitamin C, Vitamin E, Vitamin B12, Vitamin B6, Vitamin D3, and Folic acid thereof. In specific, the mixture of herbal extracts includes at least three herbal extracts selected from Ginko Biloba, Chamomile, Ashwagandha, Mucuna Pruriens, Terminalia Arjuna, Bacopa monneri, Lemon Balm, Passion flower, Kava kava, Rhodiola, and Valerian Root thereof. The mineral includes either Magnesium or Calcium thereof. The natural bitter masking and stabilizing agents include at least one natural bitter masking and stabilizing agents such as modified starch, berry extracts, and natural flavours, thereof. The anti-caking agent includes Silicon dioxide, and the buffering agent includes Trisodium Citrate.

[0017] According to another aspect, the invention provides a method for preparation of nutrition composition for stress management and sleep support. First, the hydrolyzed milk proteins, the blend of amino acid, the neurotransmitter, the mixture of neurovitamins, the mixture of herbal extracts, and the mineral are mixed together to obtain a uniform blend. Next, the uniform blend is blended with natural bitter masking and stabilizing agents to obtain a powdered bland tasting mixture. Next, the powdered bland tasting mixture is subjected to microencapsulation process with spray drying under nitrogen atmosphere to obtain spray dried micro granules. Later, the spray dried micro granules are again dried in a vacuum tray drier to obtain granules of nutrition composition with moisture content less than 2% w/w. Finally, the granules of nutrition composition are packed into packets.

[0018] Further, objects and advantages of the present invention will be apparent from a study of the following portion of the specification, the claims, and the attached drawings.
Detailed description of drawings:
[0019] The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate an embodiment of the invention, and, together with the description, explain the principles of the invention.

[0020] FIG. 1 illustrates an exemplary method for preparation of nutrition composition for stress management and sleep support in accordance to an exemplary embodiment of the invention.
Detailed invention disclosure:
[0021] Various embodiments of the present invention will be described in reference to the accompanying drawings. Wherever possible, same or similar reference numerals are used in the drawings and the description to refer to the same or like parts or steps.

[0022] The present disclosure has been made with a view towards solving the problem with the prior art described above, and it is an object of the present invention to provide a nutritional composition to reduce stress disorders and sleep disorders in individuals suffering from work related stress and insomnia.

[0023] According to an exemplary embodiment of the invention, a nutrition composition for stress management and sleep support that provides relief from stress disorders and insomnia in individuals is disclosed. The nutrition composition comprises 4 to 8 weight percentage of hydrolyzed milk proteins, 2 to 6 weight percentage of a blend of amino acids, 0.6 to 1 weight percentage of a neurotransmitter, 0.1 to 0.5 weight percentage of a mixture of neurovitamins, 1 to 5 weight percentage of a mixture of herbal extracts, 1.1 to 3.2 weight percentage of a mineral, 19 to 24 weight percentage of natural bitter masking and stabilizing agents, 0.2 to 0.6 weight percentage of anti-caking agent, 62 to 66 weight percentage of buffering agent, and plurality of excipients.

[0024] In specific, the hydrolyzed milk proteins include Casein Peptides. The blend of amino acids includes a blend of L-Tryptophan, Glycine, and L-Thiamine thereof. The neurotransmitter includes either Gamma Amino Butyric Acid (GABA) or Di hydroxy Phenyl Alanine (DOPA) thereof. The mixture of neurovitamins includes Vitamin A, Vitamin C, Vitamin E, Vitamin B12, Vitamin B6, Vitamin D3, and Folic acid thereof. In specific, the mixture of herbal extracts includes at least three herbal extracts selected from Ginko Biloba, Chamomile, Ashwagandha, Mucuna Pruriens, Terminalia Arjuna, Bacopa monneri, Lemon Balm, Passion flower, Kava kava, Rhodiola, and Valerian Root thereof. The mineral includes either Magnesium or Calcium thereof. The natural bitter masking and stabilizing agents include at least one natural bitter masking and stabilizing agents such as modified starch, berry extracts, and natural flavours thereof. The anti-caking agent includes Silicon dioxide, and the buffering agent includes Trisodium Citrate.

[0025] For instance, table 1 depicts the quantity of each ingredient of the proposed nutrition composition for a serving size of 2 grams.

[0026] Table 1:
Ingredient Quantity for serving (2 gm) Function of the ingredient
Hydrolysed Casein peptides 150 mg Reduces opiod activity in the brain
GABA 20 mg Inhibitory Neurotransmitter
Ginko Biloba 25 mg Brain body coordinator
Vitamin B12 ( cyanocobalamine) 1 mcg Neurovitamin
Vitamin A ( beta carotene) 5 mg Neurovitamin
Vitamin B6 (pyridoxine) 2 mg Neurovitamin
Vitamin D3 ( chole Claciferol) 200 IU Neurovitamin
Mixed Tocopherols 10 mg Neurovitamin
Magnesium 50 mg Reduces stress
Bacopa monneri 25 mg Reduces stress and anxiety
Silicon dioxide 0.01 gm Anti-caking agent
Modified corn starch 0.5 gm Diluent
Valerian Root extract 20 mg Promotes endorphin synthesis
Amino acid Blend ( L- Theanine, Glycine and L-Tryptophan) 100 mg Helps in melatonin and serotonin synthesis
Trisodium Citrate 1.5 gm Buffering agent
Other Excipients q.s Excipients

[0027] According to another exemplary embodiment of the invention, FIG. 1 refers to an exemplary method for preparation of nutrition composition for stress management and sleep support. At step 102, the hydrolyzed milk proteins, the blend of amino acid, the neurotransmitter, the mixture of neurovitamins, the mixture of herbal extracts, and the mineral are mixed together to obtain a uniform blend. At step 104, the uniform blend is blended with natural bitter masking and stabilizing agents to obtain a powdered bland tasting mixture. At step 106, the powdered bland tasting mixture is subjected to microencapsulation process with spray drying under nitrogen atmosphere to obtain spray dried micro granules. At step 108, the spray dried micro granules are again dried in a vacuum tray drier to obtain granules of nutrition composition with moisture content less than 2% w/w. At step 110, the granules of nutrition composition are packed into packets.

[0028] According to another exemplary embodiment of the invention, an interventional, multicenter, prospective, randomized, double-blind, placebo controlled, parallel study is conducted. In the study plan, 70 subjects with insomnia are taken, and duration of study for the study subjects is 30 days. Informed consent is obtained from eligible male and female subjects prior to their enrollment into the study and dispensing of study medication. Eligible subjects are then randomly allocated into two study treatment groups. One group receives the granules of nutrition composition and other group receives a placebo composition. Further, the subjects are asked to return for a planned Visit 2 (14 days) and Visit 3 (30 days) for effectiveness and safety assessments. After the Visit 3 the study is stopped.

[0029] Baseline demographics, medical history, general examination and vital signs of the study subjects are recorded. Efficacy assessment of Insomnia is preformed and the results are recorded. Safety assessments of adverse events and serious adverse events are also recorded. Concomitant illness and concomitant medication if any are also recorded.
A web-based sample size calculation system is utilized to calculate sample size for the study.
[0030] Baseline values of total sleep time in minutes are measured at baseline: Placebo Group (n=214) baseline, total sleep time mean of 315.4, standard deviation (SD) of 5.2. The granules of nutrition composition (n=228) baseline, total sleep time mean of 318.1, SD of 5.0. A mean value of 316.5 minutes and SD of 5.0 minutes are chosen, as these values are towards the central value of the two baseline results.

[0031] An SD of 5.0 with mean of 316.5 implies that the SD is about 0.015 times (base correlation) the size of the mean, which means, the coefficient of variation (CV) is 0. The mean total sleep time in the study is 316.5, and the standard deviation of value 5.0 is assumed. Using the mean total sleep time and standard deviation values as inputs along with 80% power, alpha = 0.05 significance level and assuming the correlation of 0.5, the required total sample size obtained is 60 for evaluation. Allowing 15% drop-out rate, the required sample size for recruitment is total of 70 in 1:1 ratio between Placebo and Active/ granules of nutrition composition (i.e., 35 per treatment group).

[0032] Treatments are prescribed to the subjects by their doctors as part of routine clinical practice. The study treatments are allocated to the subjects randomly through the randomization process. Investigators provide the information regarding the administration of treatment to the subject.

[0033] The study outcomes are taken in two parts as primary efficacy outcome and secondary efficacy outcome. In the primary efficacy outcome, Insomnia Severity Index Score is documented in Case Report Form at baseline, 15th day, and 30th day (comparison of values and changes from baseline within the group and between the group are done).

[0034] In secondary efficacy outcome, first, duration of time taken to fall asleep (in minutes) is documented in the sleep diary at baseline, 15th day, and 30th day. Frequency of awakenings (number of awakenings) is documented in the sleep diary at baseline, 15th day, and 30th day (comparison of values and changes from baseline within the group and between the group are done).

[0035] Duration of awakenings (in minutes) is documented in the sleep diary at baseline, 15th day, and 30th day. Total sleep time (in hours) is documented in the sleep diary at baseline, 15th day, and 30th day. Improvement in quality of sleep (5 point rating) is documented in the sleep diary at baseline, 15th day, and 30th day (comparison of all the documented values and changes from baseline within the group and between the group are done).

[0036] In safety outcome, number of adverse events (AE) or serious adverse events (SAE), either spontaneously reported by the subject, or noticed by the clinician are recorded during the study between the groups. Number of drop-outs between the groups is recorded. Compliance is recorded based on the amount of study medication consumed by the subject since pervious visit between the groups. The safety in terms of vital signs of the subjects is observed between the groups.

[0037] For instance, a tabular representation of the Study Procedures Schedule is presented in table 2.

[0038] Table 2:
Assessment time Visit 1 – Screening and Enrollment Visit Visit 2 – Scheduled Visit Visit 5 – Study Completion
Activity Day 0 + 1 Day 14 ± 2 Day 30 ± 2
Subject information and Informed consent ? - -
Demographics and Medical and medication history ? - -
Vital parameters* ? ? ?
Clinical examination ? ? ?
Screening ? - -
Enrolment ? - -
Inclusion/Exclusion ? - -
Randomization ? - -
Sleep Diary Assessments^ ? ? ?
Questionnaire assessments$ ? ? ?
Adverse Events ? ? ?
Concomitant Illness and Concomitant medication ? ? ?
Study Treatment Dispensing ? ?
Compliance - ? ?
Study Completion/Termination - ? ?
*Vital Signs – Body Temperature, Blood Pressure, Pulse Rate, Respiratory Rate
^Diary Assessments – Sleep Diary
$ Questionnaire assessments – Insomnia Severity Index
Note: Once the subject is enrolled for study participation and if the subject decides to withdraw from the study at any point time before Visit 3 (day 30), all study procedures applicable at Visit 3 will be performed for the subject at the time of study withdrawal.

[0039] Numerous advantages of the present disclosure may be apparent from the discussion above. In accordance with the present disclosure, a nutritional composition to reduce stress disorders and sleep disorders in individuals suffering from work related stress and insomnia is disclosed here.

[0040] The proposed nutrition composition provides relief to individuals suffering from stress disorders and insomnia. The proposed nutrition composition is provided in the form of a food sprinkle that can be added on to the food, which does not alter any taste or smell of the food. The proposed microencapsulated granules of the nutrition composition are stable even after adding it to hot foods up to a temperature of 80°C. The proposed food sprinkles of the nutrition composition can also be consumed orally or mixed with any other drinks such as milk, fruit juice. The proposed nutritional supplement provides good efficacy in improving sleep, contains rich nutrition and can meet the nutritional requirement of a human body.

[0041] It will readily be apparent that numerous modifications and alterations can be made to the processes described in the foregoing examples without departing from the principles underlying the invention, and all such modifications and alterations are intended to be embraced by this application.

5. CLAIMS:
We Claim:
1. A nutrition composition for stress management and sleep support, comprising:
4 to 8 weight percentage of hydrolyzed milk proteins;
2 to 6 weight percentage of a blend of amino acids;
0.6 to 1 weight percentage of a neurotransmitter;
0.1 to 0.5 weight percentage of a mixture of neurovitamins;
1 to 5 weight percentage of a mixture of herbal extracts;
1.1 to 3.2 weight percentage of a mineral;
19 to 24 weight percentage of natural bitter masking and stabilizing agents;
0.2 to 0.6 weight percentage of anti-caking agent; and
62 to 66 weight percentage of buffering agent,
whereby said nutrition composition provides relief from stress disorders and insomnia in individuals.
2. The nutrition composition for stress management and sleep support as claimed in claim 1, wherein said hydrolyzed milk proteins include Casein Peptides.
3. The nutrition composition for stress management and sleep support as claimed in claim 1, wherein said blend of amino acid includes a blend of L-Tryptophan, Glycine, and L-Thiamine, thereof.
4. The nutrition composition for stress management and sleep support as claimed in claim 1, wherein said neurotransmitter includes either Gamma Amino Butyric Acid (GABA) or Di hydroxy Phenyl Alanine (DOPA), thereof.
5. The nutrition composition for stress management and sleep support as claimed in claim 1, wherein said mixture of neurovitamins include Vitamin A, Vitamin C, Vitamin E, Vitamin B12, Vitamin B6, Vitamin D3, and Folic acid, thereof.
6. The nutrition composition for stress management and sleep support as claimed in claim 1, wherein said mixture of herbal extracts includes at least three herbal extracts selected from Ginko Biloba, Chamomile, Ashwagandha, Mucuna Pruriens, Terminalia Arjuna, Bacopa monneri, Lemon Balm, Passion flower, Kava kava, Rhodiola, and Valerian Root, thereof.
7. The nutrition composition for stress management and sleep support as claimed in claim 1, wherein said mineral includes at least one of Magnesium, or Calcium, thereof.
8. The nutrition composition for stress management and sleep support as claimed in claim 1, wherein said natural bitter masking and stabilizing agents include modified starches, berry extracts, and natural flavours, thereof.
9. The nutrition composition for stress management and sleep support as claimed in claim 1, wherein said anti-caking agent includes Silicon dioxide, and said buffering agent includes Trisodium Citrate.
10. A method for preparation of nutrition composition for stress management and sleep support, comprising:
mixing hydrolyzed milk proteins, a blend of amino acid, a neurotransmitter, a mixture of neurovitamins, a mixture of herbal extracts, and a mineral to obtain a uniform blend;
blending said uniform blend with natural bitter masking and stabilizing agents to obtain a powdered bland tasting mixture;
subjecting said powdered bland tasting mixture for microencapsulation process with spray drying under nitrogen atmosphere to obtain spray dried micro granules;
drying said spray dried micro granules in a vacuum tray drier to obtain granules of nutrition composition with moisture content less than 2% w/w, and
packing said granules of nutrition composition into packets.
6. DATE AND SIGNATURE:
Dated this 13th day of January, 2022

7. ABSTRACT:
Title: Specialized Nutrition Formulation for Stress Management and Sleep Support
The present disclosure proposes a specialized nutritional supplement composition for stress management and sleep support for people suffering from stress disorders and insomnia. The proposed nutritional supplement composition has a good efficacy of improving sleep, has rich nutrition and can meet the nutritional requirement of a human body. The proposed food sprinkles of the nutrition composition can also be consumed orally or mixed with any other drinks such as milk, fruit juice. The proposed nutrition composition is provided in the form of a food sprinkle that can be added on to the food, which does not alter any taste or smell of the food.


,CLAIMS:1. A nutrition composition for stress management and sleep support, comprising:
4 to 8 weight percentage of hydrolyzed milk proteins;
2 to 6 weight percentage of a blend of amino acids;
0.6 to 1 weight percentage of a neurotransmitter;
0.1 to 0.5 weight percentage of a mixture of neurovitamins;
1 to 5 weight percentage of a mixture of herbal extracts;
1.1 to 3.2 weight percentage of a mineral;
19 to 24 weight percentage of natural bitter masking and stabilizing agents;
0.2 to 0.6 weight percentage of anti-caking agent; and
62 to 66 weight percentage of buffering agent,
whereby said nutrition composition provides relief from stress disorders and insomnia in individuals.
2. The nutrition composition for stress management and sleep support as claimed in claim 1, wherein said hydrolyzed milk proteins include Casein Peptides.
3. The nutrition composition for stress management and sleep support as claimed in claim 1, wherein said blend of amino acid includes a blend of L-Tryptophan, Glycine, and L-Thiamine, thereof.
4. The nutrition composition for stress management and sleep support as claimed in claim 1, wherein said neurotransmitter includes either Gamma Amino Butyric Acid (GABA) or Di hydroxy Phenyl Alanine (DOPA), thereof.
5. The nutrition composition for stress management and sleep support as claimed in claim 1, wherein said mixture of neurovitamins include Vitamin A, Vitamin C, Vitamin E, Vitamin B12, Vitamin B6, Vitamin D3, and Folic acid, thereof.
6. The nutrition composition for stress management and sleep support as claimed in claim 1, wherein said mixture of herbal extracts includes at least three herbal extracts selected from Ginko Biloba, Chamomile, Ashwagandha, Mucuna Pruriens, Terminalia Arjuna, Bacopa monneri, Lemon Balm, Passion flower, Kava kava, Rhodiola, and Valerian Root, thereof.
7. The nutrition composition for stress management and sleep support as claimed in claim 1, wherein said mineral includes at least one of Magnesium, or Calcium, thereof.
8. The nutrition composition for stress management and sleep support as claimed in claim 1, wherein said natural bitter masking and stabilizing agents include modified starches, berry extracts, and natural flavours, thereof.
9. The nutrition composition for stress management and sleep support as claimed in claim 1, wherein said anti-caking agent includes Silicon dioxide, and said buffering agent includes Trisodium Citrate.
10. A method for preparation of nutrition composition for stress management and sleep support, comprising:
mixing hydrolyzed milk proteins, a blend of amino acid, a neurotransmitter, a mixture of neurovitamins, a mixture of herbal extracts, and a mineral to obtain a uniform blend;
blending said uniform blend with natural bitter masking and stabilizing agents to obtain a powdered bland tasting mixture;
subjecting said powdered bland tasting mixture for microencapsulation process with spray drying under nitrogen atmosphere to obtain spray dried micro granules;
drying said spray dried micro granules in a vacuum tray drier to obtain granules of nutrition composition with moisture content less than 2% w/w, and
packing said granules of nutrition composition into packets.