The present invention is used for inspection of the presence or absence of diseased to stomach cancer in a subject, specific miRNA and specific detection kit or device of gastric cancer, including possible binding nucleic acids, and the using the nucleic acid measuring the expression level of the miRNA method for detecting gastric cancer, including.

Background technique

[0002]

　Stomach is a bag-shaped digestive organs leading to the esophagus, once they pooled the food that came from the esophagus, responsible for the first stage of gastric secretion to digestion play a role. Stomach, near the inlet is the cardia lead to esophageal, pyloric portion near the outlet leading to the duodenum, the other portion are divided into the gastric body (Non-Patent Document 1). According to the site-specific cancer morbidity and mortality statistics in this country by the National Research and Development Institute of the National Cancer Research Center for Cancer Control and Information Center is to disclose, sufferers of stomach cancer in 2010, male 86,728 people, women 39 It is estimated that a total of 125,730 people of 002 people. Also number of deaths in 2012 due to stomach cancer in men 32,206 people, is the 16,923 people of a total of 49,129 women, has become the second leading cause of death by the Japanese of cancer. In addition, in the United States, affected with 22,220 people gastric cancer in 2014, it is estimated that 10,990 people will die by gastric cancer (Non-Patent Document 1).

[0003]

　The degree of progress of the gastric cancer is defined in the Non-Patent Document 2, tumor size, invasive, lymph node metastasis, stage 0, IA, such as by distant metastasis, IB, IIA, IIB, IIIA, IIIB, IIIC, in IV being classified. The 5-year relative survival rate for gastric cancer is highly dependent on the degree of progress of the cancer, the stage I 57 ~ 71%, in stage II 33 ~ 46%, in stage III 9 ~ 20%, is reported as 4% in stage IV and have (non-Patent Document 1). Therefore, since finding the stomach cancer at an early stage leads to improved survival rate, providing a means for allowing early detection has been desired strongly.

[0004]

　Surgical therapy for the treatment of gastric cancer, drug therapy or radiation therapy are used in combination. In particular, in the there is no doubt a very early stage of lymph node metastasis of gastric cancer, endoscopic mucosal resection (Endoscopic Mucosal Resection, EMR) and endoscopic submucosal dissection (Endoscopic Submucosal Dissection, ESD) case is adaptable There are many, it is possible to treat without placing a burden on the patient.

[0005]

　With the aim of early detection of gastric cancer, in men and women over 40 years old in Japan, gastric cancer screening once a year is recommended. The effectiveness of "Stomach X-ray inspection" as the method for gastric cancer screening is shown, the result of the X-ray inspection, when the workup is required, gastroscopy is performed. In addition, due to the discovery of stomach cancer, CT, PET, have also been utilized diagnostic imaging such as MRI (non-patent document 1).

[0006]

　On the other hand, blood markers for gastric cancer screening has not been established. The protein tumor markers, such as CEA and CA19-9 in the serum has been suggested to be associated with gastric cancer (Non-patent document 3), there is no sufficient evidence to recommend the use as a screening applications. On the other hand, albeit at the research stage, as shown in Patent Documents 1-3, the expression level of a micro RNA in a biological sample, including blood (miRNA), or expression of the expression level and other protein markers of miRNA by combining the amount, it is reported that detects a stomach cancer.

[0007]

　Patent Document 1 has been shown a method of detecting cancer comprising a gastric cancer using hsa-miR-125a-3p in the blood.

[0008]

　hsa-miR-23a-3p of Patent Document 2 blood and tissue, miR-92-1, miR-92-2 (miR-92a-1-3p, miR-92a-2-3p), as well as, miR- 128b (miR-128-2-3p), miR-30c (miR-30c-5p), miR-135-1, miR-135-2 (miR-135a-5p) and miR-149 (miR-149-5p ) using other miRNAs, a method for detecting the gastric cancer is shown.

[0009]

　Patent Document 3 has been shown a method of detecting a gastric cancer using hsa-miR-451 and 468 in the blood (hsa-miR-468-5p).

CITATION

Patent literature

[0010]

Patent Document 1: International Publication No. 2010/062706
Patent Document 2: Laid-open Publication No. 2014-060993
Patent Document 3: Unexamined Publication No. 2013-085542

Non-patent literature

[0011]

非特許文献1 : Ａｍｅｒｉｃａｎ Ｃａｎｃｅｒ Ｓｏｃｉｅｔｙ 「Ｓｔｏｍａｃｈ Ｃａｎｃｅｒ」、２０１３年監修、ｐ．３、６、１８～２０ｈｔｔｐ：／／ｗｗｗ．ｃａｎｃｅｒ．ｏｒｇ／ａｃｓ／ｇｒｏｕｐｓ／ｃｉｄ／ｄｏｃｕｍｅｎｔｓ／ｗｅｂｃｏｎｔｅｎｔ／００３１４１－ｐｄｆ．ｐｄｆ
非特許文献2 : Ｓｏｂｉｎ，　Ｌ．ら、「ＴＮＭ Ｃｌａｓｓｉｆｉｃａｔｉｏｎ ｏｆ ＭａｌｉｇｎａｎｔＴｕｍｏｕｒｓ　第7版　日本語版」２０１０年、ｐ．６９～７３
非特許文献3 : Ｋｉｍ，Ｈ．Ｊ．ら、Ａｃｔａ　Ｏｎｃｏｌｏｇｉｃａ、２００９年、　４８巻、ｐ．３８５～３９０

Summary of the invention

Problems that the Invention is to Solve

[0012]

　An object of the present invention is to find a novel gastric cancer tumor marker is to provide a method capable of effectively detecting the gastric cancer using specific bindable nucleic acid the marker. The primary inspection of stomach cancer, including the stomach X-ray inspection, which is commonly used in Japan, some CT, PET, the image tests, such as MRI (Non-Patent Document 1). However, in Japan, gastric cancer still accounts for 2 cause of death of cancer, not necessarily the image inspection is possible to suppress the gastric cancer death.

[0013]

　The tumor marker for gastric cancer detection, for example, CEA, are known, such as CA19-9, in general, as shown in Non-Patent Document 3, 5 ng / mL for CEA, for CA19-9 37U / mL is used as a reference value. However, these tumor markers, recurrence or of gastric cancer, but it may help to confirm the therapeutic effect, because it is very little that the expression in the early stage of gastric cancer is increased, is useful for the purposes of as gastric cancer screening It is that there is no. Furthermore, CEA, tumor markers, such as CA19-9, because it can be increased even for reasons other than gastric cancer, only these markers are said to can not be determined whether the gastric cancer. In addition, if resulting in a diagnosis of gastric cancer by mistake the other cancers, or missed the opportunity of appropriate treatment, wrong unnecessary economic to the patient in applying the medical, it will be forced to physically burden.

[0014]

　Further, albeit at the research stage, but reported that determines stomach cancer using the expression level of the micro-RNA in a biological sample, including blood (miRNAs) are in the following, none of put to practical use.

[0015]

　Patent Document 1 has been shown a method of detecting cancer comprising a gastric cancer using hsa-miR-125a-3p and other miRNA in the blood. However, this detection method, specific accuracy to determine gastric cancer, sensitivity, there is no description about the detection performance such as specificity, poor industrial practicality.

[0016]

　hsa-miR-23a-3p of Patent Document 2, blood and tissue, miR-92-1, miR-92-2 (miR-92a-1-3p, miR-92a-2-3p) using, also Furthermore miR-128 (miR-128-2-5p), miR-30c (miR-30c-5p), miR-135-1, miR-135-2 (miR-135a-5p), miR-149 (miR- 149-5p) and using other miRNAs, a method for detecting the gastric cancer is described.

[0017]

　Among this, hsa-miR-23a-3p, miR-92-1, miR-92-2 (miR-92a-1-3p, miR-92a-2-3p) For, miRNA, especially for detection of gastric cancer It has been described as. However, there is no description that was carried out verification of the markers in the blood, specific detection examples are intended for miRNA in the organization, because this is not an easy screening test, poor industrial practicality.

[0018]

　Thus, in the detection of gastric cancer, either because an existing tumor markers its performance is low, and the detection methods and performance for markers of research stage are not specifically shown, in the case of using them, healthy body implementation and wasteful additional testing due to the erroneous detection and gastric cancer patients, there is a possibility that the loss occurs in the therapeutic opportunity by overlooking the gastric cancer patients. Further, since the measuring miRNA consisting of several tens to several hundreds to increase inspection costs, difficult to use for large scale screening in health examination or the like. Further, since high undesirable invasive to the patient is taking a gastric tissue to measure tumor markers, can be detected from the blood can be collected in a minimally invasive, it can be correctly judged gastric cancer patients and healthy body, high gastric cancer marker precision is required. In particular, because of the current challenges, such as radiation exposure and high costs for screening according to an image being inspected such as the stomach X-ray examination for early detection of gastric cancer, to provide a more convenient gastric cancer primary screening test It is believed to lead to the interests of the subjects and medical administration.

Means for Solving the Problems

[0019]

　The present inventors have made intensive studies to solve the above problems, found several genes that are available from blood can be collected in less invasive to the detection marker of gastric cancer, used capable of specifically binding a nucleic acid thereto by, it found that stomach cancer can be significantly detected, thereby completing the present invention.

[0020]

　In other words, the present invention has the following features.
(1) is a gastric cancer marker, miR-4257, miR-6726-5p , miR-1343-3p, miR-1247-3p, miR-6787-5p, miR-6875-5p, miR-1225-3p, miR- 8063, miR-6781-5p, miR- 4746-3p, miR-1908-5p, miR-6756-5p, miR-204-3p, miR-4651, miR-6757-5p, miR-6825-5p, miR- 7108-5p, miR-4792, miR- 7641, miR-3188, miR-3131, miR-6780b-5p, miR-8069, miR-6840-3p, miR-8072, miR-1233-5p, miR-6887- 5p, miR-1231, miR- 5572, miR-6738-5p, miR-6784-5p, miR-6791-5p, miR-6749-5p, miR-6741-5p, miR-128-1-5p, miR- 4419b, miR-6746-5p, miR- 3184-5p, miR-3679-5p, miR-7110-5p, miR-4516, miR-6717-5p, miR-6826-5p, miR-4433b-3p, miR- 3679-3p, miR-3135b, miR- 3622a-5p, miR-711, miR-4467, miR-6857-5p, miR-6515-3p, miR-1225-5p, miR-187-5p, miR-3185, miR-642b-3p, miR- 1249, miR-744-5p, miR-4442, miR-1228-3p, miR-939-5p, miR-6845-5p, miR-887-3p, miR-7845-5p, miR-6729-5p, miR-4632-5p, miR-615-5p, miR-6724-5p, miR-4728-5p, miR-6732-5p, miR-6816-5p, miR-4695-5p, miR- 6088, miR-7975, miR- 3197, miR-6125, miR-4433-3p, miR-6727-5p, miR-4706, miR-7847-3p, miR-6805-3p, miR-6766-3p, miR- 1913, miR-4649-5p, miR- 602, miR-3663-3p, miR-6893-5p, miR-6861-5p, miR-4449, miR-6842-5p, miR-4454, miR-5195-3p, miR-663b, miR-6765-5p, miR-4513, miR-614, miR-6785-5p, miR-6777-5p, miR-940, miR-4741, miR-6870-5p, miR-6131, miR- 150-3p, miR-4707-5p, miR- 1915-3p, miR-3937, miR-937-5p, miR-4443, miR-1914-3p, miR-3620-5p, miR-1268b, miR-1227- 5p, miR-6880-5p, miR- 4417, miR-6802-5p, miR-6769a-5p, miR-663a, miR-6721-5p, miR-4532, miR-7977, miR-92b-5p, miR- 371a-5p, miR-6126, miR-4734, miR-4665-3p, miR-423-5p, miR-1469, miR-4675, miR-1915-5p, miR-6716-5p, miR-718, miR- 4281, miR-6820-5p, miR- 6795-5p, miR-6779-5p, miR-7109-5p, miR-6798-5p, miR-4648, miR-8059, miR-6765-3p, miR-6132, miR-4492, miR-7107-5p, miR-3195, miR-3180, miR-296-3p, miR-564, miR-1268a, miR-6848-5p, miR-762, miR-2861, miR-1203, miR-1260b, miR-4476, miR-6885-5p, miR-6769b-5p, miR-23b-3p, miR-1343-5p, miR-3621, miR-4688, miR-4286, miR-4640-5p, miR-4739, miR-1260a, miR-4276, miR-7106-5p, miR-6794-5p, miR-6774-5p, miR-4707-3p, miR-4534, miR-4294, miR-6850-5p, miR-6089 and at least one or more polynucleotides that specifically comprising bondable nucleic acid detection kit for gastric cancer selected from the group consisting of miR-671-5p.

[0021]

(2) miR-4257 is hsa-miR-4257, miR-6726-5p is hsa-miR-6726-5p, miR-1343-3p is hsa-miR-1343-3p, miR-1247 -3p is hsa-miR-1247-3p, miR-6787-5p is hsa-miR-6787-5p, miR-6875-5p is hsa-miR-6875-5p, miR-1225-3p there is a hsa-miR-1225-3p, miR-8063 is hsa-miR-8063, miR-6781-5p is hsa-miR-6781-5p, miR-4746-3p is hsa-miR-4746 a -3p, miR-1908-5p is hsa-miR-1908-5p, miR-6756-5p is hsa-miR-6756-5p, miR-204-3p is hsa-miR-204-3p at is, miR-4651 is hsa-miR-4651, miR-6757-5p is hsa-miR-6757-5p, miR-6825-5p is hsa-miR-6825-5p, miR-7108 -5p is hsa-miR-7108-5p, miR-4792 is hsa-miR-4792, miR-7641 is hsa-miR-7641, miR-3188 is hsa-miR-3188, miR -3131 is hsa-miR-3131, miR-6780b-5p is hsa-miR-6780b-5p, miR-8069 is hsa-miR-8069, miR-6840-3p is hsa-miR-6840 a -3p, miR-8072 is hsa-miR-8072, miR-1233-5p is hsa-miR-1233-5p, miR-6887-5p is hsa-miR-6887-5p, miR -1231 is hsa-miR-1231, miR-5572 is hsa-miR-5572, miR-6738-5p is hsa-miR-6738-5p, miR-6784-5p is hsa-miR-6784 a -5p, miR-6791-5p is hsa-miR-6791-5p, miR-6749-5p is hsa-miR-6749-5p, miR-6741-5p is hsa-miR-6741-5p at is, miR-128-1-5p is hsa-miR-128-1-5p, miR-4419b is hsa-miR-4419b, miR-6746-5p is located in the hsa-miR-6746-5p , miR-3184-5p is hsa-miR-3184-5p, miR-3679-5p is hsa-miR-3679-5p, miR-7110-5p is hsa-miR-7110-5p, miR -4516 is hsa-miR-4516, miR-6717-5p is hsa-miR-6717-5p, miR-6826-5p is hsa-miR-6826-5p, the miR-4433b-3p hsa a -miR-4433b-3p, miR-3679-3p is hsa-miR-3679-3p, miR-3135b is hsa-miR-3135b, miR-3622a-5p is hsa-miR-3622a-5p at is, miR-711 is hsa-miR-711, miR-4467 is hsa-miR-4467, miR-6857-5p is hsa-miR-6857-5p, miR-6515-3p is hsa -miR-6515-3p is, miR-1225-5p is hsa-miR-1225-5p, miR-187-5p is hsa-miR-187-5p, miR-3185 is hsa-miR-3185 at is, miR-642b-3p is hsa-miR-642b-3p, miR-1249 is hsa-miR-1249, miR-744-5p is hsa-miR-744-5p, miR-4442 there is a hsa-miR-4442, miR-1228-3p is hsa-miR-1228-3p, miR-939-5p is hsa-miR-939-5p, miR-6845-5p is hsa-miR a -6845-5p, miR-887-3p is hsa-miR-887-3p, miR-7845-5p is hsa-miR-7845-5p, miR-6729-5p is hsa-miR-6729 a -5p, miR-4632-5p is hsa-miR-4632-5p, miR-615-5p is hsa-miR-615-5p, miR-6724-5p is hsa-miR-6724-5p at is, miR-4728-5p is hsa-miR-4728-5p, miR-6732-5p is hsa-miR-6732-5p, miR-6816-5p is located in the hsa-miR-6816-5p , miR-4695-5p is hsa-miR-4695-5p, miR-6088 is hsa-miR-6088, miR-7975 is hsa-miR-7975, miR-3197 is hsa-miR-3197 at is, miR-6125 is hsa-miR-6125, miR-4433-3p is hsa-miR-4433-3p, miR-6727-5p is hsa-miR-6727-5p, miR-4706 there is a hsa-miR-4706, miR-7847-3p is hsa-miR-7847-3p, miR-6805-3p is hsa-miR-6805-3p, miR-6766-3p is hsa-miR a -6766-3p, miR-1913 is hsa-miR-1913, miR-4649-5p is hsa-miR-4649-5p, miR-602 is hsa-miR-602, miR-3663 -3p is hsa-miR-3663-3p, miR-6893-5p is hsa-miR-6893-5p, miR-6861-5p is hsa-miR-6861-5p, the miR-4449 hsa a -miR-4449, miR-6842-5p is hsa-miR-6842-5p, miR-4454 is hsa-miR-4454, miR-5195-3p is located in the hsa-miR-5195-3p , miR-663b is hsa-miR-663b, miR-6765-5p is hsa-miR-6765-5p, miR-4513 is hsa-miR-4513, miR-614 is hsa-miR-614 at is, miR-6785-5p is hsa-miR-6785-5p, miR-6777-5p is hsa-miR-6777-5p, miR-940 is hsa-miR-940, miR-4741 there is a hsa-miR-4741, miR-6870-5p is hsa-miR-6870-5p, miR-6131 is hsa-miR-6131, miR-150-3p is hsa-miR-150-3p at is, miR-4707-5p is hsa-miR-4707-5p, miR-1915-3p is hsa-miR-1915-3p, miR-3937 is hsa-miR-3937, miR-937 -5p is hsa-miR-937-5p, miR-4443 is hsa-miR-4443, miR-1914-3p is hsa-miR-1914-3p, miR-3620-5p is hsa-miR a -3620-5p, miR-1268b is hsa-miR-1268b, miR-1227-5p is hsa-miR-1227-5p, miR-6880-5p is located in the hsa-miR-6880-5p , miR-4417 is hsa-miR-4417, miR-6802-5p is hsa-miR-6802-5p, miR-6769a-5p is hsa-miR-6769a-5p, the miR-663a hsa a -miR-663a, miR-6721-5p is hsa-miR-6721-5p, miR-4532 is hsa-miR-4532, miR-7977 is hsa-miR-7977, miR-92b -5p is hsa-miR-92b-5p, miR-371a-5p is hsa-miR-371a-5p, miR-6126 is hsa-miR-6126, miR-4734 is hsa-miR-4734 at is, miR-4665-3p is hsa-miR-4665-3p, miR-423-5p is hsa-miR-423-5p, miR-1469 is hsa-miR-1469, miR-4675 there is a hsa-miR-4675, miR-1915-5p is hsa-miR-1915-5p, miR-6716-5p is hsa-miR-6716-5p, miR-718 is hsa-miR-718 at is, miR-4281 is hsa-miR-4281, miR-6820-5p is hsa-miR-6820-5p, miR-6795-5p is hsa-miR-6795-5p, miR-6779 -5p is hsa-miR-6779-5p, miR-7109-5p is hsa-miR-7109-5p, miR-6798-5p is hsa-miR-6798-5p, the miR-4648 hsa a -miR-4648, miR-8059 is hsa-miR-8059, miR-6765-3p is hsa-miR-6765-3p, miR-6132 is hsa-miR-6132, miR-4492 there is a hsa-miR-4492, miR-7107-5p is hsa-miR-7107-5p, miR-3195 is hsa-miR-3195, miR-3180 is hsa-miR-3180, miR -296-3p is hsa-miR-296-3p, miR-564 is hsa-miR-564, miR-1268a is hsa-miR-1268a, miR-6848-5p is hsa-miR-6848 a -5p, miR-762 is hsa-miR-762, miR-2861 is hsa-miR-2861, miR-1203 is hsa-miR-1203, miR-1260b is hsa-miR-1260b at is, miR-4476 is hsa-miR-4476, miR-6885-5p is hsa-miR-6885-5p, miR-6769b-5p is hsa-miR-6769b-5p, miR-23b -3p is hsa-miR-23b-3p, miR-1343-5p is hsa-miR-1343-5p, miR-3621 is hsa-miR-3621, miR-4688 is hsa-miR-4688 at is, miR-4286 is hsa-miR-4286, miR-4640-5p is hsa-miR-4640-5p, miR-4739 is hsa-miR-4739, miR-1260a is hsa-miR a -1260a, miR-4276 is hsa-miR-4276, miR-7106-5p is hsa-miR-7106-5p, miR-6794-5p is hsa-miR-6794-5p, miR -6774-5p is hsa-miR-6774-5p, miR-4707-3p is hsa-miR-4707-3p, miR-4534 is hsa-miR-4534, miR-4294 is hsa-miR is -4294, miR-6850-5p is hsa-miR-6850-5p, miR-6089 is hsa-miR-6089, and, miR-671-5p is a hsa-miR-671-5p the kit according to (1).

[0022]

(3) wherein the nucleic acid is poly shown in the following (a) ~ (e)
nucleotides: (a) u In the nucleotide sequence or the nucleotide sequence shown in any of SEQ ID NOs 165 and 635-642 are t a polynucleotide consisting of the nucleotide sequence is, the variant, derivative, or fragment thereof comprising 15 or more continuous nucleotides,
a nucleotide sequence shown in any one of (b) SEQ ID NOs 165 and 635-642 polynucleotides, comprising
(c) SEQ ID NOs 165 and 635 u in the nucleotide sequence or the nucleotide sequence represented by any one of 642 consisting of a nucleotide sequence complementary to the nucleotide sequence is t polynucleotide variants body, derivatives thereof, or a fragment comprising 15 or more continuous nucleotides,
nucleotide sequence u is t in the nucleotide sequence or the nucleotide sequence represented by any one of (d) SEQ ID NOs 165 and 635-642 polynucleotide, and a nucleotide sequence complementary to
(e) above (a) ~ (d) any of the polynucleotides under stringent conditions with a polynucleotide hybridizing,
polynucleotide selected from the group consisting of in a kit according to (1) or (2).

[0023]

(4) The kit is another of gastric cancer marker, miR-128-2-5p, miR-125a-3p, at least 1, which is selected from the group consisting of miR-92a-2-5p and miR-486-3p one or more further comprises a polynucleotide specifically bindable nucleic acid kit according to any one of (1) to (3).

[0024]

(5) miR-128-2-5p is hsa-miR-128-2-5p, miR-125a-3p is hsa-miR-125a-3p, miR-92a-2-5p is hsa-miR a -92a-2-5p, and, miR-486-3p is hsa-miR-486-3p, kit according to (4).

[0025]

(6) said nucleic acid, the following (f) ~ shown in (j) a
polynucleotide: (f) the nucleotide sequence u is t in the nucleotide sequence or the nucleotide sequence represented by any of SEQ ID NO: 166-169 polynucleotide consisting, variants, derivatives thereof, or a fragment comprising 15 or more continuous nucleotides,
polynucleotides comprising a nucleotide sequence represented by any one of (g) SEQ ID NO: 166 ~ 169,
(h) sequences No. 166 polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence u is t in the nucleotide sequence or the nucleotide sequence represented by any one of 169, a variant, derivative, or 15 or more contiguous bases a fragment thereof comprising a,
(i) SEQ ID NO: 166 polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence u is t in the nucleotide sequence or the nucleotide sequence represented by any one of 169, and
(j) the (f) any of the polynucleotides under stringent conditions with a polynucleotide hybridizing of ~ (i),
a polynucleotide selected from the group consisting of a kit according to (4) or (5).

[0026]

(7) The kit is another of gastric cancer marker, miR-3196, miR-211-3p, miR-4271, miR-6851-5p, miR-149-3p, miR-4667-5p, miR-135a- 3p, miR-4486, miR-4697-5p, miR-4725-3p, miR-6510-5p, miR-5001-5p, miR-4673, miR-4466, miR-23a-3p, miR-3656, miR- 6782-5p, miR-4689, miR-451a, miR-4446-3p, miR-3180-3p, miR-642a-3p, miR-6889-5p, miR-3178, miR-4665-5p, miR-6722- 3p, further comprising miR-30c-1-3p, the miR-4507, miR-3141 and at least one polynucleotide capable of specifically binding nucleic acid is selected from the group consisting of miR-1199-5p, ( the kit according to any one of 1) to (6).

[0027]

(8) miR-3196 is hsa-miR-3196, miR-211-3p is hsa-miR-211-3p, miR-4271 is hsa-miR-4271, miR-6851-5p is hsa a -miR-6851-5p, miR-149-3p is hsa-miR-149-3p, miR-4667-5p is hsa-miR-4667-5p, miR-135a-3p is hsa-miR a -135a-3p, miR-4486 is hsa-miR-4486, miR-4697-5p is hsa-miR-4697-5p, miR-4725-3p is located in the hsa-miR-4725-3p , miR-6510-5p is hsa-miR-6510-5p, miR-5001-5p is hsa-miR-5001-5p, miR-4673 is hsa-miR-4673, is miR-4466 hsa a -miR-4466, miR-23a-3p is hsa-miR-23a-3p, miR-3656 is hsa-miR-3656, miR-6782-5p is located in the hsa-miR-6782-5p , miR-4689 is hsa-miR-4689, miR-451a is hsa-miR-451a, miR-4446-3p is hsa-miR-4446-3p, miR-3180-3p is hsa-miR a -3180-3p, miR-642a-3p is hsa-miR-642a-3p, miR-6889-5p is hsa-miR-6889-5p, miR-3178 is located in the hsa-miR-3178 , miR-4665-5p is hsa-miR-4665-5p, miR-6722-3p is hsa-miR-6722-3p, miR-30c-1-3p is hsa-miR-30c-1-3p at is, miR-4507 is hsa-miR-4507, miR-3141 is hsa-miR-3141, and, miR-1199-5p is hsa-miR-1199-5p, according to (7) kit.

[0028]

(9) said nucleic acid, the following (k) ~ (o) are shown
polynucleotide: (k) the nucleotide sequence u is t in the nucleotide sequence or the nucleotide sequence represented by any of SEQ ID NO: 170-199 polynucleotide consisting, variants, derivatives thereof, or a fragment comprising 15 or more continuous nucleotides,
polynucleotides comprising a nucleotide sequence represented by any of (l) SEQ ID NO: 170. ~ 199,
(yd) sequence No. 170 polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence u is t in the nucleotide sequence or the nucleotide sequence represented by any one of 199, a variant, derivative, or 15 or more contiguous bases a fragment containing the
polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence that u is t in the nucleotide sequence or the nucleotide sequence represented by any one of (n) SEQ ID NO: 170-199, and
(o) the (k) any of the polynucleotides under stringent conditions with a polynucleotide hybridizing of ~ (n),
is a polynucleotide selected from the group consisting of a kit according to (7) or (8).

[0029]

(10) The kit comprises at least two or more nucleic acids respectively capable of specifically binding at least two or more polynucleotides selected from all the gastric cancer marker listed in (1) or (2), (1) the kit according to any one of (9).

[0030]

(11) 胃 が ん マ ー カ ー で あ る, miR-4257, miR-6726-5p, miR-1343-3p, miR-1247-3p, miR-6787-5p, miR-6875-5p, miR-1225-3p, miR 8063, miR-6781-5p, miR-4746-3p, miR-1908-5p, miR-6756-5p, miR-204-3p, miR-4651, miR-6757-5p, miR-6825-5p, miR 7108-5p, miR-4792, miR-7641, miR-3188, miR-3131, miR-6780b-5p, miR-8069, miR-6840-3p, miR-8072, miR-1233-5p, miR-6887- 5p, miR-1231, miR-5572, miR-6738-5p, miR-6784-5p, miR-6791-5p, miR-6749-5p, miR-6741-5p, miR-128-1-5p, miR 4419b, miR-6746-5p, miR-3184-5p, miR-3679-5p, miR-7110-5p, miR-4516, miR-6717-5p, miR-6826-5p, miR-4433b-3p, miR 3679-3p, miR-3135b, miR-3622a-5p, miR-711, miR-4467, miR-6857-5p, miR-6515-3p, miR-1225-5p, miR-187-5p, miR-3185, miR-642b-3p, miR-1249, miR-744-5p, miR-4442, miR-1228-3p, miR-939-5p, miR-6845-5p, miR-887-3p, miR-7845-5p, miR-6729-5p, miR-4632-5p, miR-615-5p, miR-6724-5p, miR-4728-5p, miR-6732-5p, miR-6816-5p, miR-4695-5p, miR 6088, miR-7975, miR-3197, miR-6125, miR-4433-3p, miR-6727-5p, miR-4706, miR-7847-3p, miR-6805-3p, miR-6766-3p, miR 1913 miR-4649-5p, miR-602, miR-3663-3p, miR-6893-5p, miR-6861-5p, miR-4449, miR-6842-5p, miR-4454, miR-5195-3p, miR-663b, miR-6765-5p, miR-4513, miR-614, miR-6785-5p, miR-6777-5p, miR-940, miR-4741, miR-6870-5p, miR-6131, miR 150-3p, miR-4707-5p, miR-1915-3p, miR-3937, miR-937-5p, miR-4443, miR-1914-3p, miR-3620-5p, miR-1268b, miR-1227- 5p, miR-6880-5p, miR-4417, miR-6802-5p, miR-6769a-5p, miR-663a, miR-6721-5p, miR-4532, miR-7977, miR-92b-5p, miR 371a-5p, miR-6126, miR-4734, miR-4665-3p, miR-423-5p, miR-1469, miR-4675, miR-1915-5p, miR-6716-5p, miR-718, miR 4281, miR-6820-5p, miR-6795-5p, miR-6779-5p, miR-7109-5p, miR-6798-5p, miR-4648, miR-8059, miR-6765-3p, miR-6132, miR-4492, miR-7107-5p, miR-3195, miR-3180, miR-296-3p, miR-564, miR-1268a, miR-6848-5p, miR-762, miR-2861, miR-1203, miR-1260b, miR-4476, miR-6885-5p, miR-6769b-5p, miR-23b-3p, miR-1343-5p, miR-3621, miR-4688, miR-4286, miR-4640-5p, miR-4739, miR-1260a, miR-4276, miR-7106-5p, miR-6794-5p, miR-6774-5p, miR-4707-3p, miR-4534, miR-4294, miR-6850-5p, miR-6089 及 び miR-671-5p か ら な る 群 か ら 選 択 さ れ る 少 な く と も 1 つ 以上 の ポ リ ヌ ク レ オ チ ド と 特異 的 に 結合 可能 な 核酸 を 含 む, 胃 が ん の 検 出 用 デ バ イ ス.

[0031]

(12) miR-4257 is hsa-miR-4257, miR-6726-5p is hsa-miR-6726-5p, miR-1343-3p is hsa-miR-1343-3p, miR-1247 -3p is hsa-miR-1247-3p, miR-6787-5p is hsa-miR-6787-5p, miR-6875-5p is hsa-miR-6875-5p, miR-1225-3p there is a hsa-miR-1225-3p, miR-8063 is hsa-miR-8063, miR-6781-5p is hsa-miR-6781-5p, miR-4746-3p is hsa-miR-4746 a -3p, miR-1908-5p is hsa-miR-1908-5p, miR-6756-5p is hsa-miR-6756-5p, miR-204-3p is hsa-miR-204-3p at is, miR-4651 is hsa-miR-4651, miR-6757-5p is hsa-miR-6757-5p, miR-6825-5p is hsa-miR-6825-5p, miR-7108 -5p is hsa-miR-7108-5p, miR-4792 is hsa-miR-4792, miR-7641 is hsa-miR-7641, miR-3188 is hsa-miR-3188, miR -3131 is hsa-miR-3131, miR-6780b-5p is hsa-miR-6780b-5p, miR-8069 is hsa-miR-8069, miR-6840-3p is hsa-miR-6840 a -3p, miR-8072 is hsa-miR-8072, miR-1233-5p is hsa-miR-1233-5p, miR-6887-5p is hsa-miR-6887-5p, miR -1231 is hsa-miR-1231, miR-5572 is hsa-miR-5572, miR-6738-5p is hsa-miR-6738-5p, miR-6784-5p is hsa-miR-6784 a -5p, miR-6791-5p is hsa-miR-6791-5p, miR-6749-5p is hsa-miR-6749-5p, miR-6741-5p is hsa-miR-6741-5p at is, miR-128-1-5p is hsa-miR-128-1-5p, miR-4419b is hsa-miR-4419b, miR-6746-5p is located in the hsa-miR-6746-5p , miR-3184-5p is hsa-miR-3184-5p, miR-3679-5p is hsa-miR-3679-5p, miR-7110-5p is hsa-miR-7110-5p, miR -4516 is hsa-miR-4516, miR-6717-5p is hsa-miR-6717-5p, miR-6826-5p is hsa-miR-6826-5p, the miR-4433b-3p hsa a -miR-4433b-3p, miR-3679-3p is hsa-miR-3679-3p, miR-3135b is hsa-miR-3135b, miR-3622a-5p is hsa-miR-3622a-5p at is, miR-711 is hsa-miR-711, miR-4467 is hsa-miR-4467, miR-6857-5p is hsa-miR-6857-5p, miR-6515-3p is hsa -miR-6515-3p is, miR-1225-5p is hsa-miR-1225-5p, miR-187-5p is hsa-miR-187-5p, miR-3185 is hsa-miR-3185 at is, miR-642b-3p is hsa-miR-642b-3p, miR-1249 is hsa-miR-1249, miR-744-5p is hsa-miR-744-5p, miR-4442 there is a hsa-miR-4442, miR-1228-3p is hsa-miR-1228-3p, miR-939-5p is hsa-miR-939-5p, miR-6845-5p is hsa-miR a -6845-5p, miR-887-3p is hsa-miR-887-3p, miR-7845-5p is hsa-miR-7845-5p, miR-6729-5p is hsa-miR-6729 a -5p, miR-4632-5p is hsa-miR-4632-5p, miR-615-5p is hsa-miR-615-5p, miR-6724-5p is hsa-miR-6724-5p at is, miR-4728-5p is hsa-miR-4728-5p, miR-6732-5p is hsa-miR-6732-5p, miR-6816-5p is located in the hsa-miR-6816-5p , miR-4695-5p is hsa-miR-4695-5p, miR-6088 is hsa-miR-6088, miR-7975 is hsa-miR-7975, miR-3197 is hsa-miR-3197 at is, miR-6125 is hsa-miR-6125, miR-4433-3p is hsa-miR-4433-3p, miR-6727-5p is hsa-miR-6727-5p, miR-4706 there is a hsa-miR-4706, miR-7847-3p is hsa-miR-7847-3p, miR-6805-3p is hsa-miR-6805-3p, miR-6766-3p is hsa-miR a -6766-3p, miR-1913 is hsa-miR-1913, miR-4649-5p is hsa-miR-4649-5p, miR-602 is hsa-miR-602, miR-3663 -3p is hsa-miR-3663-3p, miR-6893-5p is hsa-miR-6893-5p, miR-6861-5p is hsa-miR-6861-5p, the miR-4449 hsa a -miR-4449, miR-6842-5p is hsa-miR-6842-5p, miR-4454 is hsa-miR-4454, miR-5195-3p is located in the hsa-miR-5195-3p , miR-663b is hsa-miR-663b, miR-6765-5p is hsa-miR-6765-5p, miR-4513 is hsa-miR-4513, miR-614 is hsa-miR-614 at is, miR-6785-5p is hsa-miR-6785-5p, miR-6777-5p is hsa-miR-6777-5p, miR-940 is hsa-miR-940, miR-4741 there is a hsa-miR-4741, miR-6870-5p is hsa-miR-6870-5p, miR-6131 is hsa-miR-6131, miR-150-3p is hsa-miR-150-3p at is, miR-4707-5p is hsa-miR-4707-5p, miR-1915-3p is hsa-miR-1915-3p, miR-3937 is hsa-miR-3937, miR-937 -5p is hsa-miR-937-5p, miR-4443 is hsa-miR-4443, miR-1914-3p is hsa-miR-1914-3p, miR-3620-5p is hsa-miR a -3620-5p, miR-1268b is hsa-miR-1268b, miR-1227-5p is hsa-miR-1227-5p, miR-6880-5p is located in the hsa-miR-6880-5p , miR-4417 is hsa-miR-4417, miR-6802-5p is hsa-miR-6802-5p, miR-6769a-5p is hsa-miR-6769a-5p, the miR-663a hsa a -miR-663a, miR-6721-5p is hsa-miR-6721-5p, miR-4532 is hsa-miR-4532, miR-7977 is hsa-miR-7977, miR-92b -5p is hsa-miR-92b-5p, miR-371a-5p is hsa-miR-371a-5p, miR-6126 is hsa-miR-6126, miR-4734 is hsa-miR-4734 at is, miR-4665-3p is hsa-miR-4665-3p, miR-423-5p is hsa-miR-423-5p, miR-1469 is hsa-miR-1469, miR-4675 there is a hsa-miR-4675, miR-1915-5p is hsa-miR-1915-5p, miR-6716-5p is hsa-miR-6716-5p, miR-718 is hsa-miR-718 at is, miR-4281 is hsa-miR-4281, miR-6820-5p is hsa-miR-6820-5p, miR-6795-5p is hsa-miR-6795-5p, miR-6779 -5p is hsa-miR-6779-5p, miR-7109-5p is hsa-miR-7109-5p, miR-6798-5p is hsa-miR-6798-5p, the miR-4648 hsa a -miR-4648, miR-8059 is hsa-miR-8059, miR-6765-3p is hsa-miR-6765-3p, miR-6132 is hsa-miR-6132, miR-4492 there is a hsa-miR-4492, miR-7107-5p is hsa-miR-7107-5p, miR-3195 is hsa-miR-3195, miR-3180 is hsa-miR-3180, miR -296-3p is hsa-miR-296-3p, miR-564 is hsa-miR-564, miR-1268a is hsa-miR-1268a, miR-6848-5p is hsa-miR-6848 a -5p, miR-762 is hsa-miR-762, miR-2861 is hsa-miR-2861, miR-1203 is hsa-miR-1203, miR-1260b is hsa-miR-1260b at is, miR-4476 is hsa-miR-4476, miR-6885-5p is hsa-miR-6885-5p, miR-6769b-5p is hsa-miR-6769b-5p, miR-23b -3p is hsa-miR-23b-3p, miR-1343-5p is hsa-miR-1343-5p, miR-3621 is hsa-miR-3621, miR-4688 is hsa-miR-4688 at is, miR-4286 is hsa-miR-4286, miR-4640-5p is hsa-miR-4640-5p, miR-4739 is hsa-miR-4739, miR-1260a is hsa-miR a -1260a, miR-4276 is hsa-miR-4276, miR-7106-5p is hsa-miR-7106-5p, miR-6794-5p is hsa-miR-6794-5p, miR -6774-5p is hsa-miR-6774-5p, miR-4707-3p is hsa-miR-4707-3p, miR-4534 is hsa-miR-4534, miR-4294 is hsa-miR is -4294, miR-6850-5p is hsa-miR-6850-5p, miR-6089 is hsa-miR-6089, and, miR-671-5p is a hsa-miR-671-5p a device according to (11).

[0032]

(13) wherein the nucleic acid is poly shown in the following (a) ~ (e)
nucleotides: (a) u In the nucleotide sequence or the nucleotide sequence shown in any of SEQ ID NOs 165 and 635-642 are t a polynucleotide consisting of the nucleotide sequence is, the variant, derivative, or fragment thereof comprising 15 or more continuous nucleotides,
a nucleotide sequence shown in any one of (b) SEQ ID NOs 165 and 635-642 polynucleotides, comprising
(c) SEQ ID NOs 165 and 635 u in the nucleotide sequence or the nucleotide sequence represented by any one of 642 consisting of a nucleotide sequence complementary to the nucleotide sequence is t polynucleotide variants body, derivatives thereof, or a fragment comprising 15 or more continuous nucleotides,
nucleotide sequence u is t in the nucleotide sequence or the nucleotide sequence represented by any one of (d) SEQ ID NOs 165 and 635-642 polynucleotide, and a nucleotide sequence complementary to
(e) above (a) ~ (d) any of the polynucleotides under stringent conditions with a polynucleotide hybridizing,
polynucleotide selected from the group consisting of at is, the device described in (11) or (12).

[0033]

(14) The device is another of gastric cancer marker, miR-128-2-5p, miR-125a-3p, at least 1, which is selected from the group consisting of miR-92a-2-5p and miR-486-3p one or more poly further comprising a nucleotide binding specifically to nucleic acid, according to any one of (11) to (13) device.

[0034]

(15) miR-128-2-5p is hsa-miR-128-2-5p, miR-125a-3p is hsa-miR-125a-3p, miR-92a-2-5p is hsa-miR a -92a-2-5p, and, miR-486-3p is hsa-miR-486-3p, as described in (14) devices.

[0035]

(16) said nucleic acid, the following (f) ~ shown in (j) a
polynucleotide: (f) the nucleotide sequence u is t in the nucleotide sequence or the nucleotide sequence represented by any of SEQ ID NO: 166-169 polynucleotide consisting, variants, derivatives thereof, or a fragment comprising 15 or more continuous nucleotides,
polynucleotides comprising a nucleotide sequence represented by any one of (g) SEQ ID NO: 166 ~ 169,
(h) sequences No. 166 polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence u is t in the nucleotide sequence or the nucleotide sequence represented by any one of 169, a variant, derivative, or 15 or more contiguous bases a fragment thereof comprising a,
(i) SEQ ID NO: 166 polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence u is t in the nucleotide sequence or the nucleotide sequence represented by any one of 169, and
(j) the (f) ~ any polynucleotide and a polynucleotide hybridizing under stringent conditions (i),
a polynucleotide selected from the group consisting of, according to (14) or (15) device.

[0036]

(17) The device is another of gastric cancer marker, miR-3196, miR-211-3p, miR-4271, miR-6851-5p, miR-149-3p, miR-4667-5p, miR-135a- 3p, miR-4486, miR-4697-5p, miR-4725-3p, miR-6510-5p, miR-5001-5p, miR-4673, miR-4466, miR-23a-3p, miR-3656, miR- 6782-5p, miR-4689, miR-451a, miR-4446-3p, miR-3180-3p, miR-642a-3p, miR-6889-5p, miR-3178, miR-4665-5p, miR-6722- 3p, further comprising miR-30c-1-3p, the miR-4507, miR-3141 and at least one polynucleotide capable of specifically binding nucleic acid is selected from the group consisting of miR-1199-5p, ( device according to any one of 11) - (16).

[0037]

(18) miR-3196 is hsa-miR-3196, miR-211-3p is hsa-miR-211-3p, miR-4271 is hsa-miR-4271, miR-6851-5p is hsa a -miR-6851-5p, miR-149-3p is hsa-miR-149-3p, miR-4667-5p is hsa-miR-4667-5p, miR-135a-3p is hsa-miR a -135a-3p, miR-4486 is hsa-miR-4486, miR-4697-5p is hsa-miR-4697-5p, miR-4725-3p is located in the hsa-miR-4725-3p , miR-6510-5p is hsa-miR-6510-5p, miR-5001-5p is hsa-miR-5001-5p, miR-4673 is hsa-miR-4673, is miR-4466 hsa a -miR-4466, miR-23a-3p is hsa-miR-23a-3p, miR-3656 is hsa-miR-3656, miR-6782-5p is located in the hsa-miR-6782-5p , miR-4689 is hsa-miR-4689, miR-451a is hsa-miR-451a, miR-4446-3p is hsa-miR-4446-3p, miR-3180-3p is hsa-miR a -3180-3p, miR-642a-3p is hsa-miR-642a-3p, miR-6889-5p is hsa-miR-6889-5p, miR-3178 is located in the hsa-miR-3178 , miR-4665-5p is hsa-miR-4665-5p, miR-6722-3p is hsa-miR-6722-3p, miR-30c-1-3p is hsa-miR-30c-1-3p at is, miR-4507 is hsa-miR-4507, miR-3141 is hsa-miR-3141, and, miR-1199-5p is hsa-miR-1199-5p, according to (17) of the device.

[0038]

(19) said nucleic acid, the following (k) ~ (o) are shown
polynucleotide: (k) the nucleotide sequence u is t in the nucleotide sequence or the nucleotide sequence represented by any of SEQ ID NO: 170-199 polynucleotide consisting, variants, derivatives thereof, or a fragment comprising 15 or more continuous nucleotides,
polynucleotides comprising a nucleotide sequence represented by any of (l) SEQ ID NO: 170. ~ 199,
(yd) sequence No. 170 polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence u is t in the nucleotide sequence or the nucleotide sequence represented by any one of 199, a variant, derivative, or 15 or more contiguous bases a fragment containing the
polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence that u is t in the nucleotide sequence or the nucleotide sequence represented by any one of (n) SEQ ID NO: 177-199, and
(o) the (k) any of the polynucleotides under stringent hybridizing polynucleotides under conditions of ~ (n),
is a polynucleotide selected from the group consisting of, according to (17) or (18) device.

[0039]

(20) wherein the device is a device for measuring by hybridization techniques, according to any one of (11) to (19) device.

[0040]

(21) The hybridization techniques, a nucleic acid array technology, as described in (20) device.

[0041]

(22) wherein the device comprises at least two or more nucleic acids respectively capable of specifically binding at least two or more polynucleotides selected from all the gastric cancer markers described (11) or (12), device according to any one of (11) to (21).

[0042]

(23) (1) Kit or (11) according to any one of (10) using a device according to any of (22), measuring the expression level of a target nucleic acid in the specimen of a subject, using the expression level that is the measurement, and a control expression level of the healthy body, measured similarly, that the subject is suffering from gastric cancer, or be assessed in vitro a not to be suffering from gastric cancer including a method for detecting gastric cancer.

[0043]

(24) wherein the subject is a human, the method described in (23).

[0044]

(25) wherein the analyte is blood, serum or plasma, the method described in (23) or (24).

[0045]

　Terms used herein have the following definitions.

[0046]

　Nucleotide, polynucleotide, DNA, by abbreviations such as RNA shall be in accordance with customary in the "Guidelines for the creation of specification and the like, including the nucleotide sequence or amino acid sequence" (Japan Patent Office ed.) And the art .

[0047]

　A "polynucleotide" as used herein, RNA, DNA, and none of the RNA / DNA (chimeric) used for encompasses nucleic acids. Incidentally, the above-mentioned DNA, include cDNA, genomic DNA, and even synthetic DNA is. Also included in the above RNA, total RNA, include mRNA, rRNA, miRNA, siRNA, snoRNA, snRNA, none of the non-coding (non-coding) RNA and synthetic RNA are. "Synthetic DNA" and "synthetic RNA" as used herein based on the predetermined nucleotide sequence (either the native sequence or non-naturally occurring sequence good.), For example using an automated nucleic acid synthesizer, artificially It refers fabricated DNA and RNA. "Non-naturally occurring sequence" as used herein, are intended to be used in the broad sense, differs from the native sequence, for example, substitution of one or more nucleotides, deletion, insertion and / or sequence comprising additional ( that includes mutated sequence), the sequence comprising one or more modified nucleotides (i.e., a modified sequence), and the like. In this description, a polynucleotide is used interchangeably with nucleic acid.

[0048]

　The term "fragment" as used herein, is a polynucleotide having a nucleotide sequence of contiguous portion of a polynucleotide, 15 nucleotides or more, it is desirable that preferably 17 bases or more, more preferably having 19 or more bases in length .

[0049]

　"Gene" as used herein, RNA, and not double-stranded DNA only, include the single-stranded DNA, such as a plus strand constituting it (or sense strand) or complementary strand (or antisense strand) used is intended to. Also not to be particularly limited by its length.

[0050]

　Thus, a "gene", as used herein, unless otherwise stated, a double-stranded DNA, one strand having a single-stranded DNA (plus strand) complementary to sequence the positive strand containing a cDNA containing the human genomic DNA DNA (complementary strand), micro-RNA (miRNA), and their fragments, and none of these transcripts contains. Also the "gene" is not only a "gene" represented by a specific nucleotide sequence (or a SEQ ID NO), RNA RNA and biological functions encoded by these are equivalent, for example, homologs (i.e., homologs or orthologs), encompasses genetic variants such as polymorphisms, and "nucleic acid" encoding derivatives. Such homologues, the "nucleic acid" which encodes the variant or derivative, in particular, under stringent conditions as described later, a nucleotide sequence, or the base represented by any of SEQ ID NOs 657 u has a nucleotide sequence, the complementary sequence that hybridizes to the nucleotide sequence is t in the sequence can be cited "nucleic acid". The "gene" does not ask another functional area, may include for example an expression control region, coding region, and exon or intron. Also, "gene" may be included in the cell, are released to the outside of cells may be present alone, it may be in a state of being encapsulated in the vesicles, called exosomes.

[0051]

　The "exosomes" in the present specification (also known as "exosomes"), is a vesicle that was wrapped in a lipid bilayer membrane, which is secreted from the cell. Exosomes derived from multivesicular endosomes, may be when they are released into the extracellular environment, including RNA, biological materials, such as "gene" and protein such as DNA therein. Exosomes are known blood, serum, plasma, may be included in a body fluid such as lymph.

[0052]

　The term "transcription product" as used herein, refers to a DNA sequence of a gene of RNA synthesized as a template. Binds to a site where RNA polymerase is referred to as the promoter upstream of the gene, RNA is synthesized in a manner going as 3 'end is bound ribonucleotides be complementary to the base sequence of DNA. This RNA not only the gene itself, the expression control region, the coding region, contains the entire sequence from the transcription start site, including exons or introns up to the end of the poly (A) sequence.

[0053]

　Further, "micro RNA (miRNAs)", as used herein, unless otherwise noted, are transcribed as an RNA precursor of the hairpin-like structure, is cleaved by the dsRNA cleavage enzyme having RNase III cleavage activity, a protein complex termed RISC incorporated into and used with the intention of non-coding RNA between 15 and 25 bases involved in mRNA translation inhibition. Also "miRNA" as used herein not only "miRNA" represented by a specific nucleotide sequence (or a SEQ ID NO), the precursor of the "miRNA" (pre-miRNA, pri-miRNA), and these miRNA biological functions are equivalent, for example, homologs (i.e., homologs or orthologs), variants such as polymorphisms, and also encompasses derivatives. Such precursors, congeners, the variant or derivative, in particular, can be identified by miRBase release 20 (http://www.mirbase.org/), under stringent conditions as described later , can be cited "miRNA" having any complementary sequences which hybridize to the nucleotide sequence of the specific nucleotide sequence shown in any of SEQ ID NOs 657. Furthermore, "miRNA" as used herein may be a gene product of miR gene, such gene products are mature miRNA (e.g., involved in translational repression of the mRNA as described above 15 ~ 25 include a base, or 19 to 25 bases, non-coding RNA) or miRNA precursor (for example, the pre-miRNA or pri-miRNA, such as a).

[0054]

　The term "probe" as used herein encompasses polynucleotides and / or complementary to the polynucleotide used for specifically detecting the resulting RNA or polynucleotides derived therefrom by expression of a gene.

[0055]

　The term "primer" as used herein encompasses polynucleotides and / or complementary to the polynucleotide specifically recognizes the resulting RNA or polynucleotides derived therefrom by expression of the gene is amplified.

[0056]

　Wherein complementary polynucleotide (complementary strand, reverse strand) to the base sequence u is t in the nucleotide sequence or the nucleotide sequence as defined by any of SEQ ID NOS: 1 to 657 from the consisting of polynucleotides full-length sequence, or a partial sequence, (for convenience here, this is referred to as positive strand) the a for: T (U), G: based on base pairing relationships such C, in a base complementary relationship It refers to a polynucleotide. However, such a complementary strand is not limited to the case of forming the base sequence and completely complementary sequence of the plus strand of interest, as it has a complementary relationship to the extent that can hybridize with the positive strand and the stringent conditions of interest there may be.

[0057]

　The "stringent conditions" as used herein, to a greater extent than the nucleic acid probe to other sequences (e.g. background standard error × 2 or more measurements of the average + background measurement value of the measurement value), the It refers to hybridizing conditions to the target sequence. Stringent conditions are sequence-dependent and will be different depending on the environment the hybridization is performed. By controlling the stringency of the hybridization and / or washing conditions, target sequences can be identified is 100% complementary to the nucleic acid probe. Specific examples of the "stringent conditions" will be described later.

[0058]

　The "Tm value" as used herein, the double-stranded portion of the polynucleotide is denatured into single-stranded, double-stranded and single-stranded 1: means the temperature which is present in a ratio.

[0059]

　A "variant" as used herein, the case of nucleic acid, polymorphism variants of native caused such mutation, or any one of the nucleotide sequences of SEQ ID NOs 657 or the u in the nucleotide sequence nucleotide sequence which is t, or one or more base deletion in its partial sequence, substitution, addition or variant comprising an insertion, or the respective nucleotide sequence or a partial sequence of about 90%, about 95% or more, about 97%, about 98% or more, variant represents about 99% of the percent identity or hybridize under stringent conditions of the nucleotide sequence or a polynucleotide or oligonucleotide as previously defined comprising the partial sequence It means soybean nucleic acid.

[0060]

　The term "several" as used herein, means about 10,9,8,7,6,5,4,3 or two integers.

[0061]

　As used herein, mutants, site-directed mutagenesis, can be fabricated using well known techniques such as mutagenesis method using PCR method.

[0062]

　"% Identity" herein using the search system of the protein or gene according to the above BLAST or FASTA, by introducing gaps, or not introducing gaps, can be determined (Zheng Zhang et al., ... 2000, J Comput Biol, 7 Volume, p203-214;. Altschul, S.F et al., 1990, Journal of Molecular Biology, 215 volume, p403-410;. Pearson, W.R et al., 1988 year, Proc. Natl. Acad. Sci. U.S.A., 85th Volume, p2444-2448).

[0063]

　The term "derivative" as used herein, modified nucleic acids include, but are not limited to for example, labeled derivatives due fluorophore, modified nucleotides (e.g. halogen, alkyl such as methyl, alkoxy such as methoxy, thio, groups such as carboxymethyl reconstruction of nucleotides and bases including double bond saturation, deamination, derivatives including nucleotides, etc.) that has received and substitution of sulfur molecules of oxygen molecules, PNA (peptide nucleic acid; Nielsen, P.E. et al., 1991, Science, 254 vol, p1497-500), LNA (locked nucleic acid;. Obika, S et al., refers to 1998, Tetrahedron Lett, 39 vol, p5401-5404), and the like..

[0064]

　"Nucleic acid" capable of binding to a polynucleotide specifically selected from miRNA is a gastric cancer marker described hereinabove is a nucleic acid that is synthesized or prepared, in particular, "nucleic acid probe" or "primer" hints, to detect the presence or absence of gastric cancer in a subject, or the presence or absence of onset of gastric cancer, the extent of the affected, the degree of presence and improved improvement of gastric cancer, for diagnosing a susceptibility to treatment of gastric cancer, or prevention of gastric cancer, to screen for useful candidates for amelioration or treatment is directly or indirectly utilized. In vivo in relation to these morbidity of stomach cancer, particularly blood, specifically recognize and bind the transcription product or cDNA thereof synthetic nucleic acid represented by any one of the sequences in the sample Nos. 1 to 657 body fluids such as urine nucleotides capable of, including oligonucleotides and polynucleotides. These nucleotides, oligonucleotides and polynucleotides, in a living body based on the nature, as a probe for detecting the gene expressed in such tissues or the cells and also for amplifying the gene expressed in vivo it can be effectively used as a primer.

[0065]

　The term "detection" as used herein, testing, measurement, detection, or, may be replaced by the term decision support. Further, the term "evaluation" herein is used in its sense including at supporting diagnosis or evaluation based on the inspection results or measurement results.

[0066]

　"Subject" as used herein includes humans, primates, including chimpanzees, dogs, pet animals such as cats, cows, horses, sheep, domestic animals such as goats, mice, rodents such as rats It means of a mammal. Further, "normal form" is also a such mammalian, means an animal not suffering from cancer to be detected.

[0067]

　Probability is used "P" or "P-value" in the statistical test, the extreme statistic is observed than statistics calculated from actual data under the null hypothesis herein It is shown. Thus as the "P" or "P-value" is small, it considered that there are significant differences between the comparison.

[0068]

　As used herein, "sensitivity" means the value of (number of true positives) / (Number of Number + false negative true positives). Sensitivity becomes possible to find the stomach cancer at an early stage is higher, leading to a decrease in full cancers of resection and recurrence rate.

[0069]

　As used herein, "specificity" refers to the (number of true negatives) / (Number of Number + false positive true negative). If the higher specificity of a healthy body to prevent the implementation of the useless additional testing due to misjudged and gastric cancer patients, leading to a reduction of relief and medical expenses of the patient's burden.

[0070]

　As used herein, "accuracy" means the value of (the number of number + True negatives of true positives) / (total number of cases). Accuracy shows the percentage determination result is correct with respect to all the samples, the first index to evaluate the detection performance.

[0071]

　Judgment in the present specification, and becomes a "specimen" is the subject of detection or diagnosis, refers to the occurrence of gastric cancer, the progression of gastric cancer, and the organization and the biological material gene of the present invention is to expression changes with the exertion of therapeutic effect on gastric cancer . Vascular gastric tissue and around the in particular, organ lymph nodes and organs, also metastasis is suspected, skin, and blood, urine, saliva, sweat, body fluids such as tissue exudate, sera prepared from blood, plasma refers to other, feces, hair and the like. Furthermore the biological sample, specifically extracted from these refers to a gene, such as RNA or miRNA.

[0072]

　The term as used herein, "hsa-miR-4257 gene" or "hsa-miR-4257" is, hsa-miR-4257 gene described in SEQ ID NO: 1 (miRBase Accession No.MIMAT0016878) and other species It encompasses such homologs or orthologs. hsa-miR-4257 gene can be obtained Goff LA et al., 2009, PLoS One, 4 vol., by the method described in e7192. Further, "hsa-miR-4257" takes a hairpin-like structure "hsa-mir-4257" (miRBase Accession No.MI0015856, SEQ ID NO: 200) is known as its precursor.

[0073]

　The term as used herein, "hsa-miR-6726-5p gene" or "hsa-miR-6726-5p" is, hsa-miR-6726-5p gene (miRBase Accession described in SEQ ID NO: 2 No. MIMAT0027353) and other, including such species homologs or orthologs. hsa-miR-6726-5p gene can be obtained Ladewig E et al., 2012, Genome Res, 22 vol., by the method described in p1634-1645. Further, "hsa-miR-6726-5p" takes a hairpin-like structure "hsa-mir-6726" (miRBase Accession No.MI0022571, SEQ ID NO: 201) is known as its precursor.

[0074]

　The term as used herein, "hsa-miR-1343-3p gene" or "hsa-miR-1343-3p" is, hsa-miR-1343-3p gene (miRBase Accession described in SEQ ID NO: 3 No. MIMAT0019776) and other, including such species homologs or orthologs. hsa-miR-1343-3p gene can be obtained Persson H et al., 2011,. Cancer Res, 71 vol., by the method described in p78-86. Further, "hsa-miR-1343-3p" takes a hairpin-like structure "hsa-mir-1343" (miRBase Accession No.MI0017320, SEQ ID NO: 202) is known as its precursor.

[0075]

　The term as used herein, "hsa-miR-1247-3p gene" or "hsa-miR-1247-3p" is, hsa-miR-1247-3p gene (miRBase Accession described in SEQ ID NO: 4 No. MIMAT0022721) and other, including such species homologs or orthologs. hsa-miR-1247-3p gene, Morin RD et al., 2008, Genome Res, 18 vol., can be obtained by the method described in p610-621. Further, "hsa-miR-1247-3p" takes a hairpin-like structure "hsa-mir-1247" (miRBase Accession No.MI0006382, SEQ ID NO: 203) is known as its precursor.

[0076]

　The term as used herein, "hsa-miR-6787-5p gene" or "hsa-miR-6787-5p" is, hsa-miR-6787-5p gene (miRBase Accession described in SEQ ID NO: 5 of 5 No. MIMAT0027474) and other, including such species homologs or orthologs. hsa-miR-6787-5p gene can be obtained Ladewig E et al., 2012, Genome Res, 22 vol., by the method described in p1634-1645. Further, "hsa-miR-6787-5p" takes a hairpin-like structure "hsa-mir-6787" (miRBase Accession No.MI0022632, SEQ ID NO: 204) is known as its precursor.

[0077]

　The term as used herein, "hsa-miR-6875-5p gene" or "hsa-miR-6875-5p" is, hsa-miR-6875-5p gene (miRBase Accession described in SEQ ID NO. 6 No. MIMAT0027650) and other, including such species homologs or orthologs. hsa-miR-6875-5p gene can be obtained Ladewig E et al., 2012, Genome Res, 22 vol., by the method described in p1634-1645. Further, "hsa-miR-6875-5p" takes a hairpin-like structure "hsa-mir-6875" (miRBase Accession No.MI0022722, SEQ ID NO: 205) is known as its precursor.

[0078]

　The term as used herein, "hsa-miR-1225-3p gene" or "hsa-miR-1225-3p" is, hsa-miR-1225-3p gene (miRBase Accession described in SEQ ID NO. 7 No. MIMAT0005573) and other, including such species homologs or orthologs. hsa-miR-1225-3p gene, Berezikov E et al., 2007, MoI the Cell, 28 vol, may be obtained by the method described in p328-336. Further, "hsa-miR-1225-3p" takes a hairpin-like structure "hsa-mir-1225" (miRBase Accession No.MI0006311, SEQ ID NO: 206) is known as its precursor.

[0079]

　The term as used herein, "hsa-miR-8063 gene" or "hsa-miR-8063" is, hsa-miR-8063 gene described in SEQ ID NO: 8 (miRBase Accession No.MIMAT0030990) and other species It encompasses such homologs or orthologs. hsa-miR-8063 gene, Wang HJ, et al., 2013, Shock, 39 vol, may be obtained by the method described in p480-487. Further, "hsa-miR-8063" takes a hairpin-like structure "hsa-mir-8063" (miRBase Accession No.MI0025899, SEQ ID NO: 207) is known as its precursor.

[0080]

　The term as used herein, "hsa-miR-6781-5p gene" or "hsa-miR-6781-5p" is, hsa-miR-6781-5p gene (miRBase Accession according to SEQ ID NO. 9 No. MIMAT0027462) and other, including such species homologs or orthologs. hsa-miR-6781-5p gene can be obtained Ladewig E et al., 2012, Genome Res, 22 vol., by the method described in p1634-1645. Further, "hsa-miR-6781-5p" takes a hairpin-like structure "hsa-mir-6781" (miRBase Accession No.MI0022626, SEQ ID NO: 208) is known as its precursor.

[0081]

　The term as used herein, "hsa-miR-4746-3p gene" or "hsa-miR-4746-3p" is, hsa-miR-4746-3p gene (miRBase Accession described in SEQ ID NO: 10 No. MIMAT0019881) and other, including such species homologs or orthologs. hsa-miR-4746-3p gene can be obtained Persson H et al., 2011,. Cancer Res, 71 vol., by the method described in p78-86. Further, "hsa-miR-4746-3p" takes a hairpin-like structure "hsa-mir-4746" (miRBase Accession No.MI0017385, SEQ ID NO: 209) is known as its precursor.

[0082]

　The term as used herein, "hsa-miR-1908-5p gene" or "hsa-miR-1908-5p" is, hsa-miR-1908-5p gene (miRBase Accession described in SEQ ID NO. 11 No. MIMAT0007881) and other, including such species homologs or orthologs. hsa-miR-1908-5p gene, Bar M et al., 2008, Stem The Cells, 26 vol, may be obtained by the method described in p2496-2505. Further, "hsa-miR-1908-5p" takes a hairpin-like structure "hsa-mir-1908" (miRBase Accession No.MI0008329, SEQ ID NO: 210) is known as its precursor.

[0083]

　The term as used herein, "hsa-miR-6756-5p gene" or "hsa-miR-6756-5p" is, hsa-miR-6756-5p gene (miRBase Accession described in SEQ ID NO. 12 No. MIMAT0027412) and other, including such species homologs or orthologs. hsa-miR-6756-5p gene can be obtained Ladewig E et al., 2012, Genome Res, 22 vol., by the method described in p1634-1645. Further, "hsa-miR-6756-5p" takes a hairpin-like structure "hsa-mir-6756" (miRBase Accession No.MI0022601, SEQ ID NO: 211) is known as its precursor.

[0084]

　The term as used herein, "hsa-miR-204-3p gene" or "hsa-miR-204-3p" is, hsa-miR-204-3p gene (miRBase Accession described in SEQ ID NO. 13 No. MIMAT0022693) and other, including such species homologs or orthologs. hsa-miR-204-3p gene, Lim LP, et al., 2003, Science, 299 vol, may be obtained by the method described in p1540. Further, "hsa-miR-204-3p" takes a hairpin-like structure "hsa-mir-204" (miRBase Accession No.MI0000284, SEQ ID NO: 212) is known as its precursor.

[0085]

　The term as used herein, "hsa-miR-4651 gene" or "hsa-miR-4651" is, hsa-miR-4651 gene described in SEQ ID NO: 14 (miRBase Accession No.MIMAT0019715) and other species It encompasses such homologs or orthologs. hsa-miR-4651 gene can be obtained Persson H et al., 2011,. Cancer Res, 71 vol., by the method described in p78-86. Further, "hsa-miR-4651" takes a hairpin-like structure "hsa-mir-4651" (miRBase Accession No.MI0017279, SEQ ID NO: 213) is known as its precursor.

[0086]

　The term as used herein, "hsa-miR-6757-5p gene" or "hsa-miR-6757-5p" is, hsa-miR-6757-5p gene (miRBase Accession described in SEQ ID NO: 15 No. MIMAT0027414) and other, including such species homologs or orthologs. hsa-miR-6757-5p gene can be obtained Ladewig E et al., 2012, Genome Res, 22 vol., by the method described in p1634-1645. Further, "hsa-miR-6757-5p" takes a hairpin-like structure "hsa-mir-6757" (miRBase Accession No.MI0022602, SEQ ID NO: 214) is known as its precursor.

[0087]

　The term as used herein, "hsa-miR-6825-5p gene" or "hsa-miR-6825-5p" is, hsa-miR-6825-5p gene (miRBase Accession set forth in SEQ ID NO: 16 No. MIMAT0027550) and other, including such species homologs or orthologs. hsa-miR-6825-5p gene can be obtained Ladewig E et al., 2012, Genome Res, 22 vol., by the method described in p1634-1645. Further, "hsa-miR-6825-5p" takes a hairpin-like structure "hsa-mir-6825" (miRBase Accession No.MI0022670, SEQ ID NO: 215) is known as its precursor.

[0088]

　The term as used herein, "hsa-miR-7108-5p gene" or "hsa-miR-7108-5p" is, hsa-miR-7108-5p gene (miRBase Accession described in SEQ ID NO. 17 No. MIMAT0028113) and other, including such species homologs or orthologs. hsa-miR-7108-5p gene can be obtained Ladewig E et al., 2012, Genome Res, 22 vol., by the method described in p1634-1645. Further, "hsa-miR-7108-5p" takes a hairpin-like structure "hsa-mir-7108" (miRBase Accession No.MI0022959, SEQ ID NO: 216) is known as its precursor.

[0089]

　The term as used herein, "hsa-miR-4792 gene" or "hsa-miR-4792" is, hsa-miR-4792 gene described in SEQ ID NO: 18 (miRBase Accession No.MIMAT0019964) and other species It encompasses such homologs or orthologs. hsa-miR-4792 gene can be obtained Persson H et al., 2011,. Cancer Res, 71 vol., by the method described in p78-86. Further, "hsa-miR-4792" takes a hairpin-like structure "hsa-mir-4792" (miRBase Accession No.MI0017439, SEQ ID NO: 217) is known as its precursor.

[0090]

　The term as used herein, "hsa-miR-7641 gene" or "hsa-miR-7641" is, hsa-miR-7641 gene described in SEQ ID NO: 19 (miRBase Accession No.MIMAT0029782) and other species It encompasses such homologs or orthologs. hsa-miR-7641 gene, Yoo JK et al., 2013, Arch Pharm Res, 36 vol., can be obtained by the method described in p353-358. In addition, "hsa-miR-7641" takes a hairpin-like structure as its precursor "hsa-mir-7641-1, hsa-mir-7641-2" (miRBase Accession No.MI0024975, MI0024976, SEQ ID NO: 218, 219) is known.

[0091]

　The term as used herein, "hsa-miR-3188 gene" or "hsa-miR-3188" is, hsa-miR-3188 gene described in SEQ ID NO: 20 (miRBase Accession No.MIMAT0015070) and other species It encompasses such homologs or orthologs. hsa-miR-3188 gene, Stark MS, et al., 2010, PLoS One, 5 volumes, can be obtained by the methods described in the e9685. Further, "hsa-miR-3188" takes a hairpin-like structure "hsa-mir-3188" (miRBase Accession No.MI0014232, SEQ ID NO: 220) is known as its precursor.

[0092]

　The term as used herein, "hsa-miR-3131 gene" or "hsa-miR-3131" is, hsa-miR-3131 gene described in SEQ ID NO: 21 (miRBase Accession No.MIMAT0014996) and other species It encompasses such homologs or orthologs. hsa-miR-3131 gene, Stark MS, et al., 2010, PLoS One, 5 volumes, can be obtained by the methods described in the e9685. Further, "hsa-miR-3131" takes a hairpin-like structure "hsa-mir-3131" (miRBase Accession No.MI0014151, SEQ ID NO: 221) is known as its precursor.

[0093]

　The term as used herein, "hsa-miR-6780b-5p gene" or "hsa-miR-6780b-5p" is, hsa-miR-6780b-5p gene described in SEQ ID NO: 22 (miRBase Accession No. MIMAT0027572) and other, including such species homologs or orthologs. hsa-miR-6780b-5p gene can be obtained Ladewig E et al., 2012, Genome Res, 22 vol., by the method described in p1634-1645. Further, "hsa-miR-6780b-5p" takes a hairpin-like structure "hsa-mir-6780b" (miRBase Accession No.MI0022681, SEQ ID NO: 222) is known as its precursor.

[0094]

　The term as used herein, "hsa-miR-8069 gene" or "hsa-miR-8069" is, hsa-miR-8069 gene described in SEQ ID NO: 23 (miRBase Accession No.MIMAT0030996) and other species It encompasses such homologs or orthologs. hsa-miR-8069 gene, Wang HJ, et al., 2013, Shock, 39 vol, may be obtained by the method described in p480-487. Further, "hsa-miR-8069" takes a hairpin-like structure "hsa-mir-8069" (miRBase Accession No.MI0025905, SEQ ID NO: 223) is known as its precursor.

[0095]

　The term as used herein, "hsa-miR-6840-3p gene" or "hsa-miR-6840-3p" is, hsa-miR-6840-3p gene (miRBase Accession described in SEQ ID NO: 24 No. MIMAT0027583) and other, including such species homologs or orthologs. hsa-miR-6840-3p gene can be obtained Ladewig E et al., 2012, Genome Res, 22 vol., by the method described in p1634-1645. Further, "hsa-miR-6840-3p" takes a hairpin-like structure "hsa-mir-6840" (miRBase Accession No.MI0022686, SEQ ID NO: 224) is known as its precursor.

[0096]

　The term as used herein, "hsa-miR-8072 gene" or "hsa-miR-8072" is, hsa-miR-8072 gene described in SEQ ID NO: 25 (miRBase Accession No.MIMAT0030999) and other species It encompasses such homologs or orthologs. hsa-miR-8072 gene, Wang HJ, et al., 2013, Shock, 39 vol, may be obtained by the method described in p480-487. Further, "hsa-miR-8072" takes a hairpin-like structure "hsa-mir-8072" (miRBase Accession No.MI0025908, SEQ ID NO: 225) is known as its precursor.

[0097]

　The term as used herein, "hsa-miR-1233-5p gene" or "hsa-miR-1233-5p" is, hsa-miR-1233-5p gene (miRBase Accession described in SEQ ID NO: 26 No. MIMAT0022943) and other, including such species homologs or orthologs. hsa-miR-1233-5p gene, Berezikov E et al., 2007, MoI the Cell, 28 vol, may be obtained by the method described in p328-336. In addition, "hsa-miR-1233-5p" takes a hairpin-like structure as its precursor "hsa-mir-1233-1, hsa-mir-1233-2" (miRBase Accession No.MI0006323, MI0015973, SEQ ID NO: 226 and 227) are known.

[0098]

　The term as used herein, "hsa-miR-6887-5p gene" or "hsa-miR-6887-5p" is, hsa-miR-6887-5p gene (miRBase Accession described in SEQ ID NO: 27 No. MIMAT0027674) and other, including such species homologs or orthologs. hsa-miR-6887-5p gene can be obtained Ladewig E et al., 2012, Genome Res, 22 vol., by the method described in p1634-1645. Further, "hsa-miR-6887-5p" takes a hairpin-like structure "hsa-mir-6887" (miRBase Accession No.MI0022734, SEQ ID NO: 228) is known as its precursor.

[0099]

　The term as used herein, "hsa-miR-1231 gene" or "hsa-miR-1231" is, hsa-miR-1231 gene described in SEQ ID NO: 28 (miRBase Accession No.MIMAT0005586) and other species It encompasses such homologs or orthologs. hsa-miR-1231 gene, Berezikov E et al., 2007, MoI the Cell, 28 vol, may be obtained by the method described in p328-336. Further, "hsa-miR-1231" takes a hairpin-like structure "hsa-mir-1231" (miRBase Accession No.MI0006321, SEQ ID NO: 229) is known as its precursor.

[0100]

　The term as used herein, "hsa-miR-5572 gene" or "hsa-miR-5572" is, hsa-miR-5572 gene described in SEQ ID NO: 29 (miRBase Accession No.MIMAT0022260) and other species It encompasses such homologs or orthologs. hsa-miR-5572 gene can be obtained Tandon M et al., 2012, Oral Dis, 18 vol., by the method described in p127-131. Further, "hsa-miR-5572" takes a hairpin-like structure "hsa-mir-5572" (miRBase Accession No.MI0019117, SEQ ID NO: 230) is known as its precursor.

[0101]

　The term as used herein, "hsa-miR-6738-5p gene" or "hsa-miR-6738-5p" is, hsa-miR-6738-5p gene (miRBase Accession described in SEQ ID NO: 30 No. MIMAT0027377) and other, including such species homologs or orthologs. hsa-miR-6738-5p gene can be obtained Ladewig E et al., 2012, Genome Res, 22 vol., by the method described in p1634-1645. Further, "hsa-miR-6738-5p" takes a hairpin-like structure "hsa-mir-6738" (miRBase Accession No.MI0022583, SEQ ID NO: 231) is known as its precursor.

[0102]

　The term as used herein, "hsa-miR-6784-5p gene" or "hsa-miR-6784-5p" is, hsa-miR-6784-5p gene (miRBase Accession described in SEQ ID NO: 31 No. MIMAT0027468) and other, including such species homologs or orthologs. hsa-miR-6784-5p gene can be obtained Ladewig E et al., 2012, Genome Res, 22 vol., by the method described in p1634-1645. Further, "hsa-miR-6784-5p" takes a hairpin-like structure "hsa-mir-6784" (miRBase Accession No.MI0022629, SEQ ID NO: 232) is known as its precursor.

The scope of the claims

[Claim 1]

　胃 が ん マ ー カ ー で あ る, miR-4257, miR-6726-5p, miR-1343-3p, miR-1247-3p, miR-6787-5p, miR-6875-5p, miR-1225-3p, miR-8063, miR -6781-5p, miR-4746-3p, miR-1908-5p, miR-6756-5p, miR-204-3p, miR-4651, miR-6757-5p, miR-6825-5p, miR-7108-5p , miR-4792, miR-7641, miR-3188, miR-3131, miR-6780b-5p, miR-8069, miR-6840-3p, miR-8072, miR-1233-5p, miR-6887-5p, miR -1231, miR-5572, miR-6738-5p, miR-6784-5p, miR-6791-5p, miR-6749-5p, miR-6741-5p, miR-128-1-5p, miR-4419b, miR -6746-5p, miR-3184-5p, miR-3679-5p, miR-7110-5p, miR-4516, miR-6717-5p, miR-6826-5p, miR-4433b-3p, miR-3679-3p , miR-3135b, miR-3622a-5p, miR-711, miR-4467, miR-6857-5p, miR-6515-3p, miR-1225-5p, miR-187-5p, miR-3185, miR-642b 3P, miR-1249, miR-744-5p, miR-4442, miR-1228-3p, miR-939-5p, miR-6845-5p, miR-887-3p, miR-7845-5p, miR-6729 -5p, miR-4632-5p, miR-615-5p, miR-6724-5p, miR-4728-5p, miR-6732-5p, miR-6816-5p, miR-4695-5p, miR-6088, miR -7975, miR-3197, miR-6125, miR-4433-3p, miR-6727-5p, miR-4706, miR-7847-3p, miR-6805-3p, miR-6766-3p, miR-1913, miR -4649-5p, miR-602, miR-3663-3p, miR-6893-5p, miR-6861-5p, miR-4449, miR-6842-5p, miR-4454, miR-5195-3p, miR-663b , miR-6765-5p, miR-4513, miR-614, miR-6785-5p, miR-6777-5p, miR-940, miR-4741, miR-6870-5p, miR-6131, miR-150-3p , miR-4707-5p, miR-1915-3p, miR-3937, miR-937-5p, miR-4443, miR-1914-3p, miR-3620-5p, miR-1268b, miR-1227-5p, miR -6880-5p, miR-4417, miR-6802-5p, miR-6769a-5p, miR-663a, miR-6721-5p, miR-4532, miR-7977, miR-92b-5p, miR-371a-5p , miR-6126, miR-4734, miR-4665-3p, miR-423-5p, miR-1469, miR-4675, miR-1915-5p, miR-6716-5p, miR-718, miR-4281, miR -6820-5p, miR-6795-5p, miR-6779-5p, miR-7109-5p, miR-6798-5p, miR-4648, miR-8059, miR-6765-3p, miR-6132, miR-4492 , miR-7107-5p, miR-3195, miR-3180, miR-296-3p, miR-564, miR-1268a, miR-6848-5p, miR-762, miR-2861, miR-1203, miR-1260b , miR-4476, miR-6885-5p, miR-6769b-5p, miR-23b-3p, miR-1343-5p, miR-3621, miR-4688, miR-4286, miR-4640-5p, miR-4739 , miR-1260a, miR-4276, miR-7106-5p, miR-6794-5p, miR-6774-5p, miR-4707-3p, miR-4534, miR-4294, miR-6850-5p, miR-6089及 び miR-671-5p か ら な る 群 か ら 選 択 さ れ る 少 な く と も 1 つ 以上 の ポ リ ヌ ク レ オ チ ド と 特異 的 に 結合 可能 な 核酸 を 含 む, 胃 が ん の 検 出 用 キ ッ ト.

[Claim 2]

　miR-4257 is hsa-miR-4257, miR-6726-5p is hsa-miR-6726-5p, miR-1343-3p is hsa-miR-1343-3p, the miR-1247-3p is hsa-miR-1247-3p, miR-6787-5p is hsa-miR-6787-5p, miR-6875-5p is hsa-miR-6875-5p, the miR-1225-3p hsa- is a miR-1225-3p, miR-8063 is hsa-miR-8063, miR-6781-5p is hsa-miR-6781-5p, miR-4746-3p is in hsa-miR-4746-3p Yes, miR-1908-5p is hsa-miR-1908-5p, miR-6756-5p is hsa-miR-6756-5p, miR-204-3p is hsa-miR-204-3p, miR-4651 is hsa-miR-4651, miR-6757-5p is hsa-miR-6757-5p, miR-6825-5p is hsa-miR-6825-5p, the miR-7108-5p is hsa-miR-7108-5p, miR-4792 is hsa-miR-4792, miR-7641 is hsa-miR-7641, miR-3188 is hsa-miR-3188, is miR-3131 is hsa-miR-3131, miR-6780b-5p is hsa-miR-6780b-5p, miR-8069 is hsa-miR-8069, miR-6840-3p is in hsa-miR-6840-3p Yes, miR-8072 is hsa-miR-8072, miR-1233-5p is hsa-miR-1233-5p, miR-6887-5p is hsa-miR-6887-5p, miR-1231 is is hsa-miR-1231, miR-5572 is hsa-miR-5572, miR-6738-5p is hsa-miR-6738-5p, miR-6784-5p is in hsa-miR-6784-5p Yes, miR-6791-5p is hsa-miR-6791-5p, miR-6749-5p is hsa-miR-6749-5p, miR-6741-5p is hsa-miR-6741-5p, miR-128-1-5p is hsa-miR-128-1-5p, miR-4419b is hsa-miR-4419b, miR-6746-5p is hsa-miR-6746-5p, miR- 3184-5p is hsa-miR-3184-5p, miR-3679-5p is hsa-miR-3679-5p, miR-7110-5p is hsa-miR-7110-5p, the miR-4516 is hsa-miR-4516, miR-6717-5p is hsa-miR-6717-5p, miR-6826-5p is hsa-miR-6826-5p, miR-4433b-3p is hsa-miR- a 4433b-3p, miR-3679-3p is hsa-miR-3679-3p, miR-3135b is hsa-miR-3135b, miR-3622a-5p is hsa-miR-3622a-5p, miR-711 is hsa-miR-711, miR-4467 is hsa-miR-4467, miR-6857-5p is hsa-miR-6857-5p, miR-6515-3p is hsa-miR- a 6515-3p, miR-1225-5p is hsa-miR-1225-5p, miR-187-5p is hsa-miR-187-5p, miR-3185 is hsa-miR-3185, miR-642b-3p is hsa-miR-642b-3p, miR-1249 is hsa-miR-1249, miR-744-5p is hsa-miR-744-5p, miR-4442 is hsa- is a miR-4442, miR-1228-3p is hsa-miR-1228-3p, miR-939-5p is hsa-miR-939-5p, miR-6845-5p is hsa-miR-6845- a 5p, miR-887-3p is hsa-miR-887-3p, miR-7845-5p is hsa-miR-7845-5p, miR-6729-5p is in hsa-miR-6729-5p Yes, miR-4632-5p is hsa-miR-4632-5p, miR-615-5p is hsa-miR-615-5p, miR-6724-5p is hsa-miR-6724-5p, miR-4728-5p is hsa-miR-4728-5p, miR-6732-5p is hsa-miR-6732-5p, miR-6816-5p is hsa-miR-6816-5p, miR- 4695-5p is hsa-miR-4695-5p, miR-6088 is hsa-miR-6088, miR-7975 is hsa-miR-7975, miR-3197 is hsa-miR-3197, miR-6125 is hsa-miR-6125, miR-4433-3p is hsa-miR-4433-3p, miR-6727-5p is hsa-miR-6727-5p, miR-4706 is hsa- is a miR-4706, miR-7847-3p is hsa-miR-7847-3p, miR-6805-3p is hsa-miR-6805-3p, miR-6766-3p is hsa-miR-6766- is 3p, miR-1913 is hsa-miR-1913, miR-4649-5p is hsa-miR-4649-5p, miR-602 is hsa-miR-602, is miR-3663-3p is hsa-miR-3663-3p, miR-6893-5p is hsa-miR-6893-5p, miR-6861-5p is hsa-miR-6861-5p, miR-4449 is hsa-miR- is 4449, miR-6842-5p is hsa-miR-6842-5p, miR-4454 is hsa-miR-4454, miR-5195-3p is hsa-miR-5195-3p, miR- 663b is hsa-miR-663b, miR-6765-5p is hsa-miR-6765-5p, miR-4513 is hsa-miR-4513, miR-614 is hsa-miR-614, miR-6785-5p is hsa-miR-6785-5p, miR-6777-5p is hsa-miR-6777-5p, miR-940 is hsa-miR-940, miR-4741 is hsa- is a miR-4741, miR-6870-5p is hsa-miR-6870-5p, miR-6131 is hsa-miR-6131, miR-150-3p is hsa-miR-150-3p, miR-4707-5p is hsa-miR-4707-5p, miR-1915-3p is hsa-miR-1915-3p, miR-3937 is hsa-miR-3937, is miR-937-5p is hsa-miR-937-5p, miR-4443 is hsa-miR-4443, miR-1914-3p is hsa-miR-1914-3p, miR-3620-5p is hsa-miR-3620- a 5p, miR-1268b is hsa-miR-1268b, miR-1227-5p is hsa-miR-1227-5p, miR-6880-5p is hsa-miR-6880-5p, miR- 4417 is hsa-miR-4417, miR-6802-5p is hsa-miR-6802-5p, miR-6769a-5p is hsa-miR-6769a-5p, miR-663a is hsa-miR- a 663a, miR-6721-5p is hsa-miR-6721-5p, miR-4532 is hsa-miR-4532, miR-7977 is hsa-miR-7977, is miR-92b-5p is hsa-miR-92b-5p, miR-371a-5p is hsa-miR-371a-5p, miR-6126 is hsa-miR-6126, miR-4734 is hsa-miR-4734, miR-4665-3p is hsa-miR-4665-3p, miR-423-5p is hsa-miR-423-5p, miR-1469 is hsa-miR-1469, miR-4675 is hsa- is a miR-4675, miR-1915-5p is hsa-miR-1915-5p, miR-6716-5p is hsa-miR-6716-5p, miR-718 is hsa-miR-718, miR-4281 is hsa-miR-4281, miR-6820-5p is hsa-miR-6820-5p, miR-6795-5p is hsa-miR-6795-5p, the miR-6779-5p is hsa-miR-6779-5p, miR-7109-5p is hsa-miR-7109-5p, miR-6798-5p is hsa-miR-6798-5p, miR-4648 is hsa-miR- is 4648, miR-8059 is hsa-miR-8059, miR-6765-3p is hsa-miR-6765-3p, miR-6132 is hsa-miR-6132, miR-4492 is hsa- is a miR-4492, miR-7107-5p is hsa-miR-7107-5p, miR-3195 is hsa-miR-3195, miR-3180 is hsa-miR-3180, miR-296- 3p is hsa-miR-296-3p, miR-564 is hsa-miR-564, miR-1268a is hsa-miR-1268a, miR-6848-5p is in hsa-miR-6848-5p Yes, miR-762 is hsa-miR-762, miR-2861 is hsa-miR-2861, miR-1203 is hsa-miR-1203, miR-1260b is hsa-miR-1260b, miR-4476 is hsa-miR-4476, miR-6885-5p is hsa-miR-6885-5p, miR-6769b-5p is hsa-miR-6769b-5p, the miR-23b-3p is hsa-miR-23b-3p, miR-1343-5p is hsa-miR-1343-5p, miR-3621 is hsa-miR-3621, miR-4688 is hsa-miR-4688, miR-4286 is hsa-miR-4286, miR-4640-5p is hsa-miR-4640-5p, miR-4739 is hsa-miR-4739, miR-1260a is in hsa-miR-1260a Yes, miR-4276 is hsa-miR-4276, miR-7106-5p is hsa-miR-7106-5p, miR-6794-5p is hsa-miR-6794-5p, miR-6774- 5p is hsa-miR-6774-5p, miR-4707-3p is hsa-miR-4707-3p, miR-4534 is hsa-miR-4534, miR-4294 is in the hsa-miR-4294 Yes, miR-6850-5p is hsa-miR-6850-5p, miR-6089 is hsa-miR-6089, and, miR-671-5p is hsa-miR-671-5p, claim the kit according to 1.

[Claim 3]

　Said nucleic acid, the following (a) ~ (e) to indicate a
polynucleotide: (a) a base u in the nucleotide sequence or the nucleotide sequence shown in any of SEQ ID NOs 165 and 635-642 is t a polynucleotide consisting of the sequence, a variant thereof, a derivative thereof, or a fragment comprising 15 or more continuous nucleotides,
polynucleotides comprising a nucleotide sequence represented by any one of (b) SEQ ID NOs 165 and 635-642 ,
polynucleotides, variants thereof u in the nucleotide sequence or the nucleotide sequence represented by any one of (c)
complementary to the nucleotide sequence u is t in the nucleotide sequence or the nucleotide sequence represented by any one of (d) SEQ ID NOs 165 and 635-642 polynucleotides comprising a nucleotide sequence, and
(e) above (a) ~ any polynucleotide under stringent conditions with a polynucleotide hybridizing of (d-),
a polynucleotide selected from the group consisting of, the kit according to claim 1 or 2.

[Claim 4]

　The kit is another gastric cancer marker, miR-128-2-5p, miR-125a-3p, at least one or more selected from the group consisting of miR-92a-2-5p and miR-486-3p further comprising a polynucleotide capable of specifically binding nucleic acids, the kit according to any one of claims 1 to 3.

[Claim 5]

　miR-128-2-5p is hsa-miR-128-2-5p, miR-125a-3p is hsa-miR-125a-3p, miR-92a-2-5p is hsa-miR-92a- it is 2-5p, and, miR-486-3p is hsa-miR-486-3p, kit according to claim 4.

[6.]

　It said nucleic acid, the following (f) ~ (j) are shown polynucleotide:
(f) Poly u in the nucleotide sequence or the nucleotide sequence represented by any of SEQ ID NO: 166-169 are the nucleotide sequence is t nucleotides, a variant thereof, a derivative thereof, or a fragment comprising 15 or more continuous
nucleotides, polynucleotides comprising a nucleotide sequence represented by any one of (g) SEQ ID NO: 166 -
169, (h) SEQ ID NO: 166 polynucleotides u in the nucleotide sequence or the nucleotide sequence represented by any one of 169 consisting of a nucleotide sequence complementary to the nucleotide sequence which is t, a variant thereof, the derivatives thereof, or 15 or more contiguous bases fragment,
(i) SEQ ID NO: 166 polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence u is t in the nucleotide sequence or the nucleotide sequence represented by any one of 169, and
(j) wherein (f) any polynucleotide under stringent conditions with a polynucleotide hybridizing of ~ (i),
a polynucleotide selected from the group consisting of a kit according to claim 4 or 5.

[7.]

　The kit is another of gastric cancer marker, miR-3196, miR-211-3p, miR-4271, miR-6851-5p, miR-149-3p, miR-4667-5p, miR-135a-3p, miR -4486, miR-4697-5p, miR-4725-3p, miR-6510-5p, miR-5001-5p, miR-4673, miR-4466, miR-23a-3p, miR-3656, miR-6782-5p , miR-4689, miR-451a, miR-4446-3p, miR-3180-3p, miR-642a-3p, miR-6889-5p, miR-3178, miR-4665-5p, miR-6722-3p, miR -30c-1-3p, further comprising a miR-4507, miR-3141 and at least one polynucleotide capable of specifically binding nucleic acid is selected from the group consisting of miR-1199-5p, claim 1 6 any one kit according to any one of the.

[8.]

　miR-3196 is hsa-miR-3196, miR-211-3p is hsa-miR-211-3p, miR-4271 is hsa-miR-4271, miR-6851-5p is hsa-miR- a 6851-5p, miR-149-3p is hsa-miR-149-3p, miR-4667-5p is hsa-miR-4667-5p, miR-135a-3p is hsa-miR-135a- is 3p, miR-4486 is hsa-miR-4486, miR-4697-5p is hsa-miR-4697-5p, miR-4725-3p is hsa-miR-4725-3p, miR- 6510-5p is hsa-miR-6510-5p, miR-5001-5p is hsa-miR-5001-5p, miR-4673 is hsa-miR-4673, miR-4466 is hsa-miR- is 4466, miR-23a-3p is hsa-miR-23a-3p, miR-3656 is hsa-miR-3656, miR-6782-5p is hsa-miR-6782-5p, miR- 4689 is hsa-miR-4689, miR-451a is hsa-miR-451a, miR-4446-3p is hsa-miR-4446-3p, miR-3180-3p is hsa-miR-3180- is 3p, miR-642a-3p is hsa-miR-642a-3p, miR-6889-5p is hsa-miR-6889-5p, miR-3178 is hsa-miR-3178, miR- 4665-5p is hsa-miR-4665-5p, miR-6722-3p is hsa-miR-6722-3p, miR-30c-1-3p is hsa-miR-30c-1-3p, miR-4507 is hsa-miR-4507, miR-3141 is hsa-miR-3141, and, miR-1199-5p is hsa-miR-1199-5p, kit according to claim 7.

[9.]

　It said nucleic acid, the following (k) a polynucleotide shown in ~ (o):
(k) Poly u in the nucleotide sequence or the nucleotide sequence represented by any of SEQ ID NO: 170-199 are the nucleotide sequence is t nucleotides, a variant thereof, a derivative thereof, or a fragment comprising 15 or more continuous
nucleotides, polynucleotides comprising a nucleotide sequence represented by any of (l) SEQ ID NO: 170 to
199, (yd) SEQ ID NO: 170 ~ polynucleotides u in the nucleotide sequence or the nucleotide sequence represented by any one of 199 consisting of a nucleotide sequence complementary to the nucleotide sequence which is t, a variant thereof, the derivatives thereof, or 15 or more contiguous bases fragment,
a polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence that u is t in the nucleotide sequence or the nucleotide sequence represented by any one of (n) SEQ ID NO: 170-199, and
(o) the (k) any polynucleotide under stringent conditions with a polynucleotide hybridizing of ~ (n),
is a polynucleotide selected from the group consisting of a kit according to claim 7 or 8.

[10.]

　The kit comprises at least two or more nucleic acids respectively capable of specifically binding at least two or more polynucleotides selected from all the gastric cancer marker according to claim 1 or 2, claims 1 to 9 the kit according to any one of.

[11.]

　胃 が ん マ ー カ ー で あ る, miR-4257, miR-6726-5p, miR-1343-3p, miR-1247-3p, miR-6787-5p, miR-6875-5p, miR-1225-3p, miR-8063, miR -6781-5p, miR-4746-3p, miR-1908-5p, miR-6756-5p, miR-204-3p, miR-4651, miR-6757-5p, miR-6825-5p, miR-7108-5p , miR-4792, miR-7641, miR-3188, miR-3131, miR-6780b-5p, miR-8069, miR-6840-3p, miR-8072, miR-1233-5p, miR-6887-5p, miR -1231, miR-5572, miR-6738-5p, miR-6784-5p, miR-6791-5p, miR-6749-5p, miR-6741-5p, miR-128-1-5p, miR-4419b, miR -6746-5p, miR-3184-5p, miR-3679-5p, miR-7110-5p, miR-4516, miR-6717-5p, miR-6826-5p, miR-4433b-3p, miR-3679-3p , miR-3135b, miR-3622a-5p, miR-711, miR-4467, miR-6857-5p, miR-6515-3p, miR-1225-5p, miR-187-5p, miR-3185, miR-642b 3P, miR-1249, miR-744-5p, miR-4442, miR-1228-3p, miR-939-5p, miR-6845-5p, miR-887-3p, miR-7845-5p, miR-6729 -5p, miR-4632-5p, miR-615-5p, miR-6724-5p, miR-4728-5p, miR-6732-5p, miR-6816-5p, miR-4695-5p, miR-6088, miR -7975, miR-3197, miR-6125, miR-4433-3p, miR-6727-5p, miR-4706, miR-7847-3p, miR-6805-3p, miR-6766-3p, miR-1913, miR -4649-5p, miR-602, miR-3663-3p, miR-6893-5p, miR-6861-5p, miR-4449, miR-6842-5p, miR-4454, miR-5195-3p, miR-663b , miR-6765-5p, miR-4513, miR-614, miR-6785-5p, miR-6777-5p, miR-940, miR-4741, miR-6870-5p, miR-6131, miR-150-3p , miR-4707-5p, miR-1915-3p, miR-3937, miR-937-5p, miR-4443, miR-1914-3p, miR-3620-5p, miR-1268b, miR-1227-5p, miR -6880-5p, miR-4417, miR-6802-5p, miR-6769a-5p, miR-663a, miR-6721-5p, miR-4532, miR-7977, miR-92b-5p, miR-371a-5p , miR-6126, miR-4734, miR-4665-3p, miR-423-5p, miR-1469, miR-4675, miR-1915-5p, miR-6716-5p, miR-718, miR-4281, miR -6820-5p, miR-6795-5p, miR-6779-5p, miR-7109-5p, miR-6798-5p, miR-4648, miR-8059, miR-6765-3p, miR-6132, miR-4492 , miR-7107-5p, miR-3195, miR-3180, miR-296-3p, miR-564, miR-1268a, miR-6848-5p, miR-762, miR-2861, miR-1203, miR-1260b , miR-4476, miR-6885-5p, miR-6769b-5p, miR-23b-3p, miR-1343-5p, miR-3621, miR-4688, miR-4286, miR-4640-5p, miR-4739 , miR-1260a, miR-4276, miR-7106-5p, miR-6794-5p, miR-6774-5p, miR-4707-3p, miR-4534, miR-4294, miR-6850-5p, miR-6089及 び miR-671-5p か ら な る 群 か ら 選 択 さ れ る 少 な く と も 1 つ 以上 の ポ リ ヌ ク レ オ チ ド と 特異 的 に 結合 可能 な 核酸 を 含 む, 胃 が ん の 検 出 用 デ バ イ ス.

[12.]

　miR-4257 is hsa-miR-4257, miR-6726-5p is hsa-miR-6726-5p, miR-1343-3p is hsa-miR-1343-3p, the miR-1247-3p is hsa-miR-1247-3p, miR-6787-5p is hsa-miR-6787-5p, miR-6875-5p is hsa-miR-6875-5p, the miR-1225-3p hsa- is a miR-1225-3p, miR-8063 is hsa-miR-8063, miR-6781-5p is hsa-miR-6781-5p, miR-4746-3p is in hsa-miR-4746-3p Yes, miR-1908-5p is hsa-miR-1908-5p, miR-6756-5p is hsa-miR-6756-5p, miR-204-3p is hsa-miR-204-3p, miR-4651 is hsa-miR-4651, miR-6757-5p is hsa-miR-6757-5p, miR-6825-5p is hsa-miR-6825-5p, the miR-7108-5p is hsa-miR-7108-5p, miR-4792 is hsa-miR-4792, miR-7641 is hsa-miR-7641, miR-3188 is hsa-miR-3188, is miR-3131 is hsa-miR-3131, miR-6780b-5p is hsa-miR-6780b-5p, miR-8069 is hsa-miR-8069, miR-6840-3p is in hsa-miR-6840-3p Yes, miR-8072 is hsa-miR-8072, miR-1233-5p is hsa-miR-1233-5p, miR-6887-5p is hsa-miR-6887-5p, miR-1231 is is hsa-miR-1231, miR-5572 is hsa-miR-5572, miR-6738-5p is hsa-miR-6738-5p, miR-6784-5p is in hsa-miR-6784-5p Yes, miR-6791-5p is hsa-miR-6791-5p, miR-6749-5p is hsa-miR-6749-5p, miR-6741-5p is hsa-miR-6741-5p, miR-128-1-5p is hsa-miR-128-1-5p, miR-4419b is hsa-miR-4419b, miR-6746-5p is hsa-miR-6746-5p, miR- 3184-5p is hsa-miR-3184-5p, miR-3679-5p is hsa-miR-3679-5p, miR-7110-5p is hsa-miR-7110-5p, the miR-4516 is hsa-miR-4516, miR-6717-5p is hsa-miR-6717-5p, miR-6826-5p is hsa-miR-6826-5p, miR-4433b-3p is hsa-miR- a 4433b-3p, miR-3679-3p is hsa-miR-3679-3p, miR-3135b is hsa-miR-3135b, miR-3622a-5p is hsa-miR-3622a-5p, miR-711 is hsa-miR-711, miR-4467 is hsa-miR-4467, miR-6857-5p is hsa-miR-6857-5p, miR-6515-3p is hsa-miR- a 6515-3p, miR-1225-5p is hsa-miR-1225-5p, miR-187-5p is hsa-miR-187-5p, miR-3185 is hsa-miR-3185, miR-642b-3p is hsa-miR-642b-3p, miR-1249 is hsa-miR-1249, miR-744-5p is hsa-miR-744-5p, miR-4442 is hsa- is a miR-4442, miR-1228-3p is hsa-miR-1228-3p, miR-939-5p is hsa-miR-939-5p, miR-6845-5p is hsa-miR-6845- a 5p, miR-887-3p is hsa-miR-887-3p, miR-7845-5p is hsa-miR-7845-5p, miR-6729-5p is in hsa-miR-6729-5p Yes, miR-4632-5p is hsa-miR-4632-5p, miR-615-5p is hsa-miR-615-5p, miR-6724-5p is hsa-miR-6724-5p, miR-4728-5p is hsa-miR-4728-5p, miR-6732-5p is hsa-miR-6732-5p, miR-6816-5p is hsa-miR-6816-5p, miR- 4695-5p is hsa-miR-4695-5p, miR-6088 is hsa-miR-6088, miR-7975 is hsa-miR-7975, miR-3197 is hsa-miR-3197, miR-6125 is hsa-miR-6125, miR-4433-3p is hsa-miR-4433-3p, miR-6727-5p is hsa-miR-6727-5p, miR-4706 is hsa- is a miR-4706, miR-7847-3p is hsa-miR-7847-3p, miR-6805-3p is hsa-miR-6805-3p, miR-6766-3p is hsa-miR-6766- is 3p, miR-1913 is hsa-miR-1913, miR-4649-5p is hsa-miR-4649-5p, miR-602 is hsa-miR-602, is miR-3663-3p is hsa-miR-3663-3p, miR-6893-5p is hsa-miR-6893-5p, miR-6861-5p is hsa-miR-6861-5p, miR-4449 is hsa-miR- is 4449, miR-6842-5p is hsa-miR-6842-5p, miR-4454 is hsa-miR-4454, miR-5195-3p is hsa-miR-5195-3p, miR- 663b is hsa-miR-663b, miR-6765-5p is hsa-miR-6765-5p, miR-4513 is hsa-miR-4513, miR-614 is hsa-miR-614, miR-6785-5p is hsa-miR-6785-5p, miR-6777-5p is hsa-miR-6777-5p, miR-940 is hsa-miR-940, miR-4741 is hsa- is a miR-4741, miR-6870-5p is hsa-miR-6870-5p, miR-6131 is hsa-miR-6131, miR-150-3p is hsa-miR-150-3p, miR-4707-5p is hsa-miR-4707-5p, miR-1915-3p is hsa-miR-1915-3p, miR-3937 is hsa-miR-3937, is miR-937-5p is hsa-miR-937-5p, miR-4443 is hsa-miR-4443, miR-1914-3p is hsa-miR-1914-3p, miR-3620-5p is hsa-miR-3620- a 5p, miR-1268b is hsa-miR-1268b, miR-1227-5p is hsa-miR-1227-5p, miR-6880-5p is hsa-miR-6880-5p, miR- 4417 is hsa-miR-4417, miR-6802-5p is hsa-miR-6802-5p, miR-6769a-5p is hsa-miR-6769a-5p, miR-663a is hsa-miR- a 663a, miR-6721-5p is hsa-miR-6721-5p, miR-4532 is hsa-miR-4532, miR-7977 is hsa-miR-7977, is miR-92b-5p is hsa-miR-92b-5p, miR-371a-5p is hsa-miR-371a-5p, miR-6126 is hsa-miR-6126, miR-4734 is hsa-miR-4734, miR-4665-3p is hsa-miR-4665-3p, miR-423-5p is hsa-miR-423-5p, miR-1469 is hsa-miR-1469, miR-4675 is hsa- is a miR-4675, miR-1915-5p is hsa-miR-1915-5p, miR-6716-5p is hsa-miR-6716-5p, miR-718 is hsa-miR-718, miR-4281 is hsa-miR-4281, miR-6820-5p is hsa-miR-6820-5p, miR-6795-5p is hsa-miR-6795-5p, the miR-6779-5p is hsa-miR-6779-5p, miR-7109-5p is hsa-miR-7109-5p, miR-6798-5p is hsa-miR-6798-5p, miR-4648 is hsa-miR- is 4648, miR-8059 is hsa-miR-8059, miR-6765-3p is hsa-miR-6765-3p, miR-6132 is hsa-miR-6132, miR-4492 is hsa- is a miR-4492, miR-7107-5p is hsa-miR-7107-5p, miR-3195 is hsa-miR-3195, miR-3180 is hsa-miR-3180, miR-296- 3p is hsa-miR-296-3p, miR-564 is hsa-miR-564, miR-1268a is hsa-miR-1268a, miR-6848-5p is in hsa-miR-6848-5p Yes, miR-762 is hsa-miR-762, miR-2861 is hsa-miR-2861, miR-1203 is hsa-miR-1203, miR-1260b is hsa-miR-1260b, miR-4476 is hsa-miR-4476, miR-6885-5p is hsa-miR-6885-5p, miR-6769b-5p is hsa-miR-6769b-5p, the miR-23b-3p is hsa-miR-23b-3p, miR-1343-5p is hsa-miR-1343-5p, miR-3621 is hsa-miR-3621, miR-4688 is hsa-miR-4688, miR-4286 is hsa-miR-4286, miR-4640-5p is hsa-miR-4640-5p, miR-4739 is hsa-miR-4739, miR-1260a is in hsa-miR-1260a Yes, miR-4276 is hsa-miR-4276, miR-7106-5p is hsa-miR-7106-5p, miR-6794-5p is hsa-miR-6794-5p, miR-6774- 5p is hsa-miR-6774-5p, miR-4707-3p is hsa-miR-4707-3p, miR-4534 is hsa-miR-4534, miR-4294 is in the hsa-miR-4294 Yes, miR-6850-5p is hsa-miR-6850-5p, miR-6089 is hsa-miR-6089, and, miR-671-5p is hsa-miR-671-5p, claim a device according to 11.

[13.]

　Said nucleic acid, the following (a) ~ (e) to indicate a
polynucleotide: (a) a base u in the nucleotide sequence or the nucleotide sequence shown in any of SEQ ID NOs 165 and 635-642 is t a polynucleotide consisting of the sequence, a variant thereof, a derivative thereof, or a fragment comprising 15 or more continuous nucleotides,
polynucleotides comprising a nucleotide sequence represented by any one of (b) SEQ ID NOs 165 and 635-642 ,
polynucleotides, variants thereof u in the nucleotide sequence or the nucleotide sequence represented by any one of (c)
complementary to the nucleotide sequence u is t in the nucleotide sequence or the nucleotide sequence represented by any one of (d) SEQ ID NOs 165 and 635-642 polynucleotides comprising a nucleotide sequence, and
(e) above (a) ~ any polynucleotide under stringent conditions with a polynucleotide hybridizing of (d-),
a polynucleotide selected from the group consisting of, device according to claim 11 or 12.

[14.]

　It said device is another gastric cancer marker, miR-128-2-5p, miR-125a-3p, at least one or more selected from the group consisting of miR-92a-2-5p and miR-486-3p further comprising a polynucleotide capable of specifically binding nucleic acids, a device according to any one of claims 11 to 13.

[15.]

　miR-128-2-5p is hsa-miR-128-2-5p, miR-125a-3p is hsa-miR-125a-3p, miR-92a-2-5p is hsa-miR-92a- it is 2-5p, and, miR-486-3p is hsa-miR-486-3p, device according to claim 14.

[16.]

　It said nucleic acid, the following (f) ~ (j) are shown polynucleotide:
(f) Poly u in the nucleotide sequence or the nucleotide sequence represented by any of SEQ ID NO: 166-169 are the nucleotide sequence is t nucleotides, a variant thereof, a derivative thereof, or a fragment comprising 15 or more continuous
nucleotides, polynucleotides comprising a nucleotide sequence represented by any one of (g) SEQ ID NO: 166 -
169, (h) SEQ ID NO: 166 polynucleotides u in the nucleotide sequence or the nucleotide sequence represented by any one of 169 consisting of a nucleotide sequence complementary to the nucleotide sequence which is t, a variant thereof, the derivatives thereof, or 15 or more contiguous bases fragment,
(i) SEQ ID NO: 166 polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence u is t in the nucleotide sequence or the nucleotide sequence represented by any one of 169, and
(j) wherein (f) any polynucleotide under stringent conditions with a polynucleotide hybridizing of ~ (i),
a polynucleotide selected from the group consisting of a device according to claim 14 or 15.

[17.]

　The device is another of gastric cancer marker, miR-3196, miR-211-3p, miR-4271, miR-6851-5p, miR-149-3p, miR-4667-5p, miR-135a-3p, miR -4486, miR-4697-5p, miR-4725-3p, miR-6510-5p, miR-5001-5p, miR-4673, miR-4466, miR-23a-3p, miR-3656, miR-6782-5p , miR-4689, miR-451a, miR-4446-3p, miR-3180-3p, miR-642a-3p, miR-6889-5p, miR-3178, miR-4665-5p, miR-6722-3p, miR -30c-1-3p, further comprising a miR-4507, miR-3141 and at least one polynucleotide capable of specifically binding nucleic acid is selected from the group consisting of miR-1199-5p, claims 11 to 16 device according to any one of.

[18.]

　miR-3196 is hsa-miR-3196, miR-211-3p is hsa-miR-211-3p, miR-4271 is hsa-miR-4271, miR-6851-5p is hsa-miR- a 6851-5p, miR-149-3p is hsa-miR-149-3p, miR-4667-5p is hsa-miR-4667-5p, miR-135a-3p is hsa-miR-135a- is 3p, miR-4486 is hsa-miR-4486, miR-4697-5p is hsa-miR-4697-5p, miR-4725-3p is hsa-miR-4725-3p, miR- 6510-5p is hsa-miR-6510-5p, miR-5001-5p is hsa-miR-5001-5p, miR-4673 is hsa-miR-4673, miR-4466 is hsa-miR- is 4466, miR-23a-3p is hsa-miR-23a-3p, miR-3656 is hsa-miR-3656, miR-6782-5p is hsa-miR-6782-5p, miR- 4689 is hsa-miR-4689, miR-451a is hsa-miR-451a, miR-4446-3p is hsa-miR-4446-3p, miR-3180-3p is hsa-miR-3180- is 3p, miR-642a-3p is hsa-miR-642a-3p, miR-6889-5p is hsa-miR-6889-5p, miR-3178 is hsa-miR-3178, miR- 4665-5p is hsa-miR-4665-5p, miR-6722-3p is hsa-miR-6722-3p, miR-30c-1-3p is hsa-miR-30c-1-3p, miR-4507 is hsa-miR-4507, miR-3141 is hsa-miR-3141, and, miR-1199-5p is hsa-miR-1199-5p, according to claim 17, wherein the device.

[19.]

　It said nucleic acid, the following (k) a polynucleotide shown in ~ (o):
(k) Poly u in the nucleotide sequence or the nucleotide sequence represented by any of SEQ ID NO: 170-199 are the nucleotide sequence is t nucleotides, a variant thereof, a derivative thereof, or a fragment comprising 15 or more continuous
nucleotides, polynucleotides comprising a nucleotide sequence represented by any of (l) SEQ ID NO: 170 to
199, (yd) SEQ ID NO: 170 ~ polynucleotides u in the nucleotide sequence or the nucleotide sequence represented by any one of 199 consisting of a nucleotide sequence complementary to the nucleotide sequence which is t, a variant thereof, the derivatives thereof, or 15 or more contiguous bases fragment,
a polynucleotide comprising a nucleotide sequence complementary to the nucleotide sequence that u is t in the nucleotide sequence or the nucleotide sequence represented by any one of (n) SEQ ID NO: 170-199, and
(o) the (k) any polynucleotide under stringent conditions with a polynucleotide hybridizing of ~ (n),
is a polynucleotide selected from the group consisting of, a device according to claim 17 or 18.

[20.]

　It said device is a device for measuring by hybridization techniques, according to any one of claims 11 to 19 devices.

[21.]

　The hybridization techniques, a nucleic acid array technology, according to claim 20 devices.

[22.]

　The device comprises at least two or more nucleic acids respectively capable of specifically binding at least two or more polynucleotides selected from all the gastric cancer marker according to claim 11 or 12, claims 11-21 device according to any one of.

[23.]

　Using a device according to any one of claims 1 kit according to any one of 10 or claims 11 to 22 to measure the expression level of a target nucleic acid in the specimen of a subject, is the measurement and the expression level was, by using the control expression level in healthy body that is measured similarly, comprises the subject to be suffering from gastric cancer, or evaluated in vitro a not to be suffering from gastric cancer, detection method of gastric cancer.

[24.]

　The subject is a human The method of claim 23.

[25.]

　The sample is blood, serum or plasma Method according to claim 23 or 24.