Abstract: Abstract: This study uses molecular tools to characterize (he bacteria found in sewage samples and looks into their diversification. To determine the makeup ofthe microbial community, sewage samples were taken from surrounding localities and subjected to high-throughput sequencing. The findings showed that the sewage environment was home to a wide variety of microorganisms, including bacteria, fungus, viruses, and archaea‘ Additionally, particular microbial species were identified and their genetic diversity was evaluated through the use of molecular characterization techniques like DNA sequencing and polymerase chain reaction (PCR). To reduce potential health concerns and improve treatment procedures, it is essential to comprehend the biodiversity of microorganisms Found in sewage. This work emphasizes the significance of using molecular methods for thorough characterization and monitoring while also providing insightfizll information on 1110 microbial ecology ofsewagc settings. It is necessaly lo conduct Further study in this area to clarify the functions of particular bacteria in sewage ecosystems and their wider effects on environmental and human health. The results show how dynamic and complicated the microbial populations are in sewage systems, which has consequences for environmental management, waslcwaler treatment techniques, and public health. It is essential to comprehend the diversity of bacteria found in sewage.
Field of Invention:
The study on the biodiversity of microorganisms in sewage samples and molecular
characterization represents a pioneering field of invention with profound implications for
environmental monitoring and public health. This innovative approach aims to comprehensively
analyze the microbial composition ofsewage systems, shedding light on the intricate dynamics of
microbial communities therein. By employing culling-edge molecular techniques, such as highthroughput
sequencing and melagcnomic analysis, researchers can identify and characterize the
diverse array of microorganisms present in sewage samples at an unprecedented level of detail.
This includes bacteria, Viruses, fungi, and other microbial entities: providing insights into their
taxonomic diversity, functional potential, and ecological roles within the sewage ecosystem.
Moreover, molecular clmracterizalion techniques allow for the detection and monitoring ol‘spccific
microbial taxa, including potential pathogens and indicators of environmental contamination.
Through comprehensive bi“0diV€l‘Sily analysis and molecular profiling, this field ofinvcnlion offers
valuable tools for assessing the health and stabil‘ily of sewage systems, as well as for designing
targeted strategies for wastewater treatment and environmental management. Furthennore, 1he
findings generated through this innovative approach can inform evidence-based decision-making
in public health policy, urban planning, and environmental conservation effons.
Claims:
I. Through molecular characterization, microbiological components from sewage samples were
investigated and evaluated.
2.As mentioned in claim (I), the work is given as microbiological analysis using molecular
characterization tools such as gene sequencing and the required microbes are taken from In vitro
culture of microorganisms using the sewage sample.
3.As given in claim (2), the sewage sample was collected and the nutrient broth of [00 ml was
prepared in which the sample has been added to 50 ml and was incubated
4‘As given in claim (3) ,the agar medium was prepared and the bacterial culture was transferred to
the perri dish containing the media using spread plate technique.
5.As given in claim (4) The suimble bacterial colonies were selected and cultured again.
6.As mentioned in claim (I) The analysis is clone [0 (letennine the efficacy of the microbial strains
for beneficial applications
| # | Name | Date |
|---|---|---|
| 1 | 202441039252-Form 9-200524.pdf | 2024-05-22 |
| 2 | 202441039252-Form 5-200524.pdf | 2024-05-22 |
| 3 | 202441039252-Form 3-200524.pdf | 2024-05-22 |
| 4 | 202441039252-Form 2(Title Page)-200524.pdf | 2024-05-22 |
| 5 | 202441039252-Form 1-200524.pdf | 2024-05-22 |