FORM 2
THE PATENTS ACT, 1970 (Act 39 of 1970)
COMPLETE SPECIFICATION
(See Section 10; Rule 13)
Title: “Suspo-emulsion composition for Plant disease
control”
Applicants: CROP LIFE SCIENCE LIMITED
Address: 209, “PRIMATE”, Near Judges Bunglow Cross Road,
Bodakdev, Ahmedabad-380015, Gujarat, India.
Nationality: An Indian Company incorporated under Indian Companies Act.

FIELD OF THE INVENTION
The present invention relates to a synergistic composition for plant
disease control. More particularly, the present invention relates to a
stable suspo-emulsion composition with enhanced action for plant
disease control, the said composition comprises two active chemical
compounds i.e. (i) Propiconazole of formula (A) and (ii) Tricyclazole of
formula (B). The present invention also relates to a process for the
preparation of the above said suspo-emulsion composition for plant
disease control.
BACKGROUND OF THE INVENTION
Blast disease and sheath Blight Disease occur in Rice plant is
described as follows:
Sheath Blight: Sheath blight was first reported in Japan in 1910.
Since then, it has been reported in almost all rice growing areas of the
world. The disease has become one of the most economically
important diseases of rice throughout the world. Due to its
significance in rice production, extensive research and extension
activities have been underway throughout the world with the aim of
developing integrated management strategies using resistance
varieties, cultural practices, chemical control, and biological control
for effective control of sheath blight.
According to http://www.knowledgebank.irri.org/training/fact-sheets/pest-management/diseases/item/sheath-blight: Sheath blight is a fungal disease caused by Rhizoctonia solani. Infected leaves senesce or dry out and die more rapidly, young tillers can also be destroyed. As a result, the leaf area of the canopy can

significantly be reduced by the disease. This reduction in leaf area, along with the diseased-induced senescence of leaves and young infected tillers are the primary causes of yield reduction.
Sheath blight occurs in areas with high temperature (28-32°C), high levels of nitrogen fertilizer, and relative humidity of crop canopy from 85-100%. Plants are more vulnerable to sheath blight during the rainy season. High seeding rate or close plant spacing, dense canopy, disease in the soil, sclerotia or infection bodies floating on the water, and growing of high yielding improved varieties also favor disease development.
Sheath blight is considered to be an important disease next to rice blast. Rice sheath blight is an increasing concern for rice production especially in intensified production systems. In Japan, the disease has caused a yield loss of as high as 20% and affected about 120,000-190,000 hectares. A yield loss of 25% was reported if the flag leaves are infected. In the United States, a yield loss of 50% was reported when susceptible cultivars were planted. Sheath blight has also caused a yield loss of 6% in tropical Asia.
Blast disease: According to
http://www.knowledgebank.irri.org/index.php?option=com_zoo& task=item&item_id=808&Itemid=739:
Blast is caused by the fungus Magnaporthe oryzae. It can affect all above ground parts of a rice plant: leaf, collar, node, neck, parts of panicle, and sometimes leaf sheath. Blast can occur wherever blast spores are present. It occurs in areas with low soil moisture, frequent and prolonged periods of rain shower, and cool temperature in the daytime. In upland rice, large day-night temperature differences that cause dew formation on leaves and overall cooler temperatures favor the development of the disease.

Rice can have blast in all growth stages. However, leaf blast incidence tends to lessen as plants mature and develop adult plant resistance to the disease.
Rice blast is one of the most destructive diseases of rice. A leaf blast infection can kill seedlings or plants up to the tillering stage. At later growth stages, a severe leaf blast infection reduces leaf area for grain fill, reducing grain yield. Leaf blast can kill rice plants at seedling stage and cause yield losses in cases of severe infection.
Silicon fertilizers (e.g., calcium silicate) can be applied to soils that are silicon deficient to reduce blast. However, because of its high cost, silicon should be applied efficiently. Systemic fungicides like triazoles and strobilurins can be used judiciously to control blast. A fungicide application at heading can be effective in controlling the disease.
Treatment of the above diseases with Fungicides and Pesticides:
Pesticide compounds are often applied in the form of a dilute aqueous composition in order to achieve a good interaction with the target organism, such as plants, fungi and insects. As most active compounds that are used as pesticides, in particular fungicidally active compounds, are only sparingly or even insoluble in water, in most of the cases, formulators are often confronted with difficulties in formulating pesticide compounds in stable formulations.
A number of chemical agents have been used for controlling plant diseases such as Sheath Blight and Blast in Rice plant. However, the problem that plant pathogens have acquired resistance to the chemical agents becomes remarkable due to frequent use or excessive application, etc., of the chemical agents having similar structures and same functions for controlling the same kinds of diseases.
On the other hand, consumers' needs for agricultural chemical-reduced crops and social needs to reduce environmental loads due to agricultural chemicals have now increased. Also, in a farmer's field

where the chemicals have been actually used, when two or more kinds of chemicals are used in admixture for the treatment by the tank mix method, there are many risks to lower the effect of the other chemical to be mixed with each other or possibilities to cause chemical damages against plant materials depending on a combination of chemicals where they are not well-suited to each other.
It has been known that a triazole compounds represented as a fungicide, controlling effects to rice blast (Pyricularia oryzae or Magnaporthe oryzae-a perfect stage of Pyricularia oryzae) and sheath blight (Rhizoctonia solani), by an application method such as foliar spray treatment, etc. Further, several Prior Arts have suggested composition with Propiconazole or Tricyclazole but the biological mode of action of Propiconazole and Tricyclazole permits protective and curative use only and it never gives the satisfactory results with enhanced productivity of the desired plant.
Below are the some of the references that have provided the compositions for controlling plant diseases. US Patent No. 6130236 discloses a fungicidal composition consisting of two systemic fungicides.
US Patent No. 6410574 discloses a mixture of 1,2,3-thiadiazole compound with other systemic fungicidal agents such as isoprothiolane, tricyclazole, azoxystrobin, propiconazole, 8-hydroxyquinoline-copper, oxolinic acid and benomyl.
EP Patent No. 0415569 discloses a fungicidal, herbicidal or plant growth regulating composition for application to an aquatic environment.
Under such a situation, it has been desired to develop a plant disease control composition having high effects against fungi which are resistant to existing chemicals, and having high effects with a low amount of an effective ingredient.

Moreover, for the purpose of preventing plant pathogens from obtaining resistance, it has also been desired to develop a plant disease control composition comprising components (compounds) having different basic structures and different functions with well-suited to each other, and a method for controlling plant diseases.
OBJECTS OF THE INVENTION
Accordingly, the main object of the present invention is to provide a
synergistic suspo-emulsion composition for Sheath Blight and Blast disease treatment in Rice Plant.
It is an object of the present invention to provide improved, stable and ready to use suspo-emulsion composition having synergistic and superior properties in treating Sheath Blight and Blast disease in Rice Plant compared to prior art formulations or mixtures or compositions.
It is further an object of the present invention to provide a suspo-emulsion composition that will protect crop from fungal diseases like sheath blight and blast.
Yet it is an object of the present invention to provide a suspo-emulsion composition for Sheath Blight and Blast disease treatment in Rice Plant wherein the said composition comprises two active chemical compounds (i) Propiconazole of formula (A) and (ii) Tricyclazole of formula (B).
Another object of the present invention is to provide a process for the preparation of suspo-emulsion composition for Sheath Blight and Blast disease treatment in Rice Plant.
It is also an object to provide a method of application of composition to the plants to control Sheath Blight and Blast disease in Rice Plant.
BRIEF DESCRIPTION OF DRAWING
Figure-1 is a flow diagram of the process for the preparation of synergistic plant disease control composition.

Figure-2 is a photographic image of the symptoms of Sheath Blight disease in rice crop.
Figure-3 is a photographic image of the symptoms of Blast disease in rice crop.
DETAIED DESCRIPTION OF THE INVENTION
The present invention contains two active chemical compounds that
mutually complement each other when used together and exhibit synergistic plant disease control capabilities that is greater than the individual compound’s capability to control the plant disease when used alone.
The present innovators have comprehensively and successfully investigated the possibility of developing a synergistic suspo-emulsion composition comprising Propiconazole of formula (A) and Tricyclazole of formula (B).
An aqueous suspo-emulsion composition is a mixture of water-insoluble active ingredients dispersed in an aqueous solution, where one (or more) of the active ingredients is in suspension form and one (or more) of the active ingredients is in emulsion form. The formulation is intended for dilution into water prior to spray application. Mixtures of active ingredients are often used to provide a broader spectrum of pest control. Formulating the active ingredients together eliminates the need for tank mixing (which can lead to incompatibilities). Like other aqueous liquid formulations, suspo-emulsions are easy to handle and measure, dust free, non-flammable and offer good miscibility with water.
According to the first embodiment of the present invention, there is provided a suspo-emulsion composition for plant disease control wherein a composition may comprise two active chemical compounds i.e. Propiconazole of formula (A) and Tricyclazole of formula (B). Propiconazole of formula (A) is soluted in a selected organic solvent

and a tricyclazole of formula (B) is simultaneously present in form of solid particle in aqueous media.
Accordingly, the present invention relates to a stable synergistic suspo-emulsion composition, a suspo-emulsion composition comprises: (i) an effective amount of propiconazole of formula (A) in a range between 10.1% to 11.2% (W/W) dissolved in an organic solvent; (ii) an effective amount of tricyclazole of formula (B) in a range between 32.4% to 36% (W/W) in aqueous medium with a particle size of 5 to 50 microns; (iii) auxiliaries used in stage-(i) and stage-(ii) to prepare suspo-emulsion composition; and optionally (iv) viscosity agent or deformer for adjusting viscosity of suspo-emulsion composition.
It has been found surprisingly that suspo-emulsion compositions of the present invention contain water as continuous phase, Propiconazole in organic solvent and a tricyclazole in the form of dispersed particles wherein the particles of tricyclazole are homogeneously distributed in the suspo-emulsion composition and that enable an improved plant disease control activity of the said compositions.
Propiconazole is a sterol demethylation ergosterol biosynthesis) inhibitor. It is a systemic foliar fungicide with protective and curative action, with translocation acropetally in the xylem.
Tricyclazole is a melanin biosynthesis inhibitor (reduction of 1, 3, 8-trihydroxynaphthalene) and works as systemic fungicide, absorbed rapidly by the roots, with translocation through the plant and used in the control of rice blast in transplanted and direct-seeded rice.

It has also been found that the physical states of the active ingredients are maintained after long storage, thus affording sprayable liquid containing the solubilised compound Propiconazole of formula (A) in the organic solvent and fine particles of the Tricyclazole of formula (B).
The active chemical compounds Propiconazole of formula (A) and Tricyclazole of formula (B) are commercially available with technical grade specification. If desirous, one can also prepare the above compounds with the help of the processes reported in the prior art references.
The composition of the present invention contains auxiliaries to be used in stage-(i) and stage-(ii) in the process for preparing suspo-emulsion composition. In order to prepare stable, ready to use suspo-emulsion compositions, Auxiliaries may be selected from but not limited to emulsifiers or dispersing agent, wetting agents, antifreeze agents, antifoaming agents, thickeners, preservatives and binders.
For the purpose of suspo-emulsion composition, organic solvent and auxiliaries may be selected from the list provided in the following paragraphs:
At least one organic solvent is selected from water, methyl soyate (MSO), ethyl acetate, ethylene glycol, glycerine, heptane, hexamethylphosphoramide (HMPA), Hexamethylphosphorous triamide (HMPT), methanol, methyl t-butyl ether (MTBE), Toluene, Mix xylene, Acetone, Acetonitrile.
At least one emulsifier or dispersing agent is selected from a group comprising polyvinylpyrrolidone, polyvinylalcohol, lignosulphonates, phenyl naphthalene sulphonates, phenyl naphthalene sulphonates, ethoxykated alkyl phenols, ethoxylated fatty acids, alkoxylated linear alcohols, polyaromatic sulphonates, glyceryl esters, maleic anhydride copolymers, phosphate esters, condensation products of aryl

sulphonic acids and formaldehyde, condensation product of aryl sulphonic acids and formaldehyde, addition product of ethylene oxide and fatty acid esters, lignin derivatives, naphthalene formaldehyde condensates, sodium salts of iso decylsulfosuccinic acid half ester, polycarboxylates, sodium alkylbenesulfonates, sodium salts of sulfonated naphthalene, ammonium salts of sulfonated naphthalene, salts of polyacrylic acids, salts of phenolsulfonic acids and salts of naphthalene sulfonic acids.
Wherein, emulsifier or dispersing agent may be added in a range of 1% to 25% (W/W).
At least one wetting agent is selected from a group comprising phenyl naphthalene sulphonates, alkyl naphthalene sulfonates, sodium alkyl naphthalene sulfonate, sodium salt of sulfonated alkylcarboxylate, polyoxyalkylated ethyl phenols, polyoxyethoylated fatty alcohols, polyoxyethoxylated fatty amines, lignin derivatives, alkene sulfonates, alkykbenzene sulfonates, salts of polycarbozylic acids, salts of esters of sulfosusuccinic acid, alkylnaphthalenesulphonates alkylbenzenesulfonates, alkylpolyglycol ether sulfonates, alkyl ether phosphates, alkyl ether sulphates and alkyl sulfosuccinic monoesters. Wherein, wetting agent may be added in a range of 0.1% to 5% (W/W).
At least one antifoaming agent selected from a group comprising polyvinyl alcohols, phenyl naphthalene sulphonate, lignin derivatives, polyvinyl pyrrolidone, polyalkylpyrrolidone, carboxymethylcellulose, xanthan gum, polyethoxylated, fatty acids, polyethoxylated fatty alcohols, ethylene oxide copolymer, propylene oxide copolymer, polyethylene glycols and polyethylene oxides.
Viscosity agent or deformer may be added for adjusting viscosity of the suspo-emulsion composition for example but limited to xanthan gum. The other examples of viscosity agent or deformer is a group comprising polyvinyl alcohols, phenyl naphthalene sulphonate, lignin derivatives, polyvinyl pyrrolidone, polyalkylpyrrolidone,

carboxymethylcellulose, xanthan gum, polyethoxylated, fatty acids, polyethoxylated fatty alcohols, ethylene oxide copolymer, propylene oxide copolymer, polyethylene glycols and polyethylene oxides.
Wherein, antifoaming agent or viscosity agent or deformer may be added in a range of 0.1% to 10% (W/W).
More particularly, the present invention relates to a stable synergistic suspo-emulsion composition containing propiconazole and tricyclazole in a total amount of 42-48% (W/W) and having 5-15 microns particle size of suspended particles in composition, a suspo-emulsion composition for the treatment of Sheath Blight and Blast disease in Rice plant comprises:
Propiconazole in an amount between 10.1-11.2% (W/W); Tricyclazole with a particle size 10 to 90 microns in amount between 32.4-36% (W/W);
water in an amount of 35-40% (W/W) and methyl soyate (MSO) in an amount of 10-12%(W/W) as solvents; emulsifiers propylene glycol, agnique 72L, Tamol FBP1, SC 1316, each emulsifier in an amount of 0.3-2% (W/W); silicon deformer in an amount of 0.4-0.8% (W/W); formaldehyde in an amount of 0.4-0.6% (W/W) as preservative to give stability to the formulation; xanthan gum as viscosity agent in an amount of 0.19-0.22%(W/W).
Said suspo-emulsion composition is having suspensibility between 90-95% and viscosity between 50-75 seconds.
According to second embodiment of the present invention, there is also a process provided for the preparation of suspo-emulsion composition for controlling plant disease.
Figure-1 is a flow diagram of the process for the preparation of a suspo-emulsion composition for plant disease control. Accordingly, a process for the preparation of a synergistic suspo-emulsion composition containing propiconazole and tricyclazole in a total

amount of 42-48% (W/W) and having 5-15 microns particle size of suspended particles in composition, said process for preparing synergistic suspo-emulsion composition for the treatment of Sheath Blight and Blast disease in Rice plant comprises:
i. Mixing solvent, emulsifiers, slowly adding 32-36% (W/W) tricyclazole having particle size between 10 to 90 microns & silicon deformer, wet grinding it to get reaction solution-A;
ii. mixing solvent, 10-12% (W/W) propiconazole, emulsifier and stir it till get clear solution-B;
iii. mixing reaction solution-A & solution-B under high stirring;
iv. adding viscosity agent and as preservative to give suspo-emulsion composition.
Said suspo-emulsion composition is having suspensibility between 90-95% and viscosity between 50-75 seconds.
The whole process is carried out at a temperature between 50C to 400C.
In the above suspo-emulsion composition, tricyclazole compound present in the form of suspended particles in a suspo-emulsion composition.
Particle size of suspended particles of tricyclazole in a ready suspo-emulsion solution may vary between 0.5 to 75 microns, more preferably 5-15 microns.
The other factors and parameters of the process may be provided as
follows:
At least one organic solvent is selected from water, ethyl acetate,
ethylene glycol, glycerine, heptane, hexamethylphosphoramide
(HMPA), Hexamethylphosphorous triamide (HMPT), methanol, methyl
t-butyl ether (MTBE), Toluene, Mix xylene, Acetone, Acetonitrile, more
preferably water or glycerine is used as organic solvent.

At least one emulsifier or dispersing agent is selected from a group comprising polyvinylpyrrolidone, polyvinylalcohol, lignosulphonates, phenyl naphthalene sulphonates, phenyl naphthalene sulphonates, ethoxykated alkyl phenols, ethoxylated fatty acids, alkoxylated linear alcohols, polyaromatic sulphonates, glyceryl esters, maleic anhydride copolymers, phosphate esters, condensation products of aryl sulphonic acids and formaldehyde, condensation product of aryl sulphonic acids and formaldehyde, addition product of ethylene oxide and fatty acid esters, lignin derivatives, naphthalene formaldehyde condensates, sodium salts of iso decylsulfosuccinic acid half ester, polycarboxylates, sodium alkylbenesulfonates, sodium salts of sulfonated naphthalene, ammonium salts of sulfonated naphthalene, salts of polyacrylic acids, salts of phenolsulfonic acids and salts of naphthalene sulfonic acids.
Wherein, emulsifier or dispersing agent may be added in a range of 1% to 25% (W/W).
At least one wetting agent is selected from a group comprising phenyl naphthalene sulphonates, alkyl naphthalene sulfonates, sodium alkyl naphthalene sulfonate, sodium salt of sulfonated alkylcarboxylate, polyoxyalkylated ethyl phenols, polyoxyethoylated fatty alcohols, polyoxyethoxylated fatty amines, lignin derivatives, alkene sulfonates, alkykbenzene sulfonates, salts of polycarbozylic acids, salts of esters of sulfosusuccinic acid, alkylnaphthalenesulphonates alkylbenzenesulfonates, alkylpolyglycol ether sulfonates, alkyl ether phosphates, alkyl ether sulphates and alkyl sulfosuccinic monoesters. Wherein, wetting agent may be added in a range of 0.1% to 5% (W/W).
At least one antifoaming agent selected from a group comprising polyvinyl alcohols, phenyl naphthalene sulphonate, lignin derivatives, polyvinyl pyrrolidone, polyalkylpyrrolidone, carboxymethylcellulose, xanthan gum, polyethoxylated, fatty acids, polyethoxylated fatty alcohols, ethylene oxide copolymer, propylene oxide copolymer, polyethylene glycols and polyethylene oxides.

Viscosity agent or deformer may be added for adjusting viscosity of the suspo-emulsion composition for example but limited to xanthan gum. The other examples of viscosity agent or deformer is a group comprising polyvinyl alcohols, phenyl naphthalene sulphonate, lignin derivatives, polyvinyl pyrrolidone, polyalkylpyrrolidone, carboxymethylcellulose, xanthan gum, polyethoxylated, fatty acids, polyethoxylated fatty alcohols, ethylene oxide copolymer, propylene oxide copolymer, polyethylene glycols and polyethylene oxides.
Wherein, antifoaming agent or viscosity agent or deformer may be added in a range of 0.1% to 10% (W/W).
Based on the description of the general process for the preparation of Suspo-emulsion composition, it is our intension to provide a specific process for the preparation of suspo-emulsion composition.
Accordingly, In a preferred embodiment, a process for the preparation of a stable synergistic suspo-emulsion composition containing propiconazole and tricyclazole in a total amount of 42-48% (W/W) and having 5-15 microns particle size of suspended particles in composition, at a temperature between 5-400C, said process for preparing a stable synergistic suspo-emulsion composition for the treatment of Sheath Blight and Blast disease in Rice plant comprises:
i. Mixing water, propylene glycol, SC1316 & Tamol (FBP1), slowly adding 32-36 % (W/W) tricyclazole having particle size between 10 to 90 microns & silicon deformer, wet grinding it to get reaction solution-A;
ii. mixing methyl soyate (MSO) as solvent, 10-12% (W/W) propiconazole, Agnique 72L as emulsifier and stir it till get clear solution-B;
iii. mixing reaction solution-A & solution-B under high speed stirring;

iv. adding xanthan gum as viscosity agent and formaldehyde as preservative to give suspo-emulsion composition.
Said suspo-emulsion composition is having suspensibility between 90-95% and viscosity between 50-75 seconds.
In step-(i), after mixing of solvent & emulsifiers in SS reactor, tricyclazole was slowly added into the mixture at a temperature between 5-400C to avoid lump formation. The addition is so tricky and complex as it will forms lumps in the mixture because in case if lumps formed during the addition, it will not give desired particle size of Tricyclazole suspended particles into the final composition and thus main objective of achieving suspo-emulsion composition with tricyclazole suspended particles with a particle size between 5-15 microns not resulted. Temperature range between 5-400C and selection of solvent, emulsifiers and silicone deformer also play an important role to maintain desired particle size of tricyclazole suspended particles in the suspo-emulsion composition. Once addition of tricyclazole is over, mixture is taken for wet grinding using prior art methods, more particularly grinding was done with zirconia beads to achieve desired particle size between 5-15 microns of tricyclazole suspended particles. Thus formed is called as reaction mixture-A.
Reaction mixture-B is prepared as per the process provided in the step-ii.
In the third step-iii, reaction mixture-A and reaction mixture-B is mixed at high stirring. More particularly, mixing was done by adding reaction mixture-B nto the reaction mixture-A at a temperature between 5-400C under high stirring. Speed of high speed stirring was maintained between 15-500 RPM. Temperature range between 5-400C and speed of stirring play an important role during mixing. If addition of reaction mixture-B into reaction mixture-B is not done under high speed stirring, lump formation may found and thus it will destroy the objective of achieving suspo-emulsion composition with tricyclazole

suspended particles in desired particle size between 5-15 microns. Once addition of reaction mixture-B is over, viscosity agent to adust viscosity and formaldehyde as preservative was added into the mixture and stirred the reaction mass for half an hour to give suspo-emulsion composition with desired results.
No prior art has suggested the suspo-emulsion composition with a particle size 5-15 microns of suspended particles in the composition. Further, higher suspensibility with viscosity between 50-75 seconds will increase the dispersion of the particles in the composition and thus nullify the deposition of particles in the bottom of the spray tank while spraying in the field or farm using spray tank. It will also increase the stability of the composition.
Suspo-emulsion composition prepared by the said process is stable for 2 years and no adverse effect has been observed in the composition during the storage of suspo-emulsion composition for 2 years even at 540C temperature. Thus due to its stability for a longer period of time, synergistic characteristics like higher suspensibility with a viscosity of 50-75 seconds and increased dispersion of the particles in the composition, composition shall be considered as the best suspo-emulsion in its class.
Suspo-emulsion composition of the present invention is compared with the Propiconazole (25%) EC, Tricyclazole (75%) WP, Hexaconazole (5%) EC, the results have been provided with this application that show that suspo-emulsion composition of the present invention is better than the other composition in terms of stability, suspansibility, viscosity and gives synergistic effects in testing of bio-efficacy in controlling Sheath Blight and Blast disease on Rice Plant and phyto-toxicity on Rice Plant.

EXAMPLE(S) OF THE PRESENT INVENTION
Now without limiting the scope of the present invention, a process for
the preparation of suspo-emulsion composition at a temperature
between 50C-400C, may be described as follows:
(i) in a stainless steel reactor water, SC 1316, Tamol FBP1, Propylene
glycol have been charged and stirred for half an hour. Tricyclazole
having particle size between 10 to 90 microns is added into the reactor
under stirring, Silicon deformer is added into the above mixture and
mixture is taken for wet grinding to get reaction mixure-A.
(ii) In another reactor, Methyl soyate, propiconazole, agnique 72L are
mixed under stirring to get reaction mixure-B.
(iii) Reaction mixture-B is added into reaction mixture-A under high
speed stirring.
(iv) Xanthan gum as viscosity agent and formaldehyde as preservative
are added into the above mixture and stirred for 30 minutes to give
desired suspo-emulsion composition.
Result:
Propiconazole (% W/W) by HPLC: 10.7%W/W
Tricyclazole (%W/W) by HPLC: 34.2%W/W
Description: Light orange to ten colored viscous liquid
Suspensibility: 93.10%
Viscosity: 70 seconds
Spacific gravity: 1.14 gm/ml
Particle size (Using Mastersizer 2000): 5-10 micron
D-10: 10.60%
D-50: 0.03%
D-90: 0.00%
Suspo-emulsion composition containing 10.78% Propiconazole &
34.27% Tricyclazole (equivalent to 125 g and 400 g of Propiconazole
and Tricyclazole, Total 44.9% SE (Also Known as CLSL Rust Blast
44.9% SE), respectively in a litre of a suspo-emulsion composition) is
recommended for the control of sheath blight and blast disease in
paddy.

BIO-EFFICACY, PHYTO-TOXICITY AND STABILITY STUDY OF COMPOSITION:
Figure-2 shows symptoms of Sheath Blight Disease in rice crop while Figure-3 shows symptoms of Blast disease in rice crop. The suspo-composition of the present invention was sent to Banaras Hindu University (BHU) for further analysis of Bio-efficacy for controlling Sheath Blight and Blast diseases and Phyto-toxicity on Paddy (Rice) crop. Rust Blast 44.9% SE (Trading name of suspo-emulsion composition of the present invention) @ 600 ml/ha was found most effective against Sheath Blight and Blast diseases in rice crop. It controls both diseases in preventive and curative mode of action. It did not cause any phyto-toxic symptoms on rice plants @ 500ml/ha to 1200 ml/ha after spraying. The plants were healthy subsequent to the spraying.
BIOEFFICACY OF SUSPO-EMULSTION COMPOSITION
Letter dated December 14, 2017 along with results shows that the composition of the present invention has excellent effect in the treatment of Sheath Blight and Blast diseases on Paddy (Rice) crop.
Figure-2 shows the symptoms of Sheath Blight disease in rice crop while figure-3 shows the symptoms of Blast disease in rice crop.
Table-1: Treatment details for Bio-efficacy of different fungicides on Rice Plant:

Sr. No. Fungicide selected for treatment Dosage of Fungicide (ml or Gm/Ha)
1 Rust Blast 44.9% SE 500
2 Rust Blast 44.9% SE 600
3 Rust Blast 44.9% SE 700
4 Propiconazole 25% EC 500
5 Tricyclazole 75% WP 400
6 Hexaconazole 5% EC 1000
7 Untreated control (Water only) --
Method of Treatment:
Experimental trial was conducted with all standard and recommended
packages of agronomic practices such as tillage, transplanting,

spacing, manuring, weed management, irrigation and insect contro for cultivation of rice crop. In case of Blast disease of rice, first symptom was observed at 20 days after transplanting (DAT) whereas Sheath Blight of rice, first symptom was observed at 33 DAT. The test fungicides were applied as foliar spray with sprayer. First spraying was done at 20 days after transplanting (DAT). These were followed by two other sprays at 15 days interval. The percentage disease severity was calculated by the following formula. The severity of the disease was recorded on day before each spraying.
PDI = Sum of all numerical ratings X 100

Total Plants observed X Maximum ratings scale
The present disease severity values were transformed by angular transformation and analyzed statistically.
Method of disease scoring:
For Sheath Blight of rice, 0-9 scale (As per IRRI, 1996) was used
for disease scoring:

Sr. No. Description Score
1 No infection 0
2 Vertical spread of the disease up to 20% of plant height 1
3 Vertical spread of the disease up to 21-30% of plant height 3
4 Vertical spread of the disease up to 31-45% of plant height 5
5 Vertical spread of the disease up to 46-65% of plant height 7
6 Vertical spread of the disease more than 66% of plant height 9
For Blast disease in Rice Plant, the 0-9 scale (IRRI, 1996) was used for disease scoring:
Sr. No. Description Score
No lesions 0
1 Small brown specks of pinhead size without sprouting center 1

2
Small roundish to slightly elongated grey spot about 1-2 mm inn diameter with a distinct brown margin and lesions are on lower surface 2
3 Same as score 2 but significant number of lesions on upper surface 3
4 Typical sporuting blast lesions, 3 mm or longer, infecting < 2% of leaf area 4
5 Typical blast lesion infecting 2-10% of the leaf area 5
6 Typical blast lesion infecting 11-25% of the leaf area 6
7 Typical blast lesion infecting 26-50% of the leaf area 7
8 Typical blast lesion infecting 51-75% of the leaf area 8
9 Typical blast lesion, >75% infected leaf area 9
Statistical Analysis: Analysis was done using Analysis of variance (ANOVA) using SPSS-19 Computer Software Package.
Results:
A) Efficacy of CLSL-Rust Blast 44.9% SE against Sheath Blight
disease severity of Rice:
Three sprays of the test chemicals and the fungicides were given at the interval of 15 days, first being at initial appearance of the disease symptoms. Data on Sheath Blight disease severity were recorded and presented in Table-2. All the treatments reduced the disease severity of Sheath Blight of rice significantly compared to untreated check plot. CLSLS Rust Blast 44.9% SE @ 600 ml/ha and 700 ml/ha were proved to be the best fungicide treatments as compared to other fungicides shown in table. CLSL Rust blast 44.9% SE 500ml/ha reducing disease incidence by 87.56% and 90.67% compared to control plants after three sprays. Similar results is also evident for disease reduction after second spray. Since disease reduction by CLSL Rust Blast 44.9% SE @ 700ml/ha and 600 ml/ha are not significantly different from each other, the lower dose i.e. CLSL Rust Blast 44.9% SE @ 600 ml /ha has pproven to be the best fungicide reducing the disease incidence by 87.56% after third spray (Table-2).


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B) Efficacy of CLSL-Rust Blast 44.9% SE against Blast Disease severity of Rice Plant:
Above Table-3 shows that CLSL-Rust Blast 44.9% SE @ 600 ml/ha and 700 ml/ha were proved to be the best fungicide treatments as

compared to other fungicides shown in Table-3. CLSL- Rust Blast 44.9% SE @ 500 ml/ha reducing disease incidence by 67.00% and 73.09% compared to control plants after three sprays. Similar result is also evident for disease reduction after second spray. Similar to the result for Sheath Blight disease of Rice, disease reduction by CLSL Rust Blast 44.9% SE @ 600 ml/ha and 700 ml/Ha are not significantly different from each other. Therefore lower dose i.e.e CLSL Rust blast 44.9% SE @ 600 ml/ha may be considered as the best treatment for the management of Blast of Rice Plant. Thus CLSL- Rust Blast 44.9% SE @ 600 ml/ha has proven to be te best fungicide reducing the disease incidence by 67.99% after third spray (Table-3). C) Yield attribution of Fungicide: Significant increase in yield was obtained from all the fungicidal treated plots s compared to untreated check. The highest yield of Rice was obtained from CLSL Rust Blast 44.9% SE @ 700 ml/ha followed by CLSL Rust blast 600 ml/ha as shown in Table-4. CLSL Rust Blast 44.9% SE 700 ml/ha increase yield of Rice by 22.2.%.
Table-4:


Conclusion: Based on the results, it may be concluded that the spraying of the fungicide CLSL-Rustblast 44.9% SE @ 600 ml/ha and 700 ml/ha thrice, starting from the appearance of the disease at 15 days intervals were found to be excellent against Sheath Blight and Blast diseases of Rice Plant and also for the enhancement of the yield of Rice over other fungicides.
Further, CLSL Rust Blast 44.9% SE gave the best control in comparison of other fungicides against both diseases of Rice crop. Rust Blast 44.9% SE controls Sheath Blight and Blast diseases as preventive and curative mode of action and gave excellent results.
PHYTO-TOXICITY EFFECT OF FURNGICIDES ON RICE PLANT:
Following Fungicides have been taken for Phyto-toxicity testing: RustBlast 44.9% SE @ 500 ml/ha RustBlast 44.9% SE @ 600 ml/ha RustBlast 44.9% SE @ 700 ml/ha
Place of Trial: Agricultural Research Farm BHU, Varanasi, Uttarpradesh.
A trial was conducted at metioned place in order to evaluate Phyto-toxicity of different dosage of fugnicides on Rice Plant at the stage of vegetative growth of the plant. The experiment was laid out in field randomized block design with six treatments and three replications.
The trial was conducted as per prescribed for the purpose by the CIB-Registration Committee. Observations on Study are as follows:

Table J: Symptoms of leaf injury before and after Spraying of CLSL-RttStBlasI 44.9% SF. on rice,

1 rear merits Dates of observation

12.0! (Befc ,:oi7 i ;.ii-.'.:(ii7 re spray) (1 DAS) 15.08.2017 19.08.21 (3 DAS) (7 DAS )17 ) 22,08,2017 (10 DAS) 27.08.2017 (15 DAS)

Rl R2 R3 Rl R2 :R3 Rl R2 R.I Rl R2 R3 Rl R2 R3 Rl !R2 !R3
Tl: Untreated control (water spraj) M 0 0 1) 0 II 0 0 0 0 0 0 0 "> 0 11 0 «
T2: CLSL -RustBlast rg 500 ml/ha 0 0 0 II (1 (1 0 0 0 0 0 0 0 0 0 (1 0 u
T3: CLSL-RustBlEsttf 600 ml/ha 0 0 0 (I 0 0 0 I) 0 0 l.l 0 0 0 () 0 0 0
T4: CLSL-RustBlast a 700 ml/ha « <) 0 () 0 0 0 i) o a 'I 0 0 » u 0 0 0
T5:CLSL-RuslB]as«a 1200 ml/ha 0 0 o 0 0 0 0 H 0 .0 0 0 0 » u 0 .0 0
N. B.: 0: No phytolcxicily; DAS: Days Alter Spray
All the above treatments did not caused leaf injury to rice plant
Table 2: Symptoms of wilting befitre and after spraying: CLSL-KustBlast on rice.

Dales of obser\ations
Treatments 12.08.2017 (Before spray) 13.07.2 {IDAS )17 ) 15.08.2017 (3 DAS) 19.08.2017 {7 DAS) 22.08.2017 (10 DAS) 27,08.2017 {15 DAS)

Rl R2 R3 Rl R: R3 Rl R: R3 Rl R2 R3 Rl R2;R3 Kl R2 R3
Tl: Untreated control (water spray) 0 0 0 0 0 0 0 0 0 0 0 0 £_ 0 0 0 0 0
T2: CLSL -RustBlast £ 500 ml/ha 0 0 0 10 0 0 0 0 0 0 0 0 0 D 0 0 0 0
T3: CLSL -RustBlast® 600 ml/ha 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 C 0
T4: CLSL -RustBlast ft; 700 ml/ha ;0 0 0 0 0 0 0 0 0 0 ° 0 0 0 0 p 0 0
T5: CLSL -RustBlast'g 1200 ml/ha |o 0 O'O 0 0 0 0 0 0 0 0 0 0 0 0 0 0

1 real merits Dates of observations

12.08.2017 (Before sprav) 13.07.2017 (1 DAS) Li .08.2 (3 DAS )17 19.08.2017
1 1(7 DAS) 22.08.2017 (10 DAS) 27.08.2017 (15 DAS)

Rl IC RJ Rl R2 R.1 Rl R2 R3 Rl R2 R3 Rl R2 R3 Rl IR2 R3
Tl: Untreated control (water spray) 0 0 0 0 0 0 C 0 0 0 0 0 0 0 0 0 0 0
T2: CLSL -RustBlast @ 500 ml/ha 0 0 0 0 0 0 c 0 0 0 0 0 0 0 0 0 0 0
T3: CLSL -RustBlast® 600 mi/ha 0 0 0 0 0 0 c 0 0 0 0 0 0 0 0 0 0 0
T4: CLSL -RustBlast ®. 700 ml/ha 0 o 0 0 0 0 c 0 0 0 0 0 0 0 0 0 0 0
T5:CLSL-RuslBlast® 1200 ml/ha 0 o 0 0 jo JO |0 0 0 0 0 0 0 0 0 0 0 lO
N. R,: 0: No phytntnxictty: DAS' Days Afcr SpraAll the ahove treatments did not caused vein clearing in rice plant.
Observations:
From the results presented in Table-1-6, it could be concluded that CLSL-Rust Blast 44.9% SE was not found to cause any phytotoxic symptoms on Rice Plant. The Plants were healthy subsequent to the

spraying. Thus CLSL-Rust Blast 44.9% SE is safe to use on Rice Plant at the dosage from 500 ml/ha to 1200 ml/ha.
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Tabit V Ss«tpliia» Wifw«rf» IK ruff «nH 4lhfr ^rnim t« LSI.-KlMtWwl ot rice.

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STABILITY STUDY OF SUSPO-EMULSION COMPOSITION:
Stability of the disclosed Suspo-emulsion composition was observed using procedure shown in CIPAC method 1999; prepared by the German formulation panel (DAPF). Accordingly, said suspo-emulsion composition was kept in a bottle and stored for 14 days at a temperature 540C, sample was analyzed for adverse effect, if any. It has been observed that there is no adverse effect or increase of any impurity in the said suspo-emulsion and is stable even at 540C temperature.
ADVANTAGES OF THE INVENTION
A suspo-emulsion composition of the present invention shows a broad spectrum against various plant pathogens (Rice Blast (Pyricularia oryzae or Magnaporthe oryzae-a perfect stage of Pyricularia oryzae) and Sheath Blight (Rhizoctonia solani) etc.) including fungi resistant to chemicals, and shows excellent controlling effects (synergistic controlling effects) which could never be expected from a single component alone.
Also, it shows high plant disease controlling effects against existing fungi resistant to chemicals, and no chemical damage against plants can be admitted.

We claim:
1. A stable synergistic suspo-emulsion composition containing
propiconazole and tricyclazole in a total amount of 42-48%
(W/W) and having 5-15 microns particle size of suspended
particles in composition, a suspo-emulsion composition for the
treatment of Sheath Blight and Blast disease in Rice plant
comprises:
Propiconazole in an amount between 10.1-11.2 % (W/W); Tricyclazole with a particle size 10 to 90 microns in amount between 32.4-36 % (W/W);
water in an amount of 35-40% (W/W) and methyl soyate (MSO) in an amount of 10-12%(W/W) as solvents; emulsifiers propylene glycol, agnique 72L, Tamol FBP1, SC 1316, each emulsifier in an amount of 0.3-2% (W/W); silicon deformer in an amount of 0.4-0.8% (W/W); formaldehyde in an amount of 0.4-0.6% (W/W) as preservative to give stability to the formulation; xanthan gum as viscosity agent in an amount of 0.19-0.22%(W/W),
Said suspo-emulsion composition is having suspensibility between 90-95% and viscosity between 50-75 seconds.
2. The synergistic suspo-emulsion composition as claimed in claim 1 wherein tricyclazole is present as suspended particles in composition.
3. A process for the preparation of a stable synergistic suspo-emulsion composition as claimed in claim 1 wherein propiconazole and tricyclazole in a total amount of 42-48% (W/W) and having 5-15 microns particle size of tricyclazole suspended particles in composition, at a temperature between 5-400C, said process for preparing a stable synergistic suspo-emulsion composition for the treatment of Sheath Blight and Blast disease in Rice plant comprises:

i. Mixing water, propylene glycol, SC1316 & Tamol (FBP1),
slowly adding 32-36 % (W/W), tricyclazole having particle
size between 10 to 90 microns & silicon deformer, wet
grinding it to get reaction solution-A; ii. mixing methyl soyate (MSO) as solvent, 10-12% (W/W)
propiconazole, Agnique 72L as emulsifier and stir it till get
clear solution-B; iii. mixing reaction solution-A & solution-B under high speed
stirring; iv. adding xanthan gum as viscosity agent and formaldehyde as
preservative to give suspo-emulsion composition,
Said suspo-emulsion composition is having suspensibility between 90-95% and viscosity between 50-75 seconds.
4. A stable synergistic suspo-emulsion composition containing propiconazole and tricyclazole in a total amount of 44.9% (W/W) and having tricyclazole as suspended particles with a particle size of 5-15 microns in composition, a suspo-emulsion composition for the treatment of Sheath Blight and Blast disease in Rice plant comprises: Propiconazole in an amount between 10.7% (W/W); Tricyclazole with a particle size 10 to 90 microns in amount between 34.2% (W/W);
water in an amount of 38.4% (W/W) and methyl soyate (MSO) in an amount of 9.97%(W/W) as solvents; emulsifiers propylene glycol in an amount 0.69%(W/W), agnique 72L in an amount 0.37%(W/W), Tamol FBP1 in an amount 0.49%(W/W), SC 1316 in an amount 1.49%(W/W); silicon deformer in an amount of 0.49% (W/W); formaldehyde in an amount of 0.49% (W/W) as preservative to give stability to the formulation; xanthan gum as viscosity agent in an amount of 0.199%(W/W),

Said suspo-emulsion composition is having suspensibility between 90-95% and viscosity between 70 seconds.