FORM 2
THE PATENT ACT, 1970
(39 of 1970)
&
The Patents Rules, 2003
COMPLETE SPECIFICATION
(See section 10 and rulel3)
LITTLE OF THE INVENTION:
"SUSTAINABLE COLD-DISPERSIBLE PEARLESCENT CONCENTRATE"
2. APPLICANT
(a) NAME: GALAXY SURFACTANTS LTD.
(b) NATIONALITY: An Indian Company incorporated under the Indian
Companies ACT, 1956
(c) ADDRESS: C-49/2, TTC Industrial Area, Pawne,
Navi Mumbai- 400703 Maharashtra, India
3. PREAMBLE TO THE DESCRIPTION
The following specification particularly describes the invention and the manner in which it is to be performed.

FIELD OF INVENTION
The present invention relates to free-flowing, cold-dispersible, pearlescent concentrates for personal care and home care compositions. Particularly, these pearlescent concentrates are made without alkyl sulphates / alkyl ether sulphates, alkanol amides, alkylamidopropyl betaines and esters of ethylene glycol which are harmful ingredients. More particularly, the present invention relates to pearlescent concentrates comprising of mild surfactants such as glycinates and isethionates and pearlizing agent such as propane diol diesters. Further, the pearlescent concentrate is preserved without employing parabens, isothiazolinones or formaldehyde releasers.
BACKGROUND OF INVENTION
in which R1 denotes C13 to C21 alkyl group, R2 is H or R1CO and R3 is C8 to C22 alkyl group.
Personal care compositions such as face-washes, body-washes, hand-washes and shampoos etc. are made to look aesthetically attractive by incorporation of the pearlescent additives. These pearlescent additives impart iridescent sheen or glow to personal and home care formulations. The examples of commonly used pearlescent additives are monoesters or diesters of ethylene glycol (Formula I), propylene glycol, oligomeric alkylene glycols, glycerol esters of fatty acids, typically higher carbon chains with C14 to C22 and monoalkanol amides of fatty acids (Formula II).


A typical procedure for converting the duil appearance of cosmetic formulations (face-wash, body-wash, shampoo and other aqueous compositions of surfactants) into a pearly (shiny) look involves addition of pearlescent additive (pearly wax) like distearate of ethylene glycol to the entire formulation and heating under agitation above the melting points of the pearlescent additives (70-80°C) to ensure homogeneous mixture. This is then followed by cooling the entire mass gradually under slow agitation. This way, the pearly waxes are allowed to crystallize into platelet like structures so that the uniformly dispersed platelets reflect light imparting the shine to the products.
Developing right size and shape of pearly wax is a kind of complex technology (Norbert Boyxen in Olaz, Szappan, Kosmetica, 50 evofolyam (2001) and M. A. Bolzinger et al., Colloids and Surfaces A: Physiochem. Eng. Aspects, 93-100, 299 (2007) and getting the consistency in pearly sheen of the finished personal care products, batches after batches, is not always easy. This problem has been addressed by the 'easy-to-use' cold-dispersible pearlescent concentrates.
The surfactants manufacturing industry developed the technology to manufacture so called 'cold-dispersible', 'free-flowing' pearlescent concentrates for the ease of incorporation of pearly waxes into personal and home care formulations (US Pat 4,777,038, 4,824,594, 4,948,528 & 5,017,305). These 'cold-dispersible pearlescent concentrates' are nothing but pearl waxes dispersed in the crystal form in aqueous surfactant systems. These are very stable and concentrated, yet flowable, dispersions, that are required to be added anywhere between 0.5 to 5.0 % to the personal or home care compositions to impart the pearlescent appearance. This operation is done with simple mixing without heating and hence these are referred to as 'cold-dispersible' (cold-processable) pearlescent concentrates. In addition to the benefits of ease of incorporation and saving of energy, the other significant advantage that is derived from these concentrates is that of the consistency in the pearlescent appearance in the

final personal and home care compositions. Several cold pearlescent concentrates are available commercially, for example, Sparkle series from Galaxy Surfactants Ltd, Mirasheen series from Rhodia (Solvay), Euperlan PK series from Cognis (BASF), Zoharpearl series from Zohar Dalia and Quickpearl series from Lubrizol (Table I).
TABLE I

Manufacturer Trade name Ingredients

Rhodia -Solvay Mirasheen CP 920 Ethylene glycol distearate, Laureth-7, Coamidopropyl betaine, Sodium cocoamphoacetate, Sodium laureth sulfate, DMDM Hydantoin
Rhodia -Solvay Mirasheen STARK Ethylene glycol distearate Sod laureth sulphate, Cocoamidopropyl betaine
Cognis-BASF EUPERLAN PK 1200 Ethylene glycol distearate Coco-glucoside,
Cognis-BASF EUPERLAN PK 4000 Ethylene glycol distearate, Laureth-4, Cocamidopropyl betaine
Cognis-BASF EUPERLAN PK 710 BENZ Ethylene glycol distearate Sodium laureth sulfate, Cocamide MEA,
Cognis-BASF EUPERLAN PK 710 Ethylene glycol distearate Sodium laureth sulfate, Cocamide MEA
Cognis-BASF EUPERLAN PK 771 Ethylene glycol distearate, Sodium laureth sulfate, Cocamide MEA, Laureth-10
Cognis-BASF EUPERLAN PK 771 BENZ Ethylene glycol distearate, Sodium laureth sulfate, Cocamide MEA, Laureth-10
Cognis-BASF EUPERLAN PK 810 Ethylene glycol distearate, Sodium laureth sulfate, Cocamide MEA, Laureth-10
Cognis-BASF EUPERLAN PK 900 BENZ -W PEG- 3 stearate, Sodium laureth sulfate

Lubrizol Quickpearl™ 810 Ethylene glycol distearate, Sodium laureth sulfate, Cocamide MEA, Laureth-10
Lubrizol Quickpearl™ I Ethylene glycol distearate, Sodium lauryl sulfate
Lubrizol Quickpearl™ II Ethylene glycol distearate Sod laureth sulphate
Lubrizol Quickpearl™ PK3 Pearlizing Agent Ethylene glycol distearate, Laureth-4, Cocamidopropyl betaine
Typically, all cold-dispersible pearlescent concentrates (TABLE I) are made of three components a) pearly waxes b) surfactants and c) water. Waxes are emulsified with surfactants in aqueous medium and then are gradually allowed to crystallize in the surfactant environment. This process results in a stable uniform dispersion of platelet like structures that reflect light. The examples of waxes are glycol esters of stearic acid or other fatty esters (Formula I) and alkanolamides (Formula II). The surfactants that are used to disperse the pearly waxes are from all categories, namely, anionic, cationic, amphoteric, zwitterionic and non-ionic (US Pat No 5,560,873, 5,711,899, 6,147,124, & 7,578,995).
A quick look at commercial products and the patented literature reveals that the most commonly used ingredients for free-flowing cold-dispersible pearlescent concentrates are 1) ethylene glycol stearates 2) alkanolamides 3) sodium laureth sulphate (anionic surfactant) 4) cocoamido propyl betaine (zwitterionic surfactant) 5) ethylene oxide adducts of fatty alcohols (non-ionic surfactant) and preservatives (TABLE I).
Alkanolamides are synthesized by reacting fatty acids or their esters with monoethanol amine or diethanol amine to give corresponding alkyl monoethanolamides or alkyl diethanol amides. The alkanol amides, particularly, alkyl diethanol amides have been reported to generate carcinogenic nitrosoamines (US pat 5,560,873) due to residual free diethanol amine. For example, cocodiethanol amide

(Cocamide DEA) has significant amount of residual diethanol amine that can generate the carcinogenic nitrosoamine after coming in contact with other personal care ingredient that are capable of nitrosating the secondary amine. Cocamide DEA has been listed as cancer causing substance by the Office of Environmental Health Hazard Assessment of California state government, USA (California prop 65 list). Personal care formulators have become very wary of using Cocamide MEA (cocomonoethanol amide) since monoethanol amine used in the manufacture can have diethanol amine as an impurity since there is no way of synthesizing monoethanol amine exclusively and selectively without generation of diethanol amine.
Alkyl sulphates and alkyl ether sulphates are known for being harsh on both skin and hair. In fact sodium lauryl sulphate is taken as a standard irritant for irritancy measurement. Sodium lauryl ether sulphate (SLES) is made by sulphating ethoxylated lauryl alcohol of varying degree of ethylene oxide (0.5 to 3.0 moles of EO per one mole of fatty alcohol). Any grade of SLES that is available in the market contains significant amount of sodium lauryl sulphate (more than 20 %) that is an established irritant to human skin and mucosa. In the class of anionic surfactants 'sulphates' in general are known to be the highest irritants compared to 'sulphonates or sulphosuccinates'. While doing the cleansing job, alkyl ether sulphates strip away the proteins and lipids of cuticle of hair and stratum corneum of skin. Skin's moisture regulation mechanism is seriously affected due to adverse action of harsh surfactants on the proteins and lipids of upper layers of stratum corneum. Products 'without sulphates' have been launched that do less or no damage to hair. An example of this is L'Oreal's 'sulphate-free' 'Everpure' range of shampoos. Unilever's Dove range of body washes uses mild surfactants like cocoyl isethionate and sodium glycinate to reduce damaging effect of harsh surfactants like fatty alchohol ether sulphates (Nutrium technology, A novel glycinate-based body wash, K.P.

Ananthapadmanabhan et al., Journal of Clinical Aesthetic Dermatology 23-30, 6(6), 2013).
Ethylene glycol stearates, mono or di, are the main pearlizing waxes that are used in these concentrates and these are by far the largest active component in the cold-dispersible, free-flowing pearlescent concentrates that are either available commercially or reported in literature. This is because these are the substances that crystallize and impart pearly effect to the final composition. Ethylene glycol stearates are made by esterifying stearic/palmitic acids with ethylene glycol. Though fatty acids are largely obtained from vegetable oils, mainly palm and coconut oil, ethylene glycol (mono ethylene glycol, MEG) has its origin in a petrochemical, ethylene oxide. Besides MEG's petrochemical origin, the more worrisome fact is its toxicity. The major impurity, diethylene glycol (DEG) in MEG is a serious concern. Ethylene glycol is listed by American Association of Poison Control Center that keeps track of fatalities due to ethylene glycol. Ethylene glycol is a teratogen in animals and hence suspected to be teratogen in human being. (Hazardous Substance Fact Sheet on Ethylene Glycol, New Jersey Department of Health, USA. Monoethylene glycol is on the Special Health Hazard Substance List) This means it is a huge concern for the environment and the ecology. All personal care and home care products like body-washes and hand-washes go to environment after the usage and are biodegraded to give ethylene glycol from the corresponding pearly distearates. The impurity, diethylene glycol (DEG) in mono ethylene glycol is another poison. US code of federal regulations allow no more than 0.2 % of DEG in polyethylene glycols used as food/drug or toothpaste additive. DEG is well-known poison and epidemiology is full of records of human deaths. In summary, in addition to being a petrochemical derived glycol, MEG is quite toxic due to impurities like DEG and by itself MEG is a danger to the environment due to its teratogenicity. There has been one commercial product by Cognis-BASF. (Care chemicals division, Technical Data Sheet, EUPERLAN® GREEN) by the trade name Euperlan Green seems to be the only

example wherein ethylene glycol stearate has been replaced by stearyl citrate and the non-ionic surfactant of lauryl glucoside is used to disperse the pearly wax. This was done with the intention of avoiding ethylene oxide (EO) based products. However, ethylene oxide is a gas at room temperature and does not remain in the product and it does not have to be necessarily sourced from petrochemical origin since ethylene oxide by molasses route (fermentation of molasses, green and sustainable route) is commercially available. Hence ethylene glycol (www.indiaglycol.com) made by bio-route is available. The tech bulletin of Eupearlan Green talks about product being ethylene oxide free. Ethylene oxide is available by sustainable bio-process. Thus, EO can be derived from petro-chemical route or bio (fermentation) route, however, the ethylene glycol made from either route is the concern due to its toxicity to the environment.
Cocoamidopropyl betaine, CAPB is popular zwitterionic surfactant and very commonly used in pearlescent concentrates. It is manufactured from methyl ester of fatty acid or from fatty acids by reacting with N, N-dimethyl propyl diamine to get the corresponding amido amine that is subsequently quaternized by monochloroacetic acid into final zwitterionic surfactant. Though it is one of the most economical zwitterionic surfactant it is impossible to manufacture CAPB that would be totally devoid of traces of free N, N-dimethyl propyl diamine and monochloroacetic acid. Both starting materials that are used in the manufacture of CAPB are quite toxic (DIN safety data sheet on N, N-dimethyl propyl diamine, BASF, 1989). It is a skin-corrosive (Category IB) and skin sensitizing (Category 1). The other trace level impurity in CAPB is highly acidic and corrosive monochloro acetic acid. It LD50 is just 76 mg/kg and is reported to penetrate though skin and mucous membrane.
A survey of commercially available cold pearlescent concentrates reveals that use of parabens, formaldehyde releasers and isothiazolinones. To move away from the

controversial substances some manufacturers adopted different strategy of maintaining the acidic pH and with acids as preservatives.
In some marketed pearly concentrates very acidic pH is maintained and the same are preserved with acids like benzoic acid or formic acid. For example Euperlan 771 is preserved with benzoic acid. Euperlan PK4000, Euperlan PK810 are preserved with formic acid. Similarly, MackPeari TTE and MacPearl SSO special are preserved with formic acid. Euperlan Green is preserved by maintaining very acidic pH of 3 to 4. Euperlan PK771 and Mirasheen Star K and Mirasheen Star NB are preserved with isothiazolinones. Mirasheen CP 820/G and Mirasheen A-220 are preserved with. DMDM hydantoin.
It should be noted these pearlescent concentrates are for personal care products and formic acid is never used as preservative for end personal care formulation and not the pH kept so acidic. Formic acid is corrosive to skin and the main constituent of ant venom. It is very reactive molecule and can react with a variety of functionality of other ingredients. Formaldehyde and formic acid are the metabolites that are responsible for optic nerve damage due to methanol. Its mutagenic properties and damage to kidneys in human due to exposure are reported.
Isothiazolinones, both analogs, methyl and chloro, (commercial trade name Kathon CG by Rohm and Haas) are quite toxic and hence they are allowed at a few ppm level and only in rinse-off products. They are not used in any leave-on products. Japan's regulatory body does not permit its usage. DMDM hydantoin, another antimicrobial prevalent in some of the pearlescent concentrates is a well-known formaldehyde releaser.
In view of this prevalent use of these established toxic antimicrobials (parabens, isothiazolinones, formaldehyde releasers, halogenated compounds, phenolic

molecules) it is very essential to preserve these pearlescent concentrates with something very effective but at the same time safe and eco-friendly.
US patent 5,560,873 discloses pearlizing concentrates that address the above mentioned issues to some extent. It teaches use of mild surfactants like sodium cocoyl isethionate and sodium cocoyl TV-methyl taurate in place the harsh alkyl ether sulphates. The pearlescent blends of this patent are expected to be mild on skin since they do not contain fatty alcohol ether sulphates. However, this patent does not report or refer to any literature or include data to suggest any synergy in terms of mildness arising out of in any particular combination of O-acyl isethionate and N-acyl taurate.
It also teaches creating pearlescent concentrates without carcinogenic alkanolamides, since the concentrates are used in personal care compositions. However, the examples cited in this patent employ ethylene glycol monostearate as the pearly wax, and cocoamidopropyl betaine, zwitterionic surfactant. Also, the preservatives listed in this patent application are the most controversial antimicrobials, the parabens and the formaldehyde releasers like imidazolidinyl urea. Parabens have been reported to have endocrine disrupting estrogenic activity and some deleterious effect on reproductive system. Formaldehyde is classified as category 3 CMR (carcinogenic, mutagenic and reproductive toxic). Also, released formaldehyde is a very reactive chemical (aldehyde functionality) capable of reacting with several personal care ingredients. Euperlan Green that obviates first four ingredients listed above, however, uses extreme acidity for the preservation. This extreme acidity comes from significant level of free acid. Also, the constituent members of Euperlan Green have significant characteristic odor.
Personal cleansing compositions of today have undergone revolutionary change. Recently, Tsaur et al. have revolutionized cleansing technology by inventing various combinations of mild surfactants. Synergistic combinations of O-acyl isethionate and

N-acyl glycinate / sarcosinate as 'super mild' surfactants are reported in the recent literature (US Patent Nos 8,268,767, 8,263,538, 8,114,824).
US Patent 8,263,538 teaches a combination of N-acyl amino acid surfactants, such as sodium N-cocoyl glycinates and sodium N-cocoyl sarcosinates, with amphoteric / zwitterionic surfactants for 'super mild' personal cleansing compositions that showed total irritation score using patch test methodology to be less than 75 % relative to 0.5 % aqueous sodium dodecyl sulphate solution.
Yet another recent patent by the same group (Tsaur et al US Patent 8,268,767) reports combinations of cleansing surfactants that not only cleanse, moisturize and deliver the benefit agents but are 'super mild' to skin. The said compositions comprise of both 0-acyl isethionates (Formula III, R4 = C7 to C21, R5 = H, methyl and M is a cation selected from Na+, K+, NH44+) and N-acyl amino acid surfactants (Formula IV, R4 = C7 to C21, R5 = H, methyl, M is a cation selected from NH44+, Na+ or K+) for liquid cleansers for both skin and hair.

The compositions are said to be 'super-mild' to skin when tested by standard patch test (in-vivo) method on human volunteers. The above mentioned patent also reveals exceptionally mild compositions that deliver the occlusive moisturizers very effectively. Similarly, US Patent 8,114,824 discloses compositions with O-acyl isethionates and N-acyl amino acid surfactants that could accommodate maximum

amount of moisturizer. In summary, the surfactant systems comprising of O-acyl isethionates and N-acyl amino acid surfactants as main ingredients have been reported to be very good cleansing systems that are 'super mild/gentle' on skin and excellent delivery vehicles for actives (benefit agents like emollients, silicones, triglycerides and petrolatum). The above mentioned patent teaches that the 'super mildness' and 'delivery benefits' are obtained due to the exceptional synergy exhibited by the combination of these two classes of mild surfactants, namely, O-acyl isethionates (Formula III) and N-acyl amino acid surfactants (Formula IV).
The commercial products using the 'super-mild' surfactant combinations (K.P. Ananthapadmanabhan et al., Journal of Clinical Aesthetic Dermatology 23-30, 6(6), 2013) are being made (Dove body wash, Nutrium MoistureTM technology by Unilever, Fine Fairness Cleanser by Johnson and Johnson).
Today's consumer and the manufacturers of personal care products are conscious about the deleterious effects of chemicals that are used in formulations. Conscious efforts are being made to use the safest ingredients that are also eco-friendly and sustainable. Personal care formulators are formulating cleansing systems with 'super-mild' surfactants (for cleansing face, body and hair). Consciously, toxic ingredients are being avoided and this includes the anti-microbial preservatives. The adjectives like 'sulphate-free', 'paraben free' and 'alkanolamide free' are getting popular. Certainly, to improve the aesthetics of such carefully formulated products, the formulators need a cold-dispersible pearlizing concentrate that is not just made from safe and non-toxic substances but it should be completely eco-friendly and sustainable. Currently, no such cold-dispersible pearlizing concentrate is available.
Thus, there is need for a cold-dispersible pearlescent concentrate that would be devoid of all controversial substances including 1) harsh surfactants like alkyl sulphates /alkyl ether sulphates 2) alkanol amides based on monoethanol amine and

diethanol amine 3) pearly waxes based on ethylene glycol 4) amidobetaines and 5) preservatives systems containing parabens, formaldehyde releasers, and isothiazolinones.
OBJECTS OF INVENTION
i) It is an objective of the present invention to develop a cold-dispersible pearlescent concentrates made by avoiding all controversial ingredients such as harsh surfactants, carcinogenic alkanol amides, eco-toxic pearly waxes, amidobetaines and antimicrobials.
ii) It is another objective of the present invention to develop a cold-dispersible pearlescent concentrate that is based on mild, non-toxic and eco-friendly ingredients.
iii) It is also an objective of this invention to develop a cold-dispersible pearlescent concentrate using 'mild surfactants'.
iv) Another object of the invention is to develop a cold-dispersible pearlescent concentrate using fatty acid esters of 1,3-propane diol as pearl wax.
v) It is yet another object of the present invention to develop a process for manufacturing cold-dispersible pearlescent concentrate.
SUMMARY OF INVENTION
In the first aspect, the present invention is directed to aqueous, free flowing cold-dispersible pearlescent concentrates, comprising; 1) Combination of 'super-mild' surfactants

2) Esters of 1,3-propane diol as pearl wax
3) N-acyl glycines
4) Preservative system comprising phenoxy ethanol, capryloyl glycine and undecylenoyl glycine.
Thus, the present invention discloses free-flowing, aqueous cold-dispersible pearlizing concentrates that are made by avoiding all controversial ingredients such as harsh surfactants, carcinogenic alkanol amides, non-ecofriendly pearly waxes, amidobetaines and antimicrobials.
The cold-dispersible, aqueous pearlescent concentrates of the present invention comprise of
a) 10 to 20 % by weight of mild surfactants selected from O-acyl isethionates (Formula III) and iV-acyl amino acid surfactants (Formula IV)

wherein, R4 is selected from C7 to C21saturated or unsaturated alkyl group, R5 is H or methyl and M is a cation selected from Na+, K+, NH4 + ;
b) 15 to 30 % by weight of pearlizing wax selected from 1, 3-propane diol esters of fatty acids (Formula V);


wherein, R6 is selected fromCo to C21 saturated or unsaturated alkyl group, R7 is H or
R6CO;
c) 2 to 6 % by weight of N-acyl glycine Formula VI;

wherein, R8 is selected fromC7 to C21 saturated or unsaturated alkyl group; and
d) 0.8 to 1.2 % by weight of preservative system consisting of phenoxy ethanol, capryloyl glycine and undecylenoyl glycine.
All percentages and ratios herein are on weight percent basis unless otherwise stated. The term 'super-mild' surfactants herein refer to O-acyl isethionates and N-acyl amino acid surfactants and their combination thereof.
The term 'cold' as utilized herein refers to the ability of the concentrate to be added without heating the personal and home care products.

In the second aspect, the present invention is directed to personal care compositions containing the cold-dispersibie pearlizer concentrate of the present invention.
DETAILED DESCRIPTION OF INVENTION
The present invention relates to a cold-dispersible pearlescent concentrate that is based on safe, mild, non-toxic and eco-friendly ingredients. The pearlescent compositions of the patent application are based on established 'super-mild' and completely biodegradable surfactants system, the combination of 0-acyl isethionates (Formula III) and N-acyl amino acid surfactants (Formula IV), eco-friendly pearly wax made from vegetable oil derived fatty acids and corn derived 1,3 -propane diol, and non-toxic, non-controversial preservatives.
In the first aspect, the present invention is directed to aqueous, cold-dispersible pearlescent concentrates, comprising;
1) Combination of 'super-mild' surfactants
2) Esters of 1,3-propane diol as pearl wax
3) N-acyl glycines
4) Preservative system comprising phenoxy ethanol, capryloyl glycine and undecylenoyl glycine.
The present invention discloses free flowing, aqueous, cold-dispersible pearlizing concentrates that are made by avoiding all controversial ingredients such as harsh surfactants, carcinogenic alkanol amides, non-ecofriendly pearly waxes, amidobeatines and antimicrobials.
The aqueous pearlescent concentrates of the present invention comprise of
a) 10 to 20 % by weight of mild surfactants selected from O-acyl isethionates
(Formula III) and iV-acyl amino acid surfactants (Formula IV);


wherein, R4 is selected fromC7 to C21 saturated or unsaturated alkyl group, R5 is H or methyl and M is a cation selected from Na+, K+, NH4 +;
b) 15 to 30 % by weight of pearlizing wax selected from 1,3 propane diol esters of fatty acids (Formula V);

wherein, R$is selected fromC13 to C21 saturated or unsaturated alkyl group, R7 is H or R6CO;
c) 2 to 6 % by weight of N-acyl glycine (Formula VI).


wherein, R8 is selected fromC7 to C21 saturated or unsaturated alkyl group; and
d) 0.8 to 1.2 % by weight of preservative system consisting of phenoxy ethanol, capryloyl glycine and undecylenoyl glycine.
In the second aspect, the present invention is directed to personal care compositions containing the cold-dispersible pearlizer concentrate of the present invention. In the present patent application the aqueous, cold-pearlizing concentrate is prepared by emulsifying the pearlizing wax by a combination of mild surfactants that have been established as 'super-mild' surfactants. The cold-dispersible pearlescent concentrates of the present invention are made by dispersing pearly wax, stearates of 1, 3-propane diol (Formula V) using 10 to 20 % by weight of mild surfactants selected from O-acyl isethionates (Formula III) and N-acyl amino acid surfactants (Formula IV, R4 = C7 to C21, R5 = H, methyl, M is a cation selected from NH4+, Na+ or K+).
In a preferred embodiment, the ratio of O-acyl isethionates and N-acyl amino acid surfactants is from 2.0:0.5 to 0.5:2.0.
O-Acyl isethionates (Formula III) can be selected from sodium cocoyl isethionate or sodium lauroyl isethionate. Commercially, the isethionates are available in solid form (powder, needles or granules) with a minimum 80 % anionic active matter. N-acyl amino acid surfactants (Formula IV) can be selected from sodium lauroyl glycinate, sodium cocoyl glycinate, sodium lauroyl sarcosinate or sodium cocoyl sarcosinate, which are available as aqueous solutions or in powder form. The preferred blends of 'super-mild' surfactants are O-acyl isethionates and N-acyl glycinates and the most preferred combination of super-mild surfactants is sodium N-cocoyl glycinate and sodium O-cocoyl isethionate in the ratio of 2:1. The blend of O-acyl isethionates and

N-acyl glycinates used in the present invention are prepared by the process reported in Indian Patent Application number 1669/MUM/2013/ by Koshti et al
The pearlizing wax of this patent application is selected from mono or di ester of 1,3-propane diol and fatty acids (Formula V). The alkyl chain can be selected from C12 to C22 for either mono or disters of propane diol. The preferred pearly wax of this patent application is diester of fatty acids and most preferred wax is 1,3-proane diol distearate (CAS No 75537-28-9). The fatty acids needed for preparing the esters are derived from vegetable oils like coconut oil or palm kernel oil. The vegetable oils with higher degree of unsaturation can be hydrogenated so that unsaturated alkyl chains of oleic, linoleic and linolenic or erucic acids can be converted to the corresponding saturated analogs of stearic acid or behenic acid. The stearic acid that is used for the preparation of distearates of 1,3-propane diol is from commercially available grades that are mixtures of stearic and palmitic acid, with stearic content being 40 to 80 %. Pure palmitic or stearic acid can also be used to make the pearly waxes. 1,3-Propane diol is made by fermentation of corn syrup. 1,3-Propane diol made from renewable vegetable source is commercially available under the trade name of Zemea® propane diol (CAS 504-63-2) offered by DuPont-Tate & Lyle. This diol, a personal care ingredient, is completely non-toxic and is used as humectants. Thus, the diesters of 1,3-propane diol, the pearly waxes of the present patent application are completely biodegradable and their degradation products, namely, fatty acids and propane diol, are completely eco-friendly unlike ethylene glycol of glycol stearates.
The non-ionic surfactant, employed in the pearlescent concentrates of the present invention is N-acyl glycine (Formula VI). This surfactant in its acid form is made by acidifying the aqueous solutions of commercially available sodium N-acyl glycinates and phase separating the N-acyl glycine (the acid form, forms upper layer) from the aqueous acidic layer. The N-acyl glycines can be further washed with water in hot condition to remove the mineral acidity. Depending upon the length of the alkyl chain

on nitrogen, N-acyl glycine can be a liquid or a solid. In the examples of this patent application, N-cocoyl glycine with alkyl chain distribution of C8 to C16 was predominantly used. It is the same alkyl chain distribution of sodium cocoyl glycinate (CAS No. 90387-74-9). N-acyl sarcosines made in similar way can also be used in place of N-acyl glycine (US pat 2,729,657).
In a preferred embodiment, 2-6% N-acyl glycine is used, preferably 4-6%.
The cocoyl cut alkyl chain distribution for N-cocoyl glycinate and N-cocoyl glycine is given in TABLE n.
TABLE II: Carbon chain distribution of Cocoyl group

Carbon chain Range
c8 4-12%
C10 4-14%
C12 59 -65 %
C14 14 -24 %
Cl6 1-8%
Cl8 0.5 to 12 %
It is obvious to those with ordinary skilled in the art that alkyl group R8 is selected from one alkyl chain or mixture of alkyl chains and can be selected from carbon atoms ranging from C8 to C20.
The pearlescent concentrates of the present application are preserved by a three component blend which is a mixture of three components, namely, phenoxy ethanol (CAS No 122-9-6, EC No: 204-589-7), capryloyl glycine (CAS Nol4246-53-8) and

undecylenoyl glycine (CAS No 54301-26-7, EINECS No 427-430-5) in the ratio of 8:1:1 by weight.
Capryloyl glycine, also known as N-octanoyl glycine, is a well-known skin purifier/protector. Commercially, it is available as Lipacide C8G from SEPPIC, France. It is active against most of the natural resident micro-flora that reside on human skin and is used in anti-acne, antiperspirant and deodorant preparations.
Undecylenoyl glycine is reported to possess anti-acne and anti-dandruff activity (EP 0983055131). It is commercially available as Lipacide UG and is used as dermopurifier.
2-Phenoxy ethanol occurs in nature (Chinese Green Tea) and has been consumed by human race for centuries. It is a gentle antimicrobial and is preferred in preserving vaccines that contain very labile proteins. 2-Phenoxy ethanol is active against Gram negative bacteria (Cosmetic and Drug Preservation, Principles and Practice, Vol I, Ed Jon Kabara, Marcel Dekker).
The preservative blend comprising 2-phenoxy ethanol, N-octanoyl glycine and N-undecylenoyl glycine in the ratio of 8:1:1 has been used at 1.2 % level in Examples 1, 2 & 3 and 1 % in shampoo, body wash and face-wash formulations of Examples 4, 5 and 6.
These preserved pearlescent concentrates from Examples 1, 2 and 3 have been

challenge- tested with initial levels of inoculation of 108 to 1010 cfu / ml as per CTFA~ guideline (Evaluation of preservatives to protect cosmetics' by D. Orth in Cosmetics and Toiletries, March 91).

As per CTFA guideline, on completing 28 days period of incubation, the concentrated blends of Example 1, 2 and 3 passed the challenge test. The test organisms that have been used in challenge test are Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Candida albicans and Aspergillus niger. Thus, the preservative combination used in pearlescent concentrates of the present invention are made up of very 'safe and non-toxic' personal care ingredients and are free from parabens, formaldehyde donors, phenolic compounds, halogenated molecules and quaternary ammonium molecules.
The process
The process of manufacture of cold-dispersible pearlescent concentrate of this patent application involves
1) Emulsification of pearly wax using combination of mild surfactants in a specified ratio that are established as 'super-mild' surfactants and a non-ionic surfactant in water at 75 to 80°C. The pearly wax of this patent application is a stearate ester of 1,3-propane diol and the non-ionic surfactant is N-acyl glycine.
2) The emulsified mass is gradually cooled to 45°C over 2 to 4 hours under gentle agitation. The antimicrobial preservatives are added at this stage and the whole mass is held at the same temperature for 12 to 24 hours. It is allowed to cool further to room temperature and pH is adjusted to 6.5 to 7.5 (if necessary) with citric acid and desired solids content of the pearly concentrate are adjusted (Example 1, 2 and 3). The viscosity of these pearlescent blends ranges between 3,000 to 20,000 cps and the solids content is kept minimum 35 % by weight. The particle size distribution is between 5 to 15 μm (D50) when measured using Malvern particle size analyzer (Mastersizer 2000E).

The pearlescent concentrate can be stored at a temperature of from about 20°C to about 40°C for six months without any significant change in physical properties.
The aqueous, cold-dispersible pearlescent concentrates of the present invention can be specifically formulated into a wide variety of personal care and home care products. These products can be formulated by one skilled in the art utilizing conventional methods of preparation. The pearlizing concentrate imparts a high luster, nacreous look to body-wash, face-wash and shampoo (Example 4, 5 and 6). Generally, the personal or home care products can be made by merely mixing the personal or home care products together with the concentrate at room temperature.
Advantages of the invention
1) The compositions of this patent application are based on established 'super-mild' surfactants combination of O-acyl isethionates, N-acyl amino acid surfactants, particularly, glycinates (K.P. Ananthapadmanabhan et al, Journal of Clinical Aesthetic Dermatology 23-30, 6(6)).
2) These compositions are free from fatty alcohol ether sulphates (SLES) that are harsh on skin (strip away both lipids and proteins of stratum corneum) and thereby alter the skin function. In the same manner, the sulphates cause damage to hair cuticle as well. In addition, SLES contains cancer causing 1,4-dioxane too.
3) The cold-dispersible pearlescent concentrates of the present invention use stearate esters of 1, 3-propane diol. 1, 3-Propane diol, unlike ethylene glycol (MEG is derived from petrochemical and is eco-toxic, proven teratogen in animals and possible teratogen for human. The impurity, DEG that can be present in MEG is highly poisonous) is derived from corn syrup by fermentation technology. Thus, 1, 3-

propane diol derived from renewable source is non-toxic substance and is used as humectant by cosmetic formulators.
4) The cold-dispersible pearlescent concentrates of the present invention do not use
any controversial ingredient like alkanol amides, ethylene glycol based esters and
alkyl amido propyl betaines.
5) It avoids usage of controversial, estrogeinic, carcinogenic, incompatible
antimicrobials e.g. parabens or formaldehyde releasers, phenolic, halogenated and
quaternary ammonium type of molecules.
6) The compositions of this patent application are preserved with a blend of phenoxy ethanol, capryloyl glycine and undecylenoyl glycine; all are established, commercially available and approved personal care ingredients.
7) The ingredients of the pearlescent concentrates of this patent application make the final compositions, cold-processable, mild on skin, completely safe (toxicologically), and finally eco-friendly compared to what is available in market currently. This makes the compositions of his patent applications completely sustainable from every aspect.
EXAMPLES
The present invention is now described by way of working on limiting illustrative examples. The detail of the invention provided in the following examples is given by the way of illustration only and should not be construed to limit the scope of the present invention.
Fatty acid, stearic acid was sourced from Palm Oleo SDN BHD, Malaysia. Biobased Zemea propane diol was sourced from DuPont Tate & Lyle Bio-Products, Loudon,

USA. Preservative blend was available with Galaxy Surfactants Ltd, Navi Mumbai, India, under the trade name of Galguard NK4 which is a combination of 2-phenoxy ethanol, capryloyl glycine and undecylenoyl glycine in the ratio of 8:1:1 by weight.
Preparation of 1,3-propane diol distearate:
The pearly wax 1,3-propane diol distearate was prepared by reacting two gmol of stearic acid and one gmol of 1,3-propane diol under the catalytic influence of sulphonic acid catalyst (0.02 mole %) at 130-140°C . The water of esterification was removed continuously with a steady purging of nitrogen throughout the progress of reaction. The reaction was monitored on the basis of acid value of the reaction mass and was continued till the acid value of < 10 was attained. The product was obtained as white flakes, with saponification value of 195 and melting point of 52- 57°C.
Preparation of cocoyl glycine:
Cocoyl glycine was prepared from sodium cocoyl glycinate sourced from Galaxy Surfactants Ltd, Navi Mumbai. It is available with a trade name of Galsoft SCG. The 30 % aqueous solution of sodium cocoyl glycinate is acidified with 10 % sulphuric acid. After attaining the pH of 1.0, the separated solid, cocoyl glycine was filtered and washed with cold water to get rid of the acidity of mineral acid. It was dried and then used for creating the cold pearlescent concentrates (Examples 1, 2 and 3) of the present invention.

Example 1: Preparation of cold-dispersible nearlizing blend using 'super-mild' surfactants in 2:1 ratio: Sodium cocovl elvcinate : sodium cocovl isethionate :: 2:1 and 1,3-propane diol distearate.

Ingredients Qty (g)
Sodium cocoyl glycinate (30 % aq. solution) 352
Sodium cocoyl isethionate (85 % active) 56
Cocoyl glycine 60
1,3-Propane diol distearate 200
Water 315
Citric acid pH adjustment (6 - 7)
Preservative : Galgurad NK 4
(2-phenoxy ethanol, capryloyl glycine and
undecylenoyl glycine in the ratio of (8:1:1) 12
Weight of total batch 1000
To a stirred solution of sodium cocoyl glycinate (352 g of 30 % aqueous solution, 24 % active with 5 % sodium chloride) and sodium cocoyl isethionate (56 g, 85% active) in water (237 mL), was added cocoyl glycine (60 g) and the resulting mixture was heated to 80°C for 15 minutes. To this mixture at 80°C, 1, 3-propane diol distearate (200 g) was added and stirred for additional 30 minutes. The resultant emulsion was then slowly cooled to 45°C over two hours with gentle stirring. To this dispersion, water (78 mL) was added and mass was cooled to room temperature under gentle stirring over a period of two hours. pH was adjusted to 6 to 7 using citric acid. It was preserved with a 1.2 % blend comprising 2-phenoxy ethanol, capryloyl glycine and undecylenoyl glycine in the ratio of (8:1:1) Analysis:
• Solids content: 42.7%
• Viscosity at 25 °C Brookfield LV 4. rpm 12 : 13000 cos

• pH (10 % dispersion): 6.4
• Sodium chloride content: 2 %
Example 2 : Preparation of pearlizing biend using 'super-mild' surfactants in 2:1 ratio : sodium cocoyl sarcosinate : sodium cocoyl isethionate :: 2: land 13-propane diol distearate

Ingredients Qty(g)
Sodium cocoyl sarcosinate (30 % aq. Solution, 24 % active) 352
Sodium cocoyi isethionate 85 % 56
Cocoyl glycine 60
1,3-Propane diol distearate 200
Water 315
Citric acid pH adjustment (6- -7)
Preservative :Galgurad NK 4
(2-phenoxy ethanol, capryloyl glycine and
undecylenoyl glycine in the ratio of (8:1:1) 12
Weight of total batch 1000
To a stirred solution of sodium cocoyl sarcosinate (352 g of 30% aqueous solution, 24 % active with 5 % sodium chloride) and sodium cocoyl isethionate (56 g, 85 % active) in water (237 mL), was added cocoyl glycine (60 g) and the resulting mixture was heated to 80°C for 15 minutes. To this mixture at 80°C, 1, 3-propane diol distearate (200 g) was added and stirred for additional 30 minutes. The resultant emulsion was then slowly cooled to 45°C over two hours with gentle stirring. To this dispersion, water (78 mL) was added and mass was cooled to room temperature under gentle stirring over a period of two hours. pH was adjusted to 6 to 7 using citric acid. It was preserved with a 1.2 % blend comprising 2-phenoxy ethanol, capryloyl glycine and undecylenoyl glycine in the ratio of (8:1:1)

Analysis:
• Solids content: 42.7 %
• Viscosity at 25 °C Brookfield LV 4, rpm 12 : 10000 cps
• pH (10 % dispersion): 6.4
• Sodium chloride content: 2 %
Example 3: Preparation of cold-dispersible pearlizing blend using 'super-mild' surfactants in 1:1 ratio: sodium cocovl glvcinate : sodium cocovl isethionate :: 1:1 and 1,3-propane diol distearate.

Ingredients Qty (g)

Sodium cocoyl glycinate (30 % aq. Solution) 262
Sodium cocoyl isethionate (85 % active) 74
Cocoyl glycine 30
1,3-Propane diol distearate 200
Water 422
Citric acid pH adjustment (6 - 7)
Preservative : Galguard NK 4
(2-phenoxy ethanol, capryloyl glycine and
undecylenoyl glycine in the ratio of (8:1:1) 12
Weight of total batch 1000
To a stirred solution of sodium cocoyl glycinate (262 g of 30 % aqueous solution, 24 % active with 5 % sodium chloride) and sodium cocoyl isethionate (74 g, 85 % active) in water (264 mL), was added cocoyl glycine (30 g) and the resulting mixture was heated to 80°C for 15 minutes. To this mixture at 80°C, 1, 3-propane diol distearate (200 g) was added and stirred for additional 30 minutes. The resultant emulsion was then slowly cooled to 45°C over two hours with gentle stirring. To this

dispersion, water (158 mL) was added and mass was cooled to room temperature under gentle stirring over a period of two hours. pH was adjusted to 6 to 7 using citric acid. It was preserved with a 1.2 % blend comprising 2-phenoxy ethanol, capryloyl glycine and undecylenoyl glycine in the ratio of (8:1:1). Analysis:
• Solids content: 38.3%
• Viscosity at 25 °C Brookfield LV 4, rpm 12 : 5000 cps
• pH (10% dispersion): 6.9
• Sodium chloride content: 1.5 % Example 4 : 'Sulfate Free' Shampoo

Ingredients %(w/w)
Phase A
Deionized water To make 100.0
Polyquaternium 10 0.2
PEG-120 Methyl Glucose Trioleate (&) Propanediol (Glucamate VLT) 4
Phase B
Sodium lauroyl glycinate (24%) 28
Sodium cocoyl taurate (40 %) 22
Cocoamidopropyl betaine (29 %) 5
Phase C
Amodimethicone 2
Polyquaternium 7 2
Pearlescent concentrate of Example 1 5
EDTA Na2 salt 0.1
Phenoxy ethanol, capryloyl glycine, and undecylenoyl glycine (8:1:1) 1

Disperse Polyquaternium 10 and Glucamate VLT in water separately. Add phase B to phase A and mix until homogeneous. Add phase C, stir until uniform. Adjust pH of the final formulation to 5.8 - 6.0 with 50 % citric acid solution in water. Blend in fragrance and color.
Example 5: 'Sulfate-Free' Body Wash

Ingredients % (w/w)
Phase A
Deionized water To make 100.0
Acrylate copolymer (Aqua SF-1) 3
Phase B
Sodium cocoyl glycinate (30 %) 10
Alkyl polyglucoside (C8/C10) 10
Sodium cocoyl taurate (40 %) 15
Cocamidopropyl betaine (29 %) 5
Phase C
Dimethicone 2
Polyquaternium - 7 2
Hydrolyzed wheat protein 1
Pearlescent concentrate of Example 1 4
EDTA disodium salt 0.1
Phenoxy ethanol, capryloyl glycine, and undecylenoyl glycine (8:1:1) 1
Disperse acrylate copolymer (Aqua SF 1) in water. Add phase A to Phase B and mix until homogeneous. Finally add phase C, stir until uniform. Adjust pH of the final formulation with 50 % citric acid aqueous solution. Blend in fragrance and color.

Example 6: Mild Face Wash

Ingredients % (w/w)
Phase A
Deionized water To make 100.0
Hydroxy ethyl cellulose 1.00
Phase B
Disodium laureth sulfosuccinate 15.00
Cocamidopropyl betaine 15.00
Sodium lauroyl sarcosinate 10.00
PEG-7 glyceryl cocoate glycerin 2%
Glycerin 5.00
Phase C
Hydrolyzed barley protein 01.00
Pearlescent concentrate of Example 1 05.00
EDTA disodium salt 00.10
Phenoxy ethanol, capryloyl glycine, and undecylenoyl glycine (8:1:1) 01.00
Disperse hydroxyethyl cellulose in water. Add phase B & C to phase A, stir until uniform. Adjust pH of the final formulation with 50 % citric acid to 5.8-5.9. Blend fragrance and color.

We claim
1) A free flowing cold-dispersible, aqueous pearlizirig concentrate comprising of
a) 10 to 20 % by weight of mild surfactants selected from 0-acyl isethionates (Formula III) and N-acyl amino acid surfactants (Formula IV);

wherein, R4 iS selected fromC7 to C21 saturated or unsaturated alkyl group, R5 is H or methyl and M is a cation selected from Na+, K+, NH4+ ;
b) 15 to 30 % by weight of pearlizing wax selected from 1,3 propane diol esters of fatty acids (Formula V);

wherein, R6 is selected from C13 to C21 saturated or unsaturated alkyl group, R7 is H or R6CO;
c) 2 to 6 % by weight of N-acyl glycine (Formula VI);


wherein, R8 is selected fromC7 to C21 saturated or unsaturated alkyl group; and
d) 0.8 to 1.2 % by weight of preservative system consisting of phenoxy ethanol, capryloyl glycine and undecylenoyl glycine.
2) A pearlescent concentrate as defined in claim la), wherein the ratio of O-acyl isethionates and N-acyl amino acid surfactants is from 2.0:0.5 to 0.5:2.0.
3) A free flowing, aqueous, cold-dispersible pearlizing concentrate of claim 1 comprising

a) combination of sodium N-cocoyl glycinate and sodium cocoyl isethionate in the ratio of 2 :1,10 % by weight;
b) 1,3-propane diol distearate, 20 % w/w;
c) cocoyl glycine, 5 % by weight; and
d) preserved with 1.2 % by weight of blend of phenoxy ethanol, capryloyl glycine, undecylenoyl glycine in the ratio of 8:1:1 by weight.
4) The Process for preparing free flowing, aqueous, cold-dispersible pearlizing
concentrate comprising the steps
(i) Emulsification of pearly wax (Formula V) by intimately mixing with 'super-mild' surfactants selected from O-acyl isethionates (Formula III) and

N-acyl amino acid surfactants (Formula IV) and a non-ionic surfactant (Formula VI) in water at 75 to 80 °C;
(ii) Cooling the emulsified mass to 45°C over 2 to 4 hours under gentle agitation;
(iii) Adding the preservatives to step (ii);
(iv) Holding the concentrate prepared in step (iii) at 45 °C - 50 °C for 12-24 hours; (v) Cooling the concentrate to room temperature.
5) A pearlescent concentrate according to claim 1, wherein the solid content is minimum 35% by weight.
6) A pearlescent concentrate according to claim 1, wherein the viscosity is between 3,000 to 20,000 cps.
7) A pearlescent concentrate according to claim 1 having D50 particle size of 5-15 fim.
8) Personal care compositions containing about 2 to 10 % by weight of aqueous, cold-dispersible, cold processable pearlescent concentrates of claim 1.