Description:The said application is Divisional Application for earlier filed Parent Patent Application No. IN202221032607 dated 07/12/2022, titled, “SYNERGISTIC AGROCHEMICAL INSECTICIDAL COMPOSITION”.

Technical Field of the Invention:
The present invention relates to a synergistic insecticidal composition for improving crop/plant health. More particularly, the present invention relates to a synergistic insecticidal composition comprising; (a) Cyantraniliprole; (b) at least one insecticide selected from Tolfenpyrad and Fipronil; alongwith agrochemically acceptable adjuvant, for effective management of insect-pest complex on variety of crops/fruits/vegetables.

The present invention further relates to a method of effective control of variety of insects/pest that damages various crops, fruits and vegetables by applying synergistic insecticidal composition comprising; (a) Cyantraniliprole; (b) at least one insecticide selected from Tolfenpyrad and Fipronil; along with agrochemically acceptable adjuvant, thereby improving crop health and increasing crop yield.

The present invention also relates to a process for preparation of said synergistic insecticidal composition.

Background and Prior art of the Invention:
Insects are a big threat to crops as they consume plant foliage, roots and stems, which can make these plant parts unsuitable for eating or other use. They damage aerial plant parts such as leaves, flowers, fruits, twigs, stems, branches etc., as well as ground plant parts such as seeds, roots, tuber etc., resulting into considerable yield loss.

Insecticides are of chemical and biological origin substances used to mitigate or kill insects of one or more species. They are used in agricultural, horticulture, forestry and garden sectors for controlling pests but they have other uses as well, such as eliminating insects that spread diseases. Insecticides are also used in homes and offices to control vectors, such as mosquitoes and ticks that are involved in spreading human and animal diseases.
Before insecticides gained widespread use, a significant portion of the crops grown was consumed by insects and led to regular losses. While there are some biological controls in the environment, such as natural predators or parasites that attack the insects which feed on crops, however, there was little to no control over these factors. The use of insecticides is necessary to provide the best crop protection to combat these pests. The crop protection that insecticides provide has played a big role in helping agriculture, especially when it comes to increasing yield.

There is a need for insecticides in almost all commercial agriculture requirements. The wide and frequent use of insecticide in commercial agriculture has enabled an enormous increase in crop yields and product quality which ultimately increased the ease to farmers in term of economic advantage as well as ease of farming activities.

Treating the crops and plants with insecticides as foliar application, soil applications and seed treatments helps to reduce damage from insects and related insecticidal diseases. The advantage of treating seeds or other plant propagation material with insecticides is the improvement in germination rates, improved plant health and increased yield.

However, in a single or solo administration of insecticide, the activity is very limited thereby disease control is narrow and there is always chances of run of deterrents. The main concern with the repeated use of single insecticide in solo formulations is the development of resistance by the insects/pests for that particular insecticide and at the end one has to apply more concentrated formulation of the insecticide. The high amount of insecticide may result in the toxicity to human beings as well as have bad effects on the environment. To reduce the risk of development of resistant strains, mixtures of different active compounds are nowadays conventionally employed for controlling harmful insects or pests. It is possible to ensure successful control over a relatively long period of time by combining active compounds having different mechanisms of action.

There is also a requirement of a stable insecticidal composition combining more than one active ingredient that has a synergistic effect and thereby reduce the need for multiple individual applications while providing better insecticidal activity. Further requirement also includes a combination product having synergistic effect which can be extensively applied in the field of agricultural for controlling wide spectra of insect which also exhibits good physical and chemical stability.

The combinations of insecticides are often used to facilitate disease control, to broaden spectrum of control and to retard resistance development.

Indian Patent No. IN330819 (IN201621017888) relates to novel liquid formulation comprising synergistic bioactive amounts of Tolfenpyrad and Bifenthrin along with one or more inactive excipients and process for preparing said formulation. IN’819 discloses and claims combination of Tolfenpyrad and Bifenthrin, however, in general, Bifenthrin does not provide an instant killing of insect. Exposed insects take longer time period to die from the effects of Bifenthrin. Hence, use of Bifenthrin is limited for controlling the insect attacks that are damaging fruit-bearing plants, vegetables or flowers. Further, the liquid formulation disclosed and claimed in IN’819 is specifically a suspoemulsion. In such formulation, the active ingredients are highly loaded thereby chances of only partial solubility of actives in water or oil phases, which resulting stability issues during long term storage.

Chinese Patent Publication No. CN104286005 relates to an efficient insecticide composition comprising fenpropathrin, biopesticide-lepimectin, dinotefuran and tolfenpyard used for killing agricultural pests. CN’005 discloses insecticidal composition comprises different group of insecticides viz., pyrethroid (fenpropathrin), biopesticide (lepimectin), neonicotinoid (dinotefuran) and pyrazole (tolfenpyard), however, application of combination of such broad spectrum insecticidal composition is limited only to control lepidopterous insects, homoptera sucking pest and mite pest.

Chinese Patent Publication No. CN102599169 relates to an insecticidal composition containing tolfenpyrad and chlorantranilipore as a first active component and dimethacarb as a second active component for preventing the diseases caused by diamondback moth larvae in Lepidoptera, thrips in Thysanoptera, rice stem borers, leaf folders, cotton bollworms. The composition disclosed and claimed in CN’169 is a binary combination comprises tolfenpyrad and chlorantranilipore OR tolfenpyrad and dimethacarb. In general, chlorantraniliprole (CTRP) is highly active against chewing pest insects (for e.g. locusts, grasshoppers, caterpillars, and beetles) but less effective against sucking pests (for e.g. aphids, leafhoppers, thrips, whitefly, flies, bugs, and mites). Thus, CTPR is effective only against certain group/class of insects thereby resulting into limited control over the targeted pests. Dimethacarb is a carbamate, however, carbamates are banned in many countries because of its hazardous effects to human beings.

Chinese Patent Publication No. CN104397007 relates to a high efficiency pesticide composition comprises of flubendiamide, lepimectin, dinotefuran and tolfenpyrad used for killing agriculture insects. CN’007 discloses insecticidal composition comprises different classes of insecticides viz., ryanoid (flubendiamide), biopesticide (lepimectin), neonicotinoid (dinotefuran) and pyrazole (tolfenpyard), however, application of combination of such broad spectrum insecticidal composition is limited only for controlling lepidopterous insects, homoptera sucking pest and mite pest.

Thus there is still a need for new advantageous combinations to provide a variety of options to best satisfy plant infestation control needs with higher uptake of active ingredient with reduced toxicity and enhanced efficacy. There is also a need for a synergistic combination which can provide broad spectrum control while having reduced rate of application and dose and yet provide enhanced insect control efficacy resulting in enhanced plant/crop yield.

In a view to provide a solution to aforementioned needs, the inventors of the present invention have come up with synergistic insecticidal composition comprising combination of systemic and contact insecticide; wherein, present synergistic composition act as protectant against insect related diseases. Since the present synergistic composition comprises broad spectrum insecticides, it also acts on variety of insects/pests. Further the mode of action of each component in the present synergistic insecticidal composition is different hence there is no fear of resistance development or cross resistance under multiple spray schedules.

Object of the Invention:
In accordance with the above, it is an object of the present invention to provide a synergistic insecticidal composition comprising; (a) Cyantraniliprole; (b) at least one insecticide selected from Tolfenpyrad and Fipronil alongwith agrochemically acceptable adjuvant, for effective management of insect-pest complex and for effective controlling of wide variety and large spectrum of insects/pests that damage various varieties of plants/crops, fruits and vegetables.

Another object of the present invention is to provide a process for preparation of said synergistic insecticidal composition.

Yet another object of the present invention is to provide a synergistic insecticidal composition that kill resistant insect pests, which are normally not controlled by other insecticides.

Yet another object of the present invention is to provide a synergistic insecticidal composition that is totally safe with no phytotoxic effect on crops, fruits and vegetables.

Yet another object of the present invention is to provide a synergistic insecticidal composition that is stable at higher temperature conditions without affecting content and suspensibility of actives.

Yet another object of the present invention is to provide a synergistic insecticidal composition with increased shelf life and storage stability.

Summary of the invention:
In accordance with the above objectives, in an aspect the present invention describes a synergistic insecticidal composition comprising;
(a) Cyantraniliprole;
(b) at least one insecticide selected from Tolfenpyrad and Fipronil;
alongwith agrochemically acceptable adjuvant, for effective management of insect-pest complex and for effective controlling of wide variety and large spectrum of insects/pests that damage various varieties of plants/crops, fruits and vegetables.

In another aspect, the insecticide Cyantraniliprole is present in an amount of 0.5% to 35% by weight of the total composition.

In another aspect, the insecticide Tolfenpyrad is present in an amount of 0.5% to 35% by weight of the total composition

In another aspect, the insecticide Fipronil is present in an amount of 2.5% to 70% by weight of the total composition.

In another aspect, the agrochemically acceptable adjuvants of the present composition are selected from dispersing/co-dispersing agents, wetting/co-wetting agents, anti-foaming agents/deformer, anti-freezing agents, coating agents/polymers, binder/co-binders, stabilizers, emulsifiers, rheology modifiers, preservatives, solvent/co-solvents and fillers, and these excipients are present in an amount of 0.01% to 15% by weight of the total composition.

In yet another aspect, the synergistic insecticidal composition of the present invention is formulated in different dosage forms such as Suspension concentrate (SC), Capsule suspension (CS), Zeon concentrate (ZC) - mixed formulation of CS and SC, Water dispersible granules (WG or WDG), Wettable powder (WP), Oil-dispersion (OD), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable concentrate for seed treatment (FS), Granules (GR), Controlled (Slow or Fast) release granules (CR), Jambo balls or bags (bags in water soluble pouch), Solution for foliar spray (LS), Micro-emulsion (ME), Oil miscible flowable concentrate (OF), Oil miscible liquid (OL), Oil dispersible powder (OP), Suspension concentrate for direct application (SD), Suspo-emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Spreading oil (SO), Water soluble powder (SP), Water soluble tablet (ST), Ultra-low volume (ULV) Tablet (TB), Aerosol (AE), Ultra-low volume (ULV) suspension (SU), Ultra-low volume liquid (UL), Water dispersible powder (WDP), Water dispersible tablet (WT), a mixed formulation of CS and SE (ZE), a mixed formulation of CS and EW (ZW).

In yet another aspect, the present invention describes the process for preparation of different types of composition; wherein, these compositions are prepared by mixing appropriate quantity of active ingredients with agrochemically acceptable excipients under certain appropriate conditions.

In yet another aspect, the present invention describes a method of effectively controlling/reducing wide variety of insects/pests, improving crop health and increasing crop yield by applying synergistic insecticidal composition of the present invention on different varieties of plants/crops, fruits and vegetables.

In yet another aspect, the present invention describes a synergistic insecticidal composition with combination of insecticides that demonstrates high and faster insects/pests control efficacy, no phytotoxicity, reduced crop protection cost and reduced environmental load.

In yet another aspect, the present invention describes a synergistic insecticidal composition which is active against all life stages of major crop/plant insects/pests/pathogens.

In yet another aspect, the present invention describes a synergistic insecticidal composition that is stable at higher temperature conditions without affecting the suspensibility and content of actives, thereby increasing shelf life and storage stability of the composition.

In yet another aspect, the present invention describes a synergistic insecticidal composition that is capable of controlling a large spectrum of insects in a wide range of crops, especially the order of lepidoptera, hemiptera, coleoptera, diptera, hymenoptera, isopteran and is also effective against controlling soil pest, gram pod borer, stem borer, fruit borer, leaf roller, leaf folder, leaf miner, looper, hoppers, thrips, jassids, aphid and mites that damages variety of crops, fruits, vegetables and non-agronomic crops (other than field crops).

Detailed Description of the Invention:
The present invention will now be described in detail in connection with certain embodiments, so that various aspects thereof may be fully understood and appreciated.
The phrase “Synergistic” used herein, refer as the interaction of two or more active agents combined together and apply conjointly to produce an effect which is greater than the sum of their individual effects.

The phrase, "Effective amount" used herein refer as an amount of the active ingredient that when applied is sufficient to achieve a good level of control.

The present invention discloses a unique synergistic insecticidal composition comprising combination of systemic and contact insecticide. The active ingredient Cyantraniliprole is a systemic insecticide; Tolfenpyrad is a contact insecticide and Fipronil is a contact and systemic insecticide.

Mode of action of Insecticides:
Systemic insecticides: ‘Systemic insecticide’ means insecticide that is absorbed by and transported through plants and can render some or all of a plant toxic to insects that feed on plant tissue. These are the insecticides that become a part of the plant through systematic distribution. This type of insecticide is introduced into the soil to get it absorbed by the plant roots. Once the insecticide enters the roots, it moves to external areas such as leaves, fruits, twigs, and branches. It forms a layer on the plant surface area and acts as a poison to any insect that comes to chew the plant. Systemic insecticides have residual or long-term activity.

Contact insecticides: ‘Contact insecticide’ means insecticide that are designed to kill insects via contact with or ingestion of surface residues shortly after application. These are the insecticides that can penetrate the skin of the insects. They are toxic to insects when they come in contact with it. These types of insecticides act like bullets that aim only at a particular target to kill insects by its application. They can be of many kinds including naturally occurring compounds and synthetic organic compounds. Usually, household insect spray works like contact insecticides as it must directly hit the insect. Contact insecticides generally have little to no residual activity.

Thus, by combining the insecticides with different mode of action in a present synergistic composition, a control over a wide variety of insects/pest belongs to order lepidoptera, hemiptera, coleoptera, diptera, hymenoptera, isopteran and also over soil pest, gram pod borer, thrips, jassids, aphid and mites is highly possible.

Accordingly, in an embodiment, the present invention discloses a synergistic insecticidal composition comprising;
(a) Cyantraniliprole in an amount of 0.5% to 35%;
(b) at least one insecticide selected from Tolfenpyrad in an amount of 0.5% to 35% and Fipronil in an amount of 2.5% to 70%;
alongwith agrochemically acceptable adjuvant in an amount of 0.01% to 15% by weight of the total composition, for effective management of insect-pest complex and for effective controlling of wide variety and large spectrum of insects/pests that damage various varieties of plants/crops, fruits and vegetables.

In one preferred embodiment, the present invention discloses a synergistic insecticidal composition comprising;
(a) Cyantraniliprole in an amount of 0.5% to 35%; and
(b) Tolfenpyrad in an amount of 0.5% to 35%;
alongwith agrochemically acceptable adjuvant in an amount of 0.01% to 15% by weight of the total composition.

In another preferred embodiment, the present invention discloses a synergistic insecticidal composition comprising;
(a) Cyantraniliprole in an amount of .5% to 35%; and
(b) Fipronil in an amount of 2.5% to 70%;
alongwith agrochemically acceptable adjuvant in an amount of 0.01% to 15% by weight of the total composition.

The agrochemically acceptable adjuvants of the present synergistic insecticidal composition are selected from dispersing/co-dispersing agents, wetting/co-wetting agents, anti-foaming agents/deformer, anti-freezing agents, coating agents/polymers, binder/co-binders, stabilizers, emulsifiers, rheology modifiers, preservatives, solvent/co-solvents, fillers and the like.

Examples of dispersing/co-dispersing agents include but not limited to methyl methacrylate polymer, sodium lignosulfonates; sodium naphthalene sulphonate formaldehyde condensates; tristyryl phenol ethoxylate phosphate esters, polyarylphenyl ether phosphate, polyalkelene glycol ether, tristyrylphenol phosphate ester, tristyrylphenol ethoxylate amine salt of phosphate, tristyrylphenol phosphate amine salt, tristyrylphenol ethoxylated, tristyryl phenol ethylated, acrylic co-polymer, acrylate copolymer, acrylic copolymer, acrylic copolymer sodium salt, alkyl naphthalene sulfonate formaldehyde condensate, alkyl naphthalene sulfonate, naphthalene sulfonic acid, sodium alkyl naphthalene sulfonate blend, sodium polycarboxylate, sodium polyacrylate, ethoxylated oleyl cetyl alcohol, ethoxylated tristryl phenol sulphate, ethoxylated fatty alcohol, polymeric non-aqueous dispersing agent, polyoxyethylene isotridecanol, tristyrylphenol ethoxylate phosphate ester, sodium salt condensate with formaldehyde, aliphatic alcohol ethoxylates; alky ethoxylates; EO-PO block copolymers; and graft copolymers, either alone or mixtures thereof.

Examples of wetting/co-wetting agents include but not limited to block copolymer, alkoxylated alcohol, ethoxylated propoxylated alcohol, polyalkoxylated butyl ether, sodium alkyl sulphate, sodium lauryl sulphate, sodium alkyl benzene sulfonate, dioctyl sulfosuccinate, polyoxyethylene sorbitol hexaoleate, polyoxyethylene sorbitan monolaurate, polyoxyethylene polyoxypropylene sorbitan monolaurate, polyoxyethylene lauryl ether, tristyrylphenol ethoxylated, alkoxyleted alcohol, alkyl naphthalene sulfonate formaldehyde condensate, block copolymer, sodium dioctyl sulpho succinate, alkyl phenol ethoxylates and aliphatic alcohol ethoxylates, either alone or mixtures thereof.

Examples of anti-foaming agent/deformer include but not limited to siloxane polyalkyleneoxide, polydimethylsiloxane, silicon based agents, either alone or mixtures thereof.

Examples of anti-freezing agent include but not limited to glycol, polyethylene glycols, monoethylene glycol, glycerin, diethylene glycol, propane-1,2-diol, methoxy polyethylene glycols, polypropylene glycols, polybutylene glycols, glycerin and ethylene glycol, either alone or mixtures thereof.

Examples of coating agents/polymers include but not limited to Polymeric isocyanate, Polyol, Polyisocyanates, Polydiisocyanates, polymethylene polyphenylene isocyanate (PMPPI), hexamethylene diisocyanate (HMDI), Napthalene diisocyanate, Methylene bis-cyclohexylisocyanate, isophorone diisocyanate (IPDI) or 4,4' methylenebis (cyclohexyl isocyanate), polyphenylisocyanate, Polymethylene polyphenylisocyanate, Toluene diisocyanate, and/or trimers of HMDI or IPDI, Trimethyl hexamethylene, Xylene diisocyanate, Tetramethyl xylene diisocyanate, Hexahydrotoluylene diisocyanate (HTDI), methylene diphenyl diisocyanate (MDI) and blocked polyisocyanates either alone or mixtures thereof.

Examples of binders/co-binder include but not limited to aluminium silicate, precipitated silica, fumed silica, diatomaceous earth, glucose polymers: amylose and amylopectin, a branched form, lactose anhydrous, silicic acid, sodium lignosulonate, modified phyllosilicate, either alone or mixtures thereof.

Examples of stabilizers include but not limited to polysaccharides, carboxymethyl cellulose, bentonite clay, aluminum magnesium silicate, citric acid either alone or mixtures thereof.

Examples of emulsifiers include but not limited to calcium dodecyl benzene sulfonate, tristyryl phenyl ethoxylate, triglyceride, calcium alkyl benzen sulfonate, either alone or mixtures thereof.

Examples of rheology modifier include but not limited to polysaccharides, carboxymethyl cellulose, bentonite clay, aluminum magnesium silicate, either alone or mixtures thereof.

Examples of preservatives include but not limited to 1,2-benzisothiazolin-3-one, benzoic acid, sodium benzoate, propionic acid, sorbic acid, and sodium diacetate, either alone or mixture thereof.
Examples of solvent/co-solvent include but not limited to aromatic solvent, di-methyl formamide, N-methyl pyrrolidone, heavy aromatic naptha, solvent naphtha either alone or mixtures thereof.

Examples of filler include but not limited to water, DM water, silicon dioxide, china-clay, kaolin, talc, starch, urea formaldehyde resin, aluminium silicate, methylated oleic acid ester, methylated fatty acid ester, either alone or mixtures thereof.

In another embodiment, the synergistic insecticidal composition of the present invention is formulated in different formulation/composition types selected from Suspension concentrate (SC), Capsule suspension (CS), Zeon concentrate (ZC) - mixed formulation of CS and SC, Water dispersible granules (WG or WDG), Wettable powder (WP), Oil-dispersion (OD), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable concentrate for seed treatment (FS), Granules (GR), Controlled (Slow or Fast) release granules (CR), Jambo balls or bags (bags in water soluble pouch), Solution for foliar spray (LS), Micro-emulsion (ME), Oil miscible flowable concentrate (OF), Oil miscible liquid (OL), Oil dispersible powder (OP), Suspension concentrate for direct application (SD), Suspo-emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Spreading oil (SO), Water soluble powder (SP), Water soluble tablet (ST), Ultra-low volume (ULV) Tablet (TB), Aerosol (AE), Ultra-low volume (ULV) suspension (SU), Ultra-low volume liquid (UL), Water dispersible powder (WDP), Water dispersible tablet (WT), a mixed formulation of CS and SE (ZE), a mixed formulation of CS and EW (ZW).

In another preferred embodiment, the synergistic insecticidal composition of the present invention is formulated as Suspension concentrate (SC) or Zeon concentrate (ZC) [a mixed formulation of CS and SC] or Water dispersible granule (WDG or WG).

In another embodiment, the active ingredients used in the present synergistic insecticidal composition viz., Tolfenpyrad, Cyantraniliprole and Fipronil is having 95%, 92% and 92% purity, respectively.

In another embodiment, the present invention discloses the process for preparation of different composition types such as Suspension concentrate (SC) or Zeon concentrate (ZC) [a mixed formulation of CS and SC] or Water dispersible granule (WDG or WG); wherein these compositions prepared by mixing required quantity of active ingredients with agrochemically acceptable adjuvants under certain appropriate conditions. The process for preparation of each composition is provided in the example section.

In yet another preferred embodiment, the present invention discloses a method for effective controlling of wide variety and large spectrum of insects/pests that damage various varieties of plants/crops, fruits and vegetables by applying an effective amount of synergistic insecticidal composition comprising;
(a) Cyantraniliprole in an amount of 0.5% to 35%;
(b) at least one insecticide selected from Tolfenpyrad in an amount of 0.5% to 35% and Fipronil in an amount of 2.5% to 70%;
alongwith agrochemically acceptable adjuvant in an amount of 0.01% to 15% by weight of the total composition.

In yet another preferred embodiment, the present invention discloses a method for effective controlling of wide variety and large spectrum of insects/pests that damage various varieties of plants/crops, fruits and vegetables by applying an effective amount of synergistic insecticidal composition comprising;
(a) Cyantraniliprole in an amount of 0.5% to 35%; and
(b) Tolfenpyrad in an amount of 0.5% to 35%;
alongwith agrochemically acceptable adjuvant in an amount of 0.01% to 15% by weight of the total composition.

In yet another preferred embodiment, the present invention discloses a method for effective controlling of wide variety and large spectrum of insects/pests that damage various varieties of plants/crops, fruits and vegetables by applying an effective amount of synergistic insecticidal composition comprising;
(a) Cyantraniliprole in an amount of 0.5% to 35%; and
(b) Fipronil in an amount of 2.5% to 70%;
alongwith agrochemically acceptable adjuvant in an amount of 0.01% to 15% by weight of the total composition.

In yet another embodiment, the present invention discloses a method of effectively improving crop health and increasing crop yield of variety of crops, fruits and vegetables by applying an effective amount of synergistic insecticidal composition comprising;
(a) Cyantraniliprole in an amount of 0.5% to 35%;
(b) at least one insecticide selected from Tolfenpyrad in an amount of 0.5% to 35% and Fipronil in an amount of 2.5% to 70%;
alongwith agrochemically acceptable adjuvant in an amount of 0.01% to 15% by weight of the total composition.

In yet another embodiment, the present invention discloses a synergistic insecticidal composition comprising; (a) Cyantraniliprole; (b) at least one insecticide selected from Tolfenpyrad and Fipronil; alongwith agrochemically acceptable adjuvant; that is totally safe with no phytotoxic effect on crops, fruits and vegetables and is active against all life stages of major crop/plant insects/pests/pathogens.

In yet another embodiment, the present invention discloses a synergistic insecticidal composition comprising; (a) Cyantraniliprole; (b) at least one insecticide selected from Tolfenpyrad and Fipronil; alongwith agrochemically acceptable adjuvant; that is stable at higher temperature conditions without affecting the suspensibility and active ingredient content, thereby increasing shelf life and storage stability of composition.

In yet another embodiment, the present invention discloses a method of effectively controlling insects and pests from the order Lepidoptera, Hemiptera, Coleoptera, Diptera, Hymenoptera, and Isopteran by applying an effective amount of synergistic insecticidal composition comprising; (a) Cyantraniliprole; (b) at least one insecticide selected from Tolfenpyrad and Fipronil; alongwith agrochemically acceptable adjuvant.

The order lepidoptera include insect-pest such as Agrotis ypsilon, Alabama argillacea, Anticarsia gemmatalis, Cacoecia murinana, Capua reticulana, Chilo auricilius, Chilo infuscatellus, Chilo partellus, Chilo sacchariphagus, Chilo suppressalis, Choristoneura fumiferana, Choristoneura occidentalis, Cirphis unipuncta, Cnaphalocrocis medinalis, Cydia pomonella, Diatraea saccharalis, Dendrolimus pini, Diaphania nitidalis, Earias vittella, Earias insulana, Elasmopalpus lignosellus, Emmalocera depressella, Eupoecilia ambiguella, Evetria bou-liana, Exelastis atomosa, Feltia subterranea, Galleria mellonella, Grapholitha funebrana, Helicoverpa armigera, Helicoverpa virescens, Helicoverpa zea, Hellula undalis, Hibernia defoliaria, Hyphantria cunea, Hyponomeuta malinellus, Keiferia lycopersicella, Laphygma exigua, Leucinodes orbonalis, Leucoptera coffeella, Leucoptera scitella, Lithocol-letis blancardella, Lobesia botrana, Loxostege sticticalis, Lymantria dispar, Lymantria monacha, Lyonetia clerkella, Malacosoma neustria, Mamestra brassicae, Marasmia pantalis, Maruca vitrata, Maruca testulalis, Mythimna separata, Orgyia pseu-dotsugata, Ostrinia nubilalis, Panolis flammea, Pectinophora gossypiella, Peridroma saucia, Phalera bucephala, Phthorimaea operculella, Phyllocnistis citrella, Pieris bras-sicae, Plathypena scabra, Plutella xylostella, Pseudoplusia includens, Rhyacionia frus-trana, Scirpophaga excerptallis, Scirpophaga incertulas, Scirpophaga innotata, Scrobipalpula absoluta, Sesamia inferens, Sitotroga cerealella, Sparganothis pilleriana, Spilosoma obliqua, Spodoptera frugiperda, Spodoptera littoralis, Spodoptera litura, Thaumatopoea pityocampa, Tortrix viridana, Trichoplusiani, Tryporyza novella, Tuta absoluta and Zeiraphera Canadensis.

Hemiptera include insect-pest such as Acrosternum hilare, Acyrtho-siphon pisum, Acyrthosiphon onobrychis, Adelges laricis, Aleurolobus barodensis, Amrasca biguttula biguttula, Amrasca devastans, Amritodus atkinsoni, Aphidula nasturtii, Aphis fabae, Aphis forbesi, Aphis pomi, Aphis gossypii, Aphis crassivora, Aphis grossulariae, Aphis schneideri, Aphis spiraecola, Aphis sambuci, Aulacorthum solani, Bemisia argentifolii, Bemisia tabaci, Brachycaudus cardui, Brachy-caudus helichrysi, Brachycaudus persicae, Brachycaudus prunicola, Brevicoryne brassicae, Capitophorus horni, Cavalerius sweeti, Cerosipha gossypii, Ceratovacuna lanigera, Chaetosiphon fragaefolii, Cicadulina spp., Clavigralla gibbosa, Cryptomyzus ribis, Dreyfusia nordmannianae, Dreyfusia piceae, Dysdercus cingulatus, Dysaphis radicola, Dysaulacorthum pseudosolani, Dysaphis plantaginea, Dysaphis pyri, Empoasca fabae, Hyalopterus pruni, Hyperomyzus lactucae, Idioscopus spp. Leptoglossus phyllopus, Leptocorisa acuta, Lygus lineolaris, Lygus pratensis, Macrosiphum avenae, Macrosiphum euphorbiae, Ma-crosiphon rosae, Megoura viciae, Melanaphis pyrarius, Metopolophium dirhodum, My-zus persicae, Myzus ascalonicus, Myzus cerasi, Myzus varians, Nezara viridula, Nasonovia ribis-nigri, Nilaparvata lugens, Nephotettix virescens, Nephotettix nigropictus, Pemphigus bursarius, Perkinsiella saccharicida, Peregrinus maidis, Phorodon humuli, Planococcus spp., Pseudococcus spp., Pyrilla perpusilla, Psylla mail, Psylla piri, Rhopalomyzus ascalonicus, Rhopalosiphum maidis, Rhopalosi-phum padi, Rhopalosiphum insertum, Saccharicoccus sacchari, Sappaphis mala, Sappaphis mail, Schizaphis graminum, Schizoneura lanuginosa, Sitobion avenae, Sogatella furcifera, Trialeurodes vaporariorum, Toxoptera aurantiia, Psylla spp., Rhopalosiphum spp., Sitobion spp.

Coleoptera include insect-pest such as Adoretus bicolor, Agrilus sinuatus, Agriotes lineatus, Agriotes obscu-rus, Amphimallus solstitialis, Anisandrus dispar, Anthonomus grandis, Anthonomus pomorum, Anomala benghalensis, Aphthona euphoridae, Apogonia aerea, Athous haemorrhoidalis, Atomaria linearis, Blasto-phagus piniperda, Blitophaga undata, Bruchus rufimanus, Bruchus pisorum, Bruchus lentis, Byctiscus betulae, Cassida nebulosa, Cerotoma trifurcata, Cetonia aurata, Ceuthorrhynchus assimilis, Ceuthorrhynchus napi, Chaetocnema tibialis, Chiloloba acuta, Conoderus vespertinus, Crioceris asparagi, Ctenicera ssp., Diabrotica longicornis, Diabrotica semipunctata, Diabrotica punctata, Diabrotica speciosa, Diabrotica virgifera, Dicladispa armigera, Epila-chna varivestis, Epitrix hirtipennis, Eutinobothrus brasiliensis, Holotrichia bicolor, Holotrichia consanguinea, Holotrichia serrata, Hylobius abietis, Hypera brunneipennis, Hypera postica, Ips typographus, Lema bilineata, Lema melanopus, Leptinotarsa decemlineata, Lepidiota stigma, Limonius califomicus, Lissorhoptrus oryzophilus, Maladera indica, Melanotus communis, Meligethes aeneus, Melolontha hippocastani, Melolontha melolontha, Oberea brevis, Oulema oryzae, Ortiorrhynchus sulcatus, Otiorrhynchus ovatus, Phaedon cochleariae, Phyllobius pyri, Phyllotreta chrysocephala, Phyllophaga sp., Phyllopertha horticola, Phyllotreta nemorum, Phyllotreta striolata, Popillia japonica, Sitona lineatus and Sito-philus gran aria.

Diptera include insect-pest such as Atherigona orientalis, Calliphora vicina, Ceratitis capitata, Chrysomya bezziana, Chrysomya hominivorax, Chrysomya macellaria, Chrysops discalis, Chrysops silacea, Chrysops atlanticus, Cochliomyia hominivorax, Contarinia sorghicola Cordylobia anthropophaga, Culicoides furens, Culiseta inornata, Culiseta melanura, Dacus cucurbi-tae, Dacus oleae, Dasineura brassicae, Delia antique, Delia coarctata, Delia platura, Delia radicum, Dermatobia hominis, Fannia canicularis, Geomyza Tripunctata, Gaster-ophilus intestinalis, Glossina morsitans, Glossina palpalis, Glossina fuscipes, Glossina tachinoides, Haematobia irritans, Haplodiplosis equestris, Hippelates spp., Hylemyia platura, Hypoderma lineata, Leptoconops torrens, Liriomyza sativae, Liriomyza trifolii, Lucilia caprina, Lucilia cuprina, Lucilia sericata, Lycoria pectoralis, Mansonia titillanus, Mayetiola destructor, Melanagromyza obtuse, Muscina stabulans, Oestrus ovis, Ophiomyia phaseli, Opomyza florum, Orseolia oryzae, Oscinella frit, Pegomya hysocyami, Phorbia antiqua, Phorbia brassicae, Phor-bia coarctata, Phlebotomus argentipes, Psorophora columbiae, Psila rosae, Psoro-phora discolor, Prosimulium mixtum, Rhagoletis cerasi, Rhagoletis pomonella, Sar-cophaga haemorrhoidalis, Sarcophaga sp., Simulium vittatum, Stomoxys calcitrans, Tabanus bovinus, Tabanus atratus, Tabanus lineola, and Tabanus similis, Tipula ol-eracea, and Tipula paludosa.

Hymenoptera include insect-pest such as Athalia rosae, Atta cephalotes, Atta capiguara, Atta cephalotes, Atta laevigata, Atta robusta, Atta sexdens, Atta texana, Crematogaster spp., Hoplocampa minuta, Hoplocampa testudinea, Monomorium pha-raonis, Solenopsis geminata, Solenopsis invicta, Solenopsis richteri, Solenopsis xyloni, Pogonomyrmex barbatus, Pogonomyrmex californicus, Pheidole megacephala, Dasymutilla occidentalis, Bombus spp. Vespula squamosa, Paravespula vulgaris, Paravespula pennsylvanica, Paravespula germanica, Dolichovespula maculata, Vespa crabro, Polistes rubiginosa, Camponotus floridanus, and Linepithema humile.

Isopterans include insect-pest such as Calotermes flavicollis, Coptotermes formosanus, Heterotermes aureus, Leucotermes flavipes, Microtermes obesi, Odontotermes obesus, Reticulitermes flavipes, Termes natalensis, Cockroaches (Blattaria - Blattodea), e.g. Blattella germanica, Penplaneta americana, Periplaneta japonica and Blatta orientalis.

In yet another embodiment, the present invention discloses a method of effectively controlling insects and pests selected from gram pod borer, stem borer, fruit borer, leaf roller, leaf folder, leaf miner, looper, hoppers, thrips, jassids, aphid and mites by applying an effective amount of synergistic insecticidal composition comprising; (a) Cyantraniliprole; (b) at least one insecticide selected from Tolfenpyrad and Fipronil; alongwith agrochemically acceptable adjuvant, on variety of crops, fruits and vegetables.

In yet another embodiment, the present invention discloses a synergistic insecticidal composition that can be applied to any and all developmental stages of insect/pests, such as egg, larva, pupa, and adult.

The composition of present invention is effective for management of insects/pests feed on variety of crops, fruits and vegetables such as Cotton (Gossypium spp.), Paddy (Oryza sativa), Wheat (Triticum aestavum), Maize (Zea mays), Sugarcane (Saccharum officinarum), Soybean (Glycin max), Peanut (Arachis hypogaea), Mustard (Brassica juncea), Green gram (Vigna radiate), Black gram (Vigna mungo), Chickpea (Cicer aritinum), Redgram (Cajanus cajan), Brinjal (Solanum melongena), Cabbage (Brassica oleracea var. capitata), Cauliflower (Brassica oleracea var. botrytis), Okra (Abelmoschus esculentus), Onion (Allium cepa L.), Tomato (Solanum lycopersicun), Potato (Solanum tuberosum) , Chilly (Capsicum annum), Garlic (Allium sativum), Cucumber (Cucumis sativus), Muskmelons (Cucumis melo), Watermelon (Citrullus lanatus), Bottle gourd (Lagenaria siceraria), Apple (Melus domestica), Banana (Musa spp.), Citrus groups (Citrus spp.), Grape (Vitis vinifera), Mango (Mangifera indica), Pomegranate (Punica granatum), Tea (Camellia sinensis), Cumin (Cuminum cyminum), Fenugreek (Trigonella foenum-graecum), Fennel (Foeniculum vulgare), Coriander (Coriandrum sativum) and the like.

In yet another embodiment, the present invention discloses a synergistic insecticidal composition comprising; (a) Cyantraniliprole; (b) at least one insecticide selected from Tolfenpyrad and Fipronil; alongwith agrochemically acceptable adjuvant is useful for controlling insect/pests in field crops, veg and horticultural crops, plantation crop, industrial land crops, wood-plywood industry and building & construction sites.

The composition of present invention also provides a non-agronomic (other than field crops) application such as application on horticultural crops (e.g., greenhouse, nursery or ornamental plants not grown in a field), residential, commercial and industrial structures, turf (e.g., sod farm, pasture, golf course, lawn, sports field, etc.).

Examples:
The present invention is further illustrated by the following examples. The different aspects and embodiments of the present invention may become more apparent from the examples set forth herein below. These examples describe possible preferred embodiments for illustrative purposes only, but they do not limit the scope of the invention.

Example 1 to Example 2: Suspension Concentrate (SC) Compositions
Example 1: Tolfenpyrad 15% + Cyantraniliprole 7.5% SC Example 2: Fipronil 7.5% + Cyantraniliprole 7.5% SC
No. Ingredients % w/w % w/w Role / Function
1. Tolfenpyrad Technical
(95% purity) 15.00 - Active ingredient
2. Fipronil Technical
(92% purity) - 07.50 Active ingredient
3. Cyantraniliprole Technical
(92% purity) 07.50 07.50 Active ingredient
4. Tristyrylphenol phosphate ester 04.00 04.00 Dispersing agent
5. Alkoxylated alcohol 02.00 02.00 Wetting agent
6. Polydimethyl Siloxane 00.20 00.20 Anti-foaming Agent
7. 1,2-benziso thiazolin-3-one 00.10 00.10 Preservative
8. Propylene glycol 06.00 06.00 Anti-freezing Agent
9. Triglyceride 04.00 04.00 Emulsifier
10. Polysaccharide 00.27 00.27 Rheology modifier
11. Citric acid 00.10 00.10 Stabilizer
12. Water Q.S. Q.S. Filler
Total 100.00

Example 3: Process for Preparation of Suspension Concentrate (SC)
Step 1: Homogenized the required quantity (as mentioned in the above table) of anti-foaming agent and water followed by addition of gum powder with stirring till complete dissolution to obtain a gum solution (Gum Solution should be made 12-18 hour prior to use).
Step 2: Required quantity (as mentioned in the above table) of DM water, dispersing agent and wetting agent was added into the charged vessel followed by homogenization for 45-60 minutes using high shear homogenizer.
Step 3: Active ingredients and other remaining adjuvants viz., emulsifier, rheology modifier, stabilizer, preservative were added into the homogenized slurry of step 2 to obtain a uniform slurry which is ready for grinding.
Step 4: Before grinding half quantity of required antifoam agent was added and then material was subjected to three cycles of grinding in Dyno mill. The remaining half quantity of the antifoam was added along with anti-freezing agent after grinding process completes and before sampling process analysis.
Step 5: Finally gum solution as obtained in the step 1 was then added to obtain the Suspension Concentrate formulation.

Example 4 to Example 5: Zeon concentrate (ZC) Compositions
Example 4: Tolfenpyrad 15% + Cyantraniliprole 7.5% ZC Example 5: Fipronil 7.5% + Cyantraniliprole 7.5% ZC
No. Ingredients % w/w % w/w Role / Function
1. Tolfenpyrad Technical
(95% purity) 15.00 - Active ingredient
2. Fipronil Technical
(92% purity) - 07.50 Active ingredient
3. Cyantraniliprole Technical
(92% purity) 07.50 07.50 Active ingredient
4. Tristyrylphenol phosphate ester 04.00 04.00 Dispersing agent
5. Acrylic copolymer 01.10 01.10 Wetting cum dispersing
6. Polymeric Isocyanate 02.10 02.10 Monomer
7. Polyol 00.43 00.43 Monomer
8. Solvent naphtha 08.00 08.00 Solvent
9. Alkoxylated alcohol 02.00 02.00 Wetting agent
10. Polydimethyl Siloxane 00.20 00.20 Anti-foaming agent
11. 1,2-benziso thiazolin-3-one 00.10 00.10 Preser-vative
12. Propylene glycol 06.00 06.00 Anti-freezing agent
13. Triglyceride 04.00 04.00 Emulsifier
14. Polysaccharide 00.27 00.27 Rheology modifier
15. Citric acid 00.10 00.10 Stabilizer
16. Water Q.S. Q.S. Filler
Total 100.00

Example 6: Process for Preparation of Zeon concentrate (ZC)
Step 1: Preparing an organic phase by mixing required quantity (as mentioned in above table) of solvent with active ingredients viz., Tolfenpyrad. After addition of the active ingredients, add monomer and mix well. Apply the heating, if required.
Step 2: Adding water in a clean vessel fitted with stirrer. Mixing dispersing agent, wetting agent, and preservative with continuous stirring till homogeneous mass is obtain.
Step 3: Mixing solution of step 1 and step 2 with stirrer at 55°C to form a pre-mix slurry and passing this slurry through homogenizer @ of 5500 rpm feeding rate and collect into a clean vessel fitted with stirrer and maintain temperature 60°C under starring.
Step 4: Addition previously prepared amine compound solution with a fix rate in homogenized mass and stir the solution for 2 hrs. at 55°C temperature and cool. Add previously hydrated xanthan gum mix well to get a homogenous CS (capsule suspension).
Step 5: Mixing active ingredients viz., Fipronil and Cyantraniliprole with anti-foaming agent, anti-freezing agent, emulsifier, , stabilizer and DM water till homogeneous mass is formed and passing this mass through bead mill to achieve required particle size followed by adding rheology modifier and making it homogeneous to get SC (suspension concentration).
Step 6: Mixing both phases i.e. capsule suspension of step 4 and suspension concentration of step 5 together properly to get a final ZC composition.

Example 7: Water Dispersible Granule (WDG) Composition
Tolfenpyrad 15% + Cyantraniliprole 7.5% WDG
No. Ingredients % w/w Role / Function
1. Tolfenpyrad Technical (95% purity) 15.00 Active ingredient
2. Cyantraniliprole Technical (95% purity) 07.50 Active ingredient
3. Sodium alkyl benzene sulfonate 02.43 Wetting agent
4. Sodium alkyl sulphate 08.87 Wetting agent / Co-wetter
5. Alkyl naphthalene sulfonate 06.91 Dispersing agent / Co-dispersant
6. Precipitated silica 08.11 Binder / Co-binder
7. Polydimethyl siloxane 01.01 Anti-foaming agent
8. Aluminium silicate Q.S. Filler
Total 100.00

Example 8: Process for Preparation of Water Dispersible Granule (WDG)
Step 1: Required quantity (as mentioned in the above table) of wetting agent, dispersing agent, antifoaming agent, binder and active ingredients was mixed in a premixing blender for homogenization for a period of 30 minutes to obtain a pre-blended material.
Step 2: The pre-blended material as obtained in step 1 was blended through Jet mill/ air classifier mills followed by blending in post blender for a period of approx. 1.5 hour to obtain a homogeneous mixture.
Step 3: Required quantity of filler (QS) was then added to step 2 to make a dough. The dough was then passed through the extruder to obtain granules of required size.
Step 4: Wet granules as obtained were passed through the fluidised bed drier followed by grading using vibrating screens to obtain the water dispersible granules.

Bio-efficacy Studies:

Introduction:
Chilli, Capsicum annum (Linnaeus) is an important solanaceous vegetable crop widely used as a vegetable, spice, condiment, sauce, pickles and medicine. Chilli is integral and the most important ingredient in many different cuisines around the world as it adds pungency, taste, flavour and colour to the dishes. Today, it is unimaginable to think of Indian cuisine without hot spice and chilli. Indian chilli is world famous for two important commercial qualities, it is colour and pungency levels. Major chilli growing countries are India, China, Mexico, Thailand, Ethiopia, Uganda and Pakistan. India is the world’s largest producer, consumer and exporter of chilli with a 7.43 lakh ha area. The important chilli growing states are Andhra Pradesh, Maharashtra, Karnataka, Orissa, Gujarat and Tamil Nadu accounting for more than 70 per cent acreage of India (Anonymous, 2021). Growing chilli faces several major constraints like abiotic and biotic stresses. Among the biotic stresses, pest and disease infestation are also equally responsible for colossal losses. About 51 species of insects and 2 species of mites belonging to 27 families under 9 orders along with snail are known to damage chilli crops both in the nursery and main field (Anon., 2020). A major threat to chilli cultivation all over the world is its proneness to infestation by pest complexes, especially mites, thrips, fruit borers, whiteflies etc. at different stages of crop growth. Among the common insect pests of vegetables, fruits and ornamental crops, the thrips (Scirtothrips dorsalis, Thysanoptera: Thripidae) is a serious pest. The adults and nymphs infest tender leaves and derive their nutrients by penetrating the tissues of chilli plants. They lacerate the tender leaf surface resulting in typical leaf curl symptoms and a boat-like appearance, locally called “Kokadava” (Patel et al., 2009). Typical symptoms of thrips damage include silvering of leaf surface, linear thickening of leaf lamina, necrotic spots on leaves and tender fruits, fruit distortion, and early senescence of leaves. Severe infestation causes “chilli leaf curl” also known as “Murda disease”. Thrips are also the vector of chilli leaf curl virus disease (Anon., 2020). At present, many newer synthetic and botanical insecticide molecules are available in the market to manage chilli thrips. The bio-efficacy of these molecules needs to be studied for the registration in chilli crop. Based on the wider adaptability by the farmers, three insecticides namely Cyantraniliprole, Fipronil, and Tolfenpyrad were selected for the present study. Keeping these points in view, detailed investigations were undertaken.

Materials and Method:
All the 8 treatments of solo, tank mixtures, pre-mixtures and market samples with one control treatment were applied as a foliar spray, with the help of a knapsack sprayer (15-litre capacity). For deciding the quantity of spray fluid required, control plots were sprayed with water. Spray fluid was prepared by mixing a measured quantity of water and insecticides. All necessary care was taken to prevent the drift of insecticides to reach the adjacent plots. When the population of thrips crossed its ETL level (5 thrips/leaf), the first spray was given on 10th November 2022, the second spray was given at 20 days interval, i.e., on 30th November 2022 and the third spray was given after 20 days i.e. on 21st December, 2022. Observations were recorded visually during the early morning from three leaves (i.e. bottom, middle and top) of each of 5 randomly selected plants along with the fruit borers count, from a net plot of each treatment. A pre-spray observation was taken and subsequently, the observations were recorded at 3, 7, 10 and 14 days after spraying from each treatment. The data on the thrips and fruit borers population was analyzed by applying the Colby equation as mentioned in Table 1 for testing the significance of the treatment effect and drawing a valid conclusion.

Table 1:
The presence of a synergistic effect between two active ingredients is established with the aid of the Colby equation (se S. R. Colby, "Calculating Synergistic and Antagonistic Responses of Herbicide Combinations', Weeds, 1967, 15, 20-22):

The presence of a synergistic effect between three active ingredients is established with the aid of the Colby equation (se S. R. Colby, "Calculating Synergistic and Antagonistic Responses of Herbicide Combinations', Weeds, 1967, 15, 20-22):

Using the method of Colby, the presence of a synergistic interaction between three active ingredients is established by first calculating the predicted activity, p, of the mixture based on activities of the three components applied alone. If 'p' is lower than the experimentally established efficacy, Synergism has occurred. In the equation above, A is the insecticidal activity in percentage control of one component applied alone at rate x. The B term is the insecticidal activity in percentage control of the second component applied at rate y & similarly C is the corresponding value for the third component applied at rate z. The equation estimates p, the insecticidal activity of the mixture of A at rate X with B at rate y & C at rate z if their effects are strictly additive and no interaction has occurred.

Efficacy tested against following insect pests:
Crop/Plant Insect
Chilli Thrips (Scirtothrips dorsalis)
Chilli Fruit borer (Hellicoverpa armigera)

Example 9: Trial 1
Synergistic impact of Binary mixtures:
Synergistic insecticidal cummulative effect of three sprays of a combination of ‘Tolfenpyrad + Cyantraniliprole’ AND ‘Fipronil + Cyantraniliprole’ as binary mixtures against Thrips and Fruit borer of Chilli crop is tested. Yield impact against Scirtothrips dorsalis (Thrips) and Hellicoverpa armigera (Fruit borer) is also evaluated and results are provided in below Table 2.

Test Crop Chilli
Test Insect Thrips (Scirtothrips dorsalis)
Test Insect Fruit borer (Hellicoverpa armigera)

Table 2: Synergistic impact of Binary mixtures
Synergistic insecticidal cumulative effect of three sprays of a combination of Tolfenpyrad + Cyantraniliprole & Fipronil + Cyantraniliprole as binary mixtures against Thrips & Fruit borer of Chilli crop and yield impact.

Scirtothrips dorsalis - No. of thrips/leave & Hellicoverpa armigera - Number of Borers/ 3 plants
Treatments Tolfenpyrad Fipronil Cyantraniliprole
g a.i./ha
T1 120.0 0.0 0.0
T2 150.0 0.0 0.0
T3 180.0 0.0 0.0
T4 0.0 60.0 0.0
T5 0.0 75.0 0.0
T6 0.0 90.0 0.0
T7 0.0 0.0 60.0
T8 0.0 0.0 75.0
T9 0.0 0.0 90.0
T10 120.0 0.0 60.0
T11 150.0 0.0 75.0
T12 180.0 0.0 90.0
T13 0.0 60.0 60.0
T14 0.0 75.0 75.0
T15 0.0 90.0 90.0
T16 800ml/Ha Premix of Tolfenpyrad 15% + Cyantraniliprole 7.5% - SC (120 + 60 g a.i./ha respectively)
T17 800ml/Ha Premix of Tolfenpyrad 15% + Cyantraniliprole 7.5% - ZC (120+60 g a.i./ha respectively)
T18 800ml/Ha Premix of Tolfenpyrad 15%+ Cyantraniliprole 7.5% - WDG (120 + 60 g a.i./ha respectively)
T19 800ml/Ha Premix of Fipronil 7.5% + Cyantraniliprole 7.5% SC (60+60 g a.i./ha respectively)
T20 800ml/Ha Premix o Fipronil 7.5% + Cyantraniliprole 7.5% - ZC (60+60 g a.i./ha respectively)
T21 800ml/Ha market sample of Tolfenpyrad 15% + Fipronil 7.5% SC
T22 625ml/Ha market sample of Cyantraniliprole 7.3% + Diafenthiuron 36.4%-SC
T23 Untrated control (sprayed only water)

Cumulative effect of 3 sprays 14DAT of final spray against Thrips
Pooled over periods & Sprays Percent control of Thrips after 3rd Spray Expected % control of Thrips after 3rd Spray Difference (ODC-EDC) % Thrips control with Colby
T1 4.66 56.29 -
T2 4.33 59.38 -
T3 4.00 62.48 -
T4 4.50 57.79 -
T5 4.30 59.66 -
T6 4.00 62.48 -
T7 4.33 59.38 -
T8 4.00 62.48 -
T9 3.50 67.17 -
T10 1.66 84.43 82.24 2.18
T11 1.33 87.52 84.76 2.77
T12 0.87 91.84 87.68 4.16
T13 1.75 83.58 82.85 0.73
T14 1.50 85.93 84.86 1.06
T15 0.66 93.81 87.68 6.13
T16 1.33 87.52 82.24 5.28
T17 1.00 90.62 82.24 8.38
T18 1.00 90.62 82.24 8.38
T19 1.33 87.52 82.24 5.28
T20 1.50 85.93 82.24 3.69
T21 2.66 75.05 - -
T22 2.33 78.14 - -
T23 10.66 0.00 94.96 -

Cumulative effect of 3 sprays 14DAT of final spray against Fruit borer
Fruit borers cumulative no. of 3 sprays after 14 DAT Observed Borer Control % (OBC) Expected Borer Control % (EBC) Difference (OBC-EBC) % with Colby
T1 7.00 32.24 - -
T2 5.87 43.18 - -
T3 5.00 51.60 - -
T4 6.33 38.72 - -
T5 5.87 43.18 - -
T6 4.87 52.86 - -
T7 5.33 48.40 - -
T8 4.66 54.89 - -
T9 4.33 58.08 - -
T10 3.00 70.96 65.04 5.92
T11 2.50 75.80 74.37 1.43
T12 1.87 81.90 79.71 2.19
T13 2.87 72.22 68.38 3.83
T14 2.00 80.64 74.37 6.27
T15 1.66 83.93 80.24 3.69
T16 2.66 74.25 65.04 9.21
T17 2.33 77.44 65.04 12.41
T18 2.33 77.44 65.04 12.41
T19 2.00 80.64 65.04 15.60
T20 3.00 70.96 65.04 5.92
T21 4.00 61.28 - -
T22 3.66 64.57 - -
T23 10.33 0.00 - -

Yield impact qt/ha
Yield of Chilli fruits qt/ha Observed Yield increase over control Expected yield increase over control Difference (OYI-EYI)% with Colby equation
T1 74.66 26.82
T2 77 30.80
T3 81.33 38.15
T4 72.00 22.30 - -
T5 76.87 30.58 - -
T6 80.00 35.89 - -
T7 76.50 29.95 - -
T8 79.66 35.32 - -
T9 82.33 39.85 - -
T10 96.66 64.19 48.74 15.46
T11 100.33 70.43 55.24 15.19
T12 107.66 82.88 62.80 20.08
T13 94.33 60.23 45.57 14.66
T14 101.00 71.56 55.09 16.47
T15 108.33 84.02 61.44 22.58
T16 97.66 65.89 48.74 17.15
T17 100.33 70.43 48.74 21.69
T18 98.00 66.47 48.74 17.73
T19 97.00 64.77 48.74 16.03
T20 99.33 68.73 48.74 19.99
T21 88.66 50.60 - -
T22 84.33 43.25 - -
T23 58.87 - -

Observation: It is observed from above Table 2 that the Binary mixtures were found to be superior in controlling the fruit borers i.e. from 70.96% to 83.93% (T10 to T20) than solo products i.e. from 32.24% to 58.08% (T1 to T9) and marketed products i.e. 61.28% to 64.57% (T21 to T22).

It is also observed from above Table 2 that the Binary mixtures demonstrate higher yield i.e. from 94.33qt/ha to 108.33qt/ha (T10 to T20) than solo products i.e. from 72.00qt/ha to 82.33qt/ha (T1 to T9) and marketed products i.e. from 43.25qt/ha to 50.60qt/ha (T21 to T22).
, C , Claims:We Claim,

1. A synergistic insecticidal composition comprising:
(a) Cyantraniliprole in an amount of 0.5% to 35%;
(b) at least one insecticide selected from Tolfenpyrad in an amount of 0.5% to 35% and Fipronil in an amount of 2.5% to 70%;
alongwith agrochemically acceptable adjuvant in an amount of 0.01 to 15% by weight of total composition;
wherein said composition is formulated in the form of Suspension concentrate (SC) or Zeon concentrate (ZC) or Water dispersible granule (WDG).

2. The synergistic insecticidal composition as claimed in claim 1, wherein said agrochemically acceptable adjuvant is selected from dispersing/co-dispersing agents, wetting/co-wetting agents, anti-foaming agents/deformer, anti-freezing agents, coating agents/polymers, binder/co-binders, stabilizers, emulsifiers, rheology modifiers, preservatives, solvent/co-solvents and fillers.

3. The synergistic insecticidal composition as claimed in claim 2, wherein said dispersing/co-dispersing agent is selected from methyl methacrylate polymer, sodium lignosulfonates, tristyrylphenol phosphate ester, tristyrylphenol phosphate amine salt, tristyrylphenol ethoxylated, acrylate copolymer, acrylic copolymer, acrylic copolymer sodium salt, alkyl naphthalene sulfonate formaldehyde condensate, alkyl naphthalene sulfonate, sodium polyacrylate, polymeric non-aqueous dispersing agent, polyoxyethylene isotridecanol, tristyrylphenol ethoxylate phosphate ester, and mixtures thereof.

4. The synergistic insecticidal composition as claimed in claim 2, wherein said wetting/co- wetting agent is selected from alkoxylated alcohol, polyalkoxylated butyl ether, sodium alkyl sulphate, sodium alkyl benzene sulfonate, dioctyl sulfosuccinate, polyoxyethylene sorbitol hexaoleate, polyoxyethylene sorbitan monolaurate, polyoxyethylene polyoxypropylene sorbitan monolaurate, polyoxyethylene lauryl ether, tristyrylphenol ethoxylated, alkyl naphthalene sulfonate formaldehyde condensate, and mixtures thereof.

5. The synergistic insecticidal composition as claimed in claim 2, wherein said anti-foaming agents/deformer is selected from siloxane polyalkyleneoxide, polydimethylsiloxane, silicon based agents, and mixtures thereof.

6. The synergistic insecticidal composition as claimed in claim 2, wherein said anti-freezing agent is selected from polyethylene glycols, monoethylene glycol, propane-1,2-diol, polypropylene glycols, and mixtures thereof.

7. The synergistic insecticidal composition as claimed in claim 2, wherein said coating agent/polymer is selected from Polymeric isocyanate, Polyol, Polyisocyanates, Polydiisocyanates, polymethylene polyphenylene isocyanate (PMPPI), hexamethylene diisocyanate (HMDI), Toluene diisocyanate, and mixtures thereof.

8. The synergistic insecticidal composition as claimed in claim 2, wherein said binder/co-binder is selected from aluminium silicate, precipitated silica, fumed silica, diatomaceous earth, glucose polymers: amylose and amylopectin, a branched form, lactose anhydrous, silicic acid, sodium lignosulonate, modified phyllosilicate, and mixtures thereof.

9. The synergistic insecticidal composition as claimed in claim 2, wherein said stabilizer is selected from polysaccharides, bentonite clay, citric acid, and mixtures thereof.

10. The synergistic insecticidal composition as claimed in claim 2, wherein said emulsifier is selected from calcium dodecyl benzene sulfonate, triglyceride, and mixtures thereof.

11. The synergistic insecticidal composition as claimed in claim 2, wherein said rheology modifier is selected from polysaccharides, carboxymethyl cellulose, aluminum magnesium silicate, and mixtures thereof.

12. The synergistic insecticidal composition as claimed in claim 2, wherein said preservative is selected from 1,2-benzisothiazolin-3-one, sodium benzoate, sodium diacetate, and mixture thereof.

13. The synergistic insecticidal composition as claimed in claim 2, wherein said solvent/co-solvent is selected from di-methyl formamide, N-methyl pyrrolidone, solvent naphtha, and mixtures thereof.

14. The synergistic insecticidal composition as claimed in claim 2, wherein said filler is selected from water, china-clay, kaolin, talc, aluminium silicate, methylated oleic acid ester, methylated fatty acid ester, and mixtures thereof.

Dated this 17th day of July 2024