FORM-2
THE PATENTS ACT, 1970
(39 of 1970)
&
THE PATENTS RULES, 2003
COMPLETE SPECIFICATION
(See Section 10 and Rule 13)
SYNERGISTIC COMBINATION OF A FUNGICIDE AND AN ACARICIDE
INDOFIL INDUSTRIES LIMITED
an Indian Company
of KALPATARU SQUARE,
4th FLOOR, KONDIVITA ROAD,
OFF: ANDHERI-KURLA ROAD,
ANDHERI (E) MUMBAI-400,059 INDIA
Inventors:
1. RAO JAYPRAKASH GOPALKRISHNA
2. BHARAMBE SHAILENDRA MITHARAM
3. KAKADE SHARAD MAHADEO
THE FOLLOWING SPECIFICATION PARTICULARLY DESCRIBES THE INVENTION AND THE MANNER IN WHICH IT IS TO BE PERFORMED.

FIELD OF DISCLOSURE:
The present disclosure relates to a synergistic biocidal composition. The present disclosure particularly relates to a synergistic biocidal composition containing a fungicide and an acaricide. The present disclosure also relates to a process for preparing a synergistic biocidal composition.
BACKGROUND AND PRIOR ART:
The dithiocarbamate fungicide, Mancozeb is described in GB996264; US3379610 (23.04.1968) and can be used to control many fungal diseases in a wide range of field crops, fruit, nuts, vegetables, ornamentals, etc. More frequent uses include control of early and late blights {Phytophthora infestans and Alternarai solani) of potatoes and tomatoes; downy mildew (Plasmopara viticola) and black rot {Guignardia bidwellii) of vines; downy mildew (Pseudoperonospora cubensis) of cucurbits; scab {Venturia inaequalis) of apples; Sigatoka (Mycosphaerella spp.) of bananas; and melanose {Diaporthe citri) of citrus. Used for foliar application or as a seed treatment.
MANCOZEB

IUPAC NAME: Manganese ethylenebis(dithiocarbamate) (polymeric) complex with
zinc salt
CAS NUMBER; {8018-01-7) (based on Composition)
MOLECULAR WEIGHT: 271.2
MOLECULAR FORMULA: [C4H6MnN2S4]xZny
Form: Greyish-yellow, free flowing powder, with a slight odour of hydrogen sulphide

Water solubility: In water 6.2 ppm (pH 7.5, 25 oC). Insoluble in most organic solvents; dissolves in solutions of powerful chelating agents but cannot be recovered from them.
Uses: Control of many fungal diseases in a wide range of field crops, fruit, nuts, vegetables, ornamentals, etc. More frequent uses include control of early and late blights {Phytophthora infestans and Alternaria solcmi) of potatoes and tomatoes; downy mildew {Plasmopara viticola) and black rot {Guignardia bidwellii) of vines; downy mildew {Pseudoperonospora cubensis) of cucurbits; scab (Venturia inaequalis) of apple; sigatoka (Mycosphaerella spp.) of banana and melanose {Diaporthe citri) of citrus. Typical application rates are 1500-2000 g/ha. Used for foliar application or as a seed treatment.
Mode of Action: Fungicide with protective action. Reacts with, and inactivates the sulfhydryl groups of amino acids and enzymes of fungal cells, resulting in disruption of lipid metabolism, respiration and production of ATP. Mancozeb is a foliar fungicide with protective action.
Propargite is a sulfite ester acaricide described in US 3463859 (Chemtura) and can be used to control phytophagous mites {particularly motile stages) on a variety of crops including vines, fruit trees (e.g. top fruit, stone fruit and citrus fruit), hops, nuts, tomatoes, vegetables, ornamentals, cotton, maize, peanuts and sorghum.
PROPARGITE

IUPAC NAME: 2-(4-tert-butylphenoxy)cycIohexyI prop-2-ynyl sulfite CAS Number: (2312-35-8) MOLECULAR WEIGHT: 350.5

MOLECULAR FORMULA: C19H26O4S
Water solubility: In water 0.215 mg/1 (25 °C). Fully miscible with hexane, toluene,
dichloromethane, methanol and acetone.
Form: Brownish-yellow, oily viscous liquid (tech.)
Uses: Propargite has fair action of killing mites and can kill most kinds of mites,
especially mites harmful to crops like cotton, vegetables, apple, orange, tea, flowers,
etc. It is little harmful to environment. Control of phytophagous mites (particularly
motile stages) on a variety of crops including vines, fruit trees (e.g. top fruit, stone
fruit and citrus fruit), hops, nuts, tomatoes, vegetables, ornamentals, cotton, maize,
peanuts and sorghum. Used at rates from 0.75-1.8 kg/ha on row crops, and foliar
sprays of 0.85-3.0 kg/ha on perennial fruit and nut crops.
Mode of Action : Propargite is a broad-spectrum, to the adult mites and mites have special effects, if can be used to prevent cotton, vegetables, apple, orange, tea, flowers and other crops various harmful acarids, security for most natural enemy.
Propargite is non-systemic acaricide with predominantly contact action; some additional action by inhalation.
Although there are numerous chemical compounds fungicides and acaricides which aid in preventing diseases and mites of plants, each of these have practical deficiencies, which restrict their use. The inability of any one fungicide to control all of the potentially serious fungi / phytophagous mites on one-host makes it necessary to use combinations or mixtures. Therefore, the scientific community concentrated on developing an effective combination to overcome the drawback associated with the use of single biocide. The prior art references that deal with combinations of biocides are as follows.

Runze Han et al. CN101496523 (2008) discloses a germicide composition, which is wettable powder containing polyoxin and Mancozeb. The composition comprises 0.1 to 10 % of polyoxin, 5 to 90 % of Mancozeb, and the balance being additives.
Jingmin Zheng et al.CNl01697728 (2009) discloses an acaricide compound containing biphenyl hydrazine ester. The weight precentage ratio of the first active ingredient: biphenyl hydrazine ester and the second active ingredient: abamectin, pyridaben or Propargite of the compound is (5 to 40): 1, (0.5 to 8):1, (0.125 to 0.5): 1. The acaricide compound can be prepared using water dispersed particle agent and suspending agent in the form of wettable powder, missible oil or aqueous emulsion. The compound can be applied to the citrus red mite, European red mite and mite on cotton plants.
Zhongyi Liu At AI. CN101653120 (2009) discloses a mite-killing composition comprising fenazaquin and Propargite, wherein the ratio of the fenazaquin and the Propargite in parts by mass is 10:1-1:75.
Shaowu Zhang At Al. CN101416627 (2008) discloses an agricultural composition for killing acarus, specifically a compound composition containing Propargite and fenisobromolate. The disclosure is characterized in that the active components of Propargite and fenisobromolate in terms of percentage by weight is 1-60 : 0.1:50, and the composition can be prepared into aqueous emulsion by adding proper amount of additive agents or carriers for preventing and controlling red spider lived in orange.
Shaowu Zhang et al CN101406182 (2008) discloses a mite-killing composition containing Propargite and fenisobromolate. The formulation is missible oil or a microemulsion, wherein the content of the Propargite is between 10 and 90 %, and the content of the fenisobromolate is between 20 and 80 %.
Jingmin Zheng et al.CNl 01297646 (2008) discloses an acaricide composite containing spirodiclofen and application thereof. The composition comprises (A) a

first active ingredient: spirodiclofen and (B) a second active ingredient: Propargite wherein the weight ratio of the first active ingredient and the second active ingredients is 0.01 to 10:1.
Jingmin Zheng et al. CN101297647 (2008) discloses an acaricide composite containing chlorfenapyr and application thereof. The composition comprising (A) a first active ingredient: chlorfenapyr and (B) a second active ingredients: Propargite wherein the weight ratio of the first active ingredient and the second active ingredients is 0.02 to 10:1.
Linxi Bian et al. CN101584318 (2008) discloses an insect and acarid killer which is a binary composition compounded by taking spiromesifen, fenpyroximate and clofentezine or Propargite as essential components, wherein the weight ratio of the spiromesifen to the fenpyroximate or the spiromesifen to the clofentezine or the spiromesifen to the Propargite is 60:1 to 1:60.
Yaolun Ge et al. CN101138340 (2006) discloses an acaricide combination which contains fenbutatin oxide and Propargite. The combination is in the form of emulsion, missible oil and suspending emulsion. The weight proportion of the fenbutatin oxide and Propargite is 1-40 to 1-60.
Wang Zhengquan et al. CN1449665 (2003) discloses an acaricide composition comprising a mixture of fenpropathrin and Propargite in a weight ratio of 1:0.05- 1:5, and the weight percentage content of its effective component is 1-80%.
The reported composition are associated with several drawbacks such as they have low activity, they may cause harm to crops, they does not meet safety standards of agricultural chemical preparation and are expensive to manufacture.
In view of the above, there is envisaged in accordance with the present disclosure an environmentally safe synergistic biocidal composition which is effective at lower

dose. Further, the inventors of the present disclosure also envisaged a simple and economic process to prepare a biocidal composition.
OBJECTS
Some of the objects of the present disclosure, which at least one embodiment herein satisfies, are as follows:
It is an object of the present disclosure to provide a synergistic biocidal composition to control potentially serious fungi and phytophagous mites.
It is another object of the present disclosure to provide a process for preparing a synergistic biocidal composition which is simple and economic.
It is yet another object of the present disclosure to provide a kit comprising a synergistic biocidal composition which is capable of controlling fungi and phytophagous mites.
Other objects and advantages of the present disclosure will be more apparent from the following description, which is not intended to limit the scope of the present
disclosure.
In accordance with one aspect of the present disclosure there is provided a synergistic biocidal composition comprising:
a. Propargite in an amount ranging between 2 % and 35 % with respect to the
total mass of the composition; and
b. Mancozeb in an amount ranging between 30 % and 80 % with respect to
the total mass of the composition,
wherein the proportion of Propargite to Mancozeb ranges between 1:1 and 1:40

The synergistic biocidal composition of the present disclosure further comprises at least one excipient selected from the group consisting of surfactant, stabilizer, filler, solubilizer, binding agent and defoaming agent, wherein the total amount of the excipient ranges between 0.5 % and 40 % with respect to the total mass of the composition.
Typically, the surfactant is at least one selected from the group consisting of sodium ligno sulphonate, modified sodium lignosulfonate, sulfonated naphthalene condensate sodium salt, sodium alkyl naphthalene sulphonate, blend of Sodium alkyl naphthalene sulphonate and anionic surfactant, sulfonated aromatic polymer sodium salt, sodium lauryl sulphate, blend of anionic surfactants belonging to class of naphthalene sulphonate, sodium salt of dibutyl naphthalene sulphonate, blend of alcohol alkoxylate and fatty alcohol ethoxylates and sodium salt of polycarboxylic acid, in an amount ranging between 5 % and 15 % with respect to the total mass of the composition.
Typically, the stabilizer is at least one selected from the group consisting of hexamethylene tetra amine and sodium meta bisulphite, in an amount ranging between 0.5 % and 5% with respect to the total mass of the composition.
Typically, the filler is at least one selected from the group consisting of silica and kaolin clay, in an amount ranging between 1 % and 30% with respect to the total mass of the composition.
Typically, the solubilizer is at least one selected from the group consisting of calcium salt of alkyl benzene sulphonates, castor oil ethoxylates, sulfonated natural oils and fats, sulfonate esters and alkyl phenol ethoxylates, in an amount ranging between 1 % and 10% with respect to total mass of the composition.

Typically, the binding agent is at least one selected from the group consisting of dextrose and poly vinyl alcohol, in an amount ranging between 0.5% and 5% with respect to the total mass of the composition.
Typically, the defoaming agent is poly dimethyl siloxane in an amount ranging between 0.5 and 5% with respect to the total mass of the composition.
Typically, the composition is in the form selected from the group consisting of aerial sprays, directly sprayable solutions, wettable powders, suspensions, dispersions, emulsions, oil dispersions, pastes and wettable granules.
In one embodiment of the present disclosure the synergistic biocidal composition of the present disclosure is in a wettable powder form; said composition comprising:
a. Propargite in an amount ranging between 10 % and 25 % with respect to the
total mass of the composition,
b. Mancozeb in an amount ranging between 30 % and 45 % with respect to
the total mass of the composition,
c. at least one surfactant selected from the group consisting of sodium ligno
sulphonate, modified sodium lignosulfonate, sulfonated naphthalene
condensate sodium salt, sodium alkyl naphthalene sulphonate, blend of
Sodium alkyl naphthalene sulphonate and anionic surfactant, sulfonated
aromatic polymer sodium salt, sodium lauryl sulphate, blend of anionic
surfactants belonging to class of naphthalene sulphonate, sodium salt of
dibutyl naphthalene sulphonate, blend of alcohol alkoxylate and fatty
alcohol ethoxylates, in an amount ranging between 5 % and 15 % with
respect to the total mass of the composition
d. at least one filler selected from the group consisting of silica and kaolin
clay, in an amount ranging between 1 % and 30 % with respect to the total
mass of the composition,

e. at least one solubilizer selected from the group consisting of calcium salt of
alkyl benzene sulphonates, castor oil ethoxylates, sulfonated natural oils
and fats, sulfonate esters, alkyl phenol ethoxylates, in an amount ranging
between 1 % and 5 % with respect to the total mass of the composition, and
f. optionally, at least one stabilizer selected from the group consisting of
hexamethylene tetra amine and sodium meta bisulphite, in an amount
ranging between 0.5 % and 5 % with respect to the total mass of the
composition.
In another embodiment of the present disclosure the synergistic biocidal composition of the present disclosure is in a wettable granule form; said composition comprising:
a. Propargite in an amount ranging between 10 % and 25% with respect to the
total mass of the composition,
b. Mancozeb in an amount ranging between 30 % and 45 % with respect to
the total mass of the composition,
c. at least one surfactant selected from the group consisting of sodium ligno
sulphonate, modified sodium lignosulfonate, sulfonated aromatic polymer
sodium salt, sodium salt of polycarboxylic acid, sodium alkyl naphthalene
sulphonate, sodium salt of dibutyl naphthalene sulphonate, sodium alkyl
naphthalene sulphonate, in an amount ranging between 5 % and 15 % with
respect to the total mass of the composition,
d. at least one filler selected from the group consisting of silica and kaolin
clay, in an amount ranging between 1 % and 30 % with respect to the total
mass of the composition,
e. at least one stabilizer selected from the group consisting of hexamethylene
tetra amine and sodium meta bisulphite, in an amount ranging between 0.5
% and 5 % with respect to the total mass of the composition,
f. at least one binding agent selected from the group consisting of dextrose,
poly vinyl alcohol, in an amount ranging between 0.5 % and 5 % with
respect to the total mass of the composition,

g. dimethyl siloxane as a defoaming agent, in an amount ranging between 0.5 % and 5 % with respect to the total mass of the composition, and
h. water in an amount ranging between 40% and 55 % with respect to the total mass of the composition.
In accordance with another aspect of the present disclosure there is provided a kit comprising;
i. Propargite as a component A;
ii. Mancozeb as a component B; and
iii. at least one packaging material for packaging the components (A) and (B),
The kit of the present disclosure further comprises at least one excipient selected from the group consisting of surfactant, stabilizer, filler, solubilizer, binding agent and defoming agent.
In accordance with the present disclosure a process for preparing a synergistic biocidal composition in the form selected from the group consisting of aerial sprays, directly sprayable solutions, wettable powders, suspensions, dispersions, emulsions, oil dispersions, pastes and wettable granules; said process comprising at least one step selected from the group consisting of mixing, pulverizing, blending, wet milling, spray drying and extruding.
In accordance with one of the embodiments of the present disclosure there is provided a process for preparing a synergistic biocidal composition in the form of wettable powder, said process comprising the following steps:
a. mixing Propargite with at least one excipient selected from the group
consisting of surfactant, solubilizer, filler and stabilizer to obtain a
mixture;

b. pulverizing the mixture in a pulverizer to obtain a pulverized mixture
containing 2 to 7 micron sized particles; and
c. blending the pulverized mixture with Mancozeb or Mancozeb mixture
containing at least one excipient selected from the group consisting of
surfactant, solubilizer, filler and stabilizer to obtain a synergistic
biocidal composition in a wettable powder form.
In accordance with another embodiment of the present disclosure there is provided a process for preparing a synergistic biocidal composition in the form of wettable granules, said process comprising the following steps:
a. mixing Mancozeb, at least one excipient selected from the group
consisting of surfactant, filler, binding agent, defoaming agent and
stabilizer, and water to obtain a slurry having total solids in the range
of 40 to 55%;
b. wet milling the slurry to obtain wet milled slurry containing 2 to 7
micron sized particles;
c. blending the wet milled slurry with Propargite or Propargite mixture
containing at least one excipient selected from the group consisting of
surfactant, stabilizer, filler, binding agent and defoaming agent to
obtain slurry having total solid content in the range of 45 % to 60% and
d. spray drying the slurry to obtain a synergistic biocidal composition in
a wettable granules form having particle size in the range of 100 to
1000 micron.
In accordance with yet another embodiment of the present disclosure there is provided a process for preparing a synergistic biocidal composition in the form of wettable granules, said process comprising the following steps:
a. mixing Propargite with at least one excipient selected from the group
consisting of surfactant, stabilizer, filler, binding agent and defoaming
agent to obtain a mixture;

b. pulverizing the mixture in a pulverizer to obtain a pulverized mixture
containing 2 to 7 micron sized particles;
c. blending the pulverized mixture with Mancozeb and water;
d. extruding the mixture through an extruder to obtain a synergistic
biocidal composition in a wettable granules form having particle size in
the range of 100 to 2000 microns.
DETAILED DESCRIPTION:
In accordance with one aspect of the present disclosure there is provided a synergistic biocidal composition comprising a fungicide and an acaricide for effective controlling of fungi and mites. The acaricide in the composition of the present disclosure is Propargite whereas the fungicide is Mancozeb. The amount of Propargite in the composition varies between 2 % and 35 % with respect to the total mass of the composition whereas the amount of Mancozeb ranges between 30 % and 80 % with respect to the total mass of the composition. The inventors of the present invention found that the biocidal composition of the present disclosure is effective when the proportion of Propargite to Mancozeb ranges between 1:1 and 1:40 .The biocidal composition for the purpose of effective application may further comprise at least one excipient selected from the group consisting of surfactant, stabilizer, filler, solubilizer, binding agent and defoming agent. The use of the excipient in the composition facilitates the application of the biocidal composition. The quantity of the excipient in the biocidal composition optimized by the inventors of the present disclosure ranges between 0.5 % and 40 % with respect to the total mass of the composition.
Surfactants lower the surface tension of a liquid, the interfacial tension between two liquids, or liquid and solid. The requisite level of reduction in the surface or interfacial tension of the composition of the present disclosure is achieved by using appropriate surfactant in an appropriate amount in the composition. The surfactant identified for use in the biocidal composition of the present disclosure includes sodium ligno

sulphonate, modified sodium lignosulfonate, sulfonated naphthalene condensate sodium salt, sodium alkyl naphthalene sulphonate, blend of Sodium alkyl naphthalene sulphonate and anionic surfactant, sulfonated aromatic polymer sodium salt, sodium lauryl sulphate, blend of anionic surfactants belonging to class of naphthalene sulphonate, sodium salt of dibutyl naphthalene sulphonate, blend of alcohol alkoxylate and fatty alcohol ethoxylates, sodium salt of polycarboxylic acid and combinations thereof. The amount of the surfactant in the biocidal composition that provides required level of reduction in surface tension or interfacial tension ranges between 5 % and 15 % with respect to the total mass of the composition. Surfactants used in the biocidal composition of the present disclosure may also serve a function of detergent, wetting agent, emulsifier, foaming agent and dispersant/suspending agent.
These surfactants in the composition of the present disclosure increase the spreading and penetrating properties of a liquid by lowering tendency of the liquid molecules to adhere to each other at the surface. It stretches the bonds and decreases the tendency of molecules to bond together, which allows the composition to spread more easily across any solid surface.
The stabilizer used in the composition of the present disclosure is at least one selected from the group consisting of hexamethylene tetra amine and sodium meta bisulphite. The amount of the stabilizer used in the present composition is such that it effectively stabilizes the composition for longer time period. The amount of the stabilizer ranging between 0.5 % and 5% with respect to the total mass of the composition can be utilized to stabilize the composition.
The filler used in the biocidal composition is at least one selected from the group consisting of silica, kaolin clay in an amount ranging between 1 % and 30% with respect to the total mass of the composition.

The biocidal composition comprises the solubilizer selected from the group consisting of calcium salt of alkyl benzene sulphonates, castor oil ethoxylates, sulfonated natural oils and fats, sulfonate esters, alkyl phenol ethoxylates and combinations thereof ranges between 1 % and 10% with respect to total mass of the composition.
A binding agent is added to the biocidal composition to cause adhesion of powder particles granulations or to hold or draw components of the composition together to form a cohesive whole. The binding agent is selected from the group consisting of dextrose, poly vinyl alcohol and combinations thereof and its amount in the composition ranges between 0.5 % and 5% with respect to the total mass of the composition.
The amount of defoaming agent ranges between 0.5 % and 5% with respect to the total mass of the composition. Poly dimethyl siloxane is used as a defoaming agent. The purpose of adding defoaming agent in the composition is to reduce and hinder the formation of foam during the process of preparing the biocidal composition of the present disclosure.
In accordance with the present disclosure the synergistic biocidal composition of the present disclosure is in the form selected from the group consisting of aerial sprays, directly sprayable solutions, wettable powders, suspensions, dispersions, emulsions, oil dispersions, pastes and wettable granules.
In one of the embodiments of the present disclosure the synergistic biocidal composition is in a wettable powder form. The wettable powder form of the biocidal composition comprises:
a. Propargite in an amount ranging between 10 % and 25 % with respect to the
total mass of the composition,
b. Mancozeb in an amount ranging between 30 % and 45 % with respect to
the total mass of the composition,

c. at least one surfactant selected from the group consisting of sodium ligno
sulphonate, modified sodium lignosulfonate, sulfonated naphthalene
condensate sodium salt, sodium alkyl naphthalene sulphonate, blend of
Sodium alkyl naphthalene sulphonate and anionic surfactant, sulfonated
aromatic polymer sodium salt, sodium lauryl sulphate, blend of .anionic
surfactants belonging to class of naphthalene sulphonate, sodium salt of
dibutyl naphthalene sulphonate, blend of alcohol alkoxylate and fatty
alcohol ethoxylates, in an amount ranging between 5 % and 15 % with
respect to the total mass of the composition
d. at least one filler selected from the group consisting of silica and kaolin
clay, in an amount ranging between 1 % and 30 % with respect to the total
mass of the composition,
e. at least one solubilizer selected from the group consisting of calcium salt of
alkyl benzene sulfonates and castor oil ethoxylates, in an amount ranging
between 1 % and 5 % with respect to the total mass of the composition, and
f. optionally, at least one stabilizer selected from the group consisting of
hexamethylene tetra amine and sodium meta bisulphite, in an amount
ranging between 0.5 % and 5 % with respect to the total mass of the
composition.
In another embodiment of the present disclosure the synergistic biocidal composition is in a wettable granule form. The wettable granule form of the biocidal composition comprises:
a. Propargite in an amount ranging between 10 % and 25 % with respect to the
total mass of the composition,
b. Mancozeb in an amount ranging between 30 % and 45 % with respect to
the total mass of the composition,
c. at least one surfactant selected from the group consisting of sodium ligno
sulphonate, modified sodium lignosulfonate, sulfonated aromatic polymer
sodium salt, sodium salt of polycarboxylic acid, sodium alkyl naphthalene

sulphonate, sodium salt of dibutyl naphthalene sulphonate, sodium alkyl naphthalene sulphonate, in an amount ranging between 5 % and 15 % with respect to the total mass of the composition,
d. at least one filler selected from the group consisting of silica and kaolin
clay, in an amount ranging between 1 % and 30 % with respect to the total
mass of the composition,
e. at least one stabilizer selected from the group consisting of hexamethylene
terra amine and sodium meta bisulphite, in an amount ranging between 0.5
% and 5 % with respect to the total mass of the composition,
f. at least one binding agent selected from the group consisting of dextrose
and poly vinyl alcohol, in an amount ranging between 0.5 % and 5% with
respect to the total mass of the composition,
g. poly dimethyl siloxane as a defoaming agent, in an amount ranging between
0.5 % and 5 % with respect to the total mass of the composition, and
h. water in an amount ranging between 40 % and 55 % with respect to the total mass of the composition.
In accordance with another aspect of the present disclosure there is provided a kit for controlling pests and fungus. The kit comprises Propargite as a component A; Mancozeb as a component B; and at least one packaging material for packaging the components (A) and (B). The kit may further comprises at least one excipient selected from the group consisting of surfactant, stabilizer, filler, solubilizer, binding agent and defoming agent.
In accordance with yet another aspect of the present disclosure there is provided a process for preparing a synergistic biocidal composition in a form selected from the group consisting of aerial sprays, directly sprayable solutions, wettable powders, suspensions, dispersions, emulsions, oil dispersions, pastes and wettable granules. The synergistic biocidal composition of the present disclosure is prepared by using at least one step selected from the group consisting of mixing, pulverizing, blending, wet milling, spray drying and extruding.

In one embodiment of the present disclosure there is provided a process for preparing the synergistic biocidal composition in a wettable powder form. In the first step, Propargite is mixed with at least one excipient selected from the group consisting of surfactant, solubilizer, filler and stabilizer to obtain a mixture. The mixture is pulverized in a pulverizer to obtain a pulverized mixture containing 2 to 7 micron sized particles. The pulverized mixture is then blended with either Mancozeb or Mancozeb mixture containing at least one excipient selected from the group consisting of surfactant, solubilizer, filler and stabilizer to obtain a synergistic biocidal composition in a wettable powder form.
In another embodiment of the present disclosure there is provided a process for preparing a synergistic biocidal composition in a wettable granules form. To prepare a wettable granule form, Mancozeb is first mixed with at least one excipient selected from the group consisting of surfactant, filler, binding agent, defoaming agent and stabilizer in the presence of water to obtain slurry having total solids in the range of 40 to 55%. Secondly, the slurry is wet milled to obtain wet milled slurry containing 2 to 7 micron sized particles. Finally, the wet milled slurry is blended with either Propargite or Propargite mixture containing at least one excipient selected from the group consisting of surfactant, stabilizer, filler, binding agent and defoaming agent to obtain slurry having total solid content in the range of 45 to 60% followed by spray drying the slurry to obtain a synergistic biocidal composition in a wettable granules form. The particle size of the biocidal composition obtained by this process ranges between 100 and 1000 micron.
The present disclosure also provides an alternate process for preparing a synergistic biocidal composition in a wettable granules form. In this alternate process, Propargite is mixed with at least one excipient selected from the group consisting of surfactant, stabilizer, filler, binding agent and defoaming agent to obtain a mixture. The mixture is then pulverized in a pulverizer to obtain a pulverized mixture containing 2 to 7 micron sized particles. The pulverized mixture is then blended with Mancozeb in the presence of water which is then extruded in an extruder to obtain a synergistic biocidal

composition in a wettable granule form. The particle size of the biocidal composition obtained by this process ranges between 100 and 2000 micron.
The present disclosure is further described in light of the following examples which are set forth for illustration purpose only and not to be construed for limiting the scope of the disclosure.
I. Preparation of wettable powder form of the biocidal composition of the present disclosure.
Example 1:
22.8 gm of Propargite is mixed with 7 gm of silica, 2 gm of kaolin clay, 4 gm of calcium salt of alkyl benzene sulphonate, 7 gm of sodium lignosulphonate and 3 gm of sodium alkyl naphthalene sulphonate and 1.5 gm of Hexamethylenetetramine to obtain a mixture. The mixture is pulverized in a pulverizer to obtain a pulverized mixture containing 2 to 7 micron sized particles. The pulverized mixture is then blended with 54.7 gm of Mancozeb to obtain a biocidal composition in a wettable powder form.
Example 2:
22.8 gm of Propargite is mixed with 11 gm of silica, 1 gm of sodium metabisulphite, 2.5 gm ofcastor oil ethoxylates, 5 gm of sodium ligno sulphonate, 2 gm of sodium alkyl naphthalene sulphonate and 4 gm of sulfonated aromatic polymer sodium salt to obtain a mixture. The mixture is pulverized in a pulverizer to obtain a pulverized mixture containing 2 to 7 micron sized particles. The pulverized mixture is then blended with 54.7 gm of Mancozeb, to obtain a biocidal composition in a wettable powder form.
Example 3:
22.5 gm of Propargite is mixed with 12 gm of silica, 2 gm of kaolin clay, 2 gm of castor oil ethoxylates, 5 gm of sulfonated aromatic polymer sodium salt and1.5 gm of

blend of alcohol alkoxylated and fatty alcohol ethoxylates to obtain a mixture. The mixture is pulverized in a pulverizer to obtain a pulverized mixture containing 2 to 7 micron sized particles. The pulverized mixture is then blended with 55.0 gm of Mancozeb to obtain a biocidal composition in a wettable powder form.
Example 4:
18 gm of Propargite is mixed with 12 gm of silica, 5 gm of kaolin clay, 3 gm of castor oil ethoxylates, 7 gm of sodium lignosulphonate, 3.5 gm of blend of anionic surfactants belonging to class of naphthalene sulfonate, 2 gm sulfonated aromatic polymer sodium salt to obtain a mixture. The mixture is pulverized in a pulverizer to obtain a pulverized mixture containing 2 to 7 micron sized particles. The pulverized mixture is then blended with 49.50 gm of Mancozeb to obtain a biocidal composition in a wettable powder form.
Example 5:
18 gm of Propargite is mixed with 10 gm of silica, 8 gm of kaolin clay, 4 gm of castor oil ethoxylates, 7 gm of sodium lignosulphonate, 2.5 gm of blend of anionic surfactants belonging to class of naphthalene sulfonate, 1 gm of sodium alkyl naphthalene sulphonate to obtain a mixture. The mixture is pulverized in a pulverizer to obtain a pulverized mixture containing 2 to 7 micron sized particles. The pulverized mixture is then blended with 49.50 gm of Mancozeb to obtain a biocidal composition in a wettable powder form.
Example 6:
18 gm of Propargite is mixed with 13 gm of silica, 8 gm of kaolin clay, 4 gm of castor oil ethoxylates, 5 gm of sodium lignosulphonate, 1.5 gm of sulfonated naphthalene condensate, sodium salt, 1 gm of blend of alcohol alkoxylated and fatty alcohol ethoxylates to obtain a mixture. The mixture is pulverized in a pulverizer to obtain a pulverized mixture containing 2 to 7 micron sized particles. The pulverized mixture is then blended with 49.50 gm of Mancozeb to obtain a biocidal composition in a wettable powder form.

Example 7:
13.8 gm of Propargite is mixed with 10 gm of silica, 15.2 gm of kaolin clay, 3 gm of castor oil ethoxylates, 5 gm of sulfonated naphthalene condensate, sodium salt, 4 gm of blend of anionic surfactants belonging to class of naphthalene sulfonate, 2-gm of sodium salt of dibutyl naphthalene sulphonate to obtain a mixture. The mixture is pulverized in a pulverizer to obtain a pulverized mixture containing 2 to 7 micron sized particles. The pulverized mixture is then blended with 47 gm of Mancozeb to obtain a biocidal composition in a wettable powder form.
Example 8:
13.8 gm of Propargite is mixed with 10 gm of silica, 15.2 gm of kaolin clay, 3 gm of castor oil ethoxylates, 6 gm of sodium Hgnosulphonate, 3 gm of sulfonated aromatic polymer sodium salt and 2.0 gm of blend of alcohol alkoxylated and fatty alcohol ethoxylates to obtain a mixture. The mixture is pulverized in a pulverizer to obtain a pulverized mixture containing 2 to 7 micron sized particles. The pulverized mixture is then blended with 47.0 gm of Mancozeb to obtain a biocidal composition in a wettable powder form.
Example 9:
13.8 gm of Propargite is mixed with 10 gm of silica, 15.2 gm of kaolin clay, 3 gm of castor oil ethoxylates, 6 gm of sodium Hgnosulphonate, 3.5 gm of sulfonated aromatic polymer sodium salt and 1.5 gm of sodium alkyl naphthalene sulphonate to obtain a mixture. The mixture is pulverized in a pulverizer to obtain a pulverized mixture containing 2 to 7 micron sized particles. The pulverized mixture is then blended with 47.0 gm of Mancozeb to obtain a biocidal composition in a wettable powder form. The results of physical parameters for the powder prepared in examples 1 to 9 at zero day and 14 days (on ageing at 54°C±2C.) are provided in Table 1 herein below. The powder obtained in all the above examples was yellow in appearance.

Table 1:

Sr.
No Parameter
5 EX.1 EX.2 EX.3 EX.4 EX. 5 EX.6 EX. 7 EX. 8 EX.9
Od 14
d* Od 14
d* Od 14 d* Od 14
d* Od 14
d* Od 14 d* Od 14 d* Od 14 d* 0d 14 d*
1 Active
content
Mancozeb
(WAV) 46.3 46.3 45.4 45.4 45.9 45. 9 41.8 41 41.7 4 41. 67 41.6 41. 2 39 38. 4 39.6 39.46 39.75 39.68
2 Active content Propargite (WAV) 20.5 20.5 20.56 205 6 20.3 6 20. 36 16.4 15
5 16 3 8 16.
08 16.2 5 15, 9 12.8 12. 3 12.4 5 12.38 12.58 12.5
3 Suspensibilit y(%W/W) 56 54 77 76 69 65 63 61 75 74 69 63 65 48 62 52 79 78
4 Wetting out time (Sec.) 45. 45 23. 23 79. 79 47 48 29 33 27 27 69 75 65 68 41 39
5 pH (1% solution) 6.8 6.8 6.8 6.8 7.1 7.1 7.2 7,1 6.8 6.9 6.9 6.9 6.5 6.8 6.8 7 7.1 7.2
14d* : at 54°C±2°C for 14-days
II. Preparation of wettable granule form of the biocidal composition of the present disclosure.
EXAMPLE 10:
190gm of Mancozeb was added slowly with continuous mixing to 200gm of water in a repulp tank fitted with a homogeniser from Remi Motors Ltd. To the mixture was added 8gm of Sodium alkyl naphthalene sulphonate, 3gm of Hexamethylene terra amine and 0.5gm of poly Dimethyl Siloxane Powder. The homogeniser/Agitator was operated at 3000rpm for 25minutes to obtain slurry. The slurry was pumped by using double diaphragm pump to a wet mill (Dyno mill) at a flow rate of 50ml/min. Particle size was analysed by Malvern particle size analyser. The milling was continued till the desired particle size in the range of 1.5 to 7 micron was obtained. The finely milled slurry was pumped to the mix blend tank where Propargite (53gm) was mixed with Silica Powder 30gm, Kaolin Clay 55.5gm, Modified Sodium Ligno Sulphonate 25gm, Sulphonated aromatic polymer sodium salt 15gm and Dextrose 5gm. This mixture in the blend tank was operated at 2000rpm for 30 minutes to obtain a homogeneous substance. Additional water was added to obtain solid particulate substance of the slurry between 50 to 65% (Percentage of particulate matter was checked by loss on drying method). Slurry was then spray dried by a Spray Dryer. The

material obtained was sieved through the sieve shaker to obtain granule size in the range of 100-600microns.
EXAMPLE 11:
190gm of Mancozeb was added slowly with continuous mixing to 200gm of water in a repulp tank fitted with a homogeniser from Remi Motors Ltd. To the mixture was added 25gm of Sodium salt of alkyl naphthalene sulphonate, 3gm of Hexamethylene tetra amine ,0.5gm of poly Dimethyl Siloxane Powder. The homogeniser/Agitator was operated at 3000rpm for 25minutes to obtain slurry. The slurry was pumped by using double diaphragm pump to a wet mill (Dyno mill) at a flow rate of 50ml/min. Particle size was analysed by Malvern particle size analyser. The milling was continued till the desired particle size in the range of 1.5 to 7 micron was obtained. The finely milled slurry was pumped to the mix blend tank where Propargite (53gm) was mixed with Silica Powder 30gm, Kaolin Clay 52.5gm, Sodium Ligno Sulphonate 20gm, Sulphonated aromatic polymer sodium salt 10gm and Poly Vinyl Alcohol 1gm. This mixture in the blend tank was operated at 2000rpm for 30 minutes to obtain a homogeneous substance. Additional water was added to obtain solid particulate substance of the slurry between 50 to 65% (Percentage of particulate matter was checked by loss on drying method). Slurry was then spray dried by a Spray Dryer. The material obtained was sieved through the sieve shaker to obtain granule size in the range of 100-600microns.
EXAMPLE 12:
192gm of Mancozeb was added slowly with continuous mixing to 200 gm of water in a repulp tank fitted with a homogeniser from Remi Motors Ltd. To the mixture was added 15 gm of Sodium salt of alkyl naphthalene sulphonate, 3gm of Hexamethylene tetra amine, 0.5gm of poly Dimethyl Siloxane Powder, The homogeniser/Agitator was operated at 3000rpm for 25minutes to obtain slurry. The slurry was pumped by using double diaphragm pump to a wet mill (Dyno mill) at a flow rate of 50ml/min. Particle size was analysed by Malvern particle size analyser. The milling was continued till the desired particle size in the range of 1.5 to 7 micron was obtained. The finely milled

slurry was pumped to the mix blend tank where Propargite (55gm) was mixed with Silica Powder 30gm, Kaolin Clay 66.5gm, Sodium Ligno Sulphonate 30gm, Sodium salt of Polycarboxylic Acid 10gm and Poly Vinyl Alcohol 1gm.
This mixture in the blend tank was operated at 2000rpm for 30 minutes to obtain a homogeneous substance. Additional water was added to obtain solid particulate substance of the slurry between 50 to 65% (Percentage of particulate matter was checked by loss on drying method) The slurry was then spray dried by a Spray Dryer. The material obtained was sieved through the sieve shaker to obtain granule size in the range of 100-600microns.
EXAMPLE 13:
182gm of Mancozeb was added slowly with continuous mixing to 200gm water in a repulp tank fitted with a homogeniser from Remi Motors Ltd. To the mixture was added 10gm of Sodium salt of dibutyl naphthalene sulphonate, 3gm of Hexamethylene tetra amine ,0.5 gm of poly Dimethyl Siloxane Powder. The homogeniser/Agitator was operated at 3000rpm for 25minutes to obtain slurry. The slurry was pumped by using double diaphragm pump to a wet mill (Dyno mill) at a flow rate of 50ml/min. Particle size was analysed by Malvern particle size analyser. The milling was continued till the desired particle size in the range of 1.5 to 7 micron was obtained. The finely milled slurry was pumped to the mix blend tank where Propargite 55gm was mixed with Silica Powder 30gm, Kaolin Clay 86.5gm, Modified Sodium Ligno Sulphonate 35gm,and Poly Vinyl Alcohol lgm.
This mixture in the blend tank was operated at 2000rpm for 30min. to obtain a homogeneous substance. Additional water was added to obtain solid particulate substance of the slurry between 50 to 65% (Percentage of particulate matter was checked by loss on drying method) The slurry was then spray dried by a Spray Dryer. The material obtained was sieved through the sieve shaker to obtain granule size in the range of 100-600microns.

The results of physical parameters for the granules prepared in examples 10 to 13 at zero day and 14 days are provided in Table 2 herein below. The powder obtained in all the above examples was yellowish brown in appearance.
Table 2:

Sr. No. Parameters EX.10 EX11 EX.12 EX.13
Od 14 d Od 14 d Od 14 d Od 14 d
1 Active content Mancozeb (WAV) 41.5 41.4 41.7 40 40.65 39 38.2 38.1
2 Active content Propargite
(W/W) 12.38 12.3 12.29 11.5 12.3 11.2 12.56 12.39
3 Suspensibilit y (%W/W) 78 75 64 54 59 52 81 78
4 Wetting out time (Sec.) 16 15 20 25 23 29 8 11
5 pH (1% solution) 7.2 7.2 7.4 7.3 7.2 7 7.1 7.1
III. Experimental trials of the composition of the present disclosure to study efficacy and phototoxicity on Chilli-334
Design : Randomized Block Design (RBD)
Spacing : 70 x 30 cm
Plot size : 5 m X 4 m (20 sq. m)
Time of spray applications : 1st on 40 days after transplanting (DAT) and
2nd on 55 days after transplanting (15th day after 1st application)
Pest Observed : Fungal Leaf spot (Cercospora &
Colletotrichum sp.) and Yellow Mites (Polyphagotarsonemus latus)

TREATMENT DETAILS: Efficacy study:

No. Treatment Dose (gm of active ingredient/ha) Dose (gm/ha)
Tl Mancozeb 45% + Propargite 20% WP 1350+600 3000
T2 Mancozeb 40% + Propargite 15% WP 1200+450 3000
T3 Mancozeb 38 % + Propargite 12% WP 1140+360 3000
T4 Mancozeb 75% WP 1500 2000
T5 Propargite 57% EC 850 1500
T6 Control — —
Phytotoxicity study:

No. Treatment Dose (gm of active ingredient/ha) DOSE (g /ha)
Tl Mancozeb 45% + Propargite 20% WP 2700+1200 6000
T2 Mancozeb 40% + Propargite 15% WP 2400+900 6000
T3 Mancozeb 38 % +Propargite 12% WP 2280+720 6000
T4 Control Water spray
METHODOLOGY
Efficacy:
Twenty randomly selected plants per plot were tagged and scored for the incidence of leaf spot and mite population on the leaves. The treatments were applied and incidences of Leaf spot (Cercospora and Colletotrichum sp.) and Yellow mites were

recorded at a time interval of 7th, 15th and 21st day after second application. Chilli leaf spot was scored in plots using disease scoring scale as mentioned below. The results are provided in Table 3.
Disease scoring scale for leaf spot (Cercospora and Colletotrichum sp.):

0 No symptoms
1 <5% of the leaf area showing spots
3 6-20% leaf area showing spots on the leaves
5 21-50% leaf area is showing big spots on the leaves
7 51-70% leaf area is covered with the spots and drying of leaves
9 70 % leaves tend to drying; dropping of lower leaves
A disease infection was then calculated using the following formula;

The fruit yield of each net plot was recorded at every picking time to final harvest.
b) Phytotoxicity:
For phytotoxicity double dose of test chemicals were taken and all other experimental conditions were kept constant. Observations on leaf injury, wilting, vein clearing, necrosis, epinasty and hyponasty were recorded on ten randomly selected Chilli plants from each plot at 0, 3, 7 and 10 days after application (DAA). The level of phytotoxicity was estimated by visual assessment on below mentioned scale of 0-10. The results are provided in Table 4.

% injury
Rating % injury Rating
0 No phytotoxicity 51-60 6
1-10 1 61-70 7
11-20 2 71-80 8
21-30 3 81-90 9
31-40 4 91-100 10
41-50 5 - -
RESULT & DISCUSSION:
a) Efficacy: Table 3: Efficacy

Treatments Doses/ha Observation at different days after 2nd application Yield of

PDI of Cercospora leaf spot PDI of Colletotrichum leaf spot Average population of mites/leaf dry chilli (q/Ac*)

g or ml 7th 15th 21st
7th 15th 21st 5th 10th 15th
Mancozeb 45% + Propargite 20% WP 3000 5.14 7.12 7.24 7.45 8.28 10.20 0.80 1.13 2.30 23.09
Mancozeb 40% +
Propargite
15%WP 3000 5.32 7.68 8.16 7.52 8.40 10.42 0.93 1.17 2.40 22.63
Mancozeb 38% + Propargite 12% WP 3000 6.11 9.66 11.00 9.61 9.90 14.66 1.27 3.73 4.27 17.56
Mancozeb 75% WP 2000 5.68 9.58 11.18 9.53 11.88 14.86 1.93 3.67 4.87 18.10
Propargite 57% EC 1500 7.00 10.88 18.64 9.88 14.66 19.80 0.80 1.26 2.30 17.00
Control - 7.66 11.00 19.88 10.40 14.80 20.80 1.93 3.67 5.00 12.04
CD @ 5% - 0.68 1.80 2.30 0.63 1.40 2.82 NS 1.15 0.82 3.43
quintal per acre
The data in Table -3 shows per cent disease index for Cercospora and Colletotrichum leaf spot of various treatments of (Mancozeb + Propargite WP) and individual components Mancozeb 75% WP and Propargite 57% EC. The disease severity was more in case of untreated control and Propargite 57% EC treated plots. Combination treatment of Mancozeb + Propargite showed better efficacy against leaf spot disease

and mites as compared to that of high concentration of Mancozeb or Propargite alone. Further, it was found that amongst the combinations of Mancozeb and Propargite at various proportions, the combination of Mancozeb 45% + Propargite 20% in wettable powder form shows higher efficacy against leaf spot diseases and mites. It was also observed that Mancozeb alone was effective only beyond 75% whereas Propargite alone was effective beyond 57%. This means that applying Mancozeb 45% alone and Propargite 20 % alone were at the most effective as that of the control.
Yield :
Application of composition of the present disclosure (Mancozeb + Propargite WP) significantly enhanced the crop yield (Table-3). Among all the treatments Mancozeb 45% + Propargite 20% WP recorded maximum grain yield (23.09 q/Ac) followed by Mancozeb 40% + Propargite 15% WP (21.63 q/Ac) respectively. Whereas Mancozeb 75% WP (18.10 q/Ac) and Propargite 57% EC (17.00 q/Ac) alone were comparatively less effective than combinations.
Phytotoxicity :
Table 2. Observations for phytotoxicity of Mancozeb + Propargite WP on chilli

Sr. Treatments Dose Leaf Injury Necrosis Wilting Vein Clearing Epinasty Hyponasty
No
(g/ ha) Observation at Days after application

0 3 7 10 0 3 7 10 0 3 7 10 0 3 7 10 0 3 7 10 0 3 7 10
Mancozeb
1. 45% + Propargite 20% WP 6000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mancozeb
2. 40% + Propargite 15% WP 6000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mancozeb 38
3. % + Propargite 12% WP 6000 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Control
4. Water spray 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Observation recorded in Table-4 showed that at double dose different combinations of (Mancozeb + Propargite) WP did not cause any phytotoxic effect on Chilli crop.
CONCLUSION:
Considering the efficacy on both the pests i.e. Leaf spot and Mites at the dose rate of 3000 g formulation/ha , it can be concluded that Mancozeb 45% + Propargite 20% WP was the best treatment whereas the next best treatment was Mancozeb 40% + Propargite 15% WP. This is due to the fact that each component complements the action of other and thus shows synergy.
ECONOMIC SIGNIFICANCE AND TECHNICAL ADVANCEMENT
The synergistic composition of the present disclosure has the following advantages:
- It reduces the cost on account of use of lesser amount of Mancozeb/Propargite as compared to individual use.
- Fungi and mites can be controlled in a single application. This reduces the labor cost and time consumption.
- Due to the combined application it exhibits enhanced efficacy.
Throughout this specification the word "comprise", or variations such as "comprises" or "comprising", will be understood to imply the inclusion of a stated element, integer or step, or group of elements, integers or steps, but not the exclusion of any other element, integer or step, or group of elements, integers or steps.
The use of the expression "at least" or "at least one" suggests the use of one or more elements or ingredients or quantities, as the use may be in the embodiment of the invention to achieve one or more of the desired objects or results.
Any discussion of documents, acts, materials, devices, articles or the like that has been included in this specification is solely for the purpose of providing a context for the invention. It is not to be taken as an admission that any or all of these matters form

part of the prior art base or were common general knowledge in the field relevant to the invention as it existed anywhere before the priority date of this application.
The numerical values mentioned for the various physical parameters, dimensions or quantities are only approximations and it is envisaged that the values higher/lower than the numerical values assigned to the parameters, dimensions or quantities fall within the scope of the disclosure, unless there is a statement in the specification specific to the contrary.
While considerable emphasis has been placed herein on the product and the process, it will be appreciated that alterations can be made and that many modifications can be made in the preferred embodiment departing from the principles of the disclosure. These and other changes in the preferred product and the process of the disclosure will be apparent to those skilled in the art from the disclosure herein, whereby It is to be distinctly understood that the foregoing descriptive matter is to be interpreted merely as illustrative of the disclosure and not as a limitation.

We Claim:
1. A synergistic biocidal composition comprising:
a. Propargite in an amount ranging between 2 % and 35 % with respect to the
total mass of the composition; and
b. Mancozeb in an amount ranging between 30 % and 80 % with respect to
the total mass of the composition,
wherein the proportion of Propargite to Mancozeb ranges between 1:1 and 1:40
2. The synergistic biocidal composition as claimed in claim 1; further comprises at least one excipient selected from the group consisting of surfactant, stabilizer, filler, solubilizer, binding agent and defoaming agent, wherein the total amount of the excipient ranges between 0.5 % and 40 % with respect to the total mass of the composition.
3. The synergistic biocidal composition as claimed in claim 2; wherein the surfactant is at least one selected from the group consisting of sodium ligno sulphonate, modified sodium lignosulfonate, sulfonated naphthalene condensate sodium salt, sodium alkyl naphthalene sulphonate, blend of Sodium alkyl naphthalene sulphonate and anionic surfactant, sulfonated aromatic polymer sodium salt, sodium lauryl sulphate, blend of anionic surfactants belonging to class of naphthalene sulphonate, sodium salt of dibutyl naphthalene sulphonate, blend of alcohol alkoxylate and fatty alcohol ethoxylates and sodium salt of polycarboxylic acid, in an amount ranging between 5 % and 15 % with respect to the total mass of the composition.
4. The synergistic biocidal composition as claimed in claim 2; wherein the stabilizer is at least one selected from the group consisting of hexamethylene tetra amine and sodium meta bisulphite, in an amount ranging between 0.5 % and 5% with respect to the total mass of the composition.

5. The synergistic biocidal composition as claimed in claim 2; wherein the filler is at least one selected from the group consisting of silica and kaolin clay, in an amount ranging between 1 % and 30% with respect to the total mass of the composition.
6. The synergistic biocidal composition as claimed in claim 2; wherein the solubilizer is at least one selected from the group consisting of calcium salt of alkyl benzene sulphonates, castor oil ethoxylates, sulfonated natural oils and fats, sulfonate esters and alkyl phenol ethoxylates, in an amount ranging between 1 % and 10% with respect to total mass of the composition.
7. The synergistic biocidal composition as claimed in claim 2; wherein the binding agent is at least one selected from the group consisting of dextrose and poly vinyl alcohol, in an amount ranging between 0.5% and 5% with respect to the total mass of the composition.
8. The synergistic biocidal composition as claimed in claim 2; wherein the defoaming agent is poly dimethyl siloxane in an amount ranging between 0.5 and 5% with respect to the total mass of the composition.
9. The synergistic biocidal composition as claimed in claim 1, wherein the composition is in the form selected from the group consisting of aerial sprays, directly sprayable solutions,, wettable powders, suspensions, dispersions, emulsions, oil dispersions, pastes and wettable granules.
10. The synergistic biocidal composition as claimed in claim 1 is in a wettable powder form; said composition comprising:
a. Propargite in an amount ranging between 10 % and 25 % with respect to the
total mass of the composition,
b. Mancozeb in an amount ranging between 30 % and 45 % with respect to
the total mass of the composition,

c. at least one surfactant selected from the group consisting of sodium ligno
sulphonate, modified sodium ligno sulfonate, sulfonated naphthalene
condensate sodium salt, sodium alkyl naphthalene sulphonate, blend of
Sodium alkyl naphthalene sulphonate and anionic surfactant, sulfonated
aromatic polymer sodium salt, sodium lauryl sulphate, blend of anionic
surfactants belonging to class of naphthalene sulphonate, sodium salt of
dibutyl naphthalene sulphonate, blend of alcohol alkoxylate and fatty
alcohol ethoxylates, in an amount ranging between 5 % and 15 % with
respect to the total mass of the composition
d. at least one filler selected from the group consisting of silica and kaolin
clay, in an amount ranging between 1 % and 30 % with respect to the total
mass of the composition,
e. at least one solubilizer selected from the group consisting of calcium salt of
alkyl benzene sulphonates, castor oil ethoxylates, sulfonated natural oils
and fats, sulfonate esters, alkyl phenol ethoxylates, in an amount ranging
between 1 % and 5 % with respect to the total mass of the composition, and
f. optionally, at least one stabilizer selected from the group consisting of
hexamethylene tetra amine and sodium meta bisulphite, in an amount
ranging between 0.5 % and 5 % with respect to the total mass of the
composition.
11. The synergistic biocidal composition as claimed in claim 1 is in a wettable granule form; said composition comprising:
a. Propargite in an amount ranging between 10 % and 25% with respect to the
total mass of the composition,
b. Mancozeb in an amount ranging between 30 % and 45 % with respect to
the total mass of the composition,
c. at least one surfactant selected from the group consisting of sodium ligno
sulphonate, modified sodium lignosulfonate, sulfonated aromatic polymer
sodium salt, sodium salt of polycarboxylic acid, sodium alkyl naphthalene

sulphonate, sodium salt of dibutyl naphthalene sulphonate, sodium alkyl naphthalene sulphonate, in an amount ranging between 5 % and 15 % with respect to the total mass of the composition,
d. at least one filler selected from the group consisting of. silica and kaolin
clay, in an amount ranging between 1 % and 30 % with respect to the total
mass of the composition,
e. at least one stabilizer selected from the group consisting of hexamethylene
tetra amine and sodium meta bisulphite, in an amount ranging between 0.5
% and 5 % with respect to the total mass of the composition,
f. at least one binding agent selected from the group consisting of dextrose,
poly vinyl alcohol, in an amount ranging between 0.5 % and 5 % with
respect to the total mass of the composition,
g. dimethyl siloxane as a defoaming agent, in an amount ranging between 0.5
% and 5 % with respect to the total mass of the composition, and
h. water in an amount ranging between 40% and 55 % with respect to the total mass of the composition.
12. A kit comprising;
i. Propargite as a component A;
ii. Mancozeb as a component B; and
iii. at least one packaging material for packaging the components (A) and (B).
13. The kit as claimed in claim 13, further comprises at least one excipient selected from the group consisting of surfactant, stabilizer, filler, solubilizer, binding agent and defoming agent.
14. A process for preparing a synergistic biocidal composition in the form selected from the group consisting of aerial sprays, directly sprayable solutions, wettable powders, suspensions, dispersions, emulsions, oil dispersions, pastes and wettable granules; said process comprising at least one step selected from

the group consisting of mixing, pulverizing, blending, wet milling, spray drying and extruding.
15. The process for preparing a synergistic biocidal composition in the form of
wettable powder as claimed in claim 14, said process comprising the following
steps:
a. mixing Propargite with at least one excipient selected from the group
consisting of surfactant, solubilizer, filler and stabilizer to obtain a
mixture;
b. pulverizing the mixture in a pulverizer to obtain a pulverized mixture
containing 2 to 7 micron sized particles; and
c. blending the pulverized mixture with Mancozeb or Mancozeb mixture
containing at least one excipient selected from the group consisting of
surfactant, solubilizer, filler and stabilizer to obtain a synergistic
biocidal composition in a wettable powder form.
16. The process for preparing a synergistic biocidal composition in the form of
wettable granules as claimed in claim 14, said process comprising the
following steps:
a. mixing Mancozeb, at least one excipient selected from the group
consisting of surfactant, filler, binding agent, defoaming agent and
stabilizer, and water to obtain a slurry having total solids in the range
of 40 to 55%;
b. wet milling the slurry to obtain wet milled slurry containing 2 to 7
micron sized particles;
c. blending the wet milled slurry with Propargite or Propargite mixture
containing at least one excipient selected from the group consisting of
surfactant, stabilizer, filler, binding agent and defoaming agent to
obtain slurry having total solid content in the range of 45 % to 60% and

d. spray drying the slurry to obtain a synergistic biocidal composition in a wettable granules form having particle size in the range of 100 to 1000 micron.
17. The process for preparing a synergistic biocidal composition in the form of wettable granules as claimed in claim 14, said process comprising the following steps:
a. mixing Propargite with at least one excipient selected from the group
consisting of surfactant, stabilizer, filler, binding agent and defoaming
agent to obtain a mixture;
b. pulverizing the mixture in a pulverizer to obtain a pulverized mixture
containing 2 to 7 micron sized particles;
c. blending the pulverized mixture with Mancozeb and water;
d. extruding the mixture through an extruder to obtain a synergistic
biocidal composition in a wettable granules form having particle size in
the range of 100 to 2000 microns.