Field of the invention
The present invention relates to a cosmetic composition comprising combination of
herbal aqueous extracts prepared from Tecomella undulata and Cephalandra indica that
provides both anti-oxidant activity with enhanced protection against free radicals and
anti-ageing activity with improved collagen induction.
Background of the invention
In pharmaceutical and personal care industries, there is an everlasting quest for high-
performing, potent, safe and inexpensive actives. In this context, many anti-oxidant and
anti-ageing actives are known in the art. The source of the actives could be synthetic or
natural, could be plant-based or animal-based, and could be derived through simple
methodology or through multi-step processes. Actives of herbal origin which exhibit
multi-functionality usually become a formulator's preferred choice due to the expected
safety and biocompatibity aspects.
Excessive free radicals are associated with a variety of problems and disease conditions
related to oxidative damage. In the context of skin care, excessive free radicals have
been known to accelerate the ageing process resulting in degradation of extracellular
matrix proteins such as collagen, elastin, etc manifesting in wrinkles, loss of elasticity,
etc. Excessive free radicals also impair the ability of the cell to protect itself from the
harmful UV rays affecting the melanin production pathway. Anti-oxidants empower the
body to fight against oxidative damage by scavenging excessive free radicals.
Choudhuryet.al. (Phytochemistry of the Family Bignoniaceae- A review.
ShuvasishChoudhury, SuparnaDatta, Anupam Das Talukdar,
ManabendraDuttaChoudhury. Biological and Environmental Sciences: 7(I), 145-150,
2011) teaches that the Bignoniaceae family of plants containing phyto-compounds like
flavonoids and phenolic acids that have been reported to have multiple biological effects
including antioxidant activity. The most common natural antioxidants are flavonoids

and phenolic compounds which are widely available from these families of plants and
are one of the important sources of new bioactive compounds.
Paula et.al. (Antioxidant activity from the leaf extracts of Jacaranda
puberulaCham., Bignoniaceae, a Brazilian medicinal plant used for blood
Depuration. Paula MacedoLessa dos Santos, Alberto SaddaJapp,
LiviaGralatoLimaJanSchripsema, Fabio de Sousa Menezes, Ricardo Machado
Kuster.Brazilian Journal of Pharmacognosy: 20(2), 147-153, 2010) discloses the
antioxidant activity of the dry extract of Jacaranda puberula, a Bignoniaceae family of
plant. In this article authors have shown that the extracts showing higher antioxidant
activities having strong absorption at 254 nm and 278 nm implicating presence of
chromospheres which are phenolic compounds.
Kumar et.al. (In vivo anti-hyperglycemic and antioxidant potentials of ethanolic
extract from Tecomellaundulata. Suresh Kumar, Sunil Sharma, NeeruVasudeva and
VarunRanga. Diabetology& Metabolic Syndrome: 4, 33, 2012) discloses both in vitro
and in vivoantioxidant activity of Tecomellaundulataindiabetic rat model. The article
discloses the in-vivo anti-hyperglycemic and antioxidant potentials of ethanolic extract
of leaves of Tecomella undulata. This prior art further discloses that there is an evidence
that glycosylationof various protein may itself induce the generation ofoxygen-derived
free radicals in diabetic condition. Hyperglycemia results in the generation of free
radicalwhich can exhaust antioxidant defense thereby leadingto the disruption of
cellular function,oxidativedamageto membrane and enhance the susceptibility to lipid
peroxidationand diffuse from the site of tissue damagewhich is measured by
malondialdehyde level (MDA). Kumaret.al. also disclose thatTecomella undulata
extract significantlydecreases lipid peroxidation in diabetic rats. It is postulated that
marked reduction in plasmaMDA level is a result of both direct antioxidant action and
glycemic control of Tecomella undulata extract.
Deshpande et.al. (Study on antioxidant activity of fruit extracts of Cocciniagrandis
L. voigt. Deshpande S.V, Patil M.J., Parmar K.K, Daswadkar S.C and Khodade R.B.

International Journal of Drug Research and Technology:1 (1), 69-72, 2011) teach
antioxidant activity of Cocciniagrandis, the methanolic extract of the fruit of
Cocciniagrandis showed significant antioxidant activity. The antioxidant activity of the
fruit may be attributed to the presence of flavonoids and anthraquinone glycosides.
Cocciniagrandis fruit also possess reducing power ability, free radical scavenging
activity when compared with standard, butylatedhydroxyanisole.
Mukherjee et.al. (Bioactive compounds from natural resources against skin aging.
Pulok K. Mukherjee, NiladriMaitya, Neelesh K. Nemaa, Birendra K. Sarkarb.
Phytomedicine: 19, 64-73, 2011) discloses the anti-ageing activity of Cucurbitaceae
family of herbal plants. It further discloses that 1,2,4,6-Tetra-O-galloyl-glucopyranose
and gallic acid isolated from M. heterophylla, a family of Cucurbitaceae herbal plant
known to play an important role over Matrix metalloproteinases (MMPs) expression in
photo-ageing by mediating the degradation of extracellular matrix proteins, but no
inhibition of MMP-1 mRNA expression. Both these compounds significantly inhibited
MMP-1 expression at the protein level and have a potent anti-oxidant activity
suggesting the usefulness as an anti-photoageing agent. Also, Cucumissativus L.
(Family: Cucurbitaceae) fruit has been found to possess in vitro inhibition of
hyaluronidase and elastase and MMP-1, which suggest the potential of this plant as anti-
wrinkle product.
There are many formulations available in the market which comprise of chemicals
acting as anti-ageing agents. However such chemicals may have harsh effects on skin
and accordingly there is a need to develop a herbalcomposition; specifically a herbal
composition which can be used to treat signs of ageing such as wrinkles, fine lines,
uneven skin tone, etc.
Herbal compositions disclosed in the art are mostly extracted in organic solvents.
Unfortunately such organic extracts are often incompatible with all formats of cosmetic
products such as water based serums, gels and concentrates as they are usually difficult
to solubilize in product like serums and hydrogels where the composition comprises of

greater than 85% water. An aqueous extract is thus preferred as theywill not have
interference from residual extraction solvent and therefore are considered safer as
compared to organic solvent extracts.
While there are many actives having anti-oxidant activity, may not show anti-ageing
activity. The present inventors have found that Argyreia speciosa extract shows anti-
oxidant activity, but failed to exhibit anti-ageing activity (figure 1). Thus there is a need
to develop a herbal composition which comprises aqueous extracts of herbal actives
offering both anti-oxidant and anti-ageing activities. The present invention provides a
unique combination of two plant extracts providing both anti-oxidant and anti-ageing
activities and thus solves the problem of unavailability of both the functions offered by
single formulation.
Object of invention
It is an object of the present invention to overcome the drawbacks of the prior art.
It is another object of the present invention to provide a combination of herbal extracts
that provides both anti-oxidant and anti-ageing activities.
It is yet another object of the present invention to provide a composition comprising
combination of Tecomella undulata and Cephalandra indica extracts providing both
anti-oxidant and anti-ageing activities.
Summary of invention
A cosmetic composition comprising aqueous extracts of Tecomella undulata and
Cephalandra indica; and cosmetically acceptable excipients; wherein said extracts are
present in a ratio of about 1:1.

Brief description of accompanying drawings:
Figure 1 illustrates the anti-oxidant activity and anti-ageing activity of Argyreia
speciosa extract at different concentrations.
Figure 2a illustrates the percent scavenging activity due to synergistic effect of herbs at
10000ppm concentration at 1:1 ratio through DPPH assay and is represented by bar
diagrams. Tecomella undulata extract is represented as TU and Cephalandra indica
extract is represented as Cpl.
Figure 2b illustrates the percent scavenging activity due to synergistic effect of herbs at
1000ppm concentration through DPPH assayand is represented by bar diagrams.
Tecomella undulate extract is represented as TU and Cephalandra indica extract is
represented as Cpl.
Figure 2c illustrates the percent scavenging activity due to synergistic effect of herbs at
100ppm concentrationthrough DPPH assayand is represented by bar diagrams.
Tecomella undulata extract is represented as TU and Cephalandra indica extract is
represented as Cpl.
Figure 2d illustrates percent scavenging activity due to synergistic effect of herbs at
10ppm concentrationthrough DPPH assayand is represented by bar diagrams. Tecomella
undulata extract is represented as TU and Cephalandra indica extract is represented as
Cpl.
Figure 3 illustrates theestimation of total collagen contents bySircol collagen assay and
is represented by bar diagrams. Tecomella undulata extract, represented as TU and
Cephalandra indica extract, represented as CpI at 10 ppm concentration.
Detailed description of the invention:

The present invention relates to cosmetic composition comprising acombination of
herbal extracts that provides both anti-oxidant and anti-ageing activity.
The present inventors have found that a combined herbal extract preparation from
Tecomella undulata and Cephalandra indica provides enhanced protection against free
radicals (anti-oxidant activity) as well as provides an enhanced collagen induction (anti-
ageing activity).
According to a preferred embodiment of the present invention, the extract of these two
plants are used together in pre-determined ratios over a specific concentration range
providing both enhanced anti-oxidant activity and anti-ageing activity.
The present invention provides that the concentration of the herbal extract combination
is 1000ppm, preferably 100ppm and more preferably 10ppm and the ratio of the herbal
actives is aboutl:l.
The herbal extracts of the present invention are aqueous in nature.To prepare the said
extracts, stem of Tecomella undulate and fruits of Cephalandra indica, were used.
The present invention further relates to cosmetic compositions which is preferably
topical skin care compositioncomprising combination of aqueous extracts of stem
ofTecomella undulata and fruits of Cephalandra indica and further cosmetically
acceptable excipients.
The cosmetically acceptable excipients can be selected from silicones, vitamin and/or
derivatives thereof, polyols, vehicles, structurants, emollient/s, gelling agent/s, a
thickening agent/s, a hydrophilic or hydrophobic polymer/s, an emulsifying agent/s,
alcohol/s etc. Examples of these ingredients include but are not limited to substances as
binders, emollients, preservatives (such as methyl paraben), colorants, perfumes, skin-
lightening agents, anti-wrinkle agents, antimicrobials and the like.

The silicone used in the invention is selected from linear, branched, crosslinked,
silicone oils, volatile and non volatile silicones such as dimethiconecopolyol,
dimethylpolysiloxane, diethylpolysiloxane, high molecular weight dimethicone, mixed
C1-C30 alkyl polysiloxane, phenyl dimethicone, dimethiconol, cyclopentasiloxane,
dimethicone, dimethiconol, mixed C1-C30 alkyl polysiloxane, and mixtures thereof.
The concentration of silicones is about 0.01 to 5.0 wt%; preferably about 0.1 to 3.0
wt%, more preferably about 0.5 to 2.0 wt%.
The polyols used are selected from glycerol, ethylene glycol, propylene glycol,
pentaerythritol, diglycerol, polyglycerol, their derivatives and combinations thereof. In a
preferred embodiment the invention relates to compositions wherein the concentrations
of polyol compounds varies from about 0.1 to 10.0 wt%; preferably between 0.5 and 5.0
wt%, most preferably between 1.0 and 4.0 wt%.
The vitamin used in the composition (optional) is selected from a group comprising
vitamin A, vitamin B (1-12), vitamin C, vitamin D (2-4), vitamin E, vitamin K, their
derivatives, such as acetates, propionates, palmitates, phosphates, alone on in
combinations thereof. The concentrations of the vitamin and/or its derivative(s) may be
about 0.01 to 2.5wt%; preferably about 0.05 and 2.0wt%, and more preferably between
0.5and1.5wt%.
Emulsifying agents which may be optionally added to the compositions of the present
invention include but are not restricted to oxyalkylenated fatty acid esters of polyols, for
example polyethylene glycol stearates, for instance PEG-100 stearate, PEG-50 stearate
and PEG-40 stearate; and mixtures thereof, mixture of glycerylmonostearate and of
polyethylene glycol stearate (100 EO) (Simulsol 165), oxyalkylenated fatty acid esters
of sorbitan comprising, for example, from 20 to 100 EO such as Tween 20 or Tween 60,
oxyalkylenated (oxyethylenated and/or oxypropylenated) fatty alcohol ethers;
alkoxylated or non-alkoxylated sugar esters, such as sucrose stearate and PEG-20
methylglucosesesquistearate; sorbitan esters such as the sorbitanpalmitate (Span 40),
esters of diacid and of fatty alcohol, such as dimyristyl tartrate; mixtures of these

emulsifiers, for instance the mixture of glyceryl stearate and of PEG-100
stearate(Arlacel 165), and mixtures comprising these emulsifiers, such as the mixture of
dimyristyl tartrate, cetearyl alcohol, Pareth-7 and PEG-25 laureth-25, (Cosmacol PSE),
steareth 2, steareth 21, PPG-15 stearyl ether.
Thickeners which may be used in the instant invention include but are not restricted to
alkyloamides, carbomer 934,940,941,960,961, cetearyl alcohol, cetyl alcohol, gelatin,
gums, magnesium aluminium silicates, ozocarite, paraffin, tragacanth, sodium alginate,
Tinovis ADM and the like.
Structurants which may be used in compositions of the present invention include those
materials which are well known in the art and include fatty acids, fatty alcohols, fatty
acid esters, and fatty acid amides, having fatty chains of from 8 to 30 carbon atoms.
Preferably the structurant used is stearic acid.
Various emollients as are known to a person skilled in the art and are available in the
market can be employed as an optional constituent in the instant invention. These
include but are not restricted to the group consisting of lanolin, octyldodecanol, hexyl
decanol, oleyl alcohol, decyloleate, isopropyl stearate, isopropyl palmitate, isopropyl
myristate, hexyl laureate, dioctyl cyclohexane, PPG-15 stearyl ether, isohexadecane,
stearic acid, cetyl alcohol, mineral oil etc.
Various chelating agents may be used as optional constituents of the instant invention.
These may be selected from a group consisting of but not limited to Dimercapto-
succinic acid (DMSA), Dimercapto-propane sulfonate (DMPS), Alpha lipoic acid
(ALA), Calcium disodium versante (CaNa2-EDTA), Disodium EDTA, Dimercaprol
(BAL).
Various cosmetically and dermatologically suitable preservatives may be added to the
instant composition. These may be selected from the group consisting of but not
restricted to 2-phenoxyethanol, para-hydroxybenzoic acid esters, also known as

parabens, for instance methyl para-hydroxybenzoate (methyl paraben), ethyl para-
hydroxybenzoate (ethyl paraben) and propyl para-hydroxybenzoate (propyl paraben)
and mixtures thereof; formaldehyde-releasing agents, for instance imidazolidinylurea or
diazolidinylurea; haloalkynylcarbamates, for instance 3-iodo-2-propynyl butyl
carbamate (IPBC); caprylyl glycol, also known as 1,2-octanediol; sodium benzoate;N-
(3-chloroallyl)- hexaminiumchloride (orQuaternium-15); polyhexamethylenebiguanide
hydrochloride (CTFA name: polyaminopropylbiguanide); alkyltrimethylammonium
bromides, for instance dodecyltrimethylammonium bromide,
myristyltrimethylammonium bromide and hexadecyltrimethylammonium bromide, and
mixtures thereof.
Anti-ageing active agents which may optionally be added to the compositions of the
instant invention may be chosen from free-radical scavengers, keratolytic agents,
vitamins, anti-elastase and anti-collagenase agents, proteins, fatty acid derivatives,
steroids, trace elements, bleaching agents, algal and plankton extracts, enzymes and
coenzymes, flavonoids, ceramides, tensioning agents and muscle relaxants, and
mixtures thereof.
Free-radical scavengers and antioxidants which may be optionally added to the
compositions of the instant invention include but are not restricted to phosphonic acid
derivatives such as ethylenediaminetetra (methylenephosphonic acid), methylene
phosphonic acid, methylenephosphonic acid and salts thereof, in particular the sodium
salts thereof; ethylenediaminetetraacetic acid and its salts, such as the sodium salt;
guanosine; superoxide dismutase; tocopherol (vitamin E) and its derivatives (acetate);
ethoxyquine; lactoferrin; lactoperoxidase, and nitroxide derivatives; superoxide
dismutases; glutathione peroxidase; plant extracts with free-radical-scavenging activity,
such as the aqueous extract of wheat-germ (Detoxiline), green tea, and mixtures thereof.
The composition of the invention may utilize a fragrance composition comprising a
blend of essential oils and synthetic aroma compounds. The blend is often diluted with a
carrier like propylene glycol, vegetable oil, or mineral oil. Some examples of synthetic

aroma compound which may be optionally added to the compositions of the instant
invention include but are not restricted to benzaldehyde, citral, vanillin, ethyl acetate,
fructone, octyl acetate, pentylbutanoate, pentylpentanoate, methyl salicylate, isoamyl
acetate, limonene, citronellol, and mixtures thereof.
Emotives that may be used in the present invention include but are not limited to
mentha, jasmine, camphor, white cedar, bitter orange peel, ryu, turpentine, cinnamon,
bergamot, citrus unshiu, calamus, pine, lavender, bay, clove, hiba, eucalyptus, lemon,
starflower, thyme, peppermint, rose, sage, sesame, ginger, basil, juniper, lemon grass,
rosemary, rosewood, avocado, grape, grapeseed, myrrh, cucumber, watercress,
calendula, elder flower, geranium, linden blossom, amaranth, seaweed, ginko, ginseng,
carrot, guarana, tea tree, jojoba, comfrey, oatmeal, cocoa, neroli, vanilla, green tea,
penny royal, aloe vera, menthol, cineole, eugenol, citral, citronelle, borneol, linalool,
geraniol, evening primrose, camphor, thymol, spirantol, penene, limonene, rose, lemon
grass, peach, honey, almond oil, olive oil, shea butter, olive butter, bearberry, black
currant, rosemary, blue lotus, white lily, and terpenoid oils.
Colourantsthat may be included in the composition of the present invention including,
but not limited to, inorganic, nitroso, monoazo, disazo, carotenoid, triphenyl methane,
triaryl methane, xanthene, quinoline, oxazine, azine, anthraquinone, indigoid,
thionindigoid, quinacridone, phthalocianine, botanical and natural colors, and
combinations thereof.
Various cosmetically acceptable vehicles may be used for the preparation of
compositions as per the instant invention. These may be selected from the group
consisting of but not restricted to water, alcohols, oils and combinations thereof.
Accordingly, when the surface contemplated is skin, the composition of this invention
may contain ingredients, which are added to creams, lotions, ointments, gels or
medicaments, which are physiologically acceptable to skin and may not contain
ingredients, which will reverse or retard the action of anti-oxidants/ anti-ageing

agent(s).
The present invention further relates to acosmetic composition comprising aqueous
extracts of Tecomella undulata and Cephalandra indica; and cosmetically acceptable
excipients; wherein said extracts are present in a ratio of about 1:1.
Thetopical skin care herbal extract is present in an amount of 10 ppm to 1000 ppm.
The topical skin care herbal aqueous extract of Tecomella undulata is present at a
concentration of 5 ppm to 500 ppm.
The topical skin care herbal aqueous extract of Cephalandra indica is present at a
concentration of 5 ppm to 500 ppm.
The topical skin care herbal aqueous extract of Tecomella undulata and Cephalandra
indica is present at a concentration of 10 ppm to 1000 ppm, preferably at a
concentration of 10 ppm to 100 ppm.
According to another embodiment, the present invention provides a process for
preparation of the extracts and cosmetic composition thereby.
The process of preparation of the extracts comprises the following steps: specific parts
of the plants are taken and powder was prepared after drying; these powders are added
to water which further steamed at a higher temperature followed by percolation or
extraction. Thus obtained concentrated extracts are distilled, filtered and dried under
vacuum to obtain the powdered extracts.
The present invention provides a process for preparation of cosmetic composition from
Tecomella undulata and Cephalandra indica extract comprises the steps of:

a. weighing the water soluble ingredients along with thickeners and mixing the
components in water to prepare a premix;
b. adding neutralizer to the premix of step (a) to obtain a neutralized premix;
c. weighing the oil soluble ingredients and melting the oil soluble ingredients to obtain
a molten oil phase;
d. homogenizing neutralized premix of step (b) with molten oil phase of step (c) to
obtain an emulsion formulation; and
e. adding extract of Tecomella undulata and Cephalandra indica, along with emotives,
colorants and fragrance to the emulsion of step (d) to obtain desired personal care
formulation of the present invention.
The present invention is now being illustrated by way of non-limiting illustrative
examples.
Example 1
Preparation of the herbal extract
The process for preparation of the extracts is as follows:
a. The plant material was thoroughly washed with tap water followed by distilled
water and dried at room temperature and was proceeded for preparation of plant
powders.
b. Preparation of plant powder:
Extract 1: The leaves of the plant Tecomella undulata were removed. The stem bark
of Tecomella undulata was cut into small pieces and ground to obtain powder. The
powder obtained was used for extraction.
Extract 2: The fruit of Cephalandra indica was grated into small pieces and dried at
room temperature for 48 hrs. The dried plant material was ground to a powder form.
1. 10 grams of these plant powders were added to 100 mL distilled water
2. The plant extract was steamed at a temperature ranging from 80°C - 100°C
followed by percolation or extraction for four cycles, each cycle for 4 hours

3. The concentrated extract was distilled for 10 to 12 hours
4. After distillation, the finer debris present in the extract was further separated
through filtration using a 40-60 mesh
5. The liquid paste was dried under vacuum at a temperature ranging from
80°C to 100°C for 2 to 4 hours. Dry powder format of herbal extract was
obtained.
Example 2
Determination of anti-oxidant activity of T. undulate (TU) and C. indica (Cpl)
aqueous extract through DPPH Assay
DPPH is one of the reliable methods for estimating antioxidant activity. The molecule
of 2, 2, diphenyl-1-picryl-hydrazyl (DPPH) is characterized as a stable free radical by
virtue of the derealization of the spare electron over the molecule as a whole so that the
molecules do not dimerise. The derealization also gives rise to deep violet color,
characterized by an absorption band at around 520nm.
Anti-oxidant assay of the herbal extract of T. undulate (TU) and C. indica (Cpl)
10µl of herbal extract of different concentrations (10ppm to 10000ppm)was added to
190µL of DPPH solution. L-Ascorbic acid was used as a positive control. Absorbance
was read against a suitable blank at 520 nm. The percent free radical scavenging activity
was determined.
In all the cases combined herbal extract shows improved anti-oxidant activity over a
wide range of concentration ranging from 10ppm to 10000 ppm and the effect is
prominent with decrease in the concentration (Figures 2a-2d), however prominent
synergism exists at concentrations ranging from 10 ppm to 1000 ppm.


* - Statistically significant; NS - Not significant; NA-Not applicable
It was found that the synergistic effect of T. undulate (TU) and C. indica (Cpl) extract at
ratio 1:1 possesses higher anti-oxidant activity compared to the individual herband
herbal actives mixed at ratios of 1:3 and 3:1 of TU and Cpl respectively (Table 1).
Example 3
Determination of anti-aging activity of T. undulata (TU) and C. indica (Cpl)
through Sircol collagen assay
The Sircol collagen assay is a dye-binding method designed for analysis of acid and
pepsin-soluble collagens and monitors collagen produced in-situ or during in-vitro cell
culture and in-vitro extracellular matrix. The assay can assess the rate of newly
producedcollagen i.e., the sircol dye reagent binds to the [Gly-X-Y]n helical structures
found on collagen, which is read spectrophotometrically at 555 nm.
Normal human dermal fibroblast [NHDF] cell lines were treated with actives for 48
hour and the collagen induction was estimated. The collagen produced was solubilized
in 200 µL of 0.5M acetic acid, followed by incubation with 1 mL Sircol dye. Excess dye

was removed by acid salt wash after which the collagen was dissolved in 250µL alkali
reagent. L-Ascorbic acid was used as a positive control. Absorbance was read against a
suitable blank at 555 nm. The amount of collagen produced (µg/well) was determined.
The results are depicted in figure 3 and represented in table 2 wherein the amount of
collagen was found to be higher due to the effect of herbal actives at ratio 1:1 when
compared to individual actives at a concentration of 10 ppm.
Table 2: Comparison of the anti-ageing activity of the combined aqueous herbal
extract with untreated control

Required quantity of water soluble ingredientsi.e. Xanthan gum, Disodium
EDTA, Magnesium sulfate, Phenoxyethanol, Ethylhexyl Glycerin, Ammonium
Acryloyl - dimethyltaurate / VP Copolymer Acrylates/C 10-30 Alkyl Acrylate
Cross-polymer, were weighed and mixed to prepare a premix in water by
heating them to 75 - 85 °C for about 15-20 minutes.
2. Triethanolaminewas added to the premix of step 1 to neutralize the same to
obtain a neutralized premix.
3. Oil soluble ingredients i.e. C12-15 Alkyl Benzoate, Potassium Cetyl Phosphate
and Hydrogenated Palm Glycerides, Cetostearyl alcohol, Glyceryl Stearate and
PEG-100 Stearate, Hydroxyethyl Acrylate / Sodium AcryloyldimethylTaurate
Copolymer andlsohexadecaneandPolysorbate 60 are weighed and melted the
same at 75 - 80 °C for 15 - 20 minutes to obtain a molten oil phase.
4. The neutralized premix of step 2 was homogenized with molten oil phase of step
3 at 55- 60 °C for 15 minutes to obtain an emulsion formulation.
5. Finally the extract of Tecomellaundulataand Cephalandraindicaand fragrance
was added to the emulsion of step 4 to obtain desired cream formulation.
Table 4: Topical skinserum withherbal aqueous extract comprising Tecomella
undulate and Cephalandra indica extract:



Process for preparation of serum:
1) EDTA, Glycerin, butylene glycol were weighed and mixed with water to form a
premix
2) Galaxy 2932, DL-a-Tocopherol Acetate and Allantoinwere solubilized in
Croduret 40 by heating at 40 °C for 10 mins. The mixture was brought to room
temperature before addition to the premix of step 1 to form solubilized premix.
3) Amaze XT, d-Panthenol, HydramolTM TGL ester were solubilized and added to
premix of step 2 and mixed for about 15-20 minutes.
4) Dow Corning 2501 cosmetic wax was added to mixture of step 3 followed by
phenoxetol, extracts and fragrance to obtain serum formulation.
Table5. Topical skinlotion withherbal aqueous extract comprising Tecomella
undulate and Cephalandra indica extract with lotion:



Process of preparation of lotion:
1. Required quantity of water soluble ingredients (Glycerine, Propylene Glycol,
Disodium EDTA, Phenoxyethanol, Hydrovance, PEG/PPG-8/3 Diisostearate,
silicones, Acrylates/ CI0-30 alkyl acrylate crosspolymer, Carbomer, Acrylates/
Acrylamide copolymer and mineral oil and polysorbate 85 and aqua)were weighed
and a premix was prepared in water by heating them to 75 - 85 °C for about 15-20
minutes.
2. Triethanolaminewas added to the premix of step 1 to neutralize the same to obtain a
neutralized premix.
3. Oil soluble ingredients likeOctylmethoxycinnamate , Steareth-21, Steareth-2, Stearic
acid, Cetyl Alcohol, Tocopheryl Acetate, C12-15 Alkyl Benzoate, Potassium Cetyl
Phosphate (and) Hydrogenated Palm Glycerides, Cetostearyl alcohol, Glyceryl
Stearate (and) PEG-100 Stearate, Hydroxyethyl Acrylate / Sodium
AcryloyldimethylTaurate Copolymer (and) Isohexadecane (and) Polysorbate 60
were weighed in required amount and melted at 75 - 80 °C for 15 - 20 minutes to
obtain a molten oil phase.
4. Thus obtained neutralized premix of step 2 was homogenized with molten oil phase
of step 3 at 55- 60 °C for 15 minutes to obtain an emulsion formulation.

5. Finally the extract of Tecomella undulate and Cephalandra indica and fragrance
were added to the emulsion of step 4 to obtain desired cream formulation.
Table 3, table 4 and table 5 describe the different topical skin care formulations in the
form of cream, serum and lotion, respectively.

WE CLAIM:
1. A cosmetic composition comprising aqueous extracts of Tecomella undulata and
Cephalandra indica; and cosmetically acceptable excipients; wherein said extracts
are present in a ratio of about 1:1.
2. The cosmetic composition as claimed in claim 1, wherein said aqueous extract of
Tecomella undulata is present at a concentration of 5 ppm to 500 ppm.
3. The cosmetic composition as claimed in claim 1, wherein said aqueous extract of
Cephalandra indica is present at a concentration of 5 ppm to 500 ppm.

4. The cosmetic composition as claimed in claim 1 or 2, wherein the said extract of
Tecomella undulate is the stem extract.
5. The cosmetic composition as claimed in claim 1 or 3, wherein the said extract of
Cephalandra indica is the fruit extract.
6. The cosmetic composition as claimed in any preceding claim,wherein cosmetically
acceptable excipients is selected from group comprising silicones, vitamin and/or
derivatives thereof, polyols, vehicles, structurants, emollients, gelling agents, a
thickening agents, a hydrophilic polymers hydrophobic polymers, an emulsifying
agents, alcohols and mixtures thereof.
7. A process for preparation of cosmetic composition of claim 1 said process
comprising:
a. weighing water soluble ingredients, thickeners and mixing saidwater soluble
ingredients, thickeners in water to form a premix;

b. adding neutralizer to the premix of step (a) to obtain a neutralized premix;
c. weighing oil soluble ingredients and melting said oil soluble ingredients to
obtain a molten oil phase;
d. homogenizing neutralized premix of step (b) with said molten oil phase of step
(c) to obtain an emulsion formulation; and
e. adding extracts of Tecomella undulata and Cephalandra indica, along with
emotives, colorants and fragrance to the emulsion of step (d).
8. The process for preparation of cosmetic composition as claimed in claim 7, wherein
said aqueous extract of Tecomella undulata is present at a concentration of 5 ppm to
500 ppm.
9. The process for preparation of cosmetic composition as claimed in claim 7, wherein
said aqueous extract of Cephalandra indica is present at a concentration of 5 ppm to
500 ppm.

ABSTRACT

A cosmetic composition comprising aqueous extracts of Tecomellaundulata and
Cephalandraindica; and cosmetically acceptable excipients; wherein said extracts are
present in a ratio of about 1:1. Thecombination of extracts present in an amount of 10
ppm to 1000 ppmprovides enhanced collagen induction and enhanced protection against
free radicals. Also provided is the process for preparation of the said cosmetic
composition.