The present invention relates to a synergistic fungicidal composition comprising an acylamino acid fungicide or its agrochemically acceptable salts; at least one fungicide selected from Strobilurin fungicides or their agrochemically acceptable salts and at least one fungicide selected from azole-based fungicides or their agrochemically acceptable salts. The present invention also relates to a process for preparation of such synergistic fungicidal composition.
BACKGROUND OF THE INVENTION
Fungicides are compounds of natural or synthetic origin, which act to protect plants against damage caused by fungi. Fungicides are used extensively in modern agricultural practices. In fact, some crops cannot be grown productively without the use of fungicides. Using fungicides allows a grower to increase crop yield and quality, and thus increase the crop value. In most cases, the increase in crop value is worth at least three times the cost of the fungicide.
Most crop and ornamental plants are subject to attack by several fungi. Plant diseases due to fungi cause significant productivity losses in ornamentals, vegetable, field, cereal and fruit crops, resulting in higher consumer cost. Plant diseases can be difficult to control and in addition to being highly destructive, may develop resistance to commercial fungicides. Due to the diversity of these organisms and their potential for inciting serious disorders in combination or alone has resulted in the need for broad-spectrum disease control. Although there are numerous chemical compounds (fungicides), available to aid in prevention of diseases of plants, each of these has practical deficiencies, which restrict its use. Fungicides combinations are frequently used to improve disease control, broaden the spectrum of control, and slow resistance development. Thus, new advantageous combinations are required to provide a variety of options to best meet specific plant disease control needs. Furthermore, certain uncommon fungicides combination exhibit a greater-than-additive (i.e. synergistic) effect to providing commercially significant levels of plant disease control.
When two or more substances in combination demonstrate unexpectedly high biological activity, for example fungicidal activity, the resulting phenomenon is known as synergism.
In order to achieve high crop productivity, it is important to protect the plant from damages caused by plant pathogenic (disease-causing) organisms.

Furthermore, there has been a need in the art to provide a synergistic fungicidal composition comprising two or more fungicides which have different mechanism of action that provide broad spectrum control while having lower rate of application and dose and yet providing enhanced efficacy and plant/crop yield. Moreover, improved rainfastness with such fungicidal composition comprising two or more fungicides has been identified in the prior arts. As a result, there has been a need to provide a fungicidal composition comprising with two or more fungicides that are stable with each other while still providing enhanced efficacy and synergistic effect over the solo formulations of the respective active ingredients.
Conventional fungicide compositions comprising acylamino acid fungicide with anti-peronosporic activity may not provide a practically sufficient control effect on plant diseases depending on the application situation, because it has an insufficient effect on specific plant diseases or its residual effectiveness lasts for a relatively short period of time. As a result, the range of fungal diseases and fungi that can be controlled and treated by acylamino acid fungicides is limited. In view of the above, developing a fungicidal combination that exhibits no cross-resistance to the existing fungicidal agents, no toxicity issues and little negative impact on the environment, while improving rainfastness and reducing pest resistance is extremely difficult.
Apart from the synergistic effect and increased bio-efficacy, it is also important to have a crop solution that entrusts resistance management -which frequently results in multiple pesticides due to repeated spray rounds during the single crop cycle, and to draw the dose optimization and cost per hectare (ha).
Various prior arts have offered various fungicidal compositions useful for protecting the plant from damage by harmful pests. Some of such prior arts are indicated below:
US20180160684, dislcoses a fungicide composition containing, as active ingredients, (a) Valifenalate or a salt thereof and (b) at least one fungicide selected from the group consisting of Isofetamid, Pyriofenone, and azole-based compounds; and a method for controlling a plant disease, which includes applying the fungicide composition to a plant or soil.
IN202034023458, discloses a fungicide composition comprising a multi-site fungicide for controlling phytopathogenic fungi in plant. Further it relates to use of said composition for the

controlling fungal infection and a method for the prevention and/or treatment of fungal leaf spot diseases caused by Zymoseptoria infection in plants.
EP2854547B1 discloses a composition comprising at least one biological control agent selected from specific microorganisms and at least one fungicide (I) which is Trifloxystrobin in a synergistically effective amount useful for reducing overall damage of plants and plant parts.
US2021007355A1, discloses combination of fungicides comprising at least one azole fungicides with other agro-chemically fungicides for controlling a broad spectrum of fungal diseases.
CN108353921A, discloses a kind of bactericidal composition including Valifenalate and Fenpyrazamine, the weight ratio of two kinds of active ingredient is 1- 40 : 40-1, the weight percentage of active ingredient is 1- 90% in fungicide, preferably 5- 80%, remaining is the auxiliary agent and carrier that allow in pesticide.
Fungicidal combinations have significant advantages over individual applications including improved and extended fungal control, lower fungicide application rates and costs, shorter contact times for improved results, less stringent use restrictions, improved selectivity, broader spectrum of fungi controlled, lower cost and fewer residue problems. However, determining appropriate fungicide application rates and combinations ratio is critical for disease control efficacy. There have been no studies to determine the most-effective and synergistic ratios of fungicides selected from acylamino acid class, at least one compound selected from strobilurin fungicide, and at least one compound selected from azole-fungicide, at which ratio these fungicides synergistically complement each other's fungicidal properties not seen at other ratios. Thus there exists a need in the art for a synergistic fungicidal composition comprising such fungicides.
Valifenalate, IUPAC name: methyl (3RS)-3-(4-chlorophenyl)-N-[N-(isopropoxycarbonyl)-L-valyl]-P-alaninate, anti-peronosporic fungicide, belonging to the group of Carboxylic acid (CAA) Amides, with strong systemic activity. Valifenalate belongs to acylamino acid class of fungicides. Fungi belonging to Oomycetes like Plasmopara viticola (grapevine), Phytotphthora infestans (potato and tomato), Peronosoora destructor (onion), PseudooeronosDora cubensis

(cucurbits), Bremia lactucae (lettuce and similar) are the exclusive target of Valifenalate (Valis). Valifenalate acts by inhibiting the cell metabolism and particularly the synthesis of the fungal cell wall. Particularly, it is an anti-peronosporic fungicide which is used to control mildew in many crops including grapes, potatoes and tomatoes.
CI

CH3 O X [
H3C CH3
Valifenalate
H3CT°T N I"N "^^^OCH=

Strobilurin fungicides are globally used to combat white mold, rot, early and late leaf spot, rusts and rice blast (Bartett et al., 2001; FAO Meeting, 2008). Mushrooms (Basidiomycetes) are natural sources of strobilurins, and the first natural strobilurin compound, Strobilurin-A, was originally isolated from the mushroom Strobilurus tenacellus by Anke et al. (1977). Examples of strobilurin fungicides, include Azoxystrobin, Pyraclostrobin, Fluoxastrobin, Kresoxim-methyl, Trifloxystrobin, Picoxystrobin, Mandestrobin, and Metominostrobin, which have been developed and marketed (Rodrigues et al., 2013; Khandelwal et al., 2014). Strobilurin fungicides are also referred as Qol (Quinine outside inhibition) fungicides because of their unique mechanism of action. They specifically bind to the quinol oxidation (Qo) site of cytochrome b to inhibit mitochondrial respiration. This binding blocks electron transfer between cytochrome b and cytochrome cl and inhibits the synthesis of nicotinamide adenine dinucleotide (NADH) oxidation and the mitochondrial membrane protein adenosine triphosphate (ATP). In particular, strobilurin fungicides work by inhibiting the fungi's ability to undergo normal respiration.
Various Strobilurin fungicides are known in the art. Chemical structure of known fungicides are provided below:



°^

A zoxystzobin

Pyraclostrobin

NT

Fluoxastrobin

K resoxim -m ethyl

OFXr%
CF

T riHo x y s tro b in

P icoxystrob in

Mandestrobin

Metominostrobin

Trifloxystrobin in particular is an Oximino- acetate fungicide with target site code C-3 and is classified as QoI-(quinone Outside inhibition) fungicide. Strobilurin fungicides have a broad spectrum of fungicidal activity, are cost effective, and degrade rapidly during plant metabolism. Such fungicides provide long-lasting, weather-protected disease control with high rainfastness.
Strobilurin fungicides are broad-spectrum with rapid and highly efficient fungicidal activities, cost effective and rapidly degrade during plant metabolism. Such fungicides provide long, lasting, weather, protected disease control having superior rainfastness capabilities.Strobilurin fungicides are effective in controlling variety of fungi and pests including control of Leptosphaerulina and Curvularia species.
Azole pesticide derivatives represent the most important category of fungicides that have excellent protective, curative and eradicant power towards a wide spectrum of crop diseases. The fungicide group, demethylation inhibitors (DMI), which contain the triazole fungicides,

was introduced in the mid-1970s. These fungicides are highly effective against many different fungal diseases, especially powdery mildews, rusts, and many leaf-spotting fungi. Various triazole fungicides are known in the art. Chemical structure of some of such fungicides are provided below:



"X),
oY
N^

Lriiklimefon

iL-ilLLi-ll.LA-.L-

propicoti azoic

mcii-iirwulc

1.-0
cfr

Kit
Q^I-CU £yX^ #fy£

C ifhi xir

mcpmnil

flulolanil

LlllJillll


Difenoconazole
Above mentioned fungicides can be formulated into a variety of different forms preferably into liquid and solid compositions.
The present invention relates to a novel synergistic composition which is stable as well as has required bio-efficacy, synergism as well as method of preparation and treating the plant that

provides excellent control over said diseases in plants and provides high yields, maintain nutrition and quality, health of the plants.
OBJECTS OF THE INVENTION:
It is an object of the present invention to provide a novel and effective fungicidal composition demonstrating high efficacy and high selectivity.
It is another object of the present invention to provide a novel and effective fungicidal composition for controlling the harmful pests and fungi in plants.
It is another object of the present invention to provide a novel and effective synergistic fungicidal composition which can be easily formulated.
It is another object of the present invention to provide a novel and effective synergistic fungicidal composition which is ideal for fungicide resistance management and enhanced disease control.
It is another object of the present invention to provide a novel and effective synergistic fungicidal composition that requires less of the active ingredientsas compared to the active ingredient alone.
It is another object of the present invention to provide a novel and effective synergistic fungicidal composition which is safe for environment, has broad spectrum bio-efficacy, is synergistic, is less toxic in terms of phytotoxicity.
It is another object of the present invention to provide a novel and effective fungicidal composition with excellent rain fastness.
Yet another object of the present invention is to provide a method of controlling fungi using fungicidal composition comprising fungicides from the acylamino acid class, at least one compound from strobilurin fungicide, and at least one compound from the azole class of fungicides.

Yet another object of the present invention is to provide a process for the preparation of novel and effective fungicidal composition.
SUMMARY OF THE INVENTION
Accordingly, the present invention provides a fungicidal composition comprising synergistic and bio-effective amount of fungicides selected from acylamino acid class or its agrochemically acceptable salts, and at least two agro chemically active fungicides or their agrochemically acceptable salts. Further, the present invention provides a stable synergistic fungicidal composition comprising valifenalate or its agro chemically acceptable salt thereof, at least one strobilurins fungicide, and at least one azole fungicide or their agrochemically acceptable salts.
In an embodiment of the present invention, the fungicidal composition comprises of acylamino acid fungicide having anti-peronosporic activity like Valifenalate, a strobilurin fungicide selected from but not limited to Azoxystrobin, Pyraclostrobin, Fluoxastrobin, Kresoxim-methyl, Trifloxystrobin, Picoxystrobin, Mandestrobin, or Metominostrobin; and an azole fungicide selected from but not limited to Imazalil, Oxpoconazole fumarate, Pefurazoate, Prochloraz, Triflumizole, Azaconazole, Bromuconazole, Bitertanol, Cyproconazole, Diclobutrazol, Difenoconazole, Diniconazole, Epoxiconazole, Etaconazole, Fenbuconazole, Fluquinconazole, Flusilazole, Flutriafol, Furconazole, Hexaconazole, Imibenconazole, Ipconazole, Metconazole, Myclobutanil, Penconazole, Propiconazole, Prothioconazole, Quinconazole, Simeconazole, Tebuconazole, Tetraconazole, Triadimefon, Triadimenol, or Triticonazole.
In another embodiment of the present invention, the fungicidal composition comprises of acylamino acid fungicide having anti-peronosporic activity and at least one fungicide selected from strobilurin fungicide and at least one another fungicide selected from azole fungicide, which are present in synergistically effective weight ratio.
In another embodiment of the present invention, Valifenalate or its agro chemically acceptable salt is present in an amount in a range of 1-60% by weight of the composition, the strobilurin fungicide or its agro chemically acceptable salt is present in an amount in a range of 1-40% by weight of the composition, and the azole fungicide or its agro chemically acceptable salt is present in a range of 5-50% by weight of the composition.

In an embodiment of the present invention, at least one azole fungicide is a triazole fungicide.
In another embodiment of the present invention, the composition comprises:
a. Valifenalate or its agrochemically acceptable salts in an amount in the range from 1 to
60% by weight of the composition;
b. at least one strobilurin fungicide or its agrochemically acceptable salts in an amount in
the range from 1 to 40% by weight of the composition; and
c. at least one azole fungicide or its agrochemically acceptable salts in an amount in the
range from 5 to 50% by weight of the composition.
In another embodiment of the present invention, the strobilurin fungicide is Trifloxystrobin, Picoxystrobin or Azoxystrobin, and the azole fungicide is diniconazole or difenoconazole.
In another embodiment of the present invention, the composition comprises of:
a. Valifenalate or its agrochemically acceptable salts in an amount in the range from 1 to
60%) by weight of the composition;
b. Trifloxystrobin or its agrochemically acceptable salts as strobilurin fungicide in an
amount in the range from 1 to 40% by weight of the composition; and
c. at least one azole fungicide or its agrochemically acceptable salts in an amount in the
range from 5 to 50% by weight of the composition.
In another embodiment of the present invention, the composition comprises of:
a. Valifenalate or its agrochemically acceptable salts in an amount in the range from 1 to
60%) by weight of the composition;
b. Trifloxystrobin or its agrochemically acceptable salts as strobilurin fungicide in an
amount in the range from 1 to 40% by weight of the composition; and
c. diniconazole, difenoconazole or its agrochemically acceptable salts in an amount in the
range from 5 to 50% by weight of the composition
In another embodiment of the present invention, the composition comprises of:
a. Valifenalate or its agrochemically acceptable salts in an amount in the range from 10 to 30%) by weight of the composition;

b. Trifloxystrobin or its agrochemically acceptable salts as strobilurin fungicide in an
amount in the range from 5 to 20% by weight of the composition; and
c. Difenoconazole or its agrochemically acceptable salts in an amount in the range from
5 to 25% by weight of the composition.
In preferred embodiment of the present invention, the composition comprises one or more agro chemically acceptable excipients.
In another embodiment of the present invention, the agrochemically acceptable excipients are selected from group comprising surfactants/dispersing agents, polymers, anti-freezing agent, wetting agents, anti-foaming agents, colorants, pigments, binder/structuring agents, biocides/anti-microbial agent, thickeners, emulsifiers, buffering agent, fillers, diluents/solvents, quick coating agents or sticking agents, preservatives, adjuvants and solvents or a combination thereof.
In another embodiment of the present invention, the thickeners are selected from the group comprising thickeners are selected from the group comprising silicates such metal silicates, aluminum magnesium silicate sodium carboxy methyl cellulose, methyl cellulose, ethyl cellulose, poly vinyl alcohol, sodium alginate, sodium poly acrylate, xanthan gum, welan gum, gum arabic, montmorillonite, lingo sulfonates, hydroxy methyl cellulose, dextrin, starch, Bentonite Clay, Polysaccharides or combinations thereof.
In another embodiment of the present invention, the anti-freezing agent is selected from the group comprising polyethylene glycols, methoxy polyethylene glycols, propylene glycol, polypropylene glycols, polybutylene glycols, Mono ethylene glycol, glycerin, ethylene glycol, Glycerin, Diethylene glycol or combinations thereof.
In another embodiment of the present invention, anti-foaming agent is selected from the group comprising silicone oils, or mineral oils, Fatty acid ester; potassium sorbate, and parahydroxy benzoates, silicon emulsion based anti-foam agents, Siloxane polyalkylene oxide, Polydimethyl Siloxane, tri siloxane ethoxylates or combinations thereof.
In another embodiment of the present invention, wetting agent is selected from the group comprising Tristyrylphenol ethoxylate nonionic emulsifier, Mixture of non-ionic surfactants &

Alkoxylated Alcohol/Block copolymer, sodium lauryl sulphate; sodium dioctyl sulphosuccinate; Tristyrylphenol ethoxylate non-ionic emulsifier/ mixture of non-ionic surfactants and alkoxylated Alcohol/Block copolymer, ionic surfactant mixtures, anionic surfactants, alkyl phenol ethoxylates, aliphatic alcohol ethoxylates and the salts or combinations thereof.
In yet another embodiment of the present invention, the polymers are selected from the group comprising polyvinyl alcohols, polyvinylpyrrolidone, gel-forming carraagheenans, water-soluble gelatine and casein, superabsorbent polymers such polyacrylamides and polyacrylates based polymers, semi-synthetic or fully-synthetic peptide/protein-based superabsorbent polymers such as collagen-based synthetic polymers, elastin-like polypeptides, polyaspartic acid, polyaspartates, polyglutamic acid, polyglutamate, Semi-synthetic or fully-synthetic polysaccharide: carboxymethyl starch , sulfoethyl starch, carboxymethyl cellulose, sulfoethyl cellulos, hydroxypropyl cellulose, hydroxyethyl cellulose, methylcellulose, chitosan, acrylonitrile, acrylic acid, methacrylic acid, acrylamide, methacrylamide, 2-acrylamido-2-methyl- propanesulfonic acid (AMPS), vinyl sulfonic acid, ethyl acrylate, and potassium acrylate or combinations thereof.
In yet another embodiment of the present invention, the adjuvant is selected from the group comprising Silicone Ethoxylated Oil, Polyvinyl Pyrrolidon, Poly vinyl Alcohol,Blend of poly terpene resin or combination thereof; the diluent/solvents are selected from the group comprising lactose, glucose, fructose, maltose, sucrose, in the anhydrous or hydrate forms, urea, water soluble or dispersible polymers, water soluble inorganic salts, Demineralized (DM) water, Vegetable Oil or combination thereof; and filer is selected from the group comprising Silicon Dioxide, China -Clay, Kaolin, Talc, starch or combination thereof.
In yet another embodiment of the present invention, the biocide/anti-microbial agent is selected from the group comprising Benzisothiazolin-3-one / Formaldehyde/Sodium benzoate/Sodium o-phenyl phenate, 5-chloro-2-methyl-4-isothiazolin-3-one & 2-methyl-4-isothiazolin-3-one or combination thereof.
In another embodiment of the present invention, the composition is in form of any one of as Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for

water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable Slurry (FS), Flowable Suspension(FS), Suspension Concentrate (SC), Suspension concentrate for direct application (SD), Suspo- emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Water soluble powder (SP), Water dispersible powder for slurry seed treatment (WS), Water dispersible granules (WDG), Wettable powders (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), granular (GR), oil in dispersion (OD), a mixed formulation of CS and SC (ZC) or a mixed formulation of CS and SE (ZE), a mixed formulation of CS and EW (ZW). In another preffered embodiment of the present invention, the preferred composition is in form of Suspension Concentrate (SC), Water dispersible granules (WG), Suspo- emulsion (SE), or Suspo- emulsion (SE).
In another embodiment of the present invention, it provides a method for controlling pests including fungi in plants, said method comprising applying to the locus of the pest infestation a fungicidal composition comprising acylamino acid fungicide having anti-peronosporic activity, at least one fungicide selected from strobilurin fungicide and at least one another fungicide selected from azole fungicide.
In another preffered embodiment of the present invention, it provides a method for improving vigour or yield of the plant comprising, treating a plant or portion thereof or soil, a fungicidally effective amount of the fungicidal composition.
In another embodiment of the present invention, the plant is selected from the group consisting of cereals, field crops, seeds, fruits, tree nuts, vegetables, turf grasses, potatoes, tomatoes, grapes and ornamentals.
In another embodiment of the present invention, the fungicidal composition of the present invention further comprises an agriculturally acceptable excipients selected from the group consisting of anti-freezing agent, dispersing agents, wetting agents, antifoaming agents, biocides, thickeners, surfactants and solvents. Additional components may also be included, e.g., protective colloids, adhesives, thickeners, thixotropic agents, penetration agents, stabilisers, sequestering agents. More generally, the active materials can be combined with any solid or liquid additive, which complies with usual formulation techniques.

In yet another embodiment of the present invention, the invention further provides the process for preparation of the said formulation wherein, the said formulation can be one or more of as Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable Slurry (FS), Flowable Suspension(FS), Suspension Concentrate (SC), Suspension concentrate for direct application (SD), Suspo- emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Water soluble powder (SP), Water dispersible powder for slurry seed treatment (WS), Water dispersible granules (WDG), Wettable powders (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), granular (GR), oil in dispersion (OD), a mixed formulation of CS and SC (ZC) or a mixed formulation of CS and SE (ZE), a mixed formulation of CS and EW (ZW).
In yet another embodiment of the present invention provides a kit-of-parts comprising a plurality of components, wherein said plurality of components comprises:
a. Valifenalate or its agrochemically acceptable salts;
b. at least two agro chemically active fungicides or their agrochemically acceptable salts;
and
c. at least one or more agrochemically acceptable excipients.
In yet another embodiment of the present, it provides process for preparation of a stable synergistic fungicidal composition as detailed above.
DETAILED DESCRIPTION OF THE INVENTION
Embodiments, of the present disclosure, will now be described herein. Embodiments are provided so as to thoroughly and fully convey the scope of the present disclosure to the person skilled in the art. Numerous details are set forth, relating to specific components, and methods, to provide a complete understanding of embodiments of the present disclosure. It will be apparent to the person skilled in the art that the details provided in the embodiments should not be construed to limit the scope of the present disclosure. In some embodiments, well-known processes, well-known additional excipients, and well-known techniques are not described in detail. Discussed below are some representative embodiments of the present invention. The invention in its broader aspects is not limited to the specific details and representative methods.

All technical and scientific terms used herein have the same meanings as commonly understood by someone ordinarily skilled in the art to which the present subject matter belongs.
The terminology used, in the present disclosure, is only for the purpose of explaining a particular embodiment and such terminology shall not be considered to limit the scope of the present disclosure. As used in the present disclosure, the forms "a," "an," and "the" may be intended to include the plural forms as well, unless the context clearly suggests otherwise. Thus, for example, reference to a composition containing "a compound" includes a mixture of two or more compounds. It should also be noted that the term "or" is generally employed in its sense including "and/or" unless the content clearly dictates otherwise. The terms "comprises," "comprising," "including," and "having," are open ended transitional phrases and therefore specify the presence of stated features, integers, steps, operations, elements, but do not forbid the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. The particular order of steps disclosed in the method and process of the present disclosure is not to be construed as necessarily requiring their performance as described or illustrated. It is also to be understood that additional or alternative steps may be employed.
Variations or modifications to the composition of this invention, within the scope of the invention, may occur to those skilled in the art upon reviewing the disclosure herein. Such variations or modifications are well within the spirit of this invention.
The expression of various quantities in terms of "% w/w" or "%" means the percentage by weight, relative to the weight of the total solution or composition unless otherwise specified. The term "active ingredient" (a.i.) or "active agent" used herein refers to that component of the composition responsible for control and killing of pest.
The terms "plants" and "vegetation" include, but are not limited to, germinant seeds, emerging seedlings, plants emerging from vegetative propagules, and established vegetation.
The term "crop" shall include a multitude of desired crop plants or an individual crop plant growing at a locus.

The term "synergistic", as used herein, refers to the combined action of two or more active agents blended together and administered conjointly that is greater than the sum of their individual effects.
"Bioactive// fungicidally effective amounts amounts" as mentioned herein means that amount which, when applied for treatment of crops, is sufficient to give effect in such treatment.
The present invention provides a stable synergistic composition comprising of a) valifenalate or its agro chemically acceptable salt thereof; and b) at least two agrochemically active fungicides.
More particularly, the present invention provides a composition comprising: (a) Valifenalate or its agro chemically acceptable salts; (b) at least one fungicide selected from Strobilurin fungicides or their agro chemically acceptable salts; and (c) at least one fungicide selected from azole fungicides or their agro chemically acceptable salts.
In a preferred embodiment of the present invention, the composition comprises of: (a) Valifenalate or its agro chemically acceptable salts; (b) at least one fungicide selected from Strobilurin fungicides or their agro chemically acceptable salts; (c) at least one fungicide selected from azole fungicides or their agro chemically acceptable salts and d) at least one or more agrochemically acceptable excipients.
In another preferred embodiment of the present invention, the composition comprises of:
a. Valifenalate or its agro chemically acceptable salt in a range of 1-60% by weight of the
composition;
b. at least one strobilurins fungicide or its agro chemically acceptable salt in a range of 1-
40% by weight of the composition;
c. at least one azole fungicide or its agro chemically acceptable salt in a range of 5-50%
by weight of the composition; and
d. at least one or more agro chemically acceptable excipients.
The present synergistic fungicidal composition combats effectively against the diseases of fruits, vegetables, cereals and oil seed crops such as Oomycetes - Plasmopara viticola causing

causes Powdery mildew of grapevines, Alternaria solani, Fusarium, Septoria in cereals, Pseudocercospora, Mycosphaerella, etc.
The fungicides of the present synergistic fungicidal composition when combined in a specified proportions, the dose per hectare (ha) is reduced and there is long duration of disease control. Further, the present synergistic fungicidal composition also exhibits better disease control during wet season due to its effective rainfastness property.
Accordingly, the present invention discloses a stable synergistic composition comprising 1-60 % w/w of valifenalate or its agro chemically acceptable salt, 1-40 % w/w of at least one strobilurin fungicide or its agro chemically acceptable salt,, 5-50 % w/w of at least one azole fungicide or its agro chemically acceptable salt, 0.5-10 % w/w dispersing agent, 0.5-10% wetting agent, 0.01-1 % w/w antifoaming agent, 0.1-10 % w/w anti freezing agetnt, 0.1-10 % w/w adjuants, 0.1-5 % w/w filler, 0.01-1 % w/w antibacterial/biocide, one or more of Polysaccharides, carboxymethyl cellulose, Bentonite Clay, Aluminum Magnesium Silicate in the range of 0.01-3% w/w, and water or DM water.
Accordingly, the present invention relates to a fungicidal composition comprising of: fungicides selected from acylamino acid class including Valifenalate and at least one strobilurin based fungicide and at least one azole-based fungicide. The fungicides selected from acylamino acid class is Valifenalate; strobilurin fungicide is selected from the group consisting of Azoxystrobin, Pyraclostrobin, Fluoxastrobin, Kresoxim-Methyl, Trifloxystrobin, Picoxystrobin, Mandestrobin, or Metominostrobin; and the azole-based fungicide is selected from the group consisting of Imazalil, Oxpoconazole Fumarate, Pefurazoate, Prochloraz, Triflumizole, Azaconazole, Bromuconazole, Bitertanol, Cyproconazole, Diclobutrazol, Difenoconazole, Diniconazole, Epoxiconazole, Etaconazole, Fenbuconazole, Fluquinconazole, Flusilazole, Flutriafol, Furconazole, Hexaconazole, Imibenconazole, Ipconazole, Metconazole, Myclobutanil, Penconazole, Propiconazole, Prothioconazole, Quinconazole, Simeconazole, Tebuconazole, Tetraconazole, Triadimefon, Triadimenol or Triticonazole
In further embodiment of the present invention, the Valifenalate is used in an amount of about 1-60%) weight of the total composition

In another embodiment of the present invention, the strobilurin based fungicide is used in a range of 1-40% weight of the total composition.
In further embodiment of the present invention, the strobilurin based fungicide is Trifloxystrobin.
In another embodiment of the present invention, the azole based fungicide is used in the range of 5-50% weight of the total composition.
In further embodiment of the present invention, the azole fungicide is Difenoconazole.
In another preferred embodiment, the present invention discloses a stable synergistic composition comprising 1-60 % w/w of valifenalate technical, 1-40 % w/w trifloxystrobin technical, 5-50 % w/w of Difenoconazole Technical, 0.5-10 % w/w dispersing agent, 0.5-10% wetting agent, 0.01-1 % w/w antifoaming agent, 0.1-10 % w/w anti freezing agetnt, 0.1-10 % w/w adjuants, 0.1-5 % w/w filler, 0.01-1 % w/w antibacterial/biocide, one or more of Polysaccharides, carboxymethyl cellulose, Bentonite Clay, Aluminum Magnesium Silicate in the range of 0.01-3% w/w, and water or DM water.
In preferred embodiment of the present invention, the composition comprises one or more agro chemically acceptable excipients.
In another embodiment of the present invention, the agrochemically acceptable excipients are selected from group comprising surfactants/dispersing agents, polymers, anti-freezing agent, wetting agents, anti-foaming agents, colorants, pigments, binder/structuring agents, biocides/anti-microbial agent, thickeners, emulsifiers, buffering agent, fillers, diluents/solvents, quick coating agents or sticking agents, preservatives, adjuvants and solvents or a combination thereof.
In another embodiment of the present invention, the thickeners are selected from the group comprising thickeners are selected from the group comprising silicates such metal silicates, aluminum magnesium silicate sodium carboxy methyl cellulose, methyl cellulose, ethyl cellulose, poly vinyl alcohol, sodium alginate, sodium poly acrylate, xanthan gum, welan gum, gum arabic, montmorillonite, lingo sulfonates, hydroxy methyl cellulose, dextrin, starch,

In another embodiment of the present invention, the anti-freezing agent is selected from the group comprising polyethylene glycols, methoxy polyethylene glycols, propylene glycol, polypropylene glycols, polybutylene glycols, Mono ethylene glycol, glycerin, ethylene glycol, Glycerin, Diethylene glycol or combinations thereof.
In another embodiment of the present invention, anti-foaming agent is selected from the group comprising silicone oils, or mineral oils, Fatty acid ester; potassium sorbate, and parahydroxy benzoates, silicon emulsion based anti-foam agents, Siloxane polyalkylene oxide, Polydimethyl Siloxane, tri siloxane ethoxylates or combinations thereof.
In another embodiment of the present invention, wetting agent is selected from the group comprising Tristyrylphenol ethoxylate nonionic emulsifier, Mixture of non-ionic surfactants & Alkoxylated Alcohol/Block copolymer, sodium lauryl sulphate; sodium dioctyl sulphosuccinate; Tristyrylphenol ethoxylate non-ionic emulsifier/ mixture of non-ionic surfactants and alkoxylated Alcohol/Block copolymer, ionic surfactant mixtures, anionic surfactants, alkyl phenol ethoxylates, aliphatic alcohol ethoxylates and the salts or combinations thereof.
In yet another embodiment of the present invention, the polymers are selected from the group comprising polyvinyl alcohols, polyvinylpyrrolidone, gel-forming carraagheenans, water-soluble gelatine and casein, superabsorbent polymers such polyacrylamides and polyacrylates based polymers, semi-synthetic or fully-synthetic peptide/protein-based superabsorbent polymers such as collagen-based synthetic polymers, elastin-like polypeptides, polyaspartic acid, polyaspartates, polyglutamic acid, polyglutamate, Semi-synthetic or fully-synthetic polysaccharide: carboxymethyl starch , sulfoethyl starch, carboxymethyl cellulose, sulfoethyl cellulos, hydroxypropyl cellulose, hydroxyethyl cellulose, methylcellulose, chitosan, acrylonitrile, acrylic acid, methacrylic acid, acrylamide, methacrylamide, 2-acrylamido-2-methyl- propanesulfonic acid (AMPS), vinyl sulfonic acid, ethyl acrylate, and potassium acrylate or combinations thereof.
In yet another embodiment of the present invention, the adjuvant is selected from the group comprising Silicone Ethoxylated Oil, Polyvinyl Pyrrolidon, Poly vinyl Alcohol,Blend of poly

comprising lactose, glucose, fructose, maltose, sucrose, in the anhydrous or hydrate forms, urea, water soluble or dispersible polymers, water soluble inorganic salts, Demineralized (DM) water, Vegetable Oil or combination thereof; and filer is selected from the group comprising Silicon Dioxide, China -Clay, Kaolin, Talc, starch or combination thereof.
In yet another embodiment of the present invention, the biocide/anti-microbial agent is selected from the group comprising Benzisothiazolin-3-one / Formaldehyde/Sodium benzoate/Sodium o-phenyl phenate, 5-chloro-2-methyl-4-isothiazolin-3-one & 2-methyl-4-isothiazolin-3-one or combination thereof.
In another embodiment of the present invention, the composition is in form of any one of as Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable Slurry (FS), Flowable Suspension(FS), Suspension Concentrate (SC), Suspension concentrate for direct application (SD), Suspo- emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Water soluble powder (SP), Water dispersible powder for slurry seed treatment (WS), Water dispersible granules (WDG), Wettable powders (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), granular (GR), oil in dispersion (OD), a mixed formulation of CS and SC (ZC) or a mixed formulation of CS and SE (ZE), a mixed formulation of CS and EW (ZW).
In another preffered embodiment of the present invention, the preferred composition is in form of Suspension Concentrate (SC), Water dispersible granules (WG), Suspo- emulsion (SE), or Suspo- emulsion (SE).
In yet another embodiment of the present invention, the agriculturally acceptable excipients of the formulation are selected from the group consisting of anti-freezing agent, dispersing agents, wetting agents, antifoaming agents, biocides, thickeners, fillers, antibacterial/biocides, Polysaccharides/carboxymethyl cellulose/Bentonite Clay/Aluminum Magnesium Silicate, and solvents. An adjuvant used in the present invention is any material that is added to an agrochemical formulation to enhance or modify the performance of the formulation. An adjuvants used in the present invention to make it a safer to ecological environmental, having low toxicity and having no phytotoxicity effects on any part of the plant.

In another embodiment of the present invention, the composition comprises of
a. valifenalate or its agro chemically acceptable salt in a range of 1-60% by weight of the
composition;
b. trifloxystrobin, or its agro chemically acceptable salt in a range of 1-40% by weight of
the composition;
c. difenoconazole or its agro chemically acceptable salt in a range of 5-50% by weight of
the composition; and
d. at least one or more agro chemically acceptable excipients.
In yet another embodiment of the present invention, the composition comprises of:
a. valifenalate or its agro chemically acceptable salt in a range of 10-50% by weight of
the composition;
b. trifloxystrobin, or its agro chemically acceptable salt in a range of 5-30% by weight of
the composition;
c. difenoconazole or its agro chemically acceptable salt in a range of 5-40% by weight of
the composition; and
d. at least one or more agro chemically acceptable excipients.
In another embodiment of the present invention, the composition comprises of
a. valifenalate or its agro chemically acceptable salt in a range of 10-30% by weight of
the composition;
b. trifloxystrobin, or its agro chemically acceptable salt in a range of 5-20% by weight of
the composition;
c. difenoconazole orits agro chemically acceptable salt in a range of 5-25% by weight of
the composition; and
d. at least one or more agro chemically acceptable excipients.
The compositions of the present invention acts synergistically to control pests in various crops.

In another embodiment of the present invention provides a method for controlling a plant disease caused by a fungal plant pathogens comprising applying to the plant or portion thereof or soil, a fungicidally effective amount of the fungicidal composition of the present invention.
In another embodiment of the present invention provides a method of improving vigour or yield of the plant comprising, treating a plant or portion thereof or soil, a fungicidally effective amount of the fungicidal composition.
In another embodiment of the present invention, the plant is selected from the group consisting of cereals, field crops, fruits, tree nuts, vegetables, turf grasses, potatoes, tomatoes, grapes and ornamentals.
The fungicide composition of the present invention is particularly useful as an agricultural and horticultural fungicide. Target diseases of the agricultural and horticultural fungicide, for example, include rice blast (Magnaporthe grisea), brown spot (Cochliobolus miyabeanus), sheath blight (Rhizoctonia solani), Bakanae disease (Gibberella fujikuroi), damping-off (Fusarium spp., Rhizopus spp., Pythium spp.) of rice; powdery mildew (Blumeria graminis), mottle leaf (Pyrenophora graminea), net blotch (Pyrenophora teres), Fusarium blight (Fusarium graminearum, Microdochium nivale), brown rust (Puccinia recondita), browning root rot (Pythium iwayamai), loose smut (Ustilago nuda), ergot (Clavicepspurpurea), eye spot (Pseudocercosporella herpotrichoides), scald (Rhynchosporium secalis), speckled leaf blotch (Septoria tritici), glume blotch (Septoria nodorum), Typhulabvown snow blight (Typhula incarnatd), Typhula black snow blight (Typhula ishikariensis), Sclerotinia snow blight (Sclerotinia borealis), pink snow mold (Microdochium nivale), damping-off (Gaeumannomyces graminis) of wheat and barley; brown spot (Cochliobolus heterostrophus), rust (Puccinia sorghi), northern leaf blight (Setosphaeria turcica), leaf spot (Physoderma maydis), smut (Ustilago maydis) of maize; rust (Puccinia melanocephala), smut (Ustilago scitaminea), top rot (Gibberella fujikuroi), leaf blight (Pseudocercospora taiwanensis) of sugar cane; black spot (Diaporthe citri), scab (Elsinoe fawcettii), gray mold (Botrytis cinerea) of citruses; blossom blight (Monilinia mali), apple canker (Valsa ceratosperma), powdery mildew (Podosphaera leucotricha), Alternaria blotch (Alternaria alternata apple pathotype), black spot (Venturia inaequalis), anthracnose (Colletotrichum acutatum, Glomerella cingulata) of apple; black spot (Venturia nashicola), Alternaria leaf spot (Alternaria alternata Japanese pear pathotype), chocolate spot (Gymnosporangium asiaticum),

late blight {Phytophthora cactorum), anthracnose (Colletotrichum acutatum, Glomerella
cingulata), ring spot (Botryosphaeria berengeriana), powdery mildew (Phyllactinia mali) of
pears; brown rot (Monilinia fructicold), black spot (Cladosporium
carpophilum), Phomopsis rot (Phomopsis sp.), bacterial shot hole (Leptosphaeria michotii) of
peach; downy mildew (Plasmopara viticola), bird's eye rot (Elsinoe ampelina), ripe rot
(Glomerella cingulata), powdery mildew (Uncinula necator), rust (Physppella ampelopsidis),
gray mold (Botrytis cinered) of grapes; anthracnose (Glomerella cingulata), anguler leaf spot
(Cercospora kaki), circular leaf spot (Mycosphaerella nawae), gray mold (Botrytis cinered),
powdery mildew (Phyllactinia kakicola), late blight (Phytophthora citrophthora) of
persimmon; powdery mildew (Sphaerotheca fuliginea), late blight (Phytophthora cryptogea),
brown rot (Phytophthora capsici), Sclerotinia rot (Sclerotinia sclerotiorum), damping-off
(Pythium debaryanum), gummy stem blight (Didymella bryoniae), Fusarium wilt (Fusarium
oxysporum), Verticillium wilt (Verticillium dahliae), downy mildew (Pseudoperonospora
cubensis) of watermelon; powdery mildew (Erysiphe polygoni, Sphaerotheca cucurbitae), late
blight (Phytophthora melonis), Corynespora leaf spot (Corynespora
cassiicola), Sclerotinia rot (Sclerotinia sclerotiorum), anthracnose (Colletotrichum orbiculare), gummy stem blight (Didymella bryoniae), Fusarium wilt (Fusarium oxysporum), damping-off (Pythium cucurbitacearum), gray mold (Botrytis cinered), Verticillium wilt (Verticillium dahliae), downy mildew (Pseudoperonospora cubensis) of cucumber; powdery mildew (Sphaerothecafuliginea), late blight (Phytophthora nicotianae), Corynespora leaf spot (Corynespora cassiicola), Sclerotinia rot (Sclerotinia sclerotiorum), gummy stem blight (Didymella bryoniae), Fusarium wilt (Fusarium oxysporum), damping-off (Pythium spinosum), gray mold (Botrytis cinered), Verticillium wilt (Verticillium dahliae), downy mildew (Pseudoperonospora cubensis) of melon; powdery mildew (Sphaerotheca cucurbitae), late blight (Phytophthora capsici), Sclerotinia rot (Sclerotinia sclerotiorum), gummy stem blight (Didymella bryoniae), downy mildew (Pseudoperonospora cubensis) of pumpkin; Fusarium wilt (Fusarium oxysporum), gray mold (Botrytis cinered), Sclerotinia rot (Sclerotinia sclerotiorum), southern blight (Sclerotium rolfsii), late blight (Phytophthora infestans), brown rot (Phytophthora capsici), brown spot (Phomopsis vexans), powdery mildew (Erysiphe cichoracerum, Oidiopsis sicula) of eggplant; late blight (Phytohthora infestans), early blight (Alternaria solani), black mole disease (Thanatephorus cucumeris), scab (Streptomyces spp.), bacterial soft rot (Pectobacterium carotovorum), powdery scab (Spongospora subterranean) of potato; late blight (Phytophthora infestans), fruit rot (Phytophthora capsici), early blight (Alternaria solani), leaf mold (Fulvia fulva), powdery

mildew (Oidium lycopersici), brown rot (Phytophthora nicotianae), anthracnose (Colletotrichum gloeosporioides), damping-off (Pythium vexans, Rhizoctonia solani) of tomato; late blight {Phytophthora capsici), powdery mildew (Oidiopsis sicula), seedling damping-off (Rhizoctonia solani), leaf spot (Cercospora capsici) of sweet pepper; late blight (Phytophthora sp.), downy mildew (Peronospora effusa), damping-off (Pythium aphanidermatum), Fusarium wilt (Fusarium oxysporum), stock rot (Rhizoctonia solani), root rot (Aphanomyces cochlioides) of spinach; white late blight (Phytophthora porri), late blight (Phytophthora nicotianae), southern blight (Sclerotium rolfsii), damping-off (Rhizoctonia solani), Alternaria leaf spot (Alternariaporri), soft rot (Pectobacterium carotovorum), downy mildew (Peronospora destructor), rust (Puccinia allii) of green onions; Fusarium basal rot (Fusarium oxysporum), small rot (Ciborinia alii), gray mold (Botrytis cinerea), gray rot (Botrytis allii) of onion; downy mildew (Peronospora parasitica), clubroot (Plasmodiophora brassicae), white rust (Albugo macrospora), Alternaria leaf spot (Alternaria brassicae), white spot (Cercosporella brassicae), Sclerotinia rot (Sclerotinia sclerotiorum), verticillium wilt (Verticillium dahliae), bottom rot (Rhizoctonia solani), foot rot (Aphanomyces raphani), Pythium rot (Pythium ultimum) of cruciferous vegetables; Sclerotinia rot (Sclerotinia sclerotiorum), rust (Phakopsora pachyrhizi), southern blight (Sclerotium rolfsii), damping-off (Fusarium oxysporum), anthracnose (Colletotrichum truncatum, C. trifolii, Glomerella glycines, Gloeosporium sp.), gray mold (Botrytis cinerea) of pulses; downy mildew (Peronospora manshurica), stem blight (Phytophthora sojae), purple speck (Cercospora kikuchii), Sphaceloma scad (Elsinoe glycines), pod and stem blight (Diaporthe phaseolorum) of soybean; leaf spot (Mycosphaerella personata), brown leaf spot (Mycosphaerella arachidis) of peanuts; powdery mildew (Sphaerotheca aphanis), Fusarium wilt (Fusarium oxysporum), Verticillium wilt (Verticillium dahliae), late blight (Phytophthora cactorum), Alternaria leaf spot (Alternaria alternata), leaf spot (Mycosphaerella fragariae), anthracnose (Colletotrichum acutatum, Glomerella cingulata), gray mold (Botrytis cinerea), bud blight (Rhizoctonia solani) of strawberry; stem rot (Sclerotinia sclerotiorum), bottom rot (Rhizoctonia solani), damping-off (Pythium sp.), root rot (Fusarium oxysporum), gray mold (Botrytis cinerea), downy mildew (Bremia lactucae) of lettuce; Fusarium wilt (Fusarium oxysporum), powdery mildew (Erysiphe heraclei), black leaf blight (Alternaria dauci), stain rot (Pythium sulcatum), root rot (Rhizoctonia solani), leaf spot (Cercospora carotae) of carrot; net blister blight (Exobasidium reticulatum), white scab (Elsinoe leucospila), brown round spot (Pseudocercospora ocellata, Cerocospora chaae), blister blight (Exobasidium vexans), ring spot (Pestaltiopsis longiseta), anthracnose (Discula

theae-sinensis) of tea; brown spot (Alternaria alternata), powdery mildew (Erysiphe cichoracearum), anthracnose (Colletotrichum cichoracearum) of tabacco; Cercospora leaf spot (Cercospora beticola) of sugar beet; black spot (Diplocarpon rosae), powdery mildew (Sphaerotheca pannosa), downy mildew (Peronospora sparsa), late blight (Phytophthora megasperma) of rose; powdery mildew (Erysiphe cichoracearum), late blight (Phytophthora cryptogea), Sclerotinia rot (Sclerotinia sclerotiorum), Alternaria leaf spot (Alternaria helianthi), downy mildew (Plasmopara halstedii), brown spot (Septoria helianthi) of sunflower; Fairy ring disease (Bovista dermoxantha, Lycoperdon pusillum, Conocybe lactea, Vascellum curtisii), pseudo-leaf rot (Ceratobasidium spp.), Curvularia leaf blight (Curvularia geniculata), leaf rot (Rhizoctonia solani), pink snow mold (Microdochium nivale), Pythium disease (Pythium spp.), rust (Puccinia spp.), dollar spot (Sclerotinia homoeocarpa), Pythium shoot blight (Pythium aphanidermatum), browning root rot (Pythium iwayamai), Typhula snow blight (Typhula incarnata, T. isikariensis), anthracnose (Colletotrichum sp.) of turf grass, and the like.
The ingredient (a), (b) and (c) constituting the fungicide composition of the present invention may be mixed with various adjuvants in the same manner as conventional agricultural chemicals, and used in the form of a formulation such as a dust, granules, water-dispersible granules, a wettable powder, a water-based suspension concentrate, an oil-based suspension concentrate, water-soluble granules, an emulsifiable concentrate, a soluble concentrate, a paste, an aerosol, or an ultralow-volume formulation. However, so long as it is suitable for the purpose of the present invention, it may be formulated into any type of formulation which is commonly used in this field. Such adjuvants to be used in the formulation include solid carriers such as diatomaceous earth, slaked lime, calcium carbonate, talc, white carbon, kaolin, bentonite, a mixture of kaolinite and sericite, clay, sodium carbonate, sodium bicarbonate, mirabilite, zeolite, and starch; solvents such as water, toluene, xylene, solvent naphtha, dioxane, acetone, isophorone, methyl isobutyl ketone, chlorobenzene, cyclohexane, dimethyl sulfoxide, dimethyl formamide, dimethyl acetamide, N-methyl-2-pyrrolidone, and alcohols; anionic surfactants and spreaders such as a salt of fatty acid, a benzoate salt, an alkylsulfosuccinate salt, a dialkyl sulfosuccinate salt, a polycarboxylate salt, a salt of alkylsulfuric acid ester, an alkyl sulfate salt, an alkylaryl sulfate salt, an alkyl diglycol ether sulfate salt, a salt of alcohol sulfuric acid ester, an alkyl sulfonate salt, an alkylaryl sulfonate salt, an aryl sulfonate salt, a lignin sulfonate salt, an alkyldiphenyl ether disulfonate salt, a polystyrene sulfonate salt, a salt of alkyl phosphoric acid ester, an alkylaryl phosphate salt, a

styrylaryl phosphate salt, a salt of polyoxyethylene alkyl ether sulfuric acid ester, a polyoxyethylene alkylaryl ether sulfate salt, a salt of polyoxyethylene alkylaryl ether sulfuric acid ester, a polyoxyethylene alkyl ether phosphate salt, a salt of polyoxyethylene alkylaryl phosphoric acid ester, and a salt of a condensate of naphthalene sulfonate-formalin; nonionic surfactants and spreaders such as a sorbitan fatty acid ester, a glycerin fatty acid ester, a fatty acid polyglyceride, a fatty acid alcohol polyglycol ether, acetylene glycol, acetylene alcohol, an oxyalkylene block polymer, a polyoxyethylene alkyl ether, a polyoxyethylene alkylaryl ether, a polyoxyethylene styrylaryl ether, a polyoxyethylene glycol alkyl ether, a polyoxyethylene fatty acid ester, a polyoxyethylene sorbitan fatty acid ester, a polyoxyethylene glycerin fatty acid ester, a polyoxyethylene hydrogenated castor oil, and a polyoxypropylene fatty acid ester; vegetable and mineral oils such as olive oil, kapok oil, castor oil, palm oil, camellia oil, coconut oil, sesame oil, corn oil, rice bran oil, peanut oil, cottonseed oil, soybean oil, rapeseed oil, linseed oil, tung oil, and liquid paraffin; and the like. Such adjuvants may be selected for use among those known in this field, so long as such selection does not depart from the purpose of the present invention. Further, it is possible to use commonly employed various adjuvants such as a filler, a thickener, an anti-settling agent, an anti-freezing agent, a dispersion stabilizer, a phytotoxicity reducing agent, an anti-mold agent, etc. In actual use of such a formulation, it may be used as it is, or after it is diluted with a diluting agent such as water to a predetermined concentration and, as a case requires, various spreaders are added.
The composition of the present invention comprising Valifenalate, strobilurin fungicide, and azole fungicide is stable, effective and synergistic. The following illustrations of examples are intended to illustrate a stable synergistic fungicidal composition, but in no way limit the scope of the present invention.
Example 1:
A stable Suspension Concentrate (SC) of Valifenalate, Trifloxystrobin, and Difenoconazole according to the present invention was prepared as follows:

Recipe wt%
Valifenalate Technical 20.00
Trifloxystrobin Technical 10.00
Difenoconazole Technical 10.00
Dispersing Agent- Tristyrylphenol Ethoxylate Amine 3.00

salt of phosphate
Wetting Agent- Block copolymer 2.00
Antifoaming Agent-Siloxane polyalkyleneoxide 0.20
Antifreezing Agent- Propylene Glycol 5%
Adjuants-Silicone ethoxylated Oil 1%
Filler- China -Clay 1%
Anti-bacterial -Benzisothiazolin-3-one 0.10
Polysaccharides 0.10
DM water Q.stomake 100
TOTAL 100.00
Process: Required quantity of water, biocide, and defoamer followed by addition of gum powder are homogenized with stirring to obtain a gum solution (Gum Solution should be made 12-18 hour prior to use). Required quantity of DM water, wetting agent, dispersing agent & suspending agents, colourant/dye was added into the charged vessel followed by homogenization for a period of ranging between 45 - 60 minutes using high shear homogeniser to obtain a homogenized slurry. Technical and other remaining adjuvants excluding 'antifreeze & thickeners' were added into the homogenized slurry to obtain a uniform slurry. Half of the quantity of required antifoam agent was added into the slurry. The uniform slurry mixture was then passed through appropriate particle size reduction equipment (Dyno-Mill) until the granule material of the desired particle size was achieved. Remaining half of the quantity of required antifoam agent along with the antifreeze agent was added to the granule material as obtained. Gum solution as obtained in the first step was then added to obtain the Suspension Concentrate formulation.
Exemplary compositions of Suspension concentrate are shown in below table:

SC (Suspension Concentrate)
Sr. No. Component Composition

1 2 3 4 5
1 Valifenalate Technical 50 2 10 3 14
2 Trifloxystrobin Technical 1 40 30 2 20
3 Difenoconazole Technical 5 6 5 50 15
4 Acrylic graft Copolymer 3.00 3.00 3.00 3.00 3.00

5 Naphthalene sulfonic acid,sodium salt condensate with formaldehyde 4.00 4.00 4.00 4.00 4.00
6 Ethoxylated Silicone oil 1.00 1.00 1.00 1.00 1.00
7 Silicone Antifoam 0.50 0.50 0.50 0.50 0.50
8 Benzisothiazoline 0.10 0.10 0.10 0.10 0.10
9 Glycol 5.00 5.00 5.00 5.00 5.00
10 Polysaccharides 0.10 0.10 0.10 0.10 0.10
11 DM water QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100
Example 2:
A stable Water Dispersible Granule (WG) formulation of Valifenalate, Trifloxystrobin, and Difenoconazole according to the present invention was prepared as follows:

S.No. Composition wt%
1 Valifenalate Technical 50.00
2 Trifloxystrobin Technical 15.00
3 Difenoconazole Technical 10.00
4 Dispersing agent-Sodium Polycarboxylate 3.00
5 Wetting agent-Sodium Lauryl Sulfate 4.00
6 Sodium alkyl naphthalene sulfonate blend 2.00
7 Adjuants-Polyvinyl pyrolliddone 2.00
8 Antifoam-Polydimethyl Siloxane 0.50
9 Filler - China Clay QSto Make 100
TOTAL 100.00
Process: Required quantity of filler, wetting agent, dispersing agent, suspending agent, and technical was mixed, in a premixing blender for homogenization for a period of 30 minutes to obtain a pre-blended material. The pre-blended material as obtained in the first step was blended through Jet mill/ air classifier mills followed by blending in post blender for a period of-1.5 hour to obtain a homogeneous mixture. Required quantity of water (qs) was then added

to make a dough. The dough was then passed through the extruder to obtain granules of required size. Wet granules as obtained were passed through the fluidised bed drier followed by grading using vibrating screens to obtain the wettable granules.
Other exemplary water dispersible granules (WG) compositions:

WG (Water Dispersible Granule)
Sr.
No. Component Composition

1 2 3 4 5
1 Valifenalate Technical 60 1 3 20 30
2 Trifloxystrobin Technical 1 40 5 25 20
3 Difenoconazole Technical 10 8 50 30 15
4 Sodium Polycarboxylate 8.00 11.00 10.00 1.00 10.00
5 Sodium Lauryl Sulfate 6.00 7.00 4.00 5.00 4.00
6 Sodium ligno sulfonate 2.00 1.00 1.00 1.00 1.00
7 Sodium alkylnaphthalenesulfonate, formaldehyde condensate 0.50 0.50 0.50 0.50 0.50
8 Silicone based antifoam 0.10 0.10 0.10 0.10 0.10
9 Ethoxylated Silicone oil 0.10 0.10 0.10 0.10 0.10
10 Precipitated Silica 0.10 0.10 0.10 0.10 0.10
11 China Clay QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100
Example 3:
A stable Wettable Powder (WP) of Valifenalate, Trifloxystrobin, and Difenoconazole according to the present invention was prepared as follows:

S.No. Composition wt%
1 Valifenalate Technical 50.00
2 Trifloxystrobin Technical 30.00

3 Difenoconazole Technical 5.00
4 Dispersing Agent-Sodium ligno sulfonate 3.00
5 Wetting Agent-Sodium Lauryl Sulfate 4.00
6 Sodium alkyl naphthalene sulfonate blend 1.00
7 Adjuants-Polyvinyl pyrolliddone 1.00
8 Antifoam-Polydimethyl siloxane 0.10
9 Filler-China Clay TOTAL 5.00

100.00
Process: Required quantity of filler, wetting agent, dispersing agent, suspending agent, and technical were charged in premixing blender for homogenization for a period of 30 minutes to obtain a pre-blended material. The pre-blended material was grinded through Jet mill/ air classifier mills followed by blending in post blender for a period of -1.5 hr to obtain a homogeneous material. The homogenous material as obtained was unloaded and analysed.
Few exemplary wettable powder composition:

WP (Wettable Powder)
Sr.
No. Component Composition

1 2 3 4 5
1 Valifenalate Technical 60 1 3 20 30
2 Trifloxystrobin Technical 1 40 5 25 20
3 Difenoconazole Technical 10 8 50 30 15
4 Sodium Polycarboxylate 8.00 8.00 8.00 8.00 8.00
5 Sodium Lauryl Sulfate 5.00 5.00 5.00 5.00 5.00
6 Blend of poly terpene resin 1.00 1.00 1.00 1.00 1.00
7 Sodium alkylnaphthalenesulfonate, formaldehyde condensate 0.50 0.50 0.50 0.50 0.50
8 Silicone based antifoam 0.10 0.10 0.10 0.10 0.10
9 Starch 5.00 5.00 5.00 5.00 5.00
10 Precipitated Silica 0.10 0.10 0.10 0.10 0.10
11 China Clay QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100

Example 4:
A stable Flowable concentrate for seed treatment (FS) of Valifenalate, Trifloxystrobin, and Difenoconazole according to the present invention was prepared as follows:

S.No. Recipe wt%
1 Valifenalate Technical 15.00
2 Trifloxystrobin Technical 10.00
3 Difenoconazole Technical 20.00
4 Dispersing Agent- Tristyrylphenol Ethoxylate Amine salt of phosphate 3.00
5 Wetting Agent-Block copolymer 2.00
6 Adjuants-Silicone ethoxylated Oil 1.00
7 Antifoaming Agent - Siloxane polylkyleneoxide 0.20
8 Anti-bacterial - Benzisothiazolin-3-one 0.10
9 Antifreezing Agent - Propylene Glycol 5.00
10 Filler - China Clay 3.00
11 Polysaccharides 0.10
12 DM water Q.stomake 100
TOTAL 100.00
Process: Required quantity of water, biocide, and defoamer followed by addition of gum powder are homogenized with stirring to obtain a gum solution (Gum Solution should be made 12-18 hour prior to use). Required quantity of DM water, wetting agent, dispersing agent & suspending agents, colourant/dye was added into the charged vessel followed by homogenization for a period of ranging between 45 - 60 minutes using high shear homogeniser to obtain a homogenized slurry. Technical and other remaining adjuvants excluding 'antifreeze & thickeners' were added into the homogenized slurry to obtain a uniform slurry. Half of the quantity of required antifoam agent was added into the slurry. The uniform slurry mixture was then passed through appropriate particle size reduction equipment (Dyno-Mill) until the granule material of the desired particle size was achieved. Remaining half of the quantity of required antifoam agent along with the antifreeze agent was added to the granule material as obtained. Gum solution as obtained in the first step was then added to obtain Flowable concentrate formulation.

Few exemplary flowable slurry composition

FS (Flowable Slurry)
Sr. No. Component Composition

1 2 3 4 5
1 Valifenalate Technical 50 2 10 3 14
2 Trifloxystrobin Technical 1 40 30 2 20
3 Difenoconazole Technical 5 6 5 50 15
4 Acrylic Copolymer 3.00 3.00 3.00 3.00 3.00
5 Naphthalene sulfonic acid,sodium salt condensate with formaldehyde 4.00 4.00 4.00 4.00 4.00
6 Ethoxylated Silicone oil 1.00 1.00 1.00 1.00 1.00
7 Silicone Antifoam 0.50 0.50 0.50 0.50 0.50
8 Benzisothiazoline 0.10 0.10 0.10 0.10 0.10
9 Glycol 5.00 5.00 5.00 5.00 5.00
10 Polysaccharides 0.10 0.10 0.10 0.10 0.10
11 DM water QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100
Example 5: A stable granule (GR) of Valifenalate, Trifloxystrobin, and Difenoconazole according to the present invention was prepared as follows.

S.No. Recipe wt%
1 Valifenalate Technical 2.00
2 Trifloxystrobin Technical 2.00
3 Difenoconazole Technical 5.00
4 Dispersing Agent- Calcium Ligno sulphate 3.00
5 Pigment Blue 0.20
6 Filler-China-Clay 5.00
7 Adjuants-Polyvinyl pyrolliddone 0.10

8 DM water 1
9 Sand Q.stomake 100
TOTAL 100.00
Process: Required quantities of filler, wetting agent, dispersing agent, suspending agent, and technicals into premixing blender for homogenization for 30 minutes. Pre-blended material is grinded through Jet mill/air classifier mills. Finely grinded material is blended in post blender, till it homogenises (approx. 1.5 hours). Homogenised material is analysed, and required quantity of sand added in granulator, followed by addition of DM water, sticking agent, and remaining material it become homogeneous. Further, finely grinded material is added to the mixture in the granulator, for 30 minutes to obtain final product.
Few exemplary granule compositions

GR (Granule)
Sr.
No. Component Composition

1 2 3 4 5
1 Valifenalate Technical 10 11 12 15 10
2 Trifloxystrobin Technical 3 5 5 5 5
3 Difenoconazole Technical 5 8 6 10 7
4 Sodium Polycarboxylate 3.00 3.00 3.00 3.00 3.00
5 Sodium Lauryl Sulfate 4.00 4.00 4.00 4.00 4.00
6 Pigment blue 0.10 0.10 0.10 0.10 0.10
7 Blend of poly terpene resin China Clay 0.50 0.50 0.50 0.50 0.50
9
5.00 5.00 5.00 5.00 5.00
10 DM water 1.00 2.00 1.00 2.00 1.00
11 Sand QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100
Example 6: A stable wettable powder (WP) of Valifenalate, Trifloxystrobin, and Difenconazole according to the present invention was prepared as follows.

S.No. Recipe wt%
1 Valifenalate Technical 50.00

2 Trifloxystrobin Technical 30.00
3 Difenoconazole Technical 5.00
4 Dispersing Agent-Sodium ligno sulfonate 3.00
5 Wetting Agent-Sodium Lauryl Sulfate 4.00
6 Dispersing Sodium alkyl naphthalene sulfonate blend 1.00
7 Adjuants-Polyvinyl pyrolliddone 1.00
8 Antifoam-Polydimethyl Siloxane 0.10
9 Filler -China Clay Q.stomake 100
TOTAL 100.00
Process: Required quantities of filler, wetting agent, dispersing agent, suspending agent and technical added to a premixing blender, and homogenised for 30 minutes. Grinding pre blended material through jet mill / or air classifier mill, followed by blending in post blender, and homogenised for 30 minutes, to obtain final product.
Other exemplary wettable powder compositions:

WP (Wettable Powder)
Sr. No. Component Composition

1 2 3 4 5
1 Valifenalate Technical 60 1 3 20 30
2 Trifloxystrobin Technical 1 40 5 25 20
3 Difenoconazole Technical 10 8 50 30 15
4 Sodium Polycarboxylate 8.00 8.00 8.00 8.00 8.00
5 Sodium Lauryl Sulfate 5.00 5.00 5.00 5.00 5.00
6 Blend of poly terpene resin 1.00 1.00 1.00 1.00 1.00
7 Sodium
alkylnaphthalenesulfonate, formaldehyde condensate 0.50 0.50 0.50 0.50 0.50
8 Silicone based antifoam 0.10 0.10 0.10 0.10 0.10
9 Starch 5.00 5.00 5.00 5.00 5.00
10 Precipitated Silica 0.10 0.10 0.10 0.10 0.10

QSto QSto QSto QSto QSto
11 China Clay Make Make Make Make Make
100 100 100 100 100
Example 7: A stable suspo emulsion (SE) formulation of Valifenalate, Trifloxystrobin, and Difenoconazole according to the present invention was prepared as follows.

S.No. Composition wt%
1 Valifenalate Technical 1.00
2 Trifloxystrobin Technical 40.00
3 Difenoconazole Technical 5.00
4 Sodium Polycarboxylate 7.00
5 Sodium Lauryl Sulfate 3.00
6 Sodium alkyl naphthalene sulfonate blend 2.00
7 Sodium alkylnaphthalenesulfonate, formaldehyde condensate 2.00
8 Silicone based antifoam 0.50
9 Starch 5.00
10 Lactose anhydrous 34.50
TOTAL 100.00
Process: Required amount of water, biocide, and defoamer homogenised, followed by slow addition of gum powder (prepared 12-18 hours prior to use) and stirred till complete dissolution. Followed by addition of water, wetting agent, dispersing agent, suspending agents, colourant/dye and homogenised for 45-60 minutes using high shear homogeniser. Technical and other remaining adjuvants (excluding antifreeze and thickeners) added and homogenises to obtain slurry. Half the amount of required antifoam was added and material grinded upto three cycles in Dyno mill. Remaining amount of antifoam and antifreeze added before in process analysis. Finally, gum solution added to obtain final product.
Other exemplary suspo emulsion compositions:

SE (Suspo Emulsion)
Sr.
No. Component Composition

1 2 3 4 5

1 Valifenalate Technical 50 2 10 3 14
2 Trifloxystrobin Technical 1 40 30 2 20
3 Difenoconazole Technical 5 6 5 50 15
4 Acrylic Copolymer 3.00 3.00 3.00 3.00 3.00
5 Naphthalene sulfonic acid,sodium salt condensate with formaldehyde 4.00 4.00 4.00 4.00 4.00
6 Ethoxylated Silicone oil 1.00 1.00 1.00 1.00 1.00
7 Silicone Antifoam 0.50 0.50 0.50 0.50 0.50
8 Benzisothiazoline 0.10 0.10 0.10 0.10 0.10
9 Glycol 5.00 5.00 5.00 5.00 5.00
10 1-Octanol 30.00 25.00 20.00 30.00 15.00
11 Polysaccharides 0.10 0.10 0.10 0.10 0.10
12 DM water QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100
Example 8: A stable oil dispersion (OD) formulation of Valifenalate, Trifloxystrobin, and Difenoconazole according to the present invention was prepared as follows.

S.No. Composition wt%
1 Valifenalate Technical 5.00
2 Trifloxystrobin Technical 25.00
3 Difenoconazole Technical 5.00
4 Dispersing Agent- Tristyrylphenol Ethoxylate Amine salt of phosphate 3.00
5 Dispersing Agent Ethoxylated oleyl cetyl alcohol 3.00
6 Sticking Agent-Polyvinylpyrrolidone 1.00
7 Emulsifier-Calcium alkyl benzen sulfonate 5.00
8 Emulsifier-Castor oil ethoxylates 4
9 Antifoam-Polydimethyl Siloxane 0.00
10 Antifreezing Agent- Propylene Glycol 0.00
11 Vegetable Oil-Soyabean Oil Q.s to
make
100

TOTAL

100.00

Process: Required quantity of vegetable oil, polyvinyl pyrrollidone, mixed for 30 minutes using high shear homogeniser. Further, required amount of wetting agent, dispersing agent, suspending agents, and homogenised for 45-60 minutes in high shear homogeniser. Followed by addition of technical and homogenisation of 30 minutes more. Homogenised material passed through horizontal bead mill to get required particle size.
Exemplary oil dispersion composition:

OD (Oil Dispersion)
Sr. No. Component Composition

1 2 3 4 5
1 Valifenalate Technical 20 2 10 3 14
2 Trifloxystrobin Technical 1 30 15 2 10
3 Difenoconazole Technical 5 6 5 25 12
4 Ethoxylated oleyl cetyl alcohol 3.00 3.00 3.00 3.00 3.00
5 Polyalkelene glycol ether 4.00 4.00 4.00 4.00 4.00
6 Polyvinyl pyrrolidone 1.00 1.00 1.00 1.00 1.00
7 Calcium alkyl benzen sulfonate 3.00 3.00 3.00 3.00 3.00
8 Castor oil ethoxylates 2.00 2.00 2.00 2.00 2.00
9 Silicone based antifoam 0.10 0.10 0.10 0.10 0.10
10 Propylene glycol 5.00 5.00 5.00 5.00 5.00
11 Soyabean Oil QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100
Example 9: A stable Wettable Powder (WP) of Valifenalate, Trifloxystrobin, and Difenoconazole according to the present invention was prepared as follows. The process of example 3 was followed to prepare the composition.

S.No. Composition wt%
1 Valifenalate Technical 1.00
2 Trifloxystrobin Technical 40.00
3 Difenoconazole Technical 5.00
4 Sodium Polycarboxylate 7.00
5 Sodium Lauryl Sulfate 3.00
6 Sodium alkyl naphthalene sulfonate blend 2.00
7 Sodium alkylnaphthalenesulfonate, formaldehyde condensate 2.00
8 Silicone based antifoam 0.50
9 Starch 5.00
10 Lactose anhydrous 34.50
TOTAL 100.00
Example 10: A stable Wettable Powder (WP) of Valifenalate, Trifloxystrobin, and Difenoconazole according to the present invention was prepared as follows. The process of example 3 was followed to prepare the composition.

S.No. Composition wt%
1 Valifenalate Technical 1.00
2 Trifloxystrobin Technical 1.00
3 Difenoconazole Technical 50.00
4 Sodium Polycarboxylate 7.00
5 Sodium Lauryl Sulfate 3.00
6 Sodium alkyl naphthalene sulfonate blend 2.00
7 Sodium alkylnaphthalenesulfonate, formaldehyde condensate 2.00
8 Silicone based antifoam 0.50
9 Starch 5.00
10 Lactose anhydrous 28.50
TOTAL 100.00
Example 11: A stable Flowable concentrate for seed treatment (FS) of Valifenalate, Trifloxystrobin, and Difenoconazole according to the present invention was prepared as follows. The process of example 4 was followed to prepare the composition.

S.No. Recipe wt%
1 Valifenalate Technical 1.00
2 Trifloxystrobin Technical 40.00
3 Difenoconazole Technical 5.00
4 Acrylic copolymer 3.00
5 Block copolymer 4.00
6 Naphthalene sulfonic acid, sodium salt condensate with formaldehyde 1.00
7 Silicone antifoam 0.50
8 Benzisothiazoline 0.10
9 Glycol 5.00
10 Bentonite Clay 3.00
11 DM water 37.40
TOTAL 100.00
Example 12: A stable Flowable concentrate for seed treatment (FS) of Valifenalate, Trifloxystrobin and Difenoconazole according to the present invention was prepared as follows. The process of example 4 was followed to prepare the composition.

S.No. Recipe wt%
1 Valifenalate Technical 1.00
2 Trifloxystrobin Technical 1.00
3 Difenoconazole Technical 50.00
4 Acrylic copolymer 3.00
5 Block copolymer 4.00
6 Naphthalene sulfonic acid, sodium salt condensate with formaldehyde 1.00
7 Silicone antifoam 0.50
8 Benzisothiazoline 0.10
9 Glycol 5.00
10 Bentonite Clay 3.00
11 DM water 31.40
TOTAL 100.00

In an exemplary embodiment, preferred combinations are tabulated herein below. These combinations are only the preferred embodiments of the invention and are not to be construed at limiting.

Sr. no Strobilurin fungicide Azole fungicide
1. Valifenalate Azoxystrobin Imazalil
2. Valifenalate Azoxystrobin Oxpoconazole Fumarate
3. Valifenalate Azoxystrobin Pefurazoate
4. Valifenalate Azoxystrobin Prochloraz
5. Valifenalate Azoxystrobin Triflumizole
6. Valifenalate Azoxystrobin Azaconazole
7. Valifenalate Azoxystrobin Bromuconazole
8. Valifenalate Azoxystrobin Bitertanol
9. Valifenalate Azoxystrobin Cyproconazole
10. Valifenalate Azoxystrobin Diclobutrazol
11. Valifenalate Azoxystrobin Difenoconazole
12. Valifenalate Azoxystrobin Diniconazole
13. Valifenalate Azoxystrobin Epoxiconazole
14. Valifenalate Azoxystrobin Etaconazole
15. Valifenalate Azoxystrobin Fenbuconazole
16. Valifenalate Azoxystrobin Fluquinconazole
17. Valifenalate Azoxystrobin Flusilazole
18. Valifenalate Azoxystrobin Flutriafol
19. Valifenalate Azoxystrobin Furconazole
20. Valifenalate Azoxystrobin Hexaconazole
21. Valifenalate Azoxystrobin Imibenconazole
22. Valifenalate Azoxystrobin Ipconazole
23. Valifenalate Azoxystrobin Metconazole
24. Valifenalate Azoxystrobin Myclobutanil
25. Valifenalate Azoxystrobin Penconazole
26. Valifenalate Azoxystrobin Propiconazole
27. Valifenalate Azoxystrobin Prothioconazole
28. Valifenalate Azoxystrobin Quinconazole

29. Valifenalate Azoxystrobin Simeconazole
30. Valifenalate Azoxystrobin Tebuconazole
31. Valifenalate Azoxystrobin Tetraconazole
32. Valifenalate Azoxystrobin Triadimefon
33. Valifenalate Azoxystrobin Triadimenol
34. Valifenalate Azoxystrobin Triticonazole
35. Valifenalate Pyraclostrobin Imazalil
36. Valifenalate Pyraclostrobin Oxpoconazole Fumarate
37. Valifenalate Pyraclostrobin Pefurazoate
38. Valifenalate Pyraclostrobin Prochloraz
39. Valifenalate Pyraclostrobin Triflumizole
40. Valifenalate Pyraclostrobin Azaconazole
41. Valifenalate Pyraclostrobin Bromuconazole
42. Valifenalate Pyraclostrobin Bitertanol
43. Valifenalate Pyraclostrobin Cyproconazole
44. Valifenalate Pyraclostrobin Diclobutrazol
45. Valifenalate Pyraclostrobin Difenoconazole
46. Valifenalate Pyraclostrobin Diniconazole
47. Valifenalate Pyraclostrobin Epoxiconazole
48. Valifenalate Pyraclostrobin Etaconazole
49. Valifenalate Pyraclostrobin Fenbuconazole
50. Valifenalate Pyraclostrobin Fluquinconazole
51. Valifenalate Pyraclostrobin Flusilazole
52. Valifenalate Pyraclostrobin Flutriafol
53. Valifenalate Pyraclostrobin Furconazole
54. Valifenalate Pyraclostrobin Hexaconazole
55. Valifenalate Pyraclostrobin Imibenconazole
56. Valifenalate Pyraclostrobin Ipconazole
57. Valifenalate Pyraclostrobin Metconazole
58. Valifenalate Pyraclostrobin Myclobutanil
59. Valifenalate Pyraclostrobin Penconazole
60. Valifenalate Pyraclostrobin Propiconazole

61. Valifenalate Pyraclostrobin Prothioconazole
62. Valifenalate Pyraclostrobin Quinconazole
63. Valifenalate Pyraclostrobin Simeconazole
64. Valifenalate Pyraclostrobin Tebuconazole
65. Valifenalate Pyraclostrobin Tetraconazole
66. Valifenalate Pyraclostrobin Triadimefon
67. Valifenalate Pyraclostrobin Triadimenol
68. Valifenalate Pyraclostrobin Triticonazole
69. Valifenalate Fluoxastrobin Imazalil
70. Valifenalate Fluoxastrobin Oxpoconazole Fumarate
71. Valifenalate Fluoxastrobin Pefurazoate
72. Valifenalate Fluoxastrobin Prochloraz
73. Valifenalate Fluoxastrobin Triflumizole
74. Valifenalate Fluoxastrobin Azaconazole
75. Valifenalate Fluoxastrobin Bromuconazole
76. Valifenalate Fluoxastrobin Bitertanol
77. Valifenalate Fluoxastrobin Cyproconazole
78. Valifenalate Fluoxastrobin Diclobutrazol
79. Valifenalate Fluoxastrobin Difenoconazole
80. Valifenalate Fluoxastrobin Diniconazole
81. Valifenalate Fluoxastrobin Epoxiconazole
82. Valifenalate Fluoxastrobin Etaconazole
83. Valifenalate Fluoxastrobin Fenbuconazole
84. Valifenalate Fluoxastrobin Fluquinconazole
85. Valifenalate Fluoxastrobin Flusilazole
86. Valifenalate Fluoxastrobin Flutriafol
87. Valifenalate Fluoxastrobin Furconazole
88. Valifenalate Fluoxastrobin Hexaconazole
89. Valifenalate Fluoxastrobin Imibenconazole
90. Valifenalate Fluoxastrobin Ipconazole
91. Valifenalate Fluoxastrobin Metconazole
92. Valifenalate Fluoxastrobin Myclobutanil

93. Valifenalate Fluoxastrobin Penconazole
94. Valifenalate Fluoxastrobin Propiconazole
95. Valifenalate Fluoxastrobin Prothioconazole
96. Valifenalate Fluoxastrobin Quinconazole
97. Valifenalate Fluoxastrobin Simeconazole
98. Valifenalate Fluoxastrobin Tebuconazole
99. Valifenalate Fluoxastrobin Tetraconazole
100. Valifenalate Fluoxastrobin Triadimefon
101. Valifenalate Fluoxastrobin Triadimenol
102. Valifenalate Fluoxastrobin Triticonazole
103. Valifenalate Kresoxim-methyl Imazalil
104. Valifenalate Kresoxim-methyl Oxpoconazole Fumarate
105. Valifenalate Kresoxim-methyl Pefurazoate
106. Valifenalate Kresoxim-methyl Prochloraz
107. Valifenalate Kresoxim-methyl Triflumizole
108. Valifenalate Kresoxim-methyl Azaconazole
109. Valifenalate Kresoxim-methyl Bromuconazole
110. Valifenalate Kresoxim-methyl Bitertanol
111. Valifenalate Kresoxim-methyl Cyproconazole
112. Valifenalate Kresoxim-methyl Diclobutrazol
113. Valifenalate Kresoxim-methyl Difenoconazole
114. Valifenalate Kresoxim-methyl Diniconazole
115. Valifenalate Kresoxim-methyl Epoxiconazole
116. Valifenalate Kresoxim-methyl Etaconazole
117. Valifenalate Kresoxim-methyl Fenbuconazole
118. Valifenalate Kresoxim-methyl Fluquinconazole
119. Valifenalate Kresoxim-methyl Flusilazole
120. Valifenalate Kresoxim-methyl Flutriafol
121. Valifenalate Kresoxim-methyl Furconazole
122. Valifenalate Kresoxim-methyl Hexaconazole
123. Valifenalate Kresoxim-methyl Imibenconazole
124. Valifenalate Kresoxim-methyl Ipconazole

125. Valifenalate Kresoxim-methyl Metconazole
126. Valifenalate Kresoxim-methyl Myclobutanil
127. Valifenalate Kresoxim-methyl Penconazole
128. Valifenalate Kresoxim-methyl Propiconazole
129. Valifenalate Kresoxim-methyl Prothioconazole
130. Valifenalate Kresoxim-methyl Quinconazole
131. Valifenalate Kresoxim-methyl Simeconazole
132. Valifenalate Kresoxim-methyl Tebuconazole
133. Valifenalate Kresoxim-methyl Tetraconazole
134. Valifenalate Kresoxim-methyl Triadimefon
135. Valifenalate Kresoxim-methyl Triadimenol
136. Valifenalate Kresoxim-methyl Triticonazole
137. Valifenalate Trifloxystrobin Imazalil
138. Valifenalate Trifloxystrobin Oxpoconazole Fumarate
139. Valifenalate Trifloxystrobin Pefurazoate
140. Valifenalate Trifloxystrobin Prochloraz
141. Valifenalate Trifloxystrobin Triflumizole
142. Valifenalate Trifloxystrobin Azaconazole
143. Valifenalate Trifloxystrobin Bromuconazole
144. Valifenalate Trifloxystrobin Bitertanol
145. Valifenalate Trifloxystrobin Cyproconazole
146. Valifenalate Trifloxystrobin Diclobutrazol
147. Valifenalate Trifloxystrobin Difenoconazole
148. Valifenalate Trifloxystrobin Diniconazole
149. Valifenalate Trifloxystrobin Epoxiconazole
150. Valifenalate Trifloxystrobin Etaconazole
151. Valifenalate Trifloxystrobin Fenbuconazole
152. Valifenalate Trifloxystrobin Fluquinconazole
153. Valifenalate Trifloxystrobin Flusilazole
154. Valifenalate Trifloxystrobin Flutriafol
155. Valifenalate Trifloxystrobin Furconazole
156. Valifenalate Trifloxystrobin Hexaconazole

157. Valifenalate Trifloxystrobin Imibenconazole
158. Valifenalate Trifloxystrobin Ipconazole
159. Valifenalate Trifloxystrobin Metconazole
160. Valifenalate Trifloxystrobin Myclobutanil
161. Valifenalate Trifloxystrobin Penconazole
162. Valifenalate Trifloxystrobin Propiconazole
163. Valifenalate Trifloxystrobin Prothioconazole
164. Valifenalate Trifloxystrobin Quinconazole
165. Valifenalate Trifloxystrobin Simeconazole
166. Valifenalate Trifloxystrobin Tebuconazole
167. Valifenalate Trifloxystrobin Tetraconazole
168. Valifenalate Trifloxystrobin Triadimefon
169. Valifenalate Trifloxystrobin Triadimenol
170. Valifenalate Trifloxystrobin Triticonazole
171. Valifenalate Picoxystrobin Imazalil
172. Valifenalate Picoxystrobin Oxpoconazole Fumarate
173. Valifenalate Picoxystrobin Pefurazoate
174. Valifenalate Picoxystrobin Prochloraz
175. Valifenalate Picoxystrobin Triflumizole
176. Valifenalate Picoxystrobin Azaconazole
177. Valifenalate Picoxystrobin Bromuconazole
178. Valifenalate Picoxystrobin Bitertanol
179. Valifenalate Picoxystrobin Cyproconazole
180. Valifenalate Picoxystrobin Diclobutrazol
181. Valifenalate Picoxystrobin Difenoconazole
182. Valifenalate Picoxystrobin Diniconazole
183. Valifenalate Picoxystrobin Epoxiconazole
184. Valifenalate Picoxystrobin Etaconazole
185. Valifenalate Picoxystrobin Fenbuconazole
186. Valifenalate Picoxystrobin Fluquinconazole
187. Valifenalate Picoxystrobin Flusilazole
188. Valifenalate Picoxystrobin Flutriafol

189. Valifenalate Picoxystrobin Furconazole
190. Valifenalate Picoxystrobin Hexaconazole
191. Valifenalate Picoxystrobin Imibenconazole
192. Valifenalate Picoxystrobin Ipconazole
193. Valifenalate Picoxystrobin Metconazole
194. Valifenalate Picoxystrobin Myclobutanil
195. Valifenalate Picoxystrobin Penconazole
196. Valifenalate Picoxystrobin Propiconazole
197. Valifenalate Picoxystrobin Prothioconazole
198. Valifenalate Picoxystrobin Quinconazole
199. Valifenalate Picoxystrobin Simeconazole
200. Valifenalate Picoxystrobin Tebuconazole
201. Valifenalate Picoxystrobin Tetraconazole
202. Valifenalate Picoxystrobin Triadimefon
203. Valifenalate Picoxystrobin Triadimenol
204. Valifenalate Picoxystrobin Triticonazole
205. Valifenalate Mandestrobin Imazalil
206. Valifenalate Mandestrobin Oxpoconazole Fumarate
207. Valifenalate Mandestrobin Pefurazoate
208. Valifenalate Mandestrobin Prochloraz
209. Valifenalate Mandestrobin Triflumizole
210. Valifenalate Mandestrobin Azaconazole
211. Valifenalate Mandestrobin Bromuconazole
212. Valifenalate Mandestrobin Bitertanol
213. Valifenalate Mandestrobin Cyproconazole
214. Valifenalate Mandestrobin Diclobutrazol
215. Valifenalate Mandestrobin Difenoconazole
216. Valifenalate Mandestrobin Diniconazole
217. Valifenalate Mandestrobin Epoxiconazole
218. Valifenalate Mandestrobin Etaconazole
219. Valifenalate Mandestrobin Fenbuconazole
220. Valifenalate Mandestrobin Fluquinconazole

221. Valifenalate Mandestrobin Flusilazole
222. Valifenalate Mandestrobin Flutriafol
223. Valifenalate Mandestrobin Furconazole
224. Valifenalate Mandestrobin Hexaconazole
225. Valifenalate Mandestrobin Imibenconazole
226. Valifenalate Mandestrobin Ipconazole
227. Valifenalate Mandestrobin Metconazole
228. Valifenalate Mandestrobin Myclobutanil
229. Valifenalate Mandestrobin Penconazole
230. Valifenalate Mandestrobin Propiconazole
231. Valifenalate Mandestrobin Prothioconazole
232. Valifenalate Mandestrobin Quinconazole
233. Valifenalate Mandestrobin Simeconazole
234. Valifenalate Mandestrobin Tebuconazole
235. Valifenalate Mandestrobin Tetraconazole
236. Valifenalate Mandestrobin Triadimefon
237. Valifenalate Mandestrobin Triadimenol
238. Valifenalate Mandestrobin Triticonazole
239. Valifenalate Metominostrobin Imazalil
240. Valifenalate Metominostrobin Oxpoconazole Fumarate
241. Valifenalate Metominostrobin Pefurazoate
242. Valifenalate Metominostrobin Prochloraz
243. Valifenalate Metominostrobin Triflumizole
244. Valifenalate Metominostrobin Azaconazole
245. Valifenalate Metominostrobin Bromuconazole
246. Valifenalate Metominostrobin Bitertanol
247. Valifenalate Metominostrobin Cyproconazole
248. Valifenalate Metominostrobin Diclobutrazol
249. Valifenalate Metominostrobin Difenoconazole
250. Valifenalate Metominostrobin Diniconazole
251. Valifenalate Metominostrobin Epoxiconazole
252. Valifenalate Metominostrobin Etaconazole

253. Valifenalate Metominostrobin Fenbuconazole
254. Valifenalate Metominostrobin Fluquinconazole
255. Valifenalate Metominostrobin Flusilazole
256. Valifenalate Metominostrobin Flutriafol
257. Valifenalate Metominostrobin Furconazole
258. Valifenalate Metominostrobin Hexaconazole
259. Valifenalate Metominostrobin Imibenconazole
260. Valifenalate Metominostrobin Ipconazole
261. Valifenalate Metominostrobin Metconazole
262. Valifenalate Metominostrobin Myclobutanil
263. Valifenalate Metominostrobin Penconazole
264. Valifenalate Metominostrobin Propiconazole
265. Valifenalate Metominostrobin Prothioconazole
266. Valifenalate Metominostrobin Quinconazole
267. Valifenalate Metominostrobin Simeconazole
268. Valifenalate Metominostrobin Tebuconazole
269. Valifenalate Metominostrobin Tetraconazole
270. Valifenalate Metominostrobin Triadimefon
271. Valifenalate Metominostrobin Triadimenol
272. Valifenalate Metominostrobin Triticonazole
Additionally, the fungicide composition of the present invention may be used in combination with other agricultural chemicals, for example, a fungicide, an insecticide, a miticide, a nematicide, a soil pesticide, an antivirus agent, an attractant, an herbicide, and a plant growth regulating agent. In this case, a further excellent effect may be exhibited.
Example 13
Evaluation of synergistic effect of the fungicidal composition of the present invention:
Evaluation of the synergistic effect of the composition comprising, Trifloxystrobin, Valifenalate and Difenoconazole prepared by the process described in the above examples.
Synergism can be calculated by using the Colby's method i.e. the expected (or predicted) response of the combination is calculated by taking the product of the observed response for

each individual component of the combination when applied alone divided by 100 and subtracting this value from the sum of observed response for each component when applied alone. Synergism of the combination is then determined by comparing the observed response of the combination to the expected (or predicted) response as calculated from the observed response of each individual component alone. If the observed response of the combination is greater than the expected (or predicted) response, then the combination is said to be synergistic and falls within the definition of synergistic effect. (Colby, S. R., Weeds, 1967(15), p. 20-22).
The synergistic action expected for a given combination of two active components can be
calculated as follows:
XY
E = (X + Y)-
100
The synergistic action expected for a given combination of three active components can be
calculated as follows:
(XY+YZ+XZ) (XYZ)
E = (X + Y + Z) - +
100 10000
Where:
E represents expected percentage of pesticidal control for the combination of the two or three
active ingredients at defined doses (for example equal to x, y and z, respectively).
X is the percentage of pesticidal control observed by the compound (Trifloxystrobin) at a defined dose (equal to x).
Y is the percentage of pesticidal control observed by the compound (Valifenalate) at a defined dose (equal to y).
Z is the percentage of pesticidal control observed by the compound (Difenoconazole) at a defined dose (equal to z).
When the percentage of pesticidal control observed for the combination is greater than the
expected percentage, there is a synergism effect.
Observed control (%)
Ratio =
Expected control (%)
Ratio of O/E > 1, synergism observed

The present invention is illustrated by way of examples, the examples are meant for illustrative purposes and should not be construed as limiting.
Evaluation of the present composition on tomato crop:
Field experiment for synergistic activity of Trifloxystrobin, Valifenalate and Difenoconazole for the control of early blight (caused by Alternaria solani) and late blight (caused by Phytophthora infestans) diseases in tomato crop were conducted at farmer field, Sonipat (Haryana).
To evaluate the synergistic effect, tomato (Variety: Pusa Rubi) crop was sown in 4 m x 5 m plots maintaining 30 cm x 30 cm plant distance with three replications and in Randomized Block Design (RBD) during July to October, 2021. The treatments were applied as foliar spray in the form of SC formulation @ 250 ml/ha on initiation of disease incidence and repeat applied at 15 days' interval. The active components were applied at 2-3 doses in different ternary and binary combinations. Solo components were also taken for comparison. The treatment details are as under:
Tl - Trifloxystrobin + Valifenalate + Difenoconazole (40%+l%+15%)
T2 - Trifloxystrobin + Valifenalate + Difenoconazole (l%+60%+5%)
T3 - Trifloxystrobin + Valifenalate + Difenoconazole (10%+l%+50%)
T4 - Trifloxystrobin + Valifenalate + Difenoconazole (10%+20%+15%)
T5 - Trifloxystrobin + Valifenalate + Difenoconazole (20%+30%+5%)
T6 - Trifloxystrobin + Valifenalate (40%+1%)
T7 - Trifloxystrobin + Valifenalate (l%+60%)
T8 - Trifloxystrobin + Valifenalate (10%+1%)
T9 - Trifloxystrobin + Valifenalate (10%+20%)
T10 - Trifloxystrobin + Valifenalate (20%+30%)
Til - Trifloxystrobin + Difenoconazole (40%+15%)
T12 - Trifloxystrobin + Difenoconazole (l%+5%)
T13 - Trifloxystrobin + Difenoconazole (10%+50%)
T14 - Trifloxystrobin + Difenoconazole (10%+15%)
T15 - Trifloxystrobin + Difenoconazole (20%+5%)
T16 - Valifenalate + Difenoconazole (1%+15%)
T17 - Valifenalate + Difenoconazole (60%+5%)

T18 - Valifenalate + Difenoconazole (l%+50%)
T19 - Valifenalate + Difenoconazole (20%+15%)
T20 - Valifenalate + Difenoconazole (30%+5%)
T21 - Trifloxystrobin (1%)
T22 - Trifloxystrobin (10%)
T23 - Trifloxystrobin (20%)
T24 - Trifloxystrobin (40%)
T25 - Valifenalate (1%)
T26 - Valifenalate (20%)
T27 - Valifenalate (30%)
T28 - Valifenalate (60%)
T29 - Difenoconazole (5%)
T30 - Difenoconazole (15%)
T31 - Difenoconazole (50%)
T32 - Untreated check
The treatments Tl to T31 were applied as foliar spray and in T32 control treatment only water was sprayed. The observations for early blight disease caused by Alternaria solani and late blight caused by Phytophthora infestans incidence were recorded before first spray and at 10 days after each spray by observing three leaves of randomly selected ten random plants per plot.
0-9 Scale for early blight disease
Score Symptoms
0 No symptom on the leaf
1 Small irregular brown spots covering 1% or less of the leaf area
_ Small, irregular, brown spots with concentric rings covering 1 - 10% of the leaf
area <- Lesions enlarging, irregular, brown with concentric rings, cover 11 - 25% of leaf
area 7 Lesions coalesce to form irregular, dark brown patches with concentric rings
covering 26 - 50% of leaf area. Lesions on stems and petioles Q Lesions coalesce to form irregular, dark brown patches with concentric rings
covering more than 51% of leaf area. Lesions on stems and petioles
0-9 Scale for late blight disease

Score Symptoms
0 No symptoms observed
1 Only a few plants affected here and there, upto 1 or 2 spots in 12 yards radius. Upto
10 spots per plant, or general light spotting
3 About 50 spots per plant, or upto 1 leaflet in 10 attacked
<- Nearly every leaflet with lesions, plants still retaining normal form; field may smell of blight but looks green, although every plant is affected
Every plant affected and about V2 of leaf area destroyed by blight; field looks green flecked with brown
About 3/4 of leaf area destroyed by blight; field looks either predominantly brown or 9 green. In some varieties the youngest leaves escape infection. Only few leaves left green but stem remain green. All leaves dead, stems dead or drying.
Based on the data recorded per cent disease index (PDI) was calculated. The per cent reduction in disease incidence was also calculated over control. Based on the per cent reduction in disease incidence the Expected control was calculated and compared with Observed control. The results of the trial have been presented here under in Table 1.
Table 1: Synergistic effect of the present invention (Trifloxystrobin + Valifenalate + Difenoconazole) against early blight and late blight diseases in tomato crop

s.
No. Treatment Formulation Observed per cent control Expected per cent control Colby Ratio

Early blight Late blight Early blight Late blight Early blight Late blight
Tl Trifloxystrobin + Valifenalate + Difenoconazole 40% + 1% + 15% 78.43 77.12 67.23 62.30 1.167 1.238
T2 Trifloxystrobin + Valifenalate + Difenoconazole 1% + 60% + 5% 81.37 71.19 66.13 55.44 1.230 1.284
T3 Trifloxystrobin + Valifenalate + Difenoconazole 10%+1% + 50% 79.41 81.36 60.24 62.36 1.318 1.305
T4 Trifloxystrobin + Valifenalate + Difenoconazole 10%+ 20% + 15% 78.43 78.81 58.45 57.15 1.342 1.379
T5 Trifloxystrobin + Valifenalate + Difenoconazole 20% + 30%
+ 5% 86.27 83.05 70.36 61.49 1.226 1.351
T6 Trifloxystrobin + Valifenalate 40% + 1% 52.94 45.76 47.98 41.32 1.103 1.107
T7 Trifloxystrobin + Valifenalate 1% + 60% 54.90 41.53 48.94 37.30 1.122 1.113

T8 Trifloxystrobin + Valifenalate 10% +1% 36.27 39.83 36.16 36.55 1.003 1.090
T9 Trifloxystrobin + Valifenalate 10%+ 20% 42.16 45.76 38.49 41.22 1.095 1.110
T10 Trifloxystrobin + Valifenalate 20% + 30% 55.88 46.61 51.06 41.86 1.095 1.114
Til Trifloxystrobin + Difenoconazole 40%+15% 45.10 43.22 43.54 38.85 1.036 1.112
T12 Trifloxystrobin + Difenoconazole 1% + 5% 29.41 32.20 27.40 29.64 1.073 1.087
T13 Trifloxystrobin + Difenoconazole 10%+ 50% 37.25 39.83 35.38 34.54 1.053 1.153
T14 Trifloxystrobin + Difenoconazole 10%+15% 33.33 37.29 30.71 33.88 1.085 1.101
T15 Trifloxystrobin + Difenoconazole 20% + 5% 34.31 36.44 33.53 36.17 1.024 1.008
T16 Valifenalate + Difenoconazole 1%+15% 31.37 37.29 29.85 32.45 1.051 1.149
T17 Valifenalate + Difenoconazole 60% + 5% 44.12 36.44 44.02 34.72 1.002 1.050
T18 Valifenalate + Difenoconazole 1% + 50% 39.22 38.14 34.58 33.14 1.134 1.151
T19 Valifenalate + Difenoconazole 20%+15% 33.33 38.98 32.42 37.43 1.028 1.041
T20 Valifenalate + Difenoconazole 30% + 5% 42.16 34.75 41.40 33.27 1.018 1.044
T21 Trifloxystrobin 1% 18.63 17.80 - - - -
T22 Trifloxystrobin 10% 20.59 21.19
T23 Trifloxystrobin 20% 25.49 25.42 - - - -
T24 Trifloxystrobin 40% 35.29 27.12
T25 Valifenalate 1% 19.61 19.49
T26 Valifenalate 20% 22.55 25.42 - - - -
T27 Valifenalate 30% 34.31 22.03 - - - -
T28 Valifenalate 60% 37.25 23.73
T29 Difenoconazole 5% 10.78 14.41
T30 Difenoconazole 15% 12.75 16.10 - - - -
T31 Difenoconazole 50% 18.63 16.95 - - - -
T32 Untreated control - - - - - - -
It is clearly evident from the data shown in above Table 1 for per cent early blight disease control and per cent late blight disease control in tomato crop that the ternary composition of the present invention comprising Trifloxystrobin, Valifenalate and Difenoconazole Tl to T5 are highly synergistic for both the diseases control with > 1 Colby's Ratio. The binary combination of two fungicides Trifloxystrobin + Valifenalate (T6 to T10), Trifloxystrobin + Difenoconazole (Tl 1 to T15), and Valifenalate + Difenoconazole (T16 to T20) are compatible

but synergistic effect shown is low for the control of both the diseases as compared to ternary combination of Trifloxystrobin, Valifenalate and Difenoconazole.
Bio-efficacy of composition of present invention:
Example 14: Evaluation of composition of the present invention on tomato
The synergistic composition of the present invention Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC @ 400, 500 and 625 ml/ha along with different binary combinations and solo formulation were evaluated on tomato crop variety Pusa Rubi. The experiment was conducted at a farmer field, Sonipat during July to October, 2021 following Randomized Block Design (RBD) with three replications and maintaining a distance of 30 cm x 30 cm between plants and rows. The application of treatments was started with the initiation of early blight and late blight diseases incidence on the crop in experimental plots and repeat application was done after 15 days, under field conditions, the basic test composition as Trifloxystrobin 10% SC, Valifenalate 20% SC and Difenoconazole 12.5% SC were manufactured specifically by the inventor. For trinary combination, Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC was used and for binary combination, Trifloxystrobin 10% + Valifenalate 20% SC, Trifloxystrobin 10% + Difenoconazole 12.5% SC and Valifenalate 20% + Difenoconazole 12.5% SC were used. The solo formulations of Trifloxystrobin 10% SC, Valifenalate 20% SC were used for comparison. The solo formulation of Difenoconazole 25% EC and other binary combination formulations Tebuconazole 50% + Trifloxystrobin 25% WG and Azoxystrobin 18.2% + Difenoconazole 11.4% SC available in the market were also used for comparison.
Details of the Experiment:
1. Bio-efficacy evaluation against early blight disease on tomato crop
2. Bio-efficacy evaluation against late blight disease on tomato crop
3. Bio-efficacy evaluation based on per cent reduction in early blight disease incidence
4. Bio-efficacy evaluation based on per cent reduction in late blight disease incidence
5. Bio-efficacy evaluation based on tomato fruit yield
6. Phytotoxicity evaluation on tomato crop
7. Economics of treatments based on Cost: Benefit Ratio
Treatment details:

Tl - Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC @ 400 ml/ha
T2 - Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC @ 500 ml/ha
T3 - Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC @ 625 ml/ha
T4 - Trifloxystrobin 10% + Valifenalate 20% SC @ 400 ml/ha
T5 - Trifloxystrobin 10% + Valifenalate 20% SC @ 500 ml/ha
T6 - Trifloxystrobin 10% + Valifenalate 20% SC @ 625 ml/ha
T7 - Trifloxystrobin 10% + Difenoconazole 12.5% SC @ 400 ml/ha
T8 - Trifloxystrobin 10% + Difenoconazole 12.5% SC @ 500 ml/ha
T9 - Trifloxystrobin 10% + Difenoconazole 12.5% SC @ 625 ml/ha
T10 - Valifenalate 20% + Difenoconazole 12.5% SC @ 400 ml/ha
Til - Valifenalate 20% + Difenoconazole 12.5% SC @ 500 ml/ha
T12 - Valifenalate 20% + Difenoconazole 12.5% SC @ 625 ml/ha
T13 - Trifloxystrobin 10% SC @ 500 ml/ha
T14 - Valifenalate 20% SC @ 500 ml/ha
T15 - Difenoconazole 25% EC @ 250 ml/ha
T16 - Tebuconazole 50% + Trifloxystrobin 25% WG @ 350 ml/ha
T17 - Azoxystrobin 18.2% + Difenoconazole 11.4% SC @ 500 ml/ha
T18 - Untreated control
T19 - Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC @ 1000 ml/ha (For
phytotoxicity evaluation)
Methodology:
The observations for early blight disease caused by Alternaria solani and late blight disease caused by Phytophthora infestans were recorded before first spray and at 10 days after each spray based on three leaves of randomly selected ten plants per plot following 0-9 scale as under.
0-9 Scale for early blight disease
Score Symptoms
0 No symptom on the leaf
1 Small irregular brown spots covering 1% or less of the leaf area
, Small, irregular, brown spots with concentric rings covering 1 - 10% of the leaf
area ^ Lesions enlarging, irregular, brown with concentric rings, cover 11 - 25% of leaf
area

7 Lesions coalesce to form irregular, dark brown patches with concentric rings covering 26 - 50% of leaf area. Lesions on stems and petioles
q Lesions coalesce to form irregular, dark brown patches with concentric rings covering more than 51% of leaf area. Lesions on stems and petioles
0-9 Scale for late blight disease
Score Symptoms
0 No symptoms observed
1
Only a few plants affected here and there, upto 1 or 2 spots in 12 yards' radius. Upto 10 spots per plant, or general light spotting
3 About 50 spots per plant, or upto 1 leaflet in 10 attacked
5
Nearly every leaflet with lesions, plants still retaining normal form; field may smell of blight but looks green, although every plant is affected
Every plant affected and about V2 of leaf area destroyed by blight; field looks green flecked with brown
About 3/4 of leaf area destroyed by blight; field looks either predominantly brown or green. In some varieties the youngest leaves escape infection. Only few leaves left green but stem remain green. All leaves dead, stems dead or drying.
Based on the data recorded per cent disease index (PDI) was calculated. The per cent reduction in disease incidence was also calculated over control. The crop yield was recorded at each harvest and cumulative yield has been expressed as q/ha. The observations for phytotoxicity symptoms on tomato crop at 1, 3, 7 and 10 days after each spray were also recorded. Based on treatment application cost, market price of produce and net profit, the Cost: Benefit Ratio was calculated for the economics of ternary combination treatments. The data were subjected to statistically analysis of variance. Results are presented in Tables 2, 3 and 4.
Results:
The results for early blight and late blight disease incidence on tomato crop recorded during the experimental period and per cent reduction in disease incidence over untreated control are summarized in Table 2 and 3. The data on yield is presented in Table 4. The observations recorded for phytotoxicity symptom is presented in Table 5.
Table 2: Field bio-efficacy evaluation of Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC against early blight disease of tomato crop

s.
No. A.I. Formulation Per cent

Treatment dose dose (ml or PDI early blight disease

(g/ha) g/ha) reduction

Before spray 10
days
after I
spray 10 days after
II spray 10 days after
I spray 10 days after
II spray
Tl Trifloxystrobin 10% + Valifenalate 20% +
Difenoconazole 12.5% SC 40 +
80 +
50 400 6.54 (14.79) 7.41 (15.77) 7.65 (16.05) 55.56 73.95
T2 Trifloxystrobin 10% + Valifenalate 20% +
Difenoconazole 12.5% SC 50 + 100 + 62.5 500 6.67 (14.90) 7.04 (15.36) 6.67 (14.95) 57.78 77.31
T3 Trifloxystrobin 10% + Valifenalate 20% +
Difenoconazole 12.5% SC 62.5
+ 125+ 78.13 625 6.79 (15.07) 6.91 (15.24) 6.54 (14.80) 58.52 77.73
T4 Trifloxystrobin 10% + Valifenalate 20% SC 40 + 80 400 6.91 (15.17) 8.27 (16.68) 9.38 (17.81) 50.37 68.07
T5 Trifloxystrobin 10% + Valifenalate 20% SC 50 + 100 500 7.41 (15.78) 8.02 (16.44) 8.89 (17.34) 51.85 69.75
T6 Trifloxystrobin 10% + Valifenalate 20% SC 62.5 + 125 625 7.04 (15.31) 7.90 (16.30) 8.40 (16.84) 52.59 71.43
T7 Trifloxystrobin 10% +
Difenoconazole 12.5% SC 40 + 50 400 7.28 (15.64) 8.52 (16.97) 9.01 (17.46) 48.89 69.33
T8 Trifloxystrobin 10% +
Difenoconazole 12.5% SC 50 + 62.5 500 6.30 (14.50) 8.27 (16.69) 8.77 (17.21) 50.37 70.17
T9 Trifloxystrobin 10% +
Difenoconazole 12.5% SC 62.5
+ 78.13 625 7.16 (15.49) 8.15 (16.57) 8.52 (16.95) 51.11 71.01
T10 Valifenalate
20% + 80 + 50 400 6.67 (14.89) 8.64 (17.07) 9.75 (18.20) 48.15 66.81

Difenoconazole 12.5% SC
Til Valifenalate 20% +
Difenoconazole 12.5% SC 100 + 62.5 500 6.42 (14.64) 8.40 (16.83) 9.51 (17.93) 49.63 67.65
T12 Valifenalate 20% +
Difenoconazole 12.5% SC 125+ 78.13 625 6.91 (15.19) 8.27 (16.69) 9.38 (17.83) 50.37 68.07
T13 Trifloxystrobin 10% SC 50 500 7.04 (15.37) 10.00 (18.40) 11.98 (20.24) 40.00 59.24
T14 Valifenalate 20% SC 100 500 7.16 (15.51) 9.51 (17.95) 10.62 (19.01) 42.96 63.87
T15 Difenoconazole 25% EC 62.5 250 6.79 (15.04) 10.25 (18.64) 11.60 (19.90) 38.52 60.50
T16 Tebuconazole 50% +
Trifloxystrobin 25% WG 175 + 87.5 350 7.04 (15.34) 7.90 (16.31) 9.14 (17.59) 52.59 68.91
T17 Azoxystrobin 18.2% + Difenoconazole 11.4% SC 92.22
+ 57.76 500 6.67 (14.93) 8.02 (16.44) 9.26 (17.71) 51.85 68.49
T18 Untreated control - - 6.91 (15.23) 16.67 (24.08) 29.38
(32.82) - -
SEm± 0.83 0.60 0.50
CD (P=0.05) NS 1.71 1.44
Figures in parentheses are angular transformed values, NS -Non significant
Table 3: Field bio-efficacy evaluation of Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC against late blight disease of tomato crop

Treatment A.I.
dose (g/ha) Formulation
dose (ml or
g/ha) PDI early blight Per cent
disease
reduction
s.
No.


Before spray 10
days
after I
spray 10 days after
II spray 10 days after
I spray 10 days after
II spray
Tl Trifloxystrobin 10% + Valifenalate 20% +
Difenoconazole 12.5% SC 40 +
80 +
50 400 4.69 (12.48) 6.54 (14.81) 6.79 (15.10) 58.59 75.88
T2 Trifloxystrobin 10% + Valifenalate 50 + 100 + 62.5 500 4.81 (12.66) 6.17 (14.38) 5.43 (13.45) 60.94 80.70

20% +
Difenoconazole 12.5% SC
T3 Trifloxystrobin 10% + Valifenalate 20% +
Difenoconazole 12.5% SC 62.5 + 125+ 78.13 625 5.06 (12.98) 6.05 (14.23) 5.31 (13.31) 61.72 81.14
T4 Trifloxystrobin 10% + Valifenalate 20% SC 40 + 80 400 5.19 (13.15) 7.41 (15.77) 8.15 (16.57) 53.13 71.05
T5 Trifloxystrobin 10% + Valifenalate 20% SC 50 + 100 500 5.93 (14.08) 7.04 (15.38) 7.65 (16.05) 55.47 72.81
T6 Trifloxystrobin 10% + Valifenalate 20% SC 62.5 + 125 625 5.31 (13.29) 6.91 (15.23) 7.16 (15.52) 56.25 74.56
T7 Trifloxystrobin 10% +
Difenoconazole 12.5% SC 40 + 50 400 5.06 (12.99) 7.65 (16.05) 7.78 (16.18) 51.56 72.37
T8 Trifloxystrobin 10% +
Difenoconazole 12.5% SC 50 + 62.5 500 4.81 (12.65) 7.41 (15.78) 7.53 (15.92) 53.13 73.25
T9 Trifloxystrobin 10% +
Difenoconazole 12.5% SC 62.5 + 78.13 625 5.68 (13.76) 7.28 (15.65) 7.28 (15.65) 53.91 74.12
no Valifenalate 20% +
Difenoconazole 12.5% SC 80 + 50 400 4.81 (12.67) 8.15 (16.57) 8.52 (16.97) 48.44 69.74
Til Valifenalate 20% +
Difenoconazole 12.5% SC 100 + 62.5 500 4.57 (12.33) 8.02 (16.45) 8.27 (16.71) 49.22 70.61
T12 Valifenalate 20% +
Difenoconazole 12.5% SC 125+ 78.13 625 5.31 (13.30) 7.78 (16.17) 8.15 (16.58) 50.78 71.05
T13 Trifloxystrobin 10% SC 50 500 5.06 (12.99) 9.38 (17.80) 10.74 (19.12) 40.63 61.84
T14 Valifenalate 20% SC 100 500 5.56 (13.62) 8.64 (17.09) 9.38 (17.83) 45.31 66.67

ri5 Difenoconazole 25% EC 62.5 250 5.19 (13.12) 9.14 (17.58) 10.37 (18.77) 42.19 63.16
ri6 Tebuconazole 50% +
Tnfloxystrobin 25% WG 175 + 87.5 350 4.81 (12.67) 7.28 (15.64) 7.90 (16.32) 53.91 71.93
ri7 Azoxystrobin 18.2% + Difenoconazole 11.4% SC 92.22
+ 57.76 500 5.31 (13.30) 7.53 (15.91) 8.02 (16.45) 52.34 71.49
ri8 Untreated control - - 4.94 (12.83) 15.80 (23.41) 28.15 (32.04) - -
SEm± 0.52 0.49 0.40
CD (P=0.05) NS 1.40 1.16
Figures in parentheses are angular transformed values, NS -Non significant
Table 4: Field bio-efficacy evaluation of Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC based on tomato fruit yield and Cost: Benefit Ratio

s.
No. Treatment A.I.
dose (g/ha) Formulation
dose (ml or
g/ha) Yield (q/ha) Per cent increase in yield over
control C:B
Ratio
Tl Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC 40 +
80 +
50 400 174.33 (13.20) 44.48 1 :
20.76
T2 Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC 50 + 100 + 62.5 500 183.17 (13.53) 51.80 1 :
22.44
T3 Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC 62.5 + 125+ 78.13 625 185.67 (13.62) 53.87 1 : 21.29
T4 Trifloxystrobin 10% + Valifenalate 20% SC 40 + 80 400 167.50 (12.94) 38.81 1 : 17.99
T5 Trifloxystrobin 10% + Valifenalate 20% SC 50 + 100 500 168.83 (12.99) 39.92 1 : 17.06
T6 Trifloxystrobin 10% + Valifenalate 20% SC 62.5 + 125 625 170.50 (13.06) 41.30 1 : 16.09
T7 Trifloxystrobin 10% + Difenoconazole 12.5% SC 40 + 50 400 155.83 (12.48) 29.14 1 : 13.26
T8 Trifloxystrobin 10% + Difenoconazole 12.5% SC 50 + 62.5 500 157.00 (12.53) 30.11 1 : 12.63

T9 Trifloxystrobin 10% + Difenoconazole 12.5% SC 62.5 + 78.13 625 160.50 (12.67) 33.01 1 : 12.66
T10 Valifenalate 20% + Difenoconazole 12.5% SC 80 + 50 400 152.33 (12.34) 26.24 1 : 11.84
Til Valifenalate 20% + Difenoconazole 12.5% SC 100 + 62.5 500 154.50 (12.43) 28.04 1 : 11.69
T12 Valifenalate 20% + Difenoconazole 12.5% SC 125+ 78.13 625 157.50 (12.55) 30.52 1 : 11.63
T13 Trifloxystrobin 10% SC 50 500 144.67 (12.03) 19.89 1 : 8.60
T14 Valifenalate 20% SC 100 500 147.33 (12.12) 22.10 1 : 8.41
T15 Difenoconazole 25% EC 62.5 250 141.50 (11.89) 17.27 1 : 10.36
T16 Tebuconazole 50% + Trifloxystrobin 25% WG 175 + 87.5 350 152.83 (12.36) 26.66 1 : 14.95
T17 Azoxystrobin 18.2% + Difenoconazole 11.4% SC 92.22
+ 57.76 500 155.50 (12.47) 28.87 1 : 6.46
T18 Untreated control - - 120.67 (10.98) - -
SEm± 0.20
CD (P=0.05) 0.58
Figures in parentheses are square root transformed values
Table 5: Phyto toxicity evaluation of Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC on tomato crop

s.
No. Treatment A.I.
dose (g/ ha) Formu-lation dose (ml or g/ha) PI
(me anc lytotoxicity parameters observed*
:an data recorded at 1, 3, 7 10 days after each spray)



L W N V E H
Tl Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC 40 + 80 + 50 400 0 0 0 0 0 0
T2 Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC 50 + 100 + 62.5 500 0 0 0 0 0 0
T3 Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC 62.5 + 125+ 78.13 625 0 0 0 0 0 0
T4 Trifloxystrobin 10% + Valifenalate 20% SC 40 + 80 400 0 0 0 0 0 0

T5 Trifloxystrobin 10% + Valifenalate 20% SC 50 + 100 500 0 0 0 0 0 0
T6 Trifloxystrobin 10% + Valifenalate 20% SC 62.5 + 125 625 0 0 0 0 0 0
T7 Trifloxystrobin 10% + Difenoconazole 12.5% SC 40 + 50 400 0 0 0 0 0 0
T8 Trifloxystrobin 10% + Difenoconazole 12.5% SC 50 + 62.5 500 0 0 0 0 0 0
T9 Trifloxystrobin 10% + Difenoconazole 12.5% SC 62.5 + 78.13 625 0 0 0 0 0 0
T10 Valifenalate 20% + Difenoconazole 12.5% SC 80 + 50 400 0 0 0 0 0 0
Til Valifenalate 20% + Difenoconazole 12.5% SC 100 + 62.5 500 0 0 0 0 0 0
T12 Valifenalate 20% + Difenoconazole 12.5% SC 125+ 78.13 625 0 0 0 0 0 0
T13 Trifloxystrobin 10% SC 50 500 0 0 0 0 0 0
T14 Valifenalate 20% SC 100 500 0 0 0 0 0 0
T15 Difenoconazole 25% EC 62.5 250 0 0 0 0 0 0
T16 Tebuconazole 50% + Trifloxystrobin 25% WG 175 + 87.5 350 0 0 0 0 0 0
T17 Azoxystrobin 18.2% + Difenoconazole 11.4% SC 92.22 + 57.76 500 0 0 0 0 0 0
ri8 Untreated control - 0 0 0 0 0 0
ri9 Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC 100 +
200 +
125 1000 0 0 0 0 0 0
^ - Leaf injury on tips/ surface, W - Wilting, N - Necrosis, V - Vein clearing, E - Epinasty, H - Hyponasty
*Based on 0-10 scale where: 0=0%, 1=1-10%, 2=11-20%, 3=21-30%, 4=31-40%, 5=41-50%, 5=51-60%, 7=61-70%, 8=71-80%, 9=81-90%, 10=91-100%
From the above examples, it can be clearly seen that the composition of the present invention is superior for controlling the early blight and late blight diseases at 10 days after each spray (Table 2 and 3) in tomato crop as compared to the binary composition and solo component comprising Trifloxystrobin 10% SC, Valifenalate 20% SC and Difenoconazole 25% EC at different dosage levels. Other registered products available in the market, Tebuconazole 50% + Trifloxystrobin 25% WG, and Azoxystrobin 18.2% + Difenoconazole 11.4% SC, evaluated were next in order of effectiveness after ternary combination products.
The yield of tomato fruit also improved in the ternary combination of Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC as compared to binary and solo components (Table 4). One of the reasons of higher crop yield may be due to better control of diseases. The

Cost: Benefit Ratio revealed that ternary combination Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC @ 500 ml/ha and 625 ml/ha were most economical with higher Cost: Benefit Ratio as compared to other binary and solo treatments (Table 4).
The composition Trifloxystrobin 10% + Valifenalate 20% + Difenoconazole 12.5% SC @ 400, 500, 625 and 1000 ml/ha, further showed no phytotoxicity in the tomato crop after 1, 3, 7 and 10 days after each spray (Table 5).
It is evident from the above tables that the composition of the present invention resulted in efficient control of early blight and late blight diseases with higher yield as compared to the reference standard products (binary compositions and solo products). The Cost: Benefit Ratio also showed that ternary composition of the present invention was most economical with higher Cost: Benefit Ratio as compared to other binary and solo treatments. Further, the composition of present invention is resulted synergistic.
From the above investigations, it will be observed that numerous modifications and variations can be effectuated without departing from the true spirit and scope of the novel concepts of the present invention. It is to be understood that no limitations with respect to the specific embodiments illustrated is intended or should be inferred. It should be understood that all such modifications and improvements have been deleted herein for the sake of conciseness and readability but are properly within the scope of the following claims.
Advatages of the present invention:
1. A significantly lower incidence of early blight and late blight disease in tomato crops.
2. Improved yield, economical i.e., higher Cost: Benefit ratio.
3. No phytotoxic effect.
4. Highly effective in improving yield, and controlling dieases at lower doses.

We claim:

1. A synergistic fungicidal composition comprising, as active ingredients, (a) Valifenalate or its agro chemically acceptable salts; (b) at least one fungicide selected from Strobilurin fungicides or their agro chemically acceptable salts; and (c) at least one fungicide selected from azole fungicides or their agro chemically acceptable salts.
2. The fungicidal composition as claimed in claim 1, wherein the at least one Strobilurin fungicide is selected from Azoxystrobin, Pyraclostrobin, Fluoxastrobin, Kresoxim-methyl, Trifloxystrobin, Picoxystrobin, Mandestrobin, and Metominostrobin or their agro chemically acceptable salts.
3. The synergistic fungicidal composition as claimed in claim 1, wherein the azole fungicides is selected form Imazalil, Oxpoconazole Fumarate, Pefurazoate, Prochloraz, Triflumizole, Azaconazole, Bromuconazole, Bitertanol, Cyproconazole, Diclobutrazol, Difenoconazole, Diniconazole, Epoxiconazole, Etaconazole, Fenbuconazole, Fluquinconazole, Flusilazole, Flutriafol, Furconazole, Hexaconazole, Imibenconazole, Ipconazole, Metconazole, Myclobutanil, Penconazole, Propiconazole, Prothioconazole, Quinconazole, Simeconazole, Tebuconazole, Tetraconazole, Triadimefon, Triadimenol or Triticonazole or their agro chemically acceptable salts.
4. The fungicidal composition as claimed in claim 1, wherein the composition comprises of:
a. valifenalate or its agrochemically acceptable salts in an amount in the range
from 1 to 60% by weight of the composition;
b. at least one strobilurin fungicide or its agrochemically acceptable salts in an
amount in the range from 1 to 40% by weight of the composition; and
c. at least one azole fungicide or its agrochemically acceptable salts in an amount
in the range from 5 to 50% by weight of the composition.
5. The fungicidal composition as claimed in claim 1, wherein the composition comprises
of:
a. Valifenalate or its agrochemically acceptable salts in an amount in the range from 1 to 60%) by weight of the composition;

b. Trifloxystrobin or its agrochemically acceptable salts as strobilurin fungicide in
an amount in the range from 1 to 40% by weight of the composition; and
c. at least one azole fungicide or its agrochemically acceptable salts in an amount
in the range from 5 to 50% by weight of the composition.
The fungicidal composition as claimed in claim 1, wherein the composition comprises of:
a. Valifenalate or its agrochemically acceptable salts in an amount in the range
from 1 to 60%) by weight of the composition;
b. Trifloxystrobin or its agrochemically acceptable salts as strobilurin fungicide in
an amount in the range from 1 to 40% by weight of the composition; and
c. Difenoconazole or its agrochemically acceptable salts in an amount in the range
from 5 to 50% by weight of the composition.
The fungicidal composition as claimed in any of the claims 1 to 6, wherein the composition comprises of:
a. Valifenalate or its agrochemically acceptable salts in an amount in the range
from 10 to 30%) by weight of the composition;
b. Trifloxystrobin or its agrochemically acceptable salts as strobilurin fungicide in
an amount in the range from 5 to 20% by weight of the composition; and
c. Difenoconazole or its agrochemically acceptable salts in an amount in the range
from 5 to 25% by weight of the composition.
The fungicidal composition as claimed in any one of the claims 1 to 7, which further comprises one or more agro chemically acceptable excipients.
The fungicidal composition as claimed in any one of the claims 1 to 7, wherein the agrochemically acceptable excipients are selected from group comprising surfactants/dispersing agents, polymers, anti-freezing agent, wetting agents, anti-foaming agents, colorants, pigments, binder/structuring agents, biocides/anti-microbial agent, thickeners, emulsifiers, buffering agent, fillers, diluents/solvents, quick coating agents or sticking agents, preservatives, adjuvants and solvents or a combination thereof.

10. The fungicidal composition as claimed in claim 9, wherein the thickeners are selected from the group comprising silicates such metal silicates, aluminum magnesium silicate sodium carboxy methyl cellulose, methyl cellulose, ethyl cellulose, poly vinyl alcohol, sodium alginate, sodium poly acrylate, xanthan gum, welan gum, gum arabic, montmorillonite, lingo sulfonates, hydroxy methyl cellulose, dextrin, starch, Bentonite Clay, Polysaccharides or combinations thereof.
11. The fungicidal composition as claimed in claim 9, wherein the anti-freezing agent is selected from the group comprising polyethylene glycols, methoxy polyethylene glycols, propylene glycol, polypropylene glycols, polybutylene glycols, Mono ethylene glycol, glycerin, ethylene glycol, Glycerin, Diethylene glycol or combinations thereof.
12. The fungicidal composition as claimed in claim 9, wherein the anti-foaming agent is selected from the group comprising silicone oils, or mineral oils, Fatty acid ester; potassium sorbate, and parahydroxy benzoates, silicon emulsion based anti-foam agents, Siloxane polyalkylene oxide, Polydimethyl Siloxane, tri siloxane ethoxylates or combinations thereof.
13. The fungicidal composition as claimed in claim 9, wherein the wetting agent is selected from the group comprising Tristyrylphenol ethoxylate nonionic emulsifier, Mixture of non-ionic surfactants & Alkoxylated Alcohol/Block copolymer, sodium lauryl sulphate; sodium dioctyl sulphosuccinate; Tristyrylphenol ethoxylate non-ionic emulsifier/ mixture of non-ionic surfactants and alkoxylated Alcohol/Block copolymer, ionic surfactant mixtures, anionic surfactants, alkyl phenol ethoxylates, aliphatic alcohol ethoxylates and the salts or combinations thereof.
14. The fungicidal composition as claimed in claim 9, wherein the polymers are selected from the group comprising polyvinyl alcohols, polyvinylpyrrolidone, gel-forming carraagheenans, water-soluble gelatine and casein, superabsorbent polymers such polyacrylamides and polyacrylates based polymers, semi-synthetic or fully-synthetic peptide/protein-based superabsorbent polymers such as collagen-based synthetic polymers, elastin-like polypeptides, polyaspartic acid, polyaspartates, polyglutamic acid, polyglutamate, Semi-synthetic or fully-synthetic polysaccharide: carboxymethyl

starch , sulfoethyl starch, carboxymethyl cellulose, sulfoethyl cellulos, hydroxypropyl cellulose, hydroxyethyl cellulose, methylcellulose, chitosan, acrylonitrile, acrylic acid, methacrylic acid, acrylamide, methacrylamide, 2-acrylamido-2-methyl-propanesulfonic acid (AMPS), vinyl sulfonic acid, ethyl acrylate, and potassium acrylate or combinations thereof.
15. The fungicidal composition as claimed in claim 9, wherein the adjuvant is selected from the group comprising Silicone Ethoxylated Oil, Polyvinyl Pyrrolidon, Poly vinyl Alcohol, Blend of poly terpene resin or combination thereof.
16. The fungicidal composition as claimed in claim 9, wherein the diluent/solvents are selected from the group comprising lactose, glucose, fructose, maltose, sucrose, in the anhydrous or hydrate forms, urea, water soluble or dispersible polymers, water soluble inorganic salts, Demineralized (DM) water, Vegetable Oil or combination thereof.
17. The fungicidal composition as claimed in claim 9, wherein the filler is selected from the group comprising Silicon Dioxide, China -Clay, Kaolin, Talc, starch or combination thereof.
18. The fungicidal composition as claimed in claim 9, wherein the biocide/anti-microbial agent is selected from the group comprising Benzisothiazolin-3-one / Formaldehyde/Sodium benzoate/Sodium o-phenyl phenate, 5-chloro-2-methyl-4-isothiazolin-3-one & 2-methyl-4-isothiazolin-3-one or combination thereof.
19. The fungicidal composition as claimed in any one of the claims 1 to 18, wherein the composition is in form selected from Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable Slurry (FS), Flowable Suspension(FS), Suspension Concentrate (SC), Suspension concentrate for direct application (SD), Suspo- emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Water soluble powder (SP), Water dispersible powder for slurry seed treatment (WS), Water dispersible granules (WDG), Wettable powders (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible

tablet (WT), granular (GR), oil in dispersion (OD), a mixed formulation of CS and SC (ZC) or a mixed formulation of CS and SE (ZE), a mixed formulation of CS and EW (ZW).
20. The fungicidal composition as claimed in any one of the claims 1 to 18, wherein the preferred composition is in form of Suspension Concentrate (SC), Water dispersible granules (WDG/WG), Suspo- emulsion (SE), or Suspo- emulsion (SE).
21. A method for controlling a plant disease caused by a fungal plant pathogens comprising applying to the plant or portion thereof or soil, a fungicidally effective amount of the fungicidal composition as claimed in any one of the claims 1 - 20.
22. A method of improving vigour or yield of the plant comprising, treating a plant or portion thereof or soil, a fungicidally effective amount of the fungicidal composition as claimed in any one of the claims 1 - 20.
23. The method as claimed in claim 21 or 22, wherein the plant is selected from the group consisting of cereals, field crops, seeds, fruits, tree nuts, vegetables, turf grasses, potatoes, tomatoes, grapes and ornamentals.
24. A process for preparing a stable synergistic fungicidal composition as claimed in claims 1-20.