The present invention relates to synergistic fungicidal composition. More particularly, the present invention relates to synergistic fungicidal composition comprising an acylamino acid fungicide or its agrochemically acceptable salts and at least one fungicide selected from Strobilurin fungicides or their agrochemically acceptable salts. The present invention also relates to a process for preparation of such synergistic fungicidal composition.
BACKGROUND OF THE INVENTION
Fungicides, are compounds of natural or synthetic origin, which act to protect plants against damage caused by fungi. Fungicides are used extensively in modern agricultural practices. In fact, some crops cannot be grown productively without the use of fungicides. Using fungicides allows a grower to increase crop yield and quality, and thus increase the crop value. In most cases, the increase in crop value is worth at least three times the cost of the fungicide.
Most crop and ornamental plants are subject to attack by several fungi. Plant diseases due to fungi cause significant productivity losses in ornamentals, vegetable, field, cereal and fruit crops, resulting in higher consumer cost. Plant diseases can be difficult to control and, in addition to being highly destructive, may develop resistance to commercial fungicides. Due to the diversity of these organisms and their potential for inciting serious disorders in combination or alone, has resulted in the need for broad-spectrum disease control. Although there are numerous chemical compounds (fungicides) available to aid in prevention of diseases of plants, each of these has practical deficiencies, which restrict its use. Fungicide combinations are frequently used to improve disease control, broaden the spectrum of control, and slow resistance development. Thus, new advantageous combinations are required to provide a variety of options to best meet specific plant disease control needs. Furthermore, certain uncommon fungicide combination exhibits a greater-than-additive (i.e. synergistic) effect to providing commercially significant levels of plant disease control.
When two or more substances in combination demonstrate unexpectedly high biological activity, for example fungicidal activity, the resulting phenomenon is known as synergism.
Conventional fungicide compositions comprising acylamino acid fungicide with anti-peronosporic activity usually fail to provide a practically sufficient control effect on plant diseases depending on the application situation, either because it has an insufficient effect on

specific plant diseases or its residual effectiveness lasts for a relatively short period of time. As a result, the number of fungal diseases and fungi that acylamino acid fungicides can control and treat is limited. Furthermore, when an acylamino acid fungicide is used for an extended period of time, the risk of resistance development increases.
To achieve high crop productivity, it is important to protect the plant from damage caused by plant pathogenic (disease-causing) organisms such as various types of plant fungal infections. However, there exists a continuous need in the art for improved compositions that not only aids in control of fungal growth but also improve bio-efficacy, including increased plant yield, reduced phytotoxicity, and an improved stability characteristics as well as economic significance such as ease of manufacture with lower costbenefit ratio i.e. higher return on investment. Therefore, there is a long felt need for novel and effective synergistic fungicidal composition comprising acylamino acid fungicide for controlling the harmful pests, fungi in plants that demonstrate high efficacy, high selectivity, are environmentally safe and can be advantageously formulated using reduced dosages of individual fungicides/technicals in the final composition.
Fungicidal combinations have significant advantages over individual applications, including improved and extended fungal control, lower fungicide application rates and costs, shorter contact times for improved results, less stringent use restrictions, improved selectivity, a broader spectrum of fungi controlled, lower cost and reduced residue problems. However, determining appropriate fungicide application rates and combination ratio is critical for disease control efficacy. In view of the above, developing a fungicidal composition containing an acylamino acid fungicide that exhibits no cross-resistance to the existing fungicidal agents, no toxicity issues, is stable, offers broad spectrum control of fungal diseases, reduces pest resistance development and little negative environmental impact is extremely difficult.
Various prior arts have offered various fungicidal compositions useful for protecting the plant from damage by harmful pests such as Downy mildew, Crown rot, Late blight, and so on over the years. Some of such prior arts are indicated below:
US20180160684A1 discloses a fungicide composition containing, as active ingredients, (a) Valifenalate or a salt thereof and (b) at least one fungicide selected from the group consisting

of Isofetamid, Pyriofenone, and azole-based compounds; and a method for controlling a plant disease, which includes applying the fungicide composition to a plant or soil.
CN108353921A discloses a bactericidal composition including Valifenalate and Fenpyrazamine, the weight ratio of two kinds of active ingredient is 1- 40 : 40-1, the weight percentage of active ingredient is 1- 90% in fungicide, preferably 5- 80%, remaining is the auxiliary agent and carrier that allow in pesticide.
Valifenalate, IUPAC name: methyl (3RS)-3-(4-chlorophenyl)-N-[N-(isopropoxycarbonyl)-L-valyl]-P-alaninate, anti-peronosporic fungicide, belonging to the group of Carboxylic acid (CAA) Amides, with strong systemic activity. Valifenalate belongs to acylamino acid class of fungicides. Fungi belonging to Oomycetes like Plasmopara viticola (grapevine), Phytotphthora infestans (potato and tomato), Peronospora destructor (onion), Pseudoperonospora cubensis (cucurbits), Bremia lactucae (lettuce and similar) are the exclusive target of Valifenalate (Valis). Valifenalate acts by inhibiting the cell metabolism and particularly the synthesis of the fungal cell wall. Particularly, it is an anti-peronosporic fungicide which is used to control mildew in many crops including grapes, potatoes and tomatoes.
CI
.0.
CH3 O X
H3CX CH3
Valifenalate
Strobilurin fungicides are globally used to combat white mold, rot, early and late leaf spot, rusts and rice blast (Bartett et al., 2001; FAO Meeting, 2008). Mushrooms (Basidiomycetes) are natural sources of strobilurins, and the first natural strobilurin compound, Strobilurin-A, was originally isolated from the mushroom Strobilurus tenacellus by Anke et al. (1977). Examples of strobilurin fungicides, include Azoxystrobin, Pyraclostrobin, Fluoxastrobin, Kresoxim-methyl, Trifloxystrobin, Picoxystrobin, Mandestrobin, and Metominostrobin, which have been developed and marketed (Rodrigues et al., 2013; Khandelwal et al., 2014). Strobilurin fungicides are also referred as Qol (Quinine outside inhibition) fungicides because of their unique mechanism of action. They specifically bind to the quinol oxidation (Qo) site of cytochrome b to inhibit mitochondrial respiration. This binding blocks electron transfer

between cytochrome b and cytochrome cl and inhibits the synthesis of nicotinamide adenine dinucleotide (NADH) oxidation and the mitochondrial membrane protein adenosine triphosphate (ATP). In particular, strobilurin fungicides work by inhibiting the fungi's ability undergo normal respiration.
Various strobilurin fungicides are known in the art. Chemical structure of some of such fungicides are provided below:



°^

C|—\ J—N:

*w

CX „ N.
o

cr

A zoxy strobin

Py r ac 1 o strob i n

F1 uoxa strobin

K resoxim -m elhyl

OFXr%
CF.

T riflo x y s hX3 b in

P icoxy strob in

Mandestrobin

Metomino strobin

Trifloxystrobin, in particular, is an Oximino-acetate fungicide with the target site code C-3 and is classified as a Qol- (quinone Outside inhibition) fungicide. Strobilurin fungicides have a broad spectrum of fungicidal activity, are cost effective, and degrade rapidly during plant metabolism. Such fungicides provide long-lasting, weather-protected disease control with high rainfastness.

Strobilurin fungicides are effective against a wide range of fungi and pests, including Leptosphaerulina and Curvularia species. Strobilurin fungicides are distinguished by their ability to strongly bond to plant surfaces via waxy layer penetration. This creates a safe fungicide reservoir for long-term, weather-protected disease control. These fungicides inhibit fungal spore germination and mycelial growth by interfering with respiration in plant pathogenic fungi. Fungicides can be formulated in a variety of forms, most notably liquid and solid compositions.
There have been no studies to determine the most-effective and synergistic ratios of fungicides derived from acylamino acid and at least one compound derived from strobilurin based compounds, at which ratio these fungicides complement each other's fungicidal properties not seen at other ratios. Thus there exists a need in the art for a composition comprising fungicides selected from acylamino acid class and at least one compound selected from strobilurin based compounds at a ratio that provides superior and effective control of fungal infections for control of various fungal diseases including early blight (caused by Alternaria solani) and late blight (caused by Phytophthora infestans) diseases.
Thus, it is preferable to develop compositions and processes for combinations of the fungicides listed above that can be used to treat a variety of pests and plant fungal diseases such as scab, powdery mildew, and downy mildews such as Phytotphthora species, Leptosphaerulina species, and Curvularia species. The present invention relates to a novel, synergistic composition and method for preparing the compositions containing the aforementioned active ingredients that are both stable and provide the desired bio-efficacy and synergistic effect.
OBJECTS OF THE INVENTION:
It is an object of the present invention to provide a novel and effective synergistic fungicidal composition demonstrating high efficacy and high selectivity.
It is another object of the present invention to provide a novel and effective synergistic fungicidal composition for controlling the harmful pests and fungi in plants and offers broad spectrum control of plant fungal diseases

It is another object of the present invention to provide a novel and effective synergistic fungicidal composition which is ideal for fungicide resistance management and enhanced disease control.
It is another object of the present invention to provide a novel and effective synergistic fungicidal composition which can be easily formulated with high stability.
It is another object of the invention to provide a novel process for preparing synergistic fungicidal composition comprising an acylamino acid fungicide and Strobilurin fungicide.
It is another object of the present invention to provide a novel and effective synergistic fungicidal composition which uses lesser amounts of the actives as compared to the actives when used alone.
It is another object of the present invention to provide novel and effective synergistic fungicidal composition with high degree of rain fastness having longer residual action on pathogens, thereby reduce the number of foliar spray applications.
It is another object of the present invention to provide a novel and effective synergistic fungicidal composition which is environmentally safe, possesses broad spectrum bio-efficacy, is synergistic, is less toxic in terms of phytotoxicity.
It is another object of the present invention to provide a novel and effective synergistic fungicidal composition which is stable at various temperature ranges and variable environmental changes.
Yet another object of the present invention is to provide a method of controlling fungi using fungicidal composition comprising an acylamino acid fungicide and at least one strobilurin fungicide.
The synergistic fungicidal composition of the present invention is found to be useful in protecting a wide range of crops like fruits, vegetables, cereals, flowers etc. against major diseases. The composition achieves improved biological efficacy by enhancing overall control of fungus over a shorter period of time. Additional benefits of using the fungicidal composition

of the present investigation include reduced risk of occupational hazard, lower cost of application, better cost: benefit ratio to the end user, reduced fuel and labour cost, saving in applicator's time and reduced wear of equipment and loss caused by mechanical damage to the crop and soil.
SUMMARY OF THE INVENTION
Accordingly, the present invention provides a fungicidal composition comprising synergistic and bio-effective amount of acylamino acid fungicide or its agrochemically acceptable salts and at least one compound selected from Strobilurin fungicides or their agrochemically acceptable salts.
Further, the present invention provides a synergistic fungicidal composition comprising, as active ingredients, (a) Valifenalate or its agrochemically acceptable salts and (b) at least one fungicide selected from Strobilurin fungicides or their agrochemically acceptable salts.
In an embodiment of the present invention, at least one Strobilurin fungicide is selected from Azoxystrobin, Pyraclostrobin, Fluoxastrobin, Kresoxim-methyl, Trifloxystrobin, Picoxystrobin, Mandestrobin, and Metominostrobin or its agrochemically acceptable salt.
In another embodiment of the present invention, the fungicidal composition comprises of:
a. valifenalate or its agro chemically acceptable salts in an amount in the range
from 1 to 80% by weight of the composition;
b. at least one strobilurin or its agro chemically acceptable salts in an amount in
the range from 1 to 40% by weight of the composition.
In another embodiment of the present invention, the composition comprises of:
a. Valifenalate or its agro chemically acceptable salts in an amount in the range
from 1 to 80%) by weight of the composition;
b. Trifloxystrobin or its agro chemically acceptable salts as strobilurin fungicide
in an amount in the range from 1 to 40% by weight of the composition.
In an embodiment of the present invention, the composition further comprises one or more agrochemically acceptable excipients.
In another embodiment of the present invention the agro chemically acceptable excipients are selected from group comprising surfactants/dispersing agents, polymers, anti-freezing agent,

wetting agents, anti-foaming agents, colorants, pigments, binder/structuring agents, biocides/anti-microbial agent, thickeners, emulsifiers, buffering agent, fillers, diluents/solvents, quick coating agents or sticking agents, preservatives, adjuvants and solvents or a combination thereof.
In another embodiment of the present invention, thickeners are selected from the group comprising silicates such metal silicates, aluminum magnesium silicate sodium carboxy methyl cellulose, methyl cellulose, ethyl cellulose, poly vinyl alcohol, sodium alginate, sodium poly acrylate, xanthan gum, welan gum, gum arabic, montmorillonite, lingo sulfonates, hydroxy methyl cellulose, dextrin, starch, Bentonite Clay, Polysaccharides or combinations thereof.
In another embodiment of the present invention, the anti-freezing agent is selected from the group comprising polyethylene glycols, methoxy polyethylene glycols, propylene glycol, polypropylene glycols, polybutylene glycols, Mono ethylene glycol, glycerin, ethylene glycol, Glycerin, Diethylene glycol or combinations thereof.
In another embodiment of the present invention, the anti-foaming agent is selected from the group comprising silicone oils, or mineral oils, Fatty acid ester; potassium sorbate, and parahydroxy benzoates, silicon emulsion based anti-foam agents, Siloxane polyalkylene oxide, Polydimethyl Siloxane, tri siloxane ethoxylates or combinations thereof.
In another embodiment of the present invention, the wetting agent is selected from the group comprising Tristyrylphenol ethoxylate nonionic emulsifier, Mixture of non-ionic surfactants & Alkoxylated Alcohol/Block copolymer, sodium lauryl sulphate; sodium dioctyl sulphosuccinate; Tristyrylphenol ethoxylate non-ionic emulsifier/ mixture of non-ionic surfactants and alkoxylated Alcohol/Block copolymer, ionic surfactant mixtures, anionic surfactants, alkyl phenol ethoxylates, aliphatic alcohol ethoxylates and the salts or combinations thereof.
In yet another embodiment of the present invention, the polymers are selected from the group comprising polyvinyl alcohols, polyvinylpyrrolidone, gel-forming carraagheenans, water-soluble gelatine and casein, superabsorbent polymers such polyacrylamides and polyacrylates based polymers, semi-synthetic or fully-synthetic peptide/protein-based superabsorbent

polymers such as collagen-based synthetic polymers, elastin-like polypeptides, polyaspartic acid, polyaspartates, polyglutamic acid, polyglutamate, Semi-synthetic or fully-synthetic polysaccharide: carboxymethyl starch , sulfoethyl starch, carboxymethyl cellulose, sulfoethyl cellulos, hydroxypropyl cellulose, hydroxyethyl cellulose, methylcellulose, chitosan, acrylonitrile, acrylic acid, methacrylic acid, acrylamide, methacrylamide, 2-acrylamido-2-methyl- propanesulfonic acid (AMPS), vinyl sulfonic acid, ethyl acrylate, and potassium acrylate or combinations thereof.
In another embodiment of the present invention, adjuvant is selected from the group comprising Silicone Ethoxylated Oil, Polyvinyl Pyrrolidon,Poly vinyl Alcohol,Blend of poly terpene resin or combination thereof.
In another embodiment of the present invention, diluent/solvents are selected from the group comprising lactose, glucose, fructose, maltose, sucrose, in the anhydrous or hydrate forms, urea, water soluble or dispersible polymers, water soluble inorganic salts, Demineralized (DM) water, Vegetable Oil or combination thereof.
In another embodiment of the present invention, filler is selected from the group comprising Silicon Dioxide, China -Clay, Kaolin, Talc, starch or combination thereof.
In another embodiment of the present invention, the biocide/anti-microbial agent is selected from the group comprising Benzisothiazolin-3-one / Formaldehyde/Sodium benzoate/Sodium o-phenylphenate, 5-chloro-2-methyl-4-isothiazolin-3-one & 2-methyl-4-isothiazolin-3-one or combination thereof.
In another embodiment of the present invention, the composition is in form composition is in form selected from Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable Slurry (FS), Flowable Suspension(FS), Suspension Concentrate (SC), Suspension concentrate for direct application (SD), Suspo-emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Water soluble powder (SP ), Water dispersible powder for slurry seed treatment (WS), Water dispersible granules (WDG), Wettable powders (WP), Water dispersible powder for slurry seed treatment (WS),

Water dispersible tablet (WT), granular (GR), oil in dispersion (OD), a mixed formulation of CS and SC (ZC) or a mixed formulation of CS and SE (ZE), a mixed formulation of CS and EW (ZW).
In another embodiment of the present invention, a method is provided for controlling a plant disease caused by a fungal plant pathogens comprising applying to the plant or portion thereof or soil, a fungicidally effective amount of the present invention fungicidal composition.
In another embodiment of the present invention provides a method of improving vigour or yield of the plant comprising, treating a plant or portion thereof or soil, a fungicidally effective amount of the fungicidal composition.
In another embodiment of the present invention, the plant is selected from the group consisting of cereals, field crops, seeds, fruits, tree nuts, vegetables, turf grasses, potatoes, tomatoes, grapes and ornamentals.
In yet another embodiment of the present invention, it provides a process for preparing a stable fungicidal composition comprising of as active ingredients, (a) Valifenalate or its agrochemically acceptable salts and (b) at least one fungicide selected from Strobilurin fungicides or their agrochemically acceptable salts.
In yet another embodiment of the present invention, adjuvants to be used in the formulation include solid carriers such as diatomaceous earth, slaked lime, calcium carbonate, talc, white carbon, kaolin, bentonite, a mixture of kaolinite and sericite, clay, sodium carbonate, sodium bicarbonate, mirabilite, zeolite, and starch; solvents such as water, toluene, xylene, solvent naphtha, dioxane, acetone, isophorone, methyl isobutyl ketone, chlorobenzene, cyclohexane, dimethyl sulfoxide, dimethylformamide, dimethylacetamide, N-methyl-2-pyrrolidone, and alcohols; anionic surfactants.
In yet another embodiment of the present invention, it provides a fungicidal composition comprising acylamino acid fungicide having anti-peronosporic activity and a strobilurin based compound selected from but not limited to Azoxystrobin, Pyraclostrobin, Fluoxastrobin, Kresoxim-methyl, Trifloxystrobin, Picoxystrobin, Mandestrobin, and Metominostrobin, which

specifically bind to the quinol oxidation (Qo) site of cytochrome b to inhibit mitochondrial respiration of fungal pests.
In another embodiment of the present invention, the fungicidal composition comprising an acylamino acid fungicide having anti-peronosporic activity and at least one compound selected from Strobilurin fungicide are present in synergistically effective weight ratio.
In another embodiment of the present invention, the invention provides a method for controlling pests including fungi in plants, said method comprising applying to the locus of the pest infestation a fungicidal composition comprising (a) Valifenalate or its agrochemically acceptable salts and (b) at least one fungicide selected from Strobilurin fungicides or their agrochemically acceptable salts.
In another embodiment of the present invention, the fungicidal composition of the present invention further comprises an agriculturally acceptable excipients selected from the group comprising surfactants/dispersing agents, polymers, anti-freezing agent, wetting agents, anti-foaming agents, colorants, pigments, binder/structuring agents, biocides/anti-microbial agent, thickeners, emulsifiers, buffering agent, fillers, diluents/solvents, quick coating agents or sticking agents, preservatives, adjuvants and solvents or a combination thereof. More generally, the active materials can be combined with any solid or liquid additive, which complies with usual formulation techniques.
DETAILED DESCRIPTION OF THE INVENTION
Embodiments, of the present disclosure, will now be described herein. Embodiments are provided so as to thoroughly and fully convey the scope of the present disclosure to the person skilled in the art. Numerous details are set forth, relating to specific components, and methods, to provide a complete understanding of embodiments of the present disclosure. It will be apparent to the person skilled in the art that the details provided in the embodiments should not be construed to limit the scope of the present disclosure. In some embodiments, well-known processes, well-known additional excipients, and well-known techniques are not described in detail. Discussed below are some representative embodiments of the present invention. The invention in its broader aspects is not limited to the specific details and representative methods.

All technical and scientific terms used herein have the same meanings as commonly understood by someone ordinarily skilled in the art to which the present subject matter belongs.
The terminology used, in the present disclosure, is only for the purpose of explaining a particular embodiment and such terminology shall not be considered to limit the scope of the present disclosure. As used in the present disclosure, the forms "a," "an," and "the" may be intended to include the plural forms as well, unless the context clearly suggests otherwise. Thus, for example, reference to a composition containing "a compound" includes a combination of two or more compounds. It should also be noted that the term "or" is generally employed in its sense including "and/or" unless the content clearly dictates otherwise. The terms "comprises," "comprising," "including," and "having," are open ended transitional phrases and therefore specify the presence of stated features, integers, steps, operations, elements, but do not forbid the presence or addition of one or more other features, integers, steps, operations, elements, components, and/or groups thereof. The particular order of steps disclosed in the method and process of the present disclosure is not to be construed as necessarily requiring their performance as described or illustrated. It is also to be understood that additional or alternative steps may be employed.
Variations or modifications to the composition of this invention, within the scope of the invention, may occur to those skilled in the art upon reviewing the disclosure herein. Such variations or modifications are well within the spirit of this invention.
The expression of various quantities in terms of "% w/w" or "%" means the percentage by weight, relative to the weight of the total solution or composition unless otherwise specified. The term "active ingredient" (a.i.) or "active agent" used herein refers to that component of the composition responsible for control and killing of pest.
The terms "plants" and "vegetation" include, but are not limited to, germinant seeds, emerging seedlings, plants emerging from vegetative propagules, and established vegetation.
The term "crop" shall include a multitude of desired crop plants or an individual crop plant growing at a locus.

The term "synergistic", as used herein, refers to the combined action of two or more active agents blended together and administered conjointly that is greater than the sum of their individual effects.
"Bioactive/ fungicidally effective amounts" as mentioned herein means that amount which, when applied for treatment of crops, is sufficient to give effect in such treatment.
The present invention provides a stable synergistic fungicidal composition comprising of acylamino acid and at least one strobilurin fungicide; and process of preparation thereof.
More particularly, the present invention provides a stable synergistic fungicidal composition comprising (a) Valifenalate or its agrochemically acceptable salts and (b) at least one fungicide selected from Strobilurin fungicides or their agrochemically acceptable salts.
In an embodiment of the present invention, the invention provides a fungicidal composition comprising synergistic and bio-effective amount of (a) Valifenalate or its agrochemically acceptable salts and (b) at least one fungicide selected from Strobilurin fungicides or their agrochemically acceptable salts.
In another embodiment of the present invention, the acylamino acid fungicide has anti-peronosporic activity.
In further embodiment of the present invention, acylamino acid fungicide is used in an amount of about 1-80% weight of the total composition
In another embodiment of the present invention, the acylamino acid fungicide is Valifenalate.
In further embodiment of the present invention, the strobilurin fungicide is used in an amount of about 1-40% weight of the total composition.
In another embodiment of the present invention, strobilurin fungicide is selected from Azoxystrobin, Pyraclostrobin, Fluoxastrobin, Kresoxim-methyl, Trifloxystrobin, Picoxystrobin, Mandestrobin, and Metominostrobin.
In an preferred embodiment of the present invention, the strobilurin fungicide is Trifloxystrobin.

In another embodiment of the present invention, provides a process for preparation of the said formulation wherein, the said formulation is selected from Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable Slurry (FS), Flowable Suspension(FS), Suspension Concentrate (SC), Suspension concentrate for direct application (SD), Suspo- emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Water soluble powder (SP ), Water dispersible powder for slurry seed treatment (WS), Water dispersible granules (WDG), Wettable powders (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), granular (GR), oil in dispersion (OD), a mixed formulation of CS and SC (ZC) or a mixed formulation of CS and SE (ZE), a mixed formulation of CS and EW (ZW).
In yet another embodiment of the present invention, the agriculturally acceptable excipients are selected from group comprising surfactants/dispersing agents, polymers, anti-freezing agent, wetting agents, anti-foaming agents, colorants, pigments, binder/structuring agents, biocides/anti-microbial agent, thickeners, emulsifiers, buffering agent, fillers, diluents/solvents, quick coating agents or sticking agents, preservatives, adjuvants and solvents or a combination thereof.
In another preferred embodiment of the present invention, the fungicidal composition comprising (a) Valifenalate or its agrochemically acceptable salts and (b) at least one fungicide selected from Strobilurin fungicides or their agrochemically acceptable salts, not only has enhances bio-efficacy and provides synergistic effect but also gives control of fungal pathogens for a longer duration as compared to solo formulations of acylamino acid fungicide such as Valifenalate and strobilurin based compound such as Trifloxystrobin.
In yet another embodiment of the present invention, there is high benefit: cost ratio.
In one embodiment, the composition according to the present invention acts synergistically to control various fungi in various crops.
In another preferred embodiment of the invention, yield, plant health and germination rate of the treated plant and crop is improved.

In another preferred embodiment of the invention, the yield and germination rate of the plants treated according to the method of the invention, increases synergistically.
In accordance with an embodiment of present invention, the composition is useful for effectively controlling the soil borne pests, improve the germination rate and also enhances the vigor/yield of the plant.
In another embodiment of the present invention the agro chemically acceptable excipients are selected from group comprising surfactants/dispersing agents, polymers, anti-freezing agent, wetting agents, anti-foaming agents, colorants, pigments, binder/structuring agents, biocides/anti-microbial agent, thickeners, emulsifiers, buffering agent, fillers, diluents/solvents, quick coating agents or sticking agents, preservatives, adjuvants and solvents or a combination thereof.
In another embodiment of the present invention, thickeners are selected from the group comprising silicates such metal silicates, aluminum magnesium silicate sodium carboxy methyl cellulose, methyl cellulose, ethyl cellulose, poly vinyl alcohol, sodium alginate, sodium poly acrylate, xanthan gum, welan gum, gum arabic, montmorillonite, lingo sulfonates, hydroxy methyl cellulose, dextrin, starch, Bentonite Clay, Polysaccharides or combinations thereof.
In another embodiment of the present invention, the anti-freezing agent is selected from the group comprising polyethylene glycols, methoxy polyethylene glycols, propylene glycol, polypropylene glycols, polybutylene glycols, Mono ethylene glycol, glycerin, ethylene glycol, Glycerin, Diethylene glycol or combinations thereof.
In another embodiment of the present invention, the anti-foaming agent is selected from the group comprising silicone oils, or mineral oils, Fatty acid ester; potassium sorbate, and parahydroxy benzoates, silicon emulsion based anti-foam agents, Siloxane polyalkylene oxide, Polydimethyl Siloxane, tri siloxane ethoxylates or combinations thereof.
In another embodiment of the present invention, the wetting agent is selected from the group comprising Tristyrylphenol ethoxylate nonionic emulsifier, Mixture of non-ionic surfactants & Alkoxylated Alcohol/Block copolymer, sodium lauryl sulphate; sodium dioctyl sulphosuccinate; Tristyrylphenol ethoxylate non-ionic emulsifier/ mixture of non-ionic surfactants and alkoxylated Alcohol/Block copolymer, ionic surfactant mixtures, anionic

surfactants, alkyl phenol ethoxylates, aliphatic alcohol ethoxylates and the salts or combinations thereof.
In yet another embodiment of the present invention, the polymers are selected from the group comprising polyvinyl alcohols, polyvinylpyrrolidone, gel-forming carraagheenans, water-soluble gelatine and casein, superabsorbent polymers such polyacrylamides and polyacrylates based polymers, semi-synthetic or fully-synthetic peptide/protein-based superabsorbent polymers such as collagen-based synthetic polymers, elastin-like polypeptides, polyaspartic acid, polyaspartates, polyglutamic acid, polyglutamate, Semi-synthetic or fully-synthetic polysaccharide: carboxymethyl starch , sulfoethyl starch, carboxymethyl cellulose, sulfoethyl cellulos, hydroxypropyl cellulose, hydroxyethyl cellulose, methylcellulose, chitosan, acrylonitrile, acrylic acid, methacrylic acid, acrylamide, methacrylamide, 2-acrylamido-2-methyl- propanesulfonic acid (AMPS), vinyl sulfonic acid, ethyl acrylate, and potassium acrylate or combinations thereof.
In another embodiment of the present invention, adjuvant is selected from the group comprising Silicone Ethoxylated Oil, Polyvinyl Pyrrolidon,Poly vinyl Alcohol,Blend of poly terpene resin or combination thereof.
In another embodiment of the present invention, diluent/solvents are selected from the group comprising lactose, glucose, fructose, maltose, sucrose, in the anhydrous or hydrate forms, urea, water soluble or dispersible polymers, water soluble inorganic salts, Demineralized (DM) water, Vegetable Oil or combination thereof.
In another embodiment of the present invention, filler is selected from the group comprising Silicon Dioxide, China -Clay, Kaolin, Talc, starch or combination thereof.
In another embodiment of the present invention, the biocide/anti-microbial agent is selected from the group comprising Benzisothiazolin-3-one / Formaldehyde/Sodium benzoate/Sodium o-phenylphenate, 5-chloro-2-methyl-4-isothiazolin-3-one & 2-methyl-4-isothiazolin-3-one or combination thereof.
In another embodiment of the present invention, the composition is in form selected from Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for

dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable Slurry (FS), Flowable Suspension(FS), Suspension Concentrate (SC), Suspension concentrate for direct application (SD), Suspo- emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Water soluble powder (SP), Water dispersible powder for slurry seed treatment (WS), Water dispersible granules (WDG), Wettable powders (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), granular (GR), oil in dispersion (OD), a mixed formulation of CS and SC (ZC) or a mixed formulation of CS and SE (ZE), a mixed formulation of CS and EW (ZW).
In another embodiment of the present invention provides a method for controlling a plant disease caused by a fungal plant pathogens comprising applying to the plant or portion thereof or soil, a fungicidally effective amount of the present invention fungicidal composition.
In another embodiment of the present invention provides a method of improving vigour or yield of the plant comprising, treating a plant or portion thereof or soil, a fungicidally effective amount of the fungicidal composition.
In another embodiment of the present invention, the plant is selected from the group consisting of cereals, field crops, fruits, seeds, tree nuts, vegetables, turf grasses, potatoes, tomatoes, grapes and ornamentals.
In yet another embodiment of the present invention, it provides a process for preparing a stable fungicidal composition comprising of as active ingredients, (a) Valifenalate or its agrochemically acceptable salts and (b) at least one fungicide selected from Strobilurin fungicides or their agrochemically acceptable salts.
In one embodiment, the compositions according to the present invention acts synergistically to control pests in various crops.
Inventors of the present invention succeeded in providing stable and efficacious synergistic composition comprising synergistic and bio-effective amount of acylamino acid fungicide and at least one compound selected from strobilurin fungicide. The following illustrations of

examples are intended to illustrate a stable synergistic fungicidal composition, but in no way limit the scope of the present invention.
Examples
Having described the basic aspects of the present invention, the following non-limiting examples illustrate specific embodiment thereof. Those skilled in the art will appreciate that many modifications may be made in the invention without changing the essence of invention. The composition and process of present invention is exemplified by the following non-limiting examples.
Example 1:
A stable fungicidal composition comprising Valifenalate and Trifioxystrobin as active ingredients, according to the present invention was prepared as follows:
Table 1

Fungicidal Composition
Sr. No. Ingredients of composition % w/w
1 Valifenalate Technical 1-80%
2 Trifioxystrobin Technical 1-40%
3 Dispersing Agent- Polyarylphenyl ether phosphate, tristyryl phenol ethylated /Acrylic graft
Copolymer/ Ethoxylated Tristryl phenol Sulphate,Naphthalene sulfonic acid,sodium salt condensate with formaldehyde,Ethoxylated oleyl cetyl alcohol, Polyalkelene glycol ether,Ethoxylated Fatty alcohol 0.5-10%
4 Wetting Agent- Tristyryl phenol ethoxylate nonionic emulsifier/ Mixture of non-ionic surfactants & Alkoxyleted Alcohol/Block copolymer 0.5-10%
5 Antifoaming Agent-Siloxane polyalkyleneoxide 0.01-1%
6 Antifreezing Agent- Glycol,Propylene Glycol,Mono ethylene glycol,Glycerin,Diethylene glycol 0.1-10%
7 Adjuants-Silicone Ethoxylated Oil, Polyvinyl Pyrrolidon,Poly vinyl Alcohol,Blend of poly terpene resin 0.1-10%

8 Filler- Silicon Dioxide/China -Clay/Kaolin/Talc/starch 0.1-5%
9 Anti-bacterial/Biocide- Benzisothiazolin-3-one / Formaldehyde/Sodium benzoate/Sodium o-phenylphenate, 5-chloro-2-methyl-4-isothiazolin-3-one & 2-methyl-4-isothiazolin-3-one 0.01-1%
10 Thickeners: Polysaccharides/carboxymethyl cellulose/Bentonite Clay/Aluminum Magnesium Silicate 0.01-3%
11 Solvent: DM water/Vegetable Oil Q.s to make 100
Total 100
A stable Suspension Concentrate (SC) of Valifenalate and Trifloxystrobin according to the present invention was prepared as follows:
Table 2

Suspension Concentrate (SC) formulation
Sr. No. Ingredients of composition % w/w
1 Valifenalate Technical 20.00%
2 Trifloxystrobin Technical 10.00%
3 Dispersing Agent- Tristyrylphenol Ethoxylate Amine salt of phosphate 3%
4 Wetting Agent- Block copolymer, 2%
5 Antifoaming Agent-Siloxane polyalkyleneoxide 0.20%
6 Antifreezing Agent- Propylene Glycol 5%
7 Adjuvant-Silicone ethoxylated Oil 1%
8 Filler- China -Clay 1%

9 Anti-bacterial - Benzisothiazolin-3-one 0.10%
10 Polysaccharides 0.10%
11 DM water Q.sto
make
100
Total 100
Table 3: SC Formulations

SC (Suspension Concentrate) formulation
Sr. No. Component Composition

1 2 3 4 5
1 Valifenalate Technical 50 2 10 3 14
2 Trifloxystrobin Technical 1 40 30 2 20
3 Acrylic graft Copolymer 3.00 3.00 3.00 3.00 3.00
4 Naphthalene sulfonic acid,sodium salt condensate with formaldehyde 4.00 4.00 4.00 4.00 4.00
5 Ethoxylated Silicone oil 1.00 1.00 1.00 1.00 1.00
6 Silicone Antifoam 0.50 0.50 0.50 0.50 0.50
7 Benzisothiazoline 0.10 0.10 0.10 0.10 0.10
8 Glycol 5.00 5.00 5.00 5.00 5.00
9 Polysaccharides 0.10 0.10 0.10 0.10 0.10
10 DM water QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100
Process: Required quantity of water, biocide, defoamer and homogenise followed by slow addition of gum powder with stirring to obtain a gum solution (Gum Solution should be made 12-18 hour prior to use). Required quantity of DM water, wetting agent, dispersing agent and suspending agents, colourant/dye was added into the charged vessel followed by homogenization for about between 45 - 60 minutes using high shear homogeniser to obtain a

homogenized slurry. Technical and other remaining adjuvants excluding 'antifreeze & thickeners' were added into the homogenized slurry to obtain a uniform slurry. Half of the quantity of required antifoam agent was added into the slurry. The uniform slurry mixture was then passed through appropriate particle size reduction equipment (Dyno-Mill) until the granule material of the desired particle size was achieved. Remaining half of the quantity of required antifoam agent along with the antifreeze agent was added to the granule material as obtained. Gum solution as obtained in the first step was then added to obtain the Suspension Concentrate formulation.
Example 2:
A stable Water Dispersible Granule (WG) formulation comprising Valifenalate and Trifloxystrobin as active ingredients according to the present invention was prepared as follows:
Table 4:

WG (Water Dispersible Granule) formulation
Sr. No. Component % w/w
1 Valifenalate Technical 30.00%
2 Trifloxystrobin Technical 10.00%
3 Dispersing Agent-Sodium Polycarboxylate 3.00
4 Wetting Agent-Sodium Lauryl Sulfate 4.00
5 Dispersing Sodium alkyl naphthalene sulfonate blend 1.00
6 Adjuvants-Poly vinyl pyrolliddone 0.10
7 Antifoam-Polydimethyl Siloxane 0.10
8 Filler-China Clay QS to Make 100
Table 5: WG Formulations

WG (Water Dispersible Granule) formulation
Sr. No. Component Composition

1 2 3 4 5

1 Valifenalate Technical 80 1 3 20 30
2 Trifloxystrobin Technical 1 40 5 25 20
3 Sodium Polycarboxylate 8.00 11.00 10.00 1.00 10.00
4 Sodium Lauryl Sulfate 6.00 7.00 4.00 5.00 4.00
5 Sodium ligno sulfonate 2.00 1.00 1.00 1.00 1.00
6 Sodium
alkylnaphthalenesulfonate, formaldehyde condensate 0.50 0.50 0.50 0.50 0.50
7 Silicone based antifoam 0.10 0.10 0.10 0.10 0.10
8 Ethoxylated Silicone oil 0.10 0.10 0.10 0.10 0.10
9 Precipitated Silica 0.10 0.10 0.10 0.10 0.10
10 China Clay QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100
Process: Required quantity of filler, wetting agent, dispersing agent, and suspending agent, and technical was mixed in a premixing blender for homogenization for a period of 30 minutes to obtain a pre-blended material. The pre-blended material as obtained in the first step was grinded through Jet mill/ air classifier mills followed by blending in post blender for a period of -1.5 hour to obtain a homogeneous mixture. Homogeneous material is analysed, and material is unloaded into 25 kg HDPE bag with LDPE liner inside. Required quantity of water (qs) was then added to make a dough. The dough was then passed through the extruder to obtain granules of required size. Wet granules as obtained were passed through the fluidised bed drier followed by grading using vibrating screens to obtain the wettable granules. Obtained Wettable granules are analysed to quality check and packed.
Example 3:
A stable Wettable Powder (WP) formulation comprising Valifenalate and Trifloxystrobin as active ingredients according to the present invention was prepared as follows:
Table 6:
WP (Wettable Powder) formulation

Sr. No.

Component

% w/w


1 Valifenalate Technical 50.00%
2 Trifloxystrobin Technical 30.00%
3 Dispersing Agent-Sodium ligno sulfonate 3.00
4 Wetting Agent-Sodium Lauryl Sulfate 4.00
5 Dispersing Sodium alkyl naphthalene sulfonate blend 1.00
6 Adjuvants-Poly vinyl pyrolliddone 1.00
7 Antifoam-Polydimethyl Siloxane 0.10
8 Filler-China Clay QS to Make 100
Table 7: WP Formulations

WP (Wettable Powder) formulation
Sr. No. Component Composition

1 2 3 4 5
1 Valifenalate Technical 80 1 3 20 30
2 Trifloxystrobin Technical 1 40 5 25 20
3 Sodium Polycarboxylate 8.00 8.00 8.00 8.00 8.00
4 Sodium Lauryl Sulfate 5.00 5.00 5.00 5.00 5.00
5 Blend of poly terpene resin 1.00 1.00 1.00 1.00 1.00
6 Sodium
alkylnaphthalenesulfonate, formaldehyde condensate 0.50 0.50 0.50 0.50 0.50
7 Silicone based antifoam 0.10 0.10 0.10 0.10 0.10
8 Starch 3.00 5.00 5.00 5.00 5.00
9 Precipitated Silica 0.10 0.10 0.10 0.10 0.10
10 China Clay QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100
Process: Required quantity of filler, wetting agent, dispersing agent, suspending agent, and technical were charged in premixing blender for homogenization for about 30 minutes to obtain

a pre-blended material. The pre-blended material was grinded through Jet mill/ air classifier mills followed by blending in post blender for a period of -1.5 hr to obtain a homogeneous material. The homogenous material as obtained was analysed and unloaded.
Example 4:
A stable Flowable concentrate for seed treatment (FS) of Valifenalate and Trifloxystrobin according to the present invention was prepared as follows:
Table 8

Flowable Slurry(FS) formulation
Sr. No. Ingredients % w/w
1 Valifenalate Technical 20.00%
2 Trifloxystrobin Technical 10.00%
3 Dispersing Agent- Tristyrylphenol Ethoxylate Amine salt of phosphate 3%
4 Wetting Agent- Block copolymer, 2%
5 Antifoaming Agent-Siloxane polyalkyleneoxide 0.20%
6 Antifreezing Agent- Propylene Glycol 5%
7 Filler- China -Clay 1%
8 Anti-bacterial - Benzisothiazolin-3-one 0.10%
9 Adjuants-Silicone ethoxylated Oil 1%
10 Polysaccharides 0.10%
11 DM water Q.sto make 100
Total 100
Table 9: FS Formulation

FS (Flowable Slurry) formulation
Sr. No. Component Composition

1 2 3 4 5
1 Valifenalate Technical 40 2 10 3 14

2 Trifloxystrobin Technical 1 40 30 2 20
3 Acrylic Copolymer 3.00 3.00 3.00 3.00 3.00
4 Naphthalene sulfonic acid,sodium salt condensate with formaldehyde 4.00 4.00 4.00 4.00 4.00
5 Ethoxylated Silicone oil 1.00 1.00 1.00 1.00 1.00
6 Silicone Antifoam 0.50 0.50 0.50 0.50 0.50
7 Benzisothiazoline 0.10 0.10 0.10 0.10 0.10
8 Glycol 5.00 5.00 5.00 5.00 5.00
9 Polysaccharides 0.10 0.10 0.10 0.10 0.10
10 DM water QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100
Process: Required quantity of water, biocide, and defoamer followed by addition of gum powder are homogenized with stirring to obtain a gum solution (Gum Solution should be made 12-18 hour prior to use). Required quantity of DM water, wetting agent, dispersing agent & suspending agents, colourant/dye was added into the charged vessel followed by homogenization for a period of ranging between 45 - 60 minutes using high shear homogeniser to obtain a homogenized slurry. Technical and other remaining adjuvants excluding 'antifreeze & thickeners' were added into the homogenized slurry to obtain a uniform slurry. Half of the quantity of required antifoam agent was added into the slurry. The uniform slurry mixture was then passed through appropriate particle size reduction equipment (Dyno-Mill) until the granule material of the desired particle size was achieved. Remaining half of the quantity of required antifoam agent along with the antifreeze agent was added to the granule material as obtained. Gum solution as obtained in the first step was then added to obtain the Flowable concentrate for seed treatment (FS).
Example 5: A stable Suspo Emulsion(SE) formulation comprising Valifenalate and Trifloxystrobin as active ingredients according to the present invention was prepared as follows:

Table 10

Suspo Emulsion(SE) formulation
Sr. No. Ingredients % w/w
1 Valifenalate Technical 15.00%
2 Trifloxystrobin Technical 20.00%
3 Dispersing Agent- Tristyrylphenol Ethoxylate Amine salt of phosphate 3%
4 Wetting Agent- Block copolymer, 2%
5 Antifoaming Agent-Siloxane polyalkyleneoxide 0.20%
6 Antifreezing Agent- Propylene Glycol 5%
7 1-octanol 10%
8 Anti-bacterial - Benzisothiazolin-3-one 0.10%
9 Adjuvants-Silicone ethoxylated Oil 1%
10 Polysaccharides 0.10%
11 DM water Q.stomake 100
Total 100
Table 11: Suspo Emulsion Formulation

SE (Suspo Emulsion) formulation
Sr. No. Component Composition

1 2 3 4 5
1 Valifenalate Technical 50 2 10 3 14
2 Trifloxystrobin Technical 1 40 30 2 20
3 Acrylic Copolymer 3.00 3.00 3.00 3.00 3.00
4 Naphthalene sulfonic acid,sodium salt condensate with formaldehyde 4.00 4.00 4.00 4.00 4.00
5 Ethoxylated Silicone oil 1.00 1.00 1.00 1.00 1.00
6 Silicone Antifoam 0.50 0.50 0.50 0.50 0.50
7 Benzisothiazoline 0.10 0.10 0.10 0.10 0.10
8 Glycol 5.00 5.00 5.00 5.00 5.00
9 1-Octanol 30.00 25.00 20.00 30.00 15.00

10 Polysaccharides 0.10 0.10 0.10 0.10 0.10
QSto QSto QSto QSto QSto
11 DM water Make Make Make Make Make
100 100 100 100 100
Process: Gum Solution should be prepared 12-18 hours before use. Required amount of water, biocide, defoamer were homogenised, followed by slow addition of the gum powder and stir until completely dissolved. Charge the required amount of DM water in the designated vessel for production. Add the required amount of wetting agent, dispersing agent, and suspending agents, as well as the colorant/dye, and homogenise the contents for 45-60 minutes with a high shear homogeniser. Followed by addition of technical and other remaining adjuvants, excluding 'antifreeze and thickeners,' and homogenised to obtain a uniform slurry ready for grinding. Before grinding, half the amount of antifoam was added, and the material was grinded three times in the Dyno mill. Further, half quantity of antifoam was added along with antifreeze after grinding process, and before sampling for in process analysis. Finally, gum solution added to obtain final formulation, after quality check.
Example 6: A stable Granule (GR) formulation comprising Valifenalate and Trifloxystrobin, as active ingredients according to the present invention was prepared as follows:
Table 12

Granule(GR) formulation
Sr. No. Ingredients % w/w
1 Valifenalate Technical 2.00%
2 Trifloxystrobin Technical 2.00%
3 Dispersing Agent- Calcium Ligno sulphate 3%
4 Pigment Blue 0.20%
5 Filler- China -Clay 5%
6 adjuvants-Poly vinyl pyrolliddone 0.10%
7 DM water 1%
8 Sand Q.sto make 100
Total 100

Table 13: GR Formulation

GR (Granule)
Sr. No. Component Composition

1 2 3 4 5
1 Valifenalate Technical 10 11 12 15 10
2 Trifloxystrobin Technical 3 5 5 5 5
3 Sodium Polycarboxylate 3.00 3.00 3.00 3.00 3.00
4 Sodium Lauryl Sulfate 4.00 4.00 4.00 4.00 4.00
5 Pigment blue 0.10 0.10 0.10 0.10 0.10
6 Blend of poly terpene resin 0.50 0.50 0.50 0.50 0.50
7 China Clay 5.00 5.00 5.00 5.00 5.00
8 DM water 1.00 2.00 1.00 2.00 1.00
9 Sand QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100 QSto
Make
100
Process: Charge the required amount of filler, wetting agent, dispersing agent, suspending agent, and technical in premixing blender for30 minutes to homogenise. The pre-blended material is than grinded through Jet mill/ air classifier mills. Finely grinded material is blended in post blender till it becomes homogeneous (for approx. 1.5 hr). Homogeneous material is analysed. After getting approval from QC dept. material is unloaded into 25 kg. FIDPE bag with LDPE liner inside. Charge the required quantity of sand in granulator. Add DM water, sticking agent and remaining material till it become homogeneous. Then add finely grinded material to completely coated on sand. Now blend this formulation for 30 Minutes. Final product is sent for QC approval. After approval material is packed in required pack sizes.
Example 7: A stable OD (Oil Dispersion) formulation comprising Valifenalate and Trifloxystrobin as active ingredients, according to the present invention was prepared as follows:

Table 14

OD(Oil Dispersion) formulation
Sr. No. Ingredients % w/w
1 Valifenalate Technical 5.00%
2 Trifloxystrobin Technical 25.00%
3 Dispersing Agent- Tristyrylphenol Ethoxylate Amine salt of phosphate 3%
4 Dispersing Agent Ethoxylated oleyl cetyl alcohol 3.00
5 Sticking Agent-Polyvinylpyrrolidone 1.00
6 Emulsifier-Calcium alkyl benzen sulfonate 5.00
7 Emulsifier-Castor oil ethoxylates 4.00
8 Antifoam-Polydimethyl Siloxane 0.00
9 Antifreezing Agent- Propylene Glycol 0.00
10 Vegetable Oil-Soyabean Oil Q.sto make 100
Total 100
Table 15: OD formulation

OD(Oil Dispersion)
Sr. No. Component Composition

1 2 3 4 5
1 Valifenalate Technical 20 2 10 3 14
2 Trifloxystrobin Technical 1 30 15 2 10
3 Ethoxylated oleyl cetyl alcohol 3.00 3.00 3.00 3.00 3.00
4 Polyalkelene glycol ether 4.00 4.00 4.00 4.00 4.00
5 Polyvinylpyrrolidone 1.00 1.00 1.00 1.00 1.00
6 Calcium alkyl benzen sulfonate 3.00 3.00 3.00 3.00 3.00
7 Castor oil ethoxylates 2.00 2.00 2.00 2.00 2.00
8 Silicone based antifoam 0.10 0.10 0.10 0.10 0.10
9 Propylene glycol 5.00 5.00 5.00 5.00 5.00

QSto QSto QSto QSto QSto
10 Soyabean Oil Make Make Make Make Make
100 100 100 100 100
Process: Charge required quantity of vegetable oil in vessel and add polyvinyl pyrrollidone, mix well for 30 minutes using high shear homogenizer. Followed by addition of required quantity of Wetting agent, dispersing agent, suspending agents and homogenise the contents for 45 - 60 minutes using high shear homogeniser. Subsequently, technical was added and homogenized for 30 minutes, and passed the homogenised material through horizontal bead mill to get required particle size. After completion of grinding cycles sample is sent to QC for A.I. & particle size analysis, after approval from QC, material is transferred to storage tank till packing.
The fungicide composition of the present invention is particularly useful as an agricultural and horticultural fungicide. Target diseases of the agricultural and horticultural fungicide, for example, include rice blast (Magnaporthe grisea), brown spot (Cochliobolus miyabeanus), sheath blight (Rhizoctonia solani), Bakanae disease (Gibberella fujikuroi), damping-off (Fusarium spp., Rhizopus spp., Pythium spp.) of rice; powdery mildew (Blumeria graminis), mottle leaf (Pyrenophora graminea), net blotch (Pyrenophora teres), Fusarium blight (Fusarium graminearum, Microdochium nivale), brown rust (Puccinia recondita), browning root rot (Pythium iwayamai), loose smut (Ustilago nuda), ergot (Clavicepspurpurea), eye spot (Pseudocercosporella herpotrichoides), scald (Rhynchosporium secalis), speckled leaf blotch (Septoria tritici), glume blotch (Septoria nodorum), Typhulabvown snow blight (Typhula incarnatd), Typhula black snow blight (Typhula ishikariensis), Sclerotinia snow blight (Sclerotinia borealis), pink snow mold (Microdochium nivale), damping-off (Gaeumannomyces graminis) of wheat and barley; brown spot (Cochliobolus heterostrophus), rust (Puccinia sorghi), northern leaf blight (Setosphaeria turcica), leaf spot (Physoderma maydis), smut (Ustilago maydis) of maize; rust (Puccinia melanocephala), smut (Ustilago scitaminea), top rot (Gibberella fujikuroi), leaf blight (Pseudocercospora taiwanensis) of sugar cane; black spot (Diaporthe citri), scab (Elsinoe fawcettii), gray mold (Botrytis cinerea) of citruses; blossom blight (Monilinia mali), apple canker (Valsa ceratosperma), powdery mildew (Podosphaera leucotricha), Alternaria blotch (Alternaria alternata apple pathotype), black spot (Venturia inaequalis), anthracnose (Colletotrichum acutatum, Glomerella cingulata) of apple; black spot (Venturia nashicola), Alternaria leaf spot

(Alternaria alternata Japanese pear pathotype), chocolate spot (Gymnosporangium asiaticum),
late blight (Phytophthora cactorum), anthracnose (Colletotrichum acutatum, Glomerella
cingulata), ring spot (Botryosphaeria berengeriana), powdery mildew (Phyllactinia mali) of
pears; brown rot (Monilinia fructicold), black spot (Cladosporium
carpophilum), Phomopsis rot (Phomopsis sp.), bacterial shot hole (Leptosphaeria michotii) of
peach; downy mildew (Plasmopara viticola), bird's eye rot (Elsinoe ampelina), ripe rot
(Glomerella cingulata), powdery mildew (Uncinula necator), rust (Physppella ampelopsidis),
gray mold (Botrytis cinered) of grapes; anthracnose (Glomerella cingulata), anguler leaf spot
(Cercospora kaki), circular leaf spot (Mycosphaerella nawae), gray mold (Botrytis cinered),
powdery mildew (Phyllactinia kakicola), late blight (Phytophthora citrophthora) of
persimmon; powdery mildew (Sphaerotheca fuliginea), late blight (Phytophthora cryptogea),
brown rot (Phytophthora capsici), Sclerotinia rot (Sclerotinia sclerotiorum), damping-off
(Pythium debaryanum), gummy stem blight (Didymella bryoniae), Fusarium wilt (Fusarium
oxysporum), Verticillium wilt (Verticillium dahliae), downy mildew (Pseudoperonospora
cubensis) of watermelon; powdery mildew (Erysiphe polygoni, Sphaerotheca cucurbitae), late
blight (Phytophthora melonis), Corynespora leaf spot (Corynespora
cassiicola), Sclerotinia rot (Sclerotinia sclerotiorum), anthracnose (Colletotrichum orbiculare), gummy stem blight (Didymella bryoniae), Fusarium wilt (Fusarium oxysporum), damping-off (Pythium cucurbitacearum), gray mold (Botrytis cinered), Verticillium wilt (Verticillium dahliae), downy mildew (Pseudoperonospora cubensis) of cucumber; powdery mildew (Sphaerothecafuliginea), late blight (Phytophthora nicotianae), Corynespora leaf spot (Corynespora cassiicola), Sclerotinia rot (Sclerotinia sclerotiorum), gummy stem blight (Didymella bryoniae), Fusarium wilt (Fusarium oxysporum), damping-off (Pythium spinosum), gray mold (Botrytis cinered), Verticillium wilt (Verticillium dahliae), downy mildew (Pseudoperonospora cubensis) of melon; powdery mildew (Sphaerotheca cucurbitae), late blight (Phytophthora capsici), Sclerotinia rot (Sclerotinia sclerotiorum), gummy stem blight (Didymella bryoniae), downy mildew (Pseudoperonospora cubensis) of pumpkin; Fusarium wilt (Fusarium oxysporum), gray mold (Botrytis cinered), Sclerotinia rot (Sclerotinia sclerotiorum), southern blight (Sclerotium rolfsii), late blight (Phytophthora infestans), brown rot (Phytophthora capsici), brown spot (Phomopsis vexans), powdery mildew (Erysiphe cichoracerum, Oidiopsis sicula) of eggplant; late blight (Phytohthora infestans), early blight (Alternaria solani), black mole disease (Thanatephorus cucumeris), scab (Streptomyces spp.), bacterial soft rot (Pectobacterium carotovorum), powdery scab (Spongospora subterranean) of potato; late blight (Phytophthora infestans), fruit rot

(Phytophthora capsici), early blight (Alternaria solani), leaf mold (Fulvia fulva), powdery mildew (Oidium lycopersici), brown rot (Phytophthora nicotianae), anthracnose (Colletotrichum gloeosporioides), damping-off (Pythium vexans, Rhizoctonia solani) of tomato; late blight (Phytophthora capsici), powdery mildew (Oidiopsis sicula), seedling damping-off (Rhizoctonia solani), leaf spot (Cercospora capsici) of sweet pepper; late blight (Phytophthora sp.), downy mildew (Peronospora effusa), damping-off (Pythium aphanidermatum), Fusarium wilt (Fusarium oxysporum), stock rot (Rhizoctonia solani), root rot (Aphanomyces cochlioides) of spinach; white late blight (Phytophthora porri), late blight (Phytophthora nicotianae), southern blight (Sclerotium rolfsii), damping-off (Rhizoctonia solani), Alternaria leaf spot (Alternariaporri), soft rot (Pectobacterium carotovorum), downy mildew (Peronospora destructor), rust (Puccinia allii) of green onions; Fusarium basal rot (Fusarium oxysporum), small rot (Ciborinia alii), gray mold (Botrytis cinerea), gray rot (Botrytis allii) of onion; downy mildew (Peronospora parasitica), clubroot (Plasmodiophora brassicae), white rust (Albugo macrospora), Alternaria leaf spot (Alternaria brassicae), white spot (Cercosporella brassicae), Sclerotinia rot (Sclerotinia sclerotiorum), verticillium wilt (Verticillium dahliae), bottom rot (Rhizoctonia solani), foot rot (Aphanomyces raphani), Pythium rot (Pythium ultimum) of cruciferous vegetables; Sclerotinia rot (Sclerotinia sclerotiorum), rust (Phakopsora pachyrhizi), southern blight (Sclerotium rolfsii), damping-off (Fusarium oxysporum), anthracnose (Colletotrichum truncatum, C. trifolii, Glomerella glycines, Gloeosporium sp.), gray mold (Botrytis cinerea) of pulses; downy mildew (Peronospora manshurica), stem blight (Phytophthora sojae), purple speck (Cercospora kikuchii), Sphaceloma scad (Elsinoe glycines), pod and stem blight (Diaporthe phaseolorum) of soybean; leaf spot (Mycosphaerella personata), brown leaf spot (Mycosphaerella arachidis) of peanuts; powdery mildew (Sphaerotheca aphanis), Fusarium wilt (Fusarium oxysporum), Verticillium wilt (Verticillium dahliae), late blight (Phytophthora cactorum), Alternaria leaf spot (Alternaria alternata), leaf spot (Mycosphaerella fragariae), anthracnose (Colletotrichum acutatum, Glomerella cingulata), gray mold (Botrytis cinerea), bud blight (Rhizoctonia solani) of strawberry; stem rot (Sclerotinia sclerotiorum), bottom rot (Rhizoctonia solani), damping-off (Pythium sp.), root rot (Fusarium oxysporum), gray mold (Botrytis cinerea), downy mildew (Bremia lactucae) of lettuce; Fusarium wilt (Fusarium oxysporum), powdery mildew (Erysiphe heraclei), black leaf blight (Alternaria dauci), stain rot (Pythium sulcatum), root rot (Rhizoctonia solani), leaf spot (Cercospora carotae) of carrot; net blister blight (Exobasidium reticulatum), white scab (Elsinoe leucospila), brown round spot (Pseudocercospora ocellata, Cerocospora chaae),

blister blight (Exobasidium vexans), ring spot (Pestaltiopsis longiseta), anthracnose (Discula theae-sinensis) of tea; brown spot (Alternaria alternatd), powdery mildew (Erysiphe cichoracearum), anthracnose (Colletotrichum cichoracearum) of tabacco; Cercospora leaf spot (Cercospora beticola) of sugar beet; black spot (Diplocarpon rosae), powdery mildew (Sphaerotheca pannosa), downy mildew (Peronospora sparsa), late blight (Phytophthora megasperma) of rose; powdery mildew (Erysiphe cichoracearum), late blight (Phytophthora cryptogea), Sclerotinia rot (Sclerotinia sclerotiorum), Alternaria leaf spot (Alternaria helianthi), downy mildew (Plasmopara halstedii), brown spot (Septoria helianthi) of sunflower; Fairy ring disease (Bovista dermoxantha, Lycoperdon pusillum, Conocybe lactea, Vascellum curtisii), pseudo-leaf rot (Ceratobasidium spp.), Curvularia leaf blight (Curvularia geniculata), leaf rot (Rhizoctonia solani), pink snow mold (Microdochium nivale), Pythium disease (Pythium spp.), rust (Puccinia spp.), dollar spot (Sclerotinia homoeocarpa), Pythium shoot blight (Pythium aphanidermatum), browning root rot (Pythium iwayamai), Typhula snow blight (Typhula incarnata, T. isikariensis), anthracnose (Colletotrichum sp.) of turf grass, and the like.
The ingredient (a) and the ingredient (b) constituting the fungicide composition of the present invention may be mixed with various adjuvants in the same manner as conventional agricultural chemicals, and used in the form of a formulation such as a dust, granules, water-dispersible granules, a wettable powder, a water-based suspension concentrate, an oil-based suspension concentrate, water-soluble granules, an emulsifiable concentrate, a soluble concentrate, a paste, an aerosol, or an ultra-low-volume formulation. However, so long as it is suitable for the purpose of the present invention, it may be formulated into any type of formulation which is commonly used in this field. Such adjuvants to be used in the formulation include solid carriers such as diatomaceous earth, slaked lime, calcium carbonate, talc, white carbon, kaolin, bentonite, a mixture of kaolinite and sericite, clay, sodium carbonate, sodium bicarbonate, mirabilite, zeolite, and starch; solvents such as water, toluene, xylene, solvent naphtha, dioxane, acetone, isophorone, methyl isobutyl ketone, chlorobenzene, cyclohexane, dimethyl sulfoxide, dimethylformamide, dimethylacetamide, N-methyl-2-pyrrolidone, and alcohols; anionic surfactants and spreaders such as a salt of fatty acid, a benzoate salt, an alkylsulfosuccinate salt, a dialkylsulfosuccinate salt, a polycarboxylate salt, a salt of alkylsulfuric acid ester, an alkyl sulfate salt, an alkylaryl sulfate salt, an alkyl diglycol ether sulfate salt, a salt of alcohol sulfuric acid ester, an alkyl sulfonate salt, an alkylaryl sulfonate salt, an aryl sulfonate salt, a lignin sulfonate salt, an alkyldiphenyl ether disulfonate salt, a

polystyrene sulfonate salt, a salt of alkyl phosphoric acid ester, an alkylaryl phosphate salt, a styrylaryl phosphate salt, a salt of polyoxyethylene alkyl ether sulfuric acid ester, a polyoxyethylene alkylaryl ether sulfate salt, a salt of polyoxyethylene alkylaryl ether sulfuric acid ester, a polyoxyethylene alkyl ether phosphate salt, a salt of polyoxyethylene alkylaryl phosphoric acid ester, and a salt of a condensate of naphthalene sulfonate-formalin; nonionic surfactants and spreaders such as a sorbitan fatty acid ester, a glycerin fatty acid ester, a fatty acid polyglyceride, a fatty acid alcohol polyglycol ether, acetylene glycol, acetylene alcohol, an oxyalkylene block polymer, a polyoxyethylene alkyl ether, a polyoxyethylene alkylaryl ether, a polyoxyethylene styrylaryl ether, a polyoxyethylene glycol alkyl ether, a polyoxyethylene fatty acid ester, a polyoxyethylene sorbitan fatty acid ester, a polyoxyethylene glycerin fatty acid ester, a polyoxyethylene hydrogenated castor oil, and a polyoxypropylene fatty acid ester; vegetable and mineral oils such as olive oil, kapok oil, castor oil, palm oil, camellia oil, coconut oil, sesame oil, corn oil, rice bran oil, peanut oil, cottonseed oil, soybean oil, rapeseed oil, linseed oil, tung oil, and liquid paraffin; and the like. Such adjuvants may be selected for use among those known in this field, so long as such selection does not depart from the purpose of the present invention. Further, it is possible to use commonly employed various adjuvants such as a filler, a thickener, an anti-settling agent, an anti-freezing agent, a dispersion stabilizer, a phytotoxicity reducing agent, an anti-mold agent, etc. In actual use of such a formulation, it may be used as it is, or after it is diluted with a diluting agent such as water to a predetermined concentration and, as a case requires, various spreaders are added.
Additionally, the fungicide composition of the present invention may be used in combination with other agricultural chemicals, for example, a fungicide, an insecticide, a miticide, a nematicide, a soil pesticide, an antivirus agent, an attractant, a herbicide, and a plant growth regulating agent. In this case, a further excellent effect may be exhibited.
BIO-EFFICACY DATA:
Evaluation of synergistic effect of pesticidal composition of the present invention
A synergistic effect of two or more products exists whenever the action of an active ingredient combination is greater than the sum of the actions of the individual components. Synergism was calculated by using Colby's method (Weeds, vol. 15 No. 1 (Jan 1967), pp. 20-2.
The synergistic action expected for a given combination of two active components can be calculated as follows:
XY

E = (X + Y)-
100
Where:
E represents expected percentage of pesticidal control for the combination of the two or three
active ingredients at defined doses (for example equal to x, y and z, respectively).
X is the percentage of pesticidal control observed by the compound (Trifloxystrobin in present case) at a defined dose (equal to x).
Y is the percentage of pesticidal control observed by the compound (Valifenalate in present case) at a defined dose (equal to y).
When the percentage of pesticidal control observed for the combination is greater than the
expected percentage, there is a synergism effect.
Observed control (%)
Ratio =
Expected control (%)
Ratio of O/E > 1, synergism observed
The present invention is illustrated by way of examples, the examples are meant for illustrative purposes and should not be construed as limiting.
Experiment for synergistic activity of composition of present invention:
Example 8-
Details of experiment on tomato crop:
Field experiment for synergistic activity of Trifloxystrobin and Valifenalate for the control of early blight (caused by Alternaria solan?) and late blight (caused by Phytophthora infestans) diseases in tomato crop was conducted at farmer field, Sonipat (Haryana).
To evaluate the synergistic effect, tomato (Variety: Pusa Rubi) crop was sown in 4 m x 5 m plots maintaining 30 cm x 30 cm plant distance with three replications and in Randomized Block Design (RBD) during July to October, 2021. The treatments were applied as foliar spray in the form of SC formulation @ 250 ml/ha on initiation of disease incidence and repeat applied at 15 days interval. The active components were applied at 5 doses in different binary combinations. Solo components were also taken for comparison. The treatment details are as under:

Tl - Trifloxystrobin + Valifenalate (l%+80%)
T2 - Trifloxystrobin + Valifenalate (10%+60%)
T3 - Trifloxystrobin + Valifenalate (20%+40%)
T4 - Trifloxystrobin + Valifenalate (30%+20%)
T5 - Trifloxystrobin + Valifenalate (40%+1%)
T6 - Trifloxystrobin (1%)
T7 - Trifloxystrobin (10%)
T8 - Trifloxystrobin (20%)
T9 - Trifloxystrobin (30%)
T10 - Trifloxystrobin (40%)
Til-Valifenalate (1%)
T12-Valifenalate (20%)
T13-Valifenalate (40%)
T14-Valifenalate (60%)
T15-Valifenalate (80%)
T16 - Untreated check
The treatments Tl to T15 were applied as foliar spray and in T16 control treatment only water was sprayed. The observations for early blight disease caused by Alternaria solani and late blight caused by Phytophthora infestans incidence were recorded before first spray and at 10 days after each spray by observing three leaves of randomly selected ten random plants per plot and using 0-9 scale as under.
0-9 Scale for early blight disease
Score Symptoms
0 No symptom on the leaf
1 Small irregular brown spots covering 1% or less of the leaf area
Small, irregular, brown spots with concentric rings covering 1 - 10% of the leaf 3
area
Lesions enlarging, irregular, brown with concentric rings, cover 11 - 25% of leaf
area
Lesions coalesce to form irregular, dark brown patches with concentric rings 7
covering 26 - 50% of leaf area. Lesions on stems and petioles

Lesions coalesce to form irregular, dark brown patches with concentric rings 9
covering more than 51% of leaf area. Lesions on stems and petioles
0-9 Scale for late blight disease Score Symptoms
0 No symptoms observed
Only a few plants affected here and there, upto 1 or 2 spots in 12 yards radius. Upto 10 spots per plant, or general light spotting
About 50 spots per plant, or upto 1 leaflet in 10 attacked
Nearly every leaflet with lesions, plants still retaining normal form; field may smell of blight but looks green, although every plant is affected
Every plant affected and about V2 of leaf area destroyed by blight; field looks green flecked with brown
About 3/4 of leaf area destroyed by blight; field looks either predominantly brown or green. In some varieties the youngest leaves escape infection. Only few leaves left green but stem remain green. All leaves dead, stems dead or drying.
Based on the data recorded per cent disease index (PDI) was calculated. The per cent reduction in disease incidence was also calculated over control. Based on the per cent reduction in disease incidence the Expected control was calculated and compared with Observed control. The results of the trial have been presented here under in Table 16.
Table 16: Synergistic effect of composition of the present invention (Trifloxystrobin + Valifenalate) against early blight and late blight diseases in tomato crop

s.
No. Treatment Formulation Observed per cent control Expected per cent control Colby Ratio

Early blight Late blight Early blight Late blight Early blight Late blight
Tl Trifloxystrobin + Valifenalate 1% + 80% 59.62 57.32 46.21 37.15 1.290 1.543

T2 Trifloxystrobin + Valifenalate 10%+ 60% 61.54 63.41 45.29 37.34 1.359 1.698
T3 Trifloxystrobin + Valifenalate 20% + 40% 63.46 65.85 45.00 37.59 1.410 1.752
T4 Trifloxystrobin + Valifenalate 30% + 20% 65.38 69.51 46.26 38.41 1.413 1.810
T5 Trifloxystrobin + Valifenalate 40% + 1% 56.73 54.88 47.19 38.21 1.202 1.436
T6 Trifloxystrobin 1% 9.62 13.41 - - - -
T7 Trifloxystrobin 10% 18.27 19.51 - - - -
T8 Trifloxystrobin 20% 23.08 23.17 - - - -
T9 Trifloxystrobin 30% 28.85 28.05 - - - -
T10 Trifloxystrobin 40% 35.58 32.93 - - - -
Til Valifenalate 1% 7.69 10.98 - - - -
T12 Valifenalate 20% 17.31 14.63 - - - -
T13 Valifenalate 40% 27.88 23.17 - - - -
T14 Valifenalate 60% 31.73 28.05 - - - -
T15 Valifenalate 80% 37.50 35.37 - - - -
T16 Untreated control - - - - - - -
It is clearly evident from the data shown in above Table 16, for per cent early blight disease control and per cent late blight disease control in tomato crop that the binary composition of the present invention comprising Trifloxystrobin and Valifenalate Tl to T5 are highly synergistic for both the diseases control with > 1 Colby's Ratio. The binary composition of two fungicides are thus highly effective to control early blight and late blight diseases as compared to solo fungicides Trifloxystrobin and Valifenalate (Tl to T5).
Experiment for bio-efficacy evaluation of composition of present invention:
For the binary composition based on Trifloxystrobin and Valifenalate evaluation under field conditions, the basic test composition as Trifloxystrobin 20% SC and Valifenalate 40% SC were manufactured specifically by the inventor. The solo formulations of Trifloxystrobin 20% SC and Valifenalate 40% SC were used for comparison. The binary composition formulation

Tebuconazole 50% + Trifloxystrobin 25% WG available in the market was also used for comparison.
Example 9-
Evaluation of the composition of the present invention on tomato:
The synergistic composition of the present invention Trifloxystrobin 20% + Valifenalate 40% SC @ 300, 350 and 400 ml/ha along with different solo formulations were evaluated on tomato crop variety Pusa Rubi. The experiment was conducted at a farmer field, Sonipat during July to October, 2021 following Randomized Block Design (RBD) with three replications and maintaining a distance of 30 cm x 30 cm between plants and rows. The application of treatments was started with the initiation of early blight and late blight diseases incidence on the crop in experimental plots and repeat application was done after 15 days.
The observations were recorded for the following objectives.
Objectives:
1. Bio-efficacy evaluation against early blight disease on tomato crop
2. Bio-efficacy evaluation against late blight disease on tomato crop
3. Bio-efficacy evaluation based on per cent reduction in early blight disease incidence
4. Bio-efficacy evaluation based on per cent reduction in late blight disease incidence
5. Bio-efficacy evaluation based on tomato fruit yield
6. Phytotoxicity evaluation on tomato crop
7. Economics of treatments based on Cost: Benefit Ratio
Treatment details:
Tl - Trifloxystrobin 20% + Valifenalate 40% SC @ 300 ml/ha T2 - Trifloxystrobin 20% + Valifenalate 40% SC @ 350 ml/ha T3 - Trifloxystrobin 20% + Valifenalate 40% SC @ 400 ml/ha T4 - Trifloxystrobin 20% SC @ 300 ml/ha T5 - Trifloxystrobin 20% SC @ 350 ml/ha T6 - Trifloxystrobin 20% SC @ 400 ml/ha T7 - Valifenalate 40% SC @ 300 ml/ha T8 - Valifenalate 40% SC @ 350 ml/ha T9 - Valifenalate 40% SC @ 400 ml/ha

T10 - Tebuconazole 50% + Trifloxystrobin 25% WG @ 350 ml/ha Til- Untreated control
T12 - Trifloxystrobin 20% + Valifenalate 40% SC @ 800 ml/ha (For phytotoxicity evaluation)
Methodology:
The observations for early blight disease caused by Alternaria solani and late blight disease caused by Phytophthora infestans were recorded before first spray and at 10 days after each spray based on three leaves of randomly selected ten plants per plot following 0-9 scale as under.
0-9 Scale for early blight disease
Score Symptoms
0 No symptom on the leaf
1 Small irregular brown spots covering 1% or less of the leaf area
Small, irregular, brown spots with concentric rings covering 1 - 10% of the leaf 3
area
Lesions enlarging, irregular, brown with concentric rings, cover 11 - 25% of leaf
area
Lesions coalesce to form irregular, dark brown patches with concentric rings 7
covering 26 - 50% of leaf area. Lesions on stems and petioles
Lesions coalesce to form irregular, dark brown patches with concentric rings 9
covering more than 51% of leaf area. Lesions on stems and petioles
0-9 Scale for late blight disease Score Symptoms
0 No symptoms observed
Only a few plants affected here and there, upto 1 or 2 spots in 12 yards radius. Upto 10 spots per plant, or general light spotting
3 About 50 spots per plant, or upto 1 leaflet in 10 attacked

Nearly every leaflet with lesions, plants still retaining normal form; field may smell of blight but looks green, although every plant is affected
Every plant affected and about V2 of leaf area destroyed by blight; field looks green 7
flecked with brown
About 3/4 of leaf area destroyed by blight; field looks either predominantly brown or 9 green. In some varieties the youngest leaves escape infection. Only few leaves left green but stem remain green. All leaves dead, stems dead or drying.
Based on the data recorded per cent disease index (PDI) was calculated. The per cent reduction in disease incidence was also calculated over control. The crop yield was recorded at each harvest and cumulative yield has been expressed as q/ha. The observations for phytotoxicity symptoms on tomato crop at 1, 3, 7 and 10 days after each spray were also recorded. Based on treatment application cost, market price of produce and net profit, the Cost: Benefit Ratio was calculated for the economics of binary composition treatments. The data were subjected to statistically analysis of variance. Results are presented in Tables 17, 18 and 19.
Results:
The results for early blight and late blight disease incidence on tomato crop recorded during the experimental period and per cent reduction in disease incidence over untreated control are summarized in Table 17 and 18. The data on yield is presented in Table 19. The observations recorded for phytotoxicity symptom is presented in Table 20.
Table 17: Field bio-efficacy evaluation of Trifloxystrobin 20% + Valifenalate 40% SC against early blight disease of tomato crop

PDI early blight Per cent disease
s.
No. Treatment A.I.
dose (g/ha) Formulation
dose (ml or
g/ha) reduction

Before spray 10
days
after I 10 days after
II 10 days after
I 10 days after
II
spray spray spray spray

Tl Trifioxystrobin 20% + Valifenalate 40% SC 60 + 120 300 3.83 (11.24) 5.56 (13.60) 6.54 (14.79) 66.67 78.01
T2 Trifioxystrobin 20% + Valifenalate 40% SC 70 + 140 350 4.69 (12.44) 4.94 (12.81) 5.31 (13.26) 70.37 82.16
T3 Trifioxystrobin 20% + Valifenalate 40% SC 80 + 160 400 4.32 (11.98) 4.57 (12.33) 5.06 (12.99) 72.59 82.99
T4 Trifioxystrobin 20% SC 60 300 4.57 (12.30) 7.53 (15.91) 9.88 (18.31) 54.81 66.80
T5 Trifioxystrobin 20% SC 70 350 4.07 (11.59) 6.91 (15.23) 9.75 (18.18) 58.52 67.22
T6 Trifioxystrobin 20% SC 80 400 3.95 (11.41) 6.54 (14.79) 9.63 (18.06) 60.74 67.63
T7 Valifenalate 40% SC 120 300 4.32 (11.95) 7.41 (15.75) 9.51 (17.94) 55.56 68.05
T8 Valifenalate 40% SC 140 350 4.44 (12.16) 6.67 (14.94) 9.38 (17.82) 60.00 68.46
T9 Valifenalate 40% SC 160 400 4.81 (12.63) 6.42 (14.67) 9.14
(17.57) 61.48 69.29
T10 Tebuconazole 50% +
Trifioxystrobin 25% WG 175 + 87.5 350 4.20 (11.75) 5.80 (13.88) 6.79 (15.08) 65.19 77.18
Til Untreated control - - 4.32 (11.97) 16.67 (24.08) 29.75 (33.05) - -
SEm± 0.75 0.69 0.64
CD (P=0.05) NS 2.02 1.88
Figures in parentheses are angular transformed values NS - Non significant

Table 18: Field bio-efficacy evaluation of Trifloxystrobin 20% + Valifenalate 40% SC against late blight disease of tomato crop

s.
No. Treatment A.I.
dose (g/ha) Formulation
dose (ml or
g/ha) PDI early blight Per cent
disease
reduction

Before spray 10
days
after I
spray 10 days after
II spray 10 days after
I spray 10 days after
II spray
Tl Trifloxystrobin 20% + Valifenalate 40% SC 60 + 120 300 3.46 (10.61) 4.69 (12.48) 5.93 (14.06) 70.31 79.31
T2 Trifloxystrobin 20% + Valifenalate 40% SC 70 + 140 350 3.58 (10.82) 3.95 (11.44) 4.44 (12.16) 75.00 84.48
T3 Trifloxystrobin 20% + Valifenalate 40% SC 80 + 160 400 3.21 (10.27) 3.58 (10.87) 4.20 (11.79) 77.34 85.34
T4 Trifloxystrobin 20% SC 60 300 3.70 (11.06) 6.67 (14.95) 9.14 (17.59) 57.81 68.10
T5 Trifloxystrobin 20% SC 70 350 2.59 (9.25) 6.30 (14.52) 8.89
(17.32) 60.16 68.97
T6 Trifloxystrobin 20% SC 80 400 2.96 (9.87) 6.17 (14.38) 8.77 (17.21) 60.94 69.40
T7 Valifenalate 40% SC 120 300 3.46 (10.67) 6.42 (14.64) 9.01 (17.46) 59.38 68.53
T8 Valifenalate 40% SC 140 350 3.33 (10.51) 6.30 (14.52) 8.77 (17.21) 60.16 69.40

T9 Valifenalate 40% SC 160 400 3.70 (11.09) 5.93 (14.08) 8.52 (16.95) 62.50 70.26
T10 Tebuconazole 50% +
Trifloxystrobin 25% WG 175 + 87.5 350 3.09 (10.09) 4.94 (12.80) 6.42 (14.67) 68.75 77.59
Til Untreated control - - 2.84 (9.67) 15.80
(23.42) 28.64
(32.35) - -
SEm± 0.66 0.56 0.56
CD (P=0.05) NS 1.64 1.65
Figures in parentheses are angular transformed values NS - Non significant
Table 19: Field bio-efficacy evaluation of Trifloxystrobin 20% + Valifenalate 40% SC based on tomato fruit yield and Cost: Benefit Ratio

s.
No. Treatment A.I. dose (g/ha) Formulatio
n dose (ml
or g/ha) Yield (q/ha) Per cent increase in yield over
control C:B
Ratio
Tl Trifloxystrobin 20% + Valifenalate 40% SC 60 + 120 300 169.67 (13.02) 38.69 1 : 20.45
T2 Trifloxystrobin 20% + Valifenalate 40% SC 70 + 140 350 174.33 (13.20) 42.51 1 : 21.64
T3 Trifloxystrobin 20% + Valifenalate 40% SC 80+ 160 400 176.33 (13.28) 44.14 1 : 21.63
T4 Trifloxystrobin 20% SC 60 300 151.50 (12.30) 23.84 1 : 12.46
T5 Trifloxystrobin 20% SC 70 350 153.17 (12.37) 25.20 1 : 12.70
T6 Trifloxystrobin 20% SC 80 400 157.17 (12.54) 28.47 1 : 13.93
T7 Valifenalate 40% SC 120 300 156.83 (12.51) 28.20 1 : 13.58

T8 Valifenalate 40% SC 140 350 160.33 (12.66) 31.06 1 : 14.30
T9 Valifenalate 40% SC 160 400 162.17 (12.73) 32.56 1 : 14.32
T10 Tebuconazole 50% + Trifloxystrobin 25% WG 175 + 87.5 350 160.00 (12.65) 30.79 1 : 17.68
Til Untreated control - - 122.33 (11.05) - -
SEm± 0.26
CD (P=0.05) 0.78
Figures in parentheses are square root transformed values
"able 20: Phytotoxicity evaluation of Trifloxystrobin 20% + Valifenalate 40% SC on tomato cro]

s.
No. Treatment A.I.
dose (g/ha) Formu-lation
dose (ml or
g/ha) Phytotoxicity parameters observed*
(mean data recorded at 1, 3, 7 and 10 days after each spray)

L w N V E H
Tl Trifloxystrobin 20% + Valifenalate 40% SC 60 + 120 300 0 0 0 0 0 0
T2 Trifloxystrobin 20% + Valifenalate 40% SC 70 + 140 350 0 0 0 0 0 0
T3 Trifloxystrobin 20% + Valifenalate 40% SC 80 + 160 400 0 0 0 0 0 0
T4 Trifloxystrobin 20% SC 60 300 0 0 0 0 0 0
T5 Trifloxystrobin 20% SC 70 350 0 0 0 0 0 0
T6 Trifloxystrobin 20% SC 80 400 0 0 0 0 0 0
T7 Valifenalate 40% SC 120 300 0 0 0 0 0 0

T8 Valifenalate 40% SC 140 350 0 0 0 0 0 0
T9 Valifenalate 40% SC 160 400 0 0 0 0 0 0
T10 Tebuconazole 50% + Trifloxystrobin 25% WG 175 + 87.5 350 0 0 0 0 0 0
Til Untreated control - - 0 0 0 0 0 0
T12 Trifloxystrobin 20% + Valifenalate 40% SC 160 + 320 800 0 0 0 0 0 0
L - Leaf injury on tips/ surface, W - Wilting, N - Necrosis, V - Vein clearing, E - Epinasty, H - Hyponasty
*Based on 0-10 scale where: 0=0%, 1=1-10%, 2=11-20%, 3=21-30%, 4=31-40%, 5=41-50%, 6=51-60%, 7=61-70%, 8=71-80%, 9=81-90%, 10=91-100%
From the above examples, it can be clearly seen that the composition of the present invention is superior for controlling the early blight and late blight diseases, at 10 days after each spray (Table 17 and 18) in tomato crop as compared to the solo component comprising Trifloxystrobin 20% SC and Valifenalate 40% SC at different dosage levels. Other registered formulated product available in the market, Tebuconazole 50% + Trifloxystrobin 25% WG evaluated was next in order of effectiveness after binary composition. The yield of tomato fruit also improved in the binary composition of present invention as compared to solo components (Table 19). One of the reasons of higher crop yield may be due to better control of diseases. The Cost: Benefit Ratio revealed that binary composition Trifloxystrobin 20% + Valifenalate 40%) SC @ 350 ml/ha and 400 ml/ha were most economical with higher Cost: Benefit Ratio as compared to other binary and solo treatments (Table 19).
The disclosed composition of Trifloxystrobin 20% + Valifenalate 40% SC @ 300, 350, 400 and 800 ml/ha further showed no phytotoxicity in the tomato crop after 1,3,7 and 10 days after each spray (Table 20).
It is evident from the above tables of examples that the composition of the present invention resulted in efficient control of early blight and late blight diseases with higher yield as compared to the reference standard products (market available binary and solo products). The Cost: Benefit Ratio also showed that binary composition Trifloxystrobin 20% + Valifenalate 40%) SC @ 350 and 400 ml/ha was most economical with higher Cost: Benefit Ratio as

compared to other binary and solo treatments. Further, the composition of present invention is resulted synergistic.
Thus, from the above, it will be observed that numerous modifications and variations can be effectuated without departing from the true spirit and scope of the novel concepts of the present invention. It is to be understood that no limitations with respect to the specific embodiments illustrated is intended or should be inferred. It should be understood that all such modifications and improvements have been deleted herein for the sake of conciseness and readability but are properly within the scope of the following claims.
It is to be understood that the present invention is susceptible to modifications, changes and adaptations by those skilled in the art. Such modifications, changes, adaptations are intended to be within the scope of the present invention.
Advantages of the present invention:
1. A significantly lower incidence of early blight and late blight disease in tomato crops.
2. Improved yield, economical i.e., higher Cost: Benefit ratio.
3. No phytotoxic effect.
4. Highly effective in improving yield, and controlling diseases at lower doses.

WE CLAIM:

1. A synergistic fungicidal composition comprising, as active ingredients, (a) Valifenalate or its agrochemically acceptable salts and (b) at least one fungicide selected from Strobilurin fungicides or their agrochemically acceptable salts.
2. The fungicidal composition as claimed in claim 1, wherein the at least one Strobilurin fungicide is selected from Azoxystrobin, Pyraclostrobin, Fluoxastrobin, Kresoxim-methyl, Trifloxystrobin, Picoxystrobin, Mandestrobin, and Metominostrobin or their agrochemically acceptable salts.
3. The fungicidal composition as claimed in claim 1, wherein the composition comprises of:
a. Valifenalate or its agrochemically acceptable salts in an amount in the range
from 1 to 80% by weight of the composition;
b. at least one strobilurin fungicide or its agrochemically acceptable salts in an
amount in the range from 1 to 40% by weight of the composition.
4. The fungicidal composition as claimed in claim 2, wherein the composition comprises
of:
a. Valifenalate or its agrochemically acceptable salts in an amount in the range
from 1 to 80%) by weight of the composition;
b. Trifloxystrobin or its agrochemically acceptable salts as strobilurin fungicide in
an amount in the range from 1 to 40%> by weight of the composition.
5. The fungicidal composition as claimed in any one of the claims 1 to 4, which further comprises one or more agrochemically acceptable excipients.
6. The fungicidal composition as claimed in any one of the claims 1 to 5, wherein the agrochemically acceptable excipients are selected from group comprising surfactants/dispersing agents, polymers, anti-freezing agent, wetting agents, anti-foaming agents, colorants, pigments, binder/structuring agents, biocides/anti-microbial agent, thickeners, emulsifiers, buffering agent, fillers, diluents/solvents, quick coating

agents or sticking agents, preservatives, adjuvants and solvents or a combination thereof.
7. The fungicidal composition as claimed in claim 6, wherein the thickeners are selected from the group comprising silicates such metal silicates, aluminum magnesium silicate sodium carboxy methyl cellulose, methyl cellulose, ethyl cellulose, poly vinyl alcohol, sodium alginate, sodium poly acrylate, xanthan gum, welan gum, gum arabic, montmorillonite, lingo sulfonates, hydroxy methyl cellulose, dextrin, starch, Bentonite Clay, Polysaccharides or combinations thereof.
8. The fungicidal composition as claimed in claim 6, wherein the anti-freezing agent is selected from the group comprising polyethylene glycols, methoxy polyethylene glycols, propylene glycol, polypropylene glycols, polybutylene glycols, Mono ethylene glycol, glycerin, ethylene glycol, Glycerin, Diethylene glycol or combinations thereof.
9. The fungicidal composition as claimed in claim 6, wherein the anti-foaming agent is selected from the group comprising silicone oils, or mineral oils, Fatty acid ester; potassium sorbate, and parahydroxy benzoates, silicon emulsion based anti-foam agents, Siloxane polyalkylene oxide, Polydimethyl Siloxane, tri siloxane ethoxylates or combinations thereof.
10. The fungicidal composition as claimed in claim 6, wherein the wetting agent is selected from the group comprising Tristyrylphenol ethoxylate nonionic emulsifier, Mixture of non-ionic surfactants & Alkoxylated Alcohol/Block copolymer, sodium lauryl sulphate; sodium dioctyl sulphosuccinate; Tristyrylphenol ethoxylate non-ionic emulsifier/ mixture of non-ionic surfactants and alkoxylated Alcohol/Block copolymer, ionic surfactant mixtures, anionic surfactants, alkyl phenol ethoxylates, aliphatic alcohol ethoxylates and the salts or combinations thereof.
11. The fungicidal composition as claimed in claim 6, wherein the polymers are selected from the group comprising polyvinyl alcohols, polyvinylpyrrolidone, gel-forming carraagheenans, water-soluble gelatine and casein, superabsorbent polymers such polyacrylamides and polyacrylates based polymers, semi-synthetic or fully-synthetic peptide/protein-based superabsorbent polymers such as collagen-based synthetic

polymers, elastin-like polypeptides, polyaspartic acid, polyaspartates, polyglutamic acid, polyglutamate, Semi-synthetic or fully-synthetic polysaccharide: carboxymethyl starch , sulfoethyl starch, carboxymethyl cellulose, sulfoethyl cellulos, hydroxypropyl cellulose, hydroxyethyl cellulose, methylcellulose, chitosan, acrylonitrile, acrylic acid, methacrylic acid, acrylamide, methacrylamide, 2-acrylamido-2-methyl-propanesulfonic acid (AMPS), vinyl sulfonic acid, ethyl acrylate, and potassium acrylate or combinations thereof.
12. The fungicidal composition as claimed in claim 6, wherein the adjuvant is selected from the group comprising Silicone Ethoxylated Oil, Polyvinyl Pyrrolidon,Poly vinyl Alcohol,Blend of poly terpene resin or combination thereof.
13. The fungicidal composition as claimed in claim 6, wherein the diluent/solvents are selected from the group comprising lactose, glucose, fructose, maltose, sucrose, in the anhydrous or hydrate forms, urea, water soluble or dispersible polymers, water soluble inorganic salts, Demineralized (DM) water, Vegetable Oil or combination thereof.
14. The fungicidal composition as claimed in claim 6, wherein the filler is selected from the group comprising Silicon Dioxide, China -Clay, Kaolin, Talc, starch or combination thereof.
15. The fungicidal composition as claimed in claim 6, wherein the biocide/anti-microbial agent is selected from the group comprising Benzisothiazolin-3-one / Formaldehyde/Sodium benzoate/Sodium o-phenylphenate, 5-chloro-2-methyl-4-isothiazolin-3-one & 2-methyl-4-isothiazolin-3-one or combination thereof.
16. The fungicidal composition as claimed in any one of the claims 1 to 6, wherein the composition is in form selected from Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable Slurry (FS), Flowable Suspension(FS), Suspension Concentrate (SC), Suspension concentrate for direct application (SD), Suspo- emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Water soluble powder (SP), Water dispersible powder

for slurry seed treatment (WS), Water dispersible granules (WDG), Wettable powders (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), granular (GR), oil in dispersion (OD), a mixed formulation of CS and SC (ZC) or a mixed formulation of CS and SE (ZE), a mixed formulation of CS and EW (ZW).
17. A method for controlling a plant disease caused by a fungal plant pathogens comprising applying to the plant or portion thereof or soil, a fungicidally effective amount of the fungicidal composition as claimed in any one of the claims 1-16.
18. A method of improving vigour or yield of the plant comprising, treating a plant or portion thereof or soil, a fungicidally effective amount of the fungicidal composition as claimed in any one of the claims 1-16.
19. The method as claimed in claim 17 or 18, wherein the plant is selected from the group consisting of cereals, field crops, seeds, fruits, tree nuts, vegetables, turf grasses, potatoes, tomatoes, grapes and ornamentals.
20. A process for preparing a stable synergistic fungicidal composition as claimed in claims 1-16.