DESC:FIELD OF INVENTION

The present invention relates to synergistic mixtures of Mepiquat, Triazole and Strobilurin fungicides, wherein Triazoles may be selected from the group consisting of Difenoconazole, Epoxiconazole, Flusilazole, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole or Tricyclazole and Strobilurin fungicides may be selected from the group consisting of Azoxystrobin, Trifloxystrobin, Metominostrobin, Pyraclostrobin, Picoxystrobin, Orysastrobin, Coumoxystrobin or Kresoxim Methyl. The present invention more particularly relates to fungicidal compositions and a method for treating plants using the said composition.

BACKGROUND OF THE INVENTION

Fungicidal compositions comprising Strobilurin fungicides and Triazole fungicides are known in the literature.

WO2015/139564 relates to a fungicidal composition comprising components (A) at least one strobilurin fungicides and (B) at least one triazole fungicides.

CN103798246 relates to pesticide suspension concentrate comprising difenoconazole and azoxystrobin.

WO2007/009775 describes a mixture comprising (A) Tebuconazole; and (B) one or more compounds selected from Carboxin, Chlorothalonil, Difenoconazole, Azoxystrobin, Fluquinconazole, Metalaxyl, Mefenoxam, Thiram, Abamectin, Lambda-cyhalothrin, Beta-cyflutrin, Tefluthrin, Thiamethoxam, Flubendamide and a compound of formula (A-1) and (C) one or more customary formulation adjuvants.

WO2007/028537 describes a mixture comprising provides highly concentrated WG formulations of fungicidal mixtures, comprising Trifloxystrobin and an active ingredient selected from tebuconazole and Tolylfluanid.
WO2003/045150 relates to an agrochemical composition for the treatment of plant propagation material, comprising at least two active ingredient components together with a suitable carrier, wherein component I is I) difenoconazole and component II is IIA) azoxystrobin or IIB) picoxystrobin or IIC) kresoxim-methyl.

Plant growth regulators (PGRs) are generally substances or mixtures of substances intended to accelerate or retard the rate of growth or maturation, or otherwise alter the development of plants or their produce. Plant growth regulators (PGRs) affect growth and differentiation of plants. More specifically, various PGRs can, for example, reduce plant height, stimulate seed germination, induce flowering, darken leaf coloring, change the rate of plant growth and modify the timing and efficiency of fruiting. Plant growth regulators (PGRs) regulate growth, check the vegetative growth and enhance the production by increasing flowers, fruits etc and quality.

PGRs are recognized as an essential tool in modern cotton production. Mepiquat (IUPAC name: N,N-dimethylpiperidinium) is a widely accepted PGR for cotton plants and is typically applied to cotton plants in the form of a salt, such as mepiquat chloride or mepiquat pentaborate, by foliar application. U.S. Patent Nos. 5,478,796; 5,627,134; 5,650,372; 5,654,255; 5,869,424; 5,935,906; 6,224,734; 6,232,270; 6,248,694; 6,288,009; 6,376,425 and 6,465,394 teach various mepiquat formulations.

WO2007/001919 relates to mixtures of strobilurin fungicides and plant growth regulators and to the application of these mixtures to crops including the foliar application of these mixtures on cotton.

4206/KOLNP/2009 (WO2008/152096) relates to the use of at least one fungicide selected from terocyclylamiden among aryl and heteroaryl, carbamates, dicarboximides, azoles, strobilurins and morpholines, where appropriate. In combination with at least one growth regulator, to achieve a more uniform temporal sequence of phenological development of oil plants.
US2012/0137941 discloses a mixture comprising at least two active compounds (A) selected from the group consisting of mepiquat chloride, chlormequat chloride, N, N-dimethylmorpholinium chloride, metconazole, tebuconazole, paclobutrazol, trinexapac and prohexadion or an agriculturally useful salt thereof to the seeded crop variety and t least one compound (B) selected from boscalid, dimoxystrobin, difenoconazole, prothioconazole, prochloraz, thiophanat-methyl and iprodione.

ADVANTAGES OF THE PRESENT INVENTION:

• Increase in yield of treated plants (cereals, pulses, oilseeds, fibre crop, sugar crops, leafy vegetables, tuber crops, fruit crops, flowers, ornamentals etc).
• Increase in yield due to protection against fungal diseases.
• Increase in yield due to plant growth regulation, check vegetative growth and increase in reproductive parts of the plant.
• Increase in yield due to more number of tillers, more branches and sub branches, more number of flowers, and more number of fruits.
• Increase plant vigor.
• Increase tolerance to insect-pests damage.
• Increase tolerance to the weather stress, moisture stress.
• Prevents lodging in susceptible plants (lodging due to biotic and abiotic factors, like heavy rains, winds, insects and diseases damage).
• Improves quality (means visual appearance, color, size, shape etc.) in grains, fruits, fiber, flowers, tuber, bulb, rhizomes, straw, leaves and other plant parts and plant products.
• Improves keeping the quality of produce, increase post harvest life, storage life, protection from post harvest diseases.
• Uniform sizing in tuber, bulb, rhizome and root crops.

OBJECTS OF THE INVENTION

It is an object of the present invention to provide fungicidal compositions comprising Mepiquat, Triazole fungicides and Strobilurin fungicides.

Another object of the present invention is to provide fungicidal compositions comprising Mepiquat, Triazole fungicides and Strobilurin fungicides which improved the yield of the treated plants (cereals, pulses, oilseeds, fibre crop, sugar crops, leafy vegetables, tuber crops, fruit crops, flowers, ornamentals etc.)

SUMMARY OF THE INVENTION

According to an aspect of the present invention there is provided a synergistic fungicidal composition comprising:

I) Mepiquat or a salt thereof in the range from 1 to 20 % by weight of the composition;
II) triazole compound in the range from 1 to 40% by weight of the composition wherein the said triazole compound is selected from the group consisiting of Difenoconazole, Epoxiconazole, Flusilazole, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole or Tricyclazole;
III) strobilurin compound in the range from 1 to 40 % by weight of the composition wherein the said strobilurin compound is selected from the group consisting of Azoxystrobin, Trifloxystrobin, Metominostrobin, Pyraclostrobin, Picoxystrobin, Orysastrobin, Coumoxystrobin and Kresoxim Methyl;
IV) One or more inactive excipient.

DETAILED DESCRIPTION OF THE INVENTION

The present invention relates to mixtures of Mepiquat, triazole fungicides and strobilurin fungicides.

In an embodiment of the present invention there is provided a synergistic fungicidal composition comprising:

I) Mepiquat;
II) at least one triazole compound;
III) at least one strobilurin compound.

In an embodiment of the present invention there is provided a synergistic fungicidal composition comprising Mepiquat, Triazole compound and Strobilurin compound with the following mass percentage of the composition:

Sr. No. Raw material Concentration range (w/w %)
i Mepiquat 1 to 20
ii Triazole compound 1 to 40
iii Strobilurin compound 1 to 40

The Triazole is selected from the group consisting of Difenoconazole, Epoxiconazole, Flusilazole, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole and Tricyclazole.

The strobilurin is selected from the group consisting of Azoxystrobin, Coumoxystrobin, Kresoxim methyl, Metominostrobin, Orysastrobin, Picoxystrobin, Pyraclostrobin and Trifloxystrobin.

In an embodiment of the present invention there is provided a synergistic fungicidal composition comprising:

I) Mepiquat or salt thereof in the range from 1 to 20 % by weight of the composition;
II) triazole compound in the range from 1 to 40% by weight of the composition wherein the said triazole compound is selected from the group consisiting of Difenoconazole, Epoxiconazole, Flusilazole, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole or Tricyclazole;
III) strobilurin compound in the range from 1 to 40 % by weight of the composition wherein the said strobilurin compound is selected from the group consisting of Azoxystrobin, Trifloxystrobin, Metominostrobin, Pyraclostrobin, Picoxystrobin, Orysastrobin, Coumoxystrobin and Kresoxim Methyl;
IV) One or more inactive excipient.

The inactive excipients is selected from the group consisting of but not limited to defoamer/anti-foaming agent, biocide/preservative, emulsifier, spreader, wetting agent, dispersant, co-dispersant, suspending agents, anti-freezing agent, anti caking agent, rheology modifier/thickener, binder, carrier or filler.

List of inactive agent which may be used in the present invention are listed below but not limited to:

Inactive agent Function
Acrylic graft copolymer Dispersant
Ethoxylated Fatty Alcohol co-dispersant/wetting agent
polyalkoxy alkyl ether Dispersing agent ,Emulsifier
ethylene oxide/propylene oxide block copolymer dispersing agent,emulsifier
sodium salt of naphthalene sulfonate condensate Despersant
Trisiloxane ethoxylate Spreader & wetting agent
Sodium ligno sulfonate Dispersant
silicone antifoam emulsion Antifoaming/defoamer
Silicone dioxide Suspending aid,anti caking agent
polysaccharide Rheology Modifier/Thickner
Sodium dioctyl sulfosuccinate wetting agent and emulsifier
Polyarylphenyl ether phosphate Dispersing, wetting agent
DiEthylene glycol Antifreezing Agent
MonoEthylene Glycol Antifreezing Agent
Polypropanil Glycol Antifreezing Agent
Glycerene Antifreezing Agent
PolyEthylene Glycol Antifreezing Agent
1,2-benzisothiazolin-3-one Biocide/preservative
Sodium benzoate Biocide/preservative
2-Bromo-2-nitro-1,3-propanediol Biocide/preservative
Formaldehyde Biocide/preservative
Aluminum Magnesium Silicate Suspending aid
sodium salt of naphthalene sulfonate condensate Dispersing Agent
blend of naphthalene sulfonate condensate Wetting Agent
Sodium dodecyl benzene sulfonate wetting agent
Blend of naphthalene sulfonate condensate & phenol sulfonate condensate Wetting Agent & dispersing agent
sodium lauryl sulfate Wetting Agent
Corn starch Binder, filler
Lactose Filler/carrier
Polyvinylpyrrolidone Binder
Modified polyacrylate copolymer Dispersing Agent
Sodium polycarboxylate Dispersing Agent
Sodium Ligno sulfonate Dispersing Agent
China clay/kaoline Filler/carrier
silicone antifoam emulsion Anto-foaming/Defoamer
Silicon dioxide Suspending agent, Anticacking Agent,Filler
Titanium dioxide Filler/carrier
talcom powder Filler/carrier
Urea formaldehyde resin Anti caking agent
Diatomaceous earth Filler/carrier

The fungicidal composition of the present invention can be formulated in the forms given below:

a) Dry - Sprayable

a.1) WP – Wettable powders:

A solid pesticide formulation – micronized to powder form and typically applied as suspended particles after dispersion in water.

a.2) WG or WDG – Water dispersible granule:

A pesticidal formulation consisting of granules to be applied after disintegration and dispersion in water. Water dispersible granules can be formed by a) agglomeration, b) spray drying, or c) extrusion techniques. It can also be termed as water soluble granules (WSG) or soluble granules (SG).

b) Liquid Sprayable

b.1) SL – Soluble Concentrate:

A soluble concentrate is in powder form intended for dilution with water or directly in solution from. In both the case, the end result will be a clear solution of the insecticide in the water without any visible un-dissolved particles.

b.2) SC – Suspension Concentrate:

A stable suspension of solid pesticide(s) in a fluid usually intended for dilution with water before use. For a good formulation or ideal SC formulation, it should be stable and do not sediment over time.

b.3) EC – Emulsifiable Concentrate:

A solution of a pesticide with emulsifying agents in a water insoluble organic solvent which will form an emulsion when added to water. In most of the case it is oil in water type emulsion to make it easy for application. EC formulation should be storage stable without any visible cracking of emulsion.

b.4) ME – Microemulsion:

A solution of a pesticide with emulsifying agents in a water insoluble organic solvent which will form a solution/emulsion when added to water. The difference between EC and ME is the particle size of the actives in the final form.

b.5) OD – Oil Dispersion:

Oil dispersions (OD) are one type of liquid formulation which is stable suspensions of active ingredients in a water-immiscible fluid which may contain other dissolved active ingredients and is intended for dilution with water before use.

b.6) CS – Capsule Suspension:

Suspension of micro-encapsulated active ingredient in an aqueous continuous phase, intended for dilution with water before use.

b.7) SE- Suspension emulsion:

A suspension emulsion or suspo emulsion (SE) consists of an organic phase with a dissolved active ingredient and an aqueous suspension phase, in which the active ingredient is dispersed in water.

b.8) Dispersible concentrate (DC):

Dispersible concentrate (DC) is a liquid homogeneous formulation to be applied as a solid dispersion after dilution in water. There are many formulations which contains the part characteristics of EC and part characteristics of DC.

c) Dry – Spreadable Granule:

Dry spreadable granules are dry granules which can be applied with a dry spreader to a target area and later when such granules get exposed to water via, for example, rain or irrigation, will not only readily disintegrate, but actively spread on solid substrates so as to achieve disintegration area diameter to original granule diameter ratios.

Dry spreadable granules should possess good hardness and an ability to maintain integrity upon normal, commercial handling in a dry spreading operation and yet be capable of quickly disintegrating or scattering upon what may be a minimal exposure to water, such as, for example, a light rain.

d) GR – Soil applied Granule on inert or fertilizer carrier

This formulation is in the form of granules which can be applied on inert carrier or the carrier which is fertilizer.

e) Mixed formulation

e.1) ZC Formulation (Mix of CS and SC):

“ZC formulation” is the international denominations adopted by the FAO (Food and Agricultural Organization of the United Nations) to designate “stable aqueous suspension of microcapsules and solid fine particles.”

ZC is a mixed formulation of CS and SC and is a stable aqueous suspension of microcapsules and solid fine particles, each of which contains one or more active ingredients. The formulation is intended for dilution into water prior to spray application. Formulating the active ingredients together eliminates the need for tank mixing, which can lead to incompatibility, and facilitates control of a wider range of pests with fewer applications. Like other aqueous liquid formulations, ZC formulations are easy to handle and measure, dust free, non-flammable and offer good miscibility with water.

The method of application of the fungicidal composition of the present invention includes foliar spray, Seed treatment or treatment to planting materials.

In an embodiment of the present invention there is provided a process for the preparation of Suspension Concentrate (SC) of the formulation of the present invention comprising the steps of.

(i) Take required quantity of water, biocide, and defoamer and homogenize, then slowly add gum powder to it and stir till complete dissolution. Preparing Gum Solution 12 to 18 hours prior to use.
(ii) Charging required quantity of Demineralised water (DM) water which is needed to be taken in designated vessel for Flowable concentrate/ Suspension concentrate/ Flowable slurry production.
(iii) Adding required quantity of Wetting agent, dispersing agent & suspending agents, colourant/dye and homogenize the contents for 45 to 60 minutes using high shear homogenizer.
(iv) Adding technical and other remaining adjuvants excluding ‘antifreeze & thickeners’ and homogenized to get uniform slurry ready for grinding.
(v) Before grinding half the quantity of antifoam was added and then the material was subjected to three cycles of grinding in Dyno mill.
(vi) The half quantity of the antifoam was added along with antifreeze after grinding process completes and before sampling for in process analysis.
(vii) Finally add gum solution to this formulation and send to QC for quality check.

In another embodiment of the present invention there is provided a process for the preparation of Wettable Granules (WG) of the formulation of the present invention comprising the steps of.

(i) Charging the required quantity of filler, wetting agent, dispersing agent, and suspending agent, & technical in a premixing blender for homogenization for 30 minutes.
(ii) The pre-blended material is than grinded through Jet mill/ air classifier mills. The Finely grinded material is blended in post blender till it becomes homogeneous. (for approx 1.5 hr)
(iii) The homogeneous material is analysed. After getting approval from QC department material is unloaded into 25 kg. HDPE bag with LDPE liner inside.
(iv) The Finely grinded powder is mix with required quantity of water to form extrudable dough.
(v) The dough is passed through an extruder to get granules of required size.
(vi) Wet granules are passed through Fluidised bed drier and further graded using vibrating screens.
(vii) The final product is sent for QC approval.
(viii) After approval material is packed in requied pack sizes.

The fungicidal composition of the present invention can be used for the treatment of following diseases in plants.

Diseases of Wheat: Alternaria leaf blight Alternaria triticina, Anthracnose Colletotrichum graminicola,Ascochyta leaf spot Ascochyta tritici, Aureobasidium decay Microdochium bolleyi, Black head molds (sooty molds) Alternaria spp., Cladosporium spp., Epicoccum spp., Sporobolomyces spp., Stemphylium spp., Common bunt/ stinking smut Tilletia tritici,Common root rot Bipolaris sorokiniana, Cottony snow mold Coprinus psychromorbidus, Crown rot (foot rot, seedling blight, dryland root rot) Fusarium spp., Fusarium pseudograminearum, Fusarium graminearum, Fusarium culmorum,Dilophospora leaf spot Dilophospora alopecuri, Downy mildew = crazy top Sclerophthora macrospora, Dwarf bunt Tilletia controversa, Ergot Claviceps purpurea, Eyespot = foot rot, Tapesia yallundae, False eyespot Gibellina cerealis,Flag smut Urocystis agropyri, Foot rot Fusarium spp., Karnal bunt = partial bunt Tilletia indica =Neovossia indica, Leaf rust = brown rust Puccinia triticina,Puccinia recondite,Leptosphaeria leaf spot Phaeosphaeria herpotrichoides = Leptosphaeria herpotrichoides,Loose smut Ustilago tritici,Microscopica leaf spot Phaeosphaeria microscopica, Phoma spot Phoma spp., Pink snow mold = Fusarium patch Microdochium nivale,Platyspora leaf spot Clathrospora pentamera,Powdery mildew Erysiphe graminis, Blumeria graminis,Pythium root rot Pythium aphanidermatum, Pythium arrhenomanes, Pythium graminicola, Pythium myriotylum,Rhizoctonia root rot Rhizoctonia solani, Scab = head blight Fusarium graminearum, Sclerotinia snow mold = snow scald Sclerotinia borealis,Sclerotium wilt Sclerotium rolfsii,Septoria blotch Septoria tritici,Sharp eyespot Rhizoctonia cerealis, Snow rot Pythium spp., Speckled snow mold = gray snow mold or Typhula blight Typhula idahoensis,Spot blotch Bipolaris sorokiniana,Stagonospora blotch Phaeosphaeria avenaria, Stagonospora avenae, Septoria avenae, Septoria nodorum , Stem rust = black rust Puccinia graminis,Storage molds Aspergillus spp., Penicillium spp., Stripe rust = yellow rust Puccinia striiformis,Take-all Gaeumannomyces graminis,Tan spot = yellow leaf spot, red smudge Pyrenophora tritici-repentis, Drechslera tritici-repentis,Tar spot Phyllachora graminis, powdery mildew (Erysiphe graminis), Fusarium head blight (Fusarium graminearum), rust (Puccinia striiformis, Puccinia graminis, Puccinia recondita), loose smut (Ustilago tritici), eye spot disease (Pseudocercosporella herpotrichoides), leaf blight (Mycosphaerella graminicola), yellow leaf blotch (Pyrenophora tritici-repentis).

Diseases of Rice: Aggregate sheath spot Ceratobasidium oryzae (anamorph: Rhizoctonia oryzae-sativae), Black kernel Curvularia lunata, Blast (leaf, neck [rotten neck], nodal and collar) Pyricularia grisea=P.oryzae,Brown spot Cochliobolus miyabeanus (anamorph: Bipolaris oryzae),Crown sheath rot Gaeumannomyces graminis,Downy mildew Sclerophthora macrospora, Eyespot Drechslera gigantean,False smut Ustilaginoidea virens,Kernel smut Tilletia barclayana =Neovossia horrid, Leaf smut Entyloma oryzae, Leaf scald Microdochium oryzae, Narrow brown leaf spot Cercospora janseana, Pecky rice (kernel spotting) Damage by many fungi including Cochliobolus miyabeanus, Curvularia spp., Fusarium spp., Microdochium oryzae, Sarocladium oryzae, Root rots Fusarium spp., Pythium spp., P. dissotocum, P. spinosum,Seedling blight Cochliobolus miyabeanus, Curvularia spp., Fusarium spp., Rhizoctonia solani, Sclerotium rolfsii,Sheath blight Thanatephorus cucumeris (anamorph: Rhizoctonia solani), Sheath rot Sarocladium oryzae = Acrocylindrium oryzae, Sheath spot Rhizoctonia oryzae, Stackburn (Alternaria leaf spot) Alternaria padwickii, Stem rot Magnaporthe salvinii= Sclerotium oryzae, Water-mold (seed-rot and seedling disease) Achlya conspicua, A. klebsiana, Fusarium spp., Pythium spp., P. dissotocum, P. spinosum., Sheath blight (Rhizoctonia solani), Blast (Pyricularia oryzae), Helminthosporium leaf spot, Bakanae disease (Gibberella fujikuroi).

Diseases of Corn or Maize: Anthracnose leaf blight and anthracnose stalk rot Colletotrichum graminicola, Aspergillus ear and kernel rot Aspergillus flavus, Banded leaf and sheath spot Rhizoctonia solani= Rhizoctonia microsclerotia), Black bundle disease Acremonium strictum, Black kernel rot Lasiodiplodia theobromae = Botryodiplodia theobromae, Brown spot (black spot, stalk rot) Physoderma maydis, Cephalosporium kernel rot Acremonium strictum=Cephalosporium acremonium, Charcoal rot Macrophomina phaseolina, Corticium ear rot Thanatephorus cucumeris= Corticium sasaki, Curvularia leaf spot Curvularia clavata,C. eragrostidis, Curvularia inaequalis, C. intermedia, Curvularia lunata, Curvularia pallescens , Curvularia senegalensis, C. tuberculata, Didymella leaf spot Didymella exitalis, Diplodia ear rot and stalk rot Diplodia frumenti,Diplodia ear rot, stalk rot, seed rot and seedling blight Diplodia maydis, Diplodia leaf spot or leaf streak Stenocarpella macrospora=Diplodia macrospora, Downy mildews:Brown stripe downy mildew Sclerophthora rayssiae, Crazy top downy mildew Sclerophthora macrospora= Sclerospora macrospora, Green ear downy mildew (graminicola downy mildew) Sclerospora graminicola, Java downy mildew Peronosclerospora maydis = Sclerospora maydis, Sorghum downy mildew Peronosclerospora sorghi= Sclerospora sorghi, Spontaneum downy mildew Peronosclerospora spontanea=Sclerospora spontanea, Sugarcane downy mildew Peronosclerospora sacchari= Sclerospora sacchari, Dry ear rot (cob, kernel and stalk rot) Nigrospora oryzae, Ear rots, minor Alternaria alternata= A. tenuis, Aspergillus glaucus, A. niger, Aspergillus spp., Botrytis cinerea, Doratomyces stemonitis=Cephalotrichum stemonitis, Fusarium culmorum,Gonatobotrys simplex , Pithomyces maydicus, Rhizopus microspores, R. stolonifer= R.nigricans, Scopulariopsis brumptii, Ergot Claviceps gigantean, Eyespot Aureobasidium zeae= Kabatiella zeae, Fusarium ear and stalk rot Fusarium subglutinans = F. moniliforme, Fusarium kernel, root and stalk rot, seed rot and seedling blight Fusarium moniliforme, Fusarium stalk rot, seedling root rot Fusarium avenaceum, Gibberella ear and stalk rot Gibberella zeae, Gray ear rot Botryosphaeria zeae= Physalospora zeae, Gray leaf spot (Cercospora leaf spot) Cercospora sorghi= C. sorghi, C. zeae-maydis, Helminthosporium root rot Exserohilum pedicellatum=Helminthosporium pedicellatum, Hormodendrum ear rot (Cladosporium rot) Cladosporium cladosporioides = Hormodendrum cladosporioides, C. herbarum, Hyalothyridium leaf spot Hyalothyridium maydis Late wilt Cephalosporium maydis, Leaf spots, minor Alternaria alternate, Ascochyta maydis, A. tritici, A. zeicola, Bipolaris victoriae= Helminthosporium victoriae,Epicoccum nigrum, Exserohilum prolatum= Drechslera prolata, Graphium penicillioides, Leptosphaeria maydis, Leptothyrium zeae, Ophiosphaerella herpotricha, Paraphaeosphaeria michotii, Phoma sp., Septoria zeae, S. zeicola, S. zeina, Northern corn leaf blight (white blast, crown stalk rot, stripe) Setosphaeria turcica, Helminthosporium turcicum, Northern corn leaf spot, Helminthosporium ear rot Cochliobolus carbonum (anamorph: Bipolaris zeicola), Penicillium ear rot (blue eye, blue mold) Penicillium spp., P. chrysogenum, P. expansum, P. oxalicum, Phaeocytostroma stalk rot and root rot Phaeocytostroma ambiguum= Phaeocytosporella zeae, Phaeosphaeria leaf spot Phaeosphaeria maydis =Sphaerulina maydis, Physalospora ear rot (Botryosphaeria ear rot) Botryosphaeria festucae= Physalospora zeicola, Pyrenochaeta stalk rot and root rot Phoma terrestris= Pyrenochaeta terrestris, Pythium root rot Pythium spp., P. arrhenomanes, P. graminicola, Pythium stalk rot, Red kernel disease (ear mold, leaf and seed rot) Epicoccum nigrum, Rhizoctonia ear rot (sclerotial rot), Rhizoctonia zeae, Rhizoctonia root rot and stalk rot Rhizoctonia solani, Root rots, minor Alternaria alternate, Cercospora sorghi, Dictochaeta fertilis, Fusarium acuminatum F. equiseti, F. oxysporum , F. pallidoroseum, F. poae, F. roseum, G. cyanogena, F. sulphureum, Microdochium bolleyi, Mucor sp., Periconia circinata, Phytophthora cactorum, P. drechslerai, P. nicotianae, Rhizopus arrhizus , Rostratum leaf spot (Helminthosporium leaf disease, ear and stalk rot) Setosphaeria rostrata= Helminthosporium rostratum Drechs, Rust, common corn Puccinia sorghi, Rust, southern corn, Puccinia polysora, Rust, tropical corn Physopella pallescens, Sclerotium ear rot* (southern blight) Sclerotium rolfsii, Seed rot-seedling blight Bipolaris sorokiniana, B. zeicola=Helminthosporium carbonum , Diplodia maydis , Exserohilum pedicillatum, Fusarium avenaceum , F. culmorum, F. moniliforme, F. graminearum, Macrophomina phaseolina, Penicillium spp., Phomopsis sp., Pythium spp., Rhizoctonia solani, R. zeae, Sclerotium rolfsii, Spicaria sp., Selenophoma leaf spot Selenophoma sp., Sheath rot Gaeumannomyces graminis, Shuck rot Myrothecium gramineum, Silage mold Monascus purpureus, Smut, common Ustilago zeae= U. maydis, Smut, false Ustilaginoidea virens, Smut, head Sphacelotheca reiliana= Sporisorium holci-sorghi, Southern corn leaf blight and stalk rot Cochliobolus heterostrophus (anamorph: Bipolaris maydis), Southern leaf spot Stenocarpella macrospora = Diplodia macrospora Earle, Stalk rots, minor Cercospora sorghi, Fusarium episphaeria, F. merismoides, F. oxysporum, F. poae, F. roseum , F. solani , F. tricinctum, White ear rot, root and stalk rot Stenocarpella maydis= Diplodia zeae, Yellow leaf blight Ascochyta ischaemi , Phyllosticta maydis, teleomorph: Mycosphaerella zeae-maydis, Zonate leaf spot Gloeocercospora sorghi, smut (Ustilago maydis), Gomaha blight (Cochliobolus heterostrophus), southern rust (Puccinia polysora), gray leaf spot disease (Cercospora zeae-maydis), seedling wilt (Rhizoctonia solani).

Diseases of Pearl Millet: Bipolaris leaf spot Bipolaris setariae, Cercospora leaf spot Cercospora penniseti,Curvularia leaf spot Curvularia penniseti, Downy mildew Sclerospora graminicola, Plasmopara penniseti, Drechslera leaf spot Drechslera dematioidea, Ergot Claviceps fusiformis, Head mold Various fungi Myrothecium leaf spot Myrothecium roridum, Phyllosticta leaf blight Phyllosticta penicillariae, Pyricularia leaf spot Pyricularia grisea, Rhizoctonia blight Rhizoctonia solani, Rhizoctonia zeae, Rust Puccinia substriata, Seedling blight, Smut Moesziomyces penicillariae, Southern blight Sclerotium rolfsii, Top rot Fusarium moniliforme, Zonate leaf spot Gleocercospora sorghi.

Diseases of Sorghum: Acremonium wilt Acremonium strictum = Cephalosporium acremonium, Anthracnose (foliar, head, root and stalk rot) Colletotrichum graminicola (teleomorph: Glomerella graminicola), Charcoal rot Macrophomina phaseolina, Crazy top downy mildew Sclerophthora macrospora, = Sclerospora macrospora, Damping-off and seed rot Aspergillus spp., Exserohilium sp., Fusarium spp., Penicillium spp., Pythium spp.,Rhizoctonia spp., and other species, Ergot Sphacelia sorghi, (teleomorph: Claviceps sorghi), Fusarium head blight, root and stalk rot Fusarium moniliforme (teleomorph: Gibberella fujikuroi), Fusarium spp. Grain storage mold Aspergillus spp., Penicillium spp. and other species, Gray leaf spot Cercospora sorghi, Latter leaf spot Cercospora fusimaculans, Leaf blight Setosphaeria turcica, Milo disease (Periconia root rot) Periconia circinata, Oval leaf spot Ramulispora sorghicola, Twisted top Gibberella fujikuroe var subglutinans, (anamorph: Fusarium moniliforme var. subglutinans), Pythium root rot Pythium spp., P. graminicola, Rough leaf spot Ascochyta sorghi, Rust Puccinia purpurea, Seedling blight and seed rot Colletotrichum graminicola, Exserohilum turcicum, Fusarium moniforme, Pythium spp., Smut, covered kernel Sporisorium sorghi=Sphacelotheca sorghi, Smut, head Sphacelotheca reiliana, Smut, loose kernel Sporisorium cruentum, Sooty stripe Ramulispora sorghi, Sorghum downy mildew Peronosclerospora sorghi= Sclerospora sorghi, Zonate leaf spot and sheath blight Gloeocercospora sorghi.

Diseases of Soybeans: Alternaria leaf spot Alternaria spp. Anthracnose Colletotrichum truncatum, Black root rot Thielaviopsis basicola,Brown spot Septoria glycines,Brown stem rot Cephalosporium gregat, Charcoal rot Macrophomina phaseolina, Choanephora leaf blight Choanephora infundibulifera,Damping-off Rhizoctonia solani, Pythium aphanidermatum,P. ultimum, Downy mildew Peronospora manshurica, Drechslera blight Drechslera glycines, Frogeye leaf spot Cercospora sojina, Fusarium root rot Fusarium spp., Phomopsis seed decay Phomopsis spp., Phytophthora root and stem rot Phytophthora sojae, Pod and stem blight Diaporthe phaseolorum, Powdery mildew Microsphaera diffusa, Pythium rot, Rhizoctonia aerial blight Rhizoctonia solani, Rhioctonia root and stem rot Rhizoctonia solani, Rust Phakopsora pachyrhizi, Sclerotinia stem rot Sclerotinia sclerotiorum, Southern blight (damping-off and stem rot) = Sclerotium blight Sclerotium rolfsii, Stemphylium leaf blight Stemphylium botryosum, Sudden death syndrome Fusarium solani.

Diseases of Peanut: Alternaria leaf blight Alternaria tenuissima, Alternaria leaf spot Alternaria arachidis,Alternaria alternate, Anthracnose Colletotrichum arachidis,Aspergillus crown rot Aspergillus niger, Botrytis blight Botrytis cinerea, Charcoal rot and Macrophomina leaf spot Macrophomina phaseolina,Choanephora leaf spot Choanephora spp. Collar rot Diplodia gossypina, Colletotrichum leaf spot Colletotrichum gloeosporioides,Cylindrocladium black rot & leaf spot Cylindrocladium crotalariae, Damping-off, Aspergillus flavus, A. niger, Fusarium spp., Pythium spp., Rhizoctonia spp., Rhizopus spp., Drechslera leaf spot Bipolaris spicifera=Drechslera spicifera,Fusarium peg and root rot Fusarium spp., Fusarium wilt Fusarium oxysporum, Leaf spot, early Cercospora arachidicola (teleomorph: Mycosphaerella arachidis), Leaf spot, late Paeoisariopsis personata= Cercosporidium personatum, Myrothecium leaf blight Myrothecium roridum, Peanut Smut Thecaphora frezii, Phoma leaf blight Phoma microspora, Phomopsis foliar blight Phomopsis phaseoli, Phomopsis leaf spot, Pod rot (pod breakdown) Fusarium equiseti, F. solani, Pythium myriotylum, Rhizoctonia solani, Powdery mildew Oidium arachidis, Pythium peg and root rot, Pythium myriotylum,P. aphanidermatum, P. debaryanum, P. irregulare, P. ultimum, Pythium wilt Pythium myriotylum, Rhizoctonia foliar blight, peg and root rot Rhizoctonia solani, Rust Puccinia arachidis, Sclerotinia blight Sclerotinia minor, S. sclerotiorum, Stem rot (southern blight) Sclerotium rolfsii, Verticillium wilt Verticillium albo-atrum, V. dahliae, Web blotch (net blotch) Phoma arachidicola= Ascochyta adzamethica, Yellow mold Aspergillus flavus, A. parasiticus, Southern blight (Sclerotium rolfsii), Rhizoctonia root rot, Fusarium root rot.

Diseases of Sunflower: Alternaria leaf blight, stem spot and head rot Alternaria alternate, A. tenuis, A. helianthi, Botrytis head rot (gray mold) Botrytis cinerea, Charcoal rot Macrophomina phaseolina= Sclerotium bataticola=Rhizoctonia bataticola, Downy mildew Plasmopara halstedii, Fusarium stalk rot Fusarium equiseti, F. solani, Fusarium tabacinum, Fusarium wilt Fusarium moniliforme, F. oxysporum,Myrothecium leaf and stem spot Myrothecium roridum,Phoma black stem Phoma macdonaldii, Phomopsis brown stem canker Phomopsis spp., P. helianthi, Phymatotrichum root rot (cotton root rot) Phymatotrichopsis omnivora=Phymatotrichum omnivorum, Phytophthora stem rot Phytophthora spp., Powdery mildew Erysiphe cichoracearum (anamorph: Oidium asteris-punicei), Leveillula compositarum, L. taurica (anamorph: Oidiopsis sicula), Sphaerotheca fuliginea, Pythium seedling blight and root rot Pythium spp., Rhizoctonia seedling blight Rhizoctonia solani, Rhizopus head rot Rhizopus arrhizus, R. nodosus, R. microspores, R. stolonifer, R. nigricans, Rust Puccinia helianthi, Uromyces junci, Sclerotinia basal stalk rot and wilt, mid-stalk rot, head rot Sclerotinia sclerotiorum, Sclerotinia minor, Sclerotium rolfsii, Septoria leaf spot Septoria helianthi , Verticillium wilt Verticillium albo-atrum, White rust, Albugo tragopogonis, Yellow rust Coleosporium helianthi (Schwein.)

Diseases of Mustard, Canola & Rape seed: Alternaria black spot Alternaria brassicae, A. brassicicola, A. raphani, Anthracnose Colletotrichum gloeosporioides (teleomorph: Glomerella cingulata), Black leg (leaf, root and stem rot) Leptosphaeria maculans (anamorph: Phoma lingam), Black root Aphanomyces raphani, Cercospora leaf spot Cercospora brassicicola, Clubroot Plasmodiophora brassicae, Damping-off Fusarium spp., Rhizoctonia solani, Downy mildew Peronospora parasitica, Head rot Rhizoctonia solani, Leaf spot Myrothecium roridum Phyllosticta brassicae, Powdery mildew Erysiphe polygoni, Sclerotinia stem rot Sclerotinia sclerotiorum White rust Albugo candida, White leaf spot Pseudocercosporella capsellae, Black mold rot Rhizopus stolonifer, Black root Aphanomyces raphani, Cercospora leaf spot Cercospora brassicicola, Clubroot Plasmodiophora brassicae, Downy mildew Peronospora parasitica, Fusarium wilt Fusarium oxysporum, Gray mold Botrytis cinerea, Head rot Rhizoctonia solani, Light leaf spot Pyrenopeziza brassicae, Pod rot Alternaria alternate, Cladosporium sp., Ring spot Mycosphaerella brassicicola, Root rot Alternaria alternate, Fusarium spp., Macrophomina phaseolina, Phymatotrichopsis omnivore, Phytophthora megasperma, Pythium debaryanum, Rhizoctonia solani, Sclerotium rolfsii, Sclerotinia stem rot Sclerotinia sclerotiorum, Seed rot, damping-off Alternaria spp., Fusarium spp., Gliocladium roseum, Nectria ochroleuca, Pythium spp., Rhizoctonia solani, Rhizopus stolonifer, Verticillium wilt Verticillium longisporum, White leaf spot = grey stem Pseudocercosporella capsellae = Cercosporella brassicae, White rust = staghead Albugo candida.

Diseases of Cotton: Anthracnose Glomerella gossypii, Colletotrichum gossypii, Areolate mildew Ramularia gossypii = Cercosporella gossypii, Ascochyta blight Ascochyta gossypii, Black root rot Thielaviopsis basicola, Boll rot Ascochyta gossypii , Colletotrichum gossypii , Southworth Glomerella gossypii, Fusarium spp., Phytophthora spp., Rhizoctonia solani, Charcoal rot Macrophomina phaseolina, Fusarium wilt Fusarium oxysporum, Leaf spot Alternaria macrospora, A. alternate, Cercospora gossypina, Mycosphaerella gossypina, Cochliobolus spicifer, Bipolaris spicifera = Curvularia spicifera, Myrothecium roridum, Rhizoctonia solani, Stemphylium solani, Lint contamination Aspergillus flavus, Nematospora spp., Nigrospora oryzae, Phymatotrichum root rot = cotton root rot Phymatotrichopsis omnivore = Phymatotrichum omnivorum, Powdery mildew Leveillula taurica, Oidiopsis sicula = Oidiopsis gossypii, Rust Cotton rust Puccinia schedonnardi, Sclerotium stem and root rot = southern blight Sclerotium rolfsii, Seedling disease complex Colletotrichum gossypii, Fusarium spp., Pythium spp., Rhizoctonia solani, Thielaviopsis basicola, Stem canker Phoma exigua, Verticillium wilt Verticillium dahliae, Rhizoctonia seedling damping-off disease caused by Rhizoctonia solani, wilt by Fusarium oxysporum.

Diseases of Bean (Phaseolus vulgaris L.): Alternaria leaf and pod spot Alternaria alternate, Alternaria brassicae, Alternaria brassicicola, Angular leaf spot Phaeoisariopsis griseola, Anthracnose Colletotrichum lindemuthianum,Aphanomyces root and hypocotyl rot Aphanomyces euteiches, Ashy stem blight (charcoal rot) Macrophomina phaseolina, Black node disease (Ascochyta leaf spot) Phoma exigua= Ascochyta phaseolorum, Black root rot Thielaviopsis basicola, Cercospora leaf spot and blotch Pseudocercospora cruenta, Mycosphaerella cruenta, Cercospora canescens, Cercospora phaseoli, Cercospora caracallae, Chaetoseptoria leaf spot Chaetoseptoria wellmanii, Damping-off and stem rot, Rhizoctonia Rhizoctonia solani, Damping-off, Pythium spp., Diaporthe pod blight Diaporthe phaseolorum , Downy mildew Phytophthora nicotianae, Entyloma leaf smut Entyloma petunia, Floury leaf spot Mycovellosiella phaseoli, Fusarium root rot Fusarium solani, Gray leaf spot Cercospora vanderysti, Gray mold Botrytis cinerea, Botryotinia fuckeliana, Pink pod rot Trichothecium roseum, Powdery mildew Erysiphe polygoni, Rust Uromyces appendiculatus, Scab Elsinoe phaseoli, Soybean rust Phakopsora pachyrhizi {Asian Soybean Rust}.

Diseases of Chickpea: Acrophialophora wilt Acrophialophora fusispora, Alternaria blight Alternaria alternate, Alternaria tenuissima, Aphanomyces root rot Aphanomyces euteiches, Ascochyta blight Ascochyta rabiei, Black root rot Fusarium solani, Black streak root rot Thielaviopsis basicola, Botrytis gray mold Botrytis cinerea , Collar rot Sclerotium rolfsii, Colletotrichum blight Colletotrichum capsici, Colletotrichum dematium, Cylindrocladium root rot Cylindrocladium clavatum, Downy mildew Peronospora sp., Dry root rot Macrophomina phaseolina, Fusarium root rot Fusarium acuminatum, Fusarium arthrosporioides, Fusarium avenaceum, Fusarium equiseti, Fusarium solani, Fusarium wilt Fusarium oxysporum, Neocosmospora root rot Neocosmospora vasinfecta, Phoma blight Phoma medicaginis, Phytophthora root rot Phytophthora citrophthora, Phytophthora cryptogea,Phytophthora drechslera, Phytophthora megasperma, Pleospora leaf spot Pleospora herbarum, Powdery mildewLeveillula taurica, Erysiphe sp., Rust Uromyces ciceris-arietini, Uromyces striatus, Sclerotinia stem rot Sclerotinia sclerotiorum, Sclerotinia trifoliorum, Scopulariopsis leaf spot Scopulariopsis brevicaulis, Seedling or seed rot Aspergillus flavus, Trichothecium roseum, Stemphylium blight Stemphylium sarciniforme, Verticillium wilt Verticillium albo-atrum, Verticillium dahliae, Wet root rot Rhizoctonia solani.

Diseases of Lentil & Peas: Alternaria blight Alternaria alternate, Anthracnose Colletotrichum lindemuthianum, Colletotrichum truncatum, Aphanomyces root rot Aphanomyces euteiches, Ascochyta blight Ascochyta fabae, Black root rot Fusarium solani, Black streak root rot Thielaviopsis basicola, Botrytis gray mold Botrytis cinerea, Cercospora leaf spot Cercospora cruenta, Cercospora lensii, Cercospora zonata, Collar rot Sclerotium rolfsii, Cylindrosporium leaf spot and stem canker Cylindrosporium sp., Downy mildew Peronospora lentis, Peronospora viciae, Dry root rot Macrophomina phaseolina= Rhizoctonia bataticola, Fusarium wilt Fusarium oxysporum, Helminthosporium leaf spot Helminthosporium sp., Leaf rot Choanephora sp., Leaf yellowing Cladosporium herbarum, Phoma leaf spot Phoma medicaginis, Powdery mildew Erysiphe pisi= Erysiphe polygoni, Leveillula taurica= Leveillula leguminosarum, Pythium root and seedling rot Pythium aphanidermatum, Pythium ultimum, Rust Uromyces craccae, Uromyces viciae-fabae, Sclerotinia stem rot Sclerotinia sclerotiorum, Stemphylium blight Stemphylium botryosum, Stemphylium sarciniforme.

Diseases of Pigeonpea: Alternaria blight Alternaria alternate, Alternaria tenuissima, Anthracnose Colletotrichum cajani, Colletotrichum truncatum, Armillaria root rot Armillaria mellea, Botryodiplodia blight Lasiodiplodia theobromae= Botryodiplodia theobromae, Botrytis gray mold Botrytis cinerea, Cercoseptoria leaf spot Cercoseptoria cajanicola, Cercospora leaf spot Mycovellosiella cajani= Cercospora cajani, Cercospora indica, Cercospora instabilis, Cercospora thirumalacharii, Cladosporium leaf blight Cladosporium oxysporum, Collar rot Sclerotium rolfsii= Corticium rolfsii, Colletotrichum stem canker Colletotrichum crassipes, Damping-off Pythium aphanidermatum, Dieback and stem canker Colletotrichum capsici, Diplodia stem canker Diplodia cajani, Dry root rot Macrophomina phaseolina, Fusarium leaf blight Fusarium pallidoroseum, Fusarium seedling rot Fusarium avenaceum, Fusarium equiseti, Fusarium wilt Fusarium udum, Halo blight Pseudomonas syringae, Macrophomina stem canker Macrophomina phaseolina, Myrothecium leaf spot Myrothecium gramineum, Periconia leaf spot Periconia byssoides, Phaeoisariopsis leaf spot Phaeoisariopsis griseola, Phoma stem canker Phoma cajani, Phyllosticta leaf spot Phyllosticta cajani, Phytophthora root rot and stem blight Phytophthora drechslera, Powdery mildew Leveillula taurica, Rust Uredo cajani, Sclerotinia stem rot Sclerotinia sclerotiorum, Seedling or seed rot Aspergillus flavus, Aspergillus niger, Wet root rot Rhizoctonia solani.

Diseases of Sugarcane: Black rot Ceratocystis adipose, Black stripe Cercospora atrofiliformis, Brown spot Cercospora longipes, Downy mildew Peronosclerospora sacchari, Fusarium sett and stem rot Gibberella fujikuroi, Leaf blast Didymosphaeria taiwanensis, Leaf blight Leptosphaeria taiwanensis,Red leaf spot (purple spot) Dimeriella sacchari, Red rot Glomerella tucumanensis, Red rot of leaf sheath and sprout rot Athelia rolfsii, Rhizoctonia sheath and shoot rot Rhizoctonia solani, Rust, common Puccinia melanocephala, Seedling blight Alternaria alternate, Bipolaris sacchari, Cochliobolus hawaiiensis, Curvularia lunata, Setosphaeria rostrata, Drechslera halodes, Sheath rot Cytospora sacchari, Smut Ustilago scitaminea, Wilt Fusarium sacchari, Zonate leaf spot Gloeocercospora sorghi.

Diseases of Carrot: Alternaria leaf blight Alternaria dauci, Black root rot Thielaviopsis basicola , Black rot (black carrot root dieback) Alternaria radicina=Stemphylium radicinum , Brown rot (Phoma disease) Leptosphaeria libanotis, Phoma rostrupii, Cavity spot Pythium spp., Pythium violae, Rhizoctonia spp., Cercospora leaf spot Cercospora carotae, Cottony rot Sclerotinia minor, Crown rot Rhizoctonia spp., Downy mildew Plasmopara crustosa, Leaf spot Ramularia spp., Powdery mildew Erysiphe heraclei, E. polygoni, E. umbelliferaum.

Diseases of Cucurbits: Alternaria leaf blight Alternaria cucumerina, Alternaria leaf spot Alternaria alternata f. sp. Cucurbitae, Anthracnose (stem, leaf and fruit) Colletotrichum orbiculare, Belly rot Rhizoctonia solani, Black root rot Thielaviopsis basicola, Blue mold rot Penicillium spp., P. digitatum, Cephalosporium root and hypocotyl rot, stem streak and dieback Acremonium spp.= Cephalosporium spp., Cercospora leaf spot Cercospora citrullina, Charcoal rot (vine decline and fruit rot) Macrophomina phaseolina, Choanephora fruit rot Choanephora cucurbitarum, Crown and foot rot Fusarium solani, Damping-off Acremonium spp., Fusarium spp., F. equiseti, Phytophthora sp., Pythium spp., Rhizoctonia solani, Downy mildew Pseudoperonospora cubensis, Fusarium fruit rot Fusarium equiseti,Fusarium wilt Fusarium oxysporum, Gray mold Botrytis cinerea, Gummy stem blight (vine decline) Didymella bryoniae= Mycosphaerella melonis, Powdery mildew Sphaerotheca fuliginea, Erysiphe cichoracearum, Pythium fruit rot (cottony leak) Pythium spp., Rhizopus soft rot (fruit) Rhizopus stolonifer, Scab/gummosis Cladosporium cucumerinum, Sclerotinia stem rot Sclerotinia sclerotiorum, Septoria leaf blight Septoria cucurbitacearum, Sudden wilt Pythium aphanidermatum, Ulocladium leaf spot Ulocladium consortiale, Verticillium wilt Verticillium albo-atrum, V. dahliae, Web blight Rhizoctonia solani, anthracnose (Colletotrichum lagenarium), powdery mildew (Sphaerotheca fuliginea), vine blight (Mycosphaerella melonis), Fusarium disease (Fusarium oxysporum), downy mildew (Pseudoperonospora cubensis), late blight (Phytophthora sp.), seedling blight (Pythium sp).

Diseases of Onion and Garlic: Black mold Aspergillus niger, Blue mold Penicillium aurantiogriseum, = Penicillium cyclopium, Penicillium citrinum, Penicillium digitatum, Penicillium expansum, Penicillium hirsutum, Penicillium funiculosum, Penicillium oxalicum, Botrytis brown stain Botrytis cinerea, Botryotinia fuckeliana, Botrytis leaf blight Botrytis squamosa, Botrytis rot of garlic Botrytis porri , Botryotinia porri, Cercospora leaf spot Cercospora duddiae, Charcoal rot Macrophomina phaseolina =Sclerotium bataticola, Diplodia stain Lasiodiplodia theobromae = Diplodia natalensis= Botryodiplodia theobromae, Downy mildew Peronospora destructor, Flower and capsule blight Botrytis aclada, Botrytis allii, Botrytis squamosa, Botryotinia squamosa, Botrytis cinerea, Botryotinia fuckeliana, Fusarium basal rot Fusarium oxysporum,Fusarium bulb rot of onion and garlic Fusarium proliferatum, Fusarium damping-off Fusarium oxysporum, Fusarium verticillioides= Fusarium moniliforme, Fusarium subglutinans, Fusarium proliferatum= Cephalosporium proliferatum, Fusarium solani, Fusarium equiseti, Fusarium tricinctum, Leaf blotch Cladosporium allii-cepae, Mycosphaerella allii-cepae, Mushy rot Rhizopus microspores, Rhizopus stolonifer, Mycelial neck rot Botrytis byssoidea, Botryotinia allii, Neck rot Botrytis aclada, Botrytis allii, Penicillium decay of garlic Penicillium hirsutum, Pink root Phoma terrestris, Powdery mildew Leveillula taurica, Oidiopsis sicula Scalia, Purple blotch Alternaria porri, Pythium seed rot and damping-off Pythium irregulare, Pythium coloratum, Pythium graminicola, Pythium mamillatum, Pythium paroecandrum, Pythium rostratum, Pythium spinosum, Pythium sylvaticum , Pythium torulosum, Pythium ultimum, Pythium vexans, Rhizoctonia seed rot and seedling disease Rhizoctonia solani, Rust Puccinia allii, Scape blight Botrytis aclada, Botrytis allii, Botrytis porri, Botrytis squamosa, Sclerotinia rot Sclerotinia sclerotiorum, Smudge Colletotrichum circinans, Smut Urocystis colchici, Stemphylium leaf blight and stalk rot Stemphylium vesicarium, White rot Sclerotium cepivorum.

Diseases of Pepper (Capsicum spp.): Anthracnose Colletotrichum gloeosporioides, C. capsici, Glomerella cingulata, Cercospora (frogeye) leaf spot Cercospora capsici, Charcoal rot Macrophomina phaseolina, Choanephora blight (wet rot) Choanephora cucurbitarum, Damping-off and root rot Rhizoctonia solani, Phytophthora spp., Fusarium spp., Pythium spp., Downy mildew Peronospora tabacina, Fusarium stem rot Fusarium solani, Fusarium wilt Fusarium oxysporum f. sp. Capsici,Gray leaf spot Stemphylium solani, Gray mold Botrytis cinerea, Phytophthora blight Phytophthora capsici, Powdery mildew Oidiopsis sicula, Leveillula taurica, Southern blight Sclerotium rolfsii, Verticillium wilt erticillium dahliae, White mold Sclerotinia sclerotiorum.

Diseases of Potato (Solanum tuberosum L.): Brown spot and Black pit Alternaria alternate, Cercospora leaf blotch Mycovellosiella concors= Cercospora concors, Cercospora solani, Early blight Alternaria solani, Gray mold Botrytis cinerea, Late blight Phytophthora infestans, Phoma leaf spot Phoma andigena, Powdery mildew Erysiphe cichoracearum, Powdery scab Spongospora subterranean, Rhizoctonia canker and black scurf Rhizoctonia solani, Septoria leaf spot Septoria lycopersici, Silver scurf Helminthosporium solani, Verticillium wilt Verticillium albo-atrum, Scarlet rot (Phytophthora erythroseptica).

Diseases of Tomato (Solanum lycopersicum L.) : Alternaria stem canker Alternaria alternate f. sp. Lycopersici, , Anthracnose Colletotrichum coccodes, Black mold Alternaria alternate, Buckeye rot Phytophthora capsici, Charcoal rot Macrophomina phaseolina, Didymella stem rot Didymella lycopersici, Early blight Alternaria tomatophila, Fusarium foot rot Fusarium solani, Gray leaf spot Stemphylium botryosum f. sp. Lycopersici,Gray mold Botrytis cinerea, Gray mold rot (Botrytis fruit rot) Botrytis cinerea, Late blight Phytophthora infestans,Phoma rot Phoma destructiva var. destructive, Phytophthora root rot Phytophthora capsici, Powdery mildew Leveillula taurica, Pythium damping-off and stem rot Pythium aphanidermatum, P. debaryanum, P. ultimum, Rhizoctonia damping-off, root rot, stem rot, sem canker, foliar blight, fruit rot Rhizoctonia solani, Septoria leaf spot Septoria lycopersici, Verticillium wilt Verticillium albo-atrum, V. dahliae, White mold Sclerotinia minor, S. sclerotiorum, early blight (Alternaria solani), leaf mold disease (Cladosporium fulvum), late blight (Phytophthora infestans).

Diseases of Apple: Alternaria blotch Alternaria mali, Alternaria rot Alternaria alternate, American brown rot Monilinia fructicola, Anthracnose canker and bull's-eye rot Cryptosporiopsis curvispora, Apple scab Venturia inaequalis, Apple ring rot and canker Botryosphaeria berengeriana, Armillaria root rot = shoestring root rot Armillaria mellea, Bitter rot Glomerella cingulata, Black pox Helminthosporium papulosum, Black root rot Xylaria mali, Black rot, frogeye leafspot and canker Botryosphaeria obtuse, Blister canker = nailhead canker Biscogniauxia marginata, Blue mold Penicillium spp., Brooks fruit spot Mycosphaerella pomi, Brown rot blossom blight and spur infection Monilinia laxa, (Aderhold & Ruhland) Honey, Clitocybe root rot Armillaria tabescens = Clitocybe tabescens, Diaporthe canker Diaporthe tanakae, Phomopsis tanakae, Diplodia canker Botryosphaeria stevensii, Diplodia mutila, Fruit blotch, leaf spot and twig canker Phyllosticta solitaria, Glomerella leaf spot Glomerella cingulata, Colletotrichum gloeosporioides, Gray mold rot = dry eye rot, blossom-end rot Botrytis cinerea, Leptosphaeria canker and fruit rot Diapleella coniothyrium = Leptosphaeria coniothyrium, Leucostoma canker and dieback Leucostoma cincta, Cytospora cincta, Marssonina blotch Diplocarpon mali, Marssonina coronaria, Moldy core and core rot Alternaria spp., Cladosporium spp., Coniothyrium sp., Epicoccum spp., Pleospora herbarum, Stemphylium spp., Ulocladium spp., Monilia leaf blight Monilinia mali, Monochaetia twig canker Seiridium unicorne =Monochaetia mali, Perennial canker Neofabrae perennans, Cryptosporiopsis perennans, Phomopsis canker, fruit decay and rough bark Phomopsis mali, Phymatotrichum root rot = cotton root rot Phymatotrichopsis omnivore, Phytophthora crown, collar and root rot = sprinkler rot Phytophthora spp., Pink mold rot Trichothecium roseum = Cephalothecium roseum, Powdery mildew Podosphaera leucotricha, blossom disease (Monilinia mali), canker (Valsa ceratosperma), powdery mildew (Podosphaera leucotricha), speckled leaf fall disease (Alternaria alternata apple pathotype), scab (Venturia inaequalis), anthracnose (Colletotrichum acutatum) , plague (Phytophtora cactorum), brown spot (Diplocarpon mali), early blight (Botryosphaeria berengeriana), Marssonia leaf blotch.

Diseases of Banana: Anthracnose Colletotrichum musae, Armillaria corn rot Armillaria mellea,Black leaf streak (black Sigatoka) Mycosphaerella fijiensis, Black root rot Rosellinia bunodes, Brown spot Cercospora hayi, Ceratocystis fruit rot Ceratocystis paradoxa, Cigar-end Verticillium theobromae, Crown rot Fusarium pallidoroseum, Colletotrichum musae, Verticillium theobromae, Fusarium spp., Cylindrocladium root rot Cylindrocladium spp., Eyespot Dreschslera gigantean, Fruit rot Botryosphaeria ribis, Fungal root-rot Fusarium solani, Fusarium wilt (Panama disease) Fusarium oxysporum, Leaf spot Curvularia eragrostidis, Drechslera musae-sapientum, Leptosphaeria musarum, Pestalotiopsis disseminate, Ceratocystis paradoxa, Sigatoka (yellow Sigatoka) Mycosphaerella musicola.

Diseases of Citrus (Citrus spp.): Alternaria brown spot Alternaria alternate, Anthracnose = wither-tip Glomerella cingulata, Colletotrichum gloeosporioides, Black mold rot Aspergillus niger, Botrytis blossom and twig blight, gummosis Botrytis cinerea, Brown rot (fruit) Phytophthora citricola, P. citrophthora, Diplodia gummosis and stem-end rot Lasiodiplodia theobromae = Botryodiplodia theobromae, Leaf spot Mycosphaerella horii, Phytophthora foot rot, gummosis and root rot Phytophthora citrophthora., black spot disease (Diaporthe citri), fruit rot (Penicillium digitatum), Phytophthora disease (Phytophthora parasitica).

Diseases of Grape (Vitis spp.): Alternaria rot Alternaria alternate, Anthracnose and bird's-eye rot Elsinoe ampelina (anamorph: Sphaceloma ampelinum), Berry rots and raisin molds Ascochyta sp., Aspergillus aculeatus, Aspergillus spp., Cladosporium spp., Fusarium spp., Helminthosporium spp., Monilia sp., Stemphylium botryosum, Botrytis bunch rot and blight (Gray mold) Botrytis cinerea (teleomorph: Botryotinia fuckeliana) Cercospora leaf spot (cercosporiose) Phaeoramularia dissiliens = Cercospora sp., Diplodia cane dieback and bunch rot (Cane-tip blight) Lasiodiplodia theobromae =Diplodia natalensis, Downy mildew Plasmopara viticola, Powdery mildew Uncinula necator, Wood rot (decay) Cephalosporium spp., Phellinus igniarius, Stereum hirsutum, evening rot (Glomerella cingulata), powdery mildew (Uncinula necator), rust (Phakopsora ampelopsidis), black lot disease (Guignardia bidwellii), downy mildew (Plasmopara viticola).

Diseases of Mango (Mangifera indica L.): Alternaria leaf spots Alternaria alternate, Anthracnose Colletotrichum gloeosporioides, Black mold rot Aspergillus niger, Black rot Ceratocystis paradoxa, Blossom blight Botrytis cinerea, Branch canker Botryosphaeria ribis, Fusicoccum sp., Ceratocystis wilt Ceratocystis fimbriata, Dieback Botryosphaeria disrupta, Fruit rot Alternaria alternate, Phytophthora nicotianae, Leaf blight Bipolaris hawaiiensis, Leaf spot Curvularia lunata, Leptosphaeria sp., Macrophoma sp., Phaeosphaerella mangiferae, Phoma sorghina, Pseudocercospora mali, Septoria sp., Verticillium lecanii. Mango malformation Fusarium subglutinans, Powdery mildew Erysiphe cichoracearum, Stem canker Phoma sp., Stem end rot Botryosphaeria rhodina, Twig blight Diaporthe spp.

Diseases of Papaya: Alternaria fruit spot Alternaria alternate, Angular leaf spot Leveillula taurica, Anthracnose Colletotrichum gloeosporioides, Black spot Asperisporium caricae, Cercospora papaya, Brown spot Corynespora cassiicola, Collar rot Cylindrocladium crotalariae, Damping off/ Root rot Colletotrichum gloeosporioides, Phytophthora palmivora, Pythium aphanidermatum,Pythium debaryanum, Rhizoctonia solani, Dry rot Phoma caricae-papayae, Fruit rot Monilia sp., Fruit spot Cercospora mamaonis, Fusarium fruit rot Fusarium solani, Greasy spot Corynespora cassiicola, Leaf spot Alternaria sp., Cercospora mamaonis, Choanephora cucurbitarum, Curvularia carica-papayae, Powdery mildew Erysiphe cichoracearum, Oidium caricae, Rhizopus soft rot Rhizopus stolonifer.

The lists of crops which can be protected by the present formulation are Cotton (Gossypium spp.), Jute (Corchorus oliotorus), Paddy (Oryza sativa), Wheat (Triticum aestavum), Barley (Hordeum vulgare), Maize (Zea mays), Sorghum (Sorghum bicolor), Ragi (Eleusine coracana), Pearl millet (Pennisetum glaucum), Sugarcane (Saccharum officinarum) , Sugarbeet (Beta vulgaris), Soybean (Glycin max), Peanut (Arachis hypogaea), Sunflower (Helianthus annuus) , Mustard (Brassica juncea), Rape seed (Brassica napus), Linseed (Linum usitatissimum), Sesame (Sesamum indicum), Castor (Ricinus communis), Green gram (Vigna radiate), Black gram (Vigna mungo), Chickpea (Cicer aritinum), Cowpea (Vigna unguiculata), Redgram (Cajanus cajan), Frenchbean (Phaseolus vulgaris), Indian bean (Lablab purpureus), Horse gram (Macrotyloma uniflorum), Field pea (Pisum sativum), Cluster bean (Cyamopsis tetragonoloba), Lentils (Lens culinaris), Brinjal (Solanum melongena), Cabbage (Brassica oleracea var. capitata), Cauliflower (Brassica oleracea var. botrytis), Okra (Abelmoschus esculentus) , Onion (Allium cepa L.), Tomato (Solanum lycopersicun) , Potato (Solanum tuberosum) , Sweet potato (Ipomoea batatas), Chilly (Capsicum annum), Garlic (Allium sativum), Cucumber (Cucumis sativus) and Melons (Cucumis melo), Radish (Raphanus sativus), Carrot (Dacus carota subsp. sativus), Turnip (Brassica rapa subsp rapa), Apple (Melus domestica), Banana (Musa spp.), Citrus groups (Citrus spp.), Grape (Vitis vinifera), Guava (Psidium guajava), Litchi (Litchi chinensis), Mango (Mangifera indica), Papaya (Carica papaya), Pineapple (Ananas comosus), Pomegranate (Punica granatum) , Sapota (Manilkara zapota), Tea (Camellia sinensis), Coffea (Coffea Arabica), Turmeric (Curcuma longa), Ginger (Zingiber officinale), Cumin (Cuminum cyminum), Fenugreek (Trigonella foenum-graecum), Fennel (Foeniculum vulgare), Coriander (Coriandrum sativum), Ajwain (Trachyspermum ammi), Psyllium (Plantago ovate), Black Pepper (Piper nigrum), Stevia (Stevia rebaudiana), Safed musli (Chlorophytum tuberosum), Drum stick (Moringa oleifera), Coconut (Coco nucifera), Mentha (Mentha spp.), Rose (Rosa spp.), Jasmine (Jasminum spp.), Marigold ( Tagetes spp.), Common daisy (Bellis perennis), Dahlia (Dahlia hortnesis).

The formulation of the present invention can be used to control the insects-pests belongs to the order Hemiptera, for example, Apple Mealy bug (Phenococcus aceris), bean aphid (Aphis fabae), black citrus aphid (Toxoptera aurantii), citrus black scale (Saissetia oleae), cabbage aphid (Brevicoryne brassicae, Lipaphis erysimi), citrus red scale (Aonidiella aurantii), citrus mealybug (Planococcus citri), corn leaf aphid (Rhopalosiphum maidis), cotton aphid (Aphis gossypii), cotton leaf hoppers (Amrasca biguttula), cotton mealy bug (Planococcus spp. And Pseudococcus spp.), cotton stainer (Dysdercus suturellus), cotton whitefly (Bemisia tabaci), cowpea aphid (Aphis crassivora), grain aphid (Sitobion avenae), golden glow aphid (Uroleucon spp.), grape mealybug (Pseudococcus maritimus), green peach aphid (Myzus persicae), greenhouse whitefly (Trialeurodes vaporariorum)spp., papaya mealy bug (Pracoccus marginatus), pea aphid (Acyrthosiphon pisum), sugarcane mealybug (Saccharicoccus sacchari), potato aphid (Myzus persicae), potato leaf hopper (Empoasca fabae), rice leafhopper (Nephotettix nigropictus), rice brown plant hopper (Nilaparvata lugen), rice white backedplant hopper ( sweetpotato whitefly ( Bemisia tabaci), tarnished plant bug (Lygus lineolaris), wooly apple aphid (Eriosoma lanigerum), yellow scale (Aonidiella citrine),order Lepidoptera, army worm (Mythimna unipuncta), asiatic rice borer (Chilo suppressalis), bean pod borer (Maruca vitrata), beet armyworm (Spodoptera exigua), black cutworm (Agrotis ipsilon), bollworm (Helicoverpa spp.),cabbage looper (Trichoplusia ni), codling moth (Cydia pomonella), croton caterpillar (Achea janata), diamond backmoth (Plutella xylostella), cabbage worm (Pieris rapae), pink bollworm (Pectinophora gossypiella), sugarcane borer (Diatraea saccharalis), tobacco budworm (Heliothis virescens), tomato fruitworm (Helicoverpa zea), velvet bean caterpillar (Anticarsia gemmatalis), yellow stem borer (SCirpophaga incertulas), spotted bollworm (Earias vittella), rice leaffolder (Cnaphalocrocis medinalis), pink stem borer (Sesamia spp.), tobacco leafeating caterpillar (Spodoptera litura), ; from the order Coleoptera, for example, apple twig borer (Amphicerus spp.), corn root worm (Diabrotica virgifera), cucumber beetle (diabrotica balteata), boll weevil (Anthonomus grandis), grape flea beetle (Altica chalybea), grape root worm (Fidia viticola), grape trunk borer (Clytoleptus albofasciatus), radish flea beetle (Phyllotreta armoraciae), maize weevil (Sitophilus zeamais), northern corn rootworm (Diabrotica barberi), rice water weevil (Lissorhoptrus oryzophilus; from the order Orthoptera, for example, Gryllotalpa spp., Locusta spp., and Schistocerca is spp.; from the order Thysanoptera, for example, Frankliniella spp., Thrips palmi, Thrips tabaci and Scirtothrips dorsalis; from the order Heteroptera, for example, Dysdercus spp., Leptocorisa spp., from the order Hymenoptera, for example, Solenopsis spp.; from the order Diptera, for example, Antherigona soccata, Dacus spp., Liriomyza spp., Melanagromyza spp., from the order Acarina, for example, Aceria mangiferae, Brevipalpus spp., Eriophyes spp., Oligonychus mangiferus, Oligonychus punicae, Panonychus citri, Panonychus ulmi, Polyphagotarsonemus latus, Tarsonemus spp., Tetranychus urticae, Tetranychus cinnabarinus.

Eggplant disease: Phoma blight (Phomopsis vexans), powdery mildew (Erysiphe cichoracearum).

Diseases of cruciferous vegetables: black spot (Alternaria japonica), white spot disease (Cercosporella brassicae), clubroot (Plasmodiophora brassicae), downy mildew (Peronospora parasitica).

Diseases of rapeseed: rot (Sclerotinia sclerotiorum), seedling wilt caused by bacteria of the genus Rhizoctonia (Rhizoctonia solani).

FORMULATIONS OF THE PRESENT INVENTION

Sr. No. Active Ingredient I Active Ingredient II Active Ingredient III A.I.(%) in formulation Formulation per Hectare (g or ml)
1 Mepiquat Chloride Epoxiconazole Azoxystrobin 16.0% 20.0% 32.0% 300
2 Mepiquat Chloride Epoxiconazole Trifloxystrobin 16.0% 20.0% 32.0% 300
3 Mepiquat Chloride Epoxiconazole Pyraclostrobin 16.0% 20.0% 32.0% 300
4 Mepiquat Chloride Epoxiconazole Picoxystrobin 16.0% 20.0% 32.0% 300
5 Mepiquat Chloride Prothioconazole Trifloxystrobin 10% 32.0% 24.0% 375
6 Mepiquat Chloride Prothioconazole Trifloxystrobin 5.0% 16.0% 12.0% 750
7 Mepiquat Chloride Prothioconazole Picoxystrobin 10% 32.0% 24.0% 375
8 Mepiquat Chloride Prothioconazole Picoxystrobin 5.0% 16.0% 12.0% 750
9 Mepiquat Chloride Tebuconazole Trifloxystrobin 10% 35.0% 25% 350
10 Mepiquat Chloride Tebuconazole Trifloxystrobin 5.0% 17.5% 12.5% 700
11 Mepiquat Chloride Tricyclazole Azoxystrobin 10% 40.0% 20% 500
12 Mepiquat Chloride Tricyclazole Azoxystrobin 5.0% 20.0% 10.0% 1000
13 Mepiquat Chloride Tricyclazole Trifloxystrobin 10% 40.0% 20% 500
14 Mepiquat Chloride Tricyclazole Trifloxystrobin 5.0% 20.0% 10.0% 1000
15 Mepiquat Chloride Tricyclazole Picoxystrobin 10.0% 40.0%
13.6% 500
16 Mepiquat Chloride Tricyclazole Picoxystrobin 5.0% 20.3% 6.8% 1000

EXAMPLES:

The synergistic pesticide action of the inventive mixtures can be demonstrated by the experiments below. A synergistic effect exists wherever the action of a combination (ready-mix) of active ingredient is greater than the sum of the action of each of the components alone. Therefore a synergistically effective amount or an effective amount of a synergistic composition or combination is an amount that exhibits greater pesticide activity than the sum of the pesticide activities of the individual components. In the field of agriculture, it is often understood that the term “synergy” is as defined by Colby S.R. in an article entitled “ Calculation of the synergistic and antagonistic responses of herbicide combinations” published in the journal Weeds, 1967, 15, p.20-22, incorporated herein by reference in its entirety. The action expected for a given combination of two active components can be calculated as follows:

Colby’s Formula:

To study the synergistic effect of mepiquat, triazoles and strobilurins, various sets of experiments were conducted in different crops like rice, wheat, peanut, tomato and chilli.

Example 1

The synergistic mixture of Mepiquat, Tebuconazole and Trifloxystrobin was evaluated on rice crop, which has been regarded as one of the most important cereal crops and major food grain contributor to the total world good grain basket. Basmati and fine seeded varieties of rice are well known in the world. Their productivity is influenced by many of the biotic and abiotic stresses. At present, there are very limited strategies are employed to combat both the fungal diseases along with lodging prevention. The objective of the present study was to provide inventive solution to the rice growers to control many fungal diseases and to prevent the lodging which is major concern in basmati and fine seeded varieties of rice in northern part of the country.
Details of Experiment:
a) Experiment design : Randomized Block Design
b) Replication : Three
c) Treatments : Ten
d) Plot size : 50 sq. m.
e) Spacing : 2 seedlings per hill
f) Test Crop & Variety : Rice, PR 1121
g) Time of application : Two spray. 1st at 20, and 2nd at 50 DATP (Days After Transplanting)
h) Spray method : Manually operated knap sack sprayer, using 500 liter per hectare water volume
Evaluation Method:
1) Sheath blight: Observed 20 hills per plot and visually rate the disease by the following rating.
Score Description
0 No Infection
1 Vertical spread of diseases up to 20% of plant height
3 Vertical spread of diseases up to 21-30% of plant height
5 Vertical spread of diseases up to 31-45% of plant height
7 Vertical spread of diseases up to 46-65% of plant height
9 Vertical spread of diseases >66% of plant height

Calculate disease severity as

PDI (%) = Sum of numerical rating x 100 .
Total no. of plants observed x Max. Grade

The percent disease reduction over Untreated Check (UTC) was calculated as below:

% Reduction over UTC = 100 - __(100 x PDI % in Treatment)__
(PDI % in Untreated Check)

2) False smut: Observe all the panicles from 0.25 m2 (50 x 50 cm) area and count no. of smutted grains per 0.25 m2 area from 10 random spots per plot at mature grain stage. Present the data as No. of smutted grains per m2. Then calculate % Disease reduction over UTC as per the formula given above.

3) Grain Discoloration: Observe 100 panicles per plot and count the no. of Healthy & Infected grains per panicles at mature grain stage. Calculate % Grain Discoloration and then calculate % Disease reduction over UTC as per the formula given above.

4) 1000 grain weight: Randomly take 10 samples from harvested grains of each plot at the time of harvest. Each samples with counted number of 1000 grains should be weighed and take average weight.

5) Effective Tillers: Count the number of tillers bearing panicle at the growth stage of grain filling from 1 sq.mt spot. Take such 5 spot per plot.
6) Plant height: Record the plant height by measuring (cm) from soil surface to the tip of the tallest panicle (awns excluded) at milk stage.

7) Plant Lodging: Record % of plant lodged from the entire plot by visual observation at the growth stage of mature grain.

8) Yield: Record the grain weight in gram from 5 sq. m spot per plot and convert it to quintal per acre at the time of harvest.

Table 1 Diseases control in Rice

Treatments (g.a.i./h) % Diseases reduction over UTC in Paddy
Sheath blight disease False smut disease Grain discoloration
MC+Tebu+Trifloxy,35+122.5+87.5 gai/h 94.26 96.12 98.86
MC+Tebu+Trifloxy,50+175+125 gai/h 96.74 98.46 100.00
MC+Tebu+Trifloxy,62.5+218.75+156.25 gai/h 98.63 98.86 100.00
MC+Tebu.62.5+218.75 gai/h 55.94 72.42 80.61
MC+Trifloxy, 62.5+156.25 gai/h 62.27 79.87 76.36
Tebu+Trifloxy,218.75+156.25 gai/h 72.84 85.46 88.68
MC, 62.5 gai/h 4.62 1.26 4.82
Tebu, 218.75 gai/h 52.36 68.18 71.46
Trifloxy, 156.25 gai/h 64.72 74.26 72.63
Untreated Check (UTC) 0.00 0.00 0.00

Where MC- Mepiquat Chloride, Tebu- Tebuconazole, Trifloxy-Trifloxystrobin, g.a.i. /h – gram active ingredient per hectare.

Sheath blight caused by Rhizoctonia solani, False smut by Ustilaginoidea virens, Grains discoloration by Curvularia spp., Helminthosporium spp., Drechslera spp.

The results of above experiment shows that inventive mixture of Mepiquat Chloride + Tebuconazole + Trifloxystrobin shows superior control of rice diseases like sheath blight, false smut and grain discoloration, compared to their two way mixtures as well as their individual application.

Table 2 Effective tillers
Treatments (g.a.i./h) No. of Effective Tillers/hill % Increase over Untreated
MC+Tebu+Trifloxy,35+122.5+87.5 gai/h 41.94 25.04
MC+Tebu+Trifloxy,50+175+125 gai/h 43.86 30.77
MC+Tebu+Trifloxy,62.5+218.75+156.25 gai/h 43.10 28.50
MC+Tebu.62.5+218.75 gai/h 37.72 12.46
MC+Trifloxy, 62.5+156.25 gai/h 36.92 10.08
Tebu+Trifloxy,218.75+156.25 gai/h 36.64 9.24
MC, 62.5 gai/h 36.24 8.05
Tebu, 218.75 gai/h 35.58 6.08
Trifloxy, 156.25 gai/h 35.08 4.59
Untreated Check (UTC) 33.54 0.00

Where MC- Mepiquat Chloride, Tebu- Tebuconazole, Trifloxy-Trifloxystrobin, g.a.i. /h – gram active ingredient per hectare.

The inventive combination of Mepiquat chloride+Tebuconazole+Trifloxystrobin produces maximum number of effective tillers compared to other tested combinations as well as solo application.

Table 3 Plant height and lodging at harvest
Treatments (g.a.i./h) Plant height (cm) % Lodging at time of harvest
MC+Tebu+Trifloxy,35+122.5+87.5 gai/h 97.6 0.0
MC+Tebu+Trifloxy,50+175+125 gai/h 93.4 0.0
MC+Tebu+Trifloxy,62.5+218.75+156.25 gai/h 93.2 0.0
MC+Tebu.62.5+218.75 gai/h 102.8 15.6
MC+Trifloxy, 62.5+156.25 gai/h 108.8 25.8
Tebu+Trifloxy,218.75+156.25 gai/h 114.2 45.2
MC, 62.5 gai/h 108.6 32.4
Tebu, 218.75 gai/h 113.8 46.6
Trifloxy, 156.25 gai/h 115.4 50.4
Untreated Check (UTC) 115.2 64.8

Where MC- Mepiquat Chloride, Tebu- Tebuconazole, Trifloxy-Trifloxystrobin, g.a.i. /h – gram active ingredient per hectare.

Plant height has been reduced by two sequential applications (at 20 and 50 days after transplanting) of inventive combination of Mepiquat chloride+Tebuconazole+Trifloxystrobin which ultimately contributes to the reduction in plant lodging and thereby grain quality and yield.
Table 4: Grain yield (quintal/acre)

Treatments (g.a.i./h) Quintal/acre % Increase over Untreated
MC+Tebu+Trifloxy,35+122.5+87.5 gai/h 22.10 74.57
MC+Tebu+Trifloxy,50+175+125 gai/h 22.48 77.57
MC+Tebu+Trifloxy,62.5+218.75+156.25 gai/h 22.64 78.83
MC+Tebu.62.5+218.75 gai/h 19.18 51.50
MC+Trifloxy, 62.5+156.25 gai/h 18.96 49.76
Tebu+Trifloxy,218.75+156.25 gai/h 19.66 55.29
MC, 62.5 gai/h 13.78 8.85
Tebu, 218.75 gai/h 17.22 36.02
Trifloxy, 156.25 gai/h 17.58 38.86
Untreated Check (UTC) 12.66 0.00

Where MC- Mepiquat Chloride, Tebu- Tebuconazole, Trifloxy-Trifloxystrobin, g.a.i. /h – gram active ingredient per hectare.

The yield data shows that inventive combination of Mepiquat chloride+ Tebuconazole+ Trifloxystrobin produces higher grain yield, which is approximately > 74% compared to untreated and >19% compared to Tebuconazole+Trifloxystrobin.

Example 2 Testing on wheat crop

The synergistic mixture of Mepiquat chloride, Tebuconazole and Trifloxystrobin was also evaluated on wheat crop.

Details of Experiment:
a) Experiment design: Randomized Block Design
b) Replication : Three
c) Treatments : Eight
d) Plot size : 50 sq. m.
e) Seed rate : 40 kg per acre
f) Test Crop & Variety : Wheat, HD 2967
g) Time of application : Two spray. 1st at 30 DAS and 2nd at 60 DAS (Days After Sowing)
h) Spray method : Manually operated knap sack sprayer, using 500 liter per hectare water Volume
Evaluation Method:
1) Yellow stripe rust: Observed 25 plants per plot and observe 4 leaves) from lower leaf to flag leaf, -3, -2, -1 and flag leaf). Record disease incidence and severity on each leaf. 4 leaf per plant and 25 plants per plot. Record disease severity by adopting 0~10 score as below.
Score Description
0 No Infection
1 up to 10% leaf area infected
3 21-30% leaf area infected and covered with rust symptoms
5 41-50% leaf area infected and covered with rust symptoms
7 61-70% leaf area infected and covered with rust symptoms
9 >90% leaf area infected and covered with rust symptoms

Calculate disease severity as
PDI (%) = Sum of numerical rating x 100 .
Total no. of plants observed x Max. Grade
The percent disease reduction over Untreated Check (UTC) was calculated as below:
% Reduction over UTC = 100 - (100 x PDI % in Treatment )
(PDI % in Untreated Check)
2) 1000 grain weight: Randomly take 10 samples from harvested grains of each plot at the time of harvest. Each samples with counted number of 1000 grains should be weighed and take average weight.
3) Effective Tillers: Count the number of tillers bearing panicle at the growth stage of grain filling from 1 sq.mt spot. Take such 5 spot per plot.
4) Plant height: Record the plant height by measuring (cm) from soil surface to the tip of the tallest panicle (awns excluded) at milk stage.
5) Plant Lodging: Record % of plant lodged from the entire plot by visual observation at the growth stage of mature grain.
6) Yield: Record the grain weight in gram from 5 sq. m spot per plot and convert it to quintal per acre at the time of harvest.
Table 5: Yellow rust, Puccinia striiformis control in Wheat crop
Treatments (g.a.i./h) % Yellow rust control
Obs. Value Exp. Value Ratio O/E
MC+Tebu+Trifloxy,35+122.5+87.5 gai/h 99.24 76.27 1.30
MC+Tebu,35+122.5 gai/h 44.28 46.42 0.95
MC+Trifloxy,35+87.5 gai/h 56.86 57.77 0.98
Tebu 122.5 +Trifloxy 87.5 gai/h 82.2 75.12 1.09
MC, 35 gai/h 4.62
Tebu, 122.5 gai/h 43.82
Trifloxy, 87.5 gai/h 55.72
Untreated Check (UTC) 0.00

Where MC- Mepiquat Chloride, Tebu- Tebuconazole, Trifloxy-Trifloxystrobin, g.a.i. /h – gram active ingredient per hectare.
The inventive combination of Mepiquat chloride+Tebuconazole+Trifloxystrobin shows synergism in terms of an excellent control of yellow rust disease compared to the other tested combination and solo active ingredient.

Table 6: Effective tillers
Treatments (g.a.i./h) No. of Effective Tillers % Increase over Untreated
MC+Tebu+Trifloxy,35+122.5+87.5 gai/h 165.4 17.81
MC+Tebu,35+122.5 gai/h 151.6 7.98
MC+Trifloxy,35+87.5 gai/h 152.6 8.69
Tebu 122.5 +Trifloxy 87.5 gai/h 146.2 4.13
MC, 35 gai/h 145.8 3.85
Tebu, 122.5 gai/h 143.6 2.28
Trifloxy, 87.5 gai/h 144.4 2.85
Untreated Check (UTC) 140.4 0.00

Where MC- Mepiquat Chloride, Tebu- Tebuconazole, Trifloxy-Trifloxystrobin, g.a.i. /h – gram active ingredient per hectare.

The number of effective has been found increased by >17% in the treatment of Mepiquat chloride+Tebuconazole+Trifloxystrobin, which directly contributes to the yield.

Table 7: Plant height and lodging

Treatments (g.a.i./h) Plant height (cm) % Lodging at time of harvest
MC+Tebu+Trifloxy,35+122.5+87.5 gai/h 95.6 0.0
MC+Tebu,35+122.5 gai/h 94.4 15.6
MC+Trifloxy,35+87.5 gai/h 94.2 22.8
Tebu 122.5 +Trifloxy 87.5 gai/h 101.2 30.7
MC, 35 gai/h 97.6 20.4
Tebu, 122.5 gai/h 100.4 30.7
Trifloxy, 87.5 gai/h 102.6 50.5
Untreated Check (UTC) 101.8 68.9

Where MC- Mepiquat Chloride, Tebu- Tebuconazole, Trifloxy-Trifloxystrobin, g.a.i. /h – gram active ingredient per hectare.

Table 8: 1000 grain weight

Treatments (g.a.i./h) 1000 grain weight (g) % Increase over Untreated
MC+Tebu+Trifloxy,35+122.5+87.5 gai/h 51.26 26.44
MC+Tebu,35+122.5 gai/h 46.74 15.29
MC+Trifloxy,35+87.5 gai/h 47.86 18.06
Tebu 122.5 +Trifloxy 87.5 gai/h 48.64 19.98
MC, 35 gai/h 43.24 6.66
Tebu, 122.5 gai/h 44.68 10.21
Trifloxy, 87.5 gai/h 45.82 13.02
Untreated Check (UTC) 40.54 0.00

Where MC- Mepiquat Chloride, Tebu- Tebuconazole, Trifloxy-Trifloxystrobin, g.a.i. /h – gram active ingredient per hectare.

Table 9: Yield (quintal/acre)

Treatments (g.a.i./h) Quintal / ha. % Increase over Untreated
MC+Tebu+Trifloxy,35+122.5+87.5 gai/h 27.54 57.96
MC+Tebu,35+122.5 gai/h 24.86 42.63
MC+Trifloxy,35+87.5 gai/h 24.69 41.62
Tebu 122.5 +Trifloxy 87.5 gai/h 24.93 43.00
MC, 35 gai/h 21.55 23.63
Tebu, 122.5 gai/h 22.10 26.80
Trifloxy, 87.5 gai/h 23.09 32.45
Untreated Check (UTC) 17.43 0.00

Where MC- Mepiquat Chloride, Tebu- Tebuconazole, Trifloxy-Trifloxystrobin, g.a.i. /h – gram active ingredient per hectare.

The experiment results on plant height, plant lodging, 1000 grain weight and yield at harvest shows that the synergistic mixture of Mepiquat chloride+Tebuconazole+Trifloxystrobin effectively reduce plant height and there by reduction in lodging, which directly contributes to the higher grain yield and grain quality in comparison to the their solo application as well as their two way combinations, like Mepiquat chloride +Tebuconazole, Mepiquat chloride + Trifloxystrobin and Tebuconazole + Trifloxystrobin.

Example 3: Seed treatment in Groundnut Arachis hypogaea

The synergistic mixture of Mepiquat chloride, Tebuconazole and Trifloxystrobin was also evaluated as a seed treatment in groundnut crop.

Details of Experiment:
a) Experiment design : Randomized Block Design
b) Replication : Four
c) Treatments : Eight
d) Plot size : 50 sq. m.
e) Seed rate : 40 kg per acre
f) Test Crop & Variety : Groundnut, GG-2
g) Method of application : Seed treatment at time of sowing
The seed of groundnut variety GG-2 were treated with the respective treatment and 100 number of treated seeds were sown in micro plot. Each micro plot represents one replication, such four replications per treatment were taken. The micro plot soil was sick with Rhizoctonia solani, Aspergillus spp., Phytophthora spp. and Sclerotium rolfsii. Regular watering were done. The observations on seed germination were recorded at 10 days after sowing. The data represented in the below table.

Table 10: Seed treatment in Groundnut

Treatment details % Seed germination
Obs.Value Cal.Value Ratio
MC+Tebu+Trifloxy,0.25+0.2+0.05 gai/10 kg seed 95.8 88.84 1.08
MC+Tebu,0.25+0.2 gai/10 kg seed 72.2 76.95 0.94
MC+Trifloxy,0.25+0.05 gai/10 kg seed 70.6 71.54 0.99
Tebu+Trifloxy,0.2+0.05 gai/10 kg seed 82.2 81.03 1.01
MC, 0.25 gai/10 kg seed 41.2
Tebu, 0.2 gai/10 kg seed 60.8
Trifloxy, 0.05 gai/10 kg seed 51.6
Untreated Check (UTC) 38.6

Where MC- Mepiquat Chloride, Tebu- Tebuconazole, Trifloxy-Trifloxystrobin, g.a.i.- gram active ingredient.

The results of above experiment shows that Mepiquat chloride + Tebuconazole + Trifloxystrobin gave highest germination of groundnut seed and shows synergism, which proves that the synergistic mixture also provides better protection to groundnut seeds and seedlings from soil born fungal diseases.
Example 4:

The synergistic mixture of (i) Mepiquat chloride + Prothioconazole + Trifloxystrobin and (ii) Mepiquat chloride + Prothioconazole + Picoxystrobin weres also evaluated in chilli (Capsicum annuum) crop.

Details of Experiment:
a) Experiment design : Randomized Block Design
b) Replication : Three
c) Treatments : Twelve
d) Plot size : 30 sq. m.
e) Spacing : 100 cm x 50 cm
f) Test Crop & Variety : Chilli, Gayathri (Green fruit purpose)
g) Time of application : Two spray, 1st at 60 days, 2nd at 75 days after transplanting
h) Spray Volume : 1st spray- 500 l/h and 2nd spray-550 l/h
i) Method of application : Foliar spray with knap sack sprayer
The chilli nursery was raised and transplanted in the main field at row to row 100 cm and plant to plant 50 cm spacing. Rest of the agronomic practices like fertilizer applications, weeding, irrigation (drip irrigation) were as per the standard agronomic practices. The spraying was done at 60 and 75 days after transplanting with the help of knapsack sprayer. The observations on severity and incidence of various diseases and fruit counts were taken as follow:

1) Die back disease :

Observations recorded at 15 days after 2nd application by observing 25 plant per plot. The disease severity recorded by following 0-9 scale described as below;
Grade Percent branches & foliage infected per plant
0 No infection to branches and leaves
1 1-10% Branches or leaves infected
3 11-25% Branches or leaves infected
5 26-50% Branches or leaves infected
7 51-75% Branches or leaves infected
9 >75% Branches or leaves infected.

% Disease Incidence = No. of plants infected X 100.
No. of plants observed

Die back PDI = _________Sum of numerical disease rating X 100
Total no. of samples X Maximum of disease rating scale

2) Fruit rot disease:
The fruit rot disease severity and incidence recorded in similar way as die-back. Record the number of infected fruits and healthy furits per plant to calculate disease incidence and rate the infected fruits in 0 to 9 Grade for severity and calculate % fruit rot incidence and % fruit rot severity.

3) Green fruit :
Count the no. of green fruits per plant at 15 days after 2nd spray. Take the counting from 25 plants per plot.

Table 11: Fungal disease control in Chilli
Treatment details Die back, Colletotrichum capsici Fruit rot, Phytophthora capsici
Severity (%) Incidence (%) Severity (%) Incidence (%)
MC+Prothioconazole+Trifloxystrobin, 40+120+90 gai/h 0.00 0 0.00 0
MC+Prothioconazole+Picoxystrobin, 40+120+90 gai/h 0.00 0 0.00 0
MC+Prothioconazole, 40+120 gai/h 34.28 17.26 19.27 13.28
MC+Trifloxystrobin, 40+90 gai/h 18.48 9.72 16.84 11.95
MC+Picoxystrobin, 40+90 gai/h 13.72 7.48 13.73 9.38
Prothioconazole+Trifloxystrobin, 120+ 90 gai/h 9.38 5.38 8.38 5.84
Prothioconazole+Picoxystrobin, 120+90 gai/h 7.58 3.28 5.83 3.73
MC 40 gai/h 56.36 25.64 58.28 26.64
Prothioconazole 120 gai/h 40.18 20.84 40.28 19.83
Trifloxystrobin 90 gai/h 28.62 14.65 26.84 16.64
Picoxystrobin 90 gai/h 20.74 12.83 22.84 15.26
Untreated Check (UTC) 61.38 28.84 65.83 30.84

MC –Mepiquat Chloride, gai/h- Gram active ingredient per hectare

Table 12: Green fruits per plant
Treatment details No. of green fruits per plant
Obs.Value Cal.Value Ratio
MC+Prothioconazole+Trifloxystrobin, 40+120+90 gai/h 128.4 95.38 1.35
MC+Prothioconazole+Picoxystrobin, 40+120+90 gai/h 132.6 95.81 1.38
MC+Prothioconazole, 40+120 gai/h 81.8 83.62 0.98
MC+Trifloxystrobin, 40+90 gai/h 85.2 87.65 0.97
MC+Picoxystrobin, 40+90 gai/h 86.4 88.79 0.97
Prothioconazole+Trifloxystrobin, 120+ 90 gai/h 87.6 89.45 0.98
Prothioconazole+Picoxystrobin, 120+90 gai/h 87.2 90.43 0.96
MC 40 gai/h 56.2
Prothioconazole 120 gai/h 62.6
Trifloxystrobin 90 gai/h 71.8
Picoxystrobin 90 gai/h 74.4
Untreated Check (UTC) 42.8

MC –Mepiquat Chloride, gai/h- Gram active ingredient per hectare

The inventive mixture of Mepiquat chloride+Prothioconazole+Trifloxystrobin and Mepiquat Chloride+Prothioconazole+Picoxystrobin provides excellent control of fungal diseases like die back caused by Colletotrichum capsici and fruit rot caused by Phytophthora capsici. Both inventive mixtures tested also shows synergism in terms of number of green fruit yield per plant which contributes to the higher fruit yield.

Example 5:
The synergistic mixture of (i) Mepiquat chloride+Epoxiconazole+Azoxystrobin, (ii) Mepiquat chloride+Epoxiconazole+Picoxystrobin, (iii) Mepiquat chloride+Epoxiconazole+Pyraclostrobin and (iv) Mepiquat chloride+Epoxiconazole+Trifloxystrobin were evaluated in Tomato (Solanum lycopericum) crop.

Details of Experiment:
a) Experiment design : Randomized Block Design
b) Replication : Three
c) Treatments : Twenty
d) Plot size : 25 sq. m.
e) Spacing : 90 cm x 45 cm
f) Test Crop & Variety : Tomato, Himsikhar
g) Time of application : Two spray, 1st at 60 days, 2nd at 80 days after transplanting
h) Spray Volume : 1st spray- 500 l/h and 2nd spray-600 l/h
i) Method of application : Foliar spray with knap sack sprayer
The tomato nursery was raised and transplanted in the main field at row to row 90 cm and plant to plant 45 cm spacing. Rest of the agronomic practices like fertilizer applications, weeding, irrigation (drip irrigation) were as per the standard agronomic practices. The spraying was done at 60 and 80 days after transplanting with the help of knapsack sprayer. The observations on severity and incidence of various diseases and fruit counts were taken as follow:

1). Early Blight:
The observations on Early blight (caused by Alternaria solani): Disease severity was assessed by using following disease rating scale.

Score Symptoms
0 No symptom of disease on plant
1 Small, irregular brown spots covering 1% or less the leaf area
3 Small, irregular brown spots with concentric rings, covering 1-10% leaf area
5 Lesion enlarging, irregular brown spots with concentric rings, covering 11-25% leaf area
7 Lesions coalesce to form irregular dark brown patches with concentric rings, covering 26-50% of leaf area, Lesions on stem and petioles
9 Lesions coalesce to form irregular dark brown patches with concentric rings, covering more than 51% of leaf area, Lesions on stem and petioles

Sum of all disease rating x 100
Percent Disease Intensity (PDI) = ------------------------------------------------------
Total number of leaves assessed x Maximum rating

% Disease Control = 100 – (100 – PDI in treatment / PDI in Untreated plot)

Table 13: Early blight control in Tomato
Treatment details Early Blight, Alternaria solani
Incidence (%) PDI / Severity (%)
MC+Epoxiconazole+Azoxystrobin,48+60+96 gai/h 0.00 0.00
MC+Epoxiconazole+Picoxystrobin,48+60+96 gai/h 0.00 0.00
MC+Epoxiconazole+Pyraclostrobin,48+60+96 gai/h 0.00 0.00
MC+Epoxiconazole+Trifloxystrobin,48+60+96 gai/h 0.00 0.00
MC+Epoxiconazole,48+60 gai/h 14.28 14.72
MC+Azoxystrobin,48+96 gai/h 12.38 13.29
MC+Picoxystrobin,48+96 gai/h 14.83 15.38
MC+Pyraclostrobin,48+96 gai/h 12.37 14.28
MC+Trifloxystrobin,48+96 gai/h 11.82 12.67
Epoxiconazole+Azoxystrobin,60+96 gai/h 8.72 14.28
Epoxiconazole+Picoxystrobin,60+96 gai/h 7.92 13.28
Epoxiconazole+Pyraclostrobin,60+96 gai/h 10.27 11.94
Epoxiconazole+Trifloxystrobin,60+96 gai/h 8.42 13.28
MC,48 gai/h 30.28 56.73
Epoxiconazole, 60 gai/h 22.75 46.42
Azoxystrobin, 96 gai/h 16.28 26.72
Picoxystrobin, 96 gai/h 12.74 24.73
Pyraclostrobin, 96 gai/h 13.93 25.38
Trifloxystrobin, 96 gai/h 16.38 26.27
Untreated Check (UTC) 37.28 64.28

Table 14: Fruit setting in Tomato
Treatment details No. of fruits per plant
Obs.Value Cal.Value Ratio
MC+Epoxiconazole+Azoxystrobin,48+60+96 gai/h 38.27 34.57 1.11
MC+Epoxiconazole+Picoxystrobin,48+60+96 gai/h 40.18 32.73 1.23
MC+Epoxiconazole+Pyraclostrobin,48+60+96 gai/h 41.63 35.39 1.18
MC+Epoxiconazole+Trifloxystrobin,48+60+96 gai/h 39.64 33.34 1.19
MC+Epoxiconazole,48+60 gai/h 14.72 16.29 0.90
MC+Azoxystrobin,48+96 gai/h 25.83 27.88 0.93
MC+Picoxystrobin,48+96 gai/h 23.61 25.85 0.91
MC+Pyraclostrobin,48+96 gai/h 27.84 28.79 0.97
MC+Trifloxystrobin,48+96 gai/h 25.73 26.53 0.97
Epoxiconazole+Azoxystrobin,60+96 gai/h 30.12 29.09 1.04
Epoxiconazole+Picoxystrobin,60+96 gai/h 28.24 27.10 1.04
Epoxiconazole+Pyraclostrobin,60+96 gai/h 30.18 29.98 1.01
Epoxiconazole+Trifloxystrobin,60+96 gai/h 28.12 27.76 1.01
MC,48 gai/h 7.73
Epoxiconazole, 60 gai/h 9.28
Azoxystrobin, 96 gai/h 21.84
Picoxystrobin, 96 gai/h 19.64
Pyraclostrobin, 96 gai/h 22.82
Trifloxystrobin, 96 gai/h 20.37
Untreated Check (UTC) 6.82

MC- Mepiquat Chloride

The efficacy data of the following inventive mixtures of the present invention.
Mepiquat chloride + Epoxiconazole + Azoxystrobin,
Mepiquat chloride + Epoxiconazole + Picoxystrobin,
Mepiquat chloride + Epoxiconazole + Pyraclostrobin and
Mepiquat chloride + Epoxiconazole + Trifloxystrobin on Tomato crop shows an excellent control of early blight diseases as well as higher number of tomato fruits per plant.

STABILITY DATA

FORMULATION RECIPE

1) Mepiquat Chloride 10%+Tebuconazole 35%+Trifloxystrobin 25% WG (Water Dispersible Granules)

Compositions Percent (w/w)
Mepiquat Chloride (90% purity ) 11.33
Tebuconazole (96 % purity) 36.66
Trifloxystrobin (95% purity) 26.52
Alkylated naphthalene sulfonate, sodium salt 6.00
Sodium Polycarboxylate 3.00
Sodium Lauryl Sulfate 3.00
Polyacrylate polymer sodium salt 2.50
Silicone antifoam 0.50
Corn starch 6.99
China Clay 3.50
TOTAL 100.00

Table 15
Storage stability Study-Mepiquat Chloride 10%+Tebuconazole 35%+Trifloxystrobin 25% WG (Water Dispersible Granules)
Parameters Specification Initial Heat stability at 54+2 0C for 14 days Cold storage stability at 0+20C for 14 days
Description Off white colour granules Complies Complies Complies
Mepiquat Chloride content 9.5-10.5 10.3 10.25 10.3
Tebuconazole content 33.25-36.75 35.39 35.35 35.39
Trifloxystrobin content 23.75-26.25 25.34 25.29 25.34
Mepiquat Chloride suspensibility Minimum 70% 98.41 98.04 98.37
Tebuconazole suspensibility Minimum 70% 85.58 85.19 85.41
Trifloxystrobin suspensibility Minimum 70% 89.87 86.61 89.74
pH 5 to 7 6.62 6.58 6.6
Wettability Maximum 60 s 7 7 8
Wet Sieve(45 micron) Minimum 98.5% 99.69 99.53 99.58
Bulk Density 0.45-0.75 0.55 0.55 0.55
Moisture Content Maximum 2.0% 1 0.8 1
Room temperature storage data
Parameters Specification Study Duration
1 month 6 months 12 months 24 months
Description Off white colour granuls Complies Complies Complies Complies
Mepiquat Chloride content 9.5-10.5 10.12 10.11 10.07 10.01
Tebuconazole content 33.25-36.75 35.31 35.28 35.24 35.07
Trifloxystrobin content 23.75-26.25 25.33 25.27 25.21 25.12
Mepiquat Chloride suspensibility Minimum 70% 98.04 97.83 97.20 96.52
Tebuconazole suspensibility Minimum 70% 85.41 85.07 85.66 85.04
Trifloxystrobin suspensibility Minimum 70% 89.79 89.71 89.21 88.72
pH 5 to 7 6.62 6.62 6.6 6.58
Wettability Maximum 60 s 7 7 8 7
Wet Sieve(45 micron) Minimum 98.5% 99.6 99.5 99.5 99.4
Bulk Density 0.45-0.75 0.55 0.55 0.55 0.55
Moisture Content Maximum 2.0% 0.8 0.7 0.6 0.4

2) Mepiquat Chloride 5%+Tebuconazole 17.5%+Trifloxystrobin 12.5% SC (Suspension Concentrate)

Composition Percent (w/w)
Mepiquat Chloride (90% purity ) 5.77
Tebuconazole (96 % purity) 18.43
Trifloxystrobin (95% purity) 13.36
Nonionic Surfactant containing alcohol alkoxylates 2.00
Acrylic graft copolymer 3.00
Ethoxylated polyarylphenol 0.50
Alkylated naphthalene sulfonate, sodium salt 0.50
Silicone antifoam 1.00
Benzisothiazoline 0.20
Glycol 5.00
Polysacharide 0.20
Water 50.04
TOTAL 100.00

Table 16
Storage stability Study-Mepiquat Chloride 5% + Tebuconazole 17.5%+Trifloxystrobin 12.5% SC (Suspension Concentrate)
Parameters Specification
(In House) Initial Heat stability at 54+2 0C for 14 days Cold storage stability at 0+2 0C for 14 days
Description Off White colour flowable liquid Complies Complies Complies
Mepiquat Chloride content 4.75-5.25 5.21 5.01 5.21
Tebuconazole content 16.625-18.375 17.98 17.82 17.97
Trifloxystrobin content 11.875-13.125 12.77 12.59 12.77
Mepiquat Chloride suspensibility Minimum 80% 98.75 97.3 98.71
Tebuconazole suspensibility Minimum 80% 90.04 89.68 89.04
Trifloxystrobin suspensibility Minimum 80% 95.77 95.52 95.75
pH 5 to 7 6.5 6.5 6.5
Particle size D50 <3, D90 <10 2.5, 8 2.5, 8.2 2.9, 8.9
Pourability 95 % min 97 96 97
Specific gravity 1.05 – 1.15 1.1 1.1 1.1
Viscosity 350 -800 cps 500 510 530
Room temperature storage data
Specification Study Duration
Parameters In House 1 month 6 months 12 months 24 months
Description Off White colour flowable liquid Complies Complies Complies Complies
Mepiquat Chloride content 4.75-5.25 5.17 5.16 5.13 5.09
Tebuconazole content 16.625-18.375 17.96 17.93 17.85 17.72
Trifloxystrobin content 11.875-13.125 12.77 12.7 17.68 17.61
Mepiquat Chloride suspensibility Minimum 80% 98.75 98.01 97.9 97.5
Tebuconazole suspensibility Minimum 80% 90.01 89.95 89.88 89.21
Trifloxystrobin suspensibility Minimum 80% 95.75 95.7 95.55 95.05
pH 5 to 7 6.5 6.48 6.46 6.41
Particle size D50 <3,
D90 <10 2.5, 8 2.5, 8 2.5, 8 2.5, 8
Pourability 95 % min 97 97 97 97
Specific gravity 1.05 – 1.15 1.15 1.15 1.16 1.16
Viscosity 350 -800 cps 500 521 532 544

3) Mepiquat Chloride 16%+Epoxiconazole 20%+Trifloxystrobin 32% WG (Water Dispersible Granules)
Compositions Percent (w/w)
Mepiquat Chloride (90% purity ) 18.00
Epoxiconazole (95% purity) 21.26
Trifloxystrobin (95% purity) 33.89
Alkylated naphthalene sulfonate, sodium salt 5.00
Sodium dioctyl sulfosuccinate 1.00
Sodium Polycarboxylate 3.00
PPT Silica 1.00
Sodium Lauryl Sulfate 4.00
Silicone antifoam 0.50
Corn Starch 7.00
China Clay 5.35
TOTAL 100.00

Table 17
Storage stability Study-Mepiquat Chloride 16%+Epoxiconazole 20%+Trifloxystrobin 32% WG (Water Dispersible Granules)
Parameters Specification Initial Heat stability at 54+2 0C for 14 days Cold storage stability at 0+2 0C for 14 days
Description Beige coloured granules Complies Complies Complies
Mepiquat Chloride content 15.2-16.8 16.26 16.14 16.26
Epoxiconazole content 19.0-21.0 20.34 20.29 20.34
Trifloxystrobin content 30.4-33.6 32.53 32.37 32.53
Mepiquat Chloride suspensibility Minimum 70% 97.75 97.70 97.75
Epoxiconazole suspensibility Minimum 70% 92.55 92.39 92.55
Trifloxystrobin suspensibility Minimum 70% 91.60 91.48 91.6
pH 5 to 7 6.9 6.9 6.9
Wettability Maximum 60 s 7 8 7
Wet Sieve (45 micron) Minimum 98.5% 99.6 99.4 99.6
Bulk Density 0.45-0.75 0.7 0.7 0.7
Moisture Content Maximum 2.0% 1 0.7 1
Room temperature storage data
Parameters Specification Study Duration
1 month 6 months 12 months 24 months
Description Beige coloured granules Complies Complies Complies Complies
Mepiquat Chloride content 15.2-16.8 16.24 16.23 16.21 16.12
Epoxiconazole content 19.0-21.0 20.3 20.28 20.21 20.14
Trifloxystrobin content 30.4-33.6 32.5 32.49 32.42 32.29
Mepiquat Chloride suspensibility Minimum 70% 97.7 97.57 97.44 96.88
Epoxiconazole suspensibility Minimum 70% 92.5 92.42 92.12 91.78
Trifloxystrobin suspensibility Minimum 70% 91.6 91.51 91.15 90.45
pH 5 to 7 6.89 6.89 6.85 6.81
Wettability Maximum 60 s 7 7 8 9
Wet Sieve(45 micron) Minimum 98.5% 99.6 99.6 99.5 99.4
Bulk Density 0.45-0.75 0.5 0.5 0.5 0.5
Moisture Content Maximum 2.0% 1.4 1.3 1.2 1

4) Mepiquat Chloride 16%+Epoxiconazole 20%+Pyraclostrobin 32% WG (Water Dispersible Granules)

Compositions Percent (w/w)
Mepiquat Chloride ( 90% purity ) 18.00
Epoxiconazole ( 95 % purity) 21.26
Pyraclostrobin ( 95 % purity) 33.89
Alkylated naphthalene sulfonate, sodium salt 5.00
Polyacrylate polymer 2.00
Sodium Polycarboxylate 3.00
Sodium Lauryl Sulfate 4.00
Silicone antifoam 0.50
Corn starch 8.00
China Clay 4.35
TOTAL 100.00

Table 18
Storage stability Study-Mepiquat Chloride 16%+Epoxiconazole 20%+Pyraclostrobin 32% WG (Water Dispersible Granules)
Parameters Specification Initial Heat stability at 54+20C for 14 days Cold storage stability at 0+2 0C for 14 days
Description Beige coloured granules Complies Complies Complies
Mepiquat Chloride content 15.2-16.8 16.37 16.25 16.37
Epoxiconazole content 19.0-21.0 20.45 20.32 20.45
Pyraclostrobin content 30.4-33.6 32.6 32.48 32.6
Mepiquat Chloride suspensibility Minimum 70% 93.82 93.77 93.82
Epoxiconazole suspensibility Minimum 70% 90.27 90.15 90.27
Pyraclostrobin suspensibility Minimum 70% 90.55 90.25 90.55
pH 5 to 7 6.65 6.65 6.65
Wettability Maximum 60 s 7 8 7
Wet Sieve(45 micron) Minimum 98.5% 99.2 99.2 99.2
Bulk Density 0.45-0.75 0.6 0.6 0.6
Moisture Content Maximum 2.0% 1.1 0.6 1.1
Room temperature storage data
Parameters Specification Study Duration
1 month 6 months 12 months 24 months
Description Beige coloured granules Complies Complies Complies Complies
Mepiquat Chloride content 15.2-16.8 16.35 16.34 16.31 16.11
Epoxiconazole content 19.0-21.0 20.45 20.41 20.35 20.15
Pyraclostrobin content 30.4-33.6 32.5 32.46 32.37 32.01
Mepiquat Chloride suspensibility Minimum 70% 93.8 93.51 93.12 92.46
Epoxiconazole suspensibility Minimum 70% 90.1 89.98 89.66 88.92
Pyraclostrobin suspensibility Minimum 70% 90.45 90.21 90.13 89.54
pH 5 to 7 6.65 6.64 6.63 6.61
Wettability Maximum 60 s 6 7 7 8
Wet Sieve(45 micron) Minimum 98.5% 99.2 99.2 99.2 99.2
Bulk Density 0.45-0.75 0.6 0.6 0.6 0.6
Moisture Content Maximum 2.0% 1.1 1 0.8 0.7

5) Mepiquat Chloride 16%+Epoxiconazole 20%+Picoxystrobin 32% WG (Water Dispersible Granules)

Compositions Percent (w/w)
Mepiquat Chloride (90% purity ) 18.00
Epoxiconazole (95% purity) 21.26
Picoxystrobin (95% purity) 33.89
Alkylated naphthalene sulfonate, sodium salt 6.00
Sodium alkyl naphthalene sulfonate blend 2.00
Sodium Lauryl Sulfate 3.00
Silicone antifoam 0.50
Sodium Sulphate Anhydrous 2.00
Corn Starch 7.00
China Clay 6.35
TOTAL 100.00

Table 19
Storage stability Study-Mepiquat Chloride 16%+Epoxiconazole 20%+Picoxystrobin 32% WG (Water Dispersible Granules)
Parameters Specification Initial Heat stability at 54+2 0C for 14 days Cold storage stability at 0+2 0C for 14 days
Description Beige coloured granules Complies Complies Complies
Mepiquat Chloride content 15.2-16.8 16.38 16.29 16.38
Epoxiconazole content 19.0-21.0 20.55 20.42 20.55
Picoxystrobin content 30.4-33.6 32.65 32.58 32.65
Mepiquat Chloride suspensibility Minimum 70% 94.65 93.86 94.65
Epoxiconazole suspensibility Minimum 70% 92.82 92.54 92.82
Picoxystrobin suspensibility Minimum 70% 92.50 92.22 92.50
pH 5 to 7 6.5 6.45 6.5
Wettability Maximum 60 s 6 7 6
Wet Sieve(45 micron) Minimum 98.5% 99.6 99.2 99.6
Bulk Density 0.45-0.75 0.6 0.6 0.6
Moisture Content Maximum 2.0% 1.2 0.8 1.2
Room temperature storage data
Parameters Specification Study Duration
1 month 6 months 12 months 24 months
Description Beige coloured granules Complies Complies Complies Complies
Mepiquat Chloride content 15.2-16.8 16.35 16.32 16.23 16.14
Epoxiconazole content 19.0-21.0 20.51 20.5 20.49 20.35
Picoxystrobin content 30.4-33.6 32.64 32.61 32.55 32.16
Mepiquat Chloride suspensibility Minimum 70% 94.55 94.20 93.86 93.12
Epoxiconazole suspensibility Minimum 70% 92.81 92.48 92.02 91.82
Picoxystrobin suspensibility Minimum 70% 92.45 92.40 92.32 91.77
pH 5 to 7 6.5 6.49 6.45 6.41
Wettability Maximum 60 s 6 6 6 6
Wet Sieve(45 micron) Minimum 98.5% 99.6 99.5 99.3 99.1
Bulk Density 0.45-0.75 0.6 0.6 0.61 0.63
Moisture Content Maximum 2.0% 1 0.8 0.5 0.3

6) Mepiquat Chloride 10%+Tricyclazole 40%+Trifloxystrobin 20% WG (Water Dispersible Granules)

Compositions Percent (w/w)
Mepiquat Chloride (90% purity) 11.33
Tricyclazole (96% purity) 41.88
Trifloxystrobin (95 % purity) 21.26
Alkylated naphthalene sulfonate, sodium salt 6.00
Polyacrylate polymer sodium salt 2.00
Sodium Lauryl Sulfate 4.00
Silicone antifoam 0.50
Corn Starch 10.00
China Clay 3.03
TOTAL 100.00

Table 20
Storage stability Study-Mepiquat Chloride 10%+Tricyclazole 40%+Trifloxystrobin 20% WG (Water Dispersible Granules)
Parameters Specification Initial Heat stability at 54+2 0C for 14 days Cold storage stability at 0+2 0C for 14 days
Description Light orange colour granules Complies Complies Complies
Mepiquat Chloride content 9.5-10.5 10.35 10.32 10.35
Tricyclazole content 38.0-42.0 40.55 40.5 40.55
Trifloxystrobin content 19.0-21.0 20.5 20.48 20.5
Mepiquat Chloride suspensibility Minimum 70% 95.8 95.8 95.8
Tricyclazole suspensibility Minimum 70% 90.5 90.45 90.5
Trifloxystrobin suspensibility Minimum 70% 91.85 91.80 91.85
pH 5 to 7 6.9 6.9 6.9
Wettability Maximum 60 s 7 8 7
Wet Sieve(45 micron) Minimum 98.5% 99.7 99.7 99.7
Bulk Density 0.45-0.75 0.65 0.65 0.65
Moisture Content Maximum 2.0% 1 1 1
Room temperature storage data
Parameters Specification Study Duration
1 month 6 months 12 months 24 months
Description Light orange colour granules Complies Complies Complies Complies
Mepiquat Chloride content 9.5-10.5 10.35 10.35 10.3 10.22
Tricyclazole content 38.0-42.0 40.5 40.5 40.45 40.28
Trifloxystrobin content 19.0-21.0 20.5 20.5 20.48 20.22
Mepiquat Chloride suspensibility Minimum 70% 95.8 95.8 95.72 95.24
Tricyclazole suspensibility Minimum 70% 90.5 90.45 90.38 89.85
Trifloxystrobin suspensibility Minimum 70% 91.8 91.8 90.75 90.4
pH 5 to 7 6.89 6.89 6.85 6.81
Wettability Maximum 60 s 7 7 8 8
Wet Sieve(45 micron) Minimum 98.5% 99.6 99.6 99.6 99.6
Bulk Density 0.45-0.75 0.65 0.65 0.65 0.65
Moisture Content Maximum 2.0% 1 1 0.9 0.8

7) Mepiquat Chloride 10%+Tricyclazole 40%+Picoxystrobin 14% WG (Water Dispersible Granules)

Compositions Percent (w/w)
Mepiquat Chloride ( 90% purity) 11.33
Tricyclazole ( 96 % purity) 41.88
Picoxystrobin (95% purity) 14.95
Alkylated naphthalene sulfonate, sodium salt 6.00
Sodium alkyl naphthalene sulfonate blend 2.00
Sodium Polycarboxylate 2.00
Sodium Lauryl Sulfate 4.00
Silicone antifoam 0.50
Corn starch 9.00
Sodium SulfateAnhydrous 3.00
China Clay 5.34
TOTAL 100.00

Table 21
Storage stability Study-Mepiquat Chloride 10%+Tricyclazole 40%+Picoxystrobin 14% WG (Water Dispersible Granules)
Parameters Specification Initial Heat stability at 54+2 0C for 14 days Cold storage stability at 0+2 0C for 14 days
Description Light orange colour granules Complies Complies Complies
Mepiquat Chloride content 9.5-10.5 10.25 10.2 10.25
Tricyclazole content 38.0-42.0 40.38 40.25 40.38
Picoxystrobin content 13.3-14.7 14.35 14.3 14.35
Mepiquat Chloride suspensibility Minimum 70% 95.5 95.45 95.5
Tricyclazole suspensibility Minimum 70% 92.1 92.07 92.1
Picoxystrobin suspensibility Minimum 70% 91.25 91.19 91.25
pH 5 to 7 6.9 6.9 6.9
Wettability Maximum 60 s 7 8 7
Wet Sieve(45 micron) Minimum 98.5% 99.7 99.7 99.7
Bulk Density 0.45-0.75 0.65 0.65 0.65
Moisture Content Maximum 2.0% 1.5 1.5 1.5
Room temperature storage data
Parameters Specification Study Duration
1 month 6 months 12 months 24 months
Description Light orange colour granuals Complies Complies Complies Complies
Mepiquat Chloride content 9.5-10.5 10.25 10.24 10.22 10.16
Tricyclazole content 38.0-42.0 40.35 40.32 40.28 40.19
Picoxystrobin content 13.3-14.7 14.35 14.33 14.29 14.25
Mepiquat Chloride suspensibility Minimum 70% 95.5 95.5 95.05 94.88
Tricyclazole suspensibility Minimum 70% 92.1 92.1 91.95 91.75
Picoxystrobin suspensibility Minimum 70% 91.25 91.2 91.15 90.9
pH 5 to 7 6.89 6.89 6.85 6.81
Wettability Maximum 60 s 10 10 8 7
Wet Sieve(45 micron) Minimum 98.5% 99.6 99.6 99.6 99.6
Bulk Density 0.45-0.75 0.5 0.5 0.5 0.5
Moisture Content Maximum 2.0% 1.5 1.5 1.5 1.5

8) Mepiquat Chloride 5%+Tricyclazole 20.3%+Picoxystrobin 6.8% SC (Suspension Concentrate)

Composition Percent (w/w)
Mepiquat Chloride (90% purity) 5.77
Tricyclazole (96% purity) 21.35
Picoxystrobin (95% purity) 7.37
Ethoxylated Fatty Alcohol 1.50
Nonionic polyalkylene glycol ether 0.50
Acrylic graft copolymer 3.00
Alkylated naphthalene sulfonate, sodium salt 0.50
Ethylene oxide/propylene oxide block copolymer 0.50
Ethoxylated polyarylphenol 0.50
Silicone antifoam 1.00
Benzisothiazoline 0.20
Glycol 5.00
Polysaccharides 0.20
Water 52.61
TOTAL 100.00
Table 22

Storage stability Study-Mepiquat Chloride 5%+Tricyclazole 20.3%+Picoxystrobin 6.8% SC (Suspension Concentrate)
Parameters Specification (In House) Initial Heat stability at 54+2 0C for 14 days Cold storage stability at 0+2 0C for 14 days
Description Off white flowable liq. Complies Complies Complies
Mepiquat Chloride content 4.75-5.25 5.15 5.13 5.15
Tricyclazole content 19.29-21.32 20.42 20.4 20.42
Picoxystrobin content 6.46-7.14 6.95 6.92 6.95
Mepiquat Chloride suspensibility Minimum 80% 98.65 98.45 98.65
Tricyclazole suspensibility Minimum 80% 91.5 91.42 91.5
Picoxystrobin suspensibility Minimum 80% 95.7 95.62 95.7
pH 5 to 7 6.5 6.5 6.5
Particle size D50 <3, D90 <10 2.5, 8 2.5,
8 2.9,
8.9
Pourability 95 % min 97 96 97
Specific gravity 1.05 – 1.15 1.1 1.1 1.1
Viscosity 350 -800 cps 500 510 530
Room temperature storage data
Specification Study Duration
Parameters In House 1 month 6 months 12 months 24 months
Description Off white flowable liq. Complies Complies Complies Complies
Mepiquat Chloride content 4.75-5.25 5.14 5.14 5.13 5.12
Tricyclazole content 19.29-21.32 20.42 20.41 20.38 20.35
Picoxystrobin content 6.46-7.14 6.95 6.94 6.93 6.85
Mepiquat Chloride suspensibility Minimum 80% 98.6 98.6 98.5 98.3
Tricyclazole suspensibility Minimum 80% 91.5 91.5 91.5 91.32
Picoxystrobin suspensibility Minimum 80% 95.7 95.65 95.59 95.48
pH 5 to 7 6.5 6.5 6.5 6.5
Particle size D50 <3, D90 <10 2.5, 8 2.5, 8 2.5, 8 2.5, 8
Pourability 95 % min 97 97 97 97
Specific gravity 1.05 – 1.15 1.1 1.1 1.1 1.1
Viscosity 350 -800 cps 500 500 500 510

9) Mepiquat Chloride 10%+Prothioconazole 32%+Trifloxystrobin 24% WG (Water Dispersible Granules)

Compositions Percent (w/w)
Mepiquat Chloride (90% purity) 11.33
Prothioconazole (95% purity) 33.89
Trifloxystrobin (95% purity) 25.47
Alkylated naphthalene sulfonate, sodium salt 5.00
Polyacrylate polymer 2.00
Sodium Polycarboxylate 3.00
Sodium Lauryl Sulfate 4.00
Silicone antifoam 0.50
Corn Starch 8.00
China Clay 6.81
TOTAL 100.00

Table 23
Storage stability Study-Mepiquat Chloride 10%+Prothioconazole 32%+Trifloxystrobin 24% WG (Water Dispersible Granules)
Parameters Specification Initial Heat stability at 54+2 0C for 14 days Cold storage stability at 0+2 0C for 14 days
Description Beige coloured granules Complies Complies Complies
Mepiquat Chloride content 9.5-10.5 10.35 10.31 10.35
Prothioconazole content 30.4-33.6 32.2 32.2 32.2
Trifloxystrobin content 22.8-25.2 24.32 24.3 24.32
Mepiquat Chloride suspensibility Minimum 70% 98.15 98.05 98.15
Prothioconazole suspensibility Minimum 70% 92.18 92.15 92.15
Trifloxystrobin suspensibility Minimum 70% 92.55 92.48 92.55
pH 5 to 7 6.5 6.49 6.5
Wettability Maximum 60 s 7 8 7
Wet Sieve(45 micron) Minimum 98.5% 99.7 99.7 99.7
Bulk Density 0.45-0.75 0.55 0.55 0.55
Moisture Content Maximum 2.0% 1 0.7 0.9
Room temperature storage data
Parameters Specification Study Duration
1 month 6 months 12 months 24 months
Description Beige coloured granules Complies Complies Complies Complies
Mepiquat Chloride content 9.5-10.5 10.35 10.35 10.33 10.28
Prothioconazole content 30.4-33.6 32.2 32.19 32.15 32.08
Trifloxystrobin content 22.8-25.2 24.3 24.28 24.22 24.14
Mepiquat Chloride suspensibility Minimum 70% 98.10 98.09 98.07 98.01
Prothioconazole suspensibility Minimum 70% 92.15 92.07 92.05 92.01
Trifloxystrobin suspensibility Minimum 70% 92.48 92.41 92.39 92.31
pH 5 to 7 6.49 6.49 6.45 6.41
Wettability Maximum 60 s 7 8 8 8
Wet Sieve(45 micron) Mini 98.5% 99.7 99.7 99.7 99.7
Bulk Density 0.45-0.75 0.55 0.55 0.55 0.55
Moisture Content Maximum 2.0% 0.8 0.8 0.7 0.5

10) Mepiquat Chloride 5%+Prothioconazole 16%+Trifloxystrobin 12% SC (Suspension Concentrate)

Composition Percent (w/w)
Mepiquat Chloride (90% purity) 5.77
Prothioconazole (95% purity) 17.05
Trifloxystrobin (95% purity) 12.84
Nonionic surfactant Containing alcohol alkoxylates 2.00
Acrylic graft copolymer 3.00
Alkylated naphthalene sulfonate, sodium salt 0.50
Silicone antifoam 1.00
Benzisothiazoline 0.20
Glycol 5.00
Polysaccharides 0.20
Water 52.44
TOTAL 100.00

Table 24
Storage stability Study-Mepiquat Chloride 5%+Prothioconazole 16%+Trifloxystrobin 12% SC (Suspension Concentrate)
Parameters Specification
(In House) Initial Heat stability at 54+2 0C for 14 days Cold storage stability at 0+2 0C for 14 days
Description off white colour floable liquid Complies Complies Complies
Mepiquat Chloride content 4.75-5.25 5.12 5.08 5.11
Prothioconazole content 15.2-16.8 16.15 16.14 16.15
Trifloxystrobin content 11.4-12.6 12.25 12.22 12.22
Mepiquat Chloride suspensibility Minimum 80% 98.58 98.47 98.51
Prothioconazole suspensibility Minimum 80% 95.85 95.74 95.82
Trifloxystrobin suspensibility Minimum 80% 97.95 97.9 97.87
pH 5 to 7 6.5 6.5 6.5
Particle size D50 <3, D90 <10 2.5, 8 2.5, 8.4 2.9, 8.9
Pourability 95 % min 97 96 97
Specific gravity 1.05 – 1.15 1.125 1.131 1.127
Viscosity 350 -800 cps 500 510 530
Room temperature storage data
Specification Study Duration
Parameters In House 1 month 6 months 12 months 24 months
Description off white colour floable liquid Complies Complies Complies Complies
Mepiquat Chloride content 4.75-5.25 5.11 5.11 5.09 5.08
Prothioconazole content 15.2-16.8 16.14 16.13 16.11 16.08
Trifloxystrobin content 11.4-12.6 12.23 12.22 12.21 12.18
Mepiquat Chloride suspensibility Minimum 80% 98.5 98.48 98.41 98.35
Prothioconazole suspensibility Minimum 80% 95.8 95.78 95.75 95.62
Trifloxystrobin suspensibility Minimum 80% 97.88 97.86 97.79 97.71
pH 5 to 7 6.5 6.49 6.48 6.45
Particle size D50 <3, D90 <10 2.5, 7.9 2.5, 8.2 2.5, 8.3 2.5, 8.5
Pourability 95 % min 97 97 97 97
Specific gravity 1.05 – 1.15 1.135 1.137 1.129 1.131
Viscosity 350 -800 cps 500 500 505 510

,CLAIMS:1. A synergistic fungicidal composition comprising:

I) Mepiquat or a salt thereof in the range from 1 to 20 % by weight of the composition;
II) triazole compound in the range from 1 to 40% by weight of the composition wherein the said triazole compound is selected from the group consisiting of Difenoconazole, Epoxiconazole, Flusilazole, Hexaconazole, Propiconazole, Prothioconazole, Tebuconazole or Tricyclazole;
III) strobilurin compound in the range from 1 to 40 % by weight of the composition wherein the said strobilurin compound is selected from the group consisting of Azoxystrobin, Trifloxystrobin, Metominostrobin, Pyraclostrobin, Picoxystrobin, Orysastrobin, Coumoxystrobin and Kresoxim Methyl;
IV) one or more inactive excipient.

2. The synergistic fungicidal composition as claimed in claim 1, wherein the inactive excipients are selected from the group consisting of defoamer/anti-foaming agent, biocide/preservative, emulsifier, spreader, wetting agent, dispersant, co-dispersant, suspending agents, anti-freezing agent, , anti caking agent, rheology modifier/thickener, binder or filler .

3. The synergistic fungicidal composition as claimed in claim 1 or claim 2, wherein triazole compound is Tebuconazole and strobilurin compound is Trifloxystrobin.

4. The synergistic fungicidal composition as claimed in claim 1 or claim 2, wherein triazole compound is Epoxiconazole and strobilurin compound is Azoxystrobin.

5. The synergistic fungicidal composition as claimed in claim 1 or claim 2, wherein triazole compound is Epoxiconazole and strobilurin compound is Trifloxystrobin.

6. The synergistic fungicidal composition as claimed in claim 1 or claim 2, wherein triazole compound is Epoxiconazole and strobilurin compound is Pyraclostrobin.

7. The synergistic fungicidal composition as claimed in claim 1 or claim 2, wherein triazole compound is Epoxiconazole and strobilurin compound is Picoxystrobin.

8. The synergistic fungicidal composition as claimed in claim 1 or claim 2, wherein triazole compound is Prothioconazole and strobilurin compound is Trifloxystrobin.

9. The synergistic fungicidal composition as claimed in claim 1 or claim 2, wherein triazole compound is Prothioconazole and strobilurin compound is Picoxystrobin.

10. The synergistic fungicidal composition as claimed in claim 1 or claim 2, wherein triazole compound is Tricyclazole and strobilurin compound is Azoxystrobin.

11. The synergistic fungicidal composition as claimed in claim 1 or claim 2, wherein triazole compound is Tricyclazole and strobilurin compound is Trifloxystrobin.

12. The synergistic fungicidal composition as claimed in claim 1 or claim 2, wherein triazole compound is Tricyclazole and strobilurin compound is Picoxystrobin.

13. The synergistic composition as claimed in any one of the claims 1 to 12 wherein the formulation is selected from Water Dispersible Granules (WG or WDG), Wettable powder (WP), Soluble Concentrate (SL), Suspension Concentrate (SC), Emulsifiable Concentrate (EC), Microemulsion (ME), Oil Dispersion (OD), Capsule Suspension (CS), suspension emulsion or suspo emulsion (SE), Dispersible concentrate (DC), Dry – Spreadable Granule, GR – Soil applied Granule on inert or fertilizer carrier and ZC Formulation (Mixture of Capsule Suspension (CS) or Suspension Concentrate (SC)).