DESC:Synergistic Herbicide Combination of Ametryne and Diuron
FIELD OF THE INVENTION
The present invention relates to a synergistic herbicidal composition comprising the combination of triazine herbicide and substituted phenyl urea herbicide in EC / WDG / SC / SL / OD / OS / Solid Granules and other formulations in different percentages. More precisely, the subject matter of the present invention is a synergistic herbicidal composition based on a combination of ametryne and diuron optionally with at least one agriculturally acceptable excipient which will facilitate in the preparation of desired formulations. The present invention also relates to the process for the preparation of synergistic herbicidal composition thereof and use of this combination for contending weeds and protecting the crop from being affected with such weeds during its growth.
BACKGROUND OF THE INVENTION
Crop protection is the practice of protecting the crop yields from pests, weeds, plant diseases, and other organisms that damage agricultural crops, which is critical from early stages of crop development. Preventing weed growth in the entire crop cycle, i.e., from root development to maturing crop, leads to increased crop quality and yield. The control of weeds is extremely important in achieving high crop efficiency. Unwanted vegetation causes significant damage to vegetables, fields, cereal, fruit and other crops that compete with crops for nutrients, water, and sunlight leading to reduction in productivity, yield and quality of the crops. Herbicides help to minimize this damage by controlling the growth of weeds. The use of two or more appropriate active ingredient combinations in specific dose ratios leads to synergism in crop protection. In addition to this, often highly destructive weeds can be difficult to control and may develop resistance to commercial herbicides. Many products are commercially available for these purposes, but there is still a continues need to develop new herbicidal combinations which are more effective, less costly, less toxic, environmentally safer and have different sites of action.
The biggest challenge in field of crop protection is to reduce the dosage rate of active ingredients to diminish or circumvent environmental or toxicological effects without compromising on effective crop protection against weeds, in addition to long lasting and broad-spectrum of weed control. Another challenge is to reduce the excessive application of solo chemical compounds or herbicides which invariably helps in rapid selection of weeds and aid in developing natural or adapted resistance against the active compound in question.
Therefore, it is indeed necessary to use the herbicide combinations in lower doses, fast acting with the different mode of action that can provide long lasting control against broad spectrum weed control and check the resistance development in weeds. The composition should have high synergistic action, no cross resistance to existing herbicides, avoid excess loading of the toxicant to the environment and negligible impact to environmental safety. A need also exists for synergistic herbicidal compositions which could be physico-compatible formulations in the form of storage stable, safely packed, ready to use formulation.
OBJECT OF THE INVENTION
The principal object of the present invention is to provide an herbicide mixture or combination which solves at least one of the major problems discussed above like reducing the dosage rate, broadening the spectrum of activity, or combining activity with prolonged weed control and resistance management with improved environmental safety by reducing toxicity and residue deposit in soil and in crops.
The details of one or more embodiments of this disclosure are set forth in the accompanying description below and other features, objects, and advantages will be apparent from the description and the claims.

DESCRIPTION OF THE INVENTION
The present disclosure / specification refers to a synergistic herbicidal composition and the process for the preparation for crop protection.
The term “combination” can be replaced with the words “mixture” or “composition” defined or refers to as combining two or more active ingredients formulated in desired formulations.
The term “pesticide” as used in this specification refers to a substance or mixture of substances intended for preventing, destroying, repelling, or mitigating any pest or weeds which causes damage to the crop. Herbicides, insecticides, and fungicides are mainly used as pesticides which control weeds and insect pests and disease-causing pathogens respectively that eventually leads to high yield of crops.
The term “Herbicides” as used in this specification refers to a substance or mixture of substances used to kill, control or eliminate unwanted plants or vegetation, commonly known as weeds that cause economic damage to crops and ornamental plants.
The term “synergism” as used in this specification refers to the interaction between two or more active compounds or other factors to produce a combined effect greater than the sum of their separate effects. The present invention involves the mixture of two active ingredients which has increased efficacy when compared to individual use and admixture of those components.
The term “Ametryne” can be replaced or referred with the word “Ametryn”.
The term “plant nutrient” can be replaced with the word “nutrient” defined or refers to as chemical elements or compound which is necessary for plant growth and plant health. Plant nutrients are classified into two categories as:
Macronutrients: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), sulfur (S), magnesium (Mg) etc.
Micronutrients: iron (Fe), boron (B), manganese (Mn), zinc (Zn), copper (Cu), molybdenum (Mo), nickel (Ni) etc.
Conventional herbicides have typical spectrums and effects, are limited to certain weeds only and their controlling activities are sometimes poor and not satisfactorily maintained for prolonged period, and those satisfactory herbicidal effects cannot be practically achieved. Even though some herbicides may bear satisfactory herbicidal effects, they require improvements in respect of environment & health safety and are also demanded to achieve a high herbicidal effect at a smaller dosage and lack of resistance management.
We found that this objective in part or total can be achieved by the combination of active compounds defined at the outset. The present inventors have intensively studied to solve these problems and found that by combining herbicide composition having ametryne and diuron in different formulation and percentages have astonishing effects of controlling weeds and also by reducing amount of dosage than in a case of using an active compound alone and admixture of those compounds.
This combination can be developed in the form of Emulsifiable Concentrates (EC), Dispersible Concentrates (DC), Oil Dispersions (OD), Suspension Concentrates (SC), Soluble Liquids (SL), Suspoemulsion (SE), Emulsion Concentrates (EW), Microemulsions, Wettable Powders (WP), Water-Dispersible Granules (WG), Soluble Powders (SP), Granules (G), Oil Solutions (OS), Aqueous Suspensions (AS), Aqueous Solutions (AS), Microencapsulated Suspensions (ME), and Microencapsulated Emulsions (MEC), mixed formulation of Suspension Concentrate and Capsule Suspension (ZC) and other conventional formulation and with different percentages and can be used as an pre-emergent or early-post or post-emergent herbicidal combination for foliar applications or soil applications. This herbicidal combination can also effectively check the weeds and resistance development of weeds in various crops.
The present invention involves the mixture of two active ingredients which are classified under triazine herbicide and substituted phenyl urea herbicide and are described herein thereof.
Triazines are a group of synthetic herbicides that are widely used in agriculture to control the growth of broadleaf weeds and grasses. Ametryne is specifically classified as a triazine herbicide. Ametryne is a systemic broad spectrum, pre-emergent and post-emergent selective herbicide belonging to the specific s-triazines chemical group which inhibits photosynthetic and other enzymatic processes in weeds. Ametryn is effective against annual broadleaf weeds and grasses. Ametryn labeled for use on field corn, popcorn, pineapple, and sugarcane in the United States in 1964, ametryn (Evik®) is sold as an 80 W formulation. It also is used in banana, citrus, palm, coffee, potato and various other crops for general weed control. Most of the ametryn is used in sugarcane crops. It controls Dactyloctenium aegyptium, Digitaria sanguinalis, Cynodon dactylon, Ageratum conyzoides, Trianthema monogyna and Parthenium hysterophorus. It has both foliar and soil activity and this way it kills both newly emerged weeds and emerging new weeds from seeds. It effectively protects the sugarcane crop from early phase weed competition for longer period.
Another component the substituted urea herbicides (SUHs) belongs to one of the most important agricultural herbicide groups, wherein substitution of phenyl or sulfonyl chemical groups onto the urea structure differentiates the two of the main types of SUHs. The phenylureas act by inhibiting photosynthesis whereas the sulfonylurea herbicides inhibit the plant enzyme, acetolactate synthase, resulting in impaired branch chain amino acid synthesis. Diuron is a broad-spectrum, systemic, pre-emergence, early-post and post-emergence herbicide belonging to the substituted phenyl urea herbicide particularly 3-(3,4-substituted-phenyl)-1,1-dimethylurea class. Diuron acts by inhibiting photosynthesis in plants primarily affects the photosystem II complex, which is responsible for capturing light energy and driving the synthesis of carbohydrates in plants. By disrupting this process, diuron prevents the growth and development of targeted weeds, ultimately leading to their death. It controls selected grasses, sedges and broad leaf weeds in cotton, banana, rubber, maize, citrus, sugarcane and grapes crop etc. viz., Amaranthus spp, Chenopodium album, Convolvulas arvensis Setaria glauca, Digitaria sp, Portulaca oleracea, Xanthium strumerium, Anagallis arvensis, Asphodelus temifolius, Euphorbia sp, Visia sativa, Paspalum conjugatum, Commelina benghalensis, Dactyloctenium, Chloris barbata, Eragrostis zeylenica, Cyperus iria, Echinochloa spp, Eleusine sp, Phyllanthus niruri, Tribulus Terristris, Commelina nudiflora, Cocumis trigonus, Cynotis spp, Cleome viscose, Alternanthera echinata, Argemone maxicana, Ipomoea spp, Xanthium strumerium, Fumeria parviflora, Asphodelus tenuifolius, Medicago denticulata, Eleusine aegyptia.
The present invention provides a novel synergistic herbicide composition having ametryne and diuron and purpose thereof. The synergy of herbicidal composition has the main effective components of ametryne and diuron. This herbicidal composition acts by interference/inhibition of photosynthetic and associated enzymatic processes can generate efficient synergism as a pre-emergence or early-post or post-emergent herbicide and can enable broad spectrum satisfactory weed control and protect the several crop from sedges, grasses and broad leaf weeds for prolonged period of time at lower dose with no phytotoxic effect. It also prevents the weed crop from rejuvenation and further regeneration.
The first embodiment of the present invention provides a synergistic herbicidal composition comprising:
at least one triazine herbicide; and
at least one substituted phenyl urea herbicide.
First aspect of the first embodiment, the triazine herbicide is selected from but not limited the group comprising ametryn, atrazine, cyanazine, desmetryn, metribuzin, prometon, prometryn, propazine, simazine, terbutryn and terbuthylazine; preferably ametryn.
Second aspect of the first embodiment, the substituted phenyl urea herbicide is selected from but not limited the group comprising anisuron, buturon, chlorbromuron, chlortoluron, chloroxuron, difenoxuron, diuron, fenuron, fluometuron, isoproturon, linuron, methiuron, metobromuron, metoxuron, monuron, neburon, parafluron, siduron, tebuthiuron, tetrafluron and thidiazuron; preferably diuron.
Third aspect of the first embodiment, synergistic herbicidal composition comprising a combination of triazine herbicide and substituted phenyl urea herbicide; wherein triazine herbicide and substituted phenyl urea herbicide are present in the weight ratio of (1-80): (1-80); preferably in the ratio of (1-40): (1-40).
The second embodiment of the present invention provides a synergistic herbicidal composition comprising:
at least one triazine herbicide;
at least one substituted phenyl urea herbicide; and
at least one nutrient.
First aspect of the second embodiment, the triazine herbicide is selected from but not limited the group comprising ametryn, atrazine, cyanazine, desmetryn, metribuzin, prometon, prometryn, propazine, simazine, terbutryn and terbuthylazine; preferably ametryn.
Second aspect of the second embodiment, the substituted phenyl urea herbicide is selected from but not limited the group comprising anisuron, buturon, chlorbromuron, chlortoluron, chloroxuron, difenoxuron, diuron, fenuron, fluometuron, isoproturon, linuron, methiuron, metobromuron, metoxuron, monuron, neburon, parafluron, siduron, tebuthiuron, tetrafluron and thidiazuron; preferably diuron.
Third aspect of the second embodiment, the nutrient selected from but not limited to the group comprising zinc, iron, magnesium, phosphorus, manganese, chlorine, nickel, calcium and/or potassium; preferably zinc.
Fourth aspect of the second embodiment, synergistic herbicidal composition comprising a combination of triazine herbicide and substituted phenyl urea herbicide; wherein triazine herbicide and substituted phenyl urea herbicide are present in the weight ratio of (1-80): (1-80); preferably in the ratio of (1-40): (1-40).
The third embodiment of the present invention provides a synergistic herbicidal composition comprising:
at least one triazine herbicide;
at least one substituted phenyl urea herbicide;
at least one nutrient; and
at least one agriculturally acceptable excipient.
First aspect of the third embodiment, the triazine herbicide is selected from the group comprising ametryn, atrazine, cyanazine, desmetryn, metribuzin, prometon, prometryn, propazine, simazine, terbutryn and terbuthylazine; preferably ametryn.
Second aspect of the third embodiment, the urea herbicide is selected from the group comprising anisuron, buturon, chlorbromuron, chlortoluron, chloroxuron, difenoxuron, diuron, fenuron, fluometuron, isoproturon, linuron, methiuron, metobromuron, metoxuron, monuron, neburon, parafluron, siduron, tebuthiuron, tetrafluron and thidiazuron; preferably diuron.
Third aspect of the third embodiment, the nutrient selected from but not limited to the group comprising zinc, iron, magnesium, phosphorus, manganese, chlorine, nickel, calcium and/or potassium; preferably zinc.
Fourth aspect of the third embodiment, synergistic herbicidal composition comprising a combination of triazine herbicide and substituted phenyl urea herbicide; wherein triazine herbicide and substituted phenyl urea herbicide are present in the weight ratio of (1-80): (1-80); preferably in the ratio of (1-40): (1-40).
Fifth aspect of the third embodiment, agriculturally acceptable excipient selected from but not limited to group comprising of liquid medium, surfactant, stabilizer, anti-freezing agent, antifoaming agent, anticaking agent, dispersing agent and adjuvant. These are selected according to the respective types of formulation requirements, and which will facilitate in the preparation different formulations.
A further aspect of the third embodiment, liquid medium acts as a carrier for the active ingredient and provides a stable environment for suspension.
Further aspect of the third embodiment, surfactant includes wetting agent and emulsifier.
Further aspect of the third embodiment, wetting agent selected from but not limited to alkyl aryl sulfonates, alkyl phenol ethoxylates, alkyl polyglucosides, polyethylene glycol esters, polysorbate, polyethylene oxide (PEO), ethoxylated fatty alcohols, ethoxylated vegetable oils, ethoxylated sorbitan esters, propylene glycol esters, sodium lauryl sulfate, cocoamidopropyl betaine and block copolymers selected from the but not limited to styrene-butadiene block copolymer (SBS), butyl based block copolymer, poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO), polystyrene-poly(ethylene oxide) (PS-PEO), poly(butadiene)-poly(styrene) (PB-PS), poly(methyl methacrylate)-poly(butadiene)-poly(methyl methacrylate) (PMMA-PB-PMMA), poly(caprolactone)-poly(ethylene glycol) (PCL-PEG) and poly(ethylene glycol)-poly(propylene glycol)-poly(ethylene glycol) (PEG-PPG-PEG); preferably butyl based block copolymer or sodium lauryl sulfate.
Further aspect of the third embodiment, stabilizer includes antioxidant, chelating agent, pH adjusters, UV absorber, stabilizing polymers and inert filler.
Further aspect of the third embodiment, inert filler selected from but not limited to kaolin clay, attapulgite clay, bentonite, montmorillonite, acid white clay, pyrophyllite, talc, diatomaceous earth and calcite, china clay, corn rachis powder, walnut husk powder, urea, calcium carbonate, ammonium sulfate and silicon oxide; preferably china clay.
Further aspect of the third embodiment, anti-freezing agent selected from but not limited to ethylene glycol, propylene glycol, glycerol, calcium chloride, sodium acetate, potassium acetate and urea; preferably propylene glycol.
Further aspect of the third embodiment, antifoaming agent selected from but not limited to silicone-based antifoams, polyethylene glycol-based antifoams, mineral oil-based antifoams, ethylene glycol-based antifoams, polysorbate-based antifoams, dimethicone-based antifoams, polypropylene glycol-based antifoams, vegetable oil-based antifoams, alkyl siloxane-based antifoams and fatty acid-based antifoams; preferably alkyl siloxane-based antifoams; more preferably siloxane polyalkyleneoxide.
Further aspect of the third embodiment anticaking agent selected from but not limited to silica-based compounds includes silicon dioxide (silica), precipitated silica (amorphous form of silicon dioxide), calcium silicate, magnesium stearate, sodium aluminosilicate, potassium aluminium silicate, tricalcium phosphate, sodium ferrocyanide, calcium carbonate, diatomaceous earth and sodium bicarbonate; preferably precipitated silica.
Further aspect of the third embodiment, dispersing agent selected from but not limited to polyethylene glycol, polysorbate, poly acrylate, poly(methyl methacrylate), polyvinyl alcohol, poly ethoxylated alcohol, poly ethoxylated fatty acids, polyacrylic acid, polyvinylpyrrolidone, alkyl sulfonates, aryl sulfonates, alkyl sulfates, sodium tripolyphosphate, sodium dodecyl sulfate, sodium lignosulfonate, sodium carboxymethyl cellulose, hydroxypropyl methylcellulose, sorbitan esters (e.g., sorbitan monolaurate, sorbitan monooleate), gum arabic and carbomer and/or their comb polymers; preferably sodium naphthalene sulfonate, sodium lignosulfonate or combination.
Further aspect of the third embodiment, adjuvant includes but not limited to spreader, sticker, penetrant, drift control agent, preservative, buffering agent, thickener, compatibility agent, binders and safener.
Further aspect of the third embodiment, thickener selected from but not limited to polysaccharides / carboxymethyl cellulose / bentonite clay, hydroxy propyl cellulose montmorillonite, bentonite, magnesium aluminium silicate and attapulgite; preferably water-soluble polysaccharides.
Further aspect of the third embodiment, antibacterial agent selected from but not limited to triclosan, triclocarban, clotrimazole, miconazole, copper-based compounds, chlorothalonil, benzisothiazolin-3-one (BIT), 5-chloro-2-methyl-4-isothiazolin-3-one, 2-methyl-4-isothiazolin-3-one (MIT), octylisothiazolinone (OIT) and dodecylbenzenesulfonic acid sodium salt (DBSA); preferably benzisothiazolin-3-one (BIT).
The fourth embodiment of the present invention provides a synergistic herbicidal composition comprising:
ametryn;
diuron; and
nutrient.
First aspect of the fourth embodiment, synergistic herbicidal composition comprising a combination of ametryn and diuron; wherein ametryn and diuron are present in the weight ratio of (1-80): (1-80); preferably in the ratio of (1-40): (1-40); more preferably in the ratio of (1-40): (1-20).
Second aspect of the fourth embodiment, the nutrient selected from but not limited to the group comprising zinc, iron, magnesium, phosphorus, manganese, chlorine, nickel, calcium and/or potassium; preferably zinc.
Further aspect of the fourth embodiment, the composition of fourth embodiment comprising at least one agriculturally acceptable excipient thereof which is / are used in preparation desired formulation.
The further aspect of the fourth embodiment, the composition of fourth embodiment is formulated as suspension concentrates (SC).
The further aspect of the fourth embodiment, the composition of fourth embodiment is formulated as wettable powder (WP).
The fifth embodiment of the present invention provides a synergistic herbicidal composition comprising:
ametryn;
diuron; and
zinc.
First aspect of the fifth embodiment, synergistic herbicidal composition comprising a combination of ametryn and diuron; wherein ametryn and diuron are present in the weight ratio of (1-80): (1-80); preferably in the ratio of (1-40): (1-40); more preferably in the ratio of (1-40): (1-20).
A further aspect of the fifth embodiment, the composition of fifth embodiment comprising at least one agriculturally acceptable excipient thereof which is / are used in preparation desired formulation.
The further aspect of the fifth embodiment, the composition of fifth embodiment is formulated as suspension concentrates (SC).
The further aspect of the fifth embodiment, the composition of fifth embodiment is formulated as wettable powder (WP).
The sixth embodiment of the present invention provides a synergistic herbicidal composition comprising:
ametryn;
diuron;
zinc;
sodium lauryl sulfate;
china clay;
precipitated silica;
sodium naphthalene sulfonate; and
sodium lignosulfonate.
First aspect of the sixth embodiment, synergistic herbicidal composition comprising a combination of ametryn and diuron; wherein ametryn and diuron are present in the weight ratio of (1-80): (1-80); preferably in the ratio of (1-40): (1-40); more preferably in the ratio of (1-40): (1-20).
The further aspect of the sixth embodiment, the composition of sixth embodiment is formulated as wettable powder (WP).
The seventh embodiment of the present invention provides a synergistic herbicidal composition comprising:
ametryn;
diuron;
zinc;
butyl based block copolymer;
propylene glycol;
siloxane polyalkyleneoxide;
comb polymer;
polysaccharides; and
benzisothiazolin-3-one.
First aspect of the seventh embodiment, synergistic herbicidal composition comprising a combination of ametryn and diuron; wherein ametryn and diuron are present in the weight ratio of (1-80): (1-80); preferably in the ratio of (1-40): (1-40); more preferably in the ratio of (1-40): (1-20).
Further aspect of the seventh embodiment, the composition of seventh embodiment is formulated as suspension concentrates (SC).
Another embodiment of present invention provides a process for the preparation of an herbicidal formulation comprising:
add china clay into the pre blender.
add ametryn and diuron into the same blender and mix well until a homogenous mixture is obtained,
add micronutrient, wetting agent, dispersing agent, and anticaking agent into the above mixture and mix well.
after mixing the above material, optionally mill or grind to get the desired particle size and remove any oversized particles or agglomerate,
Then material is transferred into the post blender and mixed well until a homogenous mixture is obtained.
Pack the formulation and seal it.
Another embodiment of the present invention, the synergistic herbicidal composition of present invention used in defined dose rations shows effective weed control in broad spectrum of weeds and increases the crop yield, quality, lesser dose rations and shows no phytotoxicity compared to single components, admixture of those two component and other available market standards. By this the herbicidal composition of present invention archives synergistic effect along with economical value product and improved environmental safety by reducing toxicity and residue deposit in soil and in crops.
Another embodiment of the present invention, a method of controlling annual grasses, sedges and broad leaf weeds comprising synergistic herbicidal composition applying to the location of weed in effective amount.
Another embodiment of the present invention, the herbicidal composition obtained from the present invention is used to control broadleaf weeds, sedges and annual grasses in rice, wheat, fruits, roots, tubers, vegetables, maize, grains, sugarcane, cereals, field crops and various other crops for general weed control.
Another embodiment of the present invention, the herbicidal composition obtained from the present shows synergistic effects of better weed control with minimum herbicidal resistance and improved crop yield and quality.
Another embodiment of the present invention, the synergistic herbicidal composition can be applied to pre-emergence, early post-emergence or post-emergence stages by conventional spraying methods, such as foliar application etc., over the target areas of weeds or vegetation at same time avoiding excessive drift or runoff of the composition securing thorough coverage.
Advantages of Present invention:
The synergistic herbicidal composition of present invention can be used in pre-emergence, early post-emergence or post-emergence stages for effective weed control to increase the yield of crops compare to single component of composition and market standards. This will help the farmers in controlling weeds in the entire crop cycle.
The novel, innovative and synergistic herbicidal composition of present invention provides better weed management in agricultural and non-agricultural fields.
The synergistic herbicidal composition of present invention is effective against broader spectrum of unwanted weeds compared to single component and market standards without any environmental pollution.
The synergistic herbicidal composition of the present invention avoids or reduces the damage of crops and shows no phytotoxicity.
The best mode of carrying present invention is described in the below given examples. These examples are merely for illustrative purposes only, not to determine the scope of the invention and in no way limit the scope or spirit of the present invention.
EXAMPLES:
EXAMPLE 1: SUSPENSION CONCENTRATES (SC) FORMULATION OF SYNERGISTIC HERBICIDAL COMPOSITION OF THE PRESENT INVENTION:
TABLE 1:
S. No Ingredient Weight / Weight %
1 Ametryn 30
2 Diuron 15
3 Zinc 1
4 Block copolymer 2.5
5 Propylene Glycol 5
6 Siloxane polyalkyleneoxide 0.5
7 Comb polymer 2.5
8 Polysaccharides 0.3
9 Benzisothiazolin-3-one 0.1
Water QS
Total 100

EXAMPLE 1.1: WETTABLE POWDER FORMULATION OF SYNERGISTIC HERBICIDAL COMPOSITION OF THE PRESENT INVENTION:
TABLE 2:
S. No Ingredient Weight/ Weight %
1 Ametryn 30
2 Diuron 15
3 Zinc 1
4 Sodium lauryl sulfate 10
5 Precipitated silica 10
6 Sodium alkyl naphthalene sulfonate 3
7 Sodium lignosulfonate 2
8 China clay QS
Total 100
EXAMPLE 2.1: PROCESS FOR PREPARATION OF SUSPENSION CONCENTRATES (SC) OF SYNERGISTIC HERBICIDAL COMPOSITION OF THE PRESENT INVENTION:
Add water, diuron and ametryn into the pre blender in the above-mentioned ratios and mix well until a homogenous mixture is obtained. Add zinc, wetting agent, anti-freezing, dispersing agent, anti-bacterial agent and antifoaming agent in the above-mentioned ratios into the above mixture and mix well. Optionally mill or grind to get the desired particle size. Then add thickener to the above obtained material mixed well until to get the homogenous mixture with desired viscosity.
EXAMPLE 2.2: PROCESS FOR PREPARATION OF WETTABLE POWDER OF SYNERGISTIC HERBICIDAL COMPOSITION OF THE PRESENT INVENTION:
Add china clay, diuron and ametryn into the pre blender in the above-mentioned ratios and mix well until a homogenous mixture is obtained. Add zinc, wetting agent, dispersing agents, anticaking agent and superior absorption agent in the above-mentioned ratios into the above mixture and mix well. Then material is transferred into the post blender and mixed well until a homogenous mixture is obtained.
EXAMPLE 3: BIO EFFICACY AND PHYTOTOXICITY TESTS OF THE PRESENT INVENTION
Parameters of Observations:
The weeds controlled in each treatment are calculated as percentage of weed controlled over untreated or controlled plot. The percentage of weed control is calculated by the following formula:–
% weed control = (No.of weeds in control plot-No.of weeds in treated plot )/(No.of weeds in control plot)× 100
The effect of these herbicides along with zinc when applied on sugarcane crop is assessed based on the yield parameters like the cane yield (tonnes per hectare), cane length (cms), cane diameter (cms) and number of millable shoots (000’ ha-1) and sugar content in percentage Brix. All these parameters define the crop quality and help to conclude phyto-tonic effects of herbicides on the crop.
Results:
The different types and species of weeds found in this experiment in sugarcane field were enlisted below,
Grasses: Digitaria sanguinalis and Chloris barbata.
Broad Leaved Weeds: Trianthema portulacastrum, Amaranthus viridis and Portulaca oleracea.
Sedges: Cyprus iria.


Table 3. Efficacy of diuron 15% + ametryne 30% (along with Zn @80ml/ha) SC formulation against grassy weeds:
Treatments Dose / Ha
(Gm/L) Pre-Count 7 DAS 14 DAS 28 DAS 35 DAS Average Percent Weed Control
D.s C.b D.s C.b D.s C.b D.s C.b D.s C.b D. s C. b D. s C. b
Diuron + ametryne SC 3750 49 13 11 6 5 3 4 3 3 3 5.75 3.75 90.57 80.26
Diuron + ametryne SC 5000 43 11 0 0 0 0 1 2 2 2 0.75 1 98.77 94.74
Diuron + ametryne SC 6250 51 16 0 0 0 0 0 1 1 1 0.25 0.5 99.59 97.37
Diuron 80% SC 4000 47 18 27 9 19 7 6 4 6 6 14.5 6.5 76.23 65.79
Ametryne 80% WDG 2500 43 17 11 4 6 2 8 5 12 8 9.25 4.75 84.84 75.00
Mesotrione 2.27 + atrazine 22.7% SC 3500 41 15 9 2 7 3 5 4 9 7 7.5 4 87.70 78.95
Topramezone 10g/L + atrazine 300g/L SC 3000 49 10 7 2 5 3 5 2.5 19 6 9 3.37 85.25 82.24
Untreated control -- 42 17 46 19 56 19 71 19 71 19 61 19

D.s - Digitaria sanguinalis
C.b - Chloris barbata
DAS- Days After Spray
The results presented in table-1 indicated that the grassy weed population before spraying ranged between 41-51 weeds/sq.m in D. sanguinalis and 10-18 weeds/sq.m in C. barbata. After the application of different herbicide treatments, the synergistic herbicide combination of diuron + ametryne when applied at 5000gm/ha and 6250 gm/ha showed maximum reduction in weed population of 0 weeds/m2 at 7, 14 days after spraying and 1-2 weeds/m2 at 28 and 35 days after spraying in both weed species viz., D. sanguinalis and C. barbata. The average weeds population after the entire spraying was recorded, the lowest in diuron + ametryne @ 6250 ml/ha with 0.25 D.sanguinalis weeds/m2 and 0.5 C. barbata weeds/m2 followed by diuron + ametryne @ 5000 ml/ha with 0.75 D.sanguinalis weeds/m2 and 1 C. barbata weeds/m2 which were performing superior to market standards as well as individuals. The percentage of weed control recorded in all the treatments proved that diuron + ametryne when applied at 6250 ml/ha showed the maximum weed control of 99.59% (D.sanguinalis) and 97.37% (C. barbata). When applied at 5000 ml/ha the results recorded that 98.77% (D.sanguinalis) and 94.74% (C. barbata) of weed control there by proving that these active ingredients when applied in combination at the doses of 6250, 5000 gm/ha showed synergistic activity and weeds control superior to market standard. Even the treatment diuron + ametryne when applied at 3750 ml/ha (lowest dose of the herbicide combination) also showed weed control of 90.57% (D. sanguinalis) and 80.26% (C. barbata) which is also superior to the market standard.
Table 2. Efficacy of diuron 15% + ametryne 30% (along with Zn @80ml/ha) SC formulation against broad leaved weeds:
Treatments Dose /Ha
(Gm/L) Pre-Count 7 DAS 14 DAS 28 DAS 35 DAS Average Percent Weed Control
T.p P.o A.v T.p P.o A.v T.p P.o A.v T.p P.o A.v T.p P.o A.v T.p P.o A. v T.p P.o A. v
Diuron + ametryne SC 3750 123 18 26 6 3 4 3 3 3 6 5 6 23 11 15 9.5 5.5 7 93.58 81.67 84.18
Diuron + ametryne SC 5000 141 23 26 0 0 0 0 0 0 2 5 9 10 9 10 3 3.5 4.75 97.97 88.33 89.27
Diuron + ametryne SC 6250 136 16 35 0 0 0 0 0 0 0 1 1 10 4 6 2.5 1.25 1.75 98.31 95.83 96.05
Diuron 80% SC 4000 119 24 37 41 8 10 27 6 6 6 2 3 16 7 9 22.5 5.75 7 84.80 80.83 84.18
Ametryne 80% WDG 2500 110 24 31 23 9 7 15 2 11 13 2 2 11 12 8 15.5 6.25 7 89.53 79.17 84.18
Mesotrione 2.27 + atrazine 22.7% SC 3500 124 16 30 14 6 3 19 11 10 23 16 12 20 15 12 19 12 9.25 87.16 60.00 79.10
Topramezone 10g/L + atrazine 300g/L SC 3000 132 19 37 95 11 26 84 9 19 24 5 14 12 2 6 53.7 6.75 16.25 63.68 77.50 63.28
Untreated control -- 126 23 32 139 27 39 151 31 46 151 31 46 151 31 46 148 30 44.25

T. p - Trianthema portulacastrum
P. o - Portulaca oleracea
A. v - Amaranthus viridis
DAS- Days After Spray
The broad-leaved weeds species identified in the sugarcane field were Trianthema portulacastrum, Portulaca oleracea and Amaranthus viridis. The results presented in table-2 indicated that the population before spraying ranged between 110-141 weeds/m2 in T. portulacastrum, 16-24 weeds/m2 in P. oleracea and 26-37 weeds/ m2 in A. viridis. After the application of different herbicide treatments, the herbicide combination of diuron + ametryne when applied at 5000gm/ha and 6250 gm/ha showed maximum reduction in weed population of 0 weeds/m2 at 7, 14 days after spraying and 0-2 weeds/m2, 1-5 weeds/m2 and 1-9 weeds/m2 at 28 and 35 days after spraying in the three weed species viz., T. portulacastrum, P. oleracea and A. viridis. The average weeds population after the entire spraying was recorded the lowest in diuron + ametryne @ 6250 ml/ha with 2.5 T. portulacastrum weeds/m2, 1.25 P. oleracea weeds/m2 and 1.75 A. viridis; followed by diuron + ametryne @ 5000 ml/ha with 3 T. portulacastrum weeds/m2, 3.5 P. oleracea weeds/m2 and 4.75 A. viridis which were performing superior to market standards. The percentage of weed control recorded in all the treatments proved that diuron + ametryne when applied at 6250 ml/ha showed the maximum weed control 98.31% (T. portulacastrum), 95.83% (P. oleracea) and 96.07% (A. viridis). When applied at 5000 ml/ha results recorded 97.97% (T. portulacastrum) 88.33% (P. oleracea) and 89.27% (A. viridis) of weed control there by proving that these active ingredients when applied in combination at the doses of 6250, 5000 gm/ha showed synergistic activity and weeds control superior to market standard. Even the treatment diuron + ametryne when applied at 3750 ml/ha (lowest dose of the herbicide combination) also showed weed control of 93.58% (T. portulacastrum) 81.67% (P. oleracea) and 84.18% (A. viridis) which is also superior to the market standard.

Table 3. Efficacy of diuron 15%+ ametryne 30% (along with Zn @80ml/ha) SC formulation against Sedges (Cyperus iria):
Treatments Dose / Ha
(gm/L) Pre-Count 7 DAS 14 DAS 28 DAS 35 DAS Average Percent Weed Control
Diuron + ametryne SC 3750 17 2 4 9 14 7.25 76.80
Diuron + ametryne SC 5000 12 0 2 7 11 5 84.00
Diuron + ametryne SC 6250 16 0 0 4 12 4 87.20
Diuron 80% SC 4000 14 10 11 12 14 11.75 62.40
Ametryne 80% WDG 2500 18 3 8 12 20 10.75 65.60
Mesotrione 2.27 + atrazine22.7% SC 3500 13 5 11 15 20 12.75 59.20
Topramezone10g/L + atrazine300g/L SC 3000 17 11 12 12 15 12.5 60.00
Untreated Control -- 15 20 25 35 45 31.25
C.i – Cyperus iria
DAS-Days After Spray
The sedge weeds species were very well known for their mode of propagation to be through rhizomes and the very same character of sedges made them to be very difficult to control and took their place as one of the most noxious weeds in all the different weed species available in the field level. The sedges found in the sugarcane field in this experiment belong to a single species i.e., Cyperus iria. The results presented in table 3, the population before spraying ranged between 12-18 weeds/m2. After the application of different herbicide treatments, the herbicide combination of diuron + ametryne when applied at 6250 gm/ha and 5000gm/ha showed maximum reduction in weed population of 0 weeds/m2 at 7 days after spraying, 0 weeds/m2 in diuron + ametryne @ 6250 ml/ha and 2 weeds/m2 in diuron + ametryne @ 5000 ml/ha at 14 days after spraying and 4 weeds/m2, 12 weeds/m2 in diuron + ametryne @ 6250 ml/ha at 28DAS, 35DAS and 7 weeds/m2, 11 weeds/m2 in diuron + ametryne @ 5000 ml/ha at 28DAS, 35DAS in the weed species viz., C. iria. The average weeds population after the entire spraying was recoded the lowest in diuron + ametryne @ 6250 ml/ha with 4 weeds/m2 and followed by diuron + ametryne @ 5000 ml/ha with 5 weeds/m2 which were performing superior to market standards. The percentage weed control recorded in all the treatments proved that diuron + ametryne when applied at 6250 ml/ha showed the maximum weed control 87.20%. When applied at 5000 ml/ha results recorded 84.00% of weed control. The weeds when sprayed individually recorded low percent weed control viz.,62.40% and 65.60% there by proving that these active ingredients when applied in combination at the doses of 6250, 5000 gm/ha showed synergistic activity and weeds control superior to market standard. Even the treatment diuron + ametryne when applied at 3750 ml/ha (lowest dose of the herbicide combination) also showed weed control of 76.80% which is also superior to the market standard.
Table 4. Phytotoxicity of diuron 15% + ametryne 30% (along with Zn @80ml/ha) SC formulation on Sugarcane crop:
Treatments DAS Visual Rating Scale
Yellowing Necrosis Wilting Vein
Clearing Leaf Tip /
Margin Dying Stunting / Dwarfing
Diuron + ametryne SC @3750 gm/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Diuron + ametryne SC @5000 ml/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Diuron + ametryne SC @6250 ml/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Diuron 80% SC @4000 ml/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Ametryne 80%WDG @2500 ml/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Mesotrione 2.27 + atrazine 22.7% SC @3500 ml/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Topramezone 10g/L + atrazine 300g/L SC @3000 ml/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Untreated Control 5 0 0 0 0 0 0
10 0 0 0 0 0 0

The phytotoxicity effect of the herbicide combination i.e., diuron + ametryne SC on sugarcane crop was tested after 5 and 10 days after spraying. The crop was examined for symptoms like yellowing, necrosis, wilting, vein clearing, leaf tip or leaf margin dying and stunting or dwarfing of plants. After thorough observations, it was concluded that the crop did not show any symptoms of phytotoxicity. Therefore, the present herbicide combination can be considered a safe combination.
Table 5. Effect of diuron 15% + ametryne 30% (along with Zn @80ml/ha) SC formulation on crop quality:
Treatments Dose / Ha (Gms/Ml) Cane
Yield (T/Ha) Cane Length Cane
Diameter
(Cms) No. Millable
Shoots - 000
Per Ha Sugar Content
(% Brix)

Diuron + Ametryne SC 3750 90 273 2.65 105 19.88
Diuron + Ametryne SC 5000 94 298 2.85 109 20
Diuron + Ametryne SC 6250 114 316 3.5 118 21.8
Diuron 80% SC 4000 83 224 2.5 100 18
Ametryne 80% WDG 2500 80 235 2.7 98 18
Mesotrione 2.27 + Atrazine 22.7% SC 3500 66 195 2.2 95 18.89
Topramezone 10g/L + Atrazine 300g/L SC 3000 75 216 2.4 89 17
Untreated Control -- 54 183 1.8 72 13
The cane yield among different herbicide treatments, the synergistic herbicide combination of diuron + ametryne when applied at 6250ml/ha, 5000ml/ha and 3750ml/ha recorded the highest cane yield of 84 t/ha, 82 t/ha and 80 t/ha which shows that the herbicidal combination when applied at three different doses viz., low, medium, and high have similar positive effect on the cane yield of sugarcane crop. While the same herbicides when applied alone i.e., Diuron 80 SC @ 4000ml/ha gave 73 t/ha and ametryne 80 WDG @ 2500 ml/ha gave 70 t/ha yield. The cane length and cane diameter were also recorded highest as in the case of cane yield. The highest length of cane was showed in the treatment with diuron + ametryne SC @6250ml/ha with 316cm long cane followed by 298cm long cane with the treatment of diuron + ametryne SC @5000ml/ha. Similar trend was observed in cane diameter with 3.5cm and 2.85cm diameter in diuron + ametryne when applied at 6250ml/ha and 5000ml/ha. The number of millable shoots (000’ ha-1) was also recorded the highest as 118, 109 in diuron + ametryne combination when applied at 6250ml/ha, 5000ml/ha. The sugar content was recorded highest in diuron + ametryne combination when applied at 6250ml/ha, 5000ml/ha and 3250ml/ha viz., 21.80%, 20% and 19.88% respectively. It is evident based on the results that the herbicide combination has a synergistic effect when applied together especially with the inclusion of zinc in the combinational mixture improved the cane quality in the current case. It has phytotonic effect on sugarcane crops apart from efficiently controlling the weed populations.

Table 6. Efficacy of diuron 20% + ametryne 35% (along with Zn @80ml/ha) SC formulation against grassy weeds:
Treatments Dose / Ha
(gm/L) Pre-Count 7 DAS 14 DAS 28 DAS 35 DAS Average Percent Weed Control
D.s C.b D.s C.b D.s C.b D.s C.b D.s C.b D. s C. b D. s C. b
Diuron + Ametryne SC 3065 52 19 31 10 21 17 14 11 26 6 23 11 66.67 53.68
Diuron + Ametryne SC 4000 53 15 23 11 12 19 16 10 21 4 18 11 73.91 53.68
Diuron + Ametryne SC 5100 51 19 21 16 15 12 4 6 12 3 13 6.75 81.16 71.58
Diuron 80% SC 4000 49 16 33 12 21 18 15 4 17 6 21.5 10 68.84 57.89
Ametryne 80%WDG 2500 47 17 31 14 17 10 12 5 17 8 19.25 9.25 72.10 61.05
Mesotrione 2.27 + Atrazine 22.7% SC 3500 46 19 36 17 19 10 11 4 17 7 20.75 9.5 69.93 60.00
Topramezone 10g/L + Atrazine 300g/L SC 3000 51 15 45 15 30 9 19 2.5 25 6 29.75 8.125 56.88 65.79
Untreated Control -- 52 18 61 24 69 33 71 19 75 19 69 23.75
D.s - Digitaria sanguinalis
C. b - Chloris barbata
DAS-Days After Spray
The grassy weed population before spraying ranged between 46-53 weeds/sq.m in D. sanguinalis and 15-19 weeds/sq.m in C. barbata. After the application of different herbicide treatments, the herbicide combination of diuron + ametryne when applied at 5100 ml/ha showed maximum reduction in weed population of 21,16 weeds/m2 at 7 days after spraying and 15,12 weeds/m2 at 14 days after spraying and 4,6 weeds/m2 at 28 days after spraying and 12, 3 weeds/m2 at 35 days after spraying in both weed species viz., D. sanguinalis and C. barbata. The average weeds population after the entire spraying was recorded the lowest in Diuron + Ametryne @ 5100 ml/ha with 13 D.sanguinalis weeds/m2 and 6.75 C. barbata weeds/m2 followed by diuron + ametryne @ 4000 ml/ha with 18 D.sanguinalis weeds/m2 and 11 C. barbata weeds/m2 which were performing superior to market standards. The percentage weed control recorded in all the treatments proved that diuron + ametryne when applied at 5100 ml/ha were showing maximum weed control 81.16% (D.sanguinalis) and 71.98% (C. barbata) and at 4000 ml/ha recorded 73.91% (D.sanguinalis) and 53.58% (C. barbata) of weed control.
Table 7. Efficacy of diuron 20% + ametryne 35% (along with Zn @80ml/ha) SC formulation against broad leaved weeds.
Treatments Dose
/ Ha
(Gm/L) Pre-Count 7 DAS 14 DAS 28 DAS 35 DAS Average Percent Weed Control
T.p P.o A.v T.p P.o A.v T.p P.o Av T.p P.o A.v T.p P.o A.v T.p P.o A.v T.p P.o A.v
Diuron + ametryne SC 3065 134 28 31 102 19 19 4 4 3 7 7 6 23 11 14 34 10.25 10.5 78.52 75.15 79.81
Diuron + ametryne SC 4000 141 23 30 93 15 16 1 1 2 4 7 9 18 9 13 29 8 10 81.67 80.61 80.77
Diuron + ametryne SC 5100 136 25 29 85 10 11 0 0 1 2 4 3 12 6 8 24.75 5 5.75 84.36 87.88 88.94
Diuron80%SC 4000 137 24 34 91 21 20 19 7 6 8 4 8 26 9 9 36 10.25 10.75 77.25 75.15 79.33
Ametryne 80% WDG 2500 140 24 31 113 23 23 15 3 11 15 4 15 31 12 23 43.5 10.5 18 72.51 74.55 65.38
Mesotrione2.27 + atrazine 22.7% SC 3500 129 26 35 124 24 27 19 15 10 25 19 12 30 17 26 49.5 18.75 18.75 68.72 54.55 63.94
Topramezone 10g/L + atrazine 300g/L SC 3000 141 29 33 120 26 26 84 11 19 26 15 24 32 12 31 65.5 16 25 58.61 61.21 51.92
Untreated Control -- 138 29 32 145 32 39 151 35 49 162 47 56 175 51 64 158.25 41.25 52

T. p - Trianthema portulacastrum
P. o - Portulaca oleracea
A. v - Amaranthus viridis
DAS- Days After Spray
The broad-leaved weeds species identified in the sugarcane field were Trianthema portulacastrum, Portulaca oleracea and Amaranthus viridis. The population before spraying ranged between 129-141 weeds/m2 in T. portulacastrum, 23-29 weeds/m2 in P. oleracea and 29-35 weeds/m2 in A. viridis. After the application of different herbicide treatments, the herbicide combination treatment of diuron + ametryne when applied at 5100 gm/ha showed maximum reduction in weed population of 85, 10, 11 weeds/m2 at 7 days after spraying, 0, 0, 1 at 14 days after spraying and 2, 4 and 3 weeds/m2 at 28 days after spraying and 12, 6 and 8 weeds/m2 at 35 days after spraying in the three weed species viz., T. portulacastrum, P. oleracea and A. viridis. The average weeds population after the entire spraying was recorded the lowest in diuron + ametryne @ 5100 ml/ha with 24.75 T. portulacastrum weeds/m2, 5 P. oleracea weeds/m2 and 5.75 A. viridis; followed by diuron + ametryne @ 4000 ml/ha with 29 T. portulacastrum weeds/m2, 8 P. oleracea weeds/m2 and 10 A. viridis which were performing superior to market standards. The percentage weed control recorded in all the treatments proved that diuron + ametryne when applied at 5100 ml/ha showed maximum weed control 84.36% (T. portulacastrum), 87.88% (P. oleracea) and 88.94% (A. viridis). At 4000 ml/ha recorded 81.67% (T. portulacastrum) 80.61% (P. oleracea) and 80.77% (A. viridis) of weed control. Thereby proving that these active ingredients when applied in combination at the doses of 5100, 4000 ml/ha showed synergistic activity and weed control superior to market standard.

Table 8. Efficacy of diuron 20% + ametryne 35% (along with Zn @80ml/ha) SC formulation against Sedges (Cyperus iria):
Treatments Dose / Ha
(Gm/L) Pre-Count 7 DAS 14 DAS 28 DAS 35 DAS Average Percent Weed Control
Diuron + ametryne SC 3065 23 19 14 10 20 15.75 63.79
Diuron + ametryne SC 4000 21 17 12 8 18 13.75 68.39
Diuron + ametryne SC 5100 21 11 10 7 15 10.75 75.29
Diuron 80% SC 4000 20 15 12 12 18 14.25 67.24
Ametryne 80% WDG 2500 19 13 7 8 21 12.25 71.84
Mesotrione 2.27 + atrazine 22.7% SC 3500 21 19 13 12 22 16.5 62.07
Topramezone 10g/L + atrazine 300g/L SC 3000 19 17 12 11 25 16.25 62.64
Untreated Control -- 19 25 38 49 62 43.5
C.i – Cyperus iria
DAS-Days After Spray
The sedge weeds species were very well known for their mode of propagation to be through rhizomes and the very same character of sedges made them to be very difficult to control and took their place as one of the most noxious weeds in all the different weed species available in the field level. The sedges found in the sugarcane field in this experiment belong to a single species i.e., Cyperus iria. The population before spraying ranged between 19-23 weeds/m2. After the application of different herbicide treatments, the herbicide combination treatments diuron + ametryne when applied at 5100 gm/ha showed maximum reduction in weed population of 11 weeds/m2 at 7 days after spraying, 10 weeds/m2 at 14 days after spraying and 7 weeds/m2, 15 weeds/m2 at 28DAS, 35DAS in the weed species viz., C. iria. The average weeds population after the entire spraying was recorded as the lowest in diuron + ametryne @ 5100 ml/ha with 10.75 weeds/m2 and followed by diuron + ametryne @ 4000 ml/ha with 13.75 weeds/m2 which were performing superior to market standards. The percentage weed control recorded in all the treatments proved that diuron + ametryne when applied at 5100 ml/ha showed maximum weed control 75.29 % and at 4000 ml/ha recorded 66% of weed control. The weeds when sprayed with individual molecule separately recorded low percent weed control viz.,67.24% and 71.84% there by proving that these molecules when applied in combination at the doses of 6250, 5000 gm/ha showed synergistic activity and weeds control superior to market standard. Even the treatment diuron + ametryne when applied at 3065 ml/ha (lowest dose of the herbicide combination) also showed percent weed control of 63.79% which is also superior to the market standard.
Table 9. Phytotoxicity of diuron 20% + ametryne 35% (along with Zn @80ml/ha) SC formulation on Sugarcane crop:
Treatments DAS Visual Rating Scale
Yellowing Necrosis Wilting Vein
Clearing Leaf Tip /
Margin Dying Stunting / Dwarfing
Diuron + ametryne SC@3065 gm/Ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Diuron + ametryne SC@4000 ml/Ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Diuron + ametryne SC @5100 ml/Ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Diuron 80%SC @4000 ml/Ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Ametryne 80%WDG @2500 ml/Ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Mesotrione 2.27 + atrazine 22.7% SC @3500 ml/Ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Topramezone 10g/L + atrazine 300g/L SC @3000 ml/Ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Untreated Control 5 0 0 0 0 0 0
10 0 0 0 0 0 0
DAS-Days After Spray
The phytotoxicity effect of the herbicide combination i.e., diuron + ametryne SC on sugarcane crop was tested after 5 and 10 days after spraying. The crop was checked for symptoms like yellowing, necrosis, wilting, vein clearing, leaf tip or leaf margin dying and stunting or dwarfing of plants. After thorough observations, it could be concluded that the crop did not show any symptoms of phytotoxicity. Therefore, the present herbicide combination can be considered a safe molecule.
Table 10. Effect of diuron 20% + ametryne 35% (along with Zn @80ml/ha) SC formulation on crop quality
Treatments Dose / Ha (gm/ml) Cane Yield (T/Ha) Cane Length Cane Diameter (Cms) No. Millable Shoots-
000 / Ha Sugar Content
(% Brix)

Diuron + ametryne SC 3065 76 213 2.5 98 17.98
Diuron + ametryne SC 4000 78 218 2.5 99 18.78
Diuron + ametryne SC 5100 84 226 2.8 108 19
Diuron 80% SC 4000 73 224 2.2 94 17
Ametryne 80% WDG 2500 70 225 2.3 88 15
Mesotrione 2.27 + atrazine 22.7% SC 3500 56 195 2.2 81 14.89
Topramezone 10g/L + atrazine 300g/L SC 3000 65 216 2 78 15
Untreated Control -- 45 185 1.8 64 12
The cane yield among different herbicide treatments showed that diuron + ametryne when applied at 5100ml/ha, 4000ml/ha and 3065ml/ha recorded the highest cane yield of 84 t/ha, 78 t/ha and 76 t/ha which shows that the herbicidal combination when applied at three different doses viz., low, medium, and high have similar positive effect on the cane yield of sugarcane crop. While the same herbicides when applied alone i.e., Diuron 80 SC @ 4000ml/ha gave 73 t/ha and ametryne 80 WDG @ 2500 ml/ha gave 70 t/ha yield. The cane length and cane diameter were also recorded highest just as in the case of cane yield. The highest length of cane was in the treatment diuron + ametryne SC @5100ml/ha with 226cm long cane in followed by diuron + ametryne SC @4000ml/ha with 218cm long cane. Similar trend was followed in cane diameter with 2.8cm and 2.5cm diameter in diuron + ametryne when applied at 5100ml/ha, 4000ml/ha. The number of millable shoots (000’ ha-1) was also recorded as the highest as 108, 99 in diuron + ametryne when applied at 5100ml/ha, 4000ml/ha. The sugar content was recorded highest in diuron + ametryne when applied at 5100ml/ha, 4000ml/ha and 3065ml/ha viz., 19%, 18.78% and 17.98% respectively. It is evident based on the results the herbicide combination has a synergistic effect when applied together especially the inclusion of Zinc in the combinational mixture improved the cane quality in the current case. It has phytotonic effect on sugarcane crops apart from efficiently controlling the weed populations. Though the results of diuron + ametryne when used at the strength of 20% + 35% showed significant management of weeds and considerably good yield and cane quality. The performance of this molecule was inferior to the molecule prepared with the strength of 15% + 30% which showed very high percent weed managed, recorded higher yield with better sugar content. Therefore, it proved that diuron + ametryne (15% + 30%) + zinc (80gm/ha) SC formulation showed synergistic effect in relation to weed management and enhanced zinc micronutrient effect on plants when sprayed along with herbicide on 30 days old crop this helped in countering higher yield and good quality canes with higher sugar content.
It is to be understood that this disclosure is not limited to a particular compositions or specific constituents, which can, of course, vary and that the terminology used herein is for the purpose of describing embodiments only and is not intended to be limiting the scope of the invention. As used in this specification and the appended claims, the singular forms "a," "an," and "the" include plural referents unless the content clearly dictates otherwise, and equivalents thereof known to those skilled in the art and so forth.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this disclosure pertains. Although any methods and materials similar or equivalent to those described herein can be used in the practice for testing of the disclosure(s), specific examples of appropriate materials and methods are described herein. The examples set forth above are provided to give those of ordinarily skilled in the art a complete description of how to make and use the embodiments of the compositions or specific constituents, methods of practice, and are not intended to limit the scope of what the inventors regard as their invention. Modifications of the above-described modes for carrying out the invention that is obvious to persons skilled in the art are intended to be within the scope of the following claims. All patents and publications mentioned in the specification are indicative of the levels of skill of those skilled in the art to which the disclosure pertains.
While specific embodiments of the present invention are explicitly disclosed herein, the above specification and examples herein are illustrative and not restrictive. It will be understood that various modifications may be made without departing from the spirit and scope of the invention. Many variations of the disclosure will become apparent to those skilled in the art upon review of this specification and the embodiments below. The full scope of the invention should be determined by reference to the embodiments, along with their full scope of equivalents and the specification, along with such variations. Accordingly, other embodiments are within the scope of the following claims. ,CLAIMS:CLAIMS:
We Claim:
A synergistic herbicidal composition comprising:
at least one triazine herbicide;
at least one substituted phenyl urea herbicide;
at least one nutrient; and
at least one agriculturally acceptable excipient.
The composition as claimed in claim 1, wherein the triazine herbicide is ametryn and the substituted phenyl urea herbicide is diuron.
The composition as claimed in preceding claims, wherein ametryne and diuron are present in the weight ratio of (1-40): (1-40).
The composition as claimed in claim 1, wherein the nutrient selected from but not limited to the group comprising zinc, iron, magnesium, phosphorus, manganese, chlorine, nickel, calcium and/or potassium.
The composition as claimed in claim 1, wherein the agriculturally acceptable excipient selected from but not limited to group liquid medium, surfactant, stabilizer, anti-freezing agent, antifoaming agent, anticaking agent, dispersing agent and/or adjuvants.
The composition as claimed in preceding claims, wherein the herbicidal composition is formulated as suspension concentrates (SC).
The composition as claimed in preceding claims, wherein the herbicidal composition is formulated as wettable powder (WP).
The composition as claimed in preceding claims, wherein the herbicidal composition obtained from the preceding claims is used to control broadleaf weeds, sedges and annual grasses in rice, wheat, fruits, roots, tubers, vegetables, maize, grains, sugarcane, cereals, field crops and various other crops for general weed control.
The composition as claimed in preceding claims, wherein the herbicidal composition is applied to pre-emergence, early-post and post-emergence stages of crops by conventional methods over the target areas.