Description:Field of invention
The present invention relates to a novel insecticidal combination. The present invention particularly relates to an insecticidal composition comprising said combination that effectively controls insect pests. The present invention further relates to a process for preparing said insecticidal composition.
Background of the invention
The protection of crops and their produce from insect pest damage is essential for enhancement of agriculture produce. Even after the availability of alternative types of pest control, chemical control remains an important tool for prevention and control of pests in agriculture, especially insect pests.
Insecticides of varied types and structural groups have been reported in literature and large numbers are in commercial use. However, these chemicals must be used judiciously to protect the environment and natural ecosystems. In this regard, application of combination of agricultural pesticides has emerged as an attractive approach to ensure that the chemical load in the environment is reduced. A combination of agricultural pesticides may be applied sequentially, as a tank mix application or as a ready-to-use composition.
A tank mix of pesticides in many cases results in a heterogenous solution and suffers from several other problems like difficult handling, sedimentation, etc. Hence a pre-mix composition is much preferred. However, it is a known fact that not all actives are compatible with each other, and therefore rigorous efforts are required to develop a stable, synergistic, efficacious pesticidal composition or formulation.
The present invention aims to provide an insecticidal composition with aforesaid advantages. More importantly, the present invention aims at providing a composition wherein the insecticidal efficacy of the composition is considerably higher than the sum of the activities of the solo active compounds when applied individually and that results in effective control of insect pests in agricultural crops.
Object of the invention
It is an object to provide a novel, stable, synergistic, insecticidal composition comprising a) Flonicamid b) Spirotetramat and c) Matrine along with at least one agriculturally acceptable excipient.
It is yet another object of the present invention to provide a ready-to-use insecticidal composition that shows an enhanced bioefficacy at a reduced dosage as compared to insecticidal actives when applied individually.
It is yet another object of the present invention to provide an insecticidal composition that is effective against sucking pests including but not limited to white fly, jassids, aphids, thrips in various agricultural crops particularly, cotton.
It is yet another object of the present invention to provide an insecticidal composition that is environmentally safe and non-phytotoxic.
Summary of the invention
Accordingly, in one aspect, the present invention provides a novel, stable, synergistic, environmentally safe, cost-effective insecticidal composition comprising a) Flonicamid b) Spirotetramat and c) Matrine along with at least one agriculturally acceptable excipient.
In another aspect, the present invention provides a novel, stable, synergistic, environment friendly insecticidal composition that is effective in controlling sucking pests, particularly whitefly, jassids, aphids and thrips in agricultural crops, particularly cotton.
In yet another aspect, the present invention provides a process for preparing said insecticidal composition.
Detailed description of the invention:
Those skilled in the art will be aware that the invention described herein is subject to variations and modifications other than those specifically described. It is to be understood that the invention described herein includes all such variations and modifications. The invention also includes all such steps, features, compositions and compounds referred to or indicated in this specification, individually or collectively, and all combinations of any two or more of said steps or features.
Unless otherwise specified, all terms used in disclosing the invention, including technical and scientific terms, have the meaning as commonly understood by one of the ordinary skill in the art to which the invention belongs. For further guidance, term definitions may be included to better appreciate the teaching of the present invention.
As used herein, the term “plant” refers to any plant or part thereof including serial and subterranean parts of the plant. It is contemplated that the parts of the plant may be for example, flowers, fruits or vegetables, shoots, leaves, needles, stalks, stems, fruiting bodies, seeds also roots and that parts of the plants may or may not be attached to the remainder of the plant.
The term “locus” of a plant as used herein is intended to embrace the place on which the plants are growing, where the plant propagation materials of the plants are sown or where the plant propagation materials of the plants will be placed into the soil.
The term “plant propagation material” is understood to denote generative parts of a plant, such as seeds, vegetative material such as cuttings or tubers, roots, fruits, tubers, bulbs, rhizomes and parts of plants, germinated plants and young plants which are to be transplanted after germination or after emergence from the soil. These young plants may be protected before transplantation by a total or partial treatment by immersion.
It is to be understood that the singular forms “a,” “an,” and “the” include plural referents unless the context clearly dictates otherwise.
It should be emphasized that the term “comprises/comprising” when used in this specification is taken to specify the presence of stated features, steps or components but does not preclude the presence or addition of one or more other features, steps, components or groups thereof.
The term "insects" as used herein, includes all organisms in the class "Insecta."
The term "Insecticidal" as used herein, refers to the ability of a pesticide to increase mortality or inhibit growth rate of insects.
The terms “weight percent”, “wt-%”, “percent by weight”, “% by weight” and variations thereof, as used herein, refer to the concentration of a substance as the weight of that substance divided by the total weight of the composition and multiplied by 100. It is understood that, as used here, “percent “, “%” and the like are intended to be synonymous with “weight percent”, “wt. %”, etc..
Features that are described and/or illustrated with respect to one embodiment may be used in the same way or in a similar way in one or more other embodiments and/or in combination with or instead of the features of the other embodiments.
Flonicamid IUPAC name N-cyanomethyl-4-(trifluoromethyl)nicotinamide is a pyridine organic insecticidal compound discovered by Ishihara Sangyo Kaisha that inhibits feeding. It has a systemic and translaminar activity. Besides showing an excellent aphicidal activity it gives a long-term control over sucking insect pests such as thrips, whiteflies, planthoppers, leafhoppers, plant bugs and mealybugs in fruit trees, cereals, rice, potatoes, cotton, vegetables and ornamentals. Flonicamid has no negative impact on pollinating insects or natural enemies. Flonicamid also has a good toxicological, environmental and ecotoxicological profile.

Spirotetramat, IUPAC name [3-(2,5-dimethylphenyl)-8-methoxy-2-oxo-1-azaspiro[4.5]dec-3-en-4-yl] ethyl carbonate is a keto-enol insecticide which is the only modern 2-way systemic insecticide in the world i.e. it translocates in the xylem and phloem and thus offers “shoot to root” protection of the crop leaving pests nowhere to hide. It acts by inhibiting lipid biosynthesis, acting mainly after ingestion but also on contact, affecting eggs and larvae, with additional effects on adult fecundity. After penetrating the leaves, it is de-esterified and transported within both the xylem and phloem. It also possesses translaminar activity. It is highly effective in controlling the sucking pests including white flies, mealy bugs, root aphids and selected thrips in vegetables, cotton, soybeans and fruits.

Matrine is alkaloid extracted from dried root, plant and fruit of Sophora flavescens with organic solvent such as ethanol and is plant-derived pesticide. It has high efficiency, low toxicity, wide spectrum, systemic activity, etc. and its pesticidal mechanism is mainly contact killing and assistant stomach toxicity. The active ingredients of the pesticide have strong contact killing activity, once pests contact the pesticide, nerve centers can be paralyzed, and then the protein of the pests is solidified to block the pores of the pests so that the pests are suffocated, and meanwhile, the pesticide also has strong antifeedant effect so that the pests cannot eat the substances.
Matrine has a strong killing effect on rice leaf roller larva and eggs, and also has strong preventing and treating effect on chilo suppressalis (striped rice stem borer) and tryporyza incertulas (yellow stem borer). Matrine was procured from Shaanxi Green Agri Co.Ltd. , 3-1-302 Vanke Yungu Building ,No.31 West of Zhangba Road, High-Tech District, Xi'an City, Shaanxi Province, China 710075.
The Applicant submits that the present invention does not use any biological material originating in India. The biological pesticide (Matrine) used in the instant invention is not obtained from India. Matrine was procured from Shaanxi Green Agri Co. Ltd., 3-1-302 Vanke Yungu Building ,No.31 West of Zhangba Road, High-Tech District, Xi'an City, Shaanxi Province, China 710075.
In an embodiment, the present invention provides an insecticidal combination comprising a) Flonicamid b) Spirotetramat and c) Matrine along with at least one agriculturally acceptable excipient.
In an embodiment, the present invention provides an insecticidal composition comprising said combination that possesses an enhanced insecticidal efficacy at reduced cumulative dosage as compared to solo active ingredient compounds when applied individually.
A pesticidal composition may be applied sequentially or as tank mix or as a ready to use composition or formulation. Sequential application increases the chemical load in the soil and hence is not an environmentally friendly approach. A tank mix is an unscientific practice adopted by the farmers which usually results in a heterogenous mixture wherein the measuring and mixing of chemicals is complicated. It has applicator and inhalation hazards besides other disadvantages like sedimentation issues, higher transportation costs and are environmentally less safe.
On the other hand, a ready-to-use composition or formulation results in a homogenous solution, are easy to handle with lower chances of sedimentation, application hazard, phytotoxicity and are environmentally safer.
Thus, in an embodiment, the present invention provides a ready-to-use insecticidal composition comprising a) Flonicamid b) Spirotetramat and c) Matrine along with at least one agriculturally acceptable excipient.
The present invention thus provides an insecticidal composition comprising a) Flonicamid in an amount ranging from 10-40% w/w b) Spirotetramat in an amount ranging from 5-50% w/w and c) Matrine in an amount ranging from 0.5-2% w/w along with at least one agriculturally acceptable excipient.
In a preferred embodiment, Flonicamid is present in an amount of 18% w/w, Spirotetramat is present in an amount of 13.5 % w/w, Matrine is present in an amount of 0.2 % w/w along with at least one agriculturally acceptable excipient.
In an embodiment, the insecticidal composition of the present invention comprising said actives ingredients exhibits synergistic effect in control of sucking pests in agricultural crops, particularly whitefly, jassids, aphids, thrips in cotton crop.
In an embodiment, the insecticidal composition of the present invention may be applied to a variety of agricultural crops including but not limited to cotton, cucurbits, chili, tomato, brinjal and okra.
In an embodiment, the insecticidal composition of the present invention further comprises at least one agriculturally acceptable excipient. In an embodiment, at least one excipient is selected from the group consisting of at least an anti-freezing agent, at least a wetting-cum-dispersing agent, at least a defoamer, at least a biocide and at least a thickener.
In a more preferred embodiment, the insecticidal composition of the present invention is formulated as a suspension concentrate comprising at least an anti-freezing agent, at least a wetting-cum-dispersing agent, at least a defoamer, at least a biocide and at least a thickener.
In an embodiment, the anti-freezing agent is selected from the group comprising monoethylene glycol, diethylene glycol, polypropylene glycol, ethylene alcohols, polyethylene glycols, glycerine, epichlorohydrin, urea, glycerol, sorbitol or a mixture thereof. In a preferred embodiment, the antifreeze agent is polypropylene glycol. In an embodiment, the antifreeze agent is present in an amount of 1-15% w/w.
In an embodiment, the wetting-cum-dispersing agent is selected from the group consisting of alkyl phenol Ethoxylate, salts of alkyl naphthyl sulphonate, salts of alkyl aryl sulphonate, derivative of sulfonated fatty alcohol, blend of aromatic sulphonic acid sodium salt, Blend of alkyl aryl sulphonic acid sodium salt, sodium -N-Methyl-N-Oleyl taurate. In a preferred embodiment, the wetting-cum-dispersing agent is Alkyl phenol ethoxylate phosphate ester (available under the tradename “AVS-790”). In an embodiment, the wetting-cum-dispersing agent is present in an amount of 0.5-10%w/w.
In an embodiment, the defoamer is selected from the group consisting of silicone emulsions, long-chain alcohols, fatty acids, organic fluorine compounds and combinations thereof. In a preferred embodiment, the defoamer is silicon based. In a preferred embodiment, the defoamer is present in an amount of 0.1-10%w/w.
In an embodiment, the biocide is selected from the group comprising Clavulanic acid, Avibactum, stigmasterol and formaldehyde. In a preferred embodiment, the antibacterial agent is formaldehyde. In an embodiment, the anti-bacterial is present in an amount ranging from 0.01-5 %w/w.
In an embodiment, the thickener is selected from the group comprising polyacrylamide, polyethylene polymers, polysaccharides (long-chain 15 sugars), or vegetable oils and xanthan gum. In a preferred embodiment, the thickening agent is Xanthan gum. In an embodiment, the thickener is present in an amount ranging from 0.05-4 % w/w of the composition.
In an embodiment, the insecticidal composition is formulated in a form selected from the group comprising water-soluble concentrates (SL), emulsifiable concentrates (EC), emulsions (EW), micro-emulsions (ME), Suspension concentrates (SC), oil-based suspension concentrates (OD), flowable suspensions (FS), water-dispersible granules (WG), water-soluble granules (SG), wettable powders (WP), water soluble powders (SP), granules (GR), encapsulated granules (CG), fine granules (FG), macrogranules (GG), dry flowables (DF), aqueous suspo-emulsions (SE), capsule suspensions (CS) and microgranules (MG). In a preferred embodiment, the insecticidal composition is in the form of suspension concentrates (SC), water-dispersible granule (WDG) and wettable powder (WP).
In a preferred embodiment, the insecticidal composition of the present invention is formulated as a suspension concentrate comprising an anti-freeze agent, a wetting cum dispersing agent, a defoamer, a thickener and a biocide or antibacterial.
A suspension concentrate offers many advantages over other types of formulations. It is water-based and provides good safety and user convenience. It is suitable for many active ingredients with low water solubility. Built in adjuvants can be used for bio enhancement. Absence of dusts and flammable liquids in the formulation reduces the application hazards.
EXAMPLES:
The examples below are given solely for the purpose of illustration and are not to be construed as limitations of the present invention, as many variations thereof are possible without departing from the spirit and scope of the invention.
Example 1: Preparation of insecticidal composition as a suspension concentrate
In a preferred embodiment, the chemical composition of the present insecticidal composition is depicted below in Table 1 and coded as CI-18132 SC.

Table 1: Preferred composition of CI-18132 SC
S.no. Constituent Weight Percentage w/w
1. Flonicamid (Basis of 100%) 18.00
2. Spirotetramat (Basis of 100%) 13.5
3. Matrine (Basis of 100%) 0.2
4. Antifreeze agent (Propylene glycol) 6
5. Wetting-cum-dispersing agent 3
6. Defoamer 0.5
7. Anti-bacterial 0.2
8. Thickener 0.25
9. Demineralised water QS
Total 100

Preparation method of Suspension concentrate (SC):
Step 1: The mixture of surfactant and anti-freezing agent was diluted in demineralized water and solubilized by high-speed mixer. This was followed by addition of defoamer mix, Flonicamid technical and Spirotetramat technical followed by Matrine technical. After this, the rest of the adjuvants were added in the required weight percent to obtain a homogeneous mass.
Step 2: The mass obtained in Step 1 was subjected to grinding in sand mill. The grinding was carried out until a mean particle size of less than 5 micron was obtained.
Step 3: 2% water solution of xanthan gum (thickener with anti-bacterial in required quantity) was added under low stirring and mixed well.
Step 4: Mixing is carried out till a homogeneous mixture is obtained. Quality parameters are checked. If qualifies, proceeded for packing or transferred to storage vessel.
Example 2: Field evaluation of the bio efficacy of the present Insecticidal composition
The presently disclosed insecticidal trimix (coded as CI-18132SC) was tested for its bioefficacy against white fly and jassids on a local variety of cotton. The plants were aligned in a plot size of 50 sq. meter in a randomized block design. The trial was carried out in Hissar, Haryana in the Rabi season 2021-2022 at a temp 32? and 75% relative humidity. A total of 9 treatments were carried out in three replications. The application of the insecticidal trimix was foliar (over the top).
Measured quantity of the chemical was added to required volume of water @ 375 lit. /ha for spray. The spray tank was filled with half the quantity of clean required volume of water and then the measured chemical (according to the dose) was added followed by the rest half quantity of water. The solution was stirred well before application while ensuring thorough coverage.
Code Technical applied gm a.i./ha dose/ha
CI-18132 SC Flonicamid 18% + Spirotetramat 13.5% SC + Matrine 0.2% SC 90 + 67.5 +1 500 ml
CI-1813 SC Flonicamid 18% + Spirotetramat 13.5% SC 90 + 67.5 500 ml
CI-15102 Flonicamid 15% + Spirotetramat 10% SC + Matrine 0.2% SC 75 + 50 + 1 500 ml
CI-1510 SC Flonicamid 15% + Spirotetramat 10% SC 75 + 50 500 ml
CI-21172 Flonicamid 21% + Spirotetramat 17% SC + Matrine 0.2% SC 105 + 85 + 1 500 ml
CI-2117 SC Flonicamid 21% + Spirotetramat 17% SC 105 + 85 500 ml
CI-50 Flonicamid 50 WG (UPL) 75 150g
CI-1531 Spirotetramat 15.31 OD (BAYER) 60 400 ml
CI-1 EC Matrine 1 % EC 1 100 ml
Table 2 below provides the treatment details of the trimix in cotton crop. For purposes of convenience, the present composition has been represented by the code “CI-18132SC”.

Evaluation of bio-efficacy in cotton
For white fly and jassids
a. Count the number of live white fly and jassids from 5 leaves per 10 tagged plants before application (pre-count) and at 3 DAA (days after application), 7 DAA and 10 DAA and need basis at 5 days interval thereafter till re-infestation.
Percent control: Per-cent control of Live Population was calculated based on no. of live sucking pest population. Formula is given below.

C – T
Percent control = …………. × 100
C
Where C = No. of live population in control plot
T = No. of live population in treated plot
Table 3 (a): Bio efficacy of various mixtures of insecticides against Jassids on Cotton


Treatments gm a.i./ha Dose/ha Percent Jassids Control (Days Intervals) of both Adults & Nymphs
3 DAA % Control 7 DAA % Control 10 DAA % Control
Untreated 17 19 23
CI-18132 SC 90 + 67.5 +1 500 ml 2.2 87.06 2 89.47 3 86.96
CI-1813 SC 90 + 67.5 500 ml 3 82.35 2.3 87.89 3.5 84.78
CI-15102 75 + 50 + 1 500 ml 7 58.82 6 68.42 7 69.57
CI-1510 SC 75 + 50 500 ml 9 47.06 7.2 62.11 7.8 66.09
CI-21172 105 + 85 + 1 500 ml 3 82.35 3.4 82.11 3.5 84.78
CI-2117 SC 105 + 85 500 ml 3.4 80.00 3.5 81.58 3.9 83.04
CI-50 UPL 100 200g 4.5 73.53 4.3 77.37 5.5 76.09
CI-1531 BAYER 76.55 500ml 2.7 84.12 4.9 74.21 6.5 71.74
CI-1 EC 1 100ml 11 35.29 13 31.58 15 34.78

Table 3(b): Bio efficacy of various mixtures of insecticides against White fly on Cotton
Treatments gm a.i./ha Dose/ha Percent White fly Control (Days Intervals) Both Adults & Nymphs
3 DAA % Control 7 DAA % Control 10 DAA % Control
Untreated 32 35 42
CI-18132 SC 90 + 67.5 +1 500 ml 5 84.38 4 88.57 5 88.10
CI-1813 SC 90 + 67.5 500 ml 7 78.13 6 82.86 7 83.33
CI-15102 75 + 50 + 1 500 ml 9.5 70.31 10 71.43 13 69.05
CI-1510 SC 75 + 50 500 ml 11.5 64.06 13 62.86 15 64.29
CI-21172 105 + 85 + 1 500 ml 6.5 79.69 6 82.86 8 80.95
CI-2117 SC 105 + 85 500 ml 6.8 78.75 8 77.14 10 76.19
CI-50 (UPL) 100 200 g 8.9 72.19 10 71.43 12 71.43
CI-1531 (BAYER) 76.55 500 ml 11.2 65.00 12 65.71 15 64.29
CI-1 EC 1 100 ml 24 25.00 27 22.86 29 30.95

Results and Conclusion
Efficacy of the insecticidal composition of the present invention (CI-18132SC) was evaluated against two major pests of cotton crop ie. jassids and white fly.
As seen from Table 3(a) above, Flonicamid treatment alone at a dosage of 100 gm. ai/h shows a 77 % and 76% control of jassids at 7 and 10 days after application respectively. Spirotetramat alone at a dosage of 76.5 gm ai/ha shows 74% and 71.7% control of jassids at 7 and 10 days after application respectively. Matrine (CI-1 EC) at a dosage of 1gm ai/ha treatment alone shows a meagre 31.5% and 34.7% control of jassids at 7 and 10 days after application. Therefore, a person skilled in the art would reasonably expect to apply Flonicamid, Spirotetramat and Matrine at their recommended dosage (ie. 100+ 76.55+1 =177.55 g ai/ha) in order to get the desired effect.
However, unexpectedly and surprisingly, it was found that the trimix coded as CI-18132 SC, where the combined dosage of the active ingredients is reduced by approximately 11% ie. 158.5 g ai/ha , the percent control of jassids is 89 and 87% at 7 and 10 days after application. Thus, a higher efficacy was achieved at 11% lower dosage concentration which is unexpected and unforeseen by a person skilled in the art, in view of the general expectation of the efficacy lowering down with a lowering of the active ingredient dosage.
Further in table 3(b) above, depicting the bioefficacy of various mixtures against whitefly in cotton. Flonicamid treatment alone at a dosage of 100gai/ha shows 71.43% control of whitefly at 7 and 10 days after application. Spirotetramat alone at a dosage of 76.55 gai/ha shows a 65.7% and 64.2% control of whitefly at 7 and 10 days after application. Matrine alone at a dosage of 1g ai/ha shows a meagre 22.8% control and 31% control of whitefly at 7 and 10 days after application. Therefore, a person skilled in the art would reasonably expect to apply Flonicamid, Spirotetramat and Matrine at the recommended dosages (ie. 100+ 76.55+1 = 177.55 g ai/ha) in order to achieve the desired effect.
However, unexpectedly and surprisingly, it was found that the trimix coded as CI-18132 SC, where the c dosage of the active ingredients is reduced ie. 158.5 g ai/ha, the percent control of whitefly is 88.5% and 88.1% at 7 and 10 days after application. Thus, a higher efficacy was achieved at approximately 11% lower dosage concentration which is unexpected and unforeseen by a person skilled in the art, especially in view of the general expectation of the efficacy lowering down with a lowering of the active ingredient dosage.
Example 3: Evaluation of Phytotoxicity
The phytotoxicity of the tested composition was evaluated through visible observations. Visual observations were recorded at 3, 7 and 10 days after application of insecticidal mixtures. The parameters observed were leaf injury on tip/surface, necrosis, vein clearing, epinasty, hyponasty and wilting. For rating, the core Scale of 0 – 10 was followed for leaf injury on tips/surface as shown in table 4 below:
Table 4: Phytotoxicity symptoms Scoring and rating for leaf injury on tip/surface
Leaf injury on tips/surface Rating
0% 0
1-10% 1
11-20% 2
21-30% 3
31-40% 4
41-50% 5
51-60% 6
61-70% 7
71-80% 8
81-90% 9
91-100% 10

Table 5 (a): Evaluation of phytotoxicity symptoms at 3 days after application

Treatments gm a.i./ha Dose/ha yellowing necrosis epinasty scorching burning shortening
Untreated 0 0 0 0 0 0
CI-18132 SC 90 + 67.5 +1 500 ml 0 0 0 0 0 0
CI-1813 SC 90 + 67.5 500 ml 0 0 0 0 0 0
CI-15102 75 + 50 + 1 500 ml 0 0 0 0 0 0
CI-1510 SC 75 + 50 500 ml 0 0 0 0 0 0
CI-21172 105 + 85 + 1 500 ml 0 0 0 0 0 0
CI-2117 SC 105 + 85 500 ml 0 0 0 0 0 0
CI-50 UPL 100 200 g 0 0 0 0 0 0
CI-1531 BAYER 76.55 500 ml 0 0 0 0 0 0
CI-1 EC 1 100 ml 0 0 0 0 0 0

Treatments gm a.i./ha Dose/ha yellowing necrosis epinasty scorching burning shortening
Untreated 0 0 0 0 0 0
CI-18132 SC 90 + 67.5 +1 500 ml 0 0 0 0 0 0
CI-1813 SC 90 + 67.5 500 ml 0 0 0 0 0 0
CI-15102 75 + 50 + 1 500 ml 0 0 0 0 0 0
CI-1510 SC 75 + 50 500 ml 0 0 0 0 0 0
CI-21172 105 + 85 + 1 500 ml 0 0 0 0 0 0
CI-2117 SC 105 + 85 500 ml 0 0 0 0 0 0
CI-50 UPL 100 200g 0 0 0 0 0 0
CI-1531 BAYER 76.55 500 ml 0 0 0 0 0 0
CI-1 EC 1 100 ml 0 0 0 0 0 0
Table 5(b): Evaluation of phytotoxicity symptoms at 7 days after application
As seen in tables 5(a) & (b) above, no visible phytotoxicity symptoms were observed in cotton, indicating that the composition is crop safe.

, Claims:I/We Claim:
1. An insecticidal composition comprising:
a) Flonicamid in an amount ranging from 10-40%w/w;
b) Spirotetramat in an amount ranging from 5-50% w/w;
c) Matrine in an amount ranging from 0.5-2%w/w; and
d) at least one agriculturally acceptable excipient.
2. The insecticidal composition as claimed in claim 1, wherein said at least one agriculturally acceptable excipient is selected from the group comprising an anti-freezing agent, a wetting-cum- dispersing agent, a defoamer, a biocide and a thickener.
3. The insecticidal composition as claimed in claim 2, wherein the anti-freezing agent is present in an amount ranging from 1-15%w/w, wetting-cum-dispersing agent is present in an amount ranging from 0.5-10% w/w, defoamer is present in an amount ranging from 0.1-10% w/w, biocide is present in an amount ranging from 0.01-5% w/w and thickener is present in an amount ranging from 0.05-4% w/w.
4. The insecticidal composition as claimed in claim 2, wherein anti-freezing agent is selected from the group comprising monoethylene glycol, diethylene glycol, polypropylene glycol, ethylene alcohols, polyethylene glycols, glycerine, epichlorohydrin, urea, glycerol, sorbitol, or a mixture thereof.
5. The insecticidal composition as claimed in claim 2, wherein the wetting-cum-dispersing agent is selected from the group comprising alkyl Phenol Ethoxylate, salts of alkyl naphthyl sulphonate, salts of alkyl aryl sulphonate, derivative of sulfonated fatty alcohol, blend of aromatic sulphonic acid sodium salt, Blend of alkyl aryl sulphonic acid sodium salt and sodium -N-Methyl-N-Oleyl taurate.
6. The insecticidal composition as claimed in claim 2, wherein the defoamer is selected from the group comprising silicone emulsions, long-chain alcohols, fatty acids, organic fluorine compounds and combinations thereof.
7. The insecticidal composition as claimed in claim 2, wherein the biocide is selected from the group comprising Clavulanic acid, Avibactum, stigmasterol and formaldehyde.
8. The insecticidal composition as claimed in claim 2, wherein the thickener is selected from the group comprising polyacrylamide, polyethylene polymers, polysaccharides (long-chain 15 sugars), or vegetable oils and xanthan gum.
9. The insecticidal composition as claimed in claim 1, wherein the insecticidal composition is in a form selected from the group comprising water-soluble concentrates (SL), emulsifiable concentrates (EC), emulsions (EW), micro-emulsions (ME), Suspension concentrates (SC), oil-dispersion (OD), flowable suspensions (FS), water-dispersible granules (WG), water-soluble granules (SG), wettable powders (WP), water soluble powders (SP), dry flowables (DF), granules (GR), encapsulated granules (CG), fine granules (FG), macrogranules (GG), aqueous Suspo-emulsions (SE), capsule suspensions (CS) and microgranules (MG).
10. The insecticidal composition as claimed in claim 1, wherein the insecticidal composition is preferably in the form of a Suspension concentrate (SC).

11. The insecticidal composition as claimed in claim 1, wherein Flonicamid is present in an amount of 18%w/w, Spirotetramat is present in an amount of 13.5%w/w, Matrine is present in an amount of 0.2%w/w of said insecticidal composition along with at least one agriculturally acceptable excipient.
12. A method for controlling insect pests in agricultural crops, the method comprising contacting the insect pests with the insecticidal composition as claimed in the claims 1-11.
13. A process for preparing the insecticidal composition as claimed in claim 1-11 in the form of a suspension concentrate, the process comprising:
i. diluting the mixture of surfactant and anti-freezing agent in demineralised water and solubilizing with high-speed mixer. Adding defoamer mix, Flonicamid technical and Spirotetramat technical followed by Matrine technical.
ii. adding rest of the adjuvants in required weight percent to obtain a homogeneous mass.
iii. subjecting the mass obtained in step (ii) to grinding in sand mill until a mean particle size of less than 5 micron was obtained.
iv. adding 2% water solution of thickener and biocide to mixture under low stirring and mixing well.

14. The process as claimed in claim 13, wherein Flonicamid is present in an amount ranging from 10-40% w/w, Spirotetramat is present in an amount ranging from 5-50% w/w and matrine is present in an amount ranging from 0.5-2% w/w.
Dated This 2nd Day of November 2023

Ruchi Bhatnagar
IN/PA-3497
Agent for the applicant