DESC:Synergistic Insecticidal Composition of Dinotefuran, Pyriproxyfen and Permethrin for Controlling Insect Pests of Crop Plants
FIELD OF THE INVENTION
The present invention relates to a synergistic insecticidal or pesticidal composition comprising the combination of Dinotefuran, Pyriproxyfen and Permethrin in EC / WDG / SC / SL / OD / OS / Solid Granules and other formulations in different percentages and optionally with at least one agriculturally acceptable excipient which will facilitate in the preparation of desired formulations. The present invention also relates to the process for the preparation of insecticidal composition thereof and use of this combination for combating insecticides in and on the seeds and plants at different growth stages for crop protection and good yields.
BACKGROUND OF THE INVENTION
Crop protection is the practice of protecting the crop yields from pests, weeds, plant diseases, and other organisms that damage agricultural crops, which is critical from early stages of crop development. Preventing pests and diseases in the entire crop cycle, i.e., from root development to matured crop, leads to increased crop quality and yield. Generally, insects are very destructive to crop plants and can significantly reduce crop yields and quality. Insecticides help to minimize this damage by controlling insect pests. The use of two or more appropriate active ingredient combinations in specific dose ratios leads to synergism in crop protection.
The biggest challenge in the field of crop protection is to reduce the dosage rate of active ingredients to diminish or circumvent environmental or toxicological effects without compromising on effective crop protection against insects, in addition to long lasting and broad-spectrum protection from insects. Another challenge is to reduce the excessive application of solo chemical compounds or insecticides which invariably helps in rapid selection of insects and aid in developing natural or adapted resistance against the active compound in question.
Therefore, it is indeed necessary to use the insecticide combinations in lower doses, fast acting with the different mode of action that can provide long lasting control against broad spectrum of insects and check the resistance development in insects. The composition should have high synergistic action, no cross resistance to existing insecticidal agents, avoid excess loading of the toxicant to the environment and negligible impact to environmental safety. Thus, there is a need for synergistic insecticide compositions which could be physico-compatible formulations in the form of storage stability, safe packaging and ready to use formulations.
OBJECT OF THE INVENTION
The main objective of the present invention is to provide an insecticide mixture or combination which solves at least one of the problems as discussed above like reducing the dosage rate, broadening the spectrum of activity, or combining activity with prolonged insect control and resistance management. Thus, the combination of the present invention is designed to target and eliminate a broader spectrum of insect pests, prevent the development of resistance, and potentially reduce the risk of negative environmental impacts associated with a single insecticide.
The details of one or more embodiments of this disclosure are set forth in the accompanying description below and other features, objects, and advantages will be apparent from the description and the claims.
DESCRIPTION OF THE INVENTION
The present disclosure / specification refers to a synergistic insecticidal or pesticidal composition and the process for the preparation for crop protection.
The term “combination” can be replaced with the words “mixture” or “composition” defined or refers to as combining two or more active ingredients formulated in desired formulations.
The term “pesticide” as used in this specification refers to a substance or mixture of substances intended for preventing, destroying, repelling, or mitigating any pest or weeds which causes damage to the crop. Herbicides, insecticides, and fungicides are mainly used as pesticides which control weeds and insect pests and disease causing pathogens respectively that eventually leads to high yield of crops.
The term “insecticide” as used in this specification refers to a type of chemical compound or substance specifically designed to protect crops and kill or control insects in various agricultural, residential, and public health settings. Integrated pest management practices are encouraged, combining multiple strategies, including cultural, biological, and chemical methods, to reduce reliance on insecticides solely and promote pest control.
The term “synergism” as used in this specification refers to the interaction between two or more active compounds or other factors to produce a combined effect greater than the sum of their separate effects. The present invention involves the mixture of three active ingredients which has increased efficacy when compared to individual use and admixture of those components.
The conventional insecticides have poor activity, limited to certain insects, and are not satisfactorily maintained for prolonged periods. Even though some insecticides may bear satisfactory insecticidal effects, but they require improvements in respect of environment and health safety and are also required to achieve a high insecticidal effect at a smaller dosage and lack resistance management.
We found that this objective in part or complete can be achieved by the combination of active compounds defined at the outset. Thus, the present inventors have intensively studied to solve these problems and found that by combining insecticidal composition having dinotefuran, pyriproxyfen and permethrin in different formulation and percentages have astonishing effects in controlling insects and by reducing the amount of dosage than in a case of using an active compound alone.
Therefore, the present invention provides a novel synergistic insecticidal composition having dinotefuran, pyriproxyfen and permethrin and purpose thereof. The synergy of insecticidal composition has the main effective components of dinotefuran, pyriproxyfen and permethrin and acts by mimics the effects of acetylcholine, biomimetic of juvenile hormone and delaying the closure of voltage-gated sodium channels resulting in continuous nerve stimulation and leads to the disruption of insect metabolism, and can enable broad spectrum satisfactory insect control and protect the several crop from sucking, chewing, caterpillars, borer pests and soil insects for prolonged period of time at lower dose with no phytotoxic effect.
This combination can be developed in the form of Emulsifiable Concentrates (EC), Dispersible Concentrates (DC), Oil Dispersions (OD), Suspension Concentrates (SC), Soluble Liquids (SL), Suspoemulsion (SE), Emulsion Concentrates (EW), Microemulsions, Wettable Powders (WP), Water-Dispersible Granules (WG), Soluble Powders (SP), Granules (G), Oil Solutions (OS), Aqueous Suspensions (AS), Aqueous Solutions (AS), Microencapsulated Suspensions (ME), and Microencapsulated Emulsions (MEC), mixed formulation of Suspension Concentrate and Capsule Suspension (ZC) and other conventional formulation and with different percentages and can be used for foliar spray or soil applications.
This insecticidal combination can effectively control sucking, chewing, caterpillars, borer insects and soil insects and check the resistance development in insects in several crops. This combination is also helpful in controlling insect vectors which transmit viral diseases in plants and it can also be used in public health and household purposes for pest control. This can be a unique insecticide combination than the existing ones.
The present invention involves the mixture of three active ingredients which are classified under neonicotinoid, insect growth regulator and type I pyrethroid class of chemicals which described herein thereof.
Neonicotinoids are a broad spectrum, systemic neurotoxic insecticides that have a similar structure to nicotine. Neonicotinoids act as agonists at insect nicotinic acetylcholine receptors (nAChRs), exhibiting high selective toxicity to insects over nontarget organisms and the environment. It selectively and irreversibly binds to the postsynaptic nicotinic receptors of insects results in block sodium / potassium channels leading to blockage of transmission of nervous influx whereby affect the central nervous system to produce paralysis and death of the insect. The major neonicotinoids for pesticides are acetamiprid, clothianidin, dinotefuran, imidacloprid, thiacloprid, and thiamethoxam from which dinotefuran is preferably selected.
Dinotefuran (IUPAC name: (2-methyl-1-nitro-3-[(tetrahydro-3-furanyl) methyl)] guanidine); molecular formula: C7H14N4O3; molecular weight: 203.22 g/mol) is a broad-spectrum systemic and translaminar insecticide which belongs to neonicotinoid in the nitro-guanidine sub-class. The advantage of dinotefuran is that it does not bind to mammalian acetylcholine receptor sites, which results in its low mammalian toxicity and affects only the insects.
Dinotefuran controls the insect pests such as but not limited to aphids, whiteflies, trims, leafhoppers, leaf miners, sawflies, mole cricket, white grubs, jassids, thrips, lace bugs, billbugs, beetles, mealybugs, sawfly larvae and cockroaches on various crops and households. It controls the insects of numerous crops such as but not limited to paddy, cereals, fruits, vegetables, flowers and ornamental plants, trees, and others fruit crops such as apples, citrus fruits, grapes, and berries. Vegetable crops include tomatoes, peppers, cucumbers, and leafy greens and field crops like corn, soybeans, cotton, and wheat.
Pyriproxyfen is a pyridine-based pesticide (IUPAC name: 2-[1-Methyl-2-(4-phenoxyphenoxy)ethoxy]pyridine; molecular formula: C20H19NO3; molecular weight: 321.4 g/mol) belongs to the family of insect growth regulators (IGRs) and chitin synthesis inhibitors (CSI) which acts as a biomimetic of juvenile hormone or ecdysone hormones, two hormones that regulate insect development and provokes imbalance in the levels of this hormone in the insect, resulting in strong suppression of embryogenesis, metamorphosis and adult formation, effectively interrupting many important processes including reproduction and maturation of the insects. Pyriproxyfen affects many types of insects including but not limited to fleas, cockroaches, ticks, ants, carpet beetles, and mosquitoes which protects many crops such as but not limited to citrus, soybeans, cotton, tomatoes, beans, grapes, apples and coffee and thereof.
Type I pyrethroid are synthetic insecticides, analogues of pyrethrins class which lack cyano group. Pyrethroids target the central nervous system in insects and bind to sodium channels on nerves, causing them to remain open, resulting in repeated and extended nerve firings, which causes paralysis and death of insects.
Permethrin is a broad spectrum, non-systemic, neurotoxic synthetic pyrethroid insecticide (IUPAC name: (3-phenoxyphenyl) methyl 3-(2,2-dichloroethenyl)-2,2-dimethylcyclopropane-1-carboxylate; molecular formula: C21H20Cl2O3; molecular weight: 391.3 g/mol). Permethrin with the primary target organ being the nervous system of insects which then causes muscle spasms, paralysis and death.
The first embodiment of the present invention provides a synergistic insecticidal composition comprising:
at least one neonicotinoid class of insecticide;
at least one insect growth regulator (IGRs) and
at least one type I pyrethroid class of insecticide.
The first aspect of first embodiment, the neonicotinoid insecticide is selected from but not limited to the group comprising acetamiprid, clothianidin, dinotefuran, imidacloprid, thiacloprid, and thiamethoxam; preferably dinotefuran.
The second aspect of first embodiment, insect growth regulators (IGRs) is the group comprising Juvenile hormone analogs including but not limited to methoprene, hydroprene and pyriproxyfen; preferably pyriproxyfen.
The third aspect of first embodiment, Type I pyrethroid class of insecticide is selected from but not limited to allethrin, bifenthrin, tetramethrin, resmethrin, d-phenothrin, and permethrin; preferably permethrin.
The fourth aspect of first embodiment, synergistic insecticidal composition comprising a combination dinotefuran, pyriproxyfen and permethrin at a weight in the ratio of (1-80): (1-80): (1-80) or preferably in the ratio of (1-40): (1-40): (1-40).
The second embodiment of the present invention provides a synergistic insecticidal composition comprising:
at least one neonicotinoid class of insecticide;
at least one insect growth regulator (IGRs);
at least one type I pyrethroid class of insecticide; and
at least one agrochemical excipient.
The first aspect of second embodiment, the neonicotinoid insecticide is selected from but not limited to the group comprising acetamiprid, clothianidin, dinotefuran, imidacloprid, thiacloprid, and thiamethoxam and preferably dinotefuran.
The second aspect of second embodiment, insect growth regulator (IGRs) is selected from the group comprising Juvenile hormone analogs including but not limited to methoprene, hydroprene and pyriproxyfen and preferably pyriproxyfen.
The third aspect of second embodiment, Type I pyrethroid class of insecticide is selected from but not limited to allethrin, bifenthrin, tetramethrin, resmethrin, d-phenothrin, permethrin and preferably permethrin.
The fourth aspect of second embodiment, synergistic insecticidal composition comprising a combination dinotefuran, pyriproxyfen and permethrin at a weight in the ratio of (1-80): (1-80): (1-80); preferably in the ratio of (1-40): (1-40): (1-40) and more preferably in the ratio of (1-10): (1-20): (1-20).
The fifth aspect of second embodiment, agriculturally acceptable excipient selected from but not limited to liquid medium, surfactants, stabilizer, anti-freezing agent, antifoaming agents, anticaking agent and superior absorption agent, dispersing agents, preservatives, adjuvants and antibacterial agents.
A further aspect of second embodiment, liquid medium acts as a carrier for the active ingredient (s) and provides a stable environment for suspension.
Further aspect of second embodiment, liquid medium selected from but not limited to water, organic solvents incudes non-polar solvents like hydrocarbon solvents and cycloalkanes, ether solvents, ester solvents, ketones solvents, alcohols solvents and polar-aprotic solvents.
Further aspect of second embodiment, surfactant includes wetting agent and emulsifier.
Further aspect of second embodiment, emulsifier includes anionic emulsifiers, cationic emulsifiers, nonionic emulsifiers, amphoteric emulsifiers, phospholipids and glyceryl esters.
Further aspect of second embodiment, anionic emulsifiers selected from but not limited to sodium lauryl sulfate (SLS), sodium dodecyl benzenesulfonate (SDBS), alkyl sulfates and calcium alkyl benzene sulfonate.
A further aspect of second embodiment, cationic emulsifiers selected from but not limited to cetyl trimethyl ammonium bromide (CTAB) and stearalkonium chloride.
Further aspect of second embodiment, nonionic emulsifiers selected from but not limited to Polysorbate 80 (Tween 80), Polysorbate 20 (Tween 20), Sorbitan monolaurate (Span 20), ethoxylates, Sorbitan monooleate (Span 80) and Polyaryl sulfate esters.
Further aspect of second embodiment, amphoteric emulsifiers selected from but not limited to cocamidopropyl betaine, lauramidopropyl betaine; ethoxylated emulsifiers: ethoxylated nonylphenol (nonylphenol ethoxylate), ethoxylated sorbitan esters and ethoxylated fatty alcohols.
Further aspect of second embodiment, phospholipids is lecithin and glyceryl esters selected from but not limited to polyglycerol monooleate and glyceryl monooleate.
Further aspect of second embodiment, stabilizer includes antioxidant, chelating agent, pH adjusters, UV absorber, stabilizing polymers and inert filler.
Further aspect of second embodiment, stabilizers selected from group vegetable and seed oils selected from but not limited to soybean oil, sunflower seed oil, coconut oil, peanut oil, corn oil, castor oil, palm oil, rapeseed oil, safflower oil, olive oil, corn oil, cottonseed oil, linseed oil, tung oil and sesame oil and oxidized forms of the above oils.
Further aspect of second embodiment, adjuvant includes spreaders, stickers, penetrants, drift control agents, buffering agents, thickeners, compatibility agents, binders, biocides, pigments and safeners.
The third embodiment of the present invention provides a synergistic insecticidal composition comprising:
at least one neonicotinoid class of insecticide;
at least one insect growth regulator (IGRs);
at least one type I pyrethroid class of insecticide and
at least one agrochemical excipient.
The first aspect of third embodiment, the neonicotinoid class of insecticide is dinotefuran.
The second aspect of third embodiment, the insect growth regulator (IGRs) is pyriproxyfen.
The third aspect of third embodiment, the type I pyrethroid class of insecticide is permethrin.
The fourth aspect of third embodiment, synergistic insecticidal composition comprising a combination dinotefuran, pyriproxyfen and permethrin at a weight in the ratio of (1-80): (1-80): (1-80); preferably in the ratio of (1-40): (1-40): (1-40); and more preferably in the ratio of (1-10): (1-20): (1-20).
The fifth aspect of third embodiment, agriculturally acceptable excipient(s) incudes but not limited to liquid medium, emulsifier and stabilizer.
Further aspect of third embodiment, liquid medium selected from but not limited to water, organic solvents incudes hydrocarbon solvents and cycloalkanes, ether solvents, ester solvents, ketones solvents, alcohols solvents and polar-aprotic solvents; preferably hydrocarbon solvents and polar-aprotic solvents; and more preferably naphthalene and dimethyl formamide.
A further aspect of third embodiment, emulsifier includes anionic emulsifiers and nonionic emulsifiers.
A further aspect of third embodiment, anionic emulsifiers selected from but not limited to sodium lauryl sulfate (SLS), sodium dodecyl benzenesulfonate (SDBS), alkyl sulfates and calcium alkyl benzene sulfonate; preferably calcium alkyl benzene sulfonate.
Further aspect of third embodiment, nonionic emulsifiers selected from but not limited to Polysorbate 80 (Tween 80), Polysorbate 20 (Tween 20), Sorbitan monolaurate (Span 20), ethoxylates, sorbitan monooleate (Span 80) and polyaryl sulfate esters; preferably ethoxylates; and more preferably castor oil ethoxylates.
Further aspect of third embodiment, stabilizers selected from group vegetable and seed oils selected from but not limited to soybean oil, sunflower seed oil, coconut oil, peanut oil, corn oil, castor oil, palm oil, rapeseed oil, safflower oil, olive oil, corn oil, cottonseed oil, linseed oil, tung oil and sesame oil and oxidized forms of the above oils; preferably oxidized oils; and more preferably epoxidized soyabean oil.
The fourth embodiment of the present invention provides a synergistic insecticidal composition comprising:
dinotefuran;
pyriproxyfen;
permethrin;
liquid medium;
emulsifier and
stabilizers.
The first aspect of fourth embodiment, synergistic insecticidal composition comprising a combination dinotefuran, pyriproxyfen and permethrin at a weight in the ratio of (1-80): (1-80): (1-80); preferably in the ratio of (1-40): (1-40): (1-40); and more preferably in the ratio of (1-10): (1-20): (1-20).
Further aspect of fourth embodiment, liquid medium selected from but not limited to water, organic solvents incudes hydrocarbon solvents and cycloalkanes, ether solvents, ester solvents, ketones solvents, alcohols solvents and polar-aprotic solvents; preferably hydrocarbon solvents and polar-aprotic solvents; and more preferably naphthalene and dimethyl formamide.
A further aspect of fourth embodiment, emulsifier includes anionic emulsifiers and nonionic emulsifiers.
A further aspect of fourth embodiment, anionic emulsifiers selected from but not limited to sodium lauryl sulfate (SLS), sodium dodecyl benzenesulfonate (SDBS), alkyl sulfates and calcium alkyl benzene sulfonate, preferably calcium alkyl benzene sulfonate.
Further aspect of fourth embodiment, nonionic emulsifiers selected from but not limited to Polysorbate 80 (Tween 80), Polysorbate 20 (Tween 20), Sorbitan monolaurate (Span 20), ethoxylates, sorbitan monooleate (Span 80), and polyaryl sulfate esters; preferably ethoxylates and more preferably castor oil ethoxylates.
Further aspect of fourth embodiment, stabilizers selected from group vegetable and seed oils selected from but not limited to soybean oil, sunflower seed oil, coconut oil, peanut oil, corn oil, castor oil, palm oil, rapeseed oil, safflower oil, olive oil, corn oil, cottonseed oil, linseed oil, tung oil and sesame oil and oxidized forms of the above oils; preferably oxidized oils; and more preferably epoxidized soyabean oil.
The fifth embodiment of the present invention provides a synergistic insecticidal composition comprising:
dinotefuran;
pyriproxyfen and
permethrin.
The first aspect of fifth embodiment, synergistic insecticidal composition comprising a combination dinotefuran, pyriproxyfen and permethrin at a weight in the ratio of (1-80): (1-80): (1-80); preferably in the ratio of (1-40): (1-40): (1-40); and more preferably in the ratio of (1-10): (1-20): (1-20).
Further aspect of fifth embodiment, the composition of fifth embodiment comprising at least one agriculturally acceptable excipient there of which is / are used in preparation desired formulation.
Further aspect of the fifth embodiment, the composition of fifth embodiment is formulated as emulsifiable concentrate (EC).
The sixth embodiment of the present invention provides a synergistic insecticidal composition comprising:
dinotefuran;
pyriproxyfen;
permethrin;
naphthalene;
dimethyl formamide;
calcium alkyl benzene sulfonate;
castor oil ethoxylates and
epoxidized soyabean oil.
The first aspect of sixth embodiment, synergistic insecticidal composition comprising a combination dinotefuran, pyriproxyfen and permethrin at a weight in the ratio of (1-80): (1-80): (1-80); preferably in the ratio of (1-40): (1-40): (1-40); and more preferably in the ratio of (1-10): (1-20): (1-20).
Further aspect of sixth embodiment, the composition of sixth embodiment comprising at least one agriculturally acceptable agrochemical excipients there of which is/are used in preparation desired formulation.
A further aspect of the sixth embodiment, the composition of sixth embodiment is formulated as emulsifiable concentrate (EC).
Another embodiment of present invention provides a process for the preparation of an insecticidal formulation comprising:
add dimethyl formamide into the vessel,
add dinotefuran into the same vessel and mix well until a homogenous mixture is obtained,
add naphthalene into the above mixture and mix well,
add pyriproxyfen and permethrin into the above mixture and mix well to get clear solution,
add stabilizer and emulsifier to the above solution and mix well to get homogeneous mixture,
after mixing the above material, optionally mill or grind to get the desired particle size and remove any oversized particles or agglomerate,
then material transfer into the post blender and mix well until a homogenous mixture is obtained,
pack the formulation and seal it.
Another embodiment of the present invention, the insecticidal composition obtained from the present used to control and destroys insect pests such as but not limited to aphids, whiteflies, trims, leafhoppers, leaf miners, sawflies, mole cricket, white grubs, jassids, thrips, lace bugs, billbugs, beetles, mealybugs, sawfly larvae, fleas, cockroaches, ticks, ants, carpet beetles, and mosquitoes on several crops. It controls the insects of numerous crops such as but not limited to paddy, cereals, fruits, vegetables, flowers and ornamental plants, trees, and others. Fruit crops such as apples, citrus fruits, grapes, and berries. Vegetable crops include tomatoes, peppers, cucumbers, and leafy greens. Field crops like corn, soybeans, cotton, and wheat.
Another embodiment of the present invention, the insecticidal composition further comprises at least another agrochemical selected from a fungicide, insecticide, herbicide, biocide, nutrient, plant growth regulator, plant activator, fertilizers and likewise.
Another embodiment of the present invention, the insecticidal composition obtained from the present shows synergistic effects of better pest control with minimum resistance and resurgence and improved crop yield and quality.
Another embodiment of the present invention, the synergistic insecticidal composition can be used for prophylactic application and control the pests by applying effective amount of insecticidal composition over the target areas by conventional spraying methods, such as foliar application soil drenching etc., and avoiding excessive drift or runoff of the composition, securing thorough coverage.
Another embodiment of the present invention, synergistic insecticidal combination decreases natural hazardous effect of single active ingredient and also minimizes the residue deposition in environment.
Another embodiment of the present invention, a method of controlling pests and insects comprising synergistic insecticidal composition applying to crop in effective amount by conventional methods.
Advantages of Present invention:
The synergistic insecticidal composition of present invention can be used for prophylactic application and control the pests to increase the yield of crops compare to single component of composition, market standards and admixture of those components.
It has enhanced efficacy by combining insecticides with different modes of action, the mixture can effectively control a broader spectrum of insect pests and reduce the likelihood of resistance development and resurgence.
These insecticides may exhibit synergistic effects when combined, meaning their combined action is more potent than the sum of their individual actions. This synergy can improve the overall effectiveness of pest control, leading to better pest management outcomes.
The use of a combination of insecticides can help slow down the development of insecticide resistance in pest populations. By reducing the selection pressure on a specific mode of action, insects are less likely to develop resistance to any single insecticide in the mixture.
The synergistic insecticidal composition of the present invention will bring down pest load by targeting pests with a mixture of insecticides thereby reducing crop damage with increased yield.
The synergistic insecticidal composition of the present invention shows flexibility under different environmental conditions or against different pest species. By using a combination of insecticides, farmers and pest control operators can adapt their pest management strategies based on specific pest load and environmental factors.
The synergistic insecticidal composition of present invention has cost-effectiveness by combining insecticides with different properties may reduce the overall amount of each individual insecticide needed for effective pest control, potentially lowering the cost.
The best mode of carrying present invention is described in the below given examples. These examples are merely for illustrative purposes only, not to determine the scope of the invention and in no way limit the scope or spirit of the present invention.
EXAMPLES:
EXAMPLE 1: EMULSIFIABLE CONCENTRATE (EC) FORMULATION OF SYNERGISTIC INSECTICIDAL COMPOSITION OF THE PRESENT INVENTION
TABLE 1:
S. No Ingredient Weight / Weight %
1 Dinotefuran 4
2 Pyriproxyfen 12
3 Permethrin 12
4 Calcium Alkyl Benzene Sulfonate 2.4
5 Castor Oil Ethoxylate 9.6
6 Dimethyl Formamide 30
7 Epoxidized Soyabean Oil 3
8 Heavy Aromatic Naphthalene QS
Total 100
EXAMPLE 2: PROCESS FOR PREPARATION OF EMULSIFIABLE CONCENTRATE (EC) FORMULATION OF SYNERGISTIC INSECTICIDAL COMPOSITION OF THE PRESENT INVENTION
Dinotefuran is added to dimethyl formamide into the vessel and mixed well until a homogenous mixture is obtained. Naphthalene was added to mixture and mixed well, pyriproxyfen and permethrin were added mixture and mixed well to get clear solution. stabilizer and emulsifier were added to the above solution and mixed well to get homogeneous mixture. After mixing the above material, optionally mill or grind to get the desired particle size and remove any oversized particles or agglomerate, then material transfer into the post blender and mix well until a homogenous mixture is obtained.
EXAMPLE 3: BIO EFFICACY AND PHYTOTOXICITY TESTS OF THE PRESENT INVENTION
Presently to evaluate the efficacy of dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC formulation against sucking pests on different crops and to test their phytotoxicity on the crop after two sprayings have been conducted. Whitefly in brinjal and jassids in cotton crop are taken for evaluation. Along with dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC as a combination formulation, both the insecticides are tested individually, and the market standards selected were diafenthiuron 50% WP and flonicamid 50% WG. The tests are conducted at three dose levels viz., low, medium, and high along with the sole molecule as individual treatments and their efficiency comparison is done with the current competitive market standards. The active ingredients are tested at three different formulation strengths i.e., dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC(@625ml/ha) SC formulation, dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC(@750ml/ha) SC formulation and dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC(@875ml/ha) SC formulation. To justify the results, the overall effect and other parameters are calculated over untreated check and to see their effect on crop, its yield is recorded. The crops are first divided into plots for each treatment and replicated three times following randomized block design. The spraying method followed was foliar application with the help of a knapsack sprayer and two sprays are done when the pests reach their ETL. The interval between the two sprays is 15 days.
Method of Observations:
No. of insects (adult / nymph) / plant: select 5 random plants in the plot and count the number of insects per plant are recorded.
The observations were taken at 1 day before spraying and at 1 day, 4 days, 7 days, and 10 days after spraying. The average number of insects were also calculated at the end of each spray (two sprays). The percentage reduction in pest population is calculated.
Take the observation on the crop safety of the insecticide i.e., phytotoxicity / softener observation of insecticide after application at 5 and 10 days after application.
Parameters of Observations:
The insect pests controlled in each treatment are counted as number of adults / nymphs per plant and then calculated as percent reduction in population over untreated or controlled plot. The percent reduction is calculated by the following formula:
% reduction = (No.of insects in control plot-No.of insects in treated plot )/(No.of insects in control plot)× 100
The effect of these insecticides in combination and alone when applied on crops is assessed based on the yield (quintal per hectare). This parameter defines the crop quality.
Results:
The insecticide combination of dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC is effective against wide range of insect pests, so the different insect pests controlled in different crops in the field experiments were enlisted below,
Cotton – Jassid (Amrasca bigutulla bigutulla)
Brinjal – Whitefly (Bemesia tabaci)
Example – 3.1: Cotton - Jassid

Table 1. Efficacy of first spray application of dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC formulation against Jassids in Cotton.
Treatments Dose
(g or ml / ha) No. of adult / nymphs per plant (days after spraying) % reduction in jassids population (days after spraying)
Pre 1 4 7 10 AVG 1 4 7 10 AVG
Dinotefuran 4% + pyriproxyfen12% + permethrin 12% EC 625 14.6 5 3 9 13 8.92 68.35 83.33 59.09 53.57 66.09
Dinotefuran 4% + pyriproxyfen12% + permethrin 12% EC 750 14.2 4 3 9 10 8.04 74.68 83.33 59.09 64.29 70.35
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC 875 15 5 2 7 9 7.60 68.35 88.89 68.18 67.86 73.32
Dinotefuran 20% SG 150 14.4 9 5 10 14 10.48 43.04 72.22 54.55 50.00 54.95
pyriproxyfen 10 EC 500 15 9 7 9 13 10.60 43.04 61.11 59.09 53.57 54.20
Permethrin 25% EC 400 16 9 9 10 12 11.20 43.04 50.00 54.55 57.14 51.18
Diafenthiuron 50% WP 600 15.4 7 7 9 11 9.88 55.70 61.11 59.09 60.71 59.15
Flonicamid 50% WG 150 14.8 7 8 9 12.4 10.24 55.70 55.56 59.09 55.71 56.51
Untreated -- 15.2 15.8 18 22 28 19.80 0.00 0.00 0.00 0.00 0.00
The jassids population before initiating the spraying was recorded in between 14.2-16 jassids per plant in cotton. After the first application of different insecticide treatments, the insecticide combination treatments dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC when applied at 875 ml/ha and 750 ml/ha showed maximum reduction in jassids population of 4-5 jassids / plant at 1 days after spraying, 3-2 jassids/plant at 4 days after spraying and 7-9 jassids / plant at 7 days after spraying and 9-10 jassids / plant at 10 days after spraying in both treatments. The average jassids population after the entire spraying was recorded to be lowest in dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 875 ml/ha with 7.6 jassids / plant followed by dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 750 ml/ha with 8.04 jassids / plant which were performing superior to market standards. The percent reduction in jassids population recorded in all the treatments proved that dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC when applied at 875ml/ha was showing maximum reduction in jassids population as 73.32% and at 750 ml/ha recorded 70.35% there by proving that these molecules when applied in combination at the doses of 875, 750 ml/ha showed synergistic activity and jassids population control was superior to market standard. Even the treatment dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC when applied at 625 ml/ha (lowest dose of the insecticide combination) also showed a percent reduction of 66.09% which was also superior to the market standard. Though the first spray application of combination insecticides showed significant reduction in jassids population in cotton. The percent reduction was only 70% hence there are chances that the pest population might increase later and to prevent that second spray application the insecticidal combination was carried out.
Table 2. Efficacy of second spray application of dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC formulation against Jassid in Cotton.
Treatments Dose
(g or ml / ha) No. of adult / nymphs per plant (Days after spraying) % reduction in jassids population (Days after spraying)
Pre 1 4 7 10 AVG 1 4 7 10 AVG
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC 625 13 5 0 0 2 4.00 82.76 100 100 95.12 94.47
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC 750 10 4 0 0 0 2.80 86.21 100 100 100 96.55
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC 875 9 2 0 0 0 2.20 93.10 100 100 100 98.28
Dinotefuran 20% SG 150 14 9 3.6 3.7 4.5 6.96 68.97 88.75 89.43 89.02 84.04
Pyriproxyfen 10% EC 500 13 7.3 2 2 2.7 5.40 74.83 93.75 94.29 93.41 89.07
Permethrin 25% EC 400 12 13.3 10 8.9 10.6 10.96 54.14 68.75 74.57 74.15 67.90
Diafenthiuron 50% WP 600 11 6 5 7 11 8.00 79.31 84.38 80.00 73.17 79.21
Flonicamid 50% WG 150 12.4 8 9 12 15 11.28 72.41 71.88 65.71 63.41 68.35
Untreated -- 28 29 32 35 41 33.00 0.00 0.00 0.00 0.00 0.00
The jassids population before initiating the second spray was recorded in between 9-14 jassids per plant in cotton. This population is nothing, but the jassids population recorded at the end of the first spraying. After the second application of different insecticide treatments, the insecticide combination treatments dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC when applied at 625 ml/ha, 750 ml/ha and 875 ml/ha showed maximum reduction in jassids population by recording 5, 4, 2 jassids / plant at 1 days after spraying and 0 jassids / plant at 4 and 7 days after spraying in the three combination treatments. At, 10 days after spraying the jassids population remained 0 in dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC when applied at 875 ml/ha, 750 ml/ha and was 2 jassids / plant when applied at 625 ml/ha. The average jassids population after the entire spraying was recorded to be lowest in dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 875 ml/ha with 2.2 jassids / plant followed by dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 750 ml/ha with 2.8 jassids / plant which were performing superior to market standards. The percent reduction in jassids population recorded in all the treatments proved that dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC when applied at 875ml/ha was showing maximum reduction in jassids population as 98.28% followed by dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 750 ml/ha with 96.55% reduction and at 625 ml/ha recorded 94.47% there by proving that these molecules when applied in combination at the doses of 875, 750 and 625 ml/ha showed synergistic activity and jassids population control was superior to market standard. The insecticide molecules applied in combination when applied solely showed lesser control of jassids like when dinotefuran 20% SG @ 150g/ha recorded on 84.04% reduction in the larval population at the end of two sprays while pyriproxyfen 10% EC recorded 89.07% reduction and permethrin 25% EC recorded 67.90% reduction in jassids population. Though they are significantly controlling the jassids population the same molecules when applied in combination with lesser strength recorded higher control in jassids population proving combination insecticide is more reliable.
Table 3. Effect of dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC formulation on yield in Cotton
Treatments Dose
(g or ml / ha) Yield
(q / ha)
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC 625 10
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC 750 11.6
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC 875 12
Dinotefuran 20% SG 150 9
pyriproxyfen 10% EC 500 9.4
Permethrin 25% EC 400 8
Diafenthiuron 50% WP 600 8.6
Flonicamid 50% WG 150 8.1
Untreated -- 5
The yield of cotton recorded in different treatments as shown in the table above (Table 3.) implies that the combination molecule at the three doses positively affected the yield of the crop. The highest yield was recorded in dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 875 ml/ha with 12q/ha, followed by dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 750 ml/ha with 11.6 q/ha and dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 625 ml/ha with 10 q/ha. While the individual molecules and market standards recorded yields ranging between 8-9.4 q/ha which were inferior to the yield recorded in the combination molecule treatments.
Table 4. Phytotoxicity of dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC formulation on Cotton
Treatments Days Visual Rating Scale
Yellowing Necrosis Wilting Vein
Clearing Leaf Tip / Margin Dying Stunting / Dwarfing
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 875ml/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 750ml/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 625ml/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Dinotefuran 20% SG @ 150g/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Pyriproxyfen 10% EC @ 500ml/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Permethrin 25% EC @ 400ml/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Diafenthiuron 50% WP @ 600g/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Flonicamid 50% WG @ 150g/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Untreated 5 0 0 0 0 0 0
10 0 0 0 0 0 0
The phytotoxicity effect of the insecticide combination i.e., dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC on cotton crop was tested after 5 and 10 days after spraying. The crop was checked for symptoms like yellowing, necrosis, wilting, vein clearing, leaf tip or leaf margin dying and stunting or dwarfing of plants. After thorough observations, it could be concluded that the crop did not show any symptoms of phytotoxicity. Therefore, the present insecticide combination can be considered a safe molecular composition.
Example -3.2: Brinjal - whitefly
Table 5. Efficacy of first spray application of dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC formulation against whitefly in brinjal.
Treatments Dose
(g or ml/ha) No. of adult / nymphs per plant (Days after spraying) % reduction in whitefly population
(Days after spraying)
Pre 1 4 7 10 AVG 1 4 7 10 AVG
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC 625 21.2 11 5 6 8 10.24 67.46 86.70 84.58 80.00 79.68
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC 750 17.7 7 3 4 5 7.34 79.29 92.02 89.72 87.50 87.13
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC 875 20.5 4 1 2 3 6.10 88.17 97.34 94.86 92.50 93.22
Dinotefuran 20% SG 150 19.8 7 10.2 14 17 13.60 79.29 72.87 64.01 57.50 68.42
Pyriproxyfen 10% EC 500 22 12 5 10 14 12.60 64.50 86.70 74.29 65.00 72.62
Permethrin 25% EC 400 19.7 7 10 12.9 15.6 13.04 79.29 73.40 66.84 61.00 70.13
Diafenthiuron 50% WP 600 20 6.9 10.2 13.6 15.4 13.22 79.59 72.87 65.04 61.50 69.75
Flonicamid 50% WG 150 16.1 12 9 12 16 13.02 64.50 76.06 69.15 60.00 67.43
Untreated -- 32.6 33.8 37.6 38.9 40 36.58 0.00 0.00 0.00 0.00 0.00
The whitefly population before initiating the spraying was recorded in between 16.1-22 whiteflies per plant in cotton. After the first application of different insecticide treatments, the insecticide combination treatments dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC when applied at 875 ml/ha and 750 ml/ha showed maximum reduction in whitefly population of 4 and 7 whiteflies/plant at 1 days after spraying, 1 and 3 whiteflies/plant at 4 days after spraying and 2 and 4 whiteflies / plant at 7 days after spraying and 3 and 5 whiteflies / plant at 10 Days after spraying in both treatments. The average whiteflies population after the entire spraying was recorded to be lowest in dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 875 ml/ha with 6.10 whiteflies / plant followed by dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 750 ml/ha with 7.34 whiteflies / plant which were performing superior to market standards. The percent reduction in whitefly population recorded in all the treatments proved that dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC when applied at 875ml/ha was showing maximum reduction in whitefly population as 93.22% and at 750 ml/ha recorded 87.13% there by proving that these molecules when applied in combination at the doses of 875, 750 ml/ha showed synergistic activity and whitefly population control was superior to market standard. Even the treatment dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC when applied at 625 ml/ha (lowest dose of the insecticide combination) also showed a percent reduction of 79.68% which was also superior to the market standard. Though the first spray application of combination insecticides showed significant reduction in whitefly population in brinjal.
Table 6. Efficacy of second spray application of dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC formulation against whitefly in brinjal.
Treatments Dose
(g or ml / ha) No. of adult / nymphs per plant (days after spraying) % reduction in whitefly population (days after spraying)
Pre 1 4 7 10 AVG 1 4 7 10 AVG
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC 625 8 6 0 2 4 3 85.71 100 96.08 92.86 93.66
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC 750 5 4 0 1 2 1.75 90.48 100 98.04 96.43 96.24
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC 875 3 0 0 0 1 0.25 100 100 100 98.21 99.55
Dinotefuran 20% SG 150 17 5 13.9 16.8 19.8 13.88 88.10 69.11 67.06 64.64 72.23
Pyriproxyfen 10% EC 500 14 12 5.00 7 9 8.25 71.43 88.89 86.27 83.93 82.63
Permethrin 25% EC 400 15.6 9.90 13 16.2 19.7 14.70 76.43 71.11 68.24 64.82 70.15
Diafenthiuron 50% WP 600 15.4 10 13 16.7 18 14.43 76.19 71.11 67.25 67.86 70.60
Flonicamid 50% WG 150 16 10 9.70 14 18 12.93 76.19 78.44 72.55 67.86 73.76
Untreated -- 40 42 45 51 56 48.50 0.00 0.00 0.00 0.00 0.00
The whitefly population before initiating the second spray was recorded in between 3-17 whiteflies per plant in brinjal. This population is nothing, but the whitefly population recorded at the end of the first spraying. After the second application of different insecticide treatments, the insecticide combination treatments dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC when applied at 625 ml/ha, 750 ml/ha and 875 ml/ha showed maximum reduction in whitefly population by recording 0, 4, 6 whiteflies / plant at 1 days after spraying and 0 whiteflies / plant at 4 and 0, 1, 2 whiteflies / plant at 7 days after spraying. At, 10 days after spraying the whitefly population remained 1, 2, 4 whiteflies / plant in the three combination treatments. The average whitefly population after the entire spraying was recorded to be lowest in dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 875 ml/ha with 0.25 whiteflies / plant followed by dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 750 ml/ha with 1.75 whiteflies / plant which were performing superior to market standards. The percent reduction in whitefly population recorded in all the treatments proved that dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC when applied at 875 ml/ha was showing maximum reduction in whitefly population as 99.55% followed by dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 750 ml/ha with 96.24% reduction and at 625 ml/ha recorded 93.66% there by proving that these molecules when applied in combination at the doses of 875, 750 and 625 ml/ha showed synergistic activity and whitefly population control was superior to market standard. The insecticide molecules applied in combination when applied solely showed lesser control of whitefly like when dinotefuran 20% SG @ 150g/ha recorded on 72.23% reduction in the whitefly population at the end of two sprays while pyriproxyfen 10% EC recorded 82.63% reduction and permethrin 25% EC recorded 70.15% reduction in whitefly population. Though they are significantly controlling the whitefly population the same molecules when applied in combination with lesser strength recorded higher control in whitefly population proving combination insecticide is more reliable.

Table 7. Effect of dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC formulation on yield in Brinjal.
Treatments Dose
(g or ml / ha) Yield
(q / ha)
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC 625 237
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC 750 245
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC 875 260
Dinotefuran 20% SG 150 222
Pyriproxyfen 10% EC 500 231
Permethrin 25% EC 400 229
Diafenthiuron 50% WP 600 216
Flonicamid 50% WG 150 211
Untreated -- 191
The fruit yield of brinjal recorded in different treatments as shown in the table above (Table 7.) implies that the combination molecule at the three doses positively affected the yield of the crop. The highest yield was recorded in dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 875 ml/ha with 260q/ha, followed by dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC @ 750 ml/ha with 245q/ha and dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC @ 625 ml/ha with 237q/ha. While the individual molecules and market standards recorded yields ranging between 211-231q/ha which were inferior to the yield recorded in the combination molecule treatments

Table 8. Phytotoxicity of dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC formulation on Brinjal crop.
Treatments Days Visual Rating Scale
Yellowing Necrosis Wilting Vein
Clearing Leaf Tip / Margin Dying Stunting / Dwarfing
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 875ml/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 750ml/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC@ 625ml/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Dinotefuran 20% SG @ 150g/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Pyriproxyfen 10% EC @ 500ml/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Permethrin 25% EC @ 400ml/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Diafenthiuron 50% WP @ 600g/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Flonicamid 50% WG @ 150g/ha 5 0 0 0 0 0 0
10 0 0 0 0 0 0
Untreated 5 0 0 0 0 0 0
10 0 0 0 0 0 0
The phytotoxicity effect of the insecticide combination i.e., dinotefuran 4% + pyriproxyfen 12% + permethrin 12% EC on brinjal crop was tested after 5 and 10 days after spraying. The crop was checked for symptoms like yellowing, necrosis, wilting, vein clearing, leaf tip or leaf margin dying and stunting or dwarfing of plants. After thorough observations, it could be concluded that the crop did not show any symptoms of phytotoxicity. Therefore, the present insecticide combination can be considered a safe molecular composition.
It is to be understood that this disclosure is not limited to a particular compositions or specific constituents, which can, of course, vary and that the terminology used herein is for the purpose of describing embodiments only and is not intended to be limiting the scope of the invention. As used in this specification and the appended claims, the singular forms "a," "an," and "the" include plural referents unless the content clearly dictates otherwise, and equivalents thereof known to those skilled in the art and so forth.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this disclosure pertains. Although any methods and materials similar or equivalent to those described herein can be used in the practice for testing of the disclosure(s), specific examples of appropriate materials and methods are described herein. The examples set forth above are provided to give those of ordinarily skilled in the art a complete description of how to make and use the embodiments of the particular compositions or specific constituents, methods of practice, and are not intended to limit the scope of what the inventors regard as their invention. Modifications of the above-described modes for carrying out the invention that is obvious to persons skilled in the art are intended to be within the scope of the following claims. All patents and publications mentioned in the specification are indicative of the levels of skill of those skilled in the art to which the disclosure pertains.
While specific embodiments of the present invention are explicitly disclosed herein, the above specification and examples herein are illustrative and not restrictive. It will be understood that various modifications may be made without departing from the spirit and scope of the invention. Many variations of the disclosure will become apparent to those skilled in the art upon review of this specification and the embodiments below. The full scope of the invention should be determined by reference to the embodiments, along with their full scope of equivalents and the specification, along with such variations. Accordingly, other embodiments are within the scope of the following claims.
,CLAIMS:CLAIMS:
We Claim:
1. A synergistic insecticidal composition comprising:
(a) at least one Neonicotinoid class of insecticide;
(b) at least one insect growth regulator (IGRs);
(c) at least one type I pyrethroid class of insecticide; and
(d) at least one agrochemical excipient.
2. The composition as claimed in claim 1, the neonicotinoid insecticide is dinotefuran, the insect growth regulator (IGRs) insecticide is pyriproxyfen and type I pyrethroid class of insecticide is permethrin.
3. The composition as claimed in preceding claims, wherein dinotefuran, pyriproxyfen and permethrin are present in the weight in the ratio of (1-80): (1-80): (1-80).
4. The composition as claimed in claim 1, wherein the agrochemical excipient is / are selected from the group comprising liquid medium, emulsifier, and stabilizer.
5. The composition as claimed in claim 4, wherein the emulsifier is selected from anionic emulsifier and nonionic emulsifier.
6. The composition as claimed in preceding claims, the insecticidal composition obtained from the present used to control and destroys insects as well as other small pests (such as mites or nematodes) aphids, whiteflies, thrips, leafhoppers, leafminers, sawflies, mole cricket, white grubs, lacebugs, billbugs, beetles, mealybugs, and cockroaches are all common problems and can be controlled with any low-toxicity insecticide sold specifically for houseplants.
7. The composition as claimed in claim 1, the synergistic insecticidal composition is formulated as emulsifiable concentrate (EC).
8. The composition as claimed in preceding claims, the synergistic insecticidal composition is used for prophylactic application and control the pest in various crops.