DESC:FORM 2
THE PATENTS ACT, 1970
(39 OF 1970)
&
The Patents Rules, 2003
COMPLETE SPECIFICATION
(See section 10; rule 13)
1. TITLE OF THE INVENTION – SYNERGISTIC INSECTICIDAL FORMULATION OF PROPARGITE AND LAMBDA-CYHALOTHRIN
2. APPLICANT(S)
a) NAME: MEGHMANI ORGANICS LIMITED
b) NATIONALITY: INDIAN
c) ADDRESS: MEGHMANI HOUSE, BEHIND SAFAL PROFITAIRE, CORPORATE ROAD, PRAHLADNAGAR, AHMEDABAD, GUJARAT, INDIA-380015

3. PREAMBLE TO THE DESCRIPTION
COMPLETE SPECIFICATION
The following specification describes the invention and the manner in which it is to be performed

SYNERGISTIC INSECTICIDAL FORMULATION OF PROPARGITE AND LAMBDA-CYHALOTHRIN

FIELD OF THE INVENTION:

The present invention relates to a synergistic insecticidal formulation of Propargite and Lambda-cyhalothrin. The present invention particularly relates to a synergistic insecticidal formulation of Propargite and Lambda-cyhalothrin, wherein the said composition is Suspoemulsion (SE) formulation and the process of preparation thereof.

BACKGROUND OF THE INVENTION:

Pesticides are the chemicals which are applied on any infected surface of plants to kill or eliminate fungus, bacteria, insects, snails, slugs, or weeds. Pesticides are broadly used in agriculture and have facilitated a significant increase in yield of crops and productivity.

In order to control the harmful effects of insects on crops, an extensive range of active insecticide compounds have been developed. Many active insecticide compounds in different formulations are commercially available. The efficacy of these conventional insecticide compounds is restricted in controlling certain groups of insect. Thus, the problem of controlling a broad spectrum of insects by a single product still exists. Presently, several insecticidal formulations are used in comparatively higher concentrations and doses. This leads to a higher cost of the product, higher residues on plant produce and also inside the soil causing toxicity to non-target organisms and have deleterious effects on the desirable insect species and other soil dwelling organisms.

Many researchers have developed a variety of chemicals and their formulations which are effective in controlling the insects from destroying the crops. Nevertheless, there is a continuous requirement for novel compositions and new methods to control the broad range of insects without harming the ecosystem and water bodies. Specifically, in view to achieve economical results and eco-friendly compositions, many efforts have been made to maximize the performance of known active ingredients. Also, there is a need for insecticidal compositions which demonstrate synergistic effect at lower dose and cost.

Propargite (2-(4-tert-butylphenoxy) cyclohexyl prop-2-ynyl sulfite) is a synthetic insecticide that belongs to the class of ‘sulfite ester acaricide’. Propargite was first introduced as an acaricide by Uniroyal Chemical in 1994. It is often supplied as emulsifiable concentrates, oil in water emulsion, water-soluble bags or wettable powder.

Propargite is a sulfite ester of acaricide for control of many species of phytophagous mites (particularly motile stages) on a variety of crops, including fruit trees, nuts, tomatoes, vegetables, ornamental crops, cotton, maize, peanuts and sorghum.

Propargite was first disclosed in patent US 3272854 and chemically represented as below structure:

The molecular formula of Propargite is C19H26O4S and molecular weight is 350.47 g/mol and having water solubility of 0.215 mg/L at 20oC. Propargite is the insecticide from IRAC (Insecticide Resistance Action Committee) Group 12C class. It is a complex molecule with three chiral features. Propargite is a non-systemic acaricide with predominantly contact action, and long residual activity.

Lambda-cyhalothrin is a synthetic pyrethroid insecticide. It has lower aqueous solubility, is relatively volatile and is non-mobile. Based on its chemical properties, lambda-cyhalothrin has low risk of leaching into ground water. Lambda-cyhalothrin is listed in the 3A group according to the Insecticide Resistance Action Committee (IRAC) classification.

The IUPAC name of Lambda-cyhalothrin is (R)-a-cyano-3-phenoxybenzyl (1S)-cis-3-[(Z)-2-chloro-3,3,3–trifluoropropenyl]- 2, 2- dimethyl cyclopropane carboxylate and (S)-a-cyano-3-phenoxybenzyl (1R)-cis-3-[(Z)-2-chloro-3, 3, 3 - trifluoropropenyl] -2, 2-dimethylcyclopropanecarboxylate and the molecular formula is C23H19ClF3NO3. The relative Molecular mass of Lambda-cyhalothrin is 449.85 g / mol. The solubility of Lambda-cyhalothrin in water is 0.005 mg/l at 20ºC.

Lambda-cyhalothrin is chemically represented as below structure:

Lambda-cyhalothrin is used to control a wide spectrum of insect pests, e.g. aphids, beetles, thrips, Lepidoptera larvae, Coleoptera larvae and adults, etc. Lambda-cyhalothrin is used to provide protection from pests in cereals, ornamentals, potatoes, vegetables, cotton and other crops. It also provides good control of insect-borne plant viruses and is also used for controlling insect pests in public health.

Lambda-cyhalothrin is a non-systemic insecticide with contact and stomach action and repellent properties. It gives rapid knockdown and long residual activity. Lambda-cyhalothrin is commercially marketed as encapsulated suspension, emulsion concentrate, oil in water emulsion, Ultra-low volume formulation, water dispersible granule or wettable powder.

In India or anywhere in the world, in agricultural production, using synthetic or chemical agent is the most effective means in order to control pests. However, the main disadvantage of such insecticide is that on long run, the insects develop resistance towards that insecticide and as a result to produce effective result, higher concentration of the insecticide is to be used. Here again using higher dose of insecticide may result in some detrimental effect to soil as well as environment. Therefore, in general practice, usually combination of insecticide with another insecticide or another fungicide is used in different concentration as per requirement.

However, when combination of one or more insecticide are used, the main challenge comes is to develop a formulation or composition which is stable, easy to use, safe and cost effective with superior efficacy. The present invention satisfies the existing needs, as well as others, and generally overcomes the deficiencies found in the prior art.

Chinese patent CN105580834A describes a multifunctional combined insecticide, which is characterized in that 30-40 grams of methomyl, 20-30 grams of Propargite and 10-20 grams of pyrethroid preparation are uniformly mixed in each kilogram of water; wherein the pyrethrin preparation is selected from one of fenpropathrin, cypermethrin, deltamethrin or cyfluthrin. This prior art does describe the combination of Propargite with pyrethroid but nowhere discloses combination of Propargite and Lambda-Cyhalothrin in SE formulation.

Japanese patent JPH05112411 relates to an insecticidal composition for agriculture and horticulture, which comprises at least one synthetic pyrethroid insecticidal compound and Propargite as active ingredients. This prior art does describe the combination of Propargite with pyrethroid but nowhere discloses combination of Propargite and Lambda-Cyhalothrin in SE formulation.

Indian patent 202021035156 relates to an insecticidal composition comprising: a) Lambda-cyhalothrin in an amount of 0.1% to 50% by weight of the composition; b) at least one another insecticide in an amount of 0.1% to 50% by weight of the composition; and c) a mixture of a weak acid and its salt. This prior art nowhere discloses the SE formulation of Propargite and Lambda-Cyhalothrin.

Indian patent 235/MUM/2015 relates to a composition comprising Bifenthrin and Propargite in a Suspoemulsion form. The composition further comprises at least one surfactant, at least one stabilizer, at least one viscosity modifying agent, at least one anti-freezing agent, at least one carrier and at least one anti-foaming agent. This prior art does describe the combination of Propargite with pyrethroid but nowhere discloses the combination of Propargite and Lambda-cyhalothrin in SE formulation.

Combinations of different pesticides have been practiced in the art, but the physical stability of such combination has been a concern. Therefore, there is a need for a synergistic insecticidal composition which is compatible and stable over long periods.

However, there is no formulation or composition available of Propargite and Lambda-cyhalothrin in Suspoemulsion formulation which is stable and yet effective.

In the present invention, inventors have arrived to a synergistic insecticidal formulation of Propargite and Lambda-cyhalothrin in Suspoemulsion formulation and the process of preparation which is stable, effective and overcome all the problems mentioned in prior art as described herein.

OBJECT OF THE INVENTION

The principal object of the present invention is to provide a synergistic insecticidal formulation of Propargite and Lambda-cyhalothrin.

Another object of the present invention is to provide a synergistic insecticidal formulation of Propargite and Lambda-cyhalothrin, wherein the said composition is in Suspoemulsion (SE) formulation.

Yet another object of the present invention is to provide a synergistic insecticidal formulation of Propargite and Lambda-cyhalothrin, wherein the said composition is in Suspoemulsion (SE) formulation, which is effective against a broad spectrum of pests, mites and insects.

Another object of the present invention is to provide a process to manufacture a synergistic Suspoemulsion formulation of Propargite and Lambda-cyhalothrin.

Yet another object of the present invention is to provide a synergistic insecticidal Suspoemulsion formulation of Propargite and Lambda-cyhalothrin which is stable, improved and ready to use.

Another object of the present invention is to provide a synergistic insecticidal Suspoemulsion formulation of Propargite and Lambda-cyhalothrin which is easy to manufacture.

Another object of the present invention is to provide a synergistic insecticidal Suspoemulsion formulation of Propargite and Lambda-cyhalothrin which is effective and farmer friendly.

Yet another object of the present invention is to provide a synergistic insecticidal formulation of Propargite and Lambda-cyhalothrin, wherein the said composition is in Suspoemulsion formulation which provides enhanced insecticidal effect on insects and mites which in turn results in better yield of crops.

SUMMARY OF THE INVENTION
The present invention discloses a synergistic insecticidal formulation of Propargite and Lambda-cyhalothrin, wherein the said composition is in Suspoemulsion (SE) formulation comprising: Propargite technical in the range of 47.5-52.5% w/w, lambda-cyhalothrin technical in the range of 4.75-5.5% w/w, co-solvent in the range of 9-12% w/w, surfactant in the range of 0.5-5% w/w, dispersing agent in the range of 0.5-8% w/w, fillers in the range of 0.05-5% w/w and adjuvants. The present invention more particularly discloses a synergistic insecticidal formulation of Propargite and lambda-cyhalothrin and process of preparation thereof which is easy to manufacture, effective on broad spectrum of pests, lower the rate of application of the active ingredients, enhanced insecticidal effect on pests, farmer friendly, stable, ready-to-use and cost effective.

DETAILED DESCRIPTION OF THE INVENTION

The detailed description set forth below is intended as a description of exemplary embodiments and is not intended to represent the only forms in which the exemplary embodiments may be constructed and/or utilized. The description sets forth the functions and the sequence of steps for constructing and/or operating the exemplary embodiments. However, it is to be understood that the same or equivalent functions and sequences which may be accomplished by different exemplary methods are also intended to be encompassed within the spirit and scope of the invention.

As defined herein, all scientific and technical terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the invention belongs. Although any process and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, the preferred methods and materials are now described.

Unless specifically defined otherwise, all technical and scientific terms used herein shall be taken to have the same meaning as commonly understood by one of ordinary skill in the art. Unless the context requires otherwise, throughout the specification which follow, the word “comprise” and variations thereof, such as, “comprises” and “comprising” are to be construed in an open, inclusive sense that is as “including”, but not limited to.

As used in the description herein and throughout the claims that follow, the meaning of “a”, “an” and “the” includes plural reference unless the context clearly dictates otherwise. In some embodiments, the numerical parameters set forth in the written description are approximations that can vary depending upon the desired properties sought to be obtained by a particular embodiment.

As used herein, the terms "insecticide effect" and "insecticide activity" mean any direct or indirect action on the target insect that results in reduced feeding damage on the seeds, roots, shoots and foliage of plants as a result of foliar spray of said formulation.

As used herein, the term "pest" means any insect, rodent and microbes targeted for treatment with a formulation of the invention. Target pest means any insect, rodent and microbes that are harmful for the plants and decrease the productivity of plants.

The present invention is all about providing a synergistic insecticidal formulation of Propargite and Lambda-cyhalothrin, wherein the said composition is in Suspoemulsion formulation and process of preparation thereof.

As per one embodiment, the present invention provides a synergistic insecticidal Suspoemulsion formulation comprising Propargite and Lambda-cyhalothrin with the following mass percentage of the composition:
Sr. No. Raw Material Concentration range (% w/w)
1 Propargite Technical (Purity 95% w/w) 47.5-52.5
2 Lambda-cyhalothrin Technical (Purity 96% w/w) 4.75-5.5
3 Co-solvent 9-12
4 Dispersing agent 0.5-8
5 Surfactant 0.5-5
6 Fillers and Adjuvants 0.05-5

Active ingredient is the actual chemical in the product mixture that controls the pests, insects and microbes. Active ingredient plays a key role in the functioning of the product mixture.

As per one embodiment, the active ingredient for the present invention is Propargite and Lambda-cyhalothrin, wherein the concentration range of Propargite is in the range of 47.5-52.5% w/w and Lambda-cyhalothrin is in the range of 4.75-5.5% w/w in SE formulation.

As per one embodiment, a co-solvent is added to a primary solvent in small amounts to increase the solubility of a poorly-soluble compound. The concentration of co-solvent in the present invention is in the range of 9-12% w/w, wherein the co-solvent is Octylphenol Ethoxylate 1.5 mol.

A dispersing agent is a substance which adsorbs onto the surface of particles and helps to preserve the state of dispersion of the particles and prevents them from re-aggregating. Dispersing agents are added to agrochemical formulations to facilitate dispersion and suspension during manufacture, and to ensure the particles re-disperse into vehicle or carrier.

As per one embodiment, the concentration range of dispersing agent in the insecticidal composition of the present invention is in the range of 0.5-8% w/w. The dispersing agent in the present invention is selected from a group of anionic tristyryl phenol sulphate, polyaryl phenyl anionic ether sulfate, acrylic comb copolymer dispersant, tristyryl phenol ethoxylate or a combination thereof.

Wetting agents/ surfactants are chemical agents which lower the surface tension of liquid by a considerable amount in order to allow it to spread upon the penetrating surface. It is well within the competence of those skilled in the art to determine whether a particular surface active agent (surfactant) is a wetting agent or not.

As per one embodiment, the concentration range of surfactant in the insecticidal combination of the present invention is in the range of 0.5-5% w/w. The surfactant for present invention is selected from group consisting of tristyryl phenol ethoxylate, polycarboxylate sodium salt, sodium lauryl sulphate, polydimethyl siloxane or combination thereof.

An adjuvant is an additive which is added to make the formulations more effective, that improves performance, safety, storage, or handling of an active ingredient.

As per one embodiment, the insecticide formulation also contains one or more additional adjuvants selected from filler, anti-freezing agent, polysaccharides, anti-foaming agent, anti-microbial agent, thickening agent, biocides and vehicle in the concentration range of 0.05-5% w/w.

The anti-freezing agent in the present invention is selected from monoethylene glycol, ethylene glycol, propylene glycol, glycerin, urea or a combination thereof.
The filler in the present invention is selected from lactose monohydrate, hydrated aluminum silicate crystalline mineral or a combination thereof.

The anti-foaming agent is selected from the group consisting of, but not limited to, polydimethyl siloxane emulsion and polydimethyl siloxane powder.

In one embodiment of the present invention, the composition comprises Propargite, Lambda-cyhalothrin, at least one co-solvent, at least one dispersing agent, at least one wetting agent, at least one anti-freezing agent, at least one anti-foaming agent, at least one filler, at least one anti-cracking agent, at least one biocide and at least one thickening agent.

In yet another embodiment of the present invention, the benefits of the synergistic insecticidal SE formulation of Propargite and Lambda-cyhalothrin and its process of preparation are described as follows:
1. Easy to manufacture.
2. Effective on wide range of insects and broadening the spectrum of insecticidal activity to other pests.
3. Reduction in the rate of application of the active ingredients.
4. Adequate control of the pests with the aid of the compositions according to the invention, even at a rate of application at which the individual compounds are totally ineffective.
5. Product is adapted to use for practice by farmers.
6. Cost effective
7. Stable
8. Less toxic in comparison to solid granular formulations

In yet another embodiment of the present invention there is provided a process for preparation of Propargite and Lambda-cyhalothrin Suspoemulsion (SE) formulation.

The following examples are meant to illustrate the present invention. The examples are presented to exemplify the invention and are not to be considered as limiting the scope of invention.

EXAMPLE
Example 1: Insecticidal Suspoemulsion (SE) composition of the present invention.
Propargite 50% + Lambda-cyhalothrin 5% Suspo Emulsion (SE)
Sr No. Chemical Composition Percent (% w/w) Function
1 Propargite Technical (Purity-95.00%) 50.50 Active ingredient
2 Lambda-cyhalothrin Technical (Purity-96.00%) 5.30 Active ingredient
3 Octylphenol Ethoxylate 1.5 mol 10.0 Co-solvent
4 Anionic tristyrylphenol sulfate 1.1 Dispersing cum Suspending agent
5 Polyarylphenyl anionic ether sulfate 1.5 Dispersing Agent
6 Acrylic comb copolymer dispersant 0.5 Dispersing agent
7 Mono ethylene Glycol 2.5 Anti-freezing agent
8 Tristyrylphenol Ethoxylate 2.5 Wetting cum Dispersing agent
9 Polydimethyl siloxane emulsion 0.1 Anti-foaming agent
10 Hydrated aluminum silicate crystalline mineral 0.5 Filler
11 Silicon Dioxide 0.5 Anti-cracking agent
12 Polysaccharide 0.26 Thickening agent
13 1,2-Benzisothiazolin-3-One 0.05 Biocide
14 DM Water 24.69 Carrier
Total 100.00

Example 2: Stability study of Example 1
2.1: Heat stability for 14 days at 54 degrees centigrade & cold stability 7 days at 0 degrees centigrade
Parameters Specification (in house) Initial Heat stability at 54±2 degrees centigrade Cold stability at 0±2 degrees centigrade
Propargite content percent by mass 47.5 to 52.5 50.56 50.3 50.41
Lambda-cyhalothrin content percent by mass 4.75-5.5 5.48 5.22 5.33
Propargite suspensibility percent mini. 80 92.99 92.01 91.22
Lambda-cyhalothrin suspensibility percent mini. 80 95.20 94.80 93.71
Wet sieve test (Passing through 75 mic) ( % m/m) 99% Min 99.71 99.65 99.58
pH range (1% aqueous suspension) 6 to 8 7.23 7.41 7.61
Pourability (Rinse residue) 95% min. 98.41 98.18 98.01
Specific gravity 1.08- 1.18 1.139 1.131 1.128
Viscosity at spindle no. 62,20 rpm 500 to 800 600 670 710
Particle size (micron) D90<10 5.41 5.89 6.1
Persistent foam ml (after 1 minute) max. 60 40 43 50
Spontaneity of dispersion (% m/m) 90% min 95.5 95.8 95.2
Emulsion stability (in 342 ppm hard water for 1 hour) No creaming at the top and sedimentation on bottom Complies Complies Complies

2.2: Room Temperature storage stability up to 12 months

Parameters Specification (in house) 1 month 6 month 12 month
Propargite content percent by mass 47.5 to 52.5 50.52 50.32 50.22
Lambda-cyhalothrin content percent by mass 4.75-5.5 5.46 5.38 5.3
Propargite suspensibility percent mini. 80 92.5 90.66 88.2
Lambda-cyhalothrin suspensibility percent mini. 80 95.1 92.6 91.87
Wet sieve test (Passing through 75 mic) (% m/m) 99% Min 99.7 99.61 99.41
pH range (1% aqueous suspension) 6 to 8 7.10 7.51 7.68
Pourability (Rinse residue) 95% min. 98.76 98.52 98.25
Specific gravity 1.08- 1.18 1.142 1.138 1.139
Viscosity at spindle no. 6220 rpm 500 to 800 610 638 640
Particle size (micron) D90<10 2.1, 5.22 2.8, 5.66 3.0, 6.5
Persistent foam ml (after 1 minute) max. 60 43 48 55
Spontaneity of dispersion (% m/m) 90% min 95.5 95.8 95.2
Emulsion stability (in 342 ppm hard water for 1 hour) No creaming at the top and sedimentation on bottom Complies Complies Complies

Example 3: Field Trial Studies
Field trial experiments were conducted to evaluate the efficacy of Propargite 50% + Lambda-cyhalothrin 5% SE formulation in comparison to its solo applications. The observations were recorded by counting the no. of pests before the treatment, 7 days after of treatment and after 15 days after treatment on the crops. The percent population reduction/control of pests was calculated by below formula:
% Population reduction/ Control = (Mean population in Untreated plot) - (Mean population in Treated Plot) X 100
(Mean population in Untreated Plot)

Example 3(a): Efficacy of Propargite 50% + Lambda-cyhalothrin 5% SE formulation on Chilly Fruit Borer (Helicoverpa armigera) population control.
Location: Bellary (Kurugod) Karnataka, India
Season: Kharif-2021
Crop: Chilly
Date of sowing: 09/06/2021
Date of application: 19/08/2021
Treatment details Dose
(g/ml) /ha Pre
treatment 7 DAT* 15 DAT Mean Percent Reduction/ Control
T1 Propargite 50% + Lambda-cyhalothrin 5% SE 750 ml/ha 11.00 8.00 5.00 6.50 45.26
T2 Propargite 50% + Lambda-cyhalothrin 5% SE 1000 ml/ha 10.00 7.00 2.75 4.88 58.95
T3 Propargite 50% + Lambda-cyhalothrin 5% SE 1250 ml/ha 10.00 6.75 2.50 4.63 61.05
T4 Propargite 57% EC 1500 ml/ha 10.75 7.50 5.75 6.63 44.21
T5 Lambda-Cyhalothrin 5.0 % EC 600 g/ha 10.25 7.25 6.50 6.88 42.11
T6 Untreated control 10.50 11.25 12.50 11.88
CV** (%) 13.36 9.26 17.19
CD*** 5% 2.42 1.28 1.72
Note: *DAT: Days After Treatment, **CV= Co-efficient of Variation, *** CD= Critical Difference

The results of the above experiment show that the Propargite 50% + Lambda-cyhalothrin 5% SE formulation show synergistic effect in terms of % Population reduction/ Control of Fruit Borer in Chilly. Maximum synergism is obtained at the dosage application rate of 1250ml/ha with 61.05% population reduction/ control of Fruit Borer in Chilly.

Example 3(b): Efficacy of Propargite 50% + Lambda-cyhalothrin 5% SE formulation on Chilly Mites (Polyphagotarsonemus latus) population control.
Location: Bellary (Kurugod) Karnataka, India
Season: Kharif-2021
Crop: Chilly
Date of sowing: 09/06/2021
Date of application: 19/08/2021
Treatment details Dose
(g/ml) /ha Pre
treatment 7 DAT* 15 DAT Mean Percent Reduction/ Control
T1 Propargite 50% + Lambda-cyhalothrin 5% SE 750 ml/ha 114.75 99.50 98.75 99.13 24.19
T2 Propargite 50% + Lambda-cyhalothrin 5% SE 1000 ml/ha 118.50 96.50 60.75 78.63 39.87
T3 Propargite 50% + Lambda-cyhalothrin 5% SE 1250 ml/ha 118.00 88.50 47.75 68.13 47.90
T4 Propargite 57% EC 1500 ml/ha 114.25 108.75 89.00 98.88 24.38
T5 Lambda-Cyhalothrin 5.0 % EC 600 g/ha 113.75 102.75 106.50 104.63 19.98
T6 Untreated control 113.75 132.50 124.00 130.75
CV** (%) 3.28 4.75 13.22
CD*** 5% 6.60 8.51 18.37
Note: *DAT: Days After Treatment, **CV= Co-efficient of Variation, *** CD= Critical Difference

The results of the above experiment show that the Propargite 50% + Lambda-cyhalothrin 5% SE formulation show synergistic effect in terms of % Population reduction/ Control of Mites in Chilly. Maximum synergism is obtained at the dosage application rate of 1250ml/ha with 47.90% population reduction/ control of Mites in Chilly.

Example 3(c): Efficacy of Propargite 50% + Lambda-cyhalothrin 5% SE formulation on Brinjal Fruit Borer (Leucinodes orbonalis) population control.
Location: Kolar, Karnataka, India
Season: Kharif-2021
Crop: Brinjal
Date of sowing: 28/05/2021
Date of application: 29/08/2021
Treatment details Dose
(g/ml) /ha Pre
treatment 7 DAT* 15 DAT Mean Percent Reduction/ Control
T1 Propargite 50% + Lambda-cyhalothrin 5% SE 750 ml/ha 9.75 6.50 5.50 6.00 50.52
T2 Propargite 50% + Lambda-cyhalothrin 5% SE 1000 ml/ha 9.00 5.25 3.50 4.38 63.92
T3 Propargite 50% + Lambda-cyhalothrin 5% SE 1250 ml/ha 9.00 5.50 3.00 4.25 64.95
T4 Propargite 57% EC 1500 ml/ha 9.75 7.25 5.75 6.50 46.39
T5 Lambda-Cyhalothrin 5.0 % EC 600 g/ha 9.25 7.25 6.25 6.75 44.33
T6 Untreated control 9.50 12.00 12.25 12.13
CV** (%) 14.59 12.80 22.76
CD*** 5% 2.38 1.62 2.41
Note: *DAT: Days After Treatment, **CV= Co-efficient of Variation, *** CD= Critical Difference

The results of the above experiment show that the Propargite 50% + Lambda-cyhalothrin 5% SE formulation show synergistic effect in terms of % Population reduction/ Control of Fruit Borer in Brinjal. Maximum synergism is obtained at the dosage application rate of 1250ml/ha with 64.95% population reduction/ control of Fruit Borer in Chilly.

Example 3(d): Efficacy of Propargite 50% + Lambda-cyhalothrin 5% SE formulation on Brinjal Red Spider Mites (Tetranychus urticae) population control.
Location: Kolar, Karnataka, India
Season: Kharif-2021
Crop: Brinjal
Date of sowing: 28/05/2021
Date of application: 15/07/2021
Treatment details Dose
(g/ml) /ha Pre
treatment 7 DAT* 15 DAT Mean Percent Reduction/ Control
T1 Propargite 50% + Lambda-cyhalothrin 5% SE 750 ml/ha 150.75 112.50 84.50 98.50 40.80
T2 Propargite 50% + Lambda-cyhalothrin 5% SE 1000 ml/ha 154.5 100.25 65.50 82.88 50.19
T3 Propargite 50% + Lambda-cyhalothrin 5% SE 1250 ml/ha 154 92.25 50.75 71.50 57.02
T4 Propargite 57% EC 1500 ml/ha 150.25 116.75 89.00 102.88 38.17
T5 Lambda-Cyhalothrin 5.0 % EC 600 g/ha 149.75 117.25 98.00 107.63 35.31
T6 Untreated control 152.25 161.50 171.25 166.38
CV** (%) 2.75 5.60 11.16
CD*** 5% 7.26 11.38 18.10
Note: *DAT: Days After Treatment, **CV= Co-efficient of Variation, *** CD= Critical Difference

The results of the above experiment show that the Propargite 50% + Lambda-cyhalothrin 5% SE formulation show synergistic effect in terms of % Population reduction/ Control of Red Spider Mites in Brinjal. Maximum synergism is obtained at the dosage application rate of 1250 ml/ha with 57.02% population reduction/ control of Red Spider Mites in Brinjal.

Technical advancements:
The present disclosure described herein above has several technical advantages including, but not limited to, the realization of:
• A synergistic insecticidal Suspo- Emulsion formulation of Propargite 50% + Lambda-cyhalothrin 5%;
• A synergistic insecticidal Propargite 50% + Lambda-cyhalothrin 5% SE formulation which is in stable form;

From the results in Examples 1-3, the pesticidal combination of Propargite 50% + Lambda-cyhalothrin 5% SE formulation claimed in the present invention has a synergistic effect on control of pests.

Throughout this specification, the word “comprise”, or variations such as “comprises” or “comprising”, will be understood to imply the inclusion of a stated element, integer or step, or group of elements, integers or steps, but not exclusion of any other element, integer or step, or group of elements, integers or steps.

Although the foregoing invention has been described in some way by way of illustration and example of purposes of clarity of understanding, it will be readily apparent to those of ordinary skill in the art in light of the teachings of this invention that certain changes and modifications may be made thereto.

Dated this 22nd day of August, 2023

Signature:
Name: Patel Ankit (CEO)
,CLAIMS:We Claim:
1. A synergistic insecticidal formulation consisting of:
a) Propargite Technical (Purity-95.00%) in the concentration range of 47.5-52.5% w/w,
b) Lambda-cyhalothrin Technical (Purity-96.00%) in the concentration range of 4.75-5.5% w/w,
c) Co-solvent in the concentration range of 9-12% w/w
d) One or more Dispersing agent in the concentration range of 0.5-8% w/w,
e) One or more Surfactant in the concentration range of 0.5-5% w/w,
f) One or more Fillers and Adjuvants in the concentration range of 0.05-5% w/w.

2. The synergistic insecticidal formulation as claimed in claim 1, wherein the formulation is Suspo- Emulsion (SE) formulation.

3. The synergistic insecticidal formulation as claimed in claim 1, wherein Propargite Technical (Purity-95.00%) is in the concentration range of 47.5-52.5% w/w.

4. The synergistic insecticidal formulation as claimed in claim 1, wherein Lambda-cyhalothrin Technical (Purity-96.00%) is in the concentration range of 4.75-5.5% w/w.

5. The synergistic insecticidal formulation as claimed in claim 1, wherein the co-solvent is Octylphenol Ethoxylate 1.5 mol in the concentration range of 9-12%w/w.

6. The synergistic insecticidal formulation as claimed in claim 1, wherein the dispersing agent is selected from a group of anionic tristyryl phenol sulphate, polyaryl phenyl anionic ether sulfate, acrylic comb copolymer dispersant, tristyryl phenol ethoxylate or a combination thereof in the concentration range of 0.5-8% w/w.

7. The synergistic insecticidal formulation as claimed in claim 1, wherein the surfactant is selected from group consisting of tristyryl phenol ethoxylate, polycarboxylate sodium salt, sodium lauryl sulphate, polydimethyl siloxane or combination thereof in the concentration range of 0.5-5% w/w.

8. The synergistic insecticidal formulation as claimed in claim 1, wherein the adjuvants are fillers, anti-freezing agent, anti-foaming agent, anti-cracking agent, thickening agent, biocide and carrier in the concentration of 0.05-5% w/w.

9. The synergistic insecticidal formulation as claimed in claim 8, wherein the filler is Hydrated Aluminum Silicate crystalline mineral, the Anti-foaming agent is Polydimethyl siloxane emulsion, the anti-freezing agent is Mono ethylene glycol, the anti-cracking agent is Silicon Dioxide, the Thickening agent is Polysaccharide, the Biocide is 1,2- Benzisothiazolin-3-one and the carrier is DM water.

10. A synergistic insecticidal formulation of Propargite and Lambda-cyhalothrin, consisting of:
a) 50.50% w/w of Propargite Technical,
b) 5.30% w/w of Lambda-cyhalothrin Technical,
c) 10.00% w/w of Octylphenol Ethoxylate 1.5 mol,
d) 1.10% w/w Anionic tristyrylphenol sulfate,
e) 1.50% w/w of Polyarylphenyl anionic ether sulfate
f) 0.50% w/w of Acrylic comb copolymer dispersant,
g) 2.50% w/w of Mono Ethylene Glycol,
h) 2.50% w/w of Tristyryl Ethoxylate,
i) 0.10% w/w of Polydimethyl siloxane emulsion,
j) 0.50% w/w of Hydrated aluminum silicate crystalline mineral,
k) 0.50% w/w of Silicone dioxide,
l) 0.26% w/w of Polysaccharide,
m) 0.05% w/w of 1,2-Benzisothiazolin-3-one.

Dated this 22nd of August, 2023
Signature:
Name: Patel Ankit (CEO)
Meghmani Organics Limited