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Synthesis Of Nanoparticle Of Calcium Oxide Using Plant Extract Through Dmso (Dimethyl Sulfoxide) Solvent

Abstract: Synthesis of nanoparticle of Calcium oxide using plant extract through DMSO (Dimethyl Sulfoxide) solvent is a method of synthesizing calcium oxide nanoparticles, comprising the steps of providing a solution of calcium ions & providing plant extract solution and combining the calcium ion solution and the plant extract solution while stirring at a temperature of 40° C to produce the calcium oxide nanoparticles. In where Calcium nitrate tetra hydrate (Ca(N03)2.4H20 ) used as a calcium ion source, DMSO used as a solvent and plant extract used as a reducing agent, Incubation period results in a change of color of the mixture to Light Brown which is a visual confirmation of the synthesized NPs . Further, synthesized nanoparticles are further characterized using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM).Fig 1

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Patent Information

Application #
Filing Date
27 February 2019
Publication Number
10/2019
Publication Type
INA
Invention Field
CHEMICAL
Status
Email
Parent Application
Patent Number
Legal Status
Grant Date
2021-03-15
Renewal Date

Applicants

BIOFAC INPUTS PRIVATE LIMITED
Unit-I : Plot No.74C, Anrich Industrial Estate IDA Bollaram, Hyderabad

Inventors

1. MR. LAXMI NARAYAN REDDY
Unit 1, Plot No. 74c, Anrich Industrial Estate IDA Bollaram, HYDERABAD, 502325

Specification

In June 1999, Richard Smalley, Nobel laureate in chemistry, addressed the US House Committee on Science on the benefits of nanotechnology. "The impact of nanotechnology on the health, wealth, and lives of people," he said, "will be at least the equivalent of the combined influences of microelectronics, medical imaging, computer-aided engineering and man-made polymers developed in this century."
Calcium oxide (CaO), commonly known as quicklime or burnt lime, is a widely used chemical compound. It is a white, caustic, alkaline, crystalline solid at room temperature. Nanoparticles that occur naturally such as volcanic ash, soot from forest fires or the incidental byproducts of combustion processes are physically and chemically heterogeneous and often termed as ultrafine particles.
Recent advances in the field of nanotechnology, particularly die ability to prepare highly ordered nanoparticulates of any size and shape, have led to the development of new biocidal agents. Nano-materials are called "a wonder of modern medicine". It is stated that antibiotics kill perhaps a half dozen different disease-causing organisms but nano-materials can kill plentiful.
Metal nanoparticles have various functions that are not observed in bulk phase and have been studied extensively because of their exclusive catalytic, optical, electronic, magnetic and antimicrobial wound healing and anti-inflammatory properties . Over the past few decades, inorganic nanoparticles, whose structures exhibit significantly novel and improved physical, chemical, and biological properties and functionality due to their nano-scale size, have elicited much interest.

Biosynthesis of nanoparticles is an approach of synthesizing nanoparticles using plants having biomedical applications. This approach is an environment-friendly, cost-effective, biocompatible, safe, green approach Green synthesis includes synthesis through plants, bacteria, fungi, algae etc. NPs synthesized from biomimetic approach show more catalytic activity and limit the use of expensive and toxic chemicals. These plant extract secrete some phytochemicals that act as both reducing agent and capping or stabilization agent.
The "green" synthetic strategy requires the capping materials to be environmentally benign, non-toxic and renewable. In this context, plant extract coating seems to be a promising route to accomplish this task, based on its reliability, high protecting performance, good stability, biocompatibility and ease of implementation.
Plants are most preferred source of NPs synthesis because they lead to large-scale production and production of stable, varied in shape and size NPs . Bio-reduction involves reducing metal ions or metal oxides to 0 valence metal NPs with the help of phytochemicals like polysaccharides, polyphenols compounds, vitamins, amino acids, alkaloids, terpenoids secreted from the plant.
BACKGROUND OF THE INVENTION
Calcium carbonate is a widely available natural inorganic compound, also known as limestone, chalk or marble. It is a white, caustic, alkaline, crystalline solid at room temperature.
Calcium Rich Food for Birds. All birds need plenty of calcium in their diets, as it facilitates bone formation and normal nerve, brain and muscle function. However, laying females need an especially high amount to form their eggs, the shells of which are almost pure calcium carbonate.

In relation to poultry production, lime is a generic term used to refer only to the manufactured forms of lime. These forms of lime are quicklime, which is calcium oxide (CaO), and hydrated lime, which is calcium hydroxide (Ca(0H)2), The terms whitewash and, less commonly, milk of lime are used to refer to a suspension of hydrated lime in water. (For the purposes of this article, the term lime does not include agricultural lime, which is crushed limestone. Agricultural lime should not be used in poultry houses.)
PRIOR ART
Patent application number 201818038901
The present invention describes novel nanoparticle compositions, and systems and methods utilizing them for treating disorders and/or conditions. Methods generally involve administering nanoparticle compositions (e.g., nanoparticle compositions comprising at least one known therapeutic agent and/or independently active biologically active agent; and/or empty nanoparticle compositions) to a subject in need thereof.
Patent application number 201741005011
A process of preparing nano zero valent iron particles (nZVI) - calcium alginate beads for the removal dyes present in an effluent is provided. The nZVI is obtained by mixing 0.1 M Ferrous suphate (FeS04) and Ethylene di-amine tetra acetic acid, EDTA. The nZVI thus formed is immobilized in calcium alginate beads and made into a size of 2-3 mm. The nZVI -calcium alginate beads are added to the effluent and allowed to contact with the dye present in the effluent. The dye is bound to the surface and trapped inside the nZVI - calcium alginate beads are removed from the effluent to obtain a dye free effluent. The percentage of removal of dye from the effluent is increased when the initial concentration of nZVI and the contact time is increased.
Patent application number 201611009447
The present invention provides a nano phosphatic hybrid geopolymeric corrosion resistant coating material. The tailored precursor of corrosion resistant coating material is obtained by a process involving, together dry grinding of raw materials fly ash, sodium hydroxide, rice

husk, tri calcium phosphate and cetyl dimethyl ammonium bromide optionally with sodium silicate, in solid powder form. The developed coating material obtained by adding water to tailored precursor contains nano sized phosphatic compounds of Cancrisilite (sodium aluminium carbonate silicate hydrate), quartz, mullite, heamatite, sodium aluminium silicate, Herschelite (sodium aluminium silicate hydrate), Sucrose, a D-Glucose, Native cellulose, and phenol, responsible for providing improved corrosion resistant properties and adhesion to the mild steel substrates. The geo-polymeric coating material is used as an anti- corrosive, heat resistant coating material on various materials e.g. mild steel substrates.
Patent application number 2765/CHE/2015
The present invention relates to composition of Soil Based Mineral amendments and more particularly of Nano calcium silicate (NCS) which is to be used as a soil amendment in small concentrations and preparation thereof.
Patent application number 620/DEL/2009
The process of making nano-sized calcium hydroxy apatite powder and its composite with P-Ca3 (P04)2 (3-TCP) and other calcium phosphates through modified solution combustion synthesis using pure or mixed fuels has been disclosed. The phase pure nano-sized calcium hydroxy apatite and the composites comprised of a mixture of calcium hydroxy apatite, B-tri-calcium phosphate and other phosphates. In accordance with this invention the resultant product obtained by modified solution combustion synthesis is fluffy foam like mass composed of isometric spherical particles of 20-150 nm size. The nano-sized calcium hydroxy apatite powder and its composite material can also be processed to yield a porous bioactive scaffold and bone filler materials.
Patent application number 7504/DELNP/2006
Synthetic nano-sized crystalline calcium phosphate, particularly hydroxyapatite, having a specific surface area in the range of 150 m2/g to 300 m2/g, is described. The nano-sized crystalline calcium phosphate may be in the form of a powder or in the form of a coating on a surface. A method of producing a nano-sized crystalline calcium phosphate powder or coating is also described. The method comprises formation of a liquid crystalline phase in a water solution of calcium, phosphor and a surfactant, placing the phase in an ammonia atmosphere so that nano-sized crystajp^p^rmed, followed by^either rempva^of the^u/factant with a

solvent and recovering the nano-sized crystals to obtain the powder, or diluting the ammonia-treated liquid crystalline phase with a hydrophobic organic solvent to create a microemulsion of the nano-sized crystals in water, dipping an oxide layer-coated surface of an object into the microemulsion, or alternatively saving the step of ammonia treatment of the liquid crystalline phase until after the dipping of the surface of an object into the microemulsion, followed by removal of the organic solvent and the surfactant from the surface to obtain the coating.
Patent application number WO2009011520A2
The present invention relates to nano-particles containing calcium which show an excellent absorption rate compared with conventional calcium formulations, have high purity and uniform nano-structure, and can be produced easily in large scale without using expensive devices- and a method for preparing the same.
Patent application number US8057682B2
The present invention relates to methods of making and using and compositions of metal nanoparticles formed by green chemistry synthetic techniques. For example, the present invention relates to metal nanoparticles formed with solutions of plant extracts and use of these metal nanoparticles in removing contaminants from soil and groundwater and other contaminated sites. In some embodiments, the invention comprises methods of making and using compositions of metal nanoparticles formed using green chemistry techniques.
Patent application number KR101S18971B1
The invention Chitosan - relates to ionized calcium combinations and a method of manufacturing the same, and more particularly, after preparing a mixture solution by dissolving the chitosan and calcium nitrate, and dried to ehitosan-relates to a process for preparing the ionized calcium combinations will be. Chitosan according to the invention the manufacturing method of ionized calcium combinations are removed the toxins can be safe for a human body and improves the absorption of calcium and ketones acid, it can be effectively used as a composite dietary supplements and nutritional crops.
Patent application number US20130288887A1
The present invention discloses a process for producing a nano calcium carbonate slurry from
a feedstock ofwaste gypsum, wherein: an aqueous gypsum slurry of the feedstock is mixed

with ammonia water by stirring; with C02 injected in the slurry is under continuous stirring until the calcium sulfate in the waste gypsum is completely carbonated into nano calcium carbonate; after filtration, the filter cake is dispersed in water to obtain the nano calcium carbonate slurry. This process is easy to operate and to obtain a low-cost and a lower decomposition temperature of calcium carbonate. The present invention also discloses a nano calcium carbonate slurry and its application in preparation of a CaO-based carbon dioxide adsorbent and complex catalyst used for a reactive sorption enhanced reforming process for hydrogen production from methane. The CaO-based carbon dioxide adsorbent prepared shows good cycle stability and fast sorption rate, and complex catalyst used for reactive sorption enhanced methane steam reforming can obtain the hydrogen with purity of more than 90%.
Patent application number CN101961638A
The invention discloses a method for preparing a wear-resistant nano calcium oxide-based carbon dioxide (C02) reaction adsorbent. The method comprises the following steps of: dispersing calcium carbonate (CaC03) particles in water; adding aluminasol; stirring until pulp is well distributed; spraying and pelleting to obtain microspherical particles with particle diameter of 20 to 250 mu m; and calcining the microspheric particles to obtain the spherical wear-resistant nano calcium oxide-based C02 reaction adsorbent. The wear strength of the wear-resistant nano calcium oxide-based C02 reaction adsorbent can be improved by the method; and the method can be widely applied to industrial processes of removing the C02 from industrial smoke, removing the C02 by a reaction, a new adsorption strengthening methane steam reforming hydrogen production process and the like.
Chan, C.K., T.S.S. Kumar, S. Liao, R. Murugan, M. Ngiam and S. Ramakrishna, 2006. Biomimetic nanocomposir.es for bone graft applications. Nanomedicine, 1: 177-188.
Gross, K.A., J. Andersons, M. Misevicius and J. Svirksts, 2014. Traversing phase fields towards nanosized beta triealcium phosphate. Key Eng. Mater., 587: 97-100.
Weiss, J.,P. TakhistovandD.J. McClements, 2006.Functional materials in food nanotechnology. J. Food Sci., 71: R107-R116.

Swain, P.S., D. Rajendran, S.B. Rao and G. Dominic, 2015. Preparation and effects of nano mineral particle feeding in livestock: A review. Vet. World, 8: 888-891.
SUMMARY OF INVENTION
A nanoparticle is a piece of material that is so small it has to be measured in nanometers. One nanometer equals one-billionth of a meter. For comparison, 10,000 nanoparticles could fit in the diameter of a human hair .Nano particles refer to a particle size of roughly 1 to 100 nm .At this scale the physical, chemical and biological properties of material differ fundamentally and often unexpectedly. These nanomineral particles are having higher potential than their conventional sources and thus reduce the quantity required.Chickens need calcium. It's important for their egg production. Hens without enough of it in their diets can lay soft or no shell eggs. Fortunately. Nano calcium is having a great potential as performance enhancer in broiler chicken production even at very lower doses than the conventional organic and inorganic sources.
DRAWING:
Fig 1, Illustrating shows the flow chart of Nano Calcium in which Incubation period results in a change of color of the mixture to Light Brown which is a visual confirmation of the synthesized NPs . Further, synthesized nanoparticles are further characterized using Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM).
Fig 2, shows the SEM micrograph of Calcium oxide nanoparticles at 200 nm (nanometer) in which SEM technique was employed to visualize the size of calcium oxide nanoparticles. The formation of calcium oxide nanoparticles as well as their morphological dimensions in the SEM analysis demonstrated that the average size was from 78-1J 6 nm with inter-particle distance.

Fig 3, is Sample of Nano calcium oxide
Fig 4 , Shows the dual role of the plant extract as a reducing and capping agent and presence of some functional groups was confirmed by FTIR analysis . A broad band between 3441 cra-1 is due to the N-H stretching vibration of group NH2 and OH the overlapping of the stretching vibration of attributed for DMSO and plant extract molecules. The observed peaks at 1028 cm-1 denote -C-OC- linkages, or -C-O- bonds. The observed peaks are mainly attributed to flavanoids and terpenoids excessively present in plants extract. From FT-TR results, it can be concluded that some of the bioorganics compounds from plant extract formed a strong coating/capping on the nanoparticles.
DETAILED DESCRIPTION OF THE INVENTION
Preparation Of Plant Extract
The plant part is washed thoroughly in running tap water and sterilized using double distilled water Then, the plant part is kept for drying at room temperature followed by weighing and then crushing it using a mortar and pestle.
DMSO(Dimethyl Sulfoxide) is added to the plant part according to the desired concentration and continuous stirring using a magnetic stirrer. The solution is filtered using Whatman filter paper and the obtained clear solution was used as a plant extract.
Bio-availability of Ca
The major mineral, calcium is highly essential to maintain the skeletal structure. It is a divalent caption, which cannot be synthesized in the body and only way to get it is through food and supplementation. Thus it can be categorized as essential mineral like one of the essential amino acids. When the feed is deficient in calcium, the calcium will be drawn from the bones. Majority of the calcium in the animal and bird body is stored in bones .

Presently, added minerals are used as nanoparticles in order to increase absorption and subsequent decreased presence in poultry excreta. Chan et al.(2006) and Gross et al.(2014) have reported that calcium phosphate materials in nano-size have higher specific surface area and surface roughness compared to the conventional calcium phosphate. Weiss et al.(2006) reported that nanoparticle-sized ingredients might increase the functionality or bioavailability of ingredients and nutrients, thereby minimize the concentrations needed in the food product. Calcium phosphate materials in nano-size are expected to have better bioactivity compared to conventional materials because supplementation of minerals in nano form (Se, Cr and Zn) increases the bioavailability and efficiency of utilization by increasing the surface area. The usefulness of nano form in reducing the mineral quantify to half or more in the diet will reduce the cost of feeding when the actual production of calcium phosphate nano-particles is upsealed to an industrial level.
Advantages of NANO Ca
• Swain et o/.(2015) reported that nano minerals are having a great potential even at very lower doses than the conventional organic and inorganic sources.
• Nano calcium is having a great potential as performance enhancer in broiler chicken production even at very lower doses than the conventional organic and inorganic sources.
• Effective delivery of functional compounds to target sites and thereby better bioavailability.
• When broiler birds are fed witfi nano form of calcium by replacing upto 50% requirement of dicalcium phosphate, they showed a best feed conversion ratio and differed significantly from the control groups.
• Calcium is responsible for construction, formation and maintenance of bone and
teeth.
t
• This function helps reduce the occurrence of osteoporosis. It is also a vital
component inblood clotting systems and wound healing.

THE DEFICIENCES / DRAWBACKS IN THE PRESENT INVENTION
Particle size is an important factor for good mineral absorption, because a fundamental physical law states that the smaller the particle, the greater the relative particle surface. It is this surface that the body uses to break down and absorb nutrients. The larger this surface, the better nutrients can be utilized.
We CLAIM
1. A method of synthesizing calcium oxide nanoparticles, comprising the steps of providing a solution of calcium ions & providing plant extract solution and combining the calcium ion solution and the plant extract solution while stirring at a temperature of 40° C to produce the calcium oxide nanoparticles.
2. The method of synthesizing calcium oxide nanoparticles according to claim 1, where Calcium nitrate tetra hydrate (Ca(N03)2.4H20 ) used as a calcium ion source.
3. The method of synthesizing calcium oxide nanoparticles according to claim 1, where DMSO used as a solvent.
4. The method of synthesizing calcium oxide nanoparticles according to claim 1, where plant extract used as a reducing agent.
5. The method of synthesizing calcium oxide nanoparticles according to claim 1, wherein said calcium oxide nanoparticles have a mean diameter in the range from about 78 to about 116 nm.

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1 201941007616-US(14)-ExtendedHearingNotice-(HearingDate-19-01-2021).pdf 2021-10-17
1 Form9_Earlier Publication_27-02-2019.pdf 2019-02-27
2 201941007616-US(14)-HearingNotice-(HearingDate-11-12-2020).pdf 2021-10-17
2 Form3_As Filed_27-02-2019.pdf 2019-02-27
3 Form2 Title Page_Complete_27-02-2019.pdf 2019-02-27
3 201941007616-IntimationOfGrant15-03-2021.pdf 2021-03-15
4 Form1_As Filed_27-02-2019.pdf 2019-02-27
4 201941007616-PatentCertificate15-03-2021.pdf 2021-03-15
5 Form18_Normal Request_27-02-2019.pdf 2019-02-27
5 201941007616-PETITION UNDER RULE 137 [11-03-2021(online)].pdf 2021-03-11
6 Drawing_As Filed_27-02-2019.pdf 2019-02-27
6 201941007616-PETITION UNDER RULE 138 [11-03-2021(online)].pdf 2021-03-11
7 Description Complete_As Filed_27-02-2019.pdf 2019-02-27
7 201941007616-RELEVANT DOCUMENTS [11-03-2021(online)].pdf 2021-03-11
8 Correpondence by Applicant_As Filed_27-02-2019.pdf 2019-02-27
8 201941007616-EVIDENCE FOR REGISTRATION UNDER SSI [10-03-2021(online)].pdf 2021-03-10
9 201941007616-FORM FOR SMALL ENTITY [10-03-2021(online)].pdf 2021-03-10
9 Claims_As Filed_27-02-2019.pdf 2019-02-27
10 201941007616-OTHERS [10-03-2021(online)].pdf 2021-03-10
10 Abstract_As Filed_27-02-2019.pdf 2019-02-27
11 201941007616-ABSTRACT [02-02-2021(online)].pdf 2021-02-02
11 abstract 201941007616.jpg 2019-03-01
12 201941007616-CLAIMS [02-02-2021(online)].pdf 2021-02-02
12 201941007616-FER.pdf 2020-03-13
13 201941007616-COMPLETE SPECIFICATION [02-02-2021(online)].pdf 2021-02-02
13 201941007616-Proof of Right_First Examination Report_07-09-2020.pdf 2020-09-07
14 201941007616-CORRESPONDENCE [02-02-2021(online)].pdf 2021-02-02
14 201941007616-Form3_First Examination Report_07-09-2020.pdf 2020-09-07
15 201941007616-DRAWING [02-02-2021(online)].pdf 2021-02-02
15 201941007616-Drawing_First Examination Report_07-09-2020.pdf 2020-09-07
16 201941007616-Correspondence_First Examination Report_07-09-2020.pdf 2020-09-07
16 201941007616-FER_SER_REPLY [02-02-2021(online)].pdf 2021-02-02
17 201941007616-OTHERS [02-02-2021(online)].pdf 2021-02-02
17 201941007616-Abstract_First Examination Report_07-09-2020.pdf 2020-09-07
18 201941007616-Abstract_First Examination Report_07-09-2020.pdf 2020-09-07
18 201941007616-OTHERS [02-02-2021(online)].pdf 2021-02-02
19 201941007616-Correspondence_First Examination Report_07-09-2020.pdf 2020-09-07
19 201941007616-FER_SER_REPLY [02-02-2021(online)].pdf 2021-02-02
20 201941007616-DRAWING [02-02-2021(online)].pdf 2021-02-02
20 201941007616-Drawing_First Examination Report_07-09-2020.pdf 2020-09-07
21 201941007616-CORRESPONDENCE [02-02-2021(online)].pdf 2021-02-02
21 201941007616-Form3_First Examination Report_07-09-2020.pdf 2020-09-07
22 201941007616-COMPLETE SPECIFICATION [02-02-2021(online)].pdf 2021-02-02
22 201941007616-Proof of Right_First Examination Report_07-09-2020.pdf 2020-09-07
23 201941007616-CLAIMS [02-02-2021(online)].pdf 2021-02-02
23 201941007616-FER.pdf 2020-03-13
24 abstract 201941007616.jpg 2019-03-01
24 201941007616-ABSTRACT [02-02-2021(online)].pdf 2021-02-02
25 201941007616-OTHERS [10-03-2021(online)].pdf 2021-03-10
25 Abstract_As Filed_27-02-2019.pdf 2019-02-27
26 201941007616-FORM FOR SMALL ENTITY [10-03-2021(online)].pdf 2021-03-10
26 Claims_As Filed_27-02-2019.pdf 2019-02-27
27 201941007616-EVIDENCE FOR REGISTRATION UNDER SSI [10-03-2021(online)].pdf 2021-03-10
27 Correpondence by Applicant_As Filed_27-02-2019.pdf 2019-02-27
28 201941007616-RELEVANT DOCUMENTS [11-03-2021(online)].pdf 2021-03-11
28 Description Complete_As Filed_27-02-2019.pdf 2019-02-27
29 201941007616-PETITION UNDER RULE 138 [11-03-2021(online)].pdf 2021-03-11
29 Drawing_As Filed_27-02-2019.pdf 2019-02-27
30 201941007616-PETITION UNDER RULE 137 [11-03-2021(online)].pdf 2021-03-11
30 Form18_Normal Request_27-02-2019.pdf 2019-02-27
31 Form1_As Filed_27-02-2019.pdf 2019-02-27
31 201941007616-PatentCertificate15-03-2021.pdf 2021-03-15
32 Form2 Title Page_Complete_27-02-2019.pdf 2019-02-27
32 201941007616-IntimationOfGrant15-03-2021.pdf 2021-03-15
33 Form3_As Filed_27-02-2019.pdf 2019-02-27
33 201941007616-US(14)-HearingNotice-(HearingDate-11-12-2020).pdf 2021-10-17
34 Form9_Earlier Publication_27-02-2019.pdf 2019-02-27
34 201941007616-US(14)-ExtendedHearingNotice-(HearingDate-19-01-2021).pdf 2021-10-17

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