FIELD OF THE INVENTION
In June 1999, Richard Smalley, Nobel laureate in chemistry, addressed the US House
Committee on Science on the benefits of nanotechnology. "The impact of nanoteclmology on
the health, wealth, and lives of people," he said, "will be at least the equivalent of the
combined influences of microelectronics, medical imaging, computer-aided engineering and
man-made polymers developed in this century."
Recent advances in the field of nanotechnology, particularly the ability to prepare highly
ordered nanoparticulates of any size and shape, have led to the development of new biocidal
agents. Nano-materials are called "a wonder of modern medicine". It is stated that antibiotics
kill perhaps a half dozen different disease-causing organisms but nano-materials can kill
plentiful.
Metal Nanoparticles have various functions that are not observed in bulk phase and have been
studied extensively because of their exclusive catalytic, optical, electronic, magnetic and
antimicrobial wound healing and anti-inflammatory properties . Over the past few decades,
inorganic Nanoparticles, whose structures exhibit significantly novel and improved physical,
chemical, and biological properties and functionality due to their nano-scale size, have
elicited much interest.
I
OBJECT OF INVENTION
i |
i i
Biosynthesis of Nanoparticles is an approach of synthesizing Nanoparticles using plants having biomedical applications. This approach is an environment-friendly, cost-effective, biocompatible, safe, green approach .Green synthesis includes synthesis through plants, bacteria, fungi, algae etc. They allow large scale production of ZnO NPs free of additional impurities. NPs synthesized from biomimetic approach show more catalytic activity and limit the use of expensive and toxic chemicals.These plant extract secrete some phytochemicals that act as both reducing agent and capping or stabilization agent.

Plant parts like leaf, stem, root, fruit, and seed have been used for ZnO NPs synthesis because of the exclusive phytochemicals that they produce. Using natural extracts of plant parts is a very eco-friendly, cheap process and it does not involve usage of any intermediate base groups. It takes very less time, does not involve usage of costly equipment and precursor and gives a highly pure and quantity enriched product free of impurities .
Plants are most preferred source of NPs synthesis because they lead to large-scale production and production of stable, varied in shape and size NPs . Bio-reduction involves reducing metal ions or metal oxides to 0 valence metal NPs with the help of phytochemicals like polysaccharides, polyphenolic compounds, vitamins, amino acids, alkaloids, terpenoids secreted from the plant.
BACKGROUND OF THE INVENTION
Zn is a trace element that is necessary for a healthy immune system. A lack of zn can make a birds and animal more susceptible to disease and illness.
Zinc activates more than 100 enzymes - the class of proteins that carry out chemical reactions, such as those that drive metabolism. Getting enough zinc helps the body make protein by taking the amino acids obtained from nutrition and then assembling those amino acids into new proteins that contribute to tissue function. Zinc also affects the cells' metabolism by helping cells regulate gene activity. This allows cells to activate genes required for metabolic chemical reactions and then turn those genes back off when they're no longer needed.
Nanoparticles compositions are useful in a variety of contexts. Nanoparticles compositions have proven to be particularly useful and/or effective in the context of medical applications, including administering therapeutic agents to patients in need thereof.

PRIOR ART
Patent application number 201818038901
The present invention describes novel Nanoparticles compositions, and systems and methods utilizing them for treating disorders and/or conditions. Methods generally involve administering Nanoparticles compositions (e.g., Nanoparticles compositions comprising at least one known therapeutic agent and/or independently active biologically active agent; and/or empty Nanoparticles compositions) to a subject in need thereof.
Patent application number 201841011292
A simplified and cost effective method for analysis and ascertaining various critical parameters like Ultrasonic Velocity values, Adiabatic Compressibility, Intramolecular Free length and Acoustical Impedence is provided. Metal and metal oxide Nanoparticles such as Zinc Oxide (ZnO), Copper Oxide (CuO), Silver (Ag) and Gold (Au) by chemical means and the Nanoparticles were characterized using X-ray diffraction (XRD), Scanning Electron Microscope (SEM), Energy Dispersive X-ray Spectroscopy (EDX) and Transmission Electron Microscope (TEM). Ultrasonic and thermal conductivity analysis of the nano particle were performed for understanding the particle-fluid and particle-particle interactions. Further, It was inferred, that the acoustical parameters such as adiabatic compressibility (Bad), Intermolecular free length (Lf), Acoustic impedance (Z) show that the metallic nanofluid has better response over the metal oxide nanofluids and specifically, Ag and Au nanofluids show the higher adiabatic compressibility and lower Acoustic impedance values which ensure that the interaction between the particle-fluid and particle-particle are stable.
Patent application number 201841003546
A method of synthesizing zinc oxide nano sized particle using green plant extracts and their use along with biodiesel for improving the fuel efficiency of diesel engine is described. The fuel composition comprising of methyl ester extracted from jatropha raw oil along with specific concentration of nano-sized zinc oxide Nanoparticles is introduced as fuel into a system comprising a diesel engine works on internal combustion engine principle. The inclusion of nano-sized particle enhances the fuel properties such as cetane number, hearing value and flash point. The presence of Nanoparticles in biodiesel improves the brake thermal efficiency of diesel engine. The exhaust emissions of diesel engine such as carbon monoxide, unburnt hydrocarbons and smoke opacity is substantially minimized by the addition of synthesized zinc oxide Nanoparticles.
Patent application number 201741037131
Title: Nano CeOz-Tioz Composite Incorporated Al-Zn Alloy Sacrificial Anode Resistant To
Biofouling A nano CeOz-Tioz composite incorporated sacrificial anode that is non-toxic, has

high current carrying capacity, is resistant to biofouling and possesses uniform corrosion morphology, and the process of manufacture is disclo_sed herein. Said sacrificial anode comprises of nano CeOz-Tioz composite in the range of 0.080 - 0.120% by weight, 5% zinc by weight, and eest aluminium by weight, wherein the nano CeOz-Tioz composite is homogenously mixed in the Al-Zn alloy. FIG.3
Patent application number 5169/CHE/2014 ,
Nanomaterials are cornerstones of nanoscience and nanoteehnology.Nanoparticles research is currently an area of intense scientific interest due to a wide variety of potential applications in biomedical, optical and electronic fields. Green and novel nano size Zinc oxide particle were synthesized from the plant source by efficient slightly modified combustion method. The peak obtained from the FT-IR spectrum is the characteristic peak of Zn-0 bond. The powder X-ray diffraction study for the synthesized ZnO Nanoparticles suggests hexagonal wurzite structure for it. The particle size was identified by the Debye-Scherer formula and found to be in the range of 35nm. The morphology was analyzed by SEM and it was found that the synthesized Nanoparticles wre in the form of clusters. The EDX measurements confirmed the composition of Zn and 0 in the synthesized ZnO nano particle. The potential efficacies against the bacterial and funga! strains were studied for the ZnO Nanoparticles synthesized from the green route. The study ascertains that the synthesized ZnO nano particle have a good efficacy against both bacterial and fungal organisms.
Patent application number US20120097522A1
A simple, room-temperature method of producing, zinc oxide Nanoparticles was established by reacting zinc nitrate hexahydrate and cyclohexylamine (CHA) in either aqueous or EtOHic medium. Particle of polyhedra morphology were obtained for zinc oxide, prepared in EtOH (ZnOE), while an irregular spherical morphology,.mixed with some chunky particle forzinc oxide prepared in water (ZnOW). The results indicate that there are significant morphological differences between ZnOE and ZnOW. ZnOE showed a regular polyhedral shape, while spherical and chunky particle were observed for ZnOW. The morphology was crucial in enhancing the cyanide ion photocatalyric degradation efficiency of ZnOEby a factor of 1.5 in comparison to the efficiency of ZnOW at equivalent loading of 0.02 ZnO Nanoparticles wt %. Increasing the loading wt % of ZnOE from 0.01 to 0.07 led to an increase in the photocatalytic degradation efficiency from 67% to 90% after 45 minutes and a doubling of the first-order rate constant (k).
Patent application number CN102691205A
The invention discloses a method using a hydrothermal method to prepare nano-zinc-oxide assembly cotton fibers and particularly relates to a method using a hydrothermal method twice to prepare nano-zinc-oxide assembly cotton fibers with functions of fastness to washing and ultraviolet resistance. The method includes the steps: pre-treating cotton serving as main raw materials by NaOH solution to remove non-cellulosic components on the cotton fibers; then putting the pre-treated cotton fibers into initial growth medium composed of zinc nitrate and

hexamethylene tetramine, transferring mixed liquor into a reaction kettle for reaction for 2h at the temperature of 60-100 DEG C after ultrasonic dispersion, and cleaning the cotton fibers with distilled water; and finally, putting the cleaned cotton fibers into newly-prepared zinc salt growth medium for reaction for 6-1 Oh at the temperature of 60-100 DEG C so that the nano-zinc-oxide assembly cotton fibers are prepared. The method is simple in operation, and the growth medium for treating the cotton fibers can be recovered and can be returned to the cotton fiber procedures for reuse after concentration adjustment of the zinc nitrate and the hexamethylene tetramine, so that cost is reduced.
Patent application number CNI03320827B
The present invention relates to a method for the preparation of nano zinc oxide film doped with an inorganic salt, comprises the following procedure: First, the configuration precursor solution, the zinc source is zinc nitrate or zinc chloride, the inorganic salt is sodium chloride or sodium nitrate, in zinc salt solution was added dropwise a certain amount of an inorganic salt; solution was then transferred to the body three-electrode system, wherein a conductive substrate as a working electrode, a platinum electrode as the auxiliary electrode, a saturated calomel electrode as the reference electrode, the deposition temperature of 25 -50 deg.] C, deposition time 5-20min, deposition potential of-0.9V - -1,5 V; performed after the last treatment, the product was rinsed, dried, calcined deg.] C 500 Ih.The method of the present invention is simple, low cost, preparation of product as a white, lead-zinc hexagonal structure.
I Patent application Title "Green synthesized ZnO Nanoparticles against bacterial and fungal
pathogens'
Zinc oxide Nanoparticles are known to be one of the multifunctional inorganic Nanoparticles with effective antibacterial activity. This study aims to determine the antimicrobial efficacy of green and chemical synthesized ZnO Nanoparticles against various bacterial and fungal pathogens. Various microbiological tests were performed using varying concentrations of green and chemical ZnO NPs with sizes 40 and 25 nm respectively. Results prove that green ZnO Nanoparticles show more enhanced biocidal activity against various pathogens when compared to chemical ZnO Nanoparticles. Also effectiveness of Nanoparticles increases with increasing particle dose, treatment time and synthesis method. In addition, the current study has clearly demonstrated that the particle size variation and surface area to volume ratio of green ZnO Nanoparticles are responsible for significant higher antimicrobial activity. From the results obtained it is suggested that green ZnO NPs could be used effectively in agricultural and food safety applications and also can address future medical concerns.

SUMMARY OF INVENTION
A Nanoparticles is a piece of material that is so small it has to be measured in nanometers. One nanometer equals one-billionth of a meter. For comparison, 10,000 Nanoparticles could fit in the diameter of a human hair .Nano particle refer to a particle size of roughly 1 to 100 nm .At this scale the physical, chemical and biological properties of material differ fundamentally and often unexpectedly. These nanomineral particle are having higher potential than their conventional sources and thus reduce the quantity required.
DRAWING:
Fig 1, Shows the flow chart of Nano ZNo in which Incubation period results in a change of color of the mixture to Light Brown which is a visual confirmation of the synthesized NPs . Further, synthesized Nanoparticles are further characterized using Fourier Transform Infrared Spectroscopy (FTIR), & Scanning Electron Microscopy (SEM)
Fig 2, Shows the microscopic view at 200 nm (nanometer) in which I
i
SEM technique was employed to visualize the size of zinc oxide Nanoparticles. The i
formation of zincoxide Nanoparticles as well as their morphological dimensions in the j
i
SEM analysis demonstrated that the average size was from 63-152 nm with inter-particle j
distance. j
Fig 3, Shows the Sample - Nano Zinc oxide
Fig 4, Shows The dual role of the plant extract as a reducing and capping agent and presence of some functional groups was confirmed by FTIR analysis . A broad band between 3441 cm-1 is due to the N-H stretching vibration of group NH2 and OH the overlapping of the stretching vibration of attributed for DMSO and plant extract molecules. The observed peaks at 1028 cm-1 denote -C-OC- linkages, or-C-O- bonds. The observed peaks are mainly attributed to flavanoidsand terpenoidsexcessively present in plants extract. From FT-IR results, it can be concluded that some of the bioorganics compounds from plant extract formed a strong coating/capping on the Nanoparticles.

DETAILED DESCRIPTION OF THE INVENTION
PREPARATION OF PLANT EXTRACT
The plant part is washed thoroughly in running tap water and sterilized using double distilled water Then, the plant part is kept for drying at room temperature followed by weighing and then crushing it using a mortar and pestle.
DMSO(Dimethyi Sulfoxide) is added to the plant part according to the desired concentration and continuous stirring using a magnetic stirrer. The solution is filtered using Whatman filter paper and the obtained clear solution was used as a plant extract.
BIO-AVAILABlLiTY OF ZN
Absorption of Zn in the body is very less and differs with the age of the birds and animal and the sites in the gastrointestinal tract. Zn can be incorporated in the diet as inorganic salts like ZnO and Zn Sulphate (ZnS04) and as organic chelates such as Zn propionate and Zn acetate. Even though, the bioavailability of Zn in organic sources is higher than that of inorganic Zn salts, the use of organic Zn chelates in animal and birds diets is limited due to its higher cost. Higher levels of Zn excreted from the supplemented birds and animals have raised concerns pertaining to environmental pollution .Thus, this problem opens a window for better bio-available Zn sources and if possible, to reduce the supplemental dose of Zn to the animal food. Among all the probable approaches, use of nanotechnology to produce nano sized Zn sailed as nanoZnO is a potential alternative to both organic and inorganic Zn sources. The use of nanoZnO has shown to produce better results as compared with conventional Zn sources.

ADVANTAGES OF NANO ZNO
• Nano particle refer to a particle size of roughly 1 to 100 nm .At this scale the physical, chemical and biological properties of material differ fundamentally and often unexpectedly. These nanomineral particle are having higher potential than their conventional sources and thus reduce the quantity required.
• It promotes growth can act as antibacterial agent, modulates the immunity and reproduction of the birds and animals.
• These can be used at lower doses and can provide better result than the conventional Zn sources and indirectly prevents environmental contamination also.
• Zinc oxide Nanoparticles are being used in the feed industry as additives and during packaging due to their antimicrobial properties .
THE DEFICIENCES / DRAWBACKS IN THE PRESENT INVENTION
Particle size is an important factor for good mineral absorption, because a fundamental physical law states that the smaller the particle, the greater the relative particle surface. It is
this surface that the body uses to break down and absorb nutrients. The larger this surface, the better nutrients can be utilized.

CLAIMS We CLAIM
1. A method of synthesizing zinc oxide nanoparticles, comprising the steps of providing a solution of zinc ions & providing plant extract solution and combining the zinc ion solution and the plant extract solution while stirring at a temperature of 40° C to produce the zinc oxide nanoparticles.
2. The method of synthesizing zinc oxide nanoparticles according to claim 1, where Zinc nitrate hexahydrate (Zn(N03)2.6H20 ) used as a zinc ion source.
3. The method of synthesizing zinc oxide nanoparticles according to claim 1, where DMSO used as a solvent.
4. The method of synthesizing zinc oxide nanoparticles according to claim 1, where plant extract used as a reducing agent.
5. The method of synthesizing zinc oxide nanoparticles according to claim 1, wherein said zinc oxide nanoparticles have a mean diameter in the range from about 63 to about 152 nm.