DESC:FIELD OF THE INVENTION
[0001] The present disclosure relates to a toilet cleaner concentrate composition. Specifically, the present disclosure provides a toilet cleaner concentrate composition comprising a natural disinfectant, an anti-microbial agent, a surfactant, a thickening agent and a solvent. Said composition has cleansing and anti-microbial properties. The present disclosure further relates to a process for preparing the toilet cleaner concentrate composition.

BACKGROUND OF THE INVENTION
[0002] The background description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art.
[0003] Toilets are fixtures that are everyday use for humans and involve physical contact with human skin. Since fecal matter and other impurities are constantly fed into toilets they are always exposed to germs and other microbes. Therefore, maintenance of toilets is an essential requirement to avoid microbes and germs from passing onto humans and causing infectious diseases. Some of the diseases that can spread through toilets are common cold, gut infections for example from E. coli, skin or vaginal infections etc.
[0004] Toilet cleaners need to be not just effective cleansers but must also be effective disinfectants to curb the growth of micro-organisms. Many compositions have been known in the past for toilet cleansing some in the liquid form or some in the form of powders. However, both these forms are associated with some drawbacks. Liquid cleaners which are used directly contain very harsh chemicals that may soil the toilet fixtures and also consume a lot of water in the cleaning process. Additionally, these cleaners may be associated with strong fumes that may be dangerous for kids or pets in the house. For powder cleaners it may be difficult to gauge how much of the powder must be used for effective cleaning of toilets. Powders are also known to be moisture absorbers that may lead to their inactivity. Usage of these powders in all climates and regions may not be very feasible.
[0005] Another problem with existent cleaners is their usage of a great number of toxic chemicals for example strong acids, bleaches or chlorine etc. These are harmful corrosive chemicals that may cause problems on spillage or accidental exposure to humans, especially kids. Additionally, these chemicals may also corrode or damage the bowl of toilets.
[0006] Therefore, there is a need in the art to provide unique toilet cleaner compositions that are safe, and effective. These compositions should not just be capable of cleaning but should also provide quick anti-microbial effect. The composition devised by the inventors of the present disclosure help overcome these drawbacks in the art.

OBJECTS OF THE INVENTION
[0007] An object of the present disclosure is to provide a toilet cleaner concentrate composition.
[0008] An object of the present disclosure is to provide a toilet cleaner concentrate composition with cleansing, deodorizing as well as disinfectant properties.
[0009] An object of the present disclosure is to provide a toilet cleaner concentrate composition free of chemicals such as hydrochloric acid, parabens, phenolic compound, and phenyl.
[0010] An object of the present disclosure is to provide an acid-free toilet cleaner concentrate composition with excellent solubility in tap water.

SUMMARY OF THE INVENTION
[0011] This summary is provided to introduce a selection of concepts in a simplified form that are further described below in Detailed Description section. This summary is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used as an aid in determining the scope of the claimed subject matter.
[0012] The present disclosure relates to a toilet cleaner concentrate composition. Specifically, the present disclosure provides a toilet cleaner concentrate composition, comprising safe components and having cleansing, deodorizing and disinfectant properties.
[0013] In one aspect, the present disclosure provides a toilet cleaner concentrate composition comprising a natural disinfectant, an anti-microbial agent, a surfactant, hydroxypropyl methylcellulose as a thickening agent, and glycerol as a solvent.
[0014] In an embodiment, the present disclosure provides a toilet cleaner concentrate composition comprising a natural disinfectant, an anti-microbial agent, a surfactant, hydroxypropyl methylcellulose as a thickening agent, and glycerol as a solvent; wherein the hydroxypropyl methylcellulose is present in a range of about 8% w/w to about 15% w/w of the composition and wherein the glycerol is present in a range of about 45% w/w to about 55% w/w of the composition.
[0015] In an embodiment, the natural disinfectant may be selected from neem oil, neem extract, lavender oil, lemon oil, lemon extract, vinegar, tea tree oil, peppermint oil, eucalyptus oil, tulsi extract, orange extract, or combinations thereof.
[0016] In an embodiment, the anti-microbial agent may be selected from polyvinylpyrrolidone-iodine, chlorhexidine, ammonium compound, or iodine compound.
[0017] In an embodiment, the surfactant may be selected from sodium stearate, sodium coco sulfate, coco glucoside, decyl glucoside, sodium lauryl sulfate, ammonium lauryl sulfate, ammonium laureth sulfate, cocoamphocarboxy glycinate, sodium lauryl sulfoacetate, sodium pareth sulfate, sodium myreth sulfate, alpha olefin sulfonate, sodium lauroyl sarcosinate, polysorbate 20, polysorbate 80, triethanolamine lauryl sulphate, cocamide diethanolamine (DEA), cocamidopropyl betaine or combinations thereof.
[0018] In an embodiment, the composition may further comprise one or more additive(s). The additives may be selected from foam stabilizer, preservatives, color, fragrance or combinations thereof.
[0019] In an embodiment, the foam stabilizer may be selected from sodium stearate, sodium coco sulfate, sodium lauryl sulfoacetate, sodium lauroyl sarcosinate, sodium pareth sulfate, alpha olefin sulfonate, sodium lauryl sulphate, triethanolamine lauryl sulphate, cocoamido propyl betaine, cocoamide DEA or combinations thereof.
[0020] In an embodiment, the preservative may be selected from tetrasodium EDTA, disodium EDTA, citric acid, sodium benzoate, sodium citrate, sorbic acid, acetic acid, benzoic acid, butylated hydroxytoluene or combinations thereof.
[0021] In another aspect, the present disclosure provides a toilet cleaner concentrate formulation comprising a natural disinfectant, an anti-microbial agent, a surfactant, hydroxypropyl methylcellulose as a thickening agent, glycerol as a solvent, and one or more additive(s); wherein the hydroxypropyl methylcellulose is present in a range of about 8% w/w to about 15% w/w of the formulation and wherein the glycerol is present in a range of about 45% w/w to about 55% w/w of the formulation.
[0022] In yet another aspect, the present disclosure provides a process for preparing a toilet cleaner concentrate composition, wherein the process comprises the steps of: (a) mixing liquid components of the composition in a solvent till homogenous to give a pre-mixture A; (b) grinding and mixing solid components of the composition till homogenous to give pre-mixture B; and (c) blending the pre-mixture A and pre-mixture B by stirring till uniform homogenous composition is obtained; wherein the composition comprises a natural disinfectant, an anti-microbial agent, a surfactant, hydroxypropyl methylcellulose as a thickening agent, and glycerol as a solvent; wherein the hydroxypropyl methylcellulose is present in a range of about 8% w/w to about 15% w/w of the composition and wherein the glycerol is present in a range of about 45% w/w to about 55% w/w of the composition.
[0023] In still another aspect, the present disclosure provides a method of cleaning a toilet surface with the toilet cleaner concentrate composition.
[0024] Other aspects of the invention will be set forth in the description which follows, and in part will be apparent from the description, or may be learnt by the practice of the invention.

DETAILED DESCRIPTION
[0025] The following is a detailed description of embodiments of the disclosure. The embodiments are in such detail as to clearly communicate the disclosure. However, the amount of detail offered is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the present disclosure as defined by the appended claims.
[0026] All publications herein are incorporated by reference to the same extent as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference. Where a definition or use of a term in an incorporated reference is inconsistent or contrary to the definition of that term provided herein, the definition of that term provided herein applies and the definition of that term in the reference does not apply.
[0027] Various terms as used herein are shown below. To the extent a term used in a claim is not defined below, it should be given the broadest definition persons in the pertinent art have given that term as reflected in printed publications and issued patents at the time of filing.
[0028] Unless the context requires otherwise, throughout the specification and claims which follow, the word “comprise” and variations thereof, such as, “comprises” and “comprising” are to be construed in an open, inclusive sense that is as “including, but not limited to.”
[0029] Reference throughout this specification to “one embodiment” or “an embodiment” means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrases “in one embodiment” or “in an embodiment” in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
[0030] As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” include plural referents unless the content clearly dictates otherwise. It should also be noted that the term “or” is generally employed in its sense including “and/or” unless the content clearly dictates otherwise.
[0031] In some embodiments, the numbers expressing quantities of ingredients, properties such as concentration, reaction conditions, and so forth, used to describe and claim certain embodiments of the invention are to be understood as being modified in some instances by the term “about.” Accordingly, in some embodiments, the numerical parameters set forth in the written description are approximations that can vary depending upon the desired properties sought to be obtained by a particular embodiment. In some embodiments, the numerical parameters should be construed in light of the number of reported significant digits and by applying ordinary rounding techniques. Notwithstanding that the numerical ranges and parameters setting forth the broad scope of some embodiments of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as practicable. The numerical values presented in some embodiments of the invention may contain certain errors necessarily resulting from the standard deviation found in their respective testing measurements.
[0032] The recitation of ranges of values herein is merely intended to serve as a shorthand method of referring individually to each separate value falling within the range. Unless otherwise indicated herein, each individual value is incorporated into the specification as if it were individually recited herein.
[0033] All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g. “such as”) provided with respect to certain embodiments herein is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention otherwise claimed. No language in the specification should be construed as indicating any non-claimed element essential to the practice of the invention.
[0034] The headings and abstract of the invention provided herein are for convenience only and do not interpret the scope or meaning of the embodiments.
[0035] The following discussion provides many example embodiments of the inventive subject matter. Although each embodiment represents a single combination of inventive elements, the inventive subject matter is considered to include all possible combinations of the disclosed elements. Thus if one embodiment comprises elements A, B, and C, and a second embodiment comprises elements B and D, then the inventive subject matter is also considered to include other remaining combinations of A, B, C, or D, even if not explicitly disclosed.
[0036] The present disclosure relates to a toilet cleaner concentrate composition. Specifically, the present disclosure relates to a toilet cleaner concentrate composition, comprising natural disinfectants and having cleansing, deodorizing and anti-microbial properties.
[0037] In an embodiment, the present disclosure provides a toilet cleaner concentrate composition comprising a natural disinfectant, an anti-microbial agent, a surfactant, a thickening agent, and a solvent.
[0038] In an embodiment, the present disclosure provides a toilet cleaner concentrate composition comprising a natural disinfectant, an anti-microbial agent, a surfactant, hydroxypropyl methylcellulose as a thickening agent, and glycerol as a solvent; wherein the hydroxypropyl methylcellulose is present in a range of about 8% w/w to about 15% w/w of the composition and wherein the glycerol is present in a range of about 45% w/w to about 55% w/w of the composition.
[0039] In an embodiment, the natural disinfectant may be selected from neem oil, neem extract, lavender oil, lemon oil, lemon extract, vinegar, tea tree oil, peppermint oil, eucalyptus oil, tulsi extract, orange extract, or combinations thereof. In a preferred embodiment, the natural disinfectant may be neem extract.
[0040] The disinfectants employed are natural and safe to use as they are devoid of any harmful chemicals.
[0041] In an embodiment, the natural disinfectant may be present in a range from about 0.5%w/w to about 12%w/w, preferably about 1%w/w to about 10%w/w of the composition.
[0042] In an embodiment, the anti-microbial agent may be selected from polyvinylpyrrolidone-iodine, chlorhexidine, ammonium compound, or iodine compound. In a preferred embodiment, the anti-microbial agent may be polyvinylpyrrolidone-iodine.
[0043] In an embodiment, the anti-microbial agent may be present in a range from about 0.5%w/w to about 12%w/w, preferably about 1%w/w to about 10%w/w of the composition.
[0044] In an embodiment, the surfactant may be selected from sodium stearate, sodium coco sulfate, coco glucoside, decyl glucoside, sodium lauryl sulfate, ammonium lauryl sulfate, ammonium laureth sulfate, cocoamphocarboxy glycinate, sodium lauryl sulfoacetate, sodium pareth sulfate, sodium myreth sulfate, alpha olefin sulfonate, sodium lauroyl sarcosinate, polysorbate 20, polysorbate 80, triethanolamine lauryl sulphate, cocamide DEA, cocamidopropyl betaine or combinations thereof.
[0045] In a preferred embodiment, the surfactant may be cocamide diethanolamine (CDEA), cocamidopropyl betaine (CAPB), sodium lauryl sulfate (SLS), triethanolamine lauryl sulphate (TLS), or combinations thereof.
[0046] In an embodiment, the surfactant may be present in a range from about 0.5%w/w to about 25% w/w, preferably about 10%w/w to about 25%w/w of the composition.
[0047] In a preferred embodiment, the thickening agent may be present in a range of about 9%w/w to about 12%w/w of the composition.
[0048] In a preferred embodiment, the solvent may be present in a range of about 47%w/w to about 50% w/w of the composition.
[0049] In an embodiment, the composition may further comprise one or more additive(s). The additives may be selected from foam stabilizer, preservatives, color, fragrance or combinations thereof. However, a person of skill in the art would understand that any other additive(s) well-known in the art may be employed without going beyond the spirit and scope of the present disclosure.
[0050] In an embodiment, the foam stabilizer may be selected from sodium stearate, sodium coco sulfate, sodium lauryl sulfoacetate, sodium lauroyl sarcosinate, sodium pareth sulfate, alpha olefin sulfonate, sodium lauryl sulphate, triethanolamine lauryl sulphate, cocoamido propyl betaine, cocamide diethanolamine or combinations thereof. However, a person of skill in the art would understand that any other foam stabilizer(s) well-known in the art may be employed without going beyond the spirit and scope of the present disclosure.
[0051] In an embodiment, the preservative may be selected from tetrasodium EDTA, disodium EDTA, citric acid, sodium benzoate, sodium citrate, sorbic acid, acetic acid, benzoic acid, butylated hydroxytoluene or combinations thereof. However, a person of skill in the art would understand that any other preservative(s) well-known in the art may be employed without going beyond the spirit and scope of the present disclosure.
[0052] In an embodiment, the preservative may be present in a range from about 0.01%w/w to about 5% w/w, preferably about 0.02%w/w to about 2%w/w of the composition.
[0053] In an embodiment, the color may be selected from natural colors, green chlorophyll, indigo powder, deep blue color, yellow color, violet color, beetroot powder, sandalwood powder, or combinations thereof. However, a person of skill in the art would understand that any other color(s) well-known in the art may be employed without going beyond the spirit and scope of the present disclosure.
[0054] In an embodiment, the fragrance may be selected from lemon, jasmine, rose, mulberry, hibiscus, lily, lavender, or combinations thereof. However, a person of skill in the art would understand that any other fragrance(s) well-known in the art may be employed without going beyond the spirit and scope of the present disclosure.
[0055] In an embodiment, the composition is dissolved in ordinary water to give a solution suitable for toilet cleaning. In an embodiment, the composition dissolves in water quickly and is stable in tap water as well as reverse-osmosis (RO) water.
[0056] In a preferred embodiment, the present disclosure relates to a toilet cleaner concentrate composition comprising neem extract, polyvinylpyrrolidone-iodine, sodium lauryl sulfate, triethanolamine lauryl sulphate, hydroxypropyl methylcellulose, glycerol, and tetrasodium EDTA.
[0057] In another preferred embodiment, the present disclosure relates to a toilet cleaner concentrate composition comprising neem extract, polyvinylpyrrolidone-iodine, cocamide diethanolamine, cocamidopropyl betaine, hydroxypropyl methylcellulose, glycerol, and tetrasodium EDTA.
[0058] In an embodiment, the present disclosure relates to a toilet cleaner concentrate composition that is easily soluble in water. The composition forms a viscous solution. In an embodiment, the composition when dissolved in 500mL of water has a viscosity in the range of about 100 centipoise (cps) to about 450 cps.
[0059] In a preferred embodiment, the composition has a pH in the range of about 2.5 to about 5.0 in 500ml of water, preferably in the range of about 3.0 to 4.5 in 500ml of water.
[0060] In an embodiment, the composition is free of harmful chemicals, including strong acids such as hydrochloric acid, parabens, phenolic compounds, and phenyl. Thus the composition does not generate dangerous fumes and is safe for use around kids and pets. The absence of harsh chemicals also avoids corrosion of toilet bowls.
[0061] In an embodiment, the composition shows a synergistic anti-microbial activity. In an embodiment, the composition has excellent cleaning properties along with anti-microbial or disinfectant activity and deodorizing property. In some embodiment, the disinfectant activity is up to 99.99% even in a short duration of about 20 seconds. Thus, the composition has quick and strong activity.
[0062] In an embodiment, the composition has excellent solubility and stability. This concentrate can be easily dissolved in water providing good solubility, disinfectant activity, cleansing activity, good viscosity, no precipitation, and long lasting deodorizing effect.
[0063] The combination of the components of the composition has been engineered with glycerol and hydroxypropyl methyl cellulose to synergistically achieve the desired viscosity, stability, and solubility. The concentrate composition incorporating the properties of anti-microbial activity, safety, cleansing activity, good stability, and good viscosity has not been achieved in other compositions known in the art.
[0064] In an embodiment, the composition can effectively inhibit or destroy a plurality of widely known disease causing microorganisms found on toilet surfaces including gram-positive bacteria, gram-negative bacteria, fungus or virus. In a preferred embodiment, the composition is effective against Candida albicans, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Salmonella abony, Aspergillus brasilensis, and Listeria monocytogenes. Thus, the composition has broad spectrum activity.
[0065] In an embodiment, the present disclosure relates to a toilet cleaner concentrate composition that can be used for all kinds of toilet bowls including, but not limited to, ceramic, porcelain, clay, concrete, plastic, or wood.
[0066] In an embodiment, the present disclosure provides a toilet cleaner concentrate formulation comprising a natural disinfectant, an anti-microbial agent, a surfactant, hydroxypropyl methylcellulose as a thickening agent, glycerol as a solvent, and one or more additive(s); wherein the hydroxypropyl methylcellulose is present in a range of about 8% w/w to about 15% w/w of the formulation and wherein the glycerol is present in a range of about 45% w/w to about 55% w/w of the formulation.
[0067] In an embodiment, the formulation may be dissolved in water to give a ready-to-use formulation. In a preferred embodiment, about 25 mL of the formulation may be directly transferred to about 500 mL of water to give a ready-to-use formulation. The ready-to-use formulation may be directly sprayed and left for about 1-2 minutes before washing with brush and water.
[0068] The ready-to-use formulations available in the market are formulated using distilled water which is ordinarily produced using tap water. As per data, production of 2.5L of distilled water wastes approximately 200L of tap water. Therefore, approx. 1L of distilled water consumes 80L of tap water. The present disclosure does not require distilled water for its production. The ready to use formulation can be easily prepared in tap water or reverse osmosis (RO) water.
[0069] In still another embodiment, the present disclosure provides a process for preparing a toilet cleaner concentrate composition, wherein the process comprises the steps of: (a) mixing liquid components of the composition in a solvent till homogenous to give a pre-mixture A; (b) grinding and mixing solid components of the composition till homogenous to give pre-mixture B; and (c) blending the pre-mixture A and pre-mixture B and stirring till uniform homogenous composition is obtained; wherein the composition comprises a natural disinfectant, an anti-microbial agent, a surfactant, hydroxypropyl methylcellulose as a thickening agent, and glycerol as a solvent; wherein the hydroxypropyl methylcellulose is present in a range of about 8% w/w to about 15% w/w of the composition and wherein the glycerol is present in a range of about 45% w/w to about 55% w/w of the composition.
[0070] In an embodiment, the mixing may be performed by stirring with a rod or a homogenizer.
[0071] The composition obtained from this process may be closed and stored in air tight bottles at room temperature.
[0072] In an embodiment, the present disclosure provides a method of cleaning a toilet surface with the toilet cleaner concentrate composition.
[0073] In an embodiment, the composition is dissolved in water before cleaning the toilet surface.
[0074] In an embodiment, the composition may be diluted to about 90%, about 80%, about 75%, about 65%, about 60%, about 55%, about 50%, about 45%, about 40%, about 30%, about 20%, about 10% or about 5% of its strength.
[0075] While the foregoing describes various embodiments of the disclosure, other and further embodiments of the disclosure may be devised without departing from the basic scope thereof. The scope of the invention is determined by the claims that follow. The invention is not limited to the described embodiments, versions or examples, which are included to enable a person having ordinary skill in the art to make and use the invention when combined with information and knowledge available to the person having ordinary skill in the art.
EXAMPLES
[0076] The disclosure will now be illustrated with working examples, which is intended to illustrate the working of disclosure and not intended to take restrictively to imply any limitations on the scope of the present disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods and compositions, the exemplary methods, devices and materials are described herein. It is to be understood that this disclosure is not limited to particular methods, and experimental conditions described, as such methods and conditions may vary.
Materials: All raw materials were in house (IH) grades and commercially purchased from third party manufacturers.
Example 1
1.1 Toilet cleaner concentrate composition A
[0077] The concentrate composition was prepared as per proportions provided in Table No. 1 below. For pre-mixture A, HPMC was added in a clean and oven dried beaker, to which glycerol was added and mixed with a stirring rod or in a homogenizer till uniformity was achieved. TLS was added to this beaker and mixed with stirring rod/homogenizer till uniformity was achieved. For pre-mixture B, SLS, PVP, EDTA, coloring agent, neem and tulsi extracts were taken in another clean and oven-dried beaker and ground by crushing till uniformity was achieved. Pre-mixtures A and B were mixed together using a stirring road or homogenizer till uniformity was achieved. Fragrance was added and mixed to give the composition. The composition was kept tightly closed (avoiding air contact) at room temperature till further use.
Table No. 1: Toilet Cleaner Concentrate Composition
S. No. Ingredients Name Composition (% w/w) Function
1 Glycerol 48 Solvent
2 Triethanolamine Lauryl Sulfate (TLS) 21.88 Surfactant
3 Sodium Lauryl Sulfate (SLS) 12 Surfactant
4 Hydroxypropyl Methylcellulose (HPMC) 10 Thickening agent
5 Fragrance(LEMON DW-170910) 4 Fragrance
6 Polyvinylpyrrolidone-iodine(PVP-I) 2 Anti-microbial Agent
7 Color Violet 2 Color
8 Neem Extract 0.08 Natural Disinfectant
9 Tetrasodium EDTA 0.04 Preservative
Total 100

1.2 Toilet cleaner concentrate composition B
[0078] A composition for toilet cleaning was prepared based on Table No. 2 below. The process of preparing was same as provided in Example 1.1.
Table No. 2: Toilet Cleaner Concentrate Composition

S. No. Ingredients Name Composition (% w/w) Function
1 Glycerol 48 Solvent
2 CDEA 21.88 Surfactant
3 CAPB 12 Surfactant
4 Hydroxypropyl Methylcellulose (HPMC) 10 Thickening agent
5 Fragrance(LEMON DW-170910) 4 Fragrance
6 Polyvinylpyrrolidone-iodine(PVP-I) 2 Anti-mircobial Agent
7 Color Violet 2 Color
8 Neem Extract 0.08 Antimicrobial Agent
9 Tetrasodium EDTA 0.04 Preservative
Total 100

Example 2
Anti-microbial activity of the composition
[0079] The composition of Example 1 was tested for its anti-microbial activity by a time kill assay. The assay notes the changes in the microbial population within a specified time in presence of the composition. The time kill assays were performed on the ready-to-use formulation. The ready-to-use formulations were prepared after dilution of 25ml of the concentrate composition of EXAMPLE 1.1 in 500 mL of water. The assays were performed as per the American Standard for Testing and Material protocol ASTN-E2315-16.
[0080] Method:
[0081] TEST PROCEDURE- The working cultures of the test organisms were prepared. 10 mL of the sample was taken with respect to individual test culture.100 µL of adjusted test cultures were inoculated in individual samples prepared. A contact time of 20-60 seconds was considered. Using the pour plate technique 1 mL of the formulation was pipetted to triplicated petri dishes for each dilution prepared by serial dilution technique and then 20mL of melted SCDA was added. The petri dish was incubated at 30-35°C for 72 hours. The colonies on the petri dishes were counted with the help of colony counter after incubation and the observations were recorded in cfu (colony forming unit).
[0082] Note- The petri dish showing a number of colonies included in a 15-300 for bacterial and fungal of 15-150 range to be used to perform the result calculation.
LOG REDUCTION CALCULATION
[0083] The effectiveness of the disinfection procedure against each challenge organism was calculated for each product as a log10 reduction as follows:
Log10 Reduction per product = Log10 [Positive Control Count] – Log10 [Test Surface Count]
Note: The log reduction was rounded to the nearest 0.1 value.
[0084] For each product concentration and each experimental condition, calculated and recorded the decimal log reduction separately.
ACCEPTANCE CRITERIA
[0085] In case of surface challenge test positive control should yield 50 to 200% recovery of the microorganism in comparison to inoculums added. Bactericidal, fungicidal or yeasticidal activity on surfaces for general purposes is characterized by the concentration of the tested product. Agents should demonstrate a 3 log reduction of vegetative test organisms and mold spores.
[0086] Results: The results of the test are provided in Table No. 3 below.
Table No. 3: Anti-microbial reduction assessed by time kill assay
S. No. Organism Contact time
(sec) % reduction
1 Candida L albicans(MTCC 227) 20 99.99%
30 99.99%
60 99.99%
2 S. aureus (ATCC 6538) 20 99.99%
30 99.99%
60 99.99%
3 Staph. Epidermis (ATCC12228) 20 99.99%
30 99.99%
60 99.99%
4 Escherichia coli (ATCC 8739) 20 99.99%
30 99.99%
60 99.99%
5 Salmonella abony (NCTC6017) 20 99.99%
30 99.99%
60 99.99%
6 Aspergillus brasilensis (ATCC16404) 20 99.99%
30 99.99%
60 99.99%
7 Lisleria monocytogenes (ATCC 19111) 20 99.99%
30 99.99%
60 99.99%

[0087] As is evident from the above table the composition of the present disclosure is effective in reducing microbial presence for a broad spectrum of microbes. The composition was noted to give superior activity even in a short duration of contact time, i.e., 20 seconds.
Example 3
Solubility of the composition
[0088] The composition of Example 1 was tested for its solubility. The toilet cleaner concentrate composition’s solubility is highly dependent on the solvent glycerol and thickening agent hydroxypropylmethyl cellulose as shown in Table No. 4. Results show that the highest solubility and the desired viscosity 350 cps-400 cps was achieved only at the following values of thickening agent and solvent. At this ratio, composition was stable and there was no precipitation or separation of liquid.
Table No. 4: Variation in solubility of the composition
S. No. Ingredients Name Composition (% w/w)
Solubility
1 Glycerol 48 High Solubility
Hydroxypropyl Methylcellulose (HPMC) 10
2 Glycerol 40
10 Low solubility
Hydroxypropyl Methylcellulose (HPMC)
3 Glycerol 48 Low solubility
Hydroxypropyl Methylcellulose (HPMC) 15
4 Glycerol 30 Low solubility
Hydroxypropyl Methylcellulose (HPMC) 10

ADVANTAGES OF THE PRESENT INVENTION
[0089] The present disclosure provides a toilet cleaner concentrate composition that has quick anti-microbial action.
[0090] The present disclosure provides a toilet cleaner concentrate composition that is free of harsh chemicals such as acids, phenyl, or parabens.
[0091] The present disclosure provides a composition to keep toilets clean, odor-free and microbes free.

CLAIMS:1. A toilet cleaner concentrate composition comprising a natural disinfectant, an anti-microbial agent, a surfactant, hydroxypropyl methylcellulose as a thickening agent, and glycerol as a solvent; wherein the hydroxypropyl methylcellulose is present in a range of 8% w/w to 15% w/w of the composition and wherein the glycerol is present in a range of 45% w/w to 55% w/w of the composition.
2. The composition as claimed in claim 1, wherein the natural disinfectant is selected from neem oil, neem extract, lavender oil, lemon oil, lemon extract, vinegar, tea tree oil, peppermint oil, eucalyptus oil, tulsi extract, orange extract, or combinations thereof.
3. The composition as claimed in claim 1, wherein the anti-microbial agent is selected from polyvinylpyrrolidone-iodine, chlorhexidine, ammonium compound, or iodine compound.
4. The composition as claimed in claim 1, wherein the surfactant is selected from sodium stearate, sodium coco sulfate, coco glucoside, decyl glucoside, sodium lauryl sulfate, ammonium lauryl sulfate, ammonium laureth sulfate, cocoamphocarboxy glycinate, sodium lauryl sulfoacetate, sodium pareth sulfate, sodium myreth sulfate, alpha olefin sulfonate, sodium lauroyl sarcosinate, polysorbate 20, polysorbate 80, triethanolamine lauryl sulphate, cocamide DEA, cocamidopropyl betaine or combinations thereof.
5. The composition as claimed in claim 1, wherein the natural disinfectant is present in a range from 0.5%w/w to 12%w/w of the composition.
6. The composition as claimed in claim 1, wherein the anti-microbial agent is present in a range from 0.5%w/w to 12%w/w of the composition.
7. The composition as claimed in claim 1, wherein the surfactant is present in a range from 0.5%w/w to 25%w/w of the composition.
8. The composition as claimed in claim 1, wherein the composition further comprises one or more additive(s).
9. A toilet cleaner concentrate formulation comprising a natural disinfectant, an anti-microbial agent, a surfactant, hydroxypropyl methylcellulose as a thickening agent, glycerol as a solvent, and one or more additive(s); wherein the hydroxypropyl methylcellulose is present in a range of 8% w/w to 15% w/w of the formulation and wherein the glycerol is present in a range of 45% w/w to 55% w/w of the formulation.
10. A process for preparing a toilet cleaner concentrate composition, wherein the process comprises the steps of: (a) mixing liquid components of the composition in a solvent till homogenous to give a pre-mixture A; (b) grinding and mixing solid components of the composition till homogenous to give pre-mixture B; and (c) blending the pre-mixture A and pre-mixture B and stirring till uniform homogenous composition is obtained; wherein the composition comprises a natural disinfectant, an anti-microbial agent, a surfactant, hydroxypropyl methylcellulose as a thickening agent, and glycerol as a solvent; wherein the hydroxypropyl methylcellulose is present in a range of 8% w/w to 15% w/w of the composition and wherein the glycerol is present in a range of 45% w/w to 55% w/w of the composition.