TITLE OF THE INVENTION
INDUSTRiy^L MANUFACTURING PROCESS FOR DI-HYDROXY-4CETONE VIA MICROBIAL FERMENTATION BY E. COLI.
DESCRIPTION OF THE INVENTION
TECHNICAL FIELD
Dermatologi^als and Cosmoceuticals
BACKGROUND AND NEED OF THE INVENTION
Designed bi(j)-transformation of one chemical entity to another has been pifactices for over 40 years. Producing DIHydroxy Acetone through chemical processes is technically possible, but it involves several stages, each of which is inefficient and target recovery is (ow. Bio-conversion approaches through designer microbial agents (particularly E. Coli) use less expensive substrates (such as fermented complex sugars), inorganic buffers and; under precisely controlled aerobic conditions to produce DIHydroxy Acetone. Our approach and preliminary yields are mjsderate to good under standard industrial scales.

DESCRIPTION OF THE INVENTION
Glycerol (apd its simple derivatives) is efficiently bio-transformed I to DiHydroxy Acetone by designer E. Coli under aerobic condjitions in a medium consisting of yeast hydrolysates inorganic salts and inorganic buffers. The target product is isolated frojm the broth by filtration, concentration and lyophilizatiori.
SUMMARY ^ND DESCRIPTION OF THE INVENTION
The inventioh relates to a novel process for producing DiHydroxy Acetone by designer bio-transformation microbial agents, such as E. Coli. i Specifically, this relates to the conversion, under controlled aerobic conditions, of glycerols and related alcohols to DHA by specjific strains of E. Coli.

What is claimed is:
1. A process for the layer-by-layer moldless production of three-dimensional
shaped products, using powder, by selective melting of regions of the
respective powder layer via input of electromagnetic energy,
which comprises
a process in which the powder comprises at least one polymer powder or copolymer powder produced from a dispersion which comprises at least one polymer or copolymer and which comprises a water-soluble component, which in turn comprises at least one oligosaccharide.
2. The process as claimed in claim 1,
wherein,
the polymer or copolymer has been produced via polymerization, polycondensation, polyaddition, or from natural substances.
3. The process as claimed in any of the preceding claims,
wherein,
the polymer or copolymer comprises a thermoplastic, a thermoset, an elastomer, or a combination thereof.
4. The process as claimed in any of the preceding claims,
wherein,
the polymer or copolymer is composed at least of one unit from the group of the polyesters, copolyesters, polyamides, and copolyamides.
5. The process as claimed in any of the preceding claims,
wherein,
the polymer or copolymer is composed at least of one unit from the group of the polysulfones, polyaryl ether ether ketones, and polyimide.

6. The process as claimed in any of the preceding claims,
wherein,
the polymer or copolymer is composed at least of one unit from the group of polycarbonate, PMMA, and PMMI.
7. The process as claimed in any of the preceding claims,
wherein,
the ratio by weight of the polymer component and of the water-soluble auxiliary component in the dispersion is from 1:99 to 35:60.
8. The process as claimed in any of the preceding claims,
wherein,
the BET surface area to DIN ISO 9277 of the polymer powder used is smaller than or equal to 10 m^/g.
9. The process as claimed in any of the preceding claims,
wherein,
the BET surface area to DIN ISO 9277 of the polymer powder used is smaller than or equal to 3 m^/g.
10. The process as claimed in any of the preceding claims,
wherein,
the BET surface area to DIN ISO 9277 of the polymer powder used is smaller than or equal to 1 m^/g.
11. The process as claimed in any of the preceding claims,
wherein,
the median grain diameter of the polymer powder used is from 10 to 120 |im.
12. The process as claimed in any of the preceding claims,
wherein,
the median grain diameter of the polymer powder used is from 35 to 100 jam.

13. The process as claimed in any of the preceding claims,
wherein,
the median grain diameter of the polymer powder used is from 40 to 70 f^m.
14. The process as claimed in any of the preceding claims,
wherein,
the bulk density to DIN 53466 of the polymer powder used is from 300 to 600 g/l.
15. The process as claimed in any of the preceding claims,
wherein,
the d90:d10 grain size distribution of the polymer powder used is from 3:1 to 15:1.
16. The process as claimed in any of the preceding claims,
wherein,
the polymer powder used comprises auxiliaries and/or fillers.
20. The process as claimed in any of the preceding claims,
wherein,
the polymer powder used comprises powder-flow aids.
21. The process as claimed in any of the preceding claims,
which
comprises inorganic particles as filler.
22. The process as claimed in any of the preceding claims,
which
comprises organic and/or inorganic pigments.
23. The process as claimed in any of the preceding claims,
which
comprises carbon black.

24. The process as claimed in any of the preceding claims,
which
comprises titanium dioxide.
25. A shaped product, produced via one of the processes from the preceding
claims.