Claims:1. A method of synthesizing vitamin D from biofortified raw material, comprises:
taking a raw material (101), wherein the raw material is dried edible mushrooms;
crushing the raw material to obtain a powder (102);
carrying out an extraction of the raw material (103), wherein the extraction is carried out by circulating the raw material and an aqueous solution of ethanol in a ratio to an extractor at a temperature for atleast three hours, wherein the extraction is carried out for atleast two times and filtrates are combined;
concentrating the filtrates (104);
adding the filtrates in water to obtain a solution of precipitates (105);
allowing the precipitates to settle down overnight (106);
filtering the solution and collecting a residue (107);
drying the residue to obtain a powder (108);
milling the powder (109);
blending the powder for an hour (110);
sifting the powder (111); and
packing the powder (112).
2. The method as claimed in claim 1, wherein the ratio of the raw material and the aqueous solution of ethanol is 1:3.
3. The method as claimed in claim 1, wherein the drying is done at 50°C to 60°C and at a vacuum of 500mmHg to 600mmHg.
4. The method as claimed in claim 1, wherein the sifting is done through a 40 size mesh or sieve.
5. The method as claimed in claim 1, wherein the extraction is carried out at a temperature in the range of 70°C to 80°C.
, Description:A) TECHNICAL FIELD OF INVENTION
[001] The present invention generally relates to multivitamin and mineral supplement composition and particularly to a method of synthesizing enriched vitamins and minerals from biofortified raw materials. The present invention more particularly relates to a composition and a method of synthesizing natural vitamins Band enriched using agronomic biofortified crops for use in intestinal inflammation associated with dysbiosis of the gut microbiota.

B) BACKGROUND OF INVENTION
[002] The gut microbiota maintains a symbiotic relationship with the host and regulates several important functions including host metabolism, immunity, and intestinal barrier function. Intestinal inflammation is commonly associated with dysbiosis of the gut microbiota. Alterations in the gut microbiota and associated changes in metabolites as well as disruptions in the intestinal barrier are evidence of the relationship between the gut microbiota and intestinal inflammation. Recent studies have found that many factors may alter the gut microbiota, with the effects of diet being commonly studied.
[003] There are number of ways wherein an unhealthy gut might manifest itself. Some of the most common signs are upset stomach, high sugar diet, unintentional weight changes, sleep disturbances and constant fatigue, skin irritation, autoimmune conditions, food intolerances, etc. Diet is one of many important factors which can affect the gut microbiota and many other aspects of human health and disease. The gut microbiota uses ingested nutrients as an energy source in fundamental biological processes, and changes in diet type or pattern may change the composition of the gut microbiota, as species more suited to metabolizing novel diet types will increase in population, while other species may become less abundance. These changes in the composition of the gut microbiota may affect host physiology and disease resistance.
[004] Fortification is the practice of deliberately increasing the content of an essential micronutrient, i.e. vitamins and minerals (including trace elements) in a food, so as to improve the nutritional quality of the food supply and provide a public health benefit with minimal risk to health.Biofortification is the process by which the nutritional quality of food crops is improved through agronomic practices, conventional plant breeding, or modern biotechnology. Biofortification differs from conventional fortification in that biofortification aims to increase nutrient levels in crops during plant growth rather than through manual means during processing of the crops. Biofortification may therefore present a way to reach populations where supplementation and conventional fortification activities may be difficult to implement and/or limited.
[005] The agronomical techniques include the application of various abiotic elicitors, precursor molecules, pathway intermediates, zinc containing fertilizers, use of soil amendments like lime, gypsum, organic amendments like fym, application of bio-fertilizers like rhizobium (for legumes), azotobacter and azospirillum (non-leguminous crops) and azolla (water fern). Biofortification of curry leaves, holy basil leaves, guava fruits, guava leaves, annato seeds, lemon fruits, amla fruits, Sesbania grandiflora, and bamboo shoots are being employed commercially.
[006] However, there is still a need for a nutritional composition that is capable of promoting a well-balanced intestinal microbiota. There is a need to develop a composition and a method of synthesizing a composition or products that are enriched with essential vitamins and minerals which promote the growth of the gut microbiota and promote or restore an optimal intestinal microbiota ecosystem in an individual beneficial for it. There is a need to come up with a method of synthesizing enriched vitamin B from biofortified raw materials.
[007] The value additions and above mentioned shortcomings, disadvantages and problems are addressed herein, as detailed below.

C) OBJECT OF INVENTION
[008] The primary object of the present invention is to provide a composition of vitamin B to be taken as supplement and a method of synthesizing the composition of said vitamin B supplement from biofortified raw materials.
[009] Another object ofthe present invention is to provide a multivitamin and mineral composition used to promote the growth of the gut microbiota and to promote or restore an optimal intestinal microbiota ecosystem in an individual beneficial for it.
[0010] Yet another object of the present invention is to provide a multivitamin and mineral composition which is in the form of a powder.
[0011] Yet another object of the present invention is to provide a novel method of synthesizing the multivitamin and mineral composition, wherein the vitamin is vitamin B.
[0012] These and other objects and advantages of the embodiments herein will become readily apparent from the following detailed description taken in conjunction with the accompanying drawings.

D) SUMMARY OF INVENTION
[0013] The embodiments of the present invention provide amethod of synthesizing vitamin B supplemental composition. The method comprisesmixing guava leaves extract powder, mango bark extract powder and dried lemon peel extract powder to obtain a mixture (101). The mixture is milled and blended (102). The mixture is sifted through a 40 mesh sifter (103). The mixture is packed in an air tight container (104).
[0014] According to an embodiment of the present invention, the guava leaves extract powder is obtained by a process (1).
[0015] According to an embodiment of the present invention,the mango bark extract powder is obtained by a process (2).
[0016] According to an embodiment of the present invention,the dried lemon peel extract powder is obtained by a process (3).
[0017] According to an embodiment of the present invention,the process (1) comprises the steps of:
(a) taking a raw material, wherein the raw material is bio-fortified dried guava leaves;
(b) crushing the dried leaves to a size in the range of 8 mm – 10 mm;
(c) carrying out an extraction of the raw material, wherein the extraction is carried out by circulating a water added in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water, wherein the extraction is carried out for atleast two times and filtrates are combined;
(d) carrying out membrane filtration of the filtrates, wherein the membrane filtration is carried out by passing the filtrates through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
(e) concentrating the supernatant;
(f) passing the filtrate through a column packed with ion exchange resin (Amberlite);
(g) eluting the column with acidic water (5% organic lemon juice in water) and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
(h) spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0018] According to an embodiment of the present invention,the process (2) comprises the steps of:
(a) taking a raw material, wherein the raw material is bio-fortified dried mango bark;
(b) crushing the raw material to a size in the range of 8mm - 10mm;
(c) carrying out an extraction of the raw material, wherein the extraction is carried out by circulating a water in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water, wherein the extraction is carried out for atleast two times and filtrates are combined;
(d) carrying out membrane filtration of the filtrates, wherein the membrane filtration carried out by passing the filtrates through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
(e) passing the filtrate through a column packed with ion exchange resin (Amberlite);
(f) eluting the column with acidic water (5% organic lemon juice in water) and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%; and
(g) spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0019] According to an embodiment of the present invention,the process (3) comprises the steps of:
(a) taking a raw material, wherein the raw material is bio-fortified dried lemon peel;
(b) crushing the dried lemon peel to a size in the range of 8mm - 10mm using a hammer mill;
(c) carrying out an extraction of the raw material, wherein the extraction is carried out by circulating a water added in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water, wherein the extraction is carried out for atleast two times and filtrates are combined;
(d) carrying out membrane filtration of the filtrates, wherein the membrane filtration carried out by passing the filtrates through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
(e) passing the filtrate through a column packed with ion exchange resin (Amberlite);
(f) eluting the column with acidic water (5% organic lemon juice in water) and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
(g) spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0020] According to another embodiment of the present invention, a vitamin B composition is provided. The vitamin B composition comprises a guava leaves extract powder; a mango bark extract powder, and a dried lemon peel extract powder in a ratio of 1:1:1.
[0021] According to an embodiment of the present invention, the composition is in the powdered form.
[0022] According to an embodiment of the present invention, the guava leaves extract powder, the mango bark extract powder and the dried lemon peel extract powder are added in a ratio of 1:1:1.
[0023] These and other aspects of the embodiments herein will be better appreciated and understood when considered in conjunction with the following description and the accompanying drawings. It should be understood, however, that the following descriptions, while indicating preferred embodiments and numerous specific details thereof, are given by way of illustration and not of limitation. Many changes and modifications may be made within the scope of the embodiments herein without departing from the spirit thereof, and the embodiments herein include all such modifications.

E) BRIEF DESCRIPTION OF DRAWINGS
[0024] The other objects, features and advantages will occur to those skilled in the art from the following description of the preferred embodiment and the accompanying drawings in which:
[0025] FIG. 1 is a flowchart showing the steps involved in the method of synthesizing vitamin B supplemental composition, according to an embodiment of the present invention.
[0026] FIG. 2 is a schematic showing the synthesis of vitamin B supplemental composition, according to an embodiment of the present invention.

F) DETAILED DESCRIPTION OF DRAWINGS
[0027] In the following detailed description, a reference is made to the accompanying drawings that form a part hereof, and in which the specific embodiments that may be practiced is shown by way of illustration. The embodiments are described in sufficient detail to enable those skilled in the art to practice the embodiments and it is to be understood that the logical, mechanical, electronic and other changes may be made without departing from the scope of the embodiments. The following detailed description is therefore not to be taken in a limiting sense.
[0028] The various embodiments of the present provide a multivitamin and mineral composition comprising natural vitamins and minerals extracted and enriched using agronomic biofortified crops. The composition is used is situation of intestinal inflammation associated with dysbiosis of the gut microbiota. According to the embodiments of the present invention, a vitamin B supplemental composition is provided. The vitamin B composition is made from biofortified raw materials. The composition is a unique blend of rich extracts of guava leaves, mango bark and lemon peel, which are said to be good source of vitamin B.
[0029] FIG. 1 is a flowchart showing the steps involved in the method of synthesizing vitamin B supplemental composition, according to an embodiment of the present invention. With respect of FIG. 1, a method of synthesizing vitamin B supplemental composition. The method comprises mixing guava leaves extract powder, mango bark extract powder and dried lemon peel extract powder to obtain a mixture (101). The mixture is milled and blended (102). The mixture is sifted through a 40 mesh sifter (103). The mixture is packed in an air tight container (104).
[0030] According to an embodiment of the present invention, the guava leaves extract powder is obtained by a process (1).
[0031] According to an embodiment of the present invention, the mango bark extract powder is obtained by a process (2).
[0032] According to an embodiment of the present invention, the dried lemon peel extract powder is obtained by a process (3).
[0033] According to an embodiment of the present invention, the process (1) comprises the steps of:
(i) taking a raw material, wherein the raw material is bio-fortified dried guava leaves;
(j) crushing the dried leaves to a size in the range of 8 mm – 10 mm;
(k) carrying out an extraction of the raw material, wherein the extraction is carried out by circulating a water added in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water, wherein the extraction is carried out for atleast two times and filtrates are combined;
(l) carrying out membrane filtration of the filtrates, wherein the membrane filtration is carried out by passing the filtrates through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
(m) concentrating the supernatant;
(n) passing the filtrate through a column packed with ion exchange resin (Amberlite);
(o) eluting the column with acidic water (5% organic lemon juice in water) and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
(p) spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0034] According to an embodiment of the present invention, the process (2) comprises the steps of:
(h) taking a raw material, wherein the raw material is bio-fortified dried mango bark;
(i) crushing the raw material to a size in the range of 8mm - 10mm;
(j) carrying out an extraction of the raw material, wherein the extraction is carried out by circulating a water in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water, wherein the extraction is carried out for atleast two times and filtrates are combined;
(k) carrying out membrane filtration of the filtrates, wherein the membrane filtration carried out by passing the filtrates through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
(l) passing the filtrate through a column packed with ion exchange resin (Amberlite);
(m) eluting the column with acidic water (5% organic lemon juice in water) and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%; and
(n) spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0035] According to an embodiment of the present invention, the process (3) comprises the steps of:
(h) taking a raw material, wherein the raw material is bio-fortified dried lemon peel;
(i) crushing the dried lemon peel to a size in the range of 8mm - 10mm using a hammer mill;
(j) carrying out an extraction of the raw material, wherein the extraction is carried out by circulating a water added in a ratio to an extractor at a temperature of 70°C to 80°C for atleast three hours, wherein the ratio is 1:3 of raw material and water, wherein the extraction is carried out for atleast two times and filtrates are combined;
(k) carrying out membrane filtration of the filtrates, wherein the membrane filtration carried out by passing the filtrates through membranes with pore size of 0.03 to 10 microns with feed operating pressure of 100 to 400kPa (15 to 60psi), and a filtrate and supernatant is collected separately;
(l) passing the filtrate through a column packed with ion exchange resin (Amberlite);
(m) eluting the column with acidic water (5% organic lemon juice in water) and collecting the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%;
(n) spray drying the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C to obtain a dried powder.
[0036] According to another embodiment of the present invention, a vitamin B composition is provided. The vitamin B composition comprises a guava leaves extract powder; a mango bark extract powder, and a dried lemon peel extract powder in a ratio of 1:1:1.
[0037] According to an embodiment of the present invention, the composition is in the powdered form.
PROCESS AND PROCEDURE IN DETAIL
[0038] FIG. 2 is a schematic showing the synthesis of vitamin B supplemental composition, according to an embodiment of the present invention. With respect to FIG. 2, the process is as mentioned below:
[0039] Process 1:
[0040] Raw material: Bio-fortified dried guava leaves free from foreign material are selected.
[0041] Crushing: Crush the dried leaves to reduce the size to 8mm - 10mm using hammer mill.
[0042] Extraction: Charge the raw material and water in the ratio 1:3, into the extractor. Raise the temperature to 70°C to 80°C and circulate the water for 3 hours. After 3 hour filter the extract and collect the filtrate.
[0043] Repeat the above extraction step for one more time. Combine both the filtrates.
[0044] Membrane filtration: Pass the filtrates through membrane with pore size 0.03 to 10 microns, with feed operating pressure approximately 100 to 400kPa (15 to 60psi). Collect both the filtrate and supernatant separately. Concentrate the supernatant.
[0045] Column elution/separation: Pass the filtrate through the column packed with ion exchange resin (Amberlite). Elute the column with acidic water (5% organic lemon juice in water). Collect the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%.
[0046] Spray drying: Spray dry the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C. Collect the dried powder.
[0047] Process 2:
[0048] Raw material: Dried mango bark free from foreign material is selected.
[0049] Crushing: Crush the dried mango bark to reduce the size to 8mm - 10mm using hammer mill.
[0050] Extraction: Charge the raw material and water in the ratio 1:3, into the extractor. Raise the temperature to 70°C to 80°C and circulate the water for 3 hours. After 3 hour filter the extract and collect the filtrate.
[0051] Repeat the above extraction step for one more time. Combine both the filtrates.
[0052] Membrane filtration: Pass the filtrates through membrane with pore size 0.03 to 10 microns, with feed operating pressure approximately 100 to 400kPa (15 to 60psi). Collect both the filtrate and supernatant separately. Concentrate the supernatant.
[0053] Column elution/separation: Pass the filtrate through the column packed with ion exchange resin (Amberlite). Elute the column with acidic water (5% organic lemon juice in water). Collect the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%.
[0054] Spray drying:Spray dry the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C. Collect the dried powder.
[0055] Process 3:
[0056] Raw material: Biofortified dried lemon peel free from foreign material is selected.
[0057] Crushing: Crush the dried lemon peel to reduce the size to 8mm - 10mm using hammer mill.
[0058] Extraction: Charge the raw material and water in the ratio 1:3, into the extractor. Raise the temperature to 70°C to 80°C and circulate the water for 3 hours. After 3 hour filter the extract and collect the filtrate.
[0059] Repeat the above extraction step for one more time. Combine both the filtrates.
[0060] Membrane filtration: Pass the filtrates through membrane with pore size 0.03 to 10 microns, with feed operating pressure approximately 100 to 400kPa (15 to 60psi). Collect both the filtrate and supernatant separately. Concentrate the supernatant.
[0061] Column elution/separation: Pass the filtrate through the column packed with ion exchange resin (Amberlite). Elute the column with acidic water (5% organic lemon juice in water). Collect the eluate and concentrate at 70°C to 80°C to obtain TDS of 30 to 40%.
[0062] Spray drying: Spray dry the concentrated supernatant and concentrated filtrate from column elution at inlet temperature 180° to 190°C and outlet temperature 80°C to 100°C. Collect the dried powder.
[0063] The dried powder from process 1, process 2 and process 3 are milled, blended for an hour, sifted through 40mesh and packed in an air tight container.
[0064] According to an embodiment of the present invention, the vitamin B composition synthesized is effective in the treatment of diarrhoea, Irritable Bowel Syndrome (IBS), antibiotic associated diarrhoea (AAD) related to Clostridium difficile, Clostridium difficile associated diarrhoea (CDAD) and recurrent Clostridium difficile infections (RCDI).
[0065] According to an embodiment of the present invention, the dietary supplement further comprises dietary supplemental amounts of one or more compounds selected from vitamins and minerals other than calcium or phosphorus. The supplement is Vitamin B composition.

G) ADVANTAGES OF INVENTION
[0066] The present invention addresses the problem of gut dysbiosis wherein the composition of the present invention assists in promoting a gut lining and tight junction functioning, ultimately leading to associated effects like reduced inflammation in the intestinal lining.
[0067] The vitamins and minerals composition is used for improving the condition of the digestive system by maintaining a healthy microbiota population by mending ulcerated and damaged intestinal epithelial cell surfaces for the microbiota to populate.
[0068] The vitamins and minerals composition supplies the right amount of the right micronutrients at the right time to assure adequate intake of micronutrients needed for disease prevention and protection against nutritional losses and deficiencies due to lifestyle factors and common inadequate dietary patterns.
[0069] It is to be understood that the phraseology or terminology employed herein is for the purpose of description and not of limitation. Therefore, while the embodiments herein have been described in terms of preferred embodiments, those skilled in the art will recognize that the embodiments herein can be practiced with modification within the spirit and scope of the claims presented in the complete specification or non-provisional application.