Description:TECHNICAL FIELD

[0001] The present invention relates to the formulations that can be used as sanitizers, antiseptics, disinfectants and other antimicrobial liquids. This formulation is non-alcoholic, non-toxic and eco-friendly liquid that can kill microbes through contact killing.
BACKGROUND ART
[0002] Microorganisms (or microbes) are single cell, cell cluster, or multicellular microscopic (or macroscopic) organisms including but not limited to, bacteria, fungi, archaea, protists, plants (e.g., green algae), viruses, prions, parasites, and animals (e.g. amoeba, plankton). Microorganisms may be beneficially exploited in a variety of fields, such as biotechnology, food and beverage preparation, and diagnostic technologies. In other contexts, however, short or long-term exposure to microorganisms may be harmful and lead to one or more of a variety of illnesses and diseases in plants, animals, and humans.
[0003] Contamination of surfaces with one or more types of microorganisms, and/or the transfer of microorganisms between surfaces can lead to a variety of negative effects, including but not limited to illness, disease, passage of disease, increased medical care costs, and/or loss of work time and associated loss of revenue. To address such issues and reduce the presence of a broad spectrum of microorganisms from a variety of surfaces.
[0004] Essential oils are volatile oils obtained from plant or animal sources and are composed of complex mixtures of several constituents, such as monoterpenes and sesquiterpene hydrocarbons, monoterpene and sesquiterpene alcohols, esters, ethers, aldehydes, ketones, oxides and the like. These essential oils and their isolated constituents are frequently utilized as fragrance and flavor agents, and have been widely used in folk medicine for wound healing properties.
[0005] It is a standard practice in the industrialized world to disinfect the skin prior to any invasive procedure such as surgery, catheterization, or needle puncture to reduce the risk of infection. Currently, chlorhexidine compositions are an agent of choice for disinfecting hands, skin, surgical sites, catheter sites, and oral cavities. Chlorhexidine and its salts are well-known antimicrobials with excellent efficacy that are safe to use. Chlorhexidine and its salts also show persistent antimicrobial activity on the skin often for more than 24 hours.
[0006] US2017/0280715A1 discloses a hand sanitizing composition and a method of manufacture, wherein the composition includes a solvent from about 78% to about 96% by weight sufficient to operate as a carrier and solvent. The composition includes tetrasodium EDTA and/or Sodium Citrate from about 0.01% to about 1% by weight to operate as a buffer, stabilizer and chelating agent. The composition includes a humectant selected from the group of humectants consisting of: Cetyl Alcohol, Ceteryl Alcohol, and Steryl Alcohol from about 1.5% to about 5.5% by weight to emulsify, thicken and lubricate. The composition includes an emollient from about 0.1% to about 3.2% by weight and an antioxidant vitamin from about 0.01% to about 1% by weight. The composition includes a soothing agent in a therapeutically significant amount and a conditioner.
[0007] US20030008791A1 discloses The present invention discloses a sanitizing composition including (a) a sanitizing effective amount of an antimicrobial agent, (b) a water-soluble silicone, (c) a humectant selected from the group consisting of hydrogenated starch hydrolysates, glycerin, and mixtures thereof, and (d) water. This sanitizing composition is preferably substantially free of alcohol and more preferably contains less than about 0.5 or 1% by weight of alcohol, based upon 100% by weight of total composition. The hand sanitizer of the present invention is non-flammable, disinfects and/or sanitizes surfaces rapidly, and quickly dries after application. Another embodiment is a method of disinfecting and/or sanitizing a surface which comprises applying the aforementioned hand sanitizer composition to the surface. In particular, the hand sanitizer composition may be topically applied to hands, face and other surfaces of the body to disinfect and sanitize the same.
[0008] However, there is a need in the art to provide a formulation which is non-alcoholic, non-toxic and ecofriendly and can overcome the drawbacks of the prior arts.
OBJECTS OF THE INVENTION

[0009] An object of the present invention is to develop a formulation for water-based contact killing formulations to inhibit microbial growth on any surfaces.
[0010] Another object of the present invention is to develop non-alcoholic, non-toxic and ecofriendly liquids that can kill microbes through contact killing.
[0011] Yet another object of the present invention is to develop a formulation with anti-microbial effect which includes Silver or Cooper-Cerium or titanium dioxide or Zinc oxide nano particles as antimicrobial agents.
[0012] Yet another object of the present invention is that it includes natural monoterpenoid phenolic compound, preservative, humectant, stabilizer and fragrance.
[0013] Yet another object of the present invention and these formulations were introduced by non-toxic, non-flammable, non-volatile, non-corrosive strong antimicrobial liquids that can easily dry after application and make a contact killing thin films to protect the surfaces for months to years.
[0014] Yet another object of the present invention discloses that most of the infections are happened by the surface contamination, and the formulation of the present invention has properties to protect the surfaces through microbial contamination by inhibiting the microbial growth, killing immediately and prevent them to grow till 61 days after single application.
[0015] The foregoing and other objects of the present invention will become readily apparent upon further review of the following detailed description of the embodiments as illustrated in the accompanying drawings.
SUMMARY OF THE INVENTION

[0016] The present invention relates to the formulations that can be used as sanitizers, antiseptics, disinfectants and other antimicrobial liquids. This formulation is non-alcoholic, non-toxic and eco-friendly liquid that can kill microbes through contact killing.
[0017] According to an embodiment of the present invention a formulation, comprising: i) Copper-cerium nanoparticles in the range of 0.003 – 0.03% w/w; ii) Isosorbide nitro derivative (0.01-0.1%), iii) Benzalkonium Chloride in the range of 0.03 – 0.3% w/w; iv) Sodium metasilicate in the range of 0.1 -0.5% w/w; v) Sodium benzoate in the range of 0.1 – 0.3% w/w; vi) Oleic acid in the range of 0.01 – 0.03% w/v; vii) Turpentine oil/mint oil in the range of 0.1 –0.3% w/w; viii) Glycerol in the range of 0.1 - 2% w/v; ix) Adipic acid in the range of 0.005 – 0.5% w/v; x) Hydrogen peroxide in the range of 0.1-0.125% w/v; xi) Carbopol 940 in the range of 0.01 -0.5% w/v; xii) Polyvinyl pyrrolidone in the range of 0.001 – 0.2% w/w; and xiii) Water.

[0018] According to another embodiment of the present invention the ingredients disclosed are mixed in the defined concentrations to prepare the formulation.

[0019] While the invention has been described and shown with reference to the preferred embodiment, it will be apparent that variations might be possible that would fall within the scope of the present invention.

BRIEF DESCRIPTION OF DRAWINGS

[0020] So that the manner in which the above-recited features of the present invention may be understood in detail, a more particular description of the invention, briefly summarized above, may have been referred by embodiments, some of which are illustrated in the appended drawings. It is to be noted, however, that the appended drawings illustrate only typical embodiments of this invention and are therefore not to be considered limiting of its scope, for the invention may admit to other equally effective embodiments.
[0021] These and other features, benefits, and advantages of the present invention will become apparent by reference to the following text figure, with like reference numbers referring to like structures across the views, wherein:
[0022] Figure 1 depicts a line graph with results of incubation observations for Experiment 1.
[0023] Figure 2 shows the line graph with the observations of experiment 2.
[0024] Figure 3 illustrates a line graph with the observations of experiment 3.
[0025] Figure 4 and 5 depicts long term contact killing effects of indigenous formulations.
DETAILED DESCRIPTION OF THE INVENTION

[0026] While the present invention is described herein by way of example using embodiments and illustrative drawings, those skilled in the art will recognize that the invention is not limited to the embodiments of drawing or drawings described and are not intended to represent the scale of the various components. Further, some components that may form a part of the invention may not be illustrated in certain figures, for ease of illustration, and such omissions do not limit the embodiments outlined in any way. It should be understood that the drawings and the detailed description thereto are not intended to limit the invention to the particular form disclosed, but on the contrary, the invention is to cover all modifications, equivalents, and alternatives falling within the scope of the present invention as defined by the appended claim.
[0027] As used throughout this description, the word "may" be used in a permissive sense (i.e., meaning having the potential to), rather than the mandatory sense, (i.e., meaning must). Further, the words "a" or "an" mean "at least one” and the word “plurality” means “one or more” unless otherwise mentioned. Furthermore, the terminology and phraseology used herein are solely used for descriptive purposes and should not be construed as limiting in scope. Language such as "including," "comprising," "having," "containing," or "involving," and variations thereof, is intended to be broad and encompass the subject matter listed thereafter, equivalents, and additional subject matter not recited, and is not intended to exclude other additives, components, integers, or steps. Likewise, the term "comprising" is considered synonymous with the terms "including" or "containing" for applicable legal purposes. Any discussion of documents, acts, materials, devices, articles, and the like are included in the specification solely for the purpose of providing a context for the present invention. It is not suggested or represented that any or all these matters form part of the prior art base or were common general knowledge in the field relevant to the present invention.
[0028] In this disclosure, whenever a composition or an element or a group of elements is preceded with the transitional phrase “comprising”, it is understood that we also contemplate the same composition, element, or group of elements with transitional phrases “consisting of”, “consisting”, “selected from the group of consisting of, “including”, or “is” preceding the recitation of the composition, element or group of elements and vice versa.
[0029] The present invention is described hereinafter by various embodiments with reference to the accompanying drawing, wherein reference numerals used in the accompanying drawing correspond to the like elements throughout the description. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiment set forth herein. Rather, the embodiment is provided so that this disclosure will be thorough and complete and will fully convey the scope of the invention to those skilled in the art. In the following detailed description, numeric values and ranges are provided for various aspects of the implementations described. These values and ranges are to be treated as examples only and are not intended to limit the scope of the claims. In addition, several materials are identified as suitable for various facets of the implementations. These materials are to be treated as exemplary and are not intended to limit the scope of the invention.
[0030] The present invention pertains to the formulations that can be used as sanitizers, antiseptics, disinfectants and other antimicrobial liquids. This formulation is non-alcoholic, non-toxic and eco-friendly liquid that can kill microbes through contact killing. The formulation can also be used to break the biofilm.
[0031] According to an embodiment of the present invention a formulation, comprising: i) Copper-cerium nanoparticles in the range of 0.003 – 0.03% w/w; ii) Isosorbide nitro derivative (0.01-0.1%), iii) Benzalkonium Chloride in the range of 0.03 – 0.3% w/w; iv) Sodium metasilicate in the range of 0.1 -0.5% w/w; v) Sodium benzoate in the range of 0.1 – 0.3% w/w; vi) Oleic acid in the range of 0.01 – 0.03% w/v; vii) Turpentine oil/mint oil in the range of 0.1 –0.3% w/w; viii) Glycerol in the range of 0.1 - 2% w/v; ix) Adipic acid in the range of 0.005 – 0.5% w/v; x) Hydrogen peroxide in the range of 0.1-0.125% w/v; xi) Carbopol 940 in the range of 0.01 0.5% w/v; xii) Polyvinyl pyrrolidone in the range of 0.001 – 0.2% w/w; and xiii) Water.

[0032] According to another embodiment of the present invention the ingredients disclosed are mixed in the defined concentrations to prepare the formulation.

[0033] In an aspect the Copper-Cerium nanoparticles in the formulation acts as antimicrobial agents which includes but not limited to Silver, titanium dioxide, Zinc oxide nano-particles, Benzalkonium Chloride, Sodium metasilicate, Sodium benzoate, Oleic acid, Adipic acid, Hydrogen peroxide, Cetrimide, Camphor, menthol, thymol, sorbic acid, coumarin, oxalic acid, benzoic acid, ascorbic acid, boric acid, citric acid, maleic acid , malic acid, picric acid, gallic acid, fumaric acid, linalool, eugenol, salicylic acid, Silver nitrate, caprylic acid, caproic acid, boric acid, benzyl alcohol, ascorbic acid, crotonic acid, itaconic acid and combinations thereof.
[0034] In an aspect, the Turpentine oil/mint oils acts as stabilizers. Carbopol 940 and Polyvinyl pyrrolidone acts as thickeners.
[0035] In an aspect Glycerol acts as humectants but not limited to PEG 200, PEG 400, cellulose and its derivatives, dextrin.
[0036] In an aspect polysorbate(s), Neem oil, sodium lauryl sulphate, Tetraethyl orthosilicate are also used as emulsifying agents.
[0037] TABLE I: The components of formulations have been described in table I. The range of concentration was given in percentage by weight for 20X concentrated formulation.
TABLE I
Components Broad Remarks
Antimicrobial agent 0.0003-0.30% To kill the microorganism.
preservatives 0.01 0.2% To provide stability of solution.
Thickener 0.05 - 0.1% To provide stability.
Humectant 0.01 – 2.45% Avoid to dryness of skin.
Fragrance 0.05-0.1% To provide good aroma.
water Quantity sufficient To maintain volume.

[0038] TABLE II: the concentration ranges of components present in 3000X concentrated formulations has mentioned in table II. The concentrated formulations were shown as pest or semi liquids state.
Components Broad Remarks
Antimicrobial agent 1-5% Deactivate microbes.
preservatives 0.1-2% Provide stability.
Thickener 0.01-1% Provide stability.
Humectant 0.01-1% Beneficial for skin.
Fragrance 0.01-0.1% Good aroma.
Water Quantity sufficient Maintain concentration or as carrier

[0039] The pH ranges of formulations were 4 to 6. 10% sodium hydroxide solution was used to maintained neutral pH. After preparation, solutions were observed clear and transparent. The final formulations were showing foaming characters.
[0040] In an aspect fragrances are also added to the formulation which includes but not limited to Pineapple, Butterscotch, Vanilla, Chocolate, Turpentine oil, mint oil, rose water etc.
[0041] In an aspect the formulation of the present invention is prepared in the form of liquid, pest, powder state.
[0042] EXPERIMENTS
ANTIMICROBIAL TESTING:
[0043] The surfaces of agar media including Nutrient agar media, Mueller Hinton Media and LB (Luria-Bertani) agar media were protected from microbial growth by single application of these formulations.
[0044] The killing time of microbes was inversely proportional to the concentration of solution and directly proportional to the CFU value of microbes. So, the killing time was depended on the concentration of solutions and CFU value of microorganism.
[0045] The 100µL aliquot from stock cultured solutions were mixed with 100µL formulation solutions in 0.5 mL Eppendorf tube than cultured on nutrient agar media. After 18 hours incubation observation was taken. The 1:10 ratio of solutions and aliquot of Escherichia coli strain ATCC25922 in nutrient broth deactivated within 15 seconds.
[0046] The 100µL aliquot from stock cultured solutions were mixed with 10µL solutions in 0.5 mL Eppendorf tube than cultured on nutrient agar media. After 18 hours incubation observation was taken.
[0047] The microbes those were present on the surfaces of toilet sheet can be deactivated immediately after application of formulations.
[0048] The samples were collected from toilet sheet surfaces for negative control. Liquid formulation was spread on the surfaces of toilet sheet. Then immediately and in different time period samples were collected with the help of sterile cotton bud. All samples were cultured on nutrient agar media surfaces under sterile conditions. After 18 hours incubation observation was taken.
[0049] STABILITY TESTING OF FORMULATION
[0050] Referring to Figure 1, after single application of formulations on the surface of nutrient agar media inoculums were given in different time periods, inoculum (Escherichia coli strain ATCC25922) was taken from stock culture solution. After 18 hour’s incubation observations was been taken.
[0051] Experiment II
[0052] Referring to figure 2, the testing of antimicrobial activity of those formulations were done on nutrient agar media, Mueller Hinton media, Luria-Bertani agar media and modified fungal growth promoting media. The observations were shown as described by following line chart II, given below.
[0053] Experiments: Media containing petridishes were prepared in wild condition. After solidify solution was spread on the surfaces of media individually. Experiments were allowed for incubation in wild condition. Observation was taken within time periods.
[0054] After application of these solutions were protects the surfaces from contamination of microorganisms till 61 days.
[0055] Experiment III
[0056] Referring to Figure 3, solutions were spread on individual glass slides surfaces and allowed for dry in wild conditions. Sample were collected from slides and cultured, sample were collected within periods of times. Experiments were allowed for incubation. After 18 hours incubation observation had been taken as shown in following line chart III.
[0057] Figures 4 and 5 refers to long term contact killing effects of indigenous formulations.

[0058] ADVANTAGES and APPLICATIONS
1. These formulations were introduced by non-toxic, non-flammable, non-volatile, non-corrosive strong antimicrobial liquids that can easily dry after application and make a contact killing thin films to protect the surfaces for months to years.
2. These solutions can be applicable on the skin, cloths, furniture’s, glasses, marbles, woods, water proof painted surfaces, biological instruments surfaces, other electronic instruments surfaces and hard surfaces.
3. These formulations can be suitable for sanitization of daily used stuff including gadgets, surgical instrument, personal protective equipment, remotes, chair sheets and also to break biofilm.
4. These formulations can be used in hospitals, public transports, schools’ collages and other public places as disinfectants.
5. Pharmaceutical applications include as Mouth wash, Wound cleaners, Applied on cuts & scratches as antiseptics, Disinfectants
6. These formulations can be applicable for deactivating the antimicrobial resistance microbes.
[0059] Further, the operations need not be performed in the disclosed order, although in some examples, an order may be preferred. Also, not all functions need to be performed to achieve the desired advantages of the disclosed system and method, and therefore not all functions are required.
[0060] Various modifications to these embodiments are apparent to those skilled in the art from the description and the accompanying drawings. The principles associated with the various embodiments described herein may be applied to other embodiments. Therefore, the description is not intended to be limited to the 5 embodiments shown along with the accompanying drawings but is to be providing the broadest scope consistent with the principles and the novel and inventive features disclosed or suggested herein. Accordingly, the invention is anticipated to hold on to all other such alternatives, modifications, and variations that fall within the scope of the present invention and appended claims.
, Claims:I/We Claim:
1) A Water-based contact killing formulation, comprising:
i) Copper-cerium nanoparticles in the range of 0.003 – 0.03% w/w;
ii) Isosorbide nitro derivatives 0.01 – 0.3% w/w,
iii) Benzalkonium Chloride in the range of 0.03 – 0.3% w/w;
iv) Sodium metasilicate in the range of 0.1 -0.5% w/w;
v) Sodium benzoate in the range of 0.1 – 0.3% w/w;
vi) Oleic acid in the range of 0.01 – 0.03% w/v;
vii) Turpentine oil/mint oil in the range of 0.1 –0.3% w/w;
viii) Glycerol in the range of 0.1 - 2% w/v;
ix) Adipic acid in the range of 0.005 – 0.5% w/v;
x) Hydrogen peroxide in the range of 0.1-0.125% w/v;
xi) Carbopol 940 in the range of 0.01 -0.5% w/v;
xii) Polyvinyl pyrrolidone in the range of 0.001 – 0.2% w/w; and
xiii) Water.
2) The formulation as claimed in claim 1, wherein said Copper-Cerium nanoparticles acts as antimicrobial agents which includes but not limited to Silver, titanium dioxide, Zinc oxide nano-particles, Sodium metasilicate, Sodium benzoate, Oleic acid, adipic acid, Hydrogen peroxide cetrimide, camphor, menthol, thymol, sorbic acid, coumarin, oxalic acid, benzoic acid, ascorbic acid, boric acid, citric acid, maleic acid , malic acid, picric acid, gallic acid, fumaric acid, linalool, eugenol, salicylic acid, Silver nitrate, caprylic acid, caproic acid, boric acid, benzyl alcohol, ascorbic acid, crotonic acid, itaconic and combinations thereof.
3) The formulation as claimed in claim 1, wherein said formulation is used to kill acid any kind of microbes through contact killing within nano to few seconds.
4) The formulation as claimed in claim 1, wherein said Carbopol 940 and Polyvinyl pyrrolidone acts as thickeners.
5) The formulation as claimed in claim 1, wherein said Glycerol acts as a humectant but not limited to PEG 200, PEG 400, cellulose, dextrin, natural resins and its derivatives.
6) The formulation as claimed in claim 1, wherein polysorbate(s), Neem oil, sodium lauryl sulphate, Tetraethyl orthosilicate are also used as emulsifying agents.
7) The formulation as claimed in claim 1, wherein fragrances are also added to the formulation which includes but not limited to Pineapple, Butterscotch, Vanilla, Chocolate, Turpentine oil, mint oil, rose water etc.
8) The method for preparation of formulation as claimed in claim 1, wherein said steps in preparing, includes mixing of all the ingredients in varying concentrations with and without metal nanoparticles.
9) The formulation as claimed in claim 1, wherein said formulation can be prepared in the form of liquid, pest, powder state and store it for months to years before using it with normal water.
10) The formulation as claimed in claim 1, wherein said formulation is used as sanitizers, antiseptics, mouth wash, cleansers, to remove bad smell and cloth fresheners in laundry, preservatives, disinfectants, wound washing, removing of germs from skins, personal protective equipment, surgical stuffs, protection against infection from cuts and scratches. The formulation does not change or deform of any object and protect surfaces for months to years after single application. The product can also be used to minimize bad smell and keep hygienic of any kind of toilets without flashing any water.