DESC:FIELD OF THE INVENTION
[0001] The present disclosure relates to an antimicrobial handrub composition. Specifically, the present disclosure provides a waterless antimicrobial handrub composition comprising natural antimicrobial agents, bio-surfactants, natural gums or thickener, natural emollient, and essential oils. The present disclosure also provides a process for preparation of the waterless antimicrobial handrub composition.

BACKGROUND OF THE INVENTION
[0002] Background description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art.
[0003] Personal hygiene and in particular the sanitization of hands is widely accepted as a particularly effective means to hinder or prevent the transmittal of microorganisms by touch. Transmittal of microorganisms by touch poses a significant health hazard, particularly in health care industries, such as hospitals, or in industrial or domestic food preparation. However, only a limited range of antimicrobial compositions are suitable for use on human skin. Such antimicrobial agents may include for example alcohols, trichlorohydroxy diphenyl ether (Tri-closan), and parachlorometaxylenol amongst others which are not skin friendly and may lead to skin irritation. These might even lead to building up of anti-microbial resistance after regular use.
[0004] Furthermore, alcohol based handrubs suffer from various disadvantages. Alcohol based handrubs typically contain very high weight percent alcohol (60-90%), which can remove the protective sebum layer on the skin and dry the user's hands leading to irritation. Consequently, a user may be unwilling to sanitize their hands as often as is necessary to prevent health risk. Additionally, typical alcohol based handrubs kill only a few type of microorganisms and spores. Alcohol based handrubs also pose a significant fire hazard if stored in large quantities.
[0005] Few non-alcohol based handrubs are available but these have been unable to replace rival alcoholic handrubs because they are not as effective against a range of microorganisms.
[0006] Therefore, there exists a need for an alcohol-free handrub composition suitable for use on human skin which is both effective and fast acting against a wide range of microorganisms, and evaporates from and/or is absorbed into the skin without the need for a water rinse. The present disclosure provides a waterless antimicrobial handrub which is not only efficacious but also moisturizes and maintains the health of the skin.

OBJECTS OF THE INVENTION
[0007] An object of the present disclosure is to provide a waterless antimicrobial handrub composition that satisfies the existing needs, as well as emerging ones, and generally overcomes the deficiencies found in the prior art.
[0008] Another object of the present disclosure is to provide a waterless antimicrobial handrub composition that has broad spectrum antimicrobial properties i.e., against a range of bacteria, bacterial spores and fungi with up to 99.9% effectiveness.
[0009] Another object of the present disclosure is to provide a waterless antimicrobial handrub composition that is effective against methicillin-resistant Staphylococcus aureus.
[0010] Another object of the present disclosure is to provide a waterless antimicrobial handrub composition that is free from harsh chemicals like alcohol, benzalkonium chloride compounds, parabens or sodium lauryl sulphate (SLS).
[0011] Another object of the present disclosure is to provide a waterless antimicrobial handrub composition that moisturizes the skin and keeps it soft.

SUMMARY OF THE INVENTION
[0012] This summary is provided to introduce a selection of concepts in a simplified form that are further described below in Detailed Description section. This summary is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used as an aid in determining the scope of the claimed subject matter.
[0013] The present disclosure relates to a handrub composition. Specifically, the present disclosure provides a handrub composition having moisturizing as well as anti-microbial properties.
[0014] In an aspect, the present disclosure provides a waterless antimicrobial handrub composition comprising natural antimicrobial agents, bio-surfactants, natural gums or thickener, natural emollient and essential oils.
[0015] In an embodiment, the antimicrobial agents may be selected from povidone iodine (PVP-I), chlorhexidine digluconate, octenidine dihydrochloride, citric acid, or combinations thereof.
[0016] In an embodiment, the bio-surfactants may be selected from rhamnolipid, sophorolipid, trehalolipids, mannosylerythritol lipids, surfactin, viscosin, ornithine or combinations thereof.
[0017] In an embodiment, the natural gum or thickener may be selected from guar gum, methyl cellulose, xanthan gum, gum arabic, gum ghatti, locust bean gum, carbopol, cornstarch, gelatin or combinations thereof.
[0018] In an embodiment, the natural emollient may be selected from castor oil, jojoba oil, squalene, cocoa butter, shea butter, or combinations thereof.
[0019] In an embodiment, the essential oils may be selected from clove oil, oregano oil, tea tree oil, thyme oil, cinnamon oil, neem oil, lemongrass oil, lavender oil, or combinations thereof.
[0020] In an embodiment, the composition may further comprise one or more additive(s). The additive may be selected from moisturizer, emulsifier, thickener, preservative, pH regulator, coloring agent, fragrance, or combinations thereof.
[0021] In a preferred embodiment, the composition may comprise of sophorolipid, povidone iodine; jojoba oil or castor oil; neem oil or tea tree oil; guar gum or methyl cellulose; lecithin; sodium hydroxide; and citric acid.
[0022] In an aspect, the present disclosure provides a process of preparing a waterless antimicrobial handrub composition, wherein the process comprises mixing natural antimicrobial agents, bio-surfactants, natural gums or thickener, natural emollient, essential oils, and optionally one or more additive(s).
[0023] Various objects, features, aspects and advantages of the inventive subject matter will become more apparent from the following detailed description of preferred embodiments.

DETAILED DESCRIPTION
[0024] Unless the context requires otherwise, throughout the specification and claims which follow, the word “comprise” and variations thereof, such as, “comprises” and “comprising” are to be construed in an open, inclusive sense that is as “including, but not limited to.”
[0025] Reference throughout this specification to “one embodiment” or “an embodiment” means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrases “in one embodiment” or “in an embodiment” in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
[0026] As used in this specification and the appended claims, the singular forms “a,” “an,” and “the” include plural referents unless the content clearly dictates otherwise. It should also be noted that the term “or” is generally employed in its sense including “and/or” unless the content clearly dictates otherwise.
[0027] In some embodiments, the numbers expressing quantities of ingredients, properties such as concentration, reaction conditions, and so forth, used to describe and claim certain embodiments of the invention are to be understood as being modified in some instances by the term “about.” Accordingly, in some embodiments, the numerical parameters set forth in the written description are approximations that can vary depending upon the desired properties sought to be obtained by a particular embodiment. In some embodiments, the numerical parameters should be construed in light of the number of reported significant digits and by applying ordinary rounding techniques. Notwithstanding that the numerical ranges and parameters setting forth the broad scope of some embodiments of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as practicable.
[0028] The recitation of ranges of values herein is merely intended to serve as a shorthand method of referring individually to each separate value falling within the range. Unless otherwise indicated herein, each individual value is incorporated into the specification as if it were individually recited herein. All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context. The use of any and all examples, or exemplary language (e.g. “such as”) provided with respect to certain embodiments herein is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention otherwise claimed. No language in the specification should be construed as indicating any non-claimed element essential to the practice of the invention.
[0029] The headings and abstract of the invention provided herein are for convenience only and do not interpret the scope or meaning of the embodiments.
[0030] The following discussion provides many example embodiments of the inventive subject matter. Although each embodiment represents a single combination of inventive elements, the inventive subject matter is considered to include all possible combinations of the disclosed elements. Thus if one embodiment comprises elements A, B, and C, and a second embodiment comprises elements B and D, then the inventive subject matter is also considered to include other remaining combinations of A, B, C, or D, even if not explicitly disclosed.
[0031] The term “waterless antimicrobial handrub composition” as used herein throughout the present disclosure denotes the handrub or cleansing compositions that do not require utilization of water or require minimal amount of water (such as use of wet cloth or sponge or wipe) to effect removal of dirt or soil along with the composition.
[0032] The present disclosure relates to a waterless antimicrobial handrub composition. Specifically, the present disclosure provides a waterless antimicrobial handrub composition that has antimicrobial effect but also protects and moisturizes the skin.
[0033] In an embodiment, the present disclosure provides a waterless antimicrobial handrub composition comprising natural antimicrobial agents, bio-surfactants, natural gums or thickener, natural emollient and essential oils.
[0034] In an embodiment, the antimicrobial agents may be selected from povidone iodine (PVP-I), chlorhexidine digluconate, octenidine dihydrochloride, citric acid, or combinations thereof.
[0035] In an embodiment, the anti-microbial agent may be present in an amount ranging from about 0.1% w/w to about 10% w/w, preferably in the amount ranging from about 1% w/w to about 7.5% w/w of the composition.
[0036] In an embodiment, the bio-surfactants may be selected from rhamnolipid, sophorolipid, trehalolipids, mannosylerythritol lipids, surfactin, viscosin, ornithine or combinations thereof.
[0037] In an embodiment, the bio-surfactant may be present in an amount ranging from about 0.001% w/w to about 5% w/w, preferably in the amount ranging from about 0.01% w/w to about 1% w/w of the composition.
[0038] In an embodiment, the natural gum or thickener may be selected from guar gum, methyl cellulose, xanthan gum, gum arabic, gum ghatti, locust bean gum, carbopol, cornstarch, gelatin or combinations thereof.
[0039] In an embodiment, the natural gum or thickener may be present in an amount ranging from about 0.1% w/w to about 20% w/w, preferably in the amount ranging from about 1% w/w to about 10% w/w of the composition.
[0040] In an embodiment, the natural emollient may be selected from castor oil, jojoba oil, squalene, cocoa butter, shea butter, or combinations thereof.
[0041] In an embodiment, the natural emollient may be present in an amount ranging from about 0.05% w/w to about 10% w/w, preferably in the amount ranging from about 0.5% w/w to about 5% w/w of the composition.
[0042] In an embodiment, the essential oils may be selected from clove oil, oregano oil, tea tree oil, thyme oil, cinnamon oil, neem oil, lemongrass oil, lavender oil, or combinations thereof.
[0043] In an embodiment, the essential oils may be present in an amount ranging from about 0.05% w/w to about 10% w/w, preferably in the amount ranging from about 0.5% w/w to about 5% w/w of the composition.
[0044] In an embodiment, the composition may further comprise one or more additive(s). The additive may be selected from moisturizer, emulsifier, thickener, preservative, pH regulator, coloring agent, fragrance, or combinations thereof. However, a person of skill in the art would understand that any other additive(s) well-known in the art may be employed without going beyond the spirit and scope of the present disclosure.
[0045] In an embodiment, the additives may be present in an amount ranging from about 0.1% w/w to about 1.0% w/w of the composition.
[0046] In an embodiment, the moisturizer may be selected from vegetable glycerin, shea butter, cocoa butter, argan oil, cucumber extract, aloe vera, olive oil, sunflower oil, avocado oil, jojoba oil, or combinations thereof. However, a person of skill in the art would understand that any other moisturizer(s) well-known in the art may be employed without going beyond the spirit and scope of the present disclosure.
[0047] In an embodiment, the emulsifier may be selected from lecithin, coco-glucoside, beeswax, candelilla wax, shea, butter, cocoa butter, or combinations thereof. However, a person of skill in the art would understand that any other emulsifier(s) well-known in the art may be employed without going beyond the spirit and scope of the present disclosure.
[0048] In an embodiment, the emulsifier may be present in an amount ranging from about 0.1% w/w to about 20% w/w, preferably in the amount ranging from about 1% w/w to about 10% w/w of the composition.
[0049] In an embodiment, the preservative may be selected from citric acid, benzoic acid, sorbic acid, triclosan, triclocarban, phenoxyethanol, benzyl benzoate, benzyl alcohol, zinc pyrithione, benzalkonium chloride, or combinations thereof. However, a person of skill in the art would understand that any other preservative(s) well-known in the art may be employed without going beyond the spirit and scope of the present disclosure.
[0050] In an embodiment, the pH regulator may be selected from sodium hydroxide, citric acid, lactic acid, triethanolamine, glacial acetic acid, sodium carbonate, acetic acid, or combinations thereof. However, a person of skill in the art would understand that any other pH regulator(s) well-known in the art may be employed without going beyond the spirit and scope of the present disclosure.
[0051] In an embodiment, the pH regulator may be present in an amount ranging from about 0.001% w/w to about 5% w/w, preferably in the amount ranging from about 0.01% w/w to about 1% w/w of the composition.
[0052] In an embodiment, the coloring agent may be selected from blue coloring, yellow coloring, orange coloring, pink coloring, blue coloring, green coloring, or combinations thereof. However, a person of skill in the art would understand that any other coloring agent(s) well-known in the art may be employed without going beyond the spirit and scope of the present disclosure.
[0053] In an embodiment, the fragrance may be selected from mint, lemon, rose, citrus, orange, lemongrass, lavender or combinations thereof. However, a person of skill in the art would understand that any other fragrance(s) well-known in the art may be employed without going beyond the spirit and scope of the present disclosure.
[0054] In an embodiment, the fragrance may be present in an amount ranging from about 0.01% w/w to about 5% w/w, preferably in the amount ranging from about 0.1% w/w to about 3% w/w of the composition.
[0055] In an embodiment, the composition may be based in a solvent including water, distilled water, glycerol, purified water, or combinations thereof.
[0056] In a preferred embodiment, the composition may comprise of sophorolipid; povidone iodine; jojoba oil or castor oil; neem oil or tea tree oil; guar gum or methyl cellulose; lecithin; sodium hydroxide; water and citric acid.
[0057] In a more preferred embodiment, the composition may comprise of sophorolipid in about 0.01% to about 1%; povidone iodine in about 1% to about 7.5%; jojoba oil or castor oil in about 1.0% to about 5.0%; neem oil or tea tree oil in about 0.1% to about 5%; guar gum or methyl cellulose in about 0.1% to about 10%; lecithin in about 1% to about 10%; sodium hydroxide 1M for maintaining pH of 6.0 to 7.0; citric acid in about 0.01% to about 1%; fragrance in about 0.01% to about 3%; and water in q.s to make 100%.
[0058] The present disclosure is based, at least in part, on the premise of unexpected discovery that the waterless antimicrobial handrub composition including an effective amount of the combination of sophorolipid, tea tree oil, and povidone iodine mutually regulate the antimicrobial activity.
[0059] The composition has broad spectrum anti-microbial effect. In an embodiment, the composition is effective against bacteria, bacterial spores, and fungi. In some embodiments, the composition is also effective against methicillin-resistant Staphylococcus aureus.
[0060] The composition is free from harsh antimicrobial chemicals like alcohol, parabens or sodium lauryl sulphate (SLS). In absence of an alcohol base the composition does not rip off the moisture from the skin or leave it dry.
[0061] In another embodiment of the present disclosure, one or more antimicrobial agents might be present. The sophorolipid, chlorhexidine digluconate, octenidine and citric acid demonstrate anti-microbial properties and can also act as preservative.
[0062] Preferably, the composition realized in accordance with embodiments of the present disclosure is prepared in a thick gel or a liquid form with desired viscosity. Most preferably, the composition realized in accordance with embodiments of the present disclosure is prepared in liquid form. Nevertheless, the composition of the present disclosure can be prepared in powdered form and the same is completely within the scope of the present disclosure.
[0063] The composition as realized in accordance with embodiments of the present disclosure can be used in any manner as known to a person skilled in the art. Preferably, the compositions can be directly applied to the bodily surfaces (skin), preferably hand, until the area is wet enough followed by gentle massaging. Subsequently, any cloth or sponge or wipe (dry or wet) can be utilized to clean the bodily surfaces to effect removal of microbes, dirt or soil without use of water. Most preferably, the composition can be used directly to sanitize the hand during surgical procedure. The composition can also be used to clean the patient's body in pre-operative and post-operative conditions. The composition is also useful in the ophthalmic procedures.
[0064] In an embodiment, the composition of the present disclosure exhibits pH ranging from about 6 to about 9, preferably, between about 6.5 to about 8 and most preferably, about 6.9.
[0065] The advantageous composition as realized in accordance with embodiments of the present disclosure leave behind no toxic residue, maintain the smoothness and the pH of the skin, do not cause skin dryness while completely cleansing the skin by removing dirt, sweat, germs and odor, provide excellent moisturizing effect and reduce the microbiological count.
[0066] In an embodiment, the composition has effective cleaning and strong anti-microbial activity. In an embodiment, the composition has a broad-spectrum anti-microbial activity, including bacteria such as gram positive and gram negative bacteria; viruses; bacterial spores; and fungi. In a specific embodiment, the composition is effective against Yeast dermatitis, Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Salmonella abony, Aspergillus brasilensis, and Listeria monocytogenes.
[0067] In some embodiments, the composition shows up to 99.99% anti-microbial efficacy.
[0068] In an embodiment, the composition provides quick action against microbes. In some embodiments, the action may be noticed within 10 seconds of exposure.
[0069] In an embodiment, the present disclosure provides a process of preparing a waterless antimicrobial handrub composition, wherein the process comprises mixing natural antimicrobial agents, bio-surfactants, natural gums or thickener, natural emollient, essential oils, solvent and one or more additive(s); wherein the bio-surfactants is selected from rhamnolipid, sophorolipid, trehalolipids, mannosylerythritol lipids, surfactin, viscosin, ornithine or combinations thereof.
[0070] While the foregoing describes various embodiments of the disclosure, other and further embodiments of the disclosure may be devised without departing from the basic scope thereof. The scope of the invention is determined by the claims that follow. The invention is not limited to the described embodiments, versions or examples, which are included to enable a person having ordinary skill in the art to make and use the invention when combined with information and knowledge available to the person having ordinary skill in the art.
EXAMPLES
[0071] The disclosure will now be illustrated with working examples, which is intended to illustrate the working of disclosure and not intended to take restrictively to imply any limitations on the scope of the present disclosure. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood to one of ordinary skill in the art to which this disclosure belongs. Although methods and materials similar or equivalent to those described herein can be used in the practice of the disclosed methods and compositions, the exemplary methods, devices and materials are described herein. It is to be understood that this disclosure is not limited to particular methods, and experimental conditions described, as such methods and conditions may vary.
[0072] Materials: All raw materials were in house (IH) grades and commercially purchased from third party manufacturers.
[0073] Example 1
[0074] Handrub composition: A handrub composition was prepared as per Table No. 1 below. Dissolved sophorolipid in 50 mL of water in a beaker (B1) and mixed till uniform solution was achieved. Mixed at 500 rpm for about 15 minutes and added citric acid to the above solution and mixed till uniform solution was achieved. Mixed tea tree oil, jojoba oil, and methylcellulose in another beaker of 200 mL(B2). Transferred the solution from beaker B1 to beaker B2 slowly at 500 rpm, and mixed for about 20 minutes. Then added povidone iodine in the beaker and mixed at 500 rpm for 5-10 minutes till uniform solution was achieved. Then fragrance and coloring agent were added and mixed at 500 rpm for about 5-10 minutes. The pH was adjusted to 6.0 to 7.0 using sodium hydroxide by slowly adding and checking the pH while mixing at 500 rpm. The volume was adjusted to 100 mL using distilled water.
Table No. 1: Handrub composition
S. No. Ingredients Name Composition
(% w/w) Functions
1. Sophorolipid 0.01% Bio-surfactant
2. Povidone Iodine (PVP-I) 1.0% Antimicrobial
3. Jojoba Oil 2.0% Emollient
4. Tea Tree Oil 1.0% essential oil
5. Methyl Cellulose 0.2% Gum/Thickener
6. Citric Acid 0.02% Antimicrobial
7. Sodium Hydroxide (1M) 0.1% pH regulator
8. Lavender 1% Fragrance
9. Blue coloring 0.01% coloring agent
10. Water q.s. Solvent
Total 100

Example 2
Anti-microbial activity
[0075] The composition of Example 1 was tested for its anti-microbial activity by a time kill assay. The assay notes the changes in the microbial population within a specified time in presence of the composition. The time kill assays were performed on the ready-to-use formulation. The assays were performed as per the American Standard for Testing and Material protocol ASTM-E2315-16.
[0076] Method:
[0077] TEST PROCEDURE- The working cultures of the test organisms were prepared. 10 mL of the sample was taken with respect to individual test culture. 100 µL of adjusted test cultures were inoculated in individual samples prepared. A contact time of 10 seconds was considered. Using the pour plate technique 1 mL of the composition was pipetted to triplicated petri dishes for each dilution prepared by serial dilution technique and then 20mL of melted SCDA was added. The petri dish was incubated at 30-35°C for 72 hours. The colonies on the petri dishes were counted with the help of colony counter after incubation and the observations were recorded in cfu (colony forming unit).
[0078] Note- The petri dish showing a number of colonies included in a 15-300 for bacterial and fungal of 15-150 range to be used to perform the result calculation.
LOG REDUCTION CALCULATION
[0079] The effectiveness of the disinfection procedure against each challenge organism was calculated for each product as a log10 reduction as follows:
Log10 Reduction per product = Log10 [Positive Control Count] – Log10 [Test Surface Count]
Note: The log reduction was rounded to the nearest 0.1 value.
[0080] For each product concentration and each experimental condition, calculated and recorded the decimal log reduction separately.
ACCEPTANCE CRITERIA
[0081] In case of surface challenge test positive control should yield 50 to 200% recovery of the microorganism in comparison to inoculums added. Bactericidal, fungicidal or yeasticidal activity on surfaces for general purposes is characterized by the concentration of the tested product. Agents should demonstrate a 3 log reduction of vegetative test organisms and mold spores.
[0082] Results: The results of the test are provided in Table No. 2 below:
Table No. 2: Anti-microbial property
S. No. Organism Contact time
(sec) % reduction
1 Yeast dermatitis 10 99.99%
2 S. aureus 10 99.99%
3 Staph. Epidermis 10 99.99%
4 Escherichia coli 10 99.99%
5 Salmonella abony 10 99.99%
6 Aspergillus brasilensis 10 99.99%
7 Listeria monocytogenes 10 99.99%

[0083] Thus, the composition of the present disclosure provides quick action as the anti-microbial efficacy was about 99.99% within 10 seconds of use. Additionally, the composition shows broad spectrum activity.
[0084] Example 3: Disk-diffusion test
[0085] The disinfectant activity of Povidone Iodine (PVP-I) was significantly increased by sophorolipid and tea tree oil in the specific weight percentage ranges. Activity against Staphylococcus Epidermis (ATCC12228): In the well diffusion method, 100 uL of finished product formulation was incubated against the specified microorganisms for 12-14 hours at 37-degree Celsius. The working cultures of the test organisms were prepared. 10 mL of the sample was taken with respect to individual test culture. 100 µL of adjusted test cultures were inoculated in individual samples prepared. Using the pour plate technique, 100uL of the composition of Example 1 was pipetted to triplicated petri dishes for each dilution prepared by serial dilution technique and then 20mL of melted SCDA was added. The petri dish was incubated at 30-35°C for 12-14 hours. Thereafter zone antimicrobial inhibition was analyzed. The results are provided in Table No. 3 below.
Table No. 3: Disk-diffusion tests
S. No Component Antimicrobial Inhibition Zone (cm)
1. Sophorolipid (0.01%) 2.1
2. Sophorolipid (0.01%) +Tea tree oil (1.0 %) 3.0
3. Sophorolipid (0.01%) + PVP-I (1.0 %) 3.0
4. Sophorolipid (0.01%) + PVP-I (1.0 %)+ Tea tree oil (1.0 %) 4.5

[0086] From the results, it was noted that the combination of sophorolipid, tea tree oil, and povidone iodine mutually regulate the antimicrobial activity.
[0087] From the foregoing, it will be appreciated that, although specific embodiments of the invention have been described herein merely for purposes of illustration, various modifications may be made without deviating from the spirit and scope of the invention and should not be construed so as to limit the scope of the invention or the appended claims in any way.

ADVANTAGES OF THE PRESENT INVENTION
[0088] The present disclosure provides a composition that overcomes the deficiencies of the prior art.
[0089] The present disclosure provides a handrub composition that is a broad spectrum anti-microbial and is devoid of harsh chemicals.
[0090] The present disclosure provides a handrub composition that is gentle on the hands of the user, and does not require water for cleaning.
[0091] The present disclosure provides a handrub composition that is effective against methicillin-resistant Staphylococcus aureus.
,CLAIMS:1. A waterless antimicrobial handrub composition comprising natural antimicrobial agents, bio-surfactants, natural gums or thickener, natural emollient and essential oils; wherein the bio-surfactants is selected from rhamnolipid, sophorolipid, trehalolipids, mannosylerythritol lipids, surfactin, viscosin, ornithine or combinations thereof.
2. The composition as claimed in claim 1, wherein the bio-surfactant is present in an amount ranging from 0.001% w/w to 5% w/w of the composition.
3. The composition as claimed in claim 1, wherein the antimicrobial agents is selected from povidone iodine (PVP-I), chlorhexidine digluconate, octenidine dihydrochloride, citric acid, or combinations thereof.
4. The composition as claimed in claim 1, wherein the anti-microbial agent is present in an amount ranging from 0.1% w/w to 10% w/w of the composition.
5. The composition as claimed in claim 1, wherein the natural gum or thickener is selected from guar gum, methyl cellulose, xanthan gum, gum arabic, gum ghatti, locust bean gum, carbopol, cornstarch, gelatin or combinations thereof.
6. The composition as claimed in claim 1, wherein the natural gum or thickener is present in an amount ranging from 0.1% w/w to 20% w/w of the composition.
7. The composition as claimed in claim 1, wherein the natural emollient is selected from castor oil, jojoba oil, squalene, cocoa butter, shea butter, or combinations thereof.
8. The composition as claimed in claim 1, wherein the natural emollient is present in an amount ranging from 0.05% w/w to 10% w/w of the composition.
9. The composition as claimed in claim 1, wherein the essential oils are selected from clove oil, oregano oil, tea tree oil, thyme oil, cinnamon oil, neem oil, lavender oil, lemongrass oil, or combinations thereof.
10. The composition as claimed in claim 1, wherein the essential oils are present in an amount ranging from 0.05% w/w to 10% w/w of the composition.
11. The composition as claimed in claim 1, wherein the composition further comprise one or more additive(s).
12. The composition as claimed in claim 1, wherein the composition comprises sophorolipid; povidone iodine; jojoba oil or castor oil; neem oil or tea tree oil; guar gum or methyl cellulose; lecithin; sodium hydroxide; water; and citric acid.
13. A process of preparing a waterless antimicrobial handrub composition, wherein the process comprises mixing natural antimicrobial agents, bio-surfactants, natural gums or thickener, natural emollient, essential oils, solvent and optionally one or more additive(s); wherein the bio-surfactants is selected from rhamnolipid, sophorolipid, trehalolipids, mannosylerythritol lipids, surfactin, viscosin, ornithine or combinations thereof.