DESC:FIELD OF THE INVENTION
[0001] The present invention relates to a bathing composition for pets. Specifically, the present invention relates to a waterless body bath composition for pets that exhibits excellent cleansing activity along with antimicrobial properties.

BACKGROUND OF THE INVENTION
[0002] Background description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art.
[0003] With the increase in economic and social development, more and more families prefer to raise a pet. The skin and fur of pets generally gets contaminated with pathogens, threatening the health of the owner as well as the pet. It is generally known that pets have a strong dislike for being bathed and this results in an unpleasant experience of bathing a pet which can be traumatic for both the pet and the person bathing the pet. While wet body baths are quite efficient, they require the use of water, which is not always feasible. Further, wet body baths compositions generally contain alcohol and other harsh chemicals, which can cause skin dryness and are as such harmful. Most of the commercially available bathing solutions for pets are only suitable for cleaning the skin and do not contain protection against pathogens.
[0004] Waterless bathing compositions or rinse-free compositions, on the other hand, are a great way to reduce or preclude the use of water in cleansing the skin and other body parts. Since no water is required, waterless bathing compositions provide a quick, simple, and easy solution to bathe pets in cold weather, while traveling or when water is unavailable. Waterless free bathing compositionsare particularly required in situations where the pet is elderly or is recovering from an illness.
[0005] However, existing waterless bathing compositions for pets are largely inefficient in removing body odor, dirt, sweat, and germs. Moreover, the presence of harsh chemicals may cause skin rashes, itching and pH imbalance, leading to other skin problems. So far, the existence of such disadvantages has precluded large scale utilization/commercialization of the waterlessbathing compositions.
[0006] There is, therefore a need in the art for a waterless bathing composition for pets that is devoid of, or at least alleviates, the disadvantages associated with known rinse-free bathing compositions. Need is also felt of waterless bathing composition for pets that exhibits the superior cleansing action over the body along with moisturizing the skin and provides protection against pathogens. Need is also felt of a biodegradable formulation composed of the natural ingredients.

OBJECTS OF THE INVENTION
[0007] An object of the present invention is to provide waterless bathing composition for pets that satisfies the existing needs, as well as emerging ones, and generally overcomes the deficiencies found in the prior art.
[0008] Another object of the present invention is to provide waterless bathing composition for pets that has antimicrobial, antifungal, anti-tick and anti-fleas properties.
[0009] Another object of the present invention is to provide waterless bathing composition for petsthat is free from harsh chemicals.
[0010] Another object of the present invention is to provide waterless bathing composition for pets that exhibits superior cleansing action and has an excellent moisturizing effect.
[0011] Another object of the present invention is to provide waterless bathing composition for pets that is skin-friendly, helps in maintaining the pet’s coat of natural oils and prevents dryness.
[0012] Another object of the present invention is to provide waterless bathing composition for pets that prevents the formation of dandruff, frizzy and matted hair looks.
[0013] Another object of the present invention is to provide waterless bathing composition for pets that can be used without water to clean and disinfect the pets body.
[0014] Another object of the present invention is to provide waterless bathing composition for pets that can be used without water to clean the pets hair.
[0015] Another object of the present invention is to provide waterless bathing composition for pets that provides vitamin to pets hair.

SUMMARY OF THE INVENTION
[0016] This summary is provided to introduce a selection of concepts in a simplified form that is further described below in the detailed description section. This summary is not intended to identify key features or essential features of the claimed subject matter, nor is it intended to be used as an aid in determining the scope of the claimed subject matter.
[0017] The present invention relates to bathing composition for pets. Specifically, the present invention relates to a waterless body bath composition for pets.
[0018] In one aspect, the present invention relates to a waterless body bath composition for pets that exhibits excellent cleansing activity along with antimicrobial, antifungal, anti-tick, and anti-fleas properties.
[0019] In another aspect, the present invention relates to a waterless body bath composition for pets that exhibits superior cleansing action and has an excellent moisturizing effect.
[0020] In another aspect, the present invention relates to a waterless body bath composition for pets that is skin-friendly, helps in maintaining the pet’s coat of natural oils and prevents dryness.
[0021] In yet another aspect, the present invention relates to a waterless body bath composition for pets that prevents the formation of dandruff, frizzy and matted hair looks.
[0022] In another aspect, the present invention relates to a waterless body bath composition for pets comprising surfactants, natural emollients, plant extracts, vitamins and pH regulator.
[0023] In another aspect, the present invention relates to a waterless body bath composition for pets, wherein the composition further comprising water and preservative.
[0024] In yet another aspect, the present invention relates to a waterless body bath composition for pets, wherein the surfactant can be a bio-surfactant, non-ionic surfactant or a combination of both.
[0025] In yet another aspect, the present invention relates to a waterless body bath composition for pets that exhibits anti-inflammatory, anti-bacterial, antiseptic, anti-fungal, antioxidant, moisturising and hair growth stimulation properties.
[0026] In another aspect, the present invention relates to a waterless body bath composition for pets wherein the composition comprises:
a) Rhamnolipid;
b) Coco glucoside;
c) Chamomile extract;
d) Vitamin E;
e) Vitamin D;
f) Aloe vera gel;
g) Vegetable glycerine;
h) Citric acid;
i) Sodium hydroxide; and
j) Water.
[0027] In another aspect, the present invention relates to a waterless body bath composition for pets that can be used without water to clean and disinfect the pets body.
[0028] In yet another aspect, the present invention relates to a waterless body bath composition for pets that can be used without water to clean the pets hair.
[0029] In still another aspect, the present invention relates to a waterless body bath composition for pets that provides vitamin to pets hair.
[0030] Various objects, features, aspects and advantages of the inventive subject matter will become more apparent from the following detailed description of preferred embodiments.

DETAILED DESCRIPTION
[0031] The following is a detailed description of embodiments of the invention. The embodiments are in such detail as to clearly communicate the invention. However, the amount of detail offered is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the scope of the present invention as defined by the appended claims.
[0032] All publications herein are incorporated by reference to the same extent as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference. Where a definition or use of a term in an incorporated reference is inconsistent or contrary to the definition of that term provided herein, the definition of that term provided herein applies and the definition of that term in the reference does not apply.
[0033] Reference throughout this specification to “one embodiment” or “an embodiment” means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrases “in one embodiment” or “in an embodiment” in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.
[0034] In some embodiments, the numbers expressing quantities of ingredients, properties such as concentration, reaction conditions, and so forth, used to describe and claim certain embodiments of the invention are to be understood as being modified in some instances by the term “about.”Accordingly, in some embodiments, the numerical parameters set forth in the written description and attached claims are approximations that can vary depending upon the desired properties sought to be obtained by a particular embodiment.In some embodiments, the numerical parameters should be construed in light of the number of reported significant digits and by applying ordinary rounding techniques.Notwithstanding that the numerical ranges and parameters setting forth the broad scope of some embodiments of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as practicable.The numerical values presented in some embodiments of the invention may contain certain errors necessarily resulting from the standard deviation found in their respective testing measurements.
[0035] As used in the description herein and throughout the claims that follow, the meaning of “a,” “an,” and “the” includes plural reference unless the context clearly dictates otherwise.Also, as used in the description herein, the meaning of “in” includes “in” and “on” unless the context clearly dictates otherwise.
[0036] Unless the context requires otherwise, throughout the specification which follow, the word “comprise” and variations thereof, such as, “comprises” and “comprising” are to be construed in an open, inclusive sense that is as “including, but not limited to.”
[0037] The recitation of ranges of values herein is merely intended to serve as a shorthand method of referring individually to each separate value falling within the range.Unless otherwise indicated herein, each individual value is incorporated into the specification as if it were individually recited herein.All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context.The use of any and all examples, or exemplary language (e.g. “such as”) provided with respect to certain embodiments herein is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention otherwise claimed.No language in the specification should be construed as indicating any non-claimed element essential to the practice of the invention.
[0038] Groupings of alternative elements or embodiments of the invention disclosed herein are not to be construed as limitations.Each group member can be referred to and claimed individually or in any combination with other members of the group or other elements found herein.One or more members of a group can be included in, or deleted from, a group for reasons of convenience and/or patentability.When any such inclusion or deletion occurs, the specification is herein deemed to contain the group as modified thus fulfilling the writtendescription of all Markush groups used in the appended claims.
[0039] The description that follows, and the embodiments described therein, is provided by way of illustration of an example, or examples, of particular embodiments of the principles and aspects of the present invention. These examples are provided for the purposes of explanation, and not of limitation, of those principles and of the invention.
[0040] It should also be appreciated that the present invention can be implementedin numerous ways, including as a system, a method or a device. In this specification, these implementations, or any other form that the invention may take, may be referred to as processes. In general, the order of the steps of the disclosed processes may be altered within the scope of the invention.
[0041] The headings and abstract of the invention provided herein are for convenience only and do not interpret the scope or meaning of the embodiments.
[0042] The following discussion provides many example embodiments of the inventive subject matter. Although each embodiment represents a single combination of inventive elements, the inventive subject matter is considered to include all possible combinations of the disclosed elements. Thus if one embodiment comprises elements A, B, and C, and a second embodiment comprises elements B and D, then the inventive subject matter is also considered to include other remaining combinations of A, B, C, or D, even if not explicitly disclosed.
[0043] Various terms as used herein are shown below. To the extent a term used in a claim is not defined below, it should be given the broadest definition persons in the pertinent art have given that term as reflected in printed publications and issued patents at the time of filing.
[0044] The present invention relates to bathing composition for pets. Specifically, the present invention relates to a waterless body bath composition for pets.
[0045] In one embodiment, the present invention relates to a waterless body bath composition for pets that exhibits excellent cleansing activity along with antimicrobial, antifungal, anti-tick, and anti-fleas properties.
[0046] In another embodiment, the present invention relates to a waterless body bath composition for pets that exhibits superior cleansing action and has an excellent moisturizing effect.
[0047] In still another embodiment, the present invention relates to a waterless body bath composition for pets that is skin-friendly, helps in maintaining the pet’s coat of natural oils and prevents dryness.
[0048] In yet another embodiment, the present invention relates to a waterless body bath composition for pets that prevents the formation of dandruff, frizzy and matted hair looks.
[0049] In another embodiment, the present invention relates to a waterless body bath composition for pets comprising surfactants, natural emollients, plant extracts, vitamins and pH regulator.
[0050] In another embodiment, the present invention relates to a waterless body bath composition for pets, further comprising water and preservative.
[0051] In yet another embodiment, the present invention relates to a waterless body bath composition for pets, wherein the surfactant can be a bio-surfactant, non-ionic surfactant or a combination of both.
[0052] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein bio-surfactant can be selected from rhamnolipid, sophorolipids, trehalolipids, surfactin, viscosin, ornithine and the like.
[0053] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein non-ionic surfactant can be selected from coco-glucoside, cetylalcohol, stearyl alcohol, cetostearyl alcohol, oleyl alcohol, decyl glucoside, lauryl glucoside, triton x-100, nonoxynol-9 and the like.
[0054] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein natural emollients can be selected from vegetable glycerine, chamomile extract, coco-glucoside, shea butter, cocoa butter, argan oil, jojoba oil, cucumber extract, aloe-vera, olive oil, sunflower oil, avocado oil,Butylene glycol and the like.
[0055] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein plant extract can be selected from chamomile extract, aloe vera and the like.
[0056] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein vitamins can be selected from vitamin C, vitamin D, Vitamin E and the like.
[0057] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein pH regulator can be selected from sodium hydroxide, citric acid, lactic acid, triethanolamine, glacial acetic acid, sodium carbonate, acetic acid and the like.
[0058] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein preservative can be selected from citric acid, benzoic acid, sorbic acid,triclosan, triclocarban, phenoxyethanol, benzyl benzoate, benzyl alcohol, zinc pyrithione, benzalkoniumchloride and the like.
[0059] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein the composition comprises:
a) Rhamnolipid;
b) Coco glucoside;
c) Chamomile extract;
d) Vitamin E;
e) Vitamin D ;
f) Aloe vera gel;
g) Vegetable glycerine;
h) Citric acid;
i) Sodium hydroxide; and
j) Water in sufficient quantity to make 100 %.
[0060] In another embodiment, the present invention relates to a waterless body bath composition for that exhibits anti-inflammatory, anti-bacterial, antiseptic, anti-fungal, antioxidant, moisturising and hair growth stimulation properties.
[0061] In yet another embodiment, the present invention relates to a waterless body bath composition for pets wherein rhamnolipid acts as a surfactant, emulsifier and antimicrobial compound.
[0062] In still another embodiment, the present invention relates to a waterless body bath composition for pets wherein coco glucoside acting as a surfactant and emulsifier.
[0063] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein the plant extracts like chamomile extract and aloe vera demonstrate the anti-inflammatory, anti-bacterial and antiseptic properties.
[0064] In yet another embodiment, the present invention relates to a waterless body bath composition for pets wherein citric acid acts as a preservative and antimicrobial compound.
[0065] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein Vitamin D and aloe vera act as hair growth stimulators.
[0066] In still another embodiment, the present invention relates to a waterless body bath composition for pets wherein vitamin E acts as an anti-oxidant.
[0067] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein the composition comprises bio-surfactant in an amount ranging from 0.001% to 1%, preferably in an amount ranging from about 0.01% to about 0.5% by weight of the composition.
[0068] In another embodiment, the present invention relates to a waterless bodybath composition for pets wherein the composition comprises non-ionic surfactant in amount ranging from 0.1% to 10%, preferably in an amount ranging from about 1% to about 5% by weight of the composition.
[0069] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein the composition comprises natural emollients in an amount ranging from 1% to 15%, preferably in an amount ranging from about 2 % to about 7% by weight of the composition.
[0070] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein the composition comprises plant extract in an amount ranging from 0.01% to about 10%, preferably in the amount ranging from about 0.1% to about 5 % by weight of the composition.
[0071] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein the composition comprises vitamins in an amount ranging from 0.01% to 3%, preferably in an amount ranging from about 0.1% to about 2% by weight of the composition.
[0072] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein the pH regulator is added in sufficient quantity to adjust the pH of the solution in the range of 6-9, preferably in the range of 6-7 and most preferably 6.5.
[0073] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein the composition comprises preservative in an amount ranging from 0.01% to 3%, preferably in an amount ranging from about 0.1% to about 1% by weight of the composition.
[0074] In an embodiment, the present invention relates to a waterless body bath composition for pets wherein water is present in a quantity sufficient to bring up the volume of the composition to 100% by weight.
[0075] In another embodiment, the present invention relates to a waterless body bath composition for pets wherein the composition comprises:
a) Rhamnolipid 0.12%;
b) Coco glucoside 2.5%;
c) Chamomile extract 0.5%;
d) Vitamin E 0.01%;
e) Vitamin D 0.2%;
f) Aloe vera gel 1.0%;
g) Vegetable glycerine 3.0%;
h) Citric acid 0.25%;
i) Sodium hydroxide q.s. to adjust pH to 6.5; and
j) Water q.s to make 100 %.
[0076] Preferably, the bathing compositions realized in accordance with embodiments of the present invention are prepared in a semi-solid or a liquid form or as emulsion or gel with desired viscosity. Most preferably, the bathing compositions realized in accordance with embodiments of the present invention are prepared in liquid form. Nevertheless, the bathing compositions of the present invention can be prepared in powdered form and the same are completely within the scope of the present invention.
[0077] In another embodiment, the present invention relates to a waterless body bath composition for pets that can be used without water to clean and disinfect the pets body.
[0078] In yet another embodiment, the present invention relates to a waterless body bath composition for pets that can be used without water to clean the pets hair.
[0079] In still another embodiment, the present invention relates to a waterless body bath composition for pets that provides vitamin to pets hair.
[0080] The term “waterless bathing composition” as used herein throughout the present invention denotes the bathing or cleansing compositions that do not require utilization of water or require minimal amount of water (such as use of wet cloth or sponge or wipe) to effect removal of dirt or soil along with the composition.
[0081] The waterless bathing composition for pets as realized in accordance with embodiments of the present invention can be used in any manner as known to a person skilled in the art. Preferably, the compositions can be directly applied to the bodily surfaces (skin) until the area is wet enough followed by gently massaging. Subsequently, any cloth or sponge or wipe (dry or wet) can be utilized to clean the bodily surfaces to effect removal of dirt or soil and the bathing composition.
[0082] The advantageous waterless bathing compositions as realized in accordance with embodiments of the present invention leave behind no residue, maintain the smoothness and the pH of the skin, do not cause skin dryness while completely cleansing the skin by removing dirt, sweat, germs and odor, provide excellent moisturizing effect and reduce the microbiological count.
[0083] The present invention is based, at least in part, on the premise of unexpected discovery that waterless body bath composition for pets including an effective amount of rhamnolipid, citric acid and coco-glucoside exhibits synergistic activity. Rhamnolipid, citric acid, and coco-glucoside together mutually regulate the antimicrobial activity of the composition (Results depicted in Table 3 in Examples).
[0084] The combination of coco glucoside and rhamnolipid together form a stable emulsion, however, emulsion stability decreased on the removal of one of the components either coco glucoside or rhamnolipid from the solution as shown below:
Attribute Acceptable Result
(After 0 month) Acceptable Result
(After 6 month, with Rhamnolipid and Coco-glucoside) Result
(After 6 month, with Rhamnolipid only) Result
(After 6 month, with Coco glucoside only)
Appearance and emulsion stability Opaque, uniform emulsion with fragrance Opaque, uniform emulsion with fragrance Emulsion was not stable and separated in two layers Emulsion was not stable and separated in two layers

[0085] While the foregoing describes various embodiments of the invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof. The scope of the invention is determined by the claims that follow. The invention is not limited to the described embodiments, versions or examples, which are included to enable a person having ordinary skill in the art to make and use the invention when combined with information and knowledge available to the person having ordinary skill in the art.
[0086] The present invention is further explained in the form of following examples. However, it is to be understood that the followingexamples are merely illustrative and are not to be taken as limitations upon the scope of the invention.
[0087] All chemicals were purcahseed from PK scientific, Karan Vihar Gali No. 1 Kirari Suleman Nagar Delhi-86, and were industrial garde and IH. Biologoical materials were either collected from BBIF-FITT incubation center in IIT Delhi or were procured for the research purposes from CSIR-National Chemical Laboratory, Dr. Homi Bhabha Road , Pune- 411 008, India.
[0088] Example 1: A pet body bath composition
Table 1
Ingredients Weight Pecentage(%)
Rhamnolipid 0.12
Coco glucoside 2.5
Chamomile extract 0.5
Vitamin E 0.01
Vitamin D 0.2
Aloe vera gel 1.0
Vegetable glycerine 3.0
Citric acid 0.25
Sodium hydroxide q.s. to adjust pH to 6.5
Water q.s to make 100%

[0089] Example 2: Preparation of waterless bodybath composition for pets
[0090] The waterless bodybath composition for pets, as described in Example 1 was prepared by the following method:
[0091] 0.12 % wt/wt of Rhamnolipid was added in 50 mL of water and mixed at 300 rpm for 10-20 minutes till uniform solution was achieved. To the same vessel was added 2.5% wt/wt Coco glucoside and stirring continued for 200 rpm for 10-20 minutes, followed by sequential addition of 1.0% wt/wt of Aloe vera gel and 3.0% wt/wt of Vegetable glycerine, followed by addition of 0.25% wt/wt citric acid, 0.01% wt/wt of Vit E and 0.2% wt/wt of Vit D. The solution was stirred after each addition at 200 rpm for 10-20 minutes till uniform solution was achieved. pH of the solution was checked and if required, adjusted to pH of 6.5 to 7 using 10M sodium hydroxide solution. After the required pH was achieved, the volume of the composition was adjusted to 100% wt/wt by using distilled water resulting in the final bodybath composition for pets.

[0092] Example 3: Anti-microbial activity of the composition
[0093] The composition of Example 1 was tested for its anti-microbial activity by a time kill assay. The assay notes the changes in the microbial population within a specified time in presence of the composition. The time kill assays were performed on the ready-to-use formulation. The assays were performed as per the American Standard for Testing and Material protocol ASTM-E2315-16.
[0094] TEST PROCEDURE- The working cultures of the test organisms were prepared. 10 mL of the sample was taken with respect to individual test culture. 100 µL of adjusted test cultures were inoculated in individual samples prepared. A contact time of 20-60 seconds was considered. Using the pour plate technique 1 mL of the formulation was pipetted to triplicated petri dishes for each dilution prepared by serial dilution technique and then 20mL of melted SCDA was added. The petri dish was incubated at 30-35°C for 72 hours. The colonies on the petri dishes were counted with the help of colony counter after incubation and the observations were recorded in cfu (colony forming unit).The petri dish showing a number of colonies included in a 15-300 for bacterial and fungal of 15-150 range was used to perform the result calculation.
[0095] LOG REDUCTION CALCULATION: The effectiveness of the disinfection procedure against each challenge organism was calculated for each product as a log10 reduction as follows:
Log10 Reduction per product = Log10 [Positive Control Count] – Log10 [Test Surface Count]
(The log reduction was rounded to the nearest 0.1 value). For each product concentration and each experimental condition, calculated and recorded the decimal log reduction separately.
[0096] ACCEPTANCE CRITERIA: In case of surface challenge test positive control should yield 50 to 200% recovery of the microorganism in comparison to inoculums added. Bactericidal, fungicidal or yeasticidal activity on surfaces for general purposes is characterized by the concentration of the tested product. Agents should demonstrate a 3 log reduction of vegetative test organisms and mold spores.
[0097] Results: The results of the test are provided in Table 2 as depicted below:
Table 2: Anti-microbial activity of the composition of Example 1
S. No. Organism Contact time
(sec) % reduction
1 Candida L albicans(MTCC 227) 20 99.99%
30 99.99%
60 99.99%
2 S. aureus (ATCC 6538) 20 99.99%
30 99.99%
60 99.99%
3 Staph. Epidermis (ATCC12228) 20 99.99%
30 99.99%
60 99.99%
4 Escherichia coli (ATCC 8739) 20 99.99%
30 99.99%
60 99.99%
5 Salmonella abony (NCTC6017) 20 99.99%
30 99.99%
60 99.99%
6 Aspergillus brasilensis (ATCC16404) 20 99.99%
30 99.99%
60 99.99%
7 Lisleria monocytogenes (ATCC 19111) 20 99.99%
30 99.99%
60 99.99%
[0098] The above results clearly indicate that the composition of the present invention provides quick action as the anti-microbial efficacy was about 99.99% within 20 seconds of use. Additionally, the composition shows broad spectrum activity.

[0099] EXAMPLE 4: Evaluation of disinfectant activity
[00100] Disk-diffusion test: The disinfectant activity was evalutated by Disk diffusion test. In this study, a disk diffusion test was performed on Staph. Epidermis (ATCC12228) using the composition of Example 1 and some comparative compositions as shown in Table 3.
Table 3: Disk-diffusion tests
S. No Component Antimicrobial Inhibition Zone (cm)
1. Rhamnolipid (0.12%) 2.5
2. Rhamnolipid (0.12%) + Coco glucoside (2.5 %) 3.0
3. Rhamnolipid (0.12%) + Citric Acid (0.25 %) 2.5
4. Rhamnolipid (0.12%) + Coco glucoside (2.5 %) + Citric Acid (0.25%) 3.8
[00101] It was concluded from the results that antimicrobial/disinfectant activity of rhamnolipid was significantly increased by addition of coco glucoside and citric acid in the specific weight percentage ranges and the final composition shows synergistic effect. In other words, the combination of rhamnolipid, citric acid, and coco glucoside mutually regulate the antimicrobial activity.
[00102] The foregoing examples are merely illustrative and are not to be taken as limitations upon the scope of the invention. Various changes and modifications to the disclosed embodiments will be apparent to those skilled in the art. Such changes and modifications may be made without departing from the scope of the invention.

ADVANTAGES OF THE PRESENT INVENTION
[00103] The present inventionprovides waterless bathing composition for pets that that has antimicrobial, antifungal, anti-tick, and anti-fleas properties.
[00104] The present inventionprovides waterless bathing composition for pets that is free from harsh chemicals.
[00105] The present invention provides waterless bathing composition for pets that exhibits superior cleansing action and has excellent moisturizing effect.
[00106] The present invention provides waterless bathing composition for pets that is skin-friendly, helps in maintaining the pet’s coat of natural oils and prevents dryness.
[00107] The present invention provides waterless bathing composition for pets that prevents the formation of dandruff, frizzy and matted hair looks.
[00108] The present invention provides a waterless composition that is composed of safe synthetic chemicals, biodegradable chemicals, natural extracts with anti-inflammatory activity, and antimicrobial compounds.
[00109] The present invention provides a waterless composition where rhamnolipid acting as a surfactant, emulsifier and antimicrobial compound.
[00110] The present invention provides a waterless composition where coco-glucoside acting as a surfactant and emulsifier.
[00111] The present invention provides a waterless composition where citric acid acts as a preservative and antimicrobial compound.
[00112] The present invention provides a waterless bathing composition for pets that can be used without water to clean and disinfect the pets body.
[00113] The present invention provides a waterless bathing composition for pets that can be used without water to clean the pets hair.
[00114] The present invention provides a waterless bathing composition for pets that provides vitamin to pets hair.

,CLAIMS:1. A waterless, anti-microbial body bath composition for pets, the composition comprising surfactants, natural emollients, plant extracts, vitamins and pH regulator, wherein surfactant is a bio-surfactant, non-ionic surfactant or a combination of both.

2. The composition as claimed in claim 1, further comprising water and preservative.

3. The composition as claimed in claim 1, wherein bio-surfactant is selected from rhamnolipid, sophorolipids, trehalolipids, surfactin, viscosin and ornithine, present in in an amount ranging from 0.001% to 1% by weight of the composition; non-ionic surfactant is selected from coco-glucoside, cetylalcohol, stearyl alcohol, cetostearyl alcohol, oleyl alcohol, decyl glucoside, lauryl glucoside, Triton X-100 and Nonoxynol-9, present in an amount ranging from 0.1% to 10% by weight of the composition.

4. The composition as claimed in claim 1, wherein natural emollients are selected from vegetable glycerine, chamomile extract, coco-glucoside, shea butter, cocoa butter, argan oil, jojoba oil, cucumber extract, aloe-vera, olive oil, sunflower oil, avocado oil and butylene glycol present in an amount ranging from 1% to 15% by weight of the composition; plant extracts are selected from chamomile extract and aloe vera, present in an amount ranging from 0.01 to about 10% by weight of the composition.

5. The composition as claimed in claim 1, wherein vitamins are selected from vitamin C, vitamin D, vitamin E, present in an amount ranging from 0.01% to 3% by weight of the composition; pH regulator is selected from sodium hydroxide, citric acid, lactic acid, triethanolamine, glacial acetic acid, sodium carbonate and acetic acid, for adjusting the pH of the composition in the range of 6-9.

6. The composition as claimed in claim 2, wherein water is present in a quantity sufficient to bring up the volume of the composition to 100% by weight; preservative is selected from citric acid, benzoic acid, sorbic acid, triclosan, triclocarban, phenoxyethanol, benzyl benzoate, benzyl alcohol, zinc pyrithione and benzalkoniumchloride, present in an amount ranging from 0.01% to 3% by weight of the composition.

7. A waterless, anti-microbial body bath composition for pets, comprising:
a) Rhamnolipid;
b) Coco glucoside;
c) Chamomile extract;
d) Vitamin E;
e) Vitamin D;
f) Aloe vera gel;
g) Vegetable glycerine;
h) Citric acid;
i) Sodium hydroxide; and
j) Water.

8. A waterless, anti-microbial body bath composition for pets, comprising:
a) Rhamnolipid 0.12%;
b) Coco glucoside 2.5%;
c) Chamomile extract 0.5%;
d) Vitamin E 0.01%;
e) Vitamin D 0.2%;
f) Aloe vera gel 1.0%;
g) Vegetable glycerine 3.0%;
h) Citric acid 0.25%;
i) Sodium hydroxide q.s. to adjust pH to 6.5; and
j) Water q.s to make 100 %.

9. The composition as claimed in claims 1 to 8, wherein the composition is in the form of semi-solid or emulsion or liquid or gel.